Akr1d1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Akr1d1
Entrez Gene ID	192242
Full Name	aldo-keto reductase family 1, member D1
General protein information	Preferred Names aldo-keto reductase family 1, member D1 Names 3-oxo-5-beta-steroid 4-dehydrogenase aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) delta(4)-3-ketosteroid 5-beta-reductase delta(4)-3-oxosteroid 5-beta-reductase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q22
Summary	enzyme that catalyzes the reduction of key intermediates during bile acid biosynthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006236285.1	XP_006236347.1	3-oxo-5-beta-steroid 4-dehydrogenase isoform X1
NM_138884.1	NP_620239.1	aldo-keto reductase family 1 member D1

Pathways from BioSystems

Homologies

Homo sapiens (human)	AKR1D1	NP_005980.1
	AKR1D1	XP_001107157.1
Canis lupus familiaris (dog)	AKR1D1	XP_005629673.1
Bos taurus (cattle)	AKR1D1	NP_001179287.1
Mus musculus (house mouse)	Akr1d1	NP_663339.1
Rattus norvegicus (Norway rat)	Akr1d1	NP_620239.1
Xenopus tropicalis (tropical clawed frog)	akr1d1	NP_001025609.1
Pan troglodytes (chimpanzee)	AKR1D1	XP_001148679.1
Gallus gallus (chicken)	AKR1D1	NP_001264322.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of the 37-kd rat liver protein that forms an acetaldehyde adduct in vivo as delta 4-3-ketosteroid 5 beta-reductase.
Zhu Y, Fillenwarth MJ, Crabb D, Lumeng L, Lin RC
Hepatology (Baltimore, Md.)23(1)115-22(1996 Jan)

Cloning and expression of cDNA of human delta 4-3-oxosteroid 5 beta-reductase and substrate specificity of the expressed enzyme.
Kondo KH, Kai MH, Setoguchi Y, Eggertsen G, Sj?blom P, Setoguchi T, Okuda KI, Bj?rkhem I
European journal of biochemistry219(1-2)357-63(1994 Jan)

delta 4-3-Oxosteroid 5 beta-reductase. Structure and function.
Onishi Y, Noshiro M, Shimosato T, Okuda K
Biological chemistry Hoppe-Seyler372(12)1039-49(1991 Dec)

Molecular cloning and sequence analysis of cDNA encoding delta 4-3-ketosteroid 5 beta-reductase of rat liver.
Onishi Y, Noshiro M, Shimosato T, Okuda K
FEBS letters283(2)215-8(1991 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Akr1d1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Akr1d1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01866	NM_138884.1 Latest version!	Rattus norvegicus aldo-keto reductase family 1, member D1 (Akr1d1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa27146	XM_006236285.1 Latest version!	Rattus norvegicus aldo-keto reductase family 1, member D1 (Akr1d1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Akr1d1 ( NM_138884.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620239.1 Rattus norvegicus aldo-keto reductase family 1, member D1 (Akr1d1), mRNA.

