Nlrp6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nlrp6
Entrez Gene ID	171390
Full Name	NLR family, pyrin domain containing 6
Synonyms	Avr,Nalp6,Navr,Navr/Avr,Non-AVR
General protein information	Preferred Names NLR family, pyrin domain containing 6 Names NACHT, LRR and PYD domains-containing protein 6 NACHT, LRR and PYD domains-containing protein 6-like NACHT, leucine rich repeat and PYD containing 6 angiotensin II/vasopressin receptor dual angiotensin II/vasopressin receptor non-angiotensin-vasopressin receptor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q41
Summary	The protein encoded by this gene binds arginine-vasopressin and may be involved in the arginine-vasopressin-mediated regulation of renal salt-water balance. The encoded protein also mediates inflammatory responses in the colon to allow recovery from intestinal epithelial damage and protects against tumorigenesis and the development of colitis. Finally, this protein can increase activation of NF-kappa-B, activation of CASP1 through interaction with ASC, and cAMP accumulation. [provided by RefSeq, Feb 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_134375.3	NP_599202.2	NACHT, LRR and PYD domains-containing protein 6

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NLRP6	XP_540513.3
Bos taurus (cattle)	NLRP6	XP_002683779.2
Rattus norvegicus (Norway rat)	LOC100912017	XP_006230648.1
	NLRP6	NP_001107586.1
Mus musculus (house mouse)	Nlrp6	NP_598707.2
Rattus norvegicus (Norway rat)	Nlrp6	NP_599202.2
Pan troglodytes (chimpanzee)	NLRP6	XP_521701.3
Homo sapiens (human)	NLRP6	NP_001263629.1

Gene Ontology
Bibliography

Related articles in PubMed

NLRP6 inflammasome orchestrates the colonic host-microbial interface by regulating goblet cell mucus secretion.
Wlodarska M, Thaiss CA, Nowarski R, Henao-Mejia J, Zhang JP, Brown EM, Frankel G, Levy M, Katz MN, Philbrick WM, Elinav E, Finlay BB, Flavell RA
Cell156(5)1045-59(2014 Feb)

Microbiota-induced activation of epithelial IL-6 signaling links inflammasome-driven inflammation with transmissible cancer.
Hu B, Elinav E, Huber S, Strowig T, Hao L, Hafemann A, Jin C, Wunderlich C, Wunderlich T, Eisenbarth SC, Flavell RA
Proceedings of the National Academy of Sciences of the United States of America110(24)9862-7(2013 Jun)

IL-22BP is regulated by the inflammasome and modulates tumorigenesis in the intestine.
Huber S, Gagliani N, Zenewicz LA, Huber FJ, Bosurgi L, Hu B, Hedl M, Zhang W, O'Connor W Jr, Murphy AJ, Valenzuela DM, Yancopoulos GD, Booth CJ, Cho JH, Ouyang W, Abraham C, Flavell RA
Nature491(7423)259-63(2012 Nov)

NLRP6 negatively regulates innate immunity and host defence against bacterial pathogens.
Anand PK, Malireddi RK, Lukens JR, Vogel P, Bertin J, Lamkanfi M, Kanneganti TD
Nature488(7411)389-93(2012 Aug)

Nod-like receptor pyrin domain-containing protein 6 (NLRP6) controls epithelial self-renewal and colorectal carcinogenesis upon injury.
Normand S, Delanoye-Crespin A, Bressenot A, Huot L, Grandjean T, Peyrin-Biroulet L, Lemoine Y, Hot D, Chamaillard M
Proceedings of the National Academy of Sciences of the United States of America108(23)9601-6(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Transcripts of core components of NOD-like receptor Nlrp6 inflammasome (Nlrp6, Pycard, Caspase-1) and one of its substrates, IL-18, were increased at E20 compared with E16 in fetal intestine and not lung.
Title: Developmental control of the Nlrp6 inflammasome and a substrate, IL-18, in mammalian intestine.

AVR is distinct from Nalp6/PYPAF5 based on different mRNA and protein sizes, subcellular localization, and tissue-specific expression patterns.
Title: Overlapping genes in Nalp6/PYPAF5 locus encode two V2-type vasopressin isoreceptors: angiotensin-vasopressin receptor (AVR) and non-AVR.

The dual AngII/AVP receptor gene N119S/C163R variant exhibits sodium-induced dysfunction and cosegregates with salt-sensitive hypertension in the Dahl salt-sensitive hypertensive rat model.
Title: The dual AngII/AVP receptor gene N119S/C163R variant exhibits sodium-induced dysfunction and cosegregates with salt-sensitive hypertension in the Dahl salt-sensitive hypertensive rat model.

