Sulf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sulf1
Entrez Gene ID	171396
Full Name	sulfatase 1
General protein information	Preferred Names sulfatase 1 Names extracellular sulfatase Sulf-1 RSulfFP1 sulfatase FP
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q11
Summary	displays arylsulfatase and/or hydrolase enzyme activity; may catalyze the desulfation of glycosaminoglycans [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008763486.1	XP_008761708.1	extracellular sulfatase Sulf-1 isoform X1
XM_008763487.2	XP_008761709.1	extracellular sulfatase Sulf-1 isoform X1
NM_134378.2	NP_599205.1	extracellular sulfatase Sulf-1 precursor
XM_008763488.2	XP_008761710.1	extracellular sulfatase Sulf-1 isoform X1
XM_017593137.1	XP_017448626.1	extracellular sulfatase Sulf-1 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SULF1	XP_004939962.1
Danio rerio (zebrafish)	sulf1	NP_001003846.1
Caenorhabditis elegans (roundworm)	sul-1	NP_508560.1
Pan troglodytes (chimpanzee)	SULF1	XP_003311792.1
Homo sapiens (human)	SULF1	NP_001121677.1
Mus musculus (house mouse)	Sulf1	NP_001185494.1
Rattus norvegicus (Norway rat)	Sulf1	NP_599205.1
	SULF1	XP_002808528.1
Canis lupus familiaris (dog)	SULF1	NP_001041580.1
Xenopus tropicalis (tropical clawed frog)	sulf1	NP_001090848.1
Bos taurus (cattle)	SULF1	XP_003586945.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing.
Zaman G, Staines KA, Farquharson C, Newton PT, Dudhia J, Chenu C, Pitsillides AA, Dhoot GK
Histochemistry and cell biology145(1)67-79(2016 Jan)

Increased sulfatase 1 gene expression in degenerative intervertebral disc cells.
Tsai TT, Ho NY, Fang HC, Lai PL, Niu CC, Chen LH, Chen WJ, Pang JH
Journal of orthopaedic research : official publication of the Orthopaedic Research Society33(3)312-7(2015 Mar)

Sulf1 and Sulf2 expression in the nervous system and its role in limiting neurite outgrowth in vitro.
Joy MT, Vrbova G, Dhoot GK, Anderson PN
Experimental neurology263150-60(2015 Jan)

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
B?hm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W, Lebrecht A, Schmidt M, K?lbl H, Pfeiffer N, Grus FH
Oncology reports28(2)429-38(2012 Aug)

WT1-dependent sulfatase expression maintains the normal glomerular filtration barrier.
Schumacher VA, Schl?tzer-Schrehardt U, Karumanchi SA, Shi X, Zaia J, Jeruschke S, Zhang D, Pavenst?dt H, Pavenstaedt H, Drenckhan A, Amann K, Ng C, Hartwig S, Ng KH, Ho J, Kreidberg JA, Taglienti M, Royer-Pokora B, Ai X
Journal of the American Society of Nephrology : JASN22(7)1286-96(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Examination of epiphyseal growth plates revealed differential, inverse patterns of SULF1 and SULF2 expressions, with the former enriched in quiescent and the latter in hypertrophic chondrocyte zones.
Title: Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing.

findings show an upregulation of SULF1 in degenerative discs for the first time, and suggest that there is a link between SULF1 and disc degeneration
Title: Increased sulfatase 1 gene expression in degenerative intervertebral disc cells.

It is proposed that Sulf1, Sulf2 and ErbB1 are involved in the inhibition of neurite outgrowth and may regulate structural plasticity and regeneration in the nervous system.
Title: Sulf1 and Sulf2 expression in the nervous system and its role in limiting neurite outgrowth in vitro.

Sulf1 mRNA expression was increased in cultured vascular smooth muscle cells compared to rat aorta.
Title: Regulation of vascular smooth muscle cell proliferation, migration and death by heparan sulfate 6-O-endosulfatase1.

The following Sulf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sulf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01241	NM_134378.2 Latest version!	Rattus norvegicus sulfatase 1 (Sulf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa44313	XM_008763487.2 Latest version!	Rattus norvegicus sulfatase 1 (Sulf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa44313	XM_017593137.1 Latest version!	Rattus norvegicus sulfatase 1 (Sulf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa44313	XM_008763486.1 Latest version!	Rattus norvegicus sulfatase 1 (Sulf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa44313	XM_008763488.2 Latest version!	Rattus norvegicus sulfatase 1 (Sulf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sulf1 ( NM_134378.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_599205.1 Rattus norvegicus sulfatase 1 (Sulf1), mRNA.

Price & Availability↑

CloneID	ORa01241
Clone ID Related Accession (Same CDS sequence)	NM_134378.2
Accession Version	NM_134378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Rattus norvegicus(Norway rat)
Product	extracellular sulfatase Sulf-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF230072.1. On Aug 30, 2012 this sequence version replaced NM_134378.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF230072.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAAGAATT CCTGCTGGGC TCTGCTGCTG GCTGTGCTGG GCGCAGAACT TCTGGGAGGC 
TTCTGCTCCA CAATGCGGTC CCAGAGGTTC CGAGGACGGG TGCAGCAGGA ACGAAAAAAC 
ATCAGACCCA ATATTATCCT TGTGCTCACC GACGACCAGG ACGTGGAGTT GGGTTCCCTG 
CAAGTCATGA ACAAGACGAG AAAGATCATG GAACACGGTG GGGCCACCTT CACCAATGCC 
TTTGTGACCA CGCCCATGTG CTGCCCGTCA CGCTCCTCCA TGCTCACTGG GAAGTACGTG 
CATAACCACA ACGTCTACAC CAACAATGAG AACTGCTCTT CTCCCTCGTG GCAGGCTCTG 
CATGAGCCTC GGACCTTTGC TGTGTATCTC AACAATACTG GCTACAGAAC AGCCTTTTTT 
GGAAAATACC TCAATGAGTA TAACGGTAGC TACATCCCTC CTGGATGGCG AGAATGGCTC 
GGATTAATCA AGAATTCGCG TTTCTATAAT TACACTGTTT GTCGCAATGG CATCAAAGAG 
AAGCACGGCT TTGATTATGC AAAGGATTAC TTCACAGACT TAATCACTAA CGAGAGCATT 
AATTACTTCA AAATGTCTAA GAGAATGTAT CCCCATAGGC CTGTTATGAT GGTGATCAGC 
CACGCAGCGC CACACGGCCC TGAAGACTCT GCCCCACAGT TTTCAAAACT GTACCCTAAT 
GCCTCCCAAC ACATAACTCC GAGCTATAAC TATGCACCGA ACATGGACAA ACACTGGATC 
ATGCAGTACA CAGGTCCCAT GCTGCCTATC CACATGGAGT TCACCAACGT CCTCCAGCGC 
AAACGACTAC AGACTCTGAT GTCGGTTGAT GACTCTGTCG AGAGGCTCTA CAACATGCTT 
GTGGAGACCG GGGAACTGGG CAACACGTAC ATCATCTACA CTGCGGACCA CGGCTACCAC 
ATTGGACAGT TTGGACTGGT CAAGGGGAAA TCCATGCCCT ACGACTTTGA CATCCGCGTG 
CCTTTCTTCA TCCGTGGTCC GAGCATAGAA CCAGGGTCGA TAGTCCCACA GATTGTTCTG 
AACATTGACC TGGCCCCTAC CATTCTGGAT ATTGCCGGTC TTGACACTCC TTCTGATGTG 
GATGGCAAGT CTGTCCTGAA ACTTCTAGAC CTGGAAAAGC CAGGTAACAG GTTTCGAACA 
AACAAGAAGG CCAAAATCTG GCGTGACACA TTCCTAGTGG AAAGAGGGAA ATTTTTACGA 
AAGAAAGAGG AATCCAGCAA GAATATCCAA CAATCAAACC ACTTGCCCAA ATATGAGAGG 
GTCAAGGAAC TGTGCCAGCA GGCCAGATAC CAGACAGCCT GTGAGCAGCC TGGGCAGAAC 
TGGCAGTGCA TCGAGGACAC ATCCGGCAAG CTTCGAATCC ACAAGTGTAA GGGACCCAGC 
GACCTGCTCA CCGTCCGACA GAACGCACGC AACCTCTACT CTCGAGGATT GCAGGACAAA 
GACAAAGAGT GCCATTGTAG GGAGTCTGGT TATCGCCCGA GCAGAAGCCA GAGAAAGAAA 
GAAAGGCAGT TCCTGAGGAA CCAGGGAACA CCAAAGTATA AGCCCAGATT TGTGCACACC 
AGGCAGACTC GGTCCCTGTC GGTTGAATTC GAAGGTGAAA TATATGACAT AAATCTGGAG 
GAAGAAGAAT TGCAAGTGTT ACCGCCAAGG AGCGTTGCTA AGCGCCATGA TGAGGGCCAC 
CAGGGCTTCG GAGGCCACCA GGCTGCTGCT GGTGACTTCA GGAATGAGAT GCTGGCAGAC 
AGCAACAGTG CTGTGGGGTT ACCCACCACT GTCCGGGTGA CACACAAATG CTTTATTCTT 
CCAAATGACA CAATCCATTG TGAGAGGGAG CTGTACCAGT CGGCCAGAGC GTGGAAGGAC 
CACAAGGCCT ACATCGATAA AGAGATTGAA GTTCTACAGG ATAAAATTAA GAATTTAAGG 
GAAGTGAGGG GACACCTAAA GAAAAGGAAG CCTGAGGAGT GTAGCTGTGG TAAGCAGAGC 
TATTACAACA AAGAGAAAGG TGTCAAACGA CAGGAGAAGC TGAAGAGCCA CCTTCACCCC 
TTCAAGGAGG CTGCGGCCCA GGAGGTGGAC AGCAAACTGC AGCTGTTCAA GGAGCATCGT 
CGGAGGAAGA AGGAGAGGAA GGAGAAGAAA CGTCAGAGAA AAGGAGAAGA GTGCAGCCTG 
CCTGGCCTCA CCTGCTTCAC CCACGACAAC AACCACTGGC AGACCGCCCC GTTCTGGAAC 
TTGGGATCTT TCTGTGCATG CACGAGTTCT AACAACAATA CCTACTGGTG TTTGCGTACG 
GTCAACGAGA CGCACAATTT TCTCTTCTGT GAGTTTGCTA CTGGCTTTCT GGAGTATTTT 
GACATGAACA CAGATCCTTA CCAGCTTACA AACACGGTGC ACACGGTAGA ACGGGGCATC 
TTGAATCAGC TACACATACA GCTGATGGAG CTCCGAAGCT GCCAAGGGTA TAAACAGTGC 
AACCCAAGAC CCAAGAGCCT TGACGTTGGA ACTAAAGAAG GAGGAAACTA TGACCCACAC 
AGAGGACAGT TATGGGATGG ATGGGAAGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599205.1
CDS	413..3025
Translation	MKNSCWALLLAVLGAELLGGFCSTMRSQRFRGRVQQERKNIRPNIILVLTDDQDVELGSLQVMNKTRKIMEHGGATFTNAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSWQALHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTNVLQRKRLQTLMSVDDSVERLYNMLVETGELGNTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSIEPGSIVPQIVLNIDLAPTILDIAGLDTPSDVDGKSVLKLLDLEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARYQTACEQPGQNWQCIEDTSGKLRIHKCKGPSDLLTVRQNARNLYSRGLQDKDKECHCRESGYRPSRSQRKKERQFLRNQGTPKYKPRFVHTRQTRSLSVEFEGEIYDINLEEEELQVLPPRSVAKRHDEGHQGFGGHQAAAGDFRNEMLADSNSAVGLPTTVRVTHKCFILPNDTIHCERELYQSARAWKDHKAYIDKEIEVLQDKIKNLREVRGHLKKRKPEECSCGKQSYYNKEKGVKRQEKLKSHLHPFKEAAAQEVDSKLQLFKEHRRRKKERKEKKRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETHNFLFCEFATGFLEYFDMNTDPYQLTNTVHTVERGILNQLHIQLMELRSCQGYKQCNPRPKSLDVGTKEGGNYDPHRGQLWDGWEG

