Phf5a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phf5a
Entrez Gene ID	192246
Full Name	PHD finger protein 5A
Synonyms	Ac2-246
General protein information	Preferred Names PHD finger protein 5A Names PHD finger-like domain-containing protein 5A PHD finger-like domain protein 5A SF3b14b splicing factor 3B-associated 14 kDa protein transcription factor INI
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34
Summary	zinc-finger transcription factor; promotes the up-regulation of the connexin43 gene by estrogen [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138888.1	NP_620243.1	PHD finger-like domain-containing protein 5A

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	RDS3	NP_015419.1
Arabidopsis thaliana (thale cress)	AT1G07170	NP_001077473.1
Oryza sativa (rice)	Os05g0367000	NP_001055334.1
Xenopus tropicalis (tropical clawed frog)	phf5a	NP_001123712.1
Pan troglodytes (chimpanzee)	PHF5A	XP_003317310.1
Drosophila melanogaster (fruit fly)	CG9548	NP_609038.1
Bos taurus (cattle)	PHF5A	NP_001076851.1
Gallus gallus (chicken)	PHF5A	NP_001264305.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009905	XP_319026.1
Caenorhabditis elegans (roundworm)	phf-5	NP_001022909.1
Kluyveromyces lactis	KLLA0C15675g	XP_452900.1
Magnaporthe oryzae (rice blast fungus)	MGG_05173	XP_003712711.1
Arabidopsis thaliana (thale cress)	AT2G30000	NP_565691.1
Oryza sativa (rice)	Os04g0663300	NP_001054163.1
Mus musculus (house mouse)	Phf5a	NP_081013.1
Danio rerio (zebrafish)	phf5a	NP_775373.1
Schizosaccharomyces pombe (fission yeast)	ini1	NP_593792.1
Neurospora crassa	NCU00348	XP_957819.2
Homo sapiens (human)	PHF5A	NP_116147.1
	LOC711683	XP_002798444.1
Canis lupus familiaris (dog)	PHF5A	XP_531718.1
Rattus norvegicus (Norway rat)	Phf5a	NP_620243.1
Eremothecium gossypii	AGOS_ABR103C	NP_983050.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of the human activated spliceosome in three conformational states.
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell research(2018 Jan)

Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.
Teng T, Tsai JH, Puyang X, Seiler M, Peng S, Prajapati S, Aird D, Buonamici S, Caleb B, Chan B, Corson L, Feala J, Fekkes P, Gerard B, Karr C, Korpal M, Liu X, T Lowe J, Mizui Y, Palacino J, Park E, Smith PG, Subramanian V, Wu ZJ, Zou J, Yu L, Chicas A, Warmuth M, Larsen N, Zhu P
Nature communications815522(2017 May)

Molecular Architecture of SF3b and Structural Consequences of Its Cancer-Related Mutations.
Cretu C, Schmitzov? J, Ponce-Salvatierra A, Dybkov O, De Laurentiis EI, Sharma K, Will CL, Urlaub H, L?hrmann R, Pena V
Molecular cell64(2)307-319(2016 Oct)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

that Ini's physiological role in myometrium is to modulate the response of the cx43 gene to estrogen.
Title: Ini, a small nuclear protein that enhances the response of the connexin43 gene to estrogen.

The following Phf5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phf5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01881	NM_138888.1 Latest version!	Rattus norvegicus PHD finger protein 5A (Phf5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phf5a ( NM_138888.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620243.1 Rattus norvegicus PHD finger protein 5A (Phf5a), mRNA.

Price & Availability↑

CloneID	ORa01881
Clone ID Related Accession (Same CDS sequence)	NM_138888.1
Accession Version	NM_138888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	PHD finger-like domain-containing protein 5A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF495522.1. ##Evidence-Data-START## Transcript exon combination :: AF495522.1, BC063807.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906349 [ECO:0000348] ##Evidence-Data-END##

ATGGCTAAAC ATCATCCAGA TTTGATTTTC TGCCGCAAGC AGGCTGGTGT AGCTATCGGA 
AGACTGTGTG AAAAATGTGA TGGCAAATGT GTGATCTGCG ATTCCTACGT GCGTCCCTGT 
ACCCTGGTTC GCATATGTGA TGAGTGTAAC TATGGATCCT ACCAGGGCCG CTGCGTGATC 
TGTGGAGGTC CCGGAGTCTC TGATGCCTAC TATTGTAAAG AGTGCACCAT TCAGGAGAAG 
GATAGAGATG GTTGCCCAAA GATTGTCAAT TTGGGGAGCT CTAAGACAGA CCTGTTCTAT 
GAGCGCAAAA AATACGGTTT CAAGAAGAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620243.1
CDS	1..333
Translation	MAKHHPDLIFCRKQAGVAIGRLCEKCDGKCVICDSYVRPCTLVRICDECNYGSYQGRCVICGGPGVSDAYYCKECTIQEKDRDGCPKIVNLGSSKTDLFYERKKYGFKKR

Target ORF information:

RefSeq Version	NM_138888.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PHD finger protein 5A (Phf5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138888.1

ORF Insert Sequence:

ATGGCTAAAC ATCATCCAGA TTTGATTTTC TGCCGCAAGC AGGCTGGTGT AGCTATCGGA 
AGACTGTGTG AAAAATGTGA TGGCAAATGT GTGATCTGCG ATTCCTACGT GCGTCCCTGT 
ACCCTGGTTC GCATATGTGA TGAGTGTAAC TATGGATCCT ACCAGGGCCG CTGCGTGATC 
TGTGGAGGTC CCGGAGTCTC TGATGCCTAC TATTGTAAAG AGTGCACCAT TCAGGAGAAG 
GATAGAGATG GTTGCCCAAA GATTGTCAAT TTGGGGAGCT CTAAGACAGA CCTGTTCTAT 
GAGCGCAAAA AATACGGTTT CAAGAAGAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of the human activated spliceosome in three conformational states.
Cell research(2018 Jan)
Zhang X,Yan C,Zhan X,Li L,Lei J,Shi Y

Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.
Nature communications815522(2017 May)
Teng T,Tsai JH,Puyang X,Seiler M,Peng S,Prajapati S,Aird D,Buonamici S,Caleb B,Chan B,Corson L,Feala J,Fekkes P,Gerard B,Karr C,Korpal M,Liu X,T Lowe J,Mizui Y,Palacino J,Park E,Smith PG,Subramanian V,Wu ZJ,Zou J,Yu L,Chicas A,Warmuth M,Larsen N,Zhu P

Molecular Architecture of SF3b and Structural Consequences of Its Cancer-Related Mutations.
Molecular cell64(2)307-319(2016 Oct)
Cretu C,Schmitzov? J,Ponce-Salvatierra A,Dybkov O,De Laurentiis EI,Sharma K,Will CL,Urlaub H,L?hrmann R,Pena V

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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Phf5a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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