Ada cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ada
Entrez Gene ID	24165
Full Name	adenosine deaminase
General protein information	Preferred Names adenosine deaminase Names adenosine deaminase adenosine aminohydrolase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q42
Summary	catalyzes the conversion of adenosine to inosine in adenosine metabolism; may act as a neuormodulator in sleep-wake regulation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_130399.2	NP_569083.1	adenosine deaminase

Pathways from BioSystems

Homologies

	ADA	XP_001109908.2
Rattus norvegicus (Norway rat)	Ada	NP_569083.1
Kluyveromyces lactis	KLLA0E23167g	XP_454997.1
Homo sapiens (human)	ADA	NP_000013.2
Danio rerio (zebrafish)	ada	NP_001002646.1
Saccharomyces cerevisiae (baker's yeast)	AAH1	NP_014258.1
Canis lupus familiaris (dog)	ADA	XP_005635156.1
Gallus gallus (chicken)	ADA	NP_001006290.1
Schizosaccharomyces pombe (fission yeast)	dea2	NP_595071.1
Xenopus tropicalis (tropical clawed frog)	ada	NP_001011025.1
Mus musculus (house mouse)	Ada	NP_001258981.1
Pan troglodytes (chimpanzee)	ADA	XP_001144517.1
Bos taurus (cattle)	ADA	NP_776312.1
Caenorhabditis elegans (roundworm)	C06G3.5	NP_501087.1

Gene Ontology
Bibliography

Related articles in PubMed

Quercetin changes purinergic enzyme activities and oxidative profile in platelets of rats with hypothyroidism.
Baldissarelli J, Santi A, Schmatz R, Zanini D, Cardoso AM, Abadalla FH, Thom? GR, Murussi C, Polachini CRN, Delenogare DP, Loro VL, Morsch VM, Schetinger MRC
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie841849-1857(2016 Dec)

Neuroprotective effects of quercetin on memory and anxiogenic-like behavior in diabetic rats: Role of ectonucleotidases and acetylcholinesterase activities.
Maciel RM, Carvalho FB, Olabiyi AA, Schmatz R, Gutierres JM, Stefanello N, Zanini D, Rosa MM, Andrade CM, Rubin MA, Schetinger MR, Morsch VM, Danesi CC, Lopes STA
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie84559-568(2016 Dec)

Neuroinflammation after neonatal hypoxia-ischemia is associated with alterations in the purinergic system: adenosine deaminase 1 isoenzyme is the most predominant after insult.
Pimentel VC, Moretto MB, Oliveira MC, Zanini D, Sebasti?o AM, Schetinger MR
Molecular and cellular biochemistry403(1-2)169-77(2015 May)

E-NTPDase and E-ADA activities in rats experimental infected by Cryptococcus neoformans.
de Azevedo MI, Ferreiro L, Da Silva AS, Tonin AA, Ruchel JB, Rezer JF, Fran?a RT, Zimmermann CE, Leal DB, Duarte MM, Lopes ST, Flores MM, Fighera R, Santurio JM
Veterinary microbiology174(1-2)206-13(2014 Nov)

Role of the A(2B) receptor-adenosine deaminase complex in colonic dysmotility associated with bowel inflammation in rats.
Antonioli L, Fornai M, Awwad O, Giustarini G, Pellegrini C, Tuccori M, Caputi V, Qesari M, Castagliuolo I, Brun P, Giron MC, Scarpignato C, Blandizzi C, Colucci R
British journal of pharmacology171(5)1314-29(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Quercetin decreases NTPDase, 5'-nucleotidase and adenosine deaminase activities in blood platelets of hypothyroid rats, and improves the oxidative profile to reduce platelet aggregation.
Title: Quercetin changes purinergic enzyme activities and oxidative profile in platelets of rats with hypothyroidism.

Quercetin improves memory and anxiogenic-like behavior in diabetic rats by preventing the increase in adenosine deaminase activity seen in streptozocin induced diabetes.
Title: Neuroprotective effects of quercetin on memory and anxiogenic-like behavior in diabetic rats: Role of ectonucleotidases and acetylcholinesterase activities.

The aim of this study was to identify the isoforms of adenosine deaminase (ADA) responsible for the enzymatic activity in the cerebral cortex eight days after hypoxia-ischemia.
Title: Neuroinflammation after neonatal hypoxia-ischemia is associated with alterations in the purinergic system: adenosine deaminase 1 isoenzyme is the most predominant after insult.