Target ORF information:

RefSeq Version	NM_134378.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sulfatase 1 (Sulf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134378.2

ORF Insert Sequence:

ATGAAGAATT CCTGCTGGGC TCTGCTGCTG GCTGTGCTGG GCGCAGAACT TCTGGGAGGC 
TTCTGCTCCA CAATGCGGTC CCAGAGGTTC CGAGGACGGG TGCAGCAGGA ACGAAAAAAC 
ATCAGACCCA ATATTATCCT TGTGCTCACC GACGACCAGG ACGTGGAGTT GGGTTCCCTG 
CAAGTCATGA ACAAGACGAG AAAGATCATG GAACACGGTG GGGCCACCTT CACCAATGCC 
TTTGTGACCA CGCCCATGTG CTGCCCGTCA CGCTCCTCCA TGCTCACTGG GAAGTACGTG 
CATAACCACA ACGTCTACAC CAACAATGAG AACTGCTCTT CTCCCTCGTG GCAGGCTCTG 
CATGAGCCTC GGACCTTTGC TGTGTATCTC AACAATACTG GCTACAGAAC AGCCTTTTTT 
GGAAAATACC TCAATGAGTA TAACGGTAGC TACATCCCTC CTGGATGGCG AGAATGGCTC 
GGATTAATCA AGAATTCGCG TTTCTATAAT TACACTGTTT GTCGCAATGG CATCAAAGAG 
AAGCACGGCT TTGATTATGC AAAGGATTAC TTCACAGACT TAATCACTAA CGAGAGCATT 
AATTACTTCA AAATGTCTAA GAGAATGTAT CCCCATAGGC CTGTTATGAT GGTGATCAGC 
CACGCAGCGC CACACGGCCC TGAAGACTCT GCCCCACAGT TTTCAAAACT GTACCCTAAT 
GCCTCCCAAC ACATAACTCC GAGCTATAAC TATGCACCGA ACATGGACAA ACACTGGATC 
ATGCAGTACA CAGGTCCCAT GCTGCCTATC CACATGGAGT TCACCAACGT CCTCCAGCGC 
AAACGACTAC AGACTCTGAT GTCGGTTGAT GACTCTGTCG AGAGGCTCTA CAACATGCTT 
GTGGAGACCG GGGAACTGGG CAACACGTAC ATCATCTACA CTGCGGACCA CGGCTACCAC 
ATTGGACAGT TTGGACTGGT CAAGGGGAAA TCCATGCCCT ACGACTTTGA CATCCGCGTG 
CCTTTCTTCA TCCGTGGTCC GAGCATAGAA CCAGGGTCGA TAGTCCCACA GATTGTTCTG 
AACATTGACC TGGCCCCTAC CATTCTGGAT ATTGCCGGTC TTGACACTCC TTCTGATGTG 
GATGGCAAGT CTGTCCTGAA ACTTCTAGAC CTGGAAAAGC CAGGTAACAG GTTTCGAACA 
AACAAGAAGG CCAAAATCTG GCGTGACACA TTCCTAGTGG AAAGAGGGAA ATTTTTACGA 
AAGAAAGAGG AATCCAGCAA GAATATCCAA CAATCAAACC ACTTGCCCAA ATATGAGAGG 
GTCAAGGAAC TGTGCCAGCA GGCCAGATAC CAGACAGCCT GTGAGCAGCC TGGGCAGAAC 
TGGCAGTGCA TCGAGGACAC ATCCGGCAAG CTTCGAATCC ACAAGTGTAA GGGACCCAGC 
GACCTGCTCA CCGTCCGACA GAACGCACGC AACCTCTACT CTCGAGGATT GCAGGACAAA 
GACAAAGAGT GCCATTGTAG GGAGTCTGGT TATCGCCCGA GCAGAAGCCA GAGAAAGAAA 
GAAAGGCAGT TCCTGAGGAA CCAGGGAACA CCAAAGTATA AGCCCAGATT TGTGCACACC 
AGGCAGACTC GGTCCCTGTC GGTTGAATTC GAAGGTGAAA TATATGACAT AAATCTGGAG 
GAAGAAGAAT TGCAAGTGTT ACCGCCAAGG AGCGTTGCTA AGCGCCATGA TGAGGGCCAC 
CAGGGCTTCG GAGGCCACCA GGCTGCTGCT GGTGACTTCA GGAATGAGAT GCTGGCAGAC 
AGCAACAGTG CTGTGGGGTT ACCCACCACT GTCCGGGTGA CACACAAATG CTTTATTCTT 
CCAAATGACA CAATCCATTG TGAGAGGGAG CTGTACCAGT CGGCCAGAGC GTGGAAGGAC 
CACAAGGCCT ACATCGATAA AGAGATTGAA GTTCTACAGG ATAAAATTAA GAATTTAAGG 
GAAGTGAGGG GACACCTAAA GAAAAGGAAG CCTGAGGAGT GTAGCTGTGG TAAGCAGAGC 
TATTACAACA AAGAGAAAGG TGTCAAACGA CAGGAGAAGC TGAAGAGCCA CCTTCACCCC 
TTCAAGGAGG CTGCGGCCCA GGAGGTGGAC AGCAAACTGC AGCTGTTCAA GGAGCATCGT 
CGGAGGAAGA AGGAGAGGAA GGAGAAGAAA CGTCAGAGAA AAGGAGAAGA GTGCAGCCTG 
CCTGGCCTCA CCTGCTTCAC CCACGACAAC AACCACTGGC AGACCGCCCC GTTCTGGAAC 
TTGGGATCTT TCTGTGCATG CACGAGTTCT AACAACAATA CCTACTGGTG TTTGCGTACG 
GTCAACGAGA CGCACAATTT TCTCTTCTGT GAGTTTGCTA CTGGCTTTCT GGAGTATTTT 
GACATGAACA CAGATCCTTA CCAGCTTACA AACACGGTGC ACACGGTAGA ACGGGGCATC 
TTGAATCAGC TACACATACA GCTGATGGAG CTCCGAAGCT GCCAAGGGTA TAAACAGTGC 
AACCCAAGAC CCAAGAGCCT TGACGTTGGA ACTAAAGAAG GAGGAAACTA TGACCCACAC 
AGAGGACAGT TATGGGATGG ATGGGAAGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sulf1 ( XM_008763486.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761708.1 Rattus norvegicus sulfatase 1 (Sulf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa44313
Clone ID Related Accession (Same CDS sequence)	XM_008763486.1 , XM_008763487.2 , XM_008763488.2 , XM_017593137.1
Accession Version	XM_008763486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	extracellular sulfatase Sulf-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAATT CCTGCTGGGC TCTGCTGCTG GCTGTGCTGG GCGCAGAACT TCTGGGAGGC 
TTCTGCTCCA CAATGCGGTC CCAGAGGTTC CGAGGACGGG TGCAGCAGGA ACGAAAAAAC 
ATCAGACCCA ATATTATCCT TGTGCTCACC GACGACCAGG ACGTGGAGTT GGGTTCCCTG 
CAAGTCATGA ACAAGACGAG AAAGATCATG GAACACGGTG GGGCCACCTT CACCAATGCC 
TTTGTGACCA CGCCCATGTG CTGCCCGTCA CGCTCCTCCA TGCTCACTGG GAAGTACGTG 
CATAACCACA ACGTCTACAC CAACAATGAG AACTGCTCTT CTCCCTCGTG GCAGGCTCTG 
CATGAGCCTC GGACCTTTGC TGTGTATCTC AACAATACTG GCTACAGAAC AGCCTTTTTT 
GGAAAATACC TCAATGAGTA TAACGGTAGC TACATCCCTC CTGGATGGCG AGAATGGCTC 
GGATTAATCA AGAATTCGCG TTTCTATAAT TACACTGTTT GTCGCAATGG CATCAAAGAG 
AAGCACGGCT TTGATTATGC AAAGGATTAC TTCACAGACT TAATCACTAA CGAGAGCATT 
AATTACTTCA AAATGTCTAA GAGAATGTAT CCCCATAGGC CTGTTATGAT GGTGATCAGC 
CACGCAGCGC CACACGGCCC TGAAGACTCT GCCCCACAGT TTTCAAAACT GTACCCTAAT 
GCCTCCCAAC ACATAACTCC GAGCTATAAC TATGCACCGA ACATGGACAA ACACTGGATC 
ATGCAGTACA CAGGTCCCAT GCTGCCTATC CACATGGAGT TCACCAACGT CCTCCAGCGC 
AAACGACTAC AGACTCTGAT GTCGGTTGAT GACTCTGTCG AGAGGCTCTA CAACATGCTT 
GTGGAGACCG GGGAACTGGG CAACACGTAC ATCATCTACA CTGCGGACCA CGGCTACCAC 
ATTGGACAGT TTGGACTGGT CAAGGGGAAA TCCATGCCCT ACGACTTTGA CATCCGCGTG 
CCTTTCTTCA TCCGTGGTCC GAGCATAGAA CCAGGGTCCA TAGTCCCACA GATTGTTCTG 
AACATTGACC TGGCCCCTAC CATTCTGGAT ATCGCCGGTC TTGACACTCC TTCTGATGTG 
GATGGCAAGT CTGTCCTGAA ACTTCTAGAC CTGGAAAAGC CGGGTAACAG GTTTCGAACA 
AACAAGAAGG CCAAAATCTG GCGTGACACA TTCCTAGTGG AAAGAGGGAA ATTTTTACGA 
AAGAAGGAGG AATCCAGCAA GAATATCCAA CAATCAAACC ACTTGCCCAA ATATGAGAGG 
GTCAAGGAAC TGTGCCAGCA GGCCAGATAC CAGACAGCCT GTGAGCAGCC TGGGCAGAAC 
TGGCAGTGCA TCGAGGACAC ATCCGGCAAG CTTCGAATCC ACAAGTGTAA GGGACCCAGC 
GACCTGCTCA CCGTCCGACA GAACGCACGC AACCTCTACT CTCGAGGATT GCAGGACAAA 
GACAAAGAGT GCCATTGTAG GGAGTCTGGT TATCGCCCGA GCAGAAGCCA GAGAAAGAAA 
GAAAGGCAGT TCCTGAGGAA CCAGGGAACA CCAAAGTATA AGCCCAGATT TGTGCACACC 
AGGCAGACTC GGTCCCTGTC GGTTGAATTC GAAGGTGAAA TATATGACAT AAATCTGGAG 
GAAGAAGAAT TGCAAGTGTT ACCGCCAAGG AGCGTTGCTA AGCGCCATGA TGAGGGCCAC 
CAGGGCTTCG GAGGCCACCA GGCTGCTGCT GGTGACTTCA GGAATGAGAT GCTGGCAGAC 
AGCAACAGTG CTGTGGGGTT ACCCACCACT GTCCGGGTGA CACACAAATG CTTTATTCTT 
CCAAATGACA CAATCCATTG TGAGAGGGAG CTGTACCAGT CGGCCAGAGC GTGGAAGGAC 
CACAAGGCCT ACATCGATAA AGAGATTGAA GTTCTACAGG ATAAAATTAA GAATTTAAGG 
GAAGTGAGGG GACACCTAAA GAAAAGGAAG CCTGAGGAGT GTAGCTGTGG TAAGCAGAGC 
TATTACAACA AAGAGAAAGG TGTCAAACGA CAGGAGAAGC TGAAGAGCCA CCTTCACCCC 
TTCAAGGAGG CTGCGGCCCA GGAGGTGGAC AGCAAACTGC AGCTGTTCAA GGAGCATCGT 
CGGAGGAAGA AGGAGAGGAA GGAGAAGAAA CGTCAGAGAA AAGGAGAAGA GTGCAGCCTG 
CCTGGCCTCA CCTGCTTCAC CCACGACAAC AACCACTGGC AGACCGCCCC GTTCTGGAAC 
TTGGGATCTT TCTGTGCATG CACGAGTTCT AACAACAATA CCTACTGGTG TTTGCGTACG 
GTCAATGAGA CGCACAATTT TCTCTTTTGT GAGTTTGCTA CTGGCTTTCT GGAGTATTTT 
GACATGAACA CAGATCCTTA CCAGCTTACA AACACGGTGC ACACGATAGA ACGGGGCATC 
TTGAATCAGC TACACATACA GCTGATGGAG CTCCGAAGCT GCCAAGGGTA TAAACAGTGC 
AACCCAAGAC CCAAGAGCCT TGACGTTGAG GACAGTTATG GGATGGATGG GAAGGTTAGT 
TGGTCCAGTG TCGCTTCAGA CACCAACTGG CAAGGCCTGG AGGAGTTATC TGGTGCAAAC 
GACATCAAAG AGGACAGATC TAACCCTAGA CTGAGGCCGG AGGCCTGGAC CAATTACCTG 
AGGGATGTCC ACAGAGCCTT TGCACTGCTG AACAGTCACC CTGATCCAAA CCAAGTAAAT 
GGGACTCCAA CTGCACCAAG CGGTGGCCTC CCAGTCACCT CTGCTGAGCT CTTGGTGCCG 
GCAGAGATGG CTTCTGCAGA GTCAGGTGAA GACCCAAGTC ATGTGGTTGG GGAAACGCCT 
CCTTTGACCT TGCCAGCCAA CCTCCAAACC CTGCATCCGA ACAGACCAAC GTTGAGTCCA 
GAGAGAAAAC TTGAATGGAA TAACGACATT CCAGAAGTGA ATCGTTTGAA TTCTGAACAC 
TGGAGAAAAA CTGAGGAGCA GCCAGGACGG GGGGAGGTGC TTCTCCCCGA AGGTGACGTC 
AGTGGCAACG GTATGACAGA GCTGTTGCCC ATCGGTCGGC ACCAACAAAA GCGTCCCCAC 
GATGCGGGGC CAGAGGACCA TGCTTTTGAA GATCAATTGC ATCCTCTCGT CCACTCTGAC 
AGAACTCCCG TTCATCGGGT GTTCGATGTG TCCCACTTGG AGCAGCCTGT TCACTCCAGC 
CACGTGGAAG GAATGTTGGC CAAGATGGAG GGGATGGCAC AAAGGAGTGG GCACCAAGTC 
TCGAAGGCAG CGCCTCCTCT CCAGTCACTT CTGCTTAGAT TACATGTTGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761708.1
CDS	985..4338
Translation	MKNSCWALLLAVLGAELLGGFCSTMRSQRFRGRVQQERKNIRPNIILVLTDDQDVELGSLQVMNKTRKIMEHGGATFTNAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSWQALHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTNVLQRKRLQTLMSVDDSVERLYNMLVETGELGNTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSIEPGSIVPQIVLNIDLAPTILDIAGLDTPSDVDGKSVLKLLDLEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARYQTACEQPGQNWQCIEDTSGKLRIHKCKGPSDLLTVRQNARNLYSRGLQDKDKECHCRESGYRPSRSQRKKERQFLRNQGTPKYKPRFVHTRQTRSLSVEFEGEIYDINLEEEELQVLPPRSVAKRHDEGHQGFGGHQAAAGDFRNEMLADSNSAVGLPTTVRVTHKCFILPNDTIHCERELYQSARAWKDHKAYIDKEIEVLQDKIKNLREVRGHLKKRKPEECSCGKQSYYNKEKGVKRQEKLKSHLHPFKEAAAQEVDSKLQLFKEHRRRKKERKEKKRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETHNFLFCEFATGFLEYFDMNTDPYQLTNTVHTIERGILNQLHIQLMELRSCQGYKQCNPRPKSLDVEDSYGMDGKVSWSSVASDTNWQGLEELSGANDIKEDRSNPRLRPEAWTNYLRDVHRAFALLNSHPDPNQVNGTPTAPSGGLPVTSAELLVPAEMASAESGEDPSHVVGETPPLTLPANLQTLHPNRPTLSPERKLEWNNDIPEVNRLNSEHWRKTEEQPGRGEVLLPEGDVSGNGMTELLPIGRHQQKRPHDAGPEDHAFEDQLHPLVHSDRTPVHRVFDVSHLEQPVHSSHVEGMLAKMEGMAQRSGHQVSKAAPPLQSLLLRLHVA

