Slc12a5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc12a5
Entrez Gene ID	171373
Full Name	solute carrier family 12 member 5
Synonyms	Kcc2
General protein information	Preferred Names solute carrier family 12 member 5 Names solute carrier family 12 member 5 K-Cl cotransporter 2 electroneutral potassium-chloride cotransporter 2 furosemide-sensitive K-Cl cotransporter neuronal K-Cl cotransporter neuronal-specific K-Cl cotransporter rKCC2 solute carrier family 12 (potassium-chloride transporter), member 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q42
Summary	neuronal specific potassium-chloride cotransporter isoform; may play a role in response to neuronal injury [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_134363.1	NP_599190.1	solute carrier family 12 member 5

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SLC12A5	XP_001236722.3
	SLC12A5	XP_001104798.1
Bos taurus (cattle)	SLC12A5	NP_001193309.1
Pan troglodytes (chimpanzee)	SLC12A5	XP_003317037.1
Rattus norvegicus (Norway rat)	Slc12a5	NP_599190.1
Danio rerio (zebrafish)	slc12a5b	XP_003199160.2
Danio rerio (zebrafish)	LOC572215	XP_701000.5
Oryza sativa (rice)	Os08g0323700	NP_001061543.1
Homo sapiens (human)	SLC12A5	NP_065759.1
Canis lupus familiaris (dog)	SLC12A5	XP_005635409.1
Mus musculus (house mouse)	Slc12a5	NP_065066.2
Arabidopsis thaliana (thale cress)	CCC1	NP_174333.2
Xenopus tropicalis (tropical clawed frog)	slc12a5	NP_001072306.2

Gene Ontology
Bibliography

Related articles in PubMed

Increase of KCC2 in hippocampal synaptic plasticity disturbances after perinatal ethanol exposure.
Silvestre de Ferron B, Vilpoux C, Kervern M, Robert A, Antol J, Naassila M, Pierrefiche O
Addiction biology22(6)1870-1882(2017 Nov)

Neuronal Chloride Regulation via KCC2 Is Modulated through a GABA
Wright R, Newey SE, Ilie A, Wefelmeyer W, Raimondo JV, Ginham R, Mcllhinney RAJ, Akerman CJ
The Journal of neuroscience : the official journal of the Society for Neuroscience37(22)5447-5462(2017 May)

Pulsed Radiofrequency Attenuates Complete Freund's Adjuvant-Induced Epigenetic Suppression of Potassium Chloride Cotransporter 2 Expression.
Liu CK, Liao WT, Chu YC, Yang CH, Chen KH, Wu CH, Lin CR
Pain medicine (Malden, Mass.)18(4)807-813(2017 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We demonstrate that GABABR activation can regulate KCC2 at the cell surface in a manner that alters intracellular chloride and the reversal potential for the GABAAR. Our data therefore support an additional mechanism by which GABABRs are able to modulate fast synaptic inhibition.
Title: Neuronal Chloride Regulation via KCC2 Is Modulated through a GABA

Early IGF-1 primes visual cortex maturation and accelerates developmental switch between NKCC1 and KCC2 chloride transporters in enriched animals. Early enrichment and IGF-1 administration produced a decrease in the ratio between NKCC1 and KCC2 cation/chloride transporters, suggesting that enrichment, via IGF-1, may hasten the developmental switch of neuronal GABAergic responses from excitation to inhibition.
Title: Early IGF-1 primes visual cortex maturation and accelerates developmental switch between NKCC1 and KCC2 chloride transporters in enriched animals.

findings suggested that melatonin restores KCC2 expression, inhibits neuronal apoptosis and attenuates secondary brain injury after TBI, partially through activation of BDNF/ERK pathway.
Title: Melatonin attenuates neuronal apoptosis through up-regulation of K(+) -Cl(-) cotransporter KCC2 expression following traumatic brain injury in rats.

Findings suggest that aberrant regulation of the plasma membrane levels of KCC2 might contribute to the impartment of GABAergic neurotransmission and that KCC2 dysfunction might be relevant for the initial appearance of spontaneous seizures.
Title: Regulation of the cell surface expression of chloride transporters during epileptogenesis.

This study demonstrate that KCC2 plays a critical role in the regulation of spine actin cytoskeleton and gates long-term plasticity at excitatory synapses in cortical neurons.
Title: KCC2 Gates Activity-Driven AMPA Receptor Traffic through Cofilin Phosphorylation.