The following Nlrp6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nlrp6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42773	NM_134375.3 Latest version!	Rattus norvegicus NLR family, pyrin domain containing 6 (Nlrp6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nlrp6 ( NM_134375.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_599202.2 Rattus norvegicus NLR family, pyrin domain containing 6 (Nlrp6), mRNA.

Price & Availability↑

CloneID	ORa42773
Clone ID Related Accession (Same CDS sequence)	NM_134375.3
Accession Version	NM_134375.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	NACHT, LRR and PYD domains-containing protein 6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ631800.1. On or before Oct 23, 2008 this sequence version replaced XM_001061777.1, XM_577848.2, NM_134375.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ631800.1 [ECO:0000332] ##Evidence-Data-END##

ATGGATGCCG CTGGAGCCTC CTGCTCCAGC TCGGAGGCGG TGGCAAGGGA ACTGCTTCTG 
GCCGCACTGC AGGATCTGAG CCAGGAGCAG CTGAAGCGTT TTCGCCACAA GCTCCGGGAT 
GCGCCCCTGG ATGGCCGCAG CATCCCGTGG GGGCGACTGG AGCACTCAGA CGCTGTGGAC 
CTCACAGACA AGCTGATTGA GTTCTATGCG CCAGAGCCAG CCGTAGATGT GACCCGAAAG 
ATCCTGAAGA AGGCGGATAT TCGAGATGTG TCGTTGCGAC TCAAGGAGCA GCAGCTGCAG 
AGGCTCGGCT CCAGCTCGGC GCTTCTCACC GTGTCGGAGT ACAAGAAGAA GTACCGCGAG 
CACGTGCTAC GACAGCATGC TAAGGTGAAG GAGAGGAATG CCCGCTCCGT GAAGATCAAC 
AAGCGCTTCA CCAAGCTGCT CATTGCGCCG GGGAGCGGCG CCGGGGAGGA CGAGCTGCTG 
GGAACGTCGG GGGAGCCCGA GCCCGAGCGC GCAAGGCGCT CGGACACACA CACTTTCAAT 
CGGCTCTTCC GAGGAAATGA TGACGAGGGC CCGCGGCCGC TGACCGTGGT GCTGCAGGGC 
CCGGCAGGCA TCGGCAAAAC CATGGCCGCC AAGAAAATCC TCTACGACTG GGCAGGAGGC 
AAGCTATACC ACAGCCAAGT GGACTTTGCC TTCTTCATGC CGTGCGGCGA GCTGCTGGAG 
CGGCCAGGTA CGCGCAGCTT GGCGGACCTG ATACTGGAAC AGTGCCCGGA CCGCACCGCG 
CCGGTGCGGC GGATACTAGC GCAACCGCAT CGTCTACTGT TCATCCTGGA CGGTGCAGAC 
GAATTGCCTA CTTTAGCAGC CCCAGAAGCC ACTCCCTGCA GAGACCCTTT CGAGGCTACG 
AGTGGCTTGA GAGTGCTGAG CGGGCTGCTG AGCCAGGAGC TGTTACCAGC GGCTCGCTTG 
CTGGTGACTT CACGCAACGC CACCCTCGGG AGGCTGCAGG GCAGACTTTG CTCACCACAG 
TGCGCAGAAG TGCGCGGCTT CTCAGACAAA GACAAGAAAA AGTATTTCTT CAAGTTTTTC 
CGGGACGAGA GGAAGGCAGA GCGCGCTTAC CGCTTCGTGA AAGAGAATGA GACGCTGTAC 
GCACTGTGCT TTGTGCCGTT TGTGTGCTGG ATCGTGTGCA CCGTGCTGCT GCAGCAAATG 
GAGCTGGGCC GGGATCTGTC TCGTACCTCC AAGACCACTA CATCTGTGTA CCTGCTCTTC 
ATCACCAGCA TGCTGAAGTC TGCAGGTACC AATGGACCCC GGGTTCAGGG AGAGCTGCGA 
