Mpst cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mpst
Entrez Gene ID	192172
Full Name	mercaptopyruvate sulfurtransferase
Synonyms	Mst
General protein information	Preferred Names mercaptopyruvate sulfurtransferase Names 3-mercaptopyruvate sulfurtransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34
Summary	catalyzes the formation of thiocyanide and pyruvate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138843.1	NP_620198.1	3-mercaptopyruvate sulfurtransferase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MPST	XP_005625964.1
Bos taurus (cattle)	MPST	NP_001029463.2
Kluyveromyces lactis	KLLA0C09966g	XP_452643.1
Eremothecium gossypii	AGOS_AAL096W	NP_982446.1
Schizosaccharomyces pombe (fission yeast)	tum1	XP_001713164.1
Neurospora crassa	NCU06017	XP_959418.1
Arabidopsis thaliana (thale cress)	MST1	NP_565203.1
Rattus norvegicus (Norway rat)	Mpst	NP_620198.1
Danio rerio (zebrafish)	zgc:162544	NP_001082815.1
Pan troglodytes (chimpanzee)	MPST	XP_001159671.1
Magnaporthe oryzae (rice blast fungus)	MGG_04087	XP_003719751.1
Arabidopsis thaliana (thale cress)	RDH2	NP_849675.1
	MPST	XP_002798401.1
Gallus gallus (chicken)	MPST	NP_001264306.1
Homo sapiens (human)	MPST	NP_066949.2
Mus musculus (house mouse)	Mpst	NP_619611.3
Saccharomyces cerevisiae (baker's yeast)	TUM1	NP_014894.3
Oryza sativa (rice)	Os12g0608600	NP_001067237.1
Xenopus tropicalis (tropical clawed frog)	mpst	NP_001005646.1

Gene Ontology
Bibliography

Related articles in PubMed

Alternative pathway of H
Nagahara N, Koike S, Nirasawa T, Kimura H, Ogasawara Y
Biochemical and biophysical research communications(2018 Jan)

Discovery and Mechanistic Characterization of Selective Inhibitors of H
Hanaoka K, Sasakura K, Suwanai Y, Toma-Fukai S, Shimamoto K, Takano Y, Shibuya N, Terai T, Komatsu T, Ueno T, Ogasawara Y, Tsuchiya Y, Watanabe Y, Kimura H, Wang C, Uchiyama M, Kojima H, Okabe T, Urano Y, Shimizu T, Nagano T
Scientific reports740227(2017 Jan)

MPST but not CSE is the primary regulator of hydrogen sulfide production and function in the coronary artery.
Kuo MM, Kim DH, Jandu S, Bergman Y, Tan S, Wang H, Pandey DR, Abraham TP, Shoukas AA, Berkowitz DE, Santhanam L
American journal of physiology. Heart and circulatory physiology310(1)H71-9(2016 Jan)

Contribution of cysteine aminotransferase and mercaptopyruvate sulfurtransferase to hydrogen sulfide production in peripheral neurons.
Miyamoto R, Otsuguro K, Yamaguchi S, Ito S
Journal of neurochemistry130(1)29-40(2014 Jul)

Chronic intermittent hypoxia promotes expression of 3-mercaptopyruvate sulfurtransferase in adult rat medulla oblongata.
Li M, Nie L, Hu Y, Yan X, Xue L, Chen L, Zhou H, Zheng Y
Autonomic neuroscience : basic & clinical179(1-2)84-9(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

3-Mercaptopyruvate sulphurtransferase and not cystathionine gamma-lyase is the primary regulator of coronary artery hydrogen sulfide production and function.
Title: MPST but not CSE is the primary regulator of hydrogen sulfide production and function in the coronary artery.

Hydrogen sulfide (H2 S) is synthesized in peripheral neurons through two mitochondrial enzymes, cysteine aminotransferase (CAT) and mercaptopyruvate sulfurtransferase (MPST).
Title: Contribution of cysteine aminotransferase and mercaptopyruvate sulfurtransferase to hydrogen sulfide production in peripheral neurons.