The following Slc12a5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc12a5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13550	NM_134363.1 Latest version!	Rattus norvegicus solute carrier family 12 member 5 (Slc12a5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc12a5 ( NM_134363.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_599190.1 Rattus norvegicus solute carrier family 12 member 5 (Slc12a5), mRNA.

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CloneID	ORa13550
Clone ID Related Accession (Same CDS sequence)	NM_134363.1
Accession Version	NM_134363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U55816.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U55816.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCTCAACA ACCTGACGGA CTGCGAGGAC GGCGATGGGG GAGCCAACCC GGGTGACGGC 
AATCCCAAGG AGAGCAGCCC CTTCATCAAC AGCACGGACA CGGAGAAGGG GAGAGAGTAT 
GATGGCAGGA ACATGGCCCT GTTTGAGGAG GAGATGGACA CCAGCCCCAT GGTATCCTCC 
CTGCTCAGTG GGCTGGCCAA CTACACCAAC CTGCCTCAGG GAAGCAAAGA GCACGAAGAA 
GCAGAAAACA ATGAGGGCGG AAAGAAGAAG CCGGTGCAGG CCCCACGCAT GGGCACCTTC 
ATGGGCGTGT ACCTCCCGTG CCTGCAGAAC ATCTTTGGTG TTATCCTCTT TCTGCGGCTC 
ACTTGGGTGG TGGGAATCGC AGGCATCATG GAGTCCTTCT GCATGGTCTT CATCTGCTGC 
TCCTGCACGA TGCTCACAGC CATTTCCATG AGCGCAATTG CAACCAATGG TGTTGTGCCT 
GCTGGTGGCT CCTACTACAT GATTTCCAGG TCTCTGGGCC CGGAGTTTGG GGGCGCCGTG 
GGCCTCTGCT TCTACCTGGG CACTACCTTT GCTGGGGCTA TGTACATCCT GGGCACCATC 
GAGATCCTGC TGGCTTACCT CTTCCCAGCG ATGGCCATCT TCAAGGCAGA AGATGCCAGT 
GGGGAGGCAG CCGCCATGTT GAATAACATG CGGGTGTATG GCACCTGTGT GCTCACCTGC 
ATGGCCACCG TAGTCTTTGT GGGCGTCAAG TACGTGAACA AGTTTGCCCT GGTCTTCCTG 
GGTTGCGTGA TCCTCTCCAT CCTGGCCATC TACGCAGGGG TCATCAAGTC TGCCTTCGAT 
CCACCCAATT TCCCGATTTG CCTCCTGGGG AACCGCACGC TGTCTCGCCA TGGCTTTGAT 
GTCTGTGCCA AGCTGGCTTG GGAAGGAAAT GAGACAGTGA CCACACGGCT CTGGGGCCTA 
TTCTGTTCCT CCCGCCTCCT CAATGCCACC TGTGATGAGT ACTTCACCCG AAACAATGTC 
ACAGAGATCC AGGGCATTCC TGGTGCTGCA AGTGGCCTCA TCAAAGAGAA CCTGTGGAGT 
TCCTACCTGA CCAAGGGGGT GATCGTGGAG AGGCGTGGGA TGCCCTCTGT GGGCCTGGCA 
GATGGTACCC CCGTTGACAT GGACCACCCC TATGTCTTCA GTGATATGAC CTCCTACTTC 
ACCCTGCTTG TTGGCATCTA TTTCCCCTCA GTCACAGGGA TCATGGCTGG CTCGAACCGG 
TCCGGAGACC TGCGGGATGC CCAGAAGTCT ATCCCTACTG GAACTATCTT GGCCATTGCT 
ACGACCTCTG CTGTCTACAT CAGCTCTGTT GTTCTGTTCG GAGCCTGCAT CGAAGGGGTC 
GTCCTACGGG ACAAGTTTGG GGAAGCTGTG AATGGCAATC TGGTGGTGGG CACCCTGGCC 
TGGCCTTCTC CTTGGGTCAT TGTCATAGGC TCTTTCTTCT CTACCTGCGG AGCTGGACTA 
