Bsnd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bsnd
Entrez Gene ID	192675
Full Name	barttin CLCNK type accessory beta subunit
General protein information	Preferred Names barttin CLCNK type accessory beta subunit Names barttin Bartter syndrome, infantile, with sensorineural deafness (Barttin) barttin CLCNK-type chloride channel accessory beta subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q34
Summary	binds to and activates ClC-K channels and is required for adequate tubular salt reabsorption in the kidney [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138979.2	NP_620435.1	barttin

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	BSND	XP_003434700.1
Xenopus tropicalis (tropical clawed frog)	bsnd	XP_002931511.1
Pan troglodytes (chimpanzee)	BSND	XP_001143070.2
Homo sapiens (human)	BSND	NP_476517.1
Bos taurus (cattle)	BSND	NP_001180013.1
Rattus norvegicus (Norway rat)	Bsnd	NP_620435.1
	BSND	XP_001114228.1
Mus musculus (house mouse)	Bsnd	NP_536706.2

Gene Ontology
Bibliography

Related articles in PubMed

Molecular architecture of the stria vascularis membrane transport system, which is essential for physiological functions of the mammalian cochlea.
Uetsuka S, Ogata G, Nagamori S, Isozumi N, Nin F, Yoshida T, Komune S, Kitahara T, Kikkawa Y, Inohara H, Kanai Y, Hibino H
The European journal of neuroscience42(3)1984-2002(2015 Aug)

Parallel down-regulation of chloride channel CLC-K1 and barttin mRNA in the thin ascending limb of the rat nephron by furosemide.
Wolf K, Meier-Meitinger M, Bergler T, Castrop H, Vitzthum H, Riegger GA, Kurtz A, Kr?mer BK
Pflugers Archiv : European journal of physiology446(6)665-71(2003 Sep)

Barttin increases surface expression and changes current properties of ClC-K channels.
Waldegger S, Jeck N, Barth P, Peters M, Vitzthum H, Wolf K, Kurtz A, Konrad M, Seyberth HW
Pflugers Archiv : European journal of physiology444(3)411-8(2002 Jun)

Mutation of BSND causes Bartter syndrome with sensorineural deafness and kidney failure.
Birkenh?ger R, Otto E, Sch?rmann MJ, Vollmer M, Ruf EM, Maier-Lutz I, Beekmann F, Fekete A, Omran H, Feldmann D, Milford DV, Jeck N, Konrad M, Landau D, Knoers NV, Antignac C, Sudbrak R, Kispert A, Hildebrandt F
Nature genetics29(3)310-4(2001 Nov)

Barttin is a Cl- channel beta-subunit crucial for renal Cl- reabsorption and inner ear K+ secretion.
Est?vez R, Boettger T, Stein V, Birkenh?ger R, Otto E, Hildebrandt F, Jentsch TJ
Nature414(6863)558-61(2001 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Bsnd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bsnd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13885	NM_138979.2 Latest version!	Rattus norvegicus barttin CLCNK type accessory subunit beta (Bsnd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bsnd ( NM_138979.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620435.1 Rattus norvegicus barttin CLCNK type accessory subunit beta (Bsnd), mRNA.

Price & Availability↑

CloneID	ORa13885
Clone ID Related Accession (Same CDS sequence)	NM_138979.2
Accession Version	NM_138979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	barttin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081725.1. On Sep 16, 2004 this sequence version replaced NM_138979.1. ##Evidence-Data-START## Transcript exon combination :: BC081725.1, AJ421029.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGACG AGAAGACCTT CCGAATCGGT TTCATCGTGC TGGGCCTTTT CCTGCTATCC 
CTGGGCACAT TCCTTATGAG CCACGATCGA CCCCAAGTCT ACGGCACCTT CTATGCCATG 
GGCAGCATCA TGGTGATTGG GGGTGTCCTC TGGAGCATGT GCCAATGCTA TCCTAAGATC 
ACCTTTGTGC CGGCAGACTC TGACTTCCAA GGCATGTTGT CCCCAAAGGC CCTGAGCCTG 
CTGGAGACTG GGCTTTCAGA AGTAAAGAGC CCCCAGCCCC CCTACGTCAG GCTGTGGGAA 
GAAGCCGCTT ATGACCAGAG CCTCCCAGAC TTCACTCACA TCCAAATGAA GGTCATGGGC 
TACAGTGAGG ACCCCAGGCC ACTGCTGGCC CCCGAACTGA AGACAGGAAC CAGCAGTGCC 
AAGGAAGGCG AGCCTCATTC TGCTCAGACC TGGATGGAGG CTGCGGTGGT CGTCCATAGA 
GAGTTGGATG AGAAGGAGGG AGAGAAATCT CGTTCTCAGA GCAGCCCTCC AGCGTGTTCC 
CAAGGCTCTG CACCCTTGGC GTCATTTCAC GATGACTTGG ACGTGGGTTC TAGCGAAGGC 
CGCAGCCCCC AGCCATCCCC ACCTGACAGA GATGAGGCAC ACCTGCAGGT GCCCTGGGCC 
AGCAGAGGTC CACTGGATCG CTTCGGTGAC TTTGCTCTGA TTGATGACAC CCCTATATCA 
GAGGACATGG GCCTGGAGGG GCAGGCACAG GAGGAAGCTC TACCCAGCAA GCAACCATGG 
TCCCTGAGGA TGAAGGAGGA GACTGTCCAG GCAGGTGCAG AAGAACCAGA GCAGGAGGAG 
GAAGATCTGT ACTACGGGCT GCCCGACAGC CCTGGGGACC CCCTCCCTGA CAAAGAGTTG 
GGCTTTGAGC CTGATGTCCA GGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620435.1
CDS	200..1126
Translation	MADEKTFRIGFIVLGLFLLSLGTFLMSHDRPQVYGTFYAMGSIMVIGGVLWSMCQCYPKITFVPADSDFQGMLSPKALSLLETGLSEVKSPQPPYVRLWEEAAYDQSLPDFTHIQMKVMGYSEDPRPLLAPELKTGTSSAKEGEPHSAQTWMEAAVVVHRELDEKEGEKSRSQSSPPACSQGSAPLASFHDDLDVGSSEGRSPQPSPPDRDEAHLQVPWASRGPLDRFGDFALIDDTPISEDMGLEGQAQEEALPSKQPWSLRMKEETVQAGAEEPEQEEEDLYYGLPDSPGDPLPDKELGFEPDVQG