Target ORF information:

RefSeq Version	XM_008763486.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sulfatase 1 (Sulf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763486.1

ORF Insert Sequence:

ATGAAGAATT CCTGCTGGGC TCTGCTGCTG GCTGTGCTGG GCGCAGAACT TCTGGGAGGC 
TTCTGCTCCA CAATGCGGTC CCAGAGGTTC CGAGGACGGG TGCAGCAGGA ACGAAAAAAC 
ATCAGACCCA ATATTATCCT TGTGCTCACC GACGACCAGG ACGTGGAGTT GGGTTCCCTG 
CAAGTCATGA ACAAGACGAG AAAGATCATG GAACACGGTG GGGCCACCTT CACCAATGCC 
TTTGTGACCA CGCCCATGTG CTGCCCGTCA CGCTCCTCCA TGCTCACTGG GAAGTACGTG 
CATAACCACA ACGTCTACAC CAACAATGAG AACTGCTCTT CTCCCTCGTG GCAGGCTCTG 
CATGAGCCTC GGACCTTTGC TGTGTATCTC AACAATACTG GCTACAGAAC AGCCTTTTTT 
GGAAAATACC TCAATGAGTA TAACGGTAGC TACATCCCTC CTGGATGGCG AGAATGGCTC 
GGATTAATCA AGAATTCGCG TTTCTATAAT TACACTGTTT GTCGCAATGG CATCAAAGAG 
AAGCACGGCT TTGATTATGC AAAGGATTAC TTCACAGACT TAATCACTAA CGAGAGCATT 
AATTACTTCA AAATGTCTAA GAGAATGTAT CCCCATAGGC CTGTTATGAT GGTGATCAGC 
CACGCAGCGC CACACGGCCC TGAAGACTCT GCCCCACAGT TTTCAAAACT GTACCCTAAT 
GCCTCCCAAC ACATAACTCC GAGCTATAAC TATGCACCGA ACATGGACAA ACACTGGATC 
ATGCAGTACA CAGGTCCCAT GCTGCCTATC CACATGGAGT TCACCAACGT CCTCCAGCGC 
AAACGACTAC AGACTCTGAT GTCGGTTGAT GACTCTGTCG AGAGGCTCTA CAACATGCTT 
GTGGAGACCG GGGAACTGGG CAACACGTAC ATCATCTACA CTGCGGACCA CGGCTACCAC 
ATTGGACAGT TTGGACTGGT CAAGGGGAAA TCCATGCCCT ACGACTTTGA CATCCGCGTG 
CCTTTCTTCA TCCGTGGTCC GAGCATAGAA CCAGGGTCCA TAGTCCCACA GATTGTTCTG 
AACATTGACC TGGCCCCTAC CATTCTGGAT ATCGCCGGTC TTGACACTCC TTCTGATGTG 
GATGGCAAGT CTGTCCTGAA ACTTCTAGAC CTGGAAAAGC CGGGTAACAG GTTTCGAACA 
AACAAGAAGG CCAAAATCTG GCGTGACACA TTCCTAGTGG AAAGAGGGAA ATTTTTACGA 
AAGAAGGAGG AATCCAGCAA GAATATCCAA CAATCAAACC ACTTGCCCAA ATATGAGAGG 
GTCAAGGAAC TGTGCCAGCA GGCCAGATAC CAGACAGCCT GTGAGCAGCC TGGGCAGAAC 
TGGCAGTGCA TCGAGGACAC ATCCGGCAAG CTTCGAATCC ACAAGTGTAA GGGACCCAGC 
GACCTGCTCA CCGTCCGACA GAACGCACGC AACCTCTACT CTCGAGGATT GCAGGACAAA 
GACAAAGAGT GCCATTGTAG GGAGTCTGGT TATCGCCCGA GCAGAAGCCA GAGAAAGAAA 
GAAAGGCAGT TCCTGAGGAA CCAGGGAACA CCAAAGTATA AGCCCAGATT TGTGCACACC 
AGGCAGACTC GGTCCCTGTC GGTTGAATTC GAAGGTGAAA TATATGACAT AAATCTGGAG 
GAAGAAGAAT TGCAAGTGTT ACCGCCAAGG AGCGTTGCTA AGCGCCATGA TGAGGGCCAC 
CAGGGCTTCG GAGGCCACCA GGCTGCTGCT GGTGACTTCA GGAATGAGAT GCTGGCAGAC 
AGCAACAGTG CTGTGGGGTT ACCCACCACT GTCCGGGTGA CACACAAATG CTTTATTCTT 
CCAAATGACA CAATCCATTG TGAGAGGGAG CTGTACCAGT CGGCCAGAGC GTGGAAGGAC 
CACAAGGCCT ACATCGATAA AGAGATTGAA GTTCTACAGG ATAAAATTAA GAATTTAAGG 
GAAGTGAGGG GACACCTAAA GAAAAGGAAG CCTGAGGAGT GTAGCTGTGG TAAGCAGAGC 
TATTACAACA AAGAGAAAGG TGTCAAACGA CAGGAGAAGC TGAAGAGCCA CCTTCACCCC 
TTCAAGGAGG CTGCGGCCCA GGAGGTGGAC AGCAAACTGC AGCTGTTCAA GGAGCATCGT 
CGGAGGAAGA AGGAGAGGAA GGAGAAGAAA CGTCAGAGAA AAGGAGAAGA GTGCAGCCTG 
CCTGGCCTCA CCTGCTTCAC CCACGACAAC AACCACTGGC AGACCGCCCC GTTCTGGAAC 
TTGGGATCTT TCTGTGCATG CACGAGTTCT AACAACAATA CCTACTGGTG TTTGCGTACG 
GTCAATGAGA CGCACAATTT TCTCTTTTGT GAGTTTGCTA CTGGCTTTCT GGAGTATTTT 
GACATGAACA CAGATCCTTA CCAGCTTACA AACACGGTGC ACACGATAGA ACGGGGCATC 
TTGAATCAGC TACACATACA GCTGATGGAG CTCCGAAGCT GCCAAGGGTA TAAACAGTGC 
AACCCAAGAC CCAAGAGCCT TGACGTTGAG GACAGTTATG GGATGGATGG GAAGGTTAGT 
TGGTCCAGTG TCGCTTCAGA CACCAACTGG CAAGGCCTGG AGGAGTTATC TGGTGCAAAC 
GACATCAAAG AGGACAGATC TAACCCTAGA CTGAGGCCGG AGGCCTGGAC CAATTACCTG 
AGGGATGTCC ACAGAGCCTT TGCACTGCTG AACAGTCACC CTGATCCAAA CCAAGTAAAT 
GGGACTCCAA CTGCACCAAG CGGTGGCCTC CCAGTCACCT CTGCTGAGCT CTTGGTGCCG 
GCAGAGATGG CTTCTGCAGA GTCAGGTGAA GACCCAAGTC ATGTGGTTGG GGAAACGCCT 
CCTTTGACCT TGCCAGCCAA CCTCCAAACC CTGCATCCGA ACAGACCAAC GTTGAGTCCA 
GAGAGAAAAC TTGAATGGAA TAACGACATT CCAGAAGTGA ATCGTTTGAA TTCTGAACAC 
TGGAGAAAAA CTGAGGAGCA GCCAGGACGG GGGGAGGTGC TTCTCCCCGA AGGTGACGTC 
AGTGGCAACG GTATGACAGA GCTGTTGCCC ATCGGTCGGC ACCAACAAAA GCGTCCCCAC 
GATGCGGGGC CAGAGGACCA TGCTTTTGAA GATCAATTGC ATCCTCTCGT CCACTCTGAC 
AGAACTCCCG TTCATCGGGT GTTCGATGTG TCCCACTTGG AGCAGCCTGT TCACTCCAGC 
CACGTGGAAG GAATGTTGGC CAAGATGGAG GGGATGGCAC AAAGGAGTGG GCACCAAGTC 
TCGAAGGCAG CGCCTCCTCT CCAGTCACTT CTGCTTAGAT TACATGTTGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sulf1 ( XM_008763487.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761709.1 Rattus norvegicus sulfatase 1 (Sulf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa44313
Clone ID Related Accession (Same CDS sequence)	XM_008763486.1 , XM_008763487.2 , XM_008763488.2 , XM_017593137.1
Accession Version	XM_008763487.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	extracellular sulfatase Sulf-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAATT CCTGCTGGGC TCTGCTGCTG GCTGTGCTGG GCGCAGAACT TCTGGGAGGC 
TTCTGCTCCA CAATGCGGTC CCAGAGGTTC CGAGGACGGG TGCAGCAGGA ACGAAAAAAC 
ATCAGACCCA ATATTATCCT TGTGCTCACC GACGACCAGG ACGTGGAGTT GGGTTCCCTG 
