Sftpb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sftpb
Entrez Gene ID	192155
Full Name	surfactant protein B
Synonyms	Sp-b
General protein information	Preferred Names surfactant protein B Names pulmonary surfactant-associated protein B pulmonary surfactant-associated proteolipid SPL(Phe) surfactant associated protein B surfactant, pulmonary-associated protein B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q31
Summary	acts to lower surface tension in lung alveoli [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138842.1	NP_620197.1	pulmonary surfactant-associated protein B precursor
XM_006236657.3	XP_006236719.1	pulmonary surfactant-associated protein B isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SFTPB	XP_005630540.1
	SFTPB	XP_001089841.1
Pan troglodytes (chimpanzee)	SFTPB	XP_001168379.2
Mus musculus (house mouse)	Sftpb	NP_001269000.1
Homo sapiens (human)	SFTPB	NP_000533.3
Xenopus tropicalis (tropical clawed frog)	sftpb	XP_002932589.2
Bos taurus (cattle)	SFTPB	NP_001068779.1
Rattus norvegicus (Norway rat)	Sftpb	NP_620197.1

Gene Ontology
Bibliography

Related articles in PubMed

A small key unlocks a heavy door: The essential function of the small hydrophobic proteins SP-B and SP-C to trigger adsorption of pulmonary surfactant lamellar bodies.
Hobi N, Giolai M, Olmeda B, Miklavc P, Felder E, Walther P, Dietl P, Frick M, P?rez-Gil J, Haller T
Biochimica et biophysica acta1863(8)2124-34(2016 08)

Activation of the GLP-1 Receptor by Liraglutide Increases ACE2 Expression, Reversing Right Ventricle Hypertrophy, and Improving the Production of SP-A and SP-B in the Lungs of Type 1 Diabetes Rats.
Roman?-P?rez M, Outeiri?o-Iglesias V, Moya CM, Santisteban P, Gonz?lez-Mat?as LC, Vigo E, Mallo F
Endocrinology156(10)3559-69(2015 Oct)

Sox9 plays multiple roles in the lung epithelium during branching morphogenesis.
Rockich BE, Hrycaj SM, Shih HP, Nagy MS, Ferguson MA, Kopp JL, Sander M, Wellik DM, Spence JR
Proceedings of the National Academy of Sciences of the United States of America110(47)E4456-64(2013 Nov)

Functional importance of the NH2-terminal insertion sequence of lung surfactant protein B.
Frey SL, Pocivavsek L, Waring AJ, Walther FJ, Hernandez-Juviel JM, Ruchala P, Lee KY
American journal of physiology. Lung cellular and molecular physiology298(3)L335-47(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SP-B is present on the surface of exocytosed surfactant particles. Antibodies against SP-B stabilize the released particles when contacting the air-liquid interface, precluding their transformation and the formation of surface films. SP-B acts as an essential, preformed molecular key in the initial stages of surfactant unpacking and surface film formation.
Title: A small key unlocks a heavy door: The essential function of the small hydrophobic proteins SP-B and SP-C to trigger adsorption of pulmonary surfactant lamellar bodies.

Liraglutide activates the GLP1 receptor, increasing ACE2 expression, reversing right ventricle hypertrophy, and improving the production of SP-A and SP-B in the lungs of Type 1 Diabetes rats
Title: Activation of the GLP-1 Receptor by Liraglutide Increases ACE2 Expression, Reversing Right Ventricle Hypertrophy, and Improving the Production of SP-A and SP-B in the Lungs of Type 1 Diabetes Rats.

The role of the SP-B NH(2) terminus in the formation and stability of cylindrical structures, specifically the distribution of lipid stack height, width, and density with four SP-B truncation peptides, was studied.
Title: Functional importance of the NH2-terminal insertion sequence of lung surfactant protein B.

alterations of surfactant are a consequence of the ventilation strategy that impair the biophysical activity of this material and thereby contribute directly to lung dysfunction over time
Title: Alterations to surfactant precede physiological deterioration during high tidal volume ventilation.

VEGF increased expression of SP-b mRNA in preterm rat lung.
Title: High-dose vascular endothelial growth factor increases surfactant protein gene expressions in preterm rat lung.

The following Sftpb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sftpb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01705	NM_138842.1 Latest version!	Rattus norvegicus surfactant protein B (Sftpb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa27349	XM_006236657.3 Latest version!	Rattus norvegicus surfactant protein B (Sftpb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sftpb ( NM_138842.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620197.1 Rattus norvegicus surfactant protein B (Sftpb), mRNA.