Target ORF information:

RefSeq Version	NM_138979.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus barttin CLCNK type accessory subunit beta (Bsnd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138979.2

ORF Insert Sequence:

ATGGCTGACG AGAAGACCTT CCGAATCGGT TTCATCGTGC TGGGCCTTTT CCTGCTATCC 
CTGGGCACAT TCCTTATGAG CCACGATCGA CCCCAAGTCT ACGGCACCTT CTATGCCATG 
GGCAGCATCA TGGTGATTGG GGGTGTCCTC TGGAGCATGT GCCAATGCTA TCCTAAGATC 
ACCTTTGTGC CGGCAGACTC TGACTTCCAA GGCATGTTGT CCCCAAAGGC CCTGAGCCTG 
CTGGAGACTG GGCTTTCAGA AGTAAAGAGC CCCCAGCCCC CCTACGTCAG GCTGTGGGAA 
GAAGCCGCTT ATGACCAGAG CCTCCCAGAC TTCACTCACA TCCAAATGAA GGTCATGGGC 
TACAGTGAGG ACCCCAGGCC ACTGCTGGCC CCCGAACTGA AGACAGGAAC CAGCAGTGCC 
AAGGAAGGCG AGCCTCATTC TGCTCAGACC TGGATGGAGG CTGCGGTGGT CGTCCATAGA 
GAGTTGGATG AGAAGGAGGG AGAGAAATCT CGTTCTCAGA GCAGCCCTCC AGCGTGTTCC 
CAAGGCTCTG CACCCTTGGC GTCATTTCAC GATGACTTGG ACGTGGGTTC TAGCGAAGGC 
CGCAGCCCCC AGCCATCCCC ACCTGACAGA GATGAGGCAC ACCTGCAGGT GCCCTGGGCC 
AGCAGAGGTC CACTGGATCG CTTCGGTGAC TTTGCTCTGA TTGATGACAC CCCTATATCA 
GAGGACATGG GCCTGGAGGG GCAGGCACAG GAGGAAGCTC TACCCAGCAA GCAACCATGG 
TCCCTGAGGA TGAAGGAGGA GACTGTCCAG GCAGGTGCAG AAGAACCAGA GCAGGAGGAG 
GAAGATCTGT ACTACGGGCT GCCCGACAGC CCTGGGGACC CCCTCCCTGA CAAAGAGTTG 
GGCTTTGAGC CTGATGTCCA GGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular architecture of the stria vascularis membrane transport system, which is essential for physiological functions of the mammalian cochlea.
The European journal of neuroscience42(3)1984-2002(2015 Aug)
Uetsuka S,Ogata G,Nagamori S,Isozumi N,Nin F,Yoshida T,Komune S,Kitahara T,Kikkawa Y,Inohara H,Kanai Y,Hibino H

Parallel down-regulation of chloride channel CLC-K1 and barttin mRNA in the thin ascending limb of the rat nephron by furosemide.
Pflugers Archiv : European journal of physiology446(6)665-71(2003 Sep)
Wolf K,Meier-Meitinger M,Bergler T,Castrop H,Vitzthum H,Riegger GA,Kurtz A,Kr?mer BK

Barttin increases surface expression and changes current properties of ClC-K channels.
Pflugers Archiv : European journal of physiology444(3)411-8(2002 Jun)
Waldegger S,Jeck N,Barth P,Peters M,Vitzthum H,Wolf K,Kurtz A,Konrad M,Seyberth HW

Mutation of BSND causes Bartter syndrome with sensorineural deafness and kidney failure.
Nature genetics29(3)310-4(2001 Nov)
Birkenh?ger R,Otto E,Sch?rmann MJ,Vollmer M,Ruf EM,Maier-Lutz I,Beekmann F,Fekete A,Omran H,Feldmann D,Milford DV,Jeck N,Konrad M,Landau D,Knoers NV,Antignac C,Sudbrak R,Kispert A,Hildebrandt F

Barttin is a Cl- channel beta-subunit crucial for renal Cl- reabsorption and inner ear K+ secretion.
Nature414(6863)558-61(2001 Nov)
Est?vez R,Boettger T,Stein V,Birkenh?ger R,Otto E,Hildebrandt F,Jentsch TJ

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Bsnd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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