E-NTPDase and E-ADA activities in rats experimental infected by Cryptococcus neoformans are involved in the pathogenesis of cryptococcosis.
Title: E-NTPDase and E-ADA activities in rats experimental infected by Cryptococcus neoformans.

These findings suggest that A2B receptors are involved in the regulation of colonic motility and suggest a critical role played by adenosine deaminase in modulating A2B receptor engagement by endogenous adenosine during inflammation.
Title: Role of the A(2B) receptor-adenosine deaminase complex in colonic dysmotility associated with bowel inflammation in rats.

The following Ada gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ada cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00321	NM_130399.2 Latest version!	Rattus norvegicus adenosine deaminase (Ada), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ada ( NM_130399.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_569083.1 Rattus norvegicus adenosine deaminase (Ada), mRNA.

Price & Availability↑

CloneID	ORa00321
Clone ID Related Accession (Same CDS sequence)	NM_130399.2
Accession Version	NM_130399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	adenosine deaminase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088116.1. On Jan 2, 2005 this sequence version replaced NM_130399.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC088116.1, FQ221792.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906349 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCAGA CGCCCGCATT CAACAAGCCC AAAGTAGAGC TGCACGTCCA CCTGGATGGA 
GCCATCAAGC CAGAAACCAT CTTGTACTAT GGCAAGAAGA GAGGAATCGA CCTCCCGGCA 
GATACAGTGG AGGGACTACG CAACATTATC GGCATGGACA AGCCTCTCTC GCTCCCAGAC 
TTCCTGGCCA AGTTCGATTA CTACATGCCT GCCATCGCGG GCTGCAGGGA GGCCATCAAG 
AGAATCGCCT ATGAATTTGT GGAGATGAAG GCAAAGGAAG GCGTGGTGTA CGTGGAAGTT 
CGCTACAGCC CACACCTGCT GGCCAACTCC AAAGTGGACC CAATCCCCTG GAACCAGGCC 
GAAGGAGACC TCACCCCTGA TGAGGTTGTG GATCTTGTAA ACCAGGGCCT GCAGGAGGGA 
GAGCAAGCAT TCGGCATCAA GGTCCGGTCC ATCTTGTGCT GCATGCGCCA CCAGCCCAGC 
TGGTCCCCTG AGGTGTTGGA GCTGTGTAAG AAGTACCATC AGAAGACCGT GGTGGCTATG 
GACTTGGCTG GGGATGAGAC CATTGAAGGA AGCAGCCTTT TCCCGGGCCA CGTGGAAGCC 
TACGAGGGTG CAGTGAAGGA TGGCATTCAC CGGACTGTCC ATGCTGGAGA GGTGGGCTCT 
GCTGAGGTGG TGCGAGAGGC TGTGGACATA CTCAAGACAG AGAGAGTGGG GCACGGCTAC 
CACACAATAG AGGATGAAGC CCTCTACAAC AGGCTACTGA AAGAAAACAT GCACTTTGAG 
GTCTGCCCCT GGTCCAGCTA CCTCACTGGC GCCTGGAATC CCAAAACGAC GCATGCAGTT 
GTTCGCTTCA AAGATGATCA GGCCAACTAC TCCCTCAACT CGGACGACCC CCTCATCTTC 
AAGTCCACCG TGGACACTGA CTACCAGATG GTGAAAAAAG ACATGGGCTT CACTGAAGAG 
GAGTTCAAGA GACTGAACAT CAATGCGGCG AAGTCAAGCT TCCTCCCAGA GGACGAGAAG 
AAGGAACTTC TGGAACGGCT CTACAAGGAA TACCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569083.1
CDS	77..1135
Translation	MAQTPAFNKPKVELHVHLDGAIKPETILYYGKKRGIDLPADTVEGLRNIIGMDKPLSLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVDPIPWNQAEGDLTPDEVVDLVNQGLQEGEQAFGIKVRSILCCMRHQPSWSPEVLELCKKYHQKTVVAMDLAGDETIEGSSLFPGHVEAYEGAVKDGIHRTVHAGEVGSAEVVREAVDILKTERVGHGYHTIEDEALYNRLLKENMHFEVCPWSSYLTGAWNPKTTHAVVRFKDDQANYSLNSDDPLIFKSTVDTDYQMVKKDMGFTEEEFKRLNINAAKSSFLPEDEKKELLERLYKEYQ

Target ORF information:

RefSeq Version	NM_130399.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adenosine deaminase (Ada), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130399.2

ORF Insert Sequence:

ATGGCCCAGA CGCCCGCATT CAACAAGCCC AAAGTAGAGC TGCACGTCCA CCTGGATGGA 
GCCATCAAGC CAGAAACCAT CTTGTACTAT GGCAAGAAGA GAGGAATCGA CCTCCCGGCA 
GATACAGTGG AGGGACTACG CAACATTATC GGCATGGACA AGCCTCTCTC GCTCCCAGAC 
TTCCTGGCCA AGTTCGATTA CTACATGCCT GCCATCGCGG GCTGCAGGGA GGCCATCAAG 
AGAATCGCCT ATGAATTTGT GGAGATGAAG GCAAAGGAAG GCGTGGTGTA CGTGGAAGTT 
CGCTACAGCC CACACCTGCT GGCCAACTCC AAAGTGGACC CAATCCCCTG GAACCAGGCC 
GAAGGAGACC TCACCCCTGA TGAGGTTGTG GATCTTGTAA ACCAGGGCCT GCAGGAGGGA 
GAGCAAGCAT TCGGCATCAA GGTCCGGTCC ATCTTGTGCT GCATGCGCCA CCAGCCCAGC 
TGGTCCCCTG AGGTGTTGGA GCTGTGTAAG AAGTACCATC AGAAGACCGT GGTGGCTATG 
GACTTGGCTG GGGATGAGAC CATTGAAGGA AGCAGCCTTT TCCCGGGCCA CGTGGAAGCC 
TACGAGGGTG CAGTGAAGGA TGGCATTCAC CGGACTGTCC ATGCTGGAGA GGTGGGCTCT 
GCTGAGGTGG TGCGAGAGGC TGTGGACATA CTCAAGACAG AGAGAGTGGG GCACGGCTAC 
CACACAATAG AGGATGAAGC CCTCTACAAC AGGCTACTGA AAGAAAACAT GCACTTTGAG 
GTCTGCCCCT GGTCCAGCTA CCTCACTGGC GCCTGGAATC CCAAAACGAC GCATGCAGTT 
GTTCGCTTCA AAGATGATCA GGCCAACTAC TCCCTCAACT CGGACGACCC CCTCATCTTC 
AAGTCCACCG TGGACACTGA CTACCAGATG GTGAAAAAAG ACATGGGCTT CACTGAAGAG 
GAGTTCAAGA GACTGAACAT CAATGCGGCG AAGTCAAGCT TCCTCCCAGA GGACGAGAAG 
AAGGAACTTC TGGAACGGCT CTACAAGGAA TACCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quercetin changes purinergic enzyme activities and oxidative profile in platelets of rats with hypothyroidism.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie841849-1857(2016 Dec)
Baldissarelli J,Santi A,Schmatz R,Zanini D,Cardoso AM,Abadalla FH,Thom? GR,Murussi C,Polachini CRN,Delenogare DP,Loro VL,Morsch VM,Schetinger MRC

Neuroprotective effects of quercetin on memory and anxiogenic-like behavior in diabetic rats: Role of ectonucleotidases and acetylcholinesterase activities.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie84559-568(2016 Dec)
Maciel RM,Carvalho FB,Olabiyi AA,Schmatz R,Gutierres JM,Stefanello N,Zanini D,Rosa MM,Andrade CM,Rubin MA,Schetinger MR,Morsch VM,Danesi CC,Lopes STA

Neuroinflammation after neonatal hypoxia-ischemia is associated with alterations in the purinergic system: adenosine deaminase 1 isoenzyme is the most predominant after insult.
Molecular and cellular biochemistry403(1-2)169-77(2015 May)
Pimentel VC,Moretto MB,Oliveira MC,Zanini D,Sebasti?o AM,Schetinger MR

E-NTPDase and E-ADA activities in rats experimental infected by Cryptococcus neoformans.
Veterinary microbiology174(1-2)206-13(2014 Nov)
de Azevedo MI,Ferreiro L,Da Silva AS,Tonin AA,Ruchel JB,Rezer JF,Fran?a RT,Zimmermann CE,Leal DB,Duarte MM,Lopes ST,Flores MM,Fighera R,Santurio JM

Role of the A(2B) receptor-adenosine deaminase complex in colonic dysmotility associated with bowel inflammation in rats.
British journal of pharmacology171(5)1314-29(2014 Mar)
Antonioli L,Fornai M,Awwad O,Giustarini G,Pellegrini C,Tuccori M,Caputi V,Qesari M,Castagliuolo I,Brun P,Giron MC,Scarpignato C,Blandizzi C,Colucci R

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Ada cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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