Price & Availability↑

CloneID	ORa01866
Clone ID Related Accession (Same CDS sequence)	NM_138884.1
Accession Version	NM_138884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	aldo-keto reductase family 1 member D1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D17309.1. On Dec 17, 2003 this sequence version replaced XM_346688.1. ##Evidence-Data-START## Transcript exon combination :: D17309.1, FQ219730.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689598, SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGAACCTCA GCACTGCAAA CCACCACATA CCCCTAAATG ATGGTAACAG CATTCCGATC 
ATCGGGCTTG GAACCTACTC AGACCCTAGA CCGGTACCTG GCAAGACCTT TATAGCAGTG 
AAGACAGCTA TTGACGAGGG GTACCGGCAT ATTGATGGGG CCTACGTCTA CCGAAATGAA 
CATGAAGTCG GTGAGGCCAT CAGAGAAAAG GTGGCAGAAG GGAAGGTAAA GAGGGAAGAG 
ATTTTCTACT GTGGAAAGTT ATGGAGTACA GACCATGATC CAGAGATGGT CCGCCCAGCC 
CTGGAAAGGA CCCTGCAGAC CCTCAAGCTA GATTACATAG ACCTTTATAT CATTGAGATG 
CCCATGGCCT TTAAGCCTGG AGAGGAATTT TATCCTAAAG ATGAGAATGG CCGAGTGATA 
TACCACAAAT CAAATCTGTG TGCCACGTGG GAGGCACTGG AAGCTTGCAA AGACGCTGGC 
TTGGTGAAAT CCCTTGGGGT GTCTAATTTT AACCGCAGGC AGCTGGAGGT CATCTTGAAC 
AAGCCAGGAC TCAAGTACAA GCCTGTCACC AACCAGGTGG AGTGCCACCC GTATTTCACC 
CAGACAAAAC TCCTTGAAGT TTCTGCCAGC AGCATGACAT CGTTCATTGT CGCATACAGC 
CCCTTAGGGA CCTGTCGCAA CCCGTTATGG GTGAATGTAT CTTCTCCACC CTTGTTAAAG 
GATGAACTCC TAACCTCGCT GGGGAAAAAG TACAATAAGA CACAAGCTCA AATTGTGTTG 
CGTTTCGACA TCCAGCGAGG GTTGGTTGTC ATCCCTAAAA GTACTACCCC TGAAAGGATC 
AAAGAAAACT TTCAGATCTT TGACTTCTCT CTCACCAAAG AAGAAATGAA GGACATTGAA 
GCCTTGAATA AAAACGTGCG CTTTGTGGAG ATGCTCATGT GGAGTGATCA TCCTGAATAC 
CCATTTCATG ACGAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620239.1
CDS	41..1021
Translation	MNLSTANHHIPLNDGNSIPIIGLGTYSDPRPVPGKTFIAVKTAIDEGYRHIDGAYVYRNEHEVGEAIREKVAEGKVKREEIFYCGKLWSTDHDPEMVRPALERTLQTLKLDYIDLYIIEMPMAFKPGEEFYPKDENGRVIYHKSNLCATWEALEACKDAGLVKSLGVSNFNRRQLEVILNKPGLKYKPVTNQVECHPYFTQTKLLEVSASSMTSFIVAYSPLGTCRNPLWVNVSSPPLLKDELLTSLGKKYNKTQAQIVLRFDIQRGLVVIPKSTTPERIKENFQIFDFSLTKEEMKDIEALNKNVRFVEMLMWSDHPEYPFHDEY

Target ORF information:

RefSeq Version	NM_138884.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aldo-keto reductase family 1, member D1 (Akr1d1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138884.1

ORF Insert Sequence:

ATGAACCTCA GCACTGCAAA CCACCACATA CCCCTAAATG ATGGTAACAG CATTCCGATC 
ATCGGGCTTG GAACCTACTC AGACCCTAGA CCGGTACCTG GCAAGACCTT TATAGCAGTG 
AAGACAGCTA TTGACGAGGG GTACCGGCAT ATTGATGGGG CCTACGTCTA CCGAAATGAA 
CATGAAGTCG GTGAGGCCAT CAGAGAAAAG GTGGCAGAAG GGAAGGTAAA GAGGGAAGAG 
ATTTTCTACT GTGGAAAGTT ATGGAGTACA GACCATGATC CAGAGATGGT CCGCCCAGCC 
CTGGAAAGGA CCCTGCAGAC CCTCAAGCTA GATTACATAG ACCTTTATAT CATTGAGATG 
CCCATGGCCT TTAAGCCTGG AGAGGAATTT TATCCTAAAG ATGAGAATGG CCGAGTGATA 
TACCACAAAT CAAATCTGTG TGCCACGTGG GAGGCACTGG AAGCTTGCAA AGACGCTGGC 
TTGGTGAAAT CCCTTGGGGT GTCTAATTTT AACCGCAGGC AGCTGGAGGT CATCTTGAAC 
AAGCCAGGAC TCAAGTACAA GCCTGTCACC AACCAGGTGG AGTGCCACCC GTATTTCACC 
CAGACAAAAC TCCTTGAAGT TTCTGCCAGC AGCATGACAT CGTTCATTGT CGCATACAGC 
CCCTTAGGGA CCTGTCGCAA CCCGTTATGG GTGAATGTAT CTTCTCCACC CTTGTTAAAG 
GATGAACTCC TAACCTCGCT GGGGAAAAAG TACAATAAGA CACAAGCTCA AATTGTGTTG 
CGTTTCGACA TCCAGCGAGG GTTGGTTGTC ATCCCTAAAA GTACTACCCC TGAAAGGATC 
AAAGAAAACT TTCAGATCTT TGACTTCTCT CTCACCAAAG AAGAAATGAA GGACATTGAA 
GCCTTGAATA AAAACGTGCG CTTTGTGGAG ATGCTCATGT GGAGTGATCA TCCTGAATAC 
CCATTTCATG ACGAATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akr1d1 ( XM_006236285.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236347.1 Rattus norvegicus aldo-keto reductase family 1, member D1 (Akr1d1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa27146
Clone ID Related Accession (Same CDS sequence)	XM_006236285.1
Accession Version	XM_006236285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	3-oxo-5-beta-steroid 4-dehydrogenase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAATG AGGCAGTCCA CCACAGTTTC TCTTACCTCT ACGATCTTCC AATCATCAAA 
GTACCCGGCA AGACCTTTAT AGCAGTGAAG ACAGCTATTG ACGAGGGGTA CCGGCATATT 
GATGGGGCCT ACGTCTACCG AAATGAACAT GAAGTTGGTG AGGCCATCAG AGAAAAGGTG 
GCAGAAGGGA AGGTAAAGAG GGAAGAGATT TTCTACTGTG GAAAGTTATG GAGTACAGAC 
CATGATCCAG AGATGGTCCG CCCAGCCCTG GAAAGGACCC TGCAGACCCT CAAGCTAGAT 
TACATAGACC TTTATATCAT TGAGATGCCC ATGGCCTTTA AGCCTGGAGA GGAATTTTAT 
CCTAAAGATG AGAATGGCCG AGTGATATAC CACAAATCAA ATCTGTGTGC CACGTGGGAG 
GCACTGGAAG CTTGCAAAGA CGCTGGCTTG GTGAAATCCC TTGGGGTGTC TAATTTTAAC 
CGCAGGCAGC TGGAGGTCAT CTTGAACAAG CCAGGACTCA AGTACAAGCC TGTCACCAAC 
CAGGTGGAGT GCCACCCGTA TTTCACCCAG ACAAAACTCT TGAAGTTCTG CCAGCAGCAT 
GACATCGTCA TTGTCGCATA CAGCCCCTTA GGGACCTGTC GCAACCCGTT ATGGGTGAAT 
GTATCTTCTC CACCCTTGTT AAAGGATGAA CTCCTAACCT CGCTGGGGAA AAAGTACAAT 
AAGACACAAG CTCAAATTGT GTTGCGTTTC GACATCCAGC GAGGGTTGGT TGTCATCCCT 
AAAAGTACTA CCCCTGAAAG GATCAAAGAA AACTTTCAGA TCTTTGACTT CTCTCTCACC 
AAAGAAGAAA TGAAGGACAT TGAAGCCTTG AATAAAAACG TGCGCTTTGT GGAGATGCTC 
ATGTGGAGTG ATCATCCTGA ATACCCATTT CATGACGAAT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236347.1
CDS	94..1038
Translation	MLNEAVHHSFSYLYDLPIIKVPGKTFIAVKTAIDEGYRHIDGAYVYRNEHEVGEAIREKVAEGKVKREEIFYCGKLWSTDHDPEMVRPALERTLQTLKLDYIDLYIIEMPMAFKPGEEFYPKDENGRVIYHKSNLCATWEALEACKDAGLVKSLGVSNFNRRQLEVILNKPGLKYKPVTNQVECHPYFTQTKLLKFCQQHDIVIVAYSPLGTCRNPLWVNVSSPPLLKDELLTSLGKKYNKTQAQIVLRFDIQRGLVVIPKSTTPERIKENFQIFDFSLTKEEMKDIEALNKNVRFVEMLMWSDHPEYPFHDEY