CAAGTCATGA ACAAGACGAG AAAGATCATG GAACACGGTG GGGCCACCTT CACCAATGCC 
TTTGTGACCA CGCCCATGTG CTGCCCGTCA CGCTCCTCCA TGCTCACTGG GAAGTACGTG 
CATAACCACA ACGTCTACAC CAACAATGAG AACTGCTCTT CTCCCTCGTG GCAGGCTCTG 
CATGAGCCTC GGACCTTTGC TGTGTATCTC AACAATACTG GCTACAGAAC AGCCTTTTTT 
GGAAAATACC TCAATGAGTA TAACGGTAGC TACATCCCTC CTGGATGGCG AGAATGGCTC 
GGATTAATCA AGAATTCGCG TTTCTATAAT TACACTGTTT GTCGCAATGG CATCAAAGAG 
AAGCACGGCT TTGATTATGC AAAGGATTAC TTCACAGACT TAATCACTAA CGAGAGCATT 
AATTACTTCA AAATGTCTAA GAGAATGTAT CCCCATAGGC CTGTTATGAT GGTGATCAGC 
CACGCAGCGC CACACGGCCC TGAAGACTCT GCCCCACAGT TTTCAAAACT GTACCCTAAT 
GCCTCCCAAC ACATAACTCC GAGCTATAAC TATGCACCGA ACATGGACAA ACACTGGATC 
ATGCAGTACA CAGGTCCCAT GCTGCCTATC CACATGGAGT TCACCAACGT CCTCCAGCGC 
AAACGACTAC AGACTCTGAT GTCGGTTGAT GACTCTGTCG AGAGGCTCTA CAACATGCTT 
GTGGAGACCG GGGAACTGGG CAACACGTAC ATCATCTACA CTGCGGACCA CGGCTACCAC 
ATTGGACAGT TTGGACTGGT CAAGGGGAAA TCCATGCCCT ACGACTTTGA CATCCGCGTG 
CCTTTCTTCA TCCGTGGTCC GAGCATAGAA CCAGGGTCCA TAGTCCCACA GATTGTTCTG 
AACATTGACC TGGCCCCTAC CATTCTGGAT ATCGCCGGTC TTGACACTCC TTCTGATGTG 
GATGGCAAGT CTGTCCTGAA ACTTCTAGAC CTGGAAAAGC CGGGTAACAG GTTTCGAACA 
AACAAGAAGG CCAAAATCTG GCGTGACACA TTCCTAGTGG AAAGAGGGAA ATTTTTACGA 
AAGAAGGAGG AATCCAGCAA GAATATCCAA CAATCAAACC ACTTGCCCAA ATATGAGAGG 
GTCAAGGAAC TGTGCCAGCA GGCCAGATAC CAGACAGCCT GTGAGCAGCC TGGGCAGAAC 
TGGCAGTGCA TCGAGGACAC ATCCGGCAAG CTTCGAATCC ACAAGTGTAA GGGACCCAGC 
GACCTGCTCA CCGTCCGACA GAACGCACGC AACCTCTACT CTCGAGGATT GCAGGACAAA 
GACAAAGAGT GCCATTGTAG GGAGTCTGGT TATCGCCCGA GCAGAAGCCA GAGAAAGAAA 
GAAAGGCAGT TCCTGAGGAA CCAGGGAACA CCAAAGTATA AGCCCAGATT TGTGCACACC 
AGGCAGACTC GGTCCCTGTC GGTTGAATTC GAAGGTGAAA TATATGACAT AAATCTGGAG 
GAAGAAGAAT TGCAAGTGTT ACCGCCAAGG AGCGTTGCTA AGCGCCATGA TGAGGGCCAC 
CAGGGCTTCG GAGGCCACCA GGCTGCTGCT GGTGACTTCA GGAATGAGAT GCTGGCAGAC 
AGCAACAGTG CTGTGGGGTT ACCCACCACT GTCCGGGTGA CACACAAATG CTTTATTCTT 
CCAAATGACA CAATCCATTG TGAGAGGGAG CTGTACCAGT CGGCCAGAGC GTGGAAGGAC 
CACAAGGCCT ACATCGATAA AGAGATTGAA GTTCTACAGG ATAAAATTAA GAATTTAAGG 
GAAGTGAGGG GACACCTAAA GAAAAGGAAG CCTGAGGAGT GTAGCTGTGG TAAGCAGAGC 
TATTACAACA AAGAGAAAGG TGTCAAACGA CAGGAGAAGC TGAAGAGCCA CCTTCACCCC 
TTCAAGGAGG CTGCGGCCCA GGAGGTGGAC AGCAAACTGC AGCTGTTCAA GGAGCATCGT 
CGGAGGAAGA AGGAGAGGAA GGAGAAGAAA CGTCAGAGAA AAGGAGAAGA GTGCAGCCTG 
CCTGGCCTCA CCTGCTTCAC CCACGACAAC AACCACTGGC AGACCGCCCC GTTCTGGAAC 
TTGGGATCTT TCTGTGCATG CACGAGTTCT AACAACAATA CCTACTGGTG TTTGCGTACG 
GTCAATGAGA CGCACAATTT TCTCTTTTGT GAGTTTGCTA CTGGCTTTCT GGAGTATTTT 
GACATGAACA CAGATCCTTA CCAGCTTACA AACACGGTGC ACACGATAGA ACGGGGCATC 
TTGAATCAGC TACACATACA GCTGATGGAG CTCCGAAGCT GCCAAGGGTA TAAACAGTGC 
AACCCAAGAC CCAAGAGCCT TGACGTTGAG GACAGTTATG GGATGGATGG GAAGGTTAGT 
TGGTCCAGTG TCGCTTCAGA CACCAACTGG CAAGGCCTGG AGGAGTTATC TGGTGCAAAC 
GACATCAAAG AGGACAGATC TAACCCTAGA CTGAGGCCGG AGGCCTGGAC CAATTACCTG 
AGGGATGTCC ACAGAGCCTT TGCACTGCTG AACAGTCACC CTGATCCAAA CCAAGTAAAT 
GGGACTCCAA CTGCACCAAG CGGTGGCCTC CCAGTCACCT CTGCTGAGCT CTTGGTGCCG 
GCAGAGATGG CTTCTGCAGA GTCAGGTGAA GACCCAAGTC ATGTGGTTGG GGAAACGCCT 
CCTTTGACCT TGCCAGCCAA CCTCCAAACC CTGCATCCGA ACAGACCAAC GTTGAGTCCA 
GAGAGAAAAC TTGAATGGAA TAACGACATT CCAGAAGTGA ATCGTTTGAA TTCTGAACAC 
TGGAGAAAAA CTGAGGAGCA GCCAGGACGG GGGGAGGTGC TTCTCCCCGA AGGTGACGTC 
AGTGGCAACG GTATGACAGA GCTGTTGCCC ATCGGTCGGC ACCAACAAAA GCGTCCCCAC 
GATGCGGGGC CAGAGGACCA TGCTTTTGAA GATCAATTGC ATCCTCTCGT CCACTCTGAC 
AGAACTCCCG TTCATCGGGT GTTCGATGTG TCCCACTTGG AGCAGCCTGT TCACTCCAGC 
CACGTGGAAG GAATGTTGGC CAAGATGGAG GGGATGGCAC AAAGGAGTGG GCACCAAGTC 
TCGAAGGCAG CGCCTCCTCT CCAGTCACTT CTGCTTAGAT TACATGTTGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761709.1
CDS	1134..4487
Translation	MKNSCWALLLAVLGAELLGGFCSTMRSQRFRGRVQQERKNIRPNIILVLTDDQDVELGSLQVMNKTRKIMEHGGATFTNAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSWQALHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTNVLQRKRLQTLMSVDDSVERLYNMLVETGELGNTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSIEPGSIVPQIVLNIDLAPTILDIAGLDTPSDVDGKSVLKLLDLEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARYQTACEQPGQNWQCIEDTSGKLRIHKCKGPSDLLTVRQNARNLYSRGLQDKDKECHCRESGYRPSRSQRKKERQFLRNQGTPKYKPRFVHTRQTRSLSVEFEGEIYDINLEEEELQVLPPRSVAKRHDEGHQGFGGHQAAAGDFRNEMLADSNSAVGLPTTVRVTHKCFILPNDTIHCERELYQSARAWKDHKAYIDKEIEVLQDKIKNLREVRGHLKKRKPEECSCGKQSYYNKEKGVKRQEKLKSHLHPFKEAAAQEVDSKLQLFKEHRRRKKERKEKKRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETHNFLFCEFATGFLEYFDMNTDPYQLTNTVHTIERGILNQLHIQLMELRSCQGYKQCNPRPKSLDVEDSYGMDGKVSWSSVASDTNWQGLEELSGANDIKEDRSNPRLRPEAWTNYLRDVHRAFALLNSHPDPNQVNGTPTAPSGGLPVTSAELLVPAEMASAESGEDPSHVVGETPPLTLPANLQTLHPNRPTLSPERKLEWNNDIPEVNRLNSEHWRKTEEQPGRGEVLLPEGDVSGNGMTELLPIGRHQQKRPHDAGPEDHAFEDQLHPLVHSDRTPVHRVFDVSHLEQPVHSSHVEGMLAKMEGMAQRSGHQVSKAAPPLQSLLLRLHVA

