Ero1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ero1a
Entrez Gene ID	171562
Full Name	endoplasmic reticulum oxidoreductase 1 alpha
Synonyms	Ero1l
General protein information	Preferred Names endoplasmic reticulum oxidoreductase 1 alpha Names ERO1-like protein alpha ERO1-L ERO1-L-alpha endoplasmic oxidoreductin-1-like protein endoplasmic reticulum oxidoreductase alpha endoplasmic reticulum oxidoreductin-1-like protein global ischemia-induced protein 11 oxidoreductin-1-L-alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p14
Summary	may function in ischemia-induced neuronal repair; may be involved in offsetting abnormalities in protein folding, distribution, or maturation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138528.1	NP_612537.1	ERO1-like protein alpha precursor

Pathways from BioSystems

Homologies

	ERO1L	XP_001103965.1
Danio rerio (zebrafish)	ero1l	NP_956644.1
Xenopus tropicalis (tropical clawed frog)	ero1lb	NP_001016058.1
Magnaporthe oryzae (rice blast fungus)	MGG_00683	XP_003718326.1
Pan troglodytes (chimpanzee)	ERO1L	XP_509950.2
Canis lupus familiaris (dog)	ERO1L	XP_547813.2
Mus musculus (house mouse)	Ero1l	NP_056589.1
Kluyveromyces lactis	KLLA0D10241g	XP_453517.1
Eremothecium gossypii	AGOS_ADL348W	NP_983747.2
Schizosaccharomyces pombe (fission yeast)	ero12	NP_588313.2
Arabidopsis thaliana (thale cress)	ERO1	NP_177372.1
Bos taurus (cattle)	ERO1L	NP_001096818.1
Gallus gallus (chicken)	ERO1L	NP_001264498.1
Saccharomyces cerevisiae (baker's yeast)	ERO1	NP_013576.1
Arabidopsis thaliana (thale cress)	ERO2	NP_973637.1
Homo sapiens (human)	ERO1L	NP_055399.1
Rattus norvegicus (Norway rat)	Ero1l	NP_612537.1
Neurospora crassa	NCU02074	XP_964601.1
Oryza sativa (rice)	Os03g0733800	NP_001051175.2

Gene Ontology
Bibliography

Related articles in PubMed

SEPN1, an endoplasmic reticulum-localized selenoprotein linked to skeletal muscle pathology, counteracts hyperoxidation by means of redox-regulating SERCA2 pump activity.
Marino M, Stoilova T, Giorgi C, Bachi A, Cattaneo A, Auricchio A, Pinton P, Zito E
Human molecular genetics24(7)1843-55(2015 Apr)

Inhibition of the functional interplay between endoplasmic reticulum (ER) oxidoreduclin-1? (Ero1?) and protein-disulfide isomerase (PDI) by the endocrine disruptor bisphenol A.
Okumura M, Kadokura H, Hashimoto S, Yutani K, Kanemura S, Hikima T, Hidaka Y, Ito L, Shiba K, Masui S, Imai D, Imaoka S, Yamaguchi H, Inaba K
The Journal of biological chemistry289(39)27004-18(2014 Sep)

Lifetime imaging of a fluorescent protein sensor reveals surprising stability of ER thiol redox.
Avezov E, Cross BC, Kaminski Schierle GS, Winters M, Harding HP, Melo EP, Kaminski CF, Ron D
The Journal of cell biology201(2)337-49(2013 Apr)

Endoplasmic reticulum thiol oxidase deficiency leads to ascorbic acid depletion and noncanonical scurvy in mice.
Zito E, Hansen HG, Yeo GS, Fujii J, Ron D
Molecular cell48(1)39-51(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that BPA, a widely distributed and potentially harmful chemical, inhibits Ero1-PDI-mediated disulfide bond formation.
Title: Inhibition of the functional interplay between endoplasmic reticulum (ER) oxidoreduclin-1? (Ero1?) and protein-disulfide isomerase (PDI) by the endocrine disruptor bisphenol A.

oxygen regulation of ERO1-Lalpha expression likely is to maintain the transfer rate of oxidizing equivalents to PDI in situations of an altered cellular redox state induced by changes of the cellular oxygen tension
Title: The cellular oxygen tension regulates expression of the endoplasmic oxidoreductase ERO1-Lalpha.