ATGCTGTGCC GCCTGGCCCG GGAGGGCATC CTGAAGCATC AAGCACAGTT CTCAGAAAAG 
GACCTGGAGA GATTGAAGCT TCAGGGTTCC CAAGTTCAGA CAATGTTTCT CAGCAAGAAG 
GAGCTGCCAG GAGTGCTAGA AACTGTGGTC ACCTACCAGT TCATTGACCA GAGCTTCCAG 
GAGTTCTTGG CTGCATTGTC ATACCTACTA GACGCTGAGG GAGCCCCAGG GAACTCCGCA 
GGAAGTGTGC AGATGCTCCT GAACTCTGAC GCGGGGCTGC GTGGTCATCT GGCACTCACC 
ACTAGATTCC TCTTTGGACT GCTAAGTACA GAGAGGATTC GTGACATTGG AAACCATTTT 
GGCTGTGTGG TGCCAGGGCG TGTGAAACAG GACACCTTGC GGTGGGTACA AGGACAAAGC 
CAACCCAAGG TGGCGACAGT AGGAGCAGAA AAGAAGGATG AGCTGAAGGA CGAGGAAGCA 
GAGGAGGAGG AGGAGGAGGA AGAAGAGGAG GAGGAACTCA ACTTTGGACT GGAGCTGTTG 
TACTGCCTGT ATGAGACACA AGAGGATGAT TTTGTTCGCC AGGCTCTCAG CAGCCTTCCA 
GAGATGGTAC TGGAGCGAGT TAGGCTGACC CGCATGGACC TTGAGGTTCT GAGCTACTGC 
GTGCAGTGCT GCCCGGACGG CCAGGCTCTG AGACTGGTGA GCTGTGGACT GGTGGCGGCA 
AAGGAGAAGA AGAAGAAGAA GAAGAGCTTC ATGAACCGGC TGAAGGGTTC TCAAAGCACC 
GGGAAACAAC CCCCAGCCTC CTTGCTGCGT CCACTCTGTG AGGCAATGAT TACCCAGCAA 
TGTGGTCTGA GTATTCTGAC CTTGTCACAC TGCAAACTCC CTGATGCAGT TTGTCGAGAC 
CTTTCTGAGG CTCTGAAGGT AGCTCCTTCC CTAAGGGAGC TGGGCCTCCT CCAGAACCGA 
CTCACTGAGG CAGGCCTGCG TTTACTAAGC CAAGGCCTGG CTTGGCCCAA ATGCAAGGTG 
CAGACACTCA GGATACAGAT GCCTGGCCTC CAAGAGGTGA TCCACTACCT GGTCATTGTG 
CTCCAGCAGA GCCCAGTCCT AACCACTCTG GACCTCAGTG GCTGTCAGCT GCCTGGCACT 
GTGGTGGAAC CTCTGTGTTC AGCCCTGAAG CACCCTAAAT GTGGCCTAAA GACCCTCAGT 
CTGACTTCTG TGGAGCTGAC TGAGAATCCA CTGAGAGAGC TTCAAGCTGT GAAGACATTA 
AAGCCAGATC TGGCCATCAT ACATTCAAAA TTGGGCACAC ATCCTCAGCC TCTGAAGGGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599202.2
CDS	30..2672
Translation	MDAAGASCSSSEAVARELLLAALQDLSQEQLKRFRHKLRDAPLDGRSIPWGRLEHSDAVDLTDKLIEFYAPEPAVDVTRKILKKADIRDVSLRLKEQQLQRLGSSSALLTVSEYKKKYREHVLRQHAKVKERNARSVKINKRFTKLLIAPGSGAGEDELLGTSGEPEPERARRSDTHTFNRLFRGNDDEGPRPLTVVLQGPAGIGKTMAAKKILYDWAGGKLYHSQVDFAFFMPCGELLERPGTRSLADLILEQCPDRTAPVRRILAQPHRLLFILDGADELPTLAAPEATPCRDPFEATSGLRVLSGLLSQELLPAARLLVTSRNATLGRLQGRLCSPQCAEVRGFSDKDKKKYFFKFFRDERKAERAYRFVKENETLYALCFVPFVCWIVCTVLLQQMELGRDLSRTSKTTTSVYLLFITSMLKSAGTNGPRVQGELRMLCRLAREGILKHQAQFSEKDLERLKLQGSQVQTMFLSKKELPGVLETVVTYQFIDQSFQEFLAALSYLLDAEGAPGNSAGSVQMLLNSDAGLRGHLALTTRFLFGLLSTERIRDIGNHFGCVVPGRVKQDTLRWVQGQSQPKVATVGAEKKDELKDEEAEEEEEEEEEEEELNFGLELLYCLYETQEDDFVRQALSSLPEMVLERVRLTRMDLEVLSYCVQCCPDGQALRLVSCGLVAAKEKKKKKKSFMNRLKGSQSTGKQPPASLLRPLCEAMITQQCGLSILTLSHCKLPDAVCRDLSEALKVAPSLRELGLLQNRLTEAGLRLLSQGLAWPKCKVQTLRIQMPGLQEVIHYLVIVLQQSPVLTTLDLSGCQLPGTVVEPLCSALKHPKCGLKTLSLTSVELTENPLRELQAVKTLKPDLAIIHSKLGTHPQPLKG