Target ORF information:

RefSeq Version	XM_008763487.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sulfatase 1 (Sulf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763487.2

ORF Insert Sequence:

ATGAAGAATT CCTGCTGGGC TCTGCTGCTG GCTGTGCTGG GCGCAGAACT TCTGGGAGGC 
TTCTGCTCCA CAATGCGGTC CCAGAGGTTC CGAGGACGGG TGCAGCAGGA ACGAAAAAAC 
ATCAGACCCA ATATTATCCT TGTGCTCACC GACGACCAGG ACGTGGAGTT GGGTTCCCTG 
CAAGTCATGA ACAAGACGAG AAAGATCATG GAACACGGTG GGGCCACCTT CACCAATGCC 
TTTGTGACCA CGCCCATGTG CTGCCCGTCA CGCTCCTCCA TGCTCACTGG GAAGTACGTG 
CATAACCACA ACGTCTACAC CAACAATGAG AACTGCTCTT CTCCCTCGTG GCAGGCTCTG 
CATGAGCCTC GGACCTTTGC TGTGTATCTC AACAATACTG GCTACAGAAC AGCCTTTTTT 
GGAAAATACC TCAATGAGTA TAACGGTAGC TACATCCCTC CTGGATGGCG AGAATGGCTC 
GGATTAATCA AGAATTCGCG TTTCTATAAT TACACTGTTT GTCGCAATGG CATCAAAGAG 
AAGCACGGCT TTGATTATGC AAAGGATTAC TTCACAGACT TAATCACTAA CGAGAGCATT 
AATTACTTCA AAATGTCTAA GAGAATGTAT CCCCATAGGC CTGTTATGAT GGTGATCAGC 
CACGCAGCGC CACACGGCCC TGAAGACTCT GCCCCACAGT TTTCAAAACT GTACCCTAAT 
GCCTCCCAAC ACATAACTCC GAGCTATAAC TATGCACCGA ACATGGACAA ACACTGGATC 
ATGCAGTACA CAGGTCCCAT GCTGCCTATC CACATGGAGT TCACCAACGT CCTCCAGCGC 
AAACGACTAC AGACTCTGAT GTCGGTTGAT GACTCTGTCG AGAGGCTCTA CAACATGCTT 
GTGGAGACCG GGGAACTGGG CAACACGTAC ATCATCTACA CTGCGGACCA CGGCTACCAC 
ATTGGACAGT TTGGACTGGT CAAGGGGAAA TCCATGCCCT ACGACTTTGA CATCCGCGTG 
CCTTTCTTCA TCCGTGGTCC GAGCATAGAA CCAGGGTCCA TAGTCCCACA GATTGTTCTG 
AACATTGACC TGGCCCCTAC CATTCTGGAT ATCGCCGGTC TTGACACTCC TTCTGATGTG 
GATGGCAAGT CTGTCCTGAA ACTTCTAGAC CTGGAAAAGC CGGGTAACAG GTTTCGAACA 
AACAAGAAGG CCAAAATCTG GCGTGACACA TTCCTAGTGG AAAGAGGGAA ATTTTTACGA 
AAGAAGGAGG AATCCAGCAA GAATATCCAA CAATCAAACC ACTTGCCCAA ATATGAGAGG 
GTCAAGGAAC TGTGCCAGCA GGCCAGATAC CAGACAGCCT GTGAGCAGCC TGGGCAGAAC 
TGGCAGTGCA TCGAGGACAC ATCCGGCAAG CTTCGAATCC ACAAGTGTAA GGGACCCAGC 
GACCTGCTCA CCGTCCGACA GAACGCACGC AACCTCTACT CTCGAGGATT GCAGGACAAA 
GACAAAGAGT GCCATTGTAG GGAGTCTGGT TATCGCCCGA GCAGAAGCCA GAGAAAGAAA 
GAAAGGCAGT TCCTGAGGAA CCAGGGAACA CCAAAGTATA AGCCCAGATT TGTGCACACC 
AGGCAGACTC GGTCCCTGTC GGTTGAATTC GAAGGTGAAA TATATGACAT AAATCTGGAG 
GAAGAAGAAT TGCAAGTGTT ACCGCCAAGG AGCGTTGCTA AGCGCCATGA TGAGGGCCAC 
CAGGGCTTCG GAGGCCACCA GGCTGCTGCT GGTGACTTCA GGAATGAGAT GCTGGCAGAC 
AGCAACAGTG CTGTGGGGTT ACCCACCACT GTCCGGGTGA CACACAAATG CTTTATTCTT 
CCAAATGACA CAATCCATTG TGAGAGGGAG CTGTACCAGT CGGCCAGAGC GTGGAAGGAC 
CACAAGGCCT ACATCGATAA AGAGATTGAA GTTCTACAGG ATAAAATTAA GAATTTAAGG 
GAAGTGAGGG GACACCTAAA GAAAAGGAAG CCTGAGGAGT GTAGCTGTGG TAAGCAGAGC 
TATTACAACA AAGAGAAAGG TGTCAAACGA CAGGAGAAGC TGAAGAGCCA CCTTCACCCC 
TTCAAGGAGG CTGCGGCCCA GGAGGTGGAC AGCAAACTGC AGCTGTTCAA GGAGCATCGT 
CGGAGGAAGA AGGAGAGGAA GGAGAAGAAA CGTCAGAGAA AAGGAGAAGA GTGCAGCCTG 
CCTGGCCTCA CCTGCTTCAC CCACGACAAC AACCACTGGC AGACCGCCCC GTTCTGGAAC 
TTGGGATCTT TCTGTGCATG CACGAGTTCT AACAACAATA CCTACTGGTG TTTGCGTACG 
GTCAATGAGA CGCACAATTT TCTCTTTTGT GAGTTTGCTA CTGGCTTTCT GGAGTATTTT 
GACATGAACA CAGATCCTTA CCAGCTTACA AACACGGTGC ACACGATAGA ACGGGGCATC 
TTGAATCAGC TACACATACA GCTGATGGAG CTCCGAAGCT GCCAAGGGTA TAAACAGTGC 
AACCCAAGAC CCAAGAGCCT TGACGTTGAG GACAGTTATG GGATGGATGG GAAGGTTAGT 
TGGTCCAGTG TCGCTTCAGA CACCAACTGG CAAGGCCTGG AGGAGTTATC TGGTGCAAAC 
GACATCAAAG AGGACAGATC TAACCCTAGA CTGAGGCCGG AGGCCTGGAC CAATTACCTG 
AGGGATGTCC ACAGAGCCTT TGCACTGCTG AACAGTCACC CTGATCCAAA CCAAGTAAAT 
GGGACTCCAA CTGCACCAAG CGGTGGCCTC CCAGTCACCT CTGCTGAGCT CTTGGTGCCG 
GCAGAGATGG CTTCTGCAGA GTCAGGTGAA GACCCAAGTC ATGTGGTTGG GGAAACGCCT 
CCTTTGACCT TGCCAGCCAA CCTCCAAACC CTGCATCCGA ACAGACCAAC GTTGAGTCCA 
GAGAGAAAAC TTGAATGGAA TAACGACATT CCAGAAGTGA ATCGTTTGAA TTCTGAACAC 
TGGAGAAAAA CTGAGGAGCA GCCAGGACGG GGGGAGGTGC TTCTCCCCGA AGGTGACGTC 
AGTGGCAACG GTATGACAGA GCTGTTGCCC ATCGGTCGGC ACCAACAAAA GCGTCCCCAC 
GATGCGGGGC CAGAGGACCA TGCTTTTGAA GATCAATTGC ATCCTCTCGT CCACTCTGAC 
AGAACTCCCG TTCATCGGGT GTTCGATGTG TCCCACTTGG AGCAGCCTGT TCACTCCAGC 
CACGTGGAAG GAATGTTGGC CAAGATGGAG GGGATGGCAC AAAGGAGTGG GCACCAAGTC 
TCGAAGGCAG CGCCTCCTCT CCAGTCACTT CTGCTTAGAT TACATGTTGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sulf1 ( XM_008763488.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761710.1 Rattus norvegicus sulfatase 1 (Sulf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa44313
Clone ID Related Accession (Same CDS sequence)	XM_008763486.1 , XM_008763487.2 , XM_008763488.2 , XM_017593137.1
Accession Version	XM_008763488.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	extracellular sulfatase Sulf-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAATT CCTGCTGGGC TCTGCTGCTG GCTGTGCTGG GCGCAGAACT TCTGGGAGGC 
TTCTGCTCCA CAATGCGGTC CCAGAGGTTC CGAGGACGGG TGCAGCAGGA ACGAAAAAAC 
ATCAGACCCA ATATTATCCT TGTGCTCACC GACGACCAGG ACGTGGAGTT GGGTTCCCTG 
CAAGTCATGA ACAAGACGAG AAAGATCATG GAACACGGTG GGGCCACCTT CACCAATGCC 
TTTGTGACCA CGCCCATGTG CTGCCCGTCA CGCTCCTCCA TGCTCACTGG GAAGTACGTG 
CATAACCACA ACGTCTACAC CAACAATGAG AACTGCTCTT CTCCCTCGTG GCAGGCTCTG 
CATGAGCCTC GGACCTTTGC TGTGTATCTC AACAATACTG GCTACAGAAC AGCCTTTTTT 
GGAAAATACC TCAATGAGTA TAACGGTAGC TACATCCCTC CTGGATGGCG AGAATGGCTC 
GGATTAATCA AGAATTCGCG TTTCTATAAT TACACTGTTT GTCGCAATGG CATCAAAGAG 
AAGCACGGCT TTGATTATGC AAAGGATTAC TTCACAGACT TAATCACTAA CGAGAGCATT 
AATTACTTCA AAATGTCTAA GAGAATGTAT CCCCATAGGC CTGTTATGAT GGTGATCAGC 
CACGCAGCGC CACACGGCCC TGAAGACTCT GCCCCACAGT TTTCAAAACT GTACCCTAAT 
GCCTCCCAAC ACATAACTCC GAGCTATAAC TATGCACCGA ACATGGACAA ACACTGGATC 
ATGCAGTACA CAGGTCCCAT GCTGCCTATC CACATGGAGT TCACCAACGT CCTCCAGCGC 
AAACGACTAC AGACTCTGAT GTCGGTTGAT GACTCTGTCG AGAGGCTCTA CAACATGCTT 
GTGGAGACCG GGGAACTGGG CAACACGTAC ATCATCTACA CTGCGGACCA CGGCTACCAC 
ATTGGACAGT TTGGACTGGT CAAGGGGAAA TCCATGCCCT ACGACTTTGA CATCCGCGTG 
CCTTTCTTCA TCCGTGGTCC GAGCATAGAA CCAGGGTCCA TAGTCCCACA GATTGTTCTG 
AACATTGACC TGGCCCCTAC CATTCTGGAT ATCGCCGGTC TTGACACTCC TTCTGATGTG 
GATGGCAAGT CTGTCCTGAA ACTTCTAGAC CTGGAAAAGC CGGGTAACAG GTTTCGAACA 
AACAAGAAGG CCAAAATCTG GCGTGACACA TTCCTAGTGG AAAGAGGGAA ATTTTTACGA 
AAGAAGGAGG AATCCAGCAA GAATATCCAA CAATCAAACC ACTTGCCCAA ATATGAGAGG 
GTCAAGGAAC TGTGCCAGCA GGCCAGATAC CAGACAGCCT GTGAGCAGCC TGGGCAGAAC 
TGGCAGTGCA TCGAGGACAC ATCCGGCAAG CTTCGAATCC ACAAGTGTAA GGGACCCAGC 
GACCTGCTCA CCGTCCGACA GAACGCACGC AACCTCTACT CTCGAGGATT GCAGGACAAA 
GACAAAGAGT GCCATTGTAG GGAGTCTGGT TATCGCCCGA GCAGAAGCCA GAGAAAGAAA 
GAAAGGCAGT TCCTGAGGAA CCAGGGAACA CCAAAGTATA AGCCCAGATT TGTGCACACC 
AGGCAGACTC GGTCCCTGTC GGTTGAATTC GAAGGTGAAA TATATGACAT AAATCTGGAG 
GAAGAAGAAT TGCAAGTGTT ACCGCCAAGG AGCGTTGCTA AGCGCCATGA TGAGGGCCAC 
CAGGGCTTCG GAGGCCACCA GGCTGCTGCT GGTGACTTCA GGAATGAGAT GCTGGCAGAC 
AGCAACAGTG CTGTGGGGTT ACCCACCACT GTCCGGGTGA CACACAAATG CTTTATTCTT 
CCAAATGACA CAATCCATTG TGAGAGGGAG CTGTACCAGT CGGCCAGAGC GTGGAAGGAC 
CACAAGGCCT ACATCGATAA AGAGATTGAA GTTCTACAGG ATAAAATTAA GAATTTAAGG 
GAAGTGAGGG GACACCTAAA GAAAAGGAAG CCTGAGGAGT GTAGCTGTGG TAAGCAGAGC 
TATTACAACA AAGAGAAAGG TGTCAAACGA CAGGAGAAGC TGAAGAGCCA CCTTCACCCC 
TTCAAGGAGG CTGCGGCCCA GGAGGTGGAC AGCAAACTGC AGCTGTTCAA GGAGCATCGT 
CGGAGGAAGA AGGAGAGGAA GGAGAAGAAA CGTCAGAGAA AAGGAGAAGA GTGCAGCCTG 
CCTGGCCTCA CCTGCTTCAC CCACGACAAC AACCACTGGC AGACCGCCCC GTTCTGGAAC 
TTGGGATCTT TCTGTGCATG CACGAGTTCT AACAACAATA CCTACTGGTG TTTGCGTACG 
GTCAATGAGA CGCACAATTT TCTCTTTTGT GAGTTTGCTA CTGGCTTTCT GGAGTATTTT 
GACATGAACA CAGATCCTTA CCAGCTTACA AACACGGTGC ACACGATAGA ACGGGGCATC 
TTGAATCAGC TACACATACA GCTGATGGAG CTCCGAAGCT GCCAAGGGTA TAAACAGTGC 
AACCCAAGAC CCAAGAGCCT TGACGTTGAG GACAGTTATG GGATGGATGG GAAGGTTAGT 
TGGTCCAGTG TCGCTTCAGA CACCAACTGG CAAGGCCTGG AGGAGTTATC TGGTGCAAAC 
GACATCAAAG AGGACAGATC TAACCCTAGA CTGAGGCCGG AGGCCTGGAC CAATTACCTG 
AGGGATGTCC ACAGAGCCTT TGCACTGCTG AACAGTCACC CTGATCCAAA CCAAGTAAAT 
GGGACTCCAA CTGCACCAAG CGGTGGCCTC CCAGTCACCT CTGCTGAGCT CTTGGTGCCG 
GCAGAGATGG CTTCTGCAGA GTCAGGTGAA GACCCAAGTC ATGTGGTTGG GGAAACGCCT 
CCTTTGACCT TGCCAGCCAA CCTCCAAACC CTGCATCCGA ACAGACCAAC GTTGAGTCCA 
GAGAGAAAAC TTGAATGGAA TAACGACATT CCAGAAGTGA ATCGTTTGAA TTCTGAACAC 
TGGAGAAAAA CTGAGGAGCA GCCAGGACGG GGGGAGGTGC TTCTCCCCGA AGGTGACGTC 
AGTGGCAACG GTATGACAGA GCTGTTGCCC ATCGGTCGGC ACCAACAAAA GCGTCCCCAC 
GATGCGGGGC CAGAGGACCA TGCTTTTGAA GATCAATTGC ATCCTCTCGT CCACTCTGAC 
AGAACTCCCG TTCATCGGGT GTTCGATGTG TCCCACTTGG AGCAGCCTGT TCACTCCAGC 
CACGTGGAAG GAATGTTGGC CAAGATGGAG GGGATGGCAC AAAGGAGTGG GCACCAAGTC 
TCGAAGGCAG CGCCTCCTCT CCAGTCACTT CTGCTTAGAT TACATGTTGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761710.1
CDS	591..3944
Translation	MKNSCWALLLAVLGAELLGGFCSTMRSQRFRGRVQQERKNIRPNIILVLTDDQDVELGSLQVMNKTRKIMEHGGATFTNAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSWQALHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTNVLQRKRLQTLMSVDDSVERLYNMLVETGELGNTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSIEPGSIVPQIVLNIDLAPTILDIAGLDTPSDVDGKSVLKLLDLEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARYQTACEQPGQNWQCIEDTSGKLRIHKCKGPSDLLTVRQNARNLYSRGLQDKDKECHCRESGYRPSRSQRKKERQFLRNQGTPKYKPRFVHTRQTRSLSVEFEGEIYDINLEEEELQVLPPRSVAKRHDEGHQGFGGHQAAAGDFRNEMLADSNSAVGLPTTVRVTHKCFILPNDTIHCERELYQSARAWKDHKAYIDKEIEVLQDKIKNLREVRGHLKKRKPEECSCGKQSYYNKEKGVKRQEKLKSHLHPFKEAAAQEVDSKLQLFKEHRRRKKERKEKKRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETHNFLFCEFATGFLEYFDMNTDPYQLTNTVHTIERGILNQLHIQLMELRSCQGYKQCNPRPKSLDVEDSYGMDGKVSWSSVASDTNWQGLEELSGANDIKEDRSNPRLRPEAWTNYLRDVHRAFALLNSHPDPNQVNGTPTAPSGGLPVTSAELLVPAEMASAESGEDPSHVVGETPPLTLPANLQTLHPNRPTLSPERKLEWNNDIPEVNRLNSEHWRKTEEQPGRGEVLLPEGDVSGNGMTELLPIGRHQQKRPHDAGPEDHAFEDQLHPLVHSDRTPVHRVFDVSHLEQPVHSSHVEGMLAKMEGMAQRSGHQVSKAAPPLQSLLLRLHVA