The following Ero1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ero1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12016	NM_138528.1 Latest version!	Rattus norvegicus endoplasmic reticulum oxidoreductase 1 alpha (Ero1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ero1a ( NM_138528.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_612537.1 Rattus norvegicus endoplasmic reticulum oxidoreductase 1 alpha (Ero1a), mRNA.

Price & Availability↑

CloneID	ORa12016
Clone ID Related Accession (Same CDS sequence)	NM_138528.1
Accession Version	NM_138528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	ERO1-like protein alpha precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY071924.1. On Jan 12, 2005 this sequence version replaced XM_346475.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY071924.1, BC071175.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGCCGCG CCTGGGGCTT GCTCGTCGGA CTCTTGGGCG TCGTGTGGCT GCTGCGCTTG 
GGCCACGGCG AGGAGCGGCG GCCGGAGACA GCGGCACAGC GGTGTTTCTG TCAGGTTAGT 
GGTTACCTGG ATGACTGTAC CTGTGATGTC GAGACCATCG ATAAGTTTAA TAACTACAGA 
CTTTTCCCAA GACTACAAAA GCTCCTTGAA AGTGACTACT TTAGATACTA CAAGGTAAAC 
TTGAGGAAGC CATGTCCTTT CTGGAATGAC ATCAACCAAT GTGGAAGAAG AGACTGTGCT 
GTCAAACCCT GCCATTCTGA TGAAGTCCCT GATGGAATTA AGTCTGCGAG CTACAAGTAT 
TCCAAGGAAG CCAACCTCCT TGAGGAGTGT GAGCAGGCTG AGCGGCTCGG AGCAGTGGAC 
GAATCTCTGA GTGAGGAGAC CCAGAAGGCT GTTCTTCAGT GGACGAAACA CGATGATTCT 
TCAGACAGCT TCTGTGAAGT TGATGACATA CAGTCCCCCG ATGCTGAGTA TGTGGATTTA 
CTCCTTAACC CTGAGCGCTA CACAGGCTAC AAGGGGCCGG ACGCTTGGAG GATATGGAGT 
GTCATCTATG AAGAAAACTG CTTTAAGCCA CAGACAATTC AAAGGCCTTT GGCTTCGGGG 
CAAGGAAAAC ATAAAGAGAA CACATTTTAC AGCTGGCTAG AAGGCCTCTG TGTAGAAAAG 
AGAGCATTCT ACAGGCTTAT ATCTGGCCTA CACGCAAGCA TCAATGTACA TTTGAGTGCA 
AGGTATCTTT TACAAGATAA TTGGCTGGAA AAGAAATGGG GTCATAATGT CACAGAGTTT 
CAGCAGCGCT TTGATGGGGT TTTGACAGAA GGAGAAGGCC CCAGGAGGCT GAAGAACCTG 
TACTTTCTTT ACCTGATAGA GTTAAGGGCT CTCTCTAAAG TGCTTCCGTT TTTCGAGCGC 
CCAGATTTTC AGCTCTTCAC TGGAAATAAA GTTCAGGATG TGGAAAACAA AGAGTTACTT 
CTGGAGATTC TTCATGAAGT CAAGTCATTT CCTTTGCATT TTGATGAGAA TTCTTTTTTT 
GCGGGGGATA AACACGAAGC ACATAAGCTA AAGGAGGACT TCCGCCTACA CTTTAGAAAC 
ATCTCGAGGA TCATGGACTG CGTCGGCTGC TTCAAGTGCC GCCTGTGGGG CAAGCTTCAG 
ACTCAGGGTC TGGGCACTGC TCTGAAGATC TTGTTTTCTG AAAAACTGAT CGCAAATATG 
CCGGAAAGCG GACCCAGTTA TGAATTCCAG CTAACCAGAC AAGAAATAGT GTCGTTGTTC 
AATGCATTTG GAAGGATTTC CACAAGTGTG AGAGAATTAG AGAACTTCAG ACACTTGTTA 
CAGAATGTTC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612537.1
CDS	14..1408
Translation	MGRAWGLLVGLLGVVWLLRLGHGEERRPETAAQRCFCQVSGYLDDCTCDVETIDKFNNYRLFPRLQKLLESDYFRYYKVNLRKPCPFWNDINQCGRRDCAVKPCHSDEVPDGIKSASYKYSKEANLLEECEQAERLGAVDESLSEETQKAVLQWTKHDDSSDSFCEVDDIQSPDAEYVDLLLNPERYTGYKGPDAWRIWSVIYEENCFKPQTIQRPLASGQGKHKENTFYSWLEGLCVEKRAFYRLISGLHASINVHLSARYLLQDNWLEKKWGHNVTEFQQRFDGVLTEGEGPRRLKNLYFLYLIELRALSKVLPFFERPDFQLFTGNKVQDVENKELLLEILHEVKSFPLHFDENSFFAGDKHEAHKLKEDFRLHFRNISRIMDCVGCFKCRLWGKLQTQGLGTALKILFSEKLIANMPESGPSYEFQLTRQEIVSLFNAFGRISTSVRELENFRHLLQNVH