Price & Availability↑

CloneID	ORa01705
Clone ID Related Accession (Same CDS sequence)	NM_138842.1
Accession Version	NM_138842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	pulmonary surfactant-associated protein B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X14778.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X14778.1, BC072466.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMN02642778 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAGT TACATCTGCA GTGGCTACTG CTCCTTCCTA CACTCTGCAG CCTAGGCGCA 
GCTACAGAGT CGGCCTCGTC CCCGGATTGT GCACAAGGCC CTAAATTCTG GTGCCAAAGC 
CTGGAGCAAG CGATACAGTG CAGAGCCCTG GGGCACTGCC TACAGGAAGT CTGGGGACAT 
GCAGGAGCTA ATGACCTGTG CCAAGAGTGT GAGGATATTG TCCACCTCCT CACAAAGATG 
ACCAAGGAAG ACGCTTTCCA GGACACGATC CGGAAGTTCC TGGAACAAGA ATGTGATATC 
CTACCCTTGA AGCTGCTTGT GCCCCGGTGT CGCCAAGTGC TTGATGTCTA CCTGCCCCTG 
GTTATCGACT ACTTCCAGGG CCAGATTAAA CCCAAAGCCA TCTGCAGTCA TGTGGGCCTG 
TGCCCACTTG GGCAGACTAA GCCAGAGCAG AAGCCCGAGA TGCTGGATGC CATTCCAAAC 
CCTCTGCTGA ACAAGCTGGT CCTCCCTGCG CTGCCAGGAG CCTTCTTGGC AAGGCCTGGG 
CCTCACACAC AGGACCTCTC TGAGCAACAG CTCCCCATCC CTCTGCCCTT CTGCTGGCTT 
TGCAGGACTC TGATCAAGCG GGTCCAAGCT GTGATTCCCA AGGGTGTGCT GGCCGTGGCT 
GTGTCCCAGG TGTGCCACGT GGTACCCCTG GTGGTGGGTG GCATCTGCCA GTGCCTGGCT 
GAGCGTTACA CAGTACTTCT ACTAGATGCA CTGCTGGGTC GTGTGGTGCC CCAGCTAGTC 
TGTGGCCTGG TCCTCCGATG TTCCACTGCA GATGCCATTG GCCCAGCCCT CCCTGCTCTG 
GAGCCTCTGA TTGAAAAATG GCCACTGCAA GACACTGAGT GCCATTTCTG CAAGTCTGTG 
ATCAACCAGG CCTGGAACAC CAGTGAACAG GCTATGCCAC AGGCAATGCA CCAGGCCTGC 
CTTCGCTTCT GGCTAGACAG GCAAAAGTGT GAGCAGTTTG TGGAACAGCA CATGCCCCAG 
CTGCTGGCCC TGGTGCCTAG GAGCCAGGAT GCCCACACCT CCTGCCAGGC CCTTGGAGTA 
TGTGAGGCCC CGGCCAGCCC TCTGCAATGC TTCCAAACCC CACACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620197.1
CDS	7..1137
Translation	MAKLHLQWLLLLPTLCSLGAATESASSPDCAQGPKFWCQSLEQAIQCRALGHCLQEVWGHAGANDLCQECEDIVHLLTKMTKEDAFQDTIRKFLEQECDILPLKLLVPRCRQVLDVYLPLVIDYFQGQIKPKAICSHVGLCPLGQTKPEQKPEMLDAIPNPLLNKLVLPALPGAFLARPGPHTQDLSEQQLPIPLPFCWLCRTLIKRVQAVIPKGVLAVAVSQVCHVVPLVVGGICQCLAERYTVLLLDALLGRVVPQLVCGLVLRCSTADAIGPALPALEPLIEKWPLQDTECHFCKSVINQAWNTSEQAMPQAMHQACLRFWLDRQKCEQFVEQHMPQLLALVPRSQDAHTSCQALGVCEAPASPLQCFQTPHL

Target ORF information:

RefSeq Version	NM_138842.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus surfactant protein B (Sftpb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138842.1

ORF Insert Sequence:

ATGGCCAAGT TACATCTGCA GTGGCTACTG CTCCTTCCTA CACTCTGCAG CCTAGGCGCA 
GCTACAGAGT CGGCCTCGTC CCCGGATTGT GCACAAGGCC CTAAATTCTG GTGCCAAAGC 
CTGGAGCAAG CGATACAGTG CAGAGCCCTG GGGCACTGCC TACAGGAAGT CTGGGGACAT 
GCAGGAGCTA ATGACCTGTG CCAAGAGTGT GAGGATATTG TCCACCTCCT CACAAAGATG 
ACCAAGGAAG ACGCTTTCCA GGACACGATC CGGAAGTTCC TGGAACAAGA ATGTGATATC 
CTACCCTTGA AGCTGCTTGT GCCCCGGTGT CGCCAAGTGC TTGATGTCTA CCTGCCCCTG 
GTTATCGACT ACTTCCAGGG CCAGATTAAA CCCAAAGCCA TCTGCAGTCA TGTGGGCCTG 
TGCCCACTTG GGCAGACTAA GCCAGAGCAG AAGCCCGAGA TGCTGGATGC CATTCCAAAC 
CCTCTGCTGA ACAAGCTGGT CCTCCCTGCG CTGCCAGGAG CCTTCTTGGC AAGGCCTGGG 
CCTCACACAC AGGACCTCTC TGAGCAACAG CTCCCCATCC CTCTGCCCTT CTGCTGGCTT 
TGCAGGACTC TGATCAAGCG GGTCCAAGCT GTGATTCCCA AGGGTGTGCT GGCCGTGGCT 
GTGTCCCAGG TGTGCCACGT GGTACCCCTG GTGGTGGGTG GCATCTGCCA GTGCCTGGCT 
GAGCGTTACA CAGTACTTCT ACTAGATGCA CTGCTGGGTC GTGTGGTGCC CCAGCTAGTC 
TGTGGCCTGG TCCTCCGATG TTCCACTGCA GATGCCATTG GCCCAGCCCT CCCTGCTCTG 
GAGCCTCTGA TTGAAAAATG GCCACTGCAA GACACTGAGT GCCATTTCTG CAAGTCTGTG 
ATCAACCAGG CCTGGAACAC CAGTGAACAG GCTATGCCAC AGGCAATGCA CCAGGCCTGC 
CTTCGCTTCT GGCTAGACAG GCAAAAGTGT GAGCAGTTTG TGGAACAGCA CATGCCCCAG 
CTGCTGGCCC TGGTGCCTAG GAGCCAGGAT GCCCACACCT CCTGCCAGGC CCTTGGAGTA 
TGTGAGGCCC CGGCCAGCCC TCTGCAATGC TTCCAAACCC CACACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sftpb ( XM_006236657.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236719.1 Rattus norvegicus surfactant protein B (Sftpb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa27349
Clone ID Related Accession (Same CDS sequence)	XM_006236657.3
Accession Version	XM_006236657.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pulmonary surfactant-associated protein B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236657.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGT TACATCTGCA GTGGCTACTG CTCCTTCCTA CACTCTGCAG CCTAGGCGCA 
GCTACAGAGT CGGCCTCGTC CCCGGATTGT GCACAAGGCC CTAAATTCTG GTGCCAAAGC 
CTGGAGCAAG CGATACAGTG CAGAGCCCTG GGGCACTGCC TACAGGAAGT CTGGGGACAT 
GCAGGAGCTA ATGACCTGTG CCAAGAGTGT GAGGATATTG TCCACCTCCT CACAAAGATG 
ACCAAGGAAG ACGCTTTCCA GGACACGATC CGGAAGTTCC TGGAACAAGA ATGTGATATC 
CTACCCTTGA AGCTGCTTGT GCCCCGGTGT CGCCAAGTGC TTGATGTCTA CCTGCCCCTG 
GTTATCGACT ACTTCCAGGG CCAGATTAAA CCCAAAGCCA TCTGCAGTCA TGTGGGCCTG 
TGCCCACTTG GGCAGACTAA GCCAGAGCAG AAGCCCGAGA TGCTGGATGC CATTCCAAAC 
CCTCTGCTGA ACAAGCTGGT CCTCCCTGCG CTGCCAGGAG CCTTCTTGGC AAGGCCTGGG 
CCTCACACAC AGGACCTCTC TGAGCAACAG CTCCCCATCC CTCTGCCCTT CTGCTGGCTT 
TGCAGGACTC TGATCAAGCG GGTCCAAGCT GTGATTCCCA AGTGCCTGGC TGAGCGTTAC 
ACAGTACTTC TACTAGATGC ACTGCTGGGT CGTGTGGTGC CCCAGCTAGT CTGTGGCCTG 
GTCCTCCGAT GTTCCACTGC AGATGCCATT GGCCCAGCCC TCCCTGCTCT GGAGCCTCTG 
ATTGAAAAAT GGCCACTGCA AGACACTGAG TGCCATTTCT GCAAGTCTGT GATCAACCAG 
GCCTGGAACA CCAGTGAACA GGCTATGCCA CAGGCAATGC ACCAGGCCTG CCTTCGCTTC 
TGGCTAGACA GGCAAAAGTG TGAGCAGTTT GTGGAACGGC ACATGCCCCA GCTGCTGGCC 
CTGGTGCCTA GGAGCCAGGA TGCCCACACC TCCTGCCAGG CCCTTGGAGT ATGTGAGGCC 
CCGGCCAGCC CTCTGCAATG CTTCCAAACC CCACACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236719.1
CDS	47..1108
Translation	MAKLHLQWLLLLPTLCSLGAATESASSPDCAQGPKFWCQSLEQAIQCRALGHCLQEVWGHAGANDLCQECEDIVHLLTKMTKEDAFQDTIRKFLEQECDILPLKLLVPRCRQVLDVYLPLVIDYFQGQIKPKAICSHVGLCPLGQTKPEQKPEMLDAIPNPLLNKLVLPALPGAFLARPGPHTQDLSEQQLPIPLPFCWLCRTLIKRVQAVIPKCLAERYTVLLLDALLGRVVPQLVCGLVLRCSTADAIGPALPALEPLIEKWPLQDTECHFCKSVINQAWNTSEQAMPQAMHQACLRFWLDRQKCEQFVERHMPQLLALVPRSQDAHTSCQALGVCEAPASPLQCFQTPHL