Target ORF information:

RefSeq Version	XM_006236285.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aldo-keto reductase family 1, member D1 (Akr1d1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236285.1

ORF Insert Sequence:

ATGCTGAATG AGGCAGTCCA CCACAGTTTC TCTTACCTCT ACGATCTTCC AATCATCAAA 
GTACCCGGCA AGACCTTTAT AGCAGTGAAG ACAGCTATTG ACGAGGGGTA CCGGCATATT 
GATGGGGCCT ACGTCTACCG AAATGAACAT GAAGTTGGTG AGGCCATCAG AGAAAAGGTG 
GCAGAAGGGA AGGTAAAGAG GGAAGAGATT TTCTACTGTG GAAAGTTATG GAGTACAGAC 
CATGATCCAG AGATGGTCCG CCCAGCCCTG GAAAGGACCC TGCAGACCCT CAAGCTAGAT 
TACATAGACC TTTATATCAT TGAGATGCCC ATGGCCTTTA AGCCTGGAGA GGAATTTTAT 
CCTAAAGATG AGAATGGCCG AGTGATATAC CACAAATCAA ATCTGTGTGC CACGTGGGAG 
GCACTGGAAG CTTGCAAAGA CGCTGGCTTG GTGAAATCCC TTGGGGTGTC TAATTTTAAC 
CGCAGGCAGC TGGAGGTCAT CTTGAACAAG CCAGGACTCA AGTACAAGCC TGTCACCAAC 
CAGGTGGAGT GCCACCCGTA TTTCACCCAG ACAAAACTCT TGAAGTTCTG CCAGCAGCAT 
GACATCGTCA TTGTCGCATA CAGCCCCTTA GGGACCTGTC GCAACCCGTT ATGGGTGAAT 
GTATCTTCTC CACCCTTGTT AAAGGATGAA CTCCTAACCT CGCTGGGGAA AAAGTACAAT 
AAGACACAAG CTCAAATTGT GTTGCGTTTC GACATCCAGC GAGGGTTGGT TGTCATCCCT 
AAAAGTACTA CCCCTGAAAG GATCAAAGAA AACTTTCAGA TCTTTGACTT CTCTCTCACC 
AAAGAAGAAA TGAAGGACAT TGAAGCCTTG AATAAAAACG TGCGCTTTGT GGAGATGCTC 
ATGTGGAGTG ATCATCCTGA ATACCCATTT CATGACGAAT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of the 37-kd rat liver protein that forms an acetaldehyde adduct in vivo as delta 4-3-ketosteroid 5 beta-reductase.
Hepatology (Baltimore, Md.)23(1)115-22(1996 Jan)
Zhu Y,Fillenwarth MJ,Crabb D,Lumeng L,Lin RC

Cloning and expression of cDNA of human delta 4-3-oxosteroid 5 beta-reductase and substrate specificity of the expressed enzyme.
European journal of biochemistry219(1-2)357-63(1994 Jan)
Kondo KH,Kai MH,Setoguchi Y,Eggertsen G,Sj?blom P,Setoguchi T,Okuda KI,Bj?rkhem I

delta 4-3-Oxosteroid 5 beta-reductase. Structure and function.
Biological chemistry Hoppe-Seyler372(12)1039-49(1991 Dec)
Onishi Y,Noshiro M,Shimosato T,Okuda K

Molecular cloning and sequence analysis of cDNA encoding delta 4-3-ketosteroid 5 beta-reductase of rat liver.
FEBS letters283(2)215-8(1991 Jun)
Onishi Y,Noshiro M,Shimosato T,Okuda K

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Akr1d1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報