Target ORF information:

RefSeq Version	XM_008763488.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sulfatase 1 (Sulf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763488.2

ORF Insert Sequence:

ATGAAGAATT CCTGCTGGGC TCTGCTGCTG GCTGTGCTGG GCGCAGAACT TCTGGGAGGC 
TTCTGCTCCA CAATGCGGTC CCAGAGGTTC CGAGGACGGG TGCAGCAGGA ACGAAAAAAC 
ATCAGACCCA ATATTATCCT TGTGCTCACC GACGACCAGG ACGTGGAGTT GGGTTCCCTG 
CAAGTCATGA ACAAGACGAG AAAGATCATG GAACACGGTG GGGCCACCTT CACCAATGCC 
TTTGTGACCA CGCCCATGTG CTGCCCGTCA CGCTCCTCCA TGCTCACTGG GAAGTACGTG 
CATAACCACA ACGTCTACAC CAACAATGAG AACTGCTCTT CTCCCTCGTG GCAGGCTCTG 
CATGAGCCTC GGACCTTTGC TGTGTATCTC AACAATACTG GCTACAGAAC AGCCTTTTTT 
GGAAAATACC TCAATGAGTA TAACGGTAGC TACATCCCTC CTGGATGGCG AGAATGGCTC 
GGATTAATCA AGAATTCGCG TTTCTATAAT TACACTGTTT GTCGCAATGG CATCAAAGAG 
AAGCACGGCT TTGATTATGC AAAGGATTAC TTCACAGACT TAATCACTAA CGAGAGCATT 
AATTACTTCA AAATGTCTAA GAGAATGTAT CCCCATAGGC CTGTTATGAT GGTGATCAGC 
CACGCAGCGC CACACGGCCC TGAAGACTCT GCCCCACAGT TTTCAAAACT GTACCCTAAT 
GCCTCCCAAC ACATAACTCC GAGCTATAAC TATGCACCGA ACATGGACAA ACACTGGATC 
ATGCAGTACA CAGGTCCCAT GCTGCCTATC CACATGGAGT TCACCAACGT CCTCCAGCGC 
AAACGACTAC AGACTCTGAT GTCGGTTGAT GACTCTGTCG AGAGGCTCTA CAACATGCTT 
GTGGAGACCG GGGAACTGGG CAACACGTAC ATCATCTACA CTGCGGACCA CGGCTACCAC 
ATTGGACAGT TTGGACTGGT CAAGGGGAAA TCCATGCCCT ACGACTTTGA CATCCGCGTG 
CCTTTCTTCA TCCGTGGTCC GAGCATAGAA CCAGGGTCCA TAGTCCCACA GATTGTTCTG 
AACATTGACC TGGCCCCTAC CATTCTGGAT ATCGCCGGTC TTGACACTCC TTCTGATGTG 
GATGGCAAGT CTGTCCTGAA ACTTCTAGAC CTGGAAAAGC CGGGTAACAG GTTTCGAACA 
AACAAGAAGG CCAAAATCTG GCGTGACACA TTCCTAGTGG AAAGAGGGAA ATTTTTACGA 
AAGAAGGAGG AATCCAGCAA GAATATCCAA CAATCAAACC ACTTGCCCAA ATATGAGAGG 
GTCAAGGAAC TGTGCCAGCA GGCCAGATAC CAGACAGCCT GTGAGCAGCC TGGGCAGAAC 
TGGCAGTGCA TCGAGGACAC ATCCGGCAAG CTTCGAATCC ACAAGTGTAA GGGACCCAGC 
GACCTGCTCA CCGTCCGACA GAACGCACGC AACCTCTACT CTCGAGGATT GCAGGACAAA 
GACAAAGAGT GCCATTGTAG GGAGTCTGGT TATCGCCCGA GCAGAAGCCA GAGAAAGAAA 
GAAAGGCAGT TCCTGAGGAA CCAGGGAACA CCAAAGTATA AGCCCAGATT TGTGCACACC 
AGGCAGACTC GGTCCCTGTC GGTTGAATTC GAAGGTGAAA TATATGACAT AAATCTGGAG 
GAAGAAGAAT TGCAAGTGTT ACCGCCAAGG AGCGTTGCTA AGCGCCATGA TGAGGGCCAC 
CAGGGCTTCG GAGGCCACCA GGCTGCTGCT GGTGACTTCA GGAATGAGAT GCTGGCAGAC 
AGCAACAGTG CTGTGGGGTT ACCCACCACT GTCCGGGTGA CACACAAATG CTTTATTCTT 
CCAAATGACA CAATCCATTG TGAGAGGGAG CTGTACCAGT CGGCCAGAGC GTGGAAGGAC 
CACAAGGCCT ACATCGATAA AGAGATTGAA GTTCTACAGG ATAAAATTAA GAATTTAAGG 
GAAGTGAGGG GACACCTAAA GAAAAGGAAG CCTGAGGAGT GTAGCTGTGG TAAGCAGAGC 
TATTACAACA AAGAGAAAGG TGTCAAACGA CAGGAGAAGC TGAAGAGCCA CCTTCACCCC 
TTCAAGGAGG CTGCGGCCCA GGAGGTGGAC AGCAAACTGC AGCTGTTCAA GGAGCATCGT 
CGGAGGAAGA AGGAGAGGAA GGAGAAGAAA CGTCAGAGAA AAGGAGAAGA GTGCAGCCTG 
CCTGGCCTCA CCTGCTTCAC CCACGACAAC AACCACTGGC AGACCGCCCC GTTCTGGAAC 
TTGGGATCTT TCTGTGCATG CACGAGTTCT AACAACAATA CCTACTGGTG TTTGCGTACG 
GTCAATGAGA CGCACAATTT TCTCTTTTGT GAGTTTGCTA CTGGCTTTCT GGAGTATTTT 
GACATGAACA CAGATCCTTA CCAGCTTACA AACACGGTGC ACACGATAGA ACGGGGCATC 
TTGAATCAGC TACACATACA GCTGATGGAG CTCCGAAGCT GCCAAGGGTA TAAACAGTGC 
AACCCAAGAC CCAAGAGCCT TGACGTTGAG GACAGTTATG GGATGGATGG GAAGGTTAGT 
TGGTCCAGTG TCGCTTCAGA CACCAACTGG CAAGGCCTGG AGGAGTTATC TGGTGCAAAC 
GACATCAAAG AGGACAGATC TAACCCTAGA CTGAGGCCGG AGGCCTGGAC CAATTACCTG 
AGGGATGTCC ACAGAGCCTT TGCACTGCTG AACAGTCACC CTGATCCAAA CCAAGTAAAT 
GGGACTCCAA CTGCACCAAG CGGTGGCCTC CCAGTCACCT CTGCTGAGCT CTTGGTGCCG 
GCAGAGATGG CTTCTGCAGA GTCAGGTGAA GACCCAAGTC ATGTGGTTGG GGAAACGCCT 
CCTTTGACCT TGCCAGCCAA CCTCCAAACC CTGCATCCGA ACAGACCAAC GTTGAGTCCA 
GAGAGAAAAC TTGAATGGAA TAACGACATT CCAGAAGTGA ATCGTTTGAA TTCTGAACAC 
TGGAGAAAAA CTGAGGAGCA GCCAGGACGG GGGGAGGTGC TTCTCCCCGA AGGTGACGTC 
AGTGGCAACG GTATGACAGA GCTGTTGCCC ATCGGTCGGC ACCAACAAAA GCGTCCCCAC 
GATGCGGGGC CAGAGGACCA TGCTTTTGAA GATCAATTGC ATCCTCTCGT CCACTCTGAC 
AGAACTCCCG TTCATCGGGT GTTCGATGTG TCCCACTTGG AGCAGCCTGT TCACTCCAGC 
CACGTGGAAG GAATGTTGGC CAAGATGGAG GGGATGGCAC AAAGGAGTGG GCACCAAGTC 
TCGAAGGCAG CGCCTCCTCT CCAGTCACTT CTGCTTAGAT TACATGTTGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sulf1 ( XM_017593137.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448626.1 Rattus norvegicus sulfatase 1 (Sulf1), transcript variant X4, mRNA.