Target ORF information:

RefSeq Version	XM_006236657.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus surfactant protein B (Sftpb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236657.3

ORF Insert Sequence:

ATGGCCAAGT TACATCTGCA GTGGCTACTG CTCCTTCCTA CACTCTGCAG CCTAGGCGCA 
GCTACAGAGT CGGCCTCGTC CCCGGATTGT GCACAAGGCC CTAAATTCTG GTGCCAAAGC 
CTGGAGCAAG CGATACAGTG CAGAGCCCTG GGGCACTGCC TACAGGAAGT CTGGGGACAT 
GCAGGAGCTA ATGACCTGTG CCAAGAGTGT GAGGATATTG TCCACCTCCT CACAAAGATG 
ACCAAGGAAG ACGCTTTCCA GGACACGATC CGGAAGTTCC TGGAACAAGA ATGTGATATC 
CTACCCTTGA AGCTGCTTGT GCCCCGGTGT CGCCAAGTGC TTGATGTCTA CCTGCCCCTG 
GTTATCGACT ACTTCCAGGG CCAGATTAAA CCCAAAGCCA TCTGCAGTCA TGTGGGCCTG 
TGCCCACTTG GGCAGACTAA GCCAGAGCAG AAGCCCGAGA TGCTGGATGC CATTCCAAAC 
CCTCTGCTGA ACAAGCTGGT CCTCCCTGCG CTGCCAGGAG CCTTCTTGGC AAGGCCTGGG 
CCTCACACAC AGGACCTCTC TGAGCAACAG CTCCCCATCC CTCTGCCCTT CTGCTGGCTT 
TGCAGGACTC TGATCAAGCG GGTCCAAGCT GTGATTCCCA AGTGCCTGGC TGAGCGTTAC 
ACAGTACTTC TACTAGATGC ACTGCTGGGT CGTGTGGTGC CCCAGCTAGT CTGTGGCCTG 
GTCCTCCGAT GTTCCACTGC AGATGCCATT GGCCCAGCCC TCCCTGCTCT GGAGCCTCTG 
ATTGAAAAAT GGCCACTGCA AGACACTGAG TGCCATTTCT GCAAGTCTGT GATCAACCAG 
GCCTGGAACA CCAGTGAACA GGCTATGCCA CAGGCAATGC ACCAGGCCTG CCTTCGCTTC 
TGGCTAGACA GGCAAAAGTG TGAGCAGTTT GTGGAACGGC ACATGCCCCA GCTGCTGGCC 
CTGGTGCCTA GGAGCCAGGA TGCCCACACC TCCTGCCAGG CCCTTGGAGT ATGTGAGGCC 
CCGGCCAGCC CTCTGCAATG CTTCCAAACC CCACACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A small key unlocks a heavy door: The essential function of the small hydrophobic proteins SP-B and SP-C to trigger adsorption of pulmonary surfactant lamellar bodies.
Biochimica et biophysica acta1863(8)2124-34(2016 08)
Hobi N,Giolai M,Olmeda B,Miklavc P,Felder E,Walther P,Dietl P,Frick M,P?rez-Gil J,Haller T

Activation of the GLP-1 Receptor by Liraglutide Increases ACE2 Expression, Reversing Right Ventricle Hypertrophy, and Improving the Production of SP-A and SP-B in the Lungs of Type 1 Diabetes Rats.
Endocrinology156(10)3559-69(2015 Oct)
Roman?-P?rez M,Outeiri?o-Iglesias V,Moya CM,Santisteban P,Gonz?lez-Mat?as LC,Vigo E,Mallo F

Sox9 plays multiple roles in the lung epithelium during branching morphogenesis.
Proceedings of the National Academy of Sciences of the United States of America110(47)E4456-64(2013 Nov)
Rockich BE,Hrycaj SM,Shih HP,Nagy MS,Ferguson MA,Kopp JL,Sander M,Wellik DM,Spence JR

Functional importance of the NH2-terminal insertion sequence of lung surfactant protein B.
American journal of physiology. Lung cellular and molecular physiology298(3)L335-47(2010 Mar)
Frey SL,Pocivavsek L,Waring AJ,Walther FJ,Hernandez-Juviel JM,Ruchala P,Lee KY

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Sftpb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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