Target ORF information:

RefSeq Version	NM_138528.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus endoplasmic reticulum oxidoreductase 1 alpha (Ero1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138528.1

ORF Insert Sequence:

ATGGGCCGCG CCTGGGGCTT GCTCGTCGGA CTCTTGGGCG TCGTGTGGCT GCTGCGCTTG 
GGCCACGGCG AGGAGCGGCG GCCGGAGACA GCGGCACAGC GGTGTTTCTG TCAGGTTAGT 
GGTTACCTGG ATGACTGTAC CTGTGATGTC GAGACCATCG ATAAGTTTAA TAACTACAGA 
CTTTTCCCAA GACTACAAAA GCTCCTTGAA AGTGACTACT TTAGATACTA CAAGGTAAAC 
TTGAGGAAGC CATGTCCTTT CTGGAATGAC ATCAACCAAT GTGGAAGAAG AGACTGTGCT 
GTCAAACCCT GCCATTCTGA TGAAGTCCCT GATGGAATTA AGTCTGCGAG CTACAAGTAT 
TCCAAGGAAG CCAACCTCCT TGAGGAGTGT GAGCAGGCTG AGCGGCTCGG AGCAGTGGAC 
GAATCTCTGA GTGAGGAGAC CCAGAAGGCT GTTCTTCAGT GGACGAAACA CGATGATTCT 
TCAGACAGCT TCTGTGAAGT TGATGACATA CAGTCCCCCG ATGCTGAGTA TGTGGATTTA 
CTCCTTAACC CTGAGCGCTA CACAGGCTAC AAGGGGCCGG ACGCTTGGAG GATATGGAGT 
GTCATCTATG AAGAAAACTG CTTTAAGCCA CAGACAATTC AAAGGCCTTT GGCTTCGGGG 
CAAGGAAAAC ATAAAGAGAA CACATTTTAC AGCTGGCTAG AAGGCCTCTG TGTAGAAAAG 
AGAGCATTCT ACAGGCTTAT ATCTGGCCTA CACGCAAGCA TCAATGTACA TTTGAGTGCA 
AGGTATCTTT TACAAGATAA TTGGCTGGAA AAGAAATGGG GTCATAATGT CACAGAGTTT 
CAGCAGCGCT TTGATGGGGT TTTGACAGAA GGAGAAGGCC CCAGGAGGCT GAAGAACCTG 
TACTTTCTTT ACCTGATAGA GTTAAGGGCT CTCTCTAAAG TGCTTCCGTT TTTCGAGCGC 
CCAGATTTTC AGCTCTTCAC TGGAAATAAA GTTCAGGATG TGGAAAACAA AGAGTTACTT 
CTGGAGATTC TTCATGAAGT CAAGTCATTT CCTTTGCATT TTGATGAGAA TTCTTTTTTT 
GCGGGGGATA AACACGAAGC ACATAAGCTA AAGGAGGACT TCCGCCTACA CTTTAGAAAC 
ATCTCGAGGA TCATGGACTG CGTCGGCTGC TTCAAGTGCC GCCTGTGGGG CAAGCTTCAG 
ACTCAGGGTC TGGGCACTGC TCTGAAGATC TTGTTTTCTG AAAAACTGAT CGCAAATATG 
CCGGAAAGCG GACCCAGTTA TGAATTCCAG CTAACCAGAC AAGAAATAGT GTCGTTGTTC 
AATGCATTTG GAAGGATTTC CACAAGTGTG AGAGAATTAG AGAACTTCAG ACACTTGTTA 
CAGAATGTTC ACTGA