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CloneID	ORa44313
Clone ID Related Accession (Same CDS sequence)	XM_008763486.1 , XM_008763487.2 , XM_008763488.2 , XM_017593137.1
Accession Version	XM_017593137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	extracellular sulfatase Sulf-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAATT CCTGCTGGGC TCTGCTGCTG GCTGTGCTGG GCGCAGAACT TCTGGGAGGC 
TTCTGCTCCA CAATGCGGTC CCAGAGGTTC CGAGGACGGG TGCAGCAGGA ACGAAAAAAC 
ATCAGACCCA ATATTATCCT TGTGCTCACC GACGACCAGG ACGTGGAGTT GGGTTCCCTG 
CAAGTCATGA ACAAGACGAG AAAGATCATG GAACACGGTG GGGCCACCTT CACCAATGCC 
TTTGTGACCA CGCCCATGTG CTGCCCGTCA CGCTCCTCCA TGCTCACTGG GAAGTACGTG 
CATAACCACA ACGTCTACAC CAACAATGAG AACTGCTCTT CTCCCTCGTG GCAGGCTCTG 
CATGAGCCTC GGACCTTTGC TGTGTATCTC AACAATACTG GCTACAGAAC AGCCTTTTTT 
GGAAAATACC TCAATGAGTA TAACGGTAGC TACATCCCTC CTGGATGGCG AGAATGGCTC 
GGATTAATCA AGAATTCGCG TTTCTATAAT TACACTGTTT GTCGCAATGG CATCAAAGAG 
AAGCACGGCT TTGATTATGC AAAGGATTAC TTCACAGACT TAATCACTAA CGAGAGCATT 
AATTACTTCA AAATGTCTAA GAGAATGTAT CCCCATAGGC CTGTTATGAT GGTGATCAGC 
CACGCAGCGC CACACGGCCC TGAAGACTCT GCCCCACAGT TTTCAAAACT GTACCCTAAT 
GCCTCCCAAC ACATAACTCC GAGCTATAAC TATGCACCGA ACATGGACAA ACACTGGATC 
ATGCAGTACA CAGGTCCCAT GCTGCCTATC CACATGGAGT TCACCAACGT CCTCCAGCGC 
AAACGACTAC AGACTCTGAT GTCGGTTGAT GACTCTGTCG AGAGGCTCTA CAACATGCTT 
GTGGAGACCG GGGAACTGGG CAACACGTAC ATCATCTACA CTGCGGACCA CGGCTACCAC 
ATTGGACAGT TTGGACTGGT CAAGGGGAAA TCCATGCCCT ACGACTTTGA CATCCGCGTG 
CCTTTCTTCA TCCGTGGTCC GAGCATAGAA CCAGGGTCCA TAGTCCCACA GATTGTTCTG 
AACATTGACC TGGCCCCTAC CATTCTGGAT ATCGCCGGTC TTGACACTCC TTCTGATGTG 
GATGGCAAGT CTGTCCTGAA ACTTCTAGAC CTGGAAAAGC CGGGTAACAG GTTTCGAACA 
AACAAGAAGG CCAAAATCTG GCGTGACACA TTCCTAGTGG AAAGAGGGAA ATTTTTACGA 
AAGAAGGAGG AATCCAGCAA GAATATCCAA CAATCAAACC ACTTGCCCAA ATATGAGAGG 
GTCAAGGAAC TGTGCCAGCA GGCCAGATAC CAGACAGCCT GTGAGCAGCC TGGGCAGAAC 
TGGCAGTGCA TCGAGGACAC ATCCGGCAAG CTTCGAATCC ACAAGTGTAA GGGACCCAGC 
GACCTGCTCA CCGTCCGACA GAACGCACGC AACCTCTACT CTCGAGGATT GCAGGACAAA 
GACAAAGAGT GCCATTGTAG GGAGTCTGGT TATCGCCCGA GCAGAAGCCA GAGAAAGAAA 
GAAAGGCAGT TCCTGAGGAA CCAGGGAACA CCAAAGTATA AGCCCAGATT TGTGCACACC 
AGGCAGACTC GGTCCCTGTC GGTTGAATTC GAAGGTGAAA TATATGACAT AAATCTGGAG 
GAAGAAGAAT TGCAAGTGTT ACCGCCAAGG AGCGTTGCTA AGCGCCATGA TGAGGGCCAC 
CAGGGCTTCG GAGGCCACCA GGCTGCTGCT GGTGACTTCA GGAATGAGAT GCTGGCAGAC 
AGCAACAGTG CTGTGGGGTT ACCCACCACT GTCCGGGTGA CACACAAATG CTTTATTCTT 
CCAAATGACA CAATCCATTG TGAGAGGGAG CTGTACCAGT CGGCCAGAGC GTGGAAGGAC 
CACAAGGCCT ACATCGATAA AGAGATTGAA GTTCTACAGG ATAAAATTAA GAATTTAAGG 
GAAGTGAGGG GACACCTAAA GAAAAGGAAG CCTGAGGAGT GTAGCTGTGG TAAGCAGAGC 
TATTACAACA AAGAGAAAGG TGTCAAACGA CAGGAGAAGC TGAAGAGCCA CCTTCACCCC 
TTCAAGGAGG CTGCGGCCCA GGAGGTGGAC AGCAAACTGC AGCTGTTCAA GGAGCATCGT 
CGGAGGAAGA AGGAGAGGAA GGAGAAGAAA CGTCAGAGAA AAGGAGAAGA GTGCAGCCTG 
CCTGGCCTCA CCTGCTTCAC CCACGACAAC AACCACTGGC AGACCGCCCC GTTCTGGAAC 
TTGGGATCTT TCTGTGCATG CACGAGTTCT AACAACAATA CCTACTGGTG TTTGCGTACG 
GTCAATGAGA CGCACAATTT TCTCTTTTGT GAGTTTGCTA CTGGCTTTCT GGAGTATTTT 
GACATGAACA CAGATCCTTA CCAGCTTACA AACACGGTGC ACACGATAGA ACGGGGCATC 
TTGAATCAGC TACACATACA GCTGATGGAG CTCCGAAGCT GCCAAGGGTA TAAACAGTGC 
AACCCAAGAC CCAAGAGCCT TGACGTTGAG GACAGTTATG GGATGGATGG GAAGGTTAGT 
TGGTCCAGTG TCGCTTCAGA CACCAACTGG CAAGGCCTGG AGGAGTTATC TGGTGCAAAC 
GACATCAAAG AGGACAGATC TAACCCTAGA CTGAGGCCGG AGGCCTGGAC CAATTACCTG 
AGGGATGTCC ACAGAGCCTT TGCACTGCTG AACAGTCACC CTGATCCAAA CCAAGTAAAT 
GGGACTCCAA CTGCACCAAG CGGTGGCCTC CCAGTCACCT CTGCTGAGCT CTTGGTGCCG 
GCAGAGATGG CTTCTGCAGA GTCAGGTGAA GACCCAAGTC ATGTGGTTGG GGAAACGCCT 
CCTTTGACCT TGCCAGCCAA CCTCCAAACC CTGCATCCGA ACAGACCAAC GTTGAGTCCA 
GAGAGAAAAC TTGAATGGAA TAACGACATT CCAGAAGTGA ATCGTTTGAA TTCTGAACAC 
TGGAGAAAAA CTGAGGAGCA GCCAGGACGG GGGGAGGTGC TTCTCCCCGA AGGTGACGTC 
AGTGGCAACG GTATGACAGA GCTGTTGCCC ATCGGTCGGC ACCAACAAAA GCGTCCCCAC 
GATGCGGGGC CAGAGGACCA TGCTTTTGAA GATCAATTGC ATCCTCTCGT CCACTCTGAC 
AGAACTCCCG TTCATCGGGT GTTCGATGTG TCCCACTTGG AGCAGCCTGT TCACTCCAGC 
CACGTGGAAG GAATGTTGGC CAAGATGGAG GGGATGGCAC AAAGGAGTGG GCACCAAGTC 
TCGAAGGCAG CGCCTCCTCT CCAGTCACTT CTGCTTAGAT TACATGTTGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448626.1
CDS	2211..5564
Translation	MKNSCWALLLAVLGAELLGGFCSTMRSQRFRGRVQQERKNIRPNIILVLTDDQDVELGSLQVMNKTRKIMEHGGATFTNAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSWQALHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTNVLQRKRLQTLMSVDDSVERLYNMLVETGELGNTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSIEPGSIVPQIVLNIDLAPTILDIAGLDTPSDVDGKSVLKLLDLEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARYQTACEQPGQNWQCIEDTSGKLRIHKCKGPSDLLTVRQNARNLYSRGLQDKDKECHCRESGYRPSRSQRKKERQFLRNQGTPKYKPRFVHTRQTRSLSVEFEGEIYDINLEEEELQVLPPRSVAKRHDEGHQGFGGHQAAAGDFRNEMLADSNSAVGLPTTVRVTHKCFILPNDTIHCERELYQSARAWKDHKAYIDKEIEVLQDKIKNLREVRGHLKKRKPEECSCGKQSYYNKEKGVKRQEKLKSHLHPFKEAAAQEVDSKLQLFKEHRRRKKERKEKKRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETHNFLFCEFATGFLEYFDMNTDPYQLTNTVHTIERGILNQLHIQLMELRSCQGYKQCNPRPKSLDVEDSYGMDGKVSWSSVASDTNWQGLEELSGANDIKEDRSNPRLRPEAWTNYLRDVHRAFALLNSHPDPNQVNGTPTAPSGGLPVTSAELLVPAEMASAESGEDPSHVVGETPPLTLPANLQTLHPNRPTLSPERKLEWNNDIPEVNRLNSEHWRKTEEQPGRGEVLLPEGDVSGNGMTELLPIGRHQQKRPHDAGPEDHAFEDQLHPLVHSDRTPVHRVFDVSHLEQPVHSSHVEGMLAKMEGMAQRSGHQVSKAAPPLQSLLLRLHVA