Target ORF information:

RefSeq Version	NM_134375.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NLR family, pyrin domain containing 6 (Nlrp6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134375.3

ORF Insert Sequence:

ATGGATGCCG CTGGAGCCTC CTGCTCCAGC TCGGAGGCGG TGGCAAGGGA ACTGCTTCTG 
GCCGCACTGC AGGATCTGAG CCAGGAGCAG CTGAAGCGTT TTCGCCACAA GCTCCGGGAT 
GCGCCCCTGG ATGGCCGCAG CATCCCGTGG GGGCGACTGG AGCACTCAGA CGCTGTGGAC 
CTCACAGACA AGCTGATTGA GTTCTATGCG CCAGAGCCAG CCGTAGATGT GACCCGAAAG 
ATCCTGAAGA AGGCGGATAT TCGAGATGTG TCGTTGCGAC TCAAGGAGCA GCAGCTGCAG 
AGGCTCGGCT CCAGCTCGGC GCTTCTCACC GTGTCGGAGT ACAAGAAGAA GTACCGCGAG 
CACGTGCTAC GACAGCATGC TAAGGTGAAG GAGAGGAATG CCCGCTCCGT GAAGATCAAC 
AAGCGCTTCA CCAAGCTGCT CATTGCGCCG GGGAGCGGCG CCGGGGAGGA CGAGCTGCTG 
GGAACGTCGG GGGAGCCCGA GCCCGAGCGC GCAAGGCGCT CGGACACACA CACTTTCAAT 
CGGCTCTTCC GAGGAAATGA TGACGAGGGC CCGCGGCCGC TGACCGTGGT GCTGCAGGGC 
CCGGCAGGCA TCGGCAAAAC CATGGCCGCC AAGAAAATCC TCTACGACTG GGCAGGAGGC 
AAGCTATACC ACAGCCAAGT GGACTTTGCC TTCTTCATGC CGTGCGGCGA GCTGCTGGAG 
CGGCCAGGTA CGCGCAGCTT GGCGGACCTG ATACTGGAAC AGTGCCCGGA CCGCACCGCG 
CCGGTGCGGC GGATACTAGC GCAACCGCAT CGTCTACTGT TCATCCTGGA CGGTGCAGAC 
GAATTGCCTA CTTTAGCAGC CCCAGAAGCC ACTCCCTGCA GAGACCCTTT CGAGGCTACG 
AGTGGCTTGA GAGTGCTGAG CGGGCTGCTG AGCCAGGAGC TGTTACCAGC GGCTCGCTTG 
CTGGTGACTT CACGCAACGC CACCCTCGGG AGGCTGCAGG GCAGACTTTG CTCACCACAG 
TGCGCAGAAG TGCGCGGCTT CTCAGACAAA GACAAGAAAA AGTATTTCTT CAAGTTTTTC 
CGGGACGAGA GGAAGGCAGA GCGCGCTTAC CGCTTCGTGA AAGAGAATGA GACGCTGTAC 
GCACTGTGCT TTGTGCCGTT TGTGTGCTGG ATCGTGTGCA CCGTGCTGCT GCAGCAAATG 
GAGCTGGGCC GGGATCTGTC TCGTACCTCC AAGACCACTA CATCTGTGTA CCTGCTCTTC 
ATCACCAGCA TGCTGAAGTC TGCAGGTACC AATGGACCCC GGGTTCAGGG AGAGCTGCGA 
ATGCTGTGCC GCCTGGCCCG GGAGGGCATC CTGAAGCATC AAGCACAGTT CTCAGAAAAG 
GACCTGGAGA GATTGAAGCT TCAGGGTTCC CAAGTTCAGA CAATGTTTCT CAGCAAGAAG 
GAGCTGCCAG GAGTGCTAGA AACTGTGGTC ACCTACCAGT TCATTGACCA GAGCTTCCAG 
GAGTTCTTGG CTGCATTGTC ATACCTACTA GACGCTGAGG GAGCCCCAGG GAACTCCGCA 
GGAAGTGTGC AGATGCTCCT GAACTCTGAC GCGGGGCTGC GTGGTCATCT GGCACTCACC 
ACTAGATTCC TCTTTGGACT GCTAAGTACA GAGAGGATTC GTGACATTGG AAACCATTTT 
GGCTGTGTGG TGCCAGGGCG TGTGAAACAG GACACCTTGC GGTGGGTACA AGGACAAAGC 
CAACCCAAGG TGGCGACAGT AGGAGCAGAA AAGAAGGATG AGCTGAAGGA CGAGGAAGCA 
GAGGAGGAGG AGGAGGAGGA AGAAGAGGAG GAGGAACTCA ACTTTGGACT GGAGCTGTTG 
TACTGCCTGT ATGAGACACA AGAGGATGAT TTTGTTCGCC AGGCTCTCAG CAGCCTTCCA 
GAGATGGTAC TGGAGCGAGT TAGGCTGACC CGCATGGACC TTGAGGTTCT GAGCTACTGC 
GTGCAGTGCT GCCCGGACGG CCAGGCTCTG AGACTGGTGA GCTGTGGACT GGTGGCGGCA 
AAGGAGAAGA AGAAGAAGAA GAAGAGCTTC ATGAACCGGC TGAAGGGTTC TCAAAGCACC 
GGGAAACAAC CCCCAGCCTC CTTGCTGCGT CCACTCTGTG AGGCAATGAT TACCCAGCAA 
TGTGGTCTGA GTATTCTGAC CTTGTCACAC TGCAAACTCC CTGATGCAGT TTGTCGAGAC 
CTTTCTGAGG CTCTGAAGGT AGCTCCTTCC CTAAGGGAGC TGGGCCTCCT CCAGAACCGA 
CTCACTGAGG CAGGCCTGCG TTTACTAAGC CAAGGCCTGG CTTGGCCCAA ATGCAAGGTG 
CAGACACTCA GGATACAGAT GCCTGGCCTC CAAGAGGTGA TCCACTACCT GGTCATTGTG 
CTCCAGCAGA GCCCAGTCCT AACCACTCTG GACCTCAGTG GCTGTCAGCT GCCTGGCACT 
GTGGTGGAAC CTCTGTGTTC AGCCCTGAAG CACCCTAAAT GTGGCCTAAA GACCCTCAGT 
CTGACTTCTG TGGAGCTGAC TGAGAATCCA CTGAGAGAGC TTCAAGCTGT GAAGACATTA 
AAGCCAGATC TGGCCATCAT ACATTCAAAA TTGGGCACAC ATCCTCAGCC TCTGAAGGGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NLRP6 inflammasome orchestrates the colonic host-microbial interface by regulating goblet cell mucus secretion.
Cell156(5)1045-59(2014 Feb)
Wlodarska M,Thaiss CA,Nowarski R,Henao-Mejia J,Zhang JP,Brown EM,Frankel G,Levy M,Katz MN,Philbrick WM,Elinav E,Finlay BB,Flavell RA