CAGAGCCTCA CAGGGGCCCC ACGCCTGCTG CAGGCCATCT CCCGGGATGG CATAGTGCCC 
TTCCTGCAGG TCTTTGGCCA TGGCAAAGCC AACGGAGAGC CAACCTGGGC GCTGCTGCTG 
ACTGCCTGCA TCTGTGAGAT CGGCATCCTC ATCGCCTCCC TGGATGAGGT CGCCCCTATC 
CTTTCCATGT TCTTCCTGAT GTGTTACATG TTTGTGAACT TGGCTTGCGC GGTGCAGACA 
CTGCTGAGGA CGCCCAACTG GAGGCCACGC TTCCGATATT ACCACTGGAC CCTCTCCTTC 
CTGGGCATGA GCCTCTGCCT GGCCCTGATG TTCATTTGCT CCTGGTATTA TGCGCTGGTA 
GCTATGCTCA TCGCTGGCCT CATCTATAAG TACATCGAGT ACCGGGGGGC AGAGAAGGAG 
TGGGGGGATG GGATCCGAGG CCTGTCTCTC AGTGCAGCTC GCTATGCTCT CTTGCGTCTG 
GAGGAAGGAC CCCCGCATAC AAAGAACTGG AGGCCCCAGC TACTGGTGCT GGTGCGTGTG 
GACCAGGACC AGAACGTGGT GCACCCGCAG CTGCTGTCCT TGACCTCCCA GCTCAAGGCA 
GGGAAGGGCC TGACCATTGT GGGCTCTGTC CTTGAGGGCA CCTTTCTGGA CAACCACCCT 
CAGGCTCAGC GGGCAGAGGA GTCTATCCGG CGCCTGATGG AGGCTGAGAA GGTGAAGGGC 
TTCTGCCAGG TAGTGATCTC CTCCAACCTG CGTGACGGTG TGTCCCACCT GATCCAATCC 
GGGGGCCTCG GGGGCCTGCA ACACAACACT GTGCTAGTGG GCTGGCCTCG CAACTGGCGA 
CAGAAGGAGG ATCATCAGAC ATGGAGGAAC TTCATCGAAC TCGTCCGGGA AACTACAGCT 
GGCCACCTCG CCCTGCTGGT CACCAAGAAT GTTTCCATGT TCCCCGGGAA CCCTGAGCGT 
TTCTCTGAGG GCAGCATTGA CGTGTGGTGG ATCGTGCACG ACGGGGGCAT GCTCATGCTG 
TTGCCCTTCC TCCTGCGTCA CCACAAGGTC TGGAGGAAAT GCAAAATGCG GATCTTCACC 
GTGGCGCAGA TGGATGACAA CAGCATTCAG ATGAAGAAAG ACCTGACCAC GTTTCTGTAC 
CACTTACGAA TTACTGCAGA GGTGGAAGTC GTGGAGATGC ACGAGAGCGA CATCTCAGCA 
TACACCTACG AGAAGACATT GGTAATGGAA CAACGTTCTC AGATCCTCAA ACAGATGCAC 
CTCACCAAGA ACGAGCGGGA ACGGGAGATC CAGAGCATCA CAGATGAATC TCGGGGCTCC 
ATTCGGAGGA AGAATCCAGC CAACACTCGG CTCCGCCTCA ATGTTCCCGA AGAGACAGCT 
TGTGACAACG AGGAGAAGCC AGAAGAGGAG GTGCAGCTGA TCCATGACCA GAGTGCTCCC 
AGCTGCCCTA GCAGCTCGCC GTCTCCAGGG GAGGAGCCTG AGGGGGAGGG GGAGACAGAC 
CCAGAGAAGG TGCATCTCAC CTGGACCAAG GATAAGTCAG CGGCTCAGAA GAACAAAGGC 
CCCAGTCCCG TCTCCTCGGA GGGGATCAAG GACTTCTTCA GCATGAAGCC GGAGTGGGAA 
AACTTGAACC AGTCCAACGT GCGGCGCATG CACACAGCTG TGCGGCTGAA CGAGGTCATC 
GTGAATAAAT CCCGGGATGC CAAGTTGGTG TTGCTCAACA TGCCCGGGCC TCCCCGCAAC 
CGCAATGGAG ATGAAAACTA CATGGAATTC CTGGAGGTCC TCACTGAGCA ACTGGACCGG 
GTGATGCTGG TCCGCGGTGG TGGCCGAGAG GTCATCACCA TCTACTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599190.1
CDS	116..3466
Translation	MLNNLTDCEDGDGGANPGDGNPKESSPFINSTDTEKGREYDGRNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRLLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETACDNEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSAAQKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEQLDRVMLVRGGGREVITIYS

Target ORF information:

RefSeq Version	NM_134363.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 5 (Slc12a5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134363.1

ORF Insert Sequence:

ATGCTCAACA ACCTGACGGA CTGCGAGGAC GGCGATGGGG GAGCCAACCC GGGTGACGGC 
AATCCCAAGG AGAGCAGCCC CTTCATCAAC AGCACGGACA CGGAGAAGGG GAGAGAGTAT 
GATGGCAGGA ACATGGCCCT GTTTGAGGAG GAGATGGACA CCAGCCCCAT GGTATCCTCC 
CTGCTCAGTG GGCTGGCCAA CTACACCAAC CTGCCTCAGG GAAGCAAAGA GCACGAAGAA 
GCAGAAAACA ATGAGGGCGG AAAGAAGAAG CCGGTGCAGG CCCCACGCAT GGGCACCTTC 
ATGGGCGTGT ACCTCCCGTG CCTGCAGAAC ATCTTTGGTG TTATCCTCTT TCTGCGGCTC 
ACTTGGGTGG TGGGAATCGC AGGCATCATG GAGTCCTTCT GCATGGTCTT CATCTGCTGC 
TCCTGCACGA TGCTCACAGC CATTTCCATG AGCGCAATTG CAACCAATGG TGTTGTGCCT 
GCTGGTGGCT CCTACTACAT GATTTCCAGG TCTCTGGGCC CGGAGTTTGG GGGCGCCGTG 
GGCCTCTGCT TCTACCTGGG CACTACCTTT GCTGGGGCTA TGTACATCCT GGGCACCATC 
GAGATCCTGC TGGCTTACCT CTTCCCAGCG ATGGCCATCT TCAAGGCAGA AGATGCCAGT 
GGGGAGGCAG CCGCCATGTT GAATAACATG CGGGTGTATG GCACCTGTGT GCTCACCTGC 
ATGGCCACCG TAGTCTTTGT GGGCGTCAAG TACGTGAACA AGTTTGCCCT GGTCTTCCTG 
GGTTGCGTGA TCCTCTCCAT CCTGGCCATC TACGCAGGGG TCATCAAGTC TGCCTTCGAT 
CCACCCAATT TCCCGATTTG CCTCCTGGGG AACCGCACGC TGTCTCGCCA TGGCTTTGAT 
GTCTGTGCCA AGCTGGCTTG GGAAGGAAAT GAGACAGTGA CCACACGGCT CTGGGGCCTA 
TTCTGTTCCT CCCGCCTCCT CAATGCCACC TGTGATGAGT ACTTCACCCG AAACAATGTC 
ACAGAGATCC AGGGCATTCC TGGTGCTGCA AGTGGCCTCA TCAAAGAGAA CCTGTGGAGT 
TCCTACCTGA CCAAGGGGGT GATCGTGGAG AGGCGTGGGA TGCCCTCTGT GGGCCTGGCA 
GATGGTACCC CCGTTGACAT GGACCACCCC TATGTCTTCA GTGATATGAC CTCCTACTTC 
ACCCTGCTTG TTGGCATCTA TTTCCCCTCA GTCACAGGGA TCATGGCTGG CTCGAACCGG 
TCCGGAGACC TGCGGGATGC CCAGAAGTCT ATCCCTACTG GAACTATCTT GGCCATTGCT 
ACGACCTCTG CTGTCTACAT CAGCTCTGTT GTTCTGTTCG GAGCCTGCAT CGAAGGGGTC 
GTCCTACGGG ACAAGTTTGG GGAAGCTGTG AATGGCAATC TGGTGGTGGG CACCCTGGCC 
TGGCCTTCTC CTTGGGTCAT TGTCATAGGC TCTTTCTTCT CTACCTGCGG AGCTGGACTA 
CAGAGCCTCA CAGGGGCCCC ACGCCTGCTG CAGGCCATCT CCCGGGATGG CATAGTGCCC 
TTCCTGCAGG TCTTTGGCCA TGGCAAAGCC AACGGAGAGC CAACCTGGGC GCTGCTGCTG 
ACTGCCTGCA TCTGTGAGAT CGGCATCCTC ATCGCCTCCC TGGATGAGGT CGCCCCTATC 
CTTTCCATGT TCTTCCTGAT GTGTTACATG TTTGTGAACT TGGCTTGCGC GGTGCAGACA 
CTGCTGAGGA CGCCCAACTG GAGGCCACGC TTCCGATATT ACCACTGGAC CCTCTCCTTC 
CTGGGCATGA GCCTCTGCCT GGCCCTGATG TTCATTTGCT CCTGGTATTA TGCGCTGGTA 
GCTATGCTCA TCGCTGGCCT CATCTATAAG TACATCGAGT ACCGGGGGGC AGAGAAGGAG 
TGGGGGGATG GGATCCGAGG CCTGTCTCTC AGTGCAGCTC GCTATGCTCT CTTGCGTCTG 