Target ORF information:

RefSeq Version	XM_017593137.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sulfatase 1 (Sulf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593137.1

ORF Insert Sequence:

ATGAAGAATT CCTGCTGGGC TCTGCTGCTG GCTGTGCTGG GCGCAGAACT TCTGGGAGGC 
TTCTGCTCCA CAATGCGGTC CCAGAGGTTC CGAGGACGGG TGCAGCAGGA ACGAAAAAAC 
ATCAGACCCA ATATTATCCT TGTGCTCACC GACGACCAGG ACGTGGAGTT GGGTTCCCTG 
CAAGTCATGA ACAAGACGAG AAAGATCATG GAACACGGTG GGGCCACCTT CACCAATGCC 
TTTGTGACCA CGCCCATGTG CTGCCCGTCA CGCTCCTCCA TGCTCACTGG GAAGTACGTG 
CATAACCACA ACGTCTACAC CAACAATGAG AACTGCTCTT CTCCCTCGTG GCAGGCTCTG 
CATGAGCCTC GGACCTTTGC TGTGTATCTC AACAATACTG GCTACAGAAC AGCCTTTTTT 
GGAAAATACC TCAATGAGTA TAACGGTAGC TACATCCCTC CTGGATGGCG AGAATGGCTC 
GGATTAATCA AGAATTCGCG TTTCTATAAT TACACTGTTT GTCGCAATGG CATCAAAGAG 
AAGCACGGCT TTGATTATGC AAAGGATTAC TTCACAGACT TAATCACTAA CGAGAGCATT 
AATTACTTCA AAATGTCTAA GAGAATGTAT CCCCATAGGC CTGTTATGAT GGTGATCAGC 
CACGCAGCGC CACACGGCCC TGAAGACTCT GCCCCACAGT TTTCAAAACT GTACCCTAAT 
GCCTCCCAAC ACATAACTCC GAGCTATAAC TATGCACCGA ACATGGACAA ACACTGGATC 
ATGCAGTACA CAGGTCCCAT GCTGCCTATC CACATGGAGT TCACCAACGT CCTCCAGCGC 
AAACGACTAC AGACTCTGAT GTCGGTTGAT GACTCTGTCG AGAGGCTCTA CAACATGCTT 
GTGGAGACCG GGGAACTGGG CAACACGTAC ATCATCTACA CTGCGGACCA CGGCTACCAC 
ATTGGACAGT TTGGACTGGT CAAGGGGAAA TCCATGCCCT ACGACTTTGA CATCCGCGTG 
CCTTTCTTCA TCCGTGGTCC GAGCATAGAA CCAGGGTCCA TAGTCCCACA GATTGTTCTG 
AACATTGACC TGGCCCCTAC CATTCTGGAT ATCGCCGGTC TTGACACTCC TTCTGATGTG 
GATGGCAAGT CTGTCCTGAA ACTTCTAGAC CTGGAAAAGC CGGGTAACAG GTTTCGAACA 
AACAAGAAGG CCAAAATCTG GCGTGACACA TTCCTAGTGG AAAGAGGGAA ATTTTTACGA 
AAGAAGGAGG AATCCAGCAA GAATATCCAA CAATCAAACC ACTTGCCCAA ATATGAGAGG 
GTCAAGGAAC TGTGCCAGCA GGCCAGATAC CAGACAGCCT GTGAGCAGCC TGGGCAGAAC 
TGGCAGTGCA TCGAGGACAC ATCCGGCAAG CTTCGAATCC ACAAGTGTAA GGGACCCAGC 
GACCTGCTCA CCGTCCGACA GAACGCACGC AACCTCTACT CTCGAGGATT GCAGGACAAA 
GACAAAGAGT GCCATTGTAG GGAGTCTGGT TATCGCCCGA GCAGAAGCCA GAGAAAGAAA 
GAAAGGCAGT TCCTGAGGAA CCAGGGAACA CCAAAGTATA AGCCCAGATT TGTGCACACC 
AGGCAGACTC GGTCCCTGTC GGTTGAATTC GAAGGTGAAA TATATGACAT AAATCTGGAG 
GAAGAAGAAT TGCAAGTGTT ACCGCCAAGG AGCGTTGCTA AGCGCCATGA TGAGGGCCAC 
CAGGGCTTCG GAGGCCACCA GGCTGCTGCT GGTGACTTCA GGAATGAGAT GCTGGCAGAC 
AGCAACAGTG CTGTGGGGTT ACCCACCACT GTCCGGGTGA CACACAAATG CTTTATTCTT 
CCAAATGACA CAATCCATTG TGAGAGGGAG CTGTACCAGT CGGCCAGAGC GTGGAAGGAC 
CACAAGGCCT ACATCGATAA AGAGATTGAA GTTCTACAGG ATAAAATTAA GAATTTAAGG 
GAAGTGAGGG GACACCTAAA GAAAAGGAAG CCTGAGGAGT GTAGCTGTGG TAAGCAGAGC 
TATTACAACA AAGAGAAAGG TGTCAAACGA CAGGAGAAGC TGAAGAGCCA CCTTCACCCC 
TTCAAGGAGG CTGCGGCCCA GGAGGTGGAC AGCAAACTGC AGCTGTTCAA GGAGCATCGT 
CGGAGGAAGA AGGAGAGGAA GGAGAAGAAA CGTCAGAGAA AAGGAGAAGA GTGCAGCCTG 
CCTGGCCTCA CCTGCTTCAC CCACGACAAC AACCACTGGC AGACCGCCCC GTTCTGGAAC 
TTGGGATCTT TCTGTGCATG CACGAGTTCT AACAACAATA CCTACTGGTG TTTGCGTACG 
GTCAATGAGA CGCACAATTT TCTCTTTTGT GAGTTTGCTA CTGGCTTTCT GGAGTATTTT 
GACATGAACA CAGATCCTTA CCAGCTTACA AACACGGTGC ACACGATAGA ACGGGGCATC 
TTGAATCAGC TACACATACA GCTGATGGAG CTCCGAAGCT GCCAAGGGTA TAAACAGTGC 
AACCCAAGAC CCAAGAGCCT TGACGTTGAG GACAGTTATG GGATGGATGG GAAGGTTAGT 
TGGTCCAGTG TCGCTTCAGA CACCAACTGG CAAGGCCTGG AGGAGTTATC TGGTGCAAAC 
GACATCAAAG AGGACAGATC TAACCCTAGA CTGAGGCCGG AGGCCTGGAC CAATTACCTG 
AGGGATGTCC ACAGAGCCTT TGCACTGCTG AACAGTCACC CTGATCCAAA CCAAGTAAAT 
GGGACTCCAA CTGCACCAAG CGGTGGCCTC CCAGTCACCT CTGCTGAGCT CTTGGTGCCG 
GCAGAGATGG CTTCTGCAGA GTCAGGTGAA GACCCAAGTC ATGTGGTTGG GGAAACGCCT 
CCTTTGACCT TGCCAGCCAA CCTCCAAACC CTGCATCCGA ACAGACCAAC GTTGAGTCCA 
GAGAGAAAAC TTGAATGGAA TAACGACATT CCAGAAGTGA ATCGTTTGAA TTCTGAACAC 
TGGAGAAAAA CTGAGGAGCA GCCAGGACGG GGGGAGGTGC TTCTCCCCGA AGGTGACGTC 
AGTGGCAACG GTATGACAGA GCTGTTGCCC ATCGGTCGGC ACCAACAAAA GCGTCCCCAC 
GATGCGGGGC CAGAGGACCA TGCTTTTGAA GATCAATTGC ATCCTCTCGT CCACTCTGAC 
AGAACTCCCG TTCATCGGGT GTTCGATGTG TCCCACTTGG AGCAGCCTGT TCACTCCAGC 
CACGTGGAAG GAATGTTGGC CAAGATGGAG GGGATGGCAC AAAGGAGTGG GCACCAAGTC 
TCGAAGGCAG CGCCTCCTCT CCAGTCACTT CTGCTTAGAT TACATGTTGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing.
Histochemistry and cell biology145(1)67-79(2016 Jan)
Zaman G,Staines KA,Farquharson C,Newton PT,Dudhia J,Chenu C,Pitsillides AA,Dhoot GK

Increased sulfatase 1 gene expression in degenerative intervertebral disc cells.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society33(3)312-7(2015 Mar)
Tsai TT,Ho NY,Fang HC,Lai PL,Niu CC,Chen LH,Chen WJ,Pang JH

Sulf1 and Sulf2 expression in the nervous system and its role in limiting neurite outgrowth in vitro.
Experimental neurology263150-60(2015 Jan)
Joy MT,Vrbova G,Dhoot GK,Anderson PN

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Oncology reports28(2)429-38(2012 Aug)
B?hm D,Keller K,Pieter J,Boehm N,Wolters D,Siggelkow W,Lebrecht A,Schmidt M,K?lbl H,Pfeiffer N,Grus FH

WT1-dependent sulfatase expression maintains the normal glomerular filtration barrier.
Journal of the American Society of Nephrology : JASN22(7)1286-96(2011 Jul)
Schumacher VA,Schl?tzer-Schrehardt U,Karumanchi SA,Shi X,Zaia J,Jeruschke S,Zhang D,Pavenst?dt H,Pavenstaedt H,Drenckhan A,Amann K,Ng C,Hartwig S,Ng KH,Ho J,Kreidberg JA,Taglienti M,Royer-Pokora B,Ai X

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Sulf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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