3MST exists in the neurons of medullary respiratory nuclei and its expression can be up-regulated by chronic intermittent hypoxia in adult rat
Title: Chronic intermittent hypoxia promotes expression of 3-mercaptopyruvate sulfurtransferase in adult rat medulla oblongata.

SiRNA silencing of 3-MPST reduced basal bioenergetic parameters and prevented the stimulating effect of 3-MP on mitochondrial bioenergetics.
Title: Intramitochondrial hydrogen sulfide production by 3-mercaptopyruvate sulfurtransferase maintains mitochondrial electron flow and supports cellular bioenergetics.

observations indicate that 3MST is probably not responsible for the increased production of H2S following permanent middle cerebral artery occlusion.
Title: Brain 3-Mercaptopyruvate Sulfurtransferase (3MST): Cellular Localization and Downregulation after Acute Stroke.

The following Mpst gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mpst cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12482	NM_138843.1 Latest version!	Rattus norvegicus mercaptopyruvate sulfurtransferase (Mpst), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mpst ( NM_138843.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620198.1 Rattus norvegicus mercaptopyruvate sulfurtransferase (Mpst), mRNA.

Price & Availability↑

CloneID	ORa12482
Clone ID Related Accession (Same CDS sequence)	NM_138843.1
Accession Version	NM_138843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	3-mercaptopyruvate sulfurtransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D50564.1. On Dec 17, 2003 this sequence version replaced XM_346800.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D50564.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCTGCCC CACAGCTTTT CCGAGCTCTG GTATCTGCTC AGTGGGTGGC GGAGGCTCTG 
AAATCCCCTC GCGCCTCTCA GCCGCTGAAG TTACTGGATG CGTCCTGGTA CCTACCCAAG 
CTGGGCCGCG ATGCGCGACG CGAGTTTGAG GAGCGCCACA TCCCGGGCGC CGCCTTCTTT 
GACATCGATC GTTGCAGCGA CCACACATCG CCCTACGACC ACATGCTGCC TAGTGCCACG 
CATTTCGCCG ACTACGCGGG CAGCTTGGGC GTGAGTGCCG CTACACACGT CGTGATCTAC 
GACGGCAGCG ACCAGGGCCT CTATTCCGCT CCGAGGGTCT GGTGGATGTT CCGCGCCTTC 
GGCCACCACT CTGTGTCGTT GCTGGACGGC GGCTTCCGCT ATTGGCTGAG CCAGAACCTG 
CCAATTAGCT CTGGCAAAAG CCCCTCGGAA CCTGCGGAGT TCTGCGCGCA GCTCGATCCC 
TCCTTCATCA AGACCCACGA GGATATCCTG GAGAACTTGG ATGCCCGGCG CTTCCAGGTA 
GTGGACGCCC GCGCAGCTGG CCGTTTCCAA GGCACACAGC CAGAACCCCG AGATGGCATC 
GAACCTGGAC ACATCCCTGG CTCAGTAAAC ATCCCATTCA CTGAATTCCT GACCAGTGAA 
GGTCTGGAGA AGAGCCCAGA GGAGATCCAA CGCCTGTTCC AGGAGAAGAA GGTGGACCTG 
TCCAAGCCCT TGGTAGCCAC ATGCGGCTCC GGTGTCACAG CCTGCCACGT GGTCCTGGGG 
GCCTTCCTCT GTGGCAAACC CGATGTGCCT GTCTACGATG GCTCCTGGGT GGAGTGGTAC 
ATGCGTGCCC AACCGGAGCA CGTCATCTCT CAGGGCCGGG GGAAGACCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620198.1
CDS	210..1103
Translation	MAAPQLFRALVSAQWVAEALKSPRASQPLKLLDASWYLPKLGRDARREFEERHIPGAAFFDIDRCSDHTSPYDHMLPSATHFADYAGSLGVSAATHVVIYDGSDQGLYSAPRVWWMFRAFGHHSVSLLDGGFRYWLSQNLPISSGKSPSEPAEFCAQLDPSFIKTHEDILENLDARRFQVVDARAAGRFQGTQPEPRDGIEPGHIPGSVNIPFTEFLTSEGLEKSPEEIQRLFQEKKVDLSKPLVATCGSGVTACHVVLGAFLCGKPDVPVYDGSWVEWYMRAQPEHVISQGRGKTL