GAGGAAGGAC CCCCGCATAC AAAGAACTGG AGGCCCCAGC TACTGGTGCT GGTGCGTGTG 
GACCAGGACC AGAACGTGGT GCACCCGCAG CTGCTGTCCT TGACCTCCCA GCTCAAGGCA 
GGGAAGGGCC TGACCATTGT GGGCTCTGTC CTTGAGGGCA CCTTTCTGGA CAACCACCCT 
CAGGCTCAGC GGGCAGAGGA GTCTATCCGG CGCCTGATGG AGGCTGAGAA GGTGAAGGGC 
TTCTGCCAGG TAGTGATCTC CTCCAACCTG CGTGACGGTG TGTCCCACCT GATCCAATCC 
GGGGGCCTCG GGGGCCTGCA ACACAACACT GTGCTAGTGG GCTGGCCTCG CAACTGGCGA 
CAGAAGGAGG ATCATCAGAC ATGGAGGAAC TTCATCGAAC TCGTCCGGGA AACTACAGCT 
GGCCACCTCG CCCTGCTGGT CACCAAGAAT GTTTCCATGT TCCCCGGGAA CCCTGAGCGT 
TTCTCTGAGG GCAGCATTGA CGTGTGGTGG ATCGTGCACG ACGGGGGCAT GCTCATGCTG 
TTGCCCTTCC TCCTGCGTCA CCACAAGGTC TGGAGGAAAT GCAAAATGCG GATCTTCACC 
GTGGCGCAGA TGGATGACAA CAGCATTCAG ATGAAGAAAG ACCTGACCAC GTTTCTGTAC 
CACTTACGAA TTACTGCAGA GGTGGAAGTC GTGGAGATGC ACGAGAGCGA CATCTCAGCA 
TACACCTACG AGAAGACATT GGTAATGGAA CAACGTTCTC AGATCCTCAA ACAGATGCAC 
CTCACCAAGA ACGAGCGGGA ACGGGAGATC CAGAGCATCA CAGATGAATC TCGGGGCTCC 
ATTCGGAGGA AGAATCCAGC CAACACTCGG CTCCGCCTCA ATGTTCCCGA AGAGACAGCT 
TGTGACAACG AGGAGAAGCC AGAAGAGGAG GTGCAGCTGA TCCATGACCA GAGTGCTCCC 
AGCTGCCCTA GCAGCTCGCC GTCTCCAGGG GAGGAGCCTG AGGGGGAGGG GGAGACAGAC 
CCAGAGAAGG TGCATCTCAC CTGGACCAAG GATAAGTCAG CGGCTCAGAA GAACAAAGGC 
CCCAGTCCCG TCTCCTCGGA GGGGATCAAG GACTTCTTCA GCATGAAGCC GGAGTGGGAA 
AACTTGAACC AGTCCAACGT GCGGCGCATG CACACAGCTG TGCGGCTGAA CGAGGTCATC 
GTGAATAAAT CCCGGGATGC CAAGTTGGTG TTGCTCAACA TGCCCGGGCC TCCCCGCAAC 
CGCAATGGAG ATGAAAACTA CATGGAATTC CTGGAGGTCC TCACTGAGCA ACTGGACCGG 
GTGATGCTGG TCCGCGGTGG TGGCCGAGAG GTCATCACCA TCTACTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increase of KCC2 in hippocampal synaptic plasticity disturbances after perinatal ethanol exposure.
Addiction biology22(6)1870-1882(2017 Nov)
Silvestre de Ferron B,Vilpoux C,Kervern M,Robert A,Antol J,Naassila M,Pierrefiche O

Neuronal Chloride Regulation via KCC2 Is Modulated through a GABA
The Journal of neuroscience : the official journal of the Society for Neuroscience37(22)5447-5462(2017 May)
Wright R,Newey SE,Ilie A,Wefelmeyer W,Raimondo JV,Ginham R,Mcllhinney RAJ,Akerman CJ

Pulsed Radiofrequency Attenuates Complete Freund's Adjuvant-Induced Epigenetic Suppression of Potassium Chloride Cotransporter 2 Expression.
Pain medicine (Malden, Mass.)18(4)807-813(2017 04)
Liu CK,Liao WT,Chu YC,Yang CH,Chen KH,Wu CH,Lin CR

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Slc12a5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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