Microbiota-induced activation of epithelial IL-6 signaling links inflammasome-driven inflammation with transmissible cancer.
Proceedings of the National Academy of Sciences of the United States of America110(24)9862-7(2013 Jun)
Hu B,Elinav E,Huber S,Strowig T,Hao L,Hafemann A,Jin C,Wunderlich C,Wunderlich T,Eisenbarth SC,Flavell RA

IL-22BP is regulated by the inflammasome and modulates tumorigenesis in the intestine.
Nature491(7423)259-63(2012 Nov)
Huber S,Gagliani N,Zenewicz LA,Huber FJ,Bosurgi L,Hu B,Hedl M,Zhang W,O'Connor W Jr,Murphy AJ,Valenzuela DM,Yancopoulos GD,Booth CJ,Cho JH,Ouyang W,Abraham C,Flavell RA

NLRP6 negatively regulates innate immunity and host defence against bacterial pathogens.
Nature488(7411)389-93(2012 Aug)
Anand PK,Malireddi RK,Lukens JR,Vogel P,Bertin J,Lamkanfi M,Kanneganti TD

Nod-like receptor pyrin domain-containing protein 6 (NLRP6) controls epithelial self-renewal and colorectal carcinogenesis upon injury.
Proceedings of the National Academy of Sciences of the United States of America108(23)9601-6(2011 Jun)
Normand S,Delanoye-Crespin A,Bressenot A,Huot L,Grandjean T,Peyrin-Biroulet L,Lemoine Y,Hot D,Chamaillard M

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Nlrp6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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