Target ORF information:

RefSeq Version	NM_138843.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mercaptopyruvate sulfurtransferase (Mpst), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138843.1

ORF Insert Sequence:

ATGGCTGCCC CACAGCTTTT CCGAGCTCTG GTATCTGCTC AGTGGGTGGC GGAGGCTCTG 
AAATCCCCTC GCGCCTCTCA GCCGCTGAAG TTACTGGATG CGTCCTGGTA CCTACCCAAG 
CTGGGCCGCG ATGCGCGACG CGAGTTTGAG GAGCGCCACA TCCCGGGCGC CGCCTTCTTT 
GACATCGATC GTTGCAGCGA CCACACATCG CCCTACGACC ACATGCTGCC TAGTGCCACG 
CATTTCGCCG ACTACGCGGG CAGCTTGGGC GTGAGTGCCG CTACACACGT CGTGATCTAC 
GACGGCAGCG ACCAGGGCCT CTATTCCGCT CCGAGGGTCT GGTGGATGTT CCGCGCCTTC 
GGCCACCACT CTGTGTCGTT GCTGGACGGC GGCTTCCGCT ATTGGCTGAG CCAGAACCTG 
CCAATTAGCT CTGGCAAAAG CCCCTCGGAA CCTGCGGAGT TCTGCGCGCA GCTCGATCCC 
TCCTTCATCA AGACCCACGA GGATATCCTG GAGAACTTGG ATGCCCGGCG CTTCCAGGTA 
GTGGACGCCC GCGCAGCTGG CCGTTTCCAA GGCACACAGC CAGAACCCCG AGATGGCATC 
GAACCTGGAC ACATCCCTGG CTCAGTAAAC ATCCCATTCA CTGAATTCCT GACCAGTGAA 
GGTCTGGAGA AGAGCCCAGA GGAGATCCAA CGCCTGTTCC AGGAGAAGAA GGTGGACCTG 
TCCAAGCCCT TGGTAGCCAC ATGCGGCTCC GGTGTCACAG CCTGCCACGT GGTCCTGGGG 
GCCTTCCTCT GTGGCAAACC CGATGTGCCT GTCTACGATG GCTCCTGGGT GGAGTGGTAC 
ATGCGTGCCC AACCGGAGCA CGTCATCTCT CAGGGCCGGG GGAAGACCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Alternative pathway of H
Biochemical and biophysical research communications(2018 Jan)
Nagahara N,Koike S,Nirasawa T,Kimura H,Ogasawara Y

Discovery and Mechanistic Characterization of Selective Inhibitors of H
Scientific reports740227(2017 Jan)
Hanaoka K,Sasakura K,Suwanai Y,Toma-Fukai S,Shimamoto K,Takano Y,Shibuya N,Terai T,Komatsu T,Ueno T,Ogasawara Y,Tsuchiya Y,Watanabe Y,Kimura H,Wang C,Uchiyama M,Kojima H,Okabe T,Urano Y,Shimizu T,Nagano T

MPST but not CSE is the primary regulator of hydrogen sulfide production and function in the coronary artery.
American journal of physiology. Heart and circulatory physiology310(1)H71-9(2016 Jan)
Kuo MM,Kim DH,Jandu S,Bergman Y,Tan S,Wang H,Pandey DR,Abraham TP,Shoukas AA,Berkowitz DE,Santhanam L

Contribution of cysteine aminotransferase and mercaptopyruvate sulfurtransferase to hydrogen sulfide production in peripheral neurons.
Journal of neurochemistry130(1)29-40(2014 Jul)
Miyamoto R,Otsuguro K,Yamaguchi S,Ito S

Chronic intermittent hypoxia promotes expression of 3-mercaptopyruvate sulfurtransferase in adult rat medulla oblongata.
Autonomic neuroscience : basic & clinical179(1-2)84-9(2013 Dec)
Li M,Nie L,Hu Y,Yan X,Xue L,Chen L,Zhou H,Zheng Y

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Mpst cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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