Il12rb2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Il12rb2
Entrez Gene ID	171334
Full Name	interleukin 12 receptor subunit beta 2
General protein information	Preferred Names interleukin 12 receptor subunit beta 2 Names interleukin-12 receptor subunit beta-2 interleukin 12 receptor, beta 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q31
Summary	transmembrane protein that belongs to the hemopoietin receptor superfamily; human homolg binds interleukin (IL)12 with a low affinity and may be a part of the IL12 receptor complex [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191750.1	NP_001178679.1	interleukin-12 receptor subunit beta-2 precursor
XM_008762975.2	XP_008761197.1	interleukin-12 receptor subunit beta-2 isoform X2
XM_017592423.1	XP_017447912.1	interleukin-12 receptor subunit beta-2 isoform X3
XM_006236614.3	XP_006236676.1	interleukin-12 receptor subunit beta-2 isoform X1
XM_006236613.3	XP_006236675.1	interleukin-12 receptor subunit beta-2 isoform X1
XM_008762974.2	XP_008761196.1	interleukin-12 receptor subunit beta-2 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	IL12RB2	NP_001020570.1
Gallus gallus (chicken)	IL12RB2	NP_001264556.1
Xenopus tropicalis (tropical clawed frog)	il12rb2	XP_004914374.1
Pan troglodytes (chimpanzee)	IL12RB2	XP_001163965.1
	IL12RB2	XP_001094235.1
Bos taurus (cattle)	IL12RB2	NP_777070.1
Danio rerio (zebrafish)	LOC100332888	XP_002662524.1
Rattus norvegicus (Norway rat)	Il12rb2	NP_001178679.1
Danio rerio (zebrafish)	il12rb2	NP_001106977.1
Mus musculus (house mouse)	Il12rb2	NP_032380.1
Homo sapiens (human)	IL12RB2	NP_001550.1

Gene Ontology
Bibliography

Related articles in PubMed

NOD2 is a negative regulator of Toll-like receptor 2-mediated T helper type 1 responses.
Watanabe T, Kitani A, Murray PJ, Strober W
Nature immunology5(8)800-8(2004 Aug)

A point mutation in the IL-12R beta 2 gene underlies the IL-12 unresponsiveness of Lps-defective C57BL/10ScCr mice.
Poltorak A, Merlin T, Nielsen PJ, Sandra O, Smirnova I, Schupp I, Boehm T, Galanos C, Freudenberg MA
Journal of immunology (Baltimore, Md. : 1950)167(4)2106-11(2001 Aug)

Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.
Oppmann B, Lesley R, Blom B, Timans JC, Xu Y, Hunte B, Vega F, Yu N, Wang J, Singh K, Zonin F, Vaisberg E, Churakova T, Liu M, Gorman D, Wagner J, Zurawski S, Liu Y, Abrams JS, Moore KW, Rennick D, de Waal-Malefyt R, Hannum C, Bazan JF, Kastelein RA
Immunity13(5)715-25(2000 Nov)

Role of beta1 and beta2 subunits of the interleukin-12 receptor in determining T helper 1/T helper 2 responses in vivo in the rat.
Gillespie KM, Szeto CC, Betin VM, Mathieson PW
Immunology99(1)109-12(2000 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Il12rb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Il12rb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa44299	XM_008762975.2 Latest version!	Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa44299	XM_008762974.2 Latest version!	Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa07549	NM_001191750.1 Latest version!	Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa07549	XM_006236614.3 Latest version!	Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa07549	XM_006236613.3 Latest version!	Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa60717	XM_017592423.1 Latest version!	Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Il12rb2 ( XM_008762975.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761197.1 Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa44299
Clone ID Related Accession (Same CDS sequence)	XM_008762975.2 , XM_008762974.2
Accession Version	XM_008762975.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	interleukin-12 receptor subunit beta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACACA CTGTTAGAGG ATGCTCATTG GCACTTATTT TTTTGTTCAT GTGGCTGTTG 
ATTAAAGCAA ATATAGATGT GTGCAAGCTT GGCACCGTGA CTGTCCAGCC TGCCCCTGTG 
ATTCCACTTG GGTCAACTGC CAATATTTCC TGCTCTTTGA ATCCCAAACA AGGCTGTTCG 
CATTATCCTA GTTATAACGA ATTAATCCTC TTAAAGTTTG TCAATGATGT CCTTGTCGAA 
AAACTCCATG GCAAAAAAGT CCATGGCCAC CCTAGTCATT CCTCCATATT TCAAGTCACC 
AACCTGTCTC TTGGTATGAC TCTGTTTGTC TGCAAGCTAA ACTGTAGCAA CTCTCAAAAG 
ACACAACCAG TGCCAGTGTG TGGAGTGGAG ATCTCAGTCG GTGTTGCTCC AGAGTCACCT 
CAAAGCATAT CATGTGTCCA GAAAGGAGAG CATGGAACTG TGGCCTGCTC CTGGAACTCT 
GGGAAAGTTA CTTATCTGAA AACCTATTAC ACTTTACAGT TAACTGGACC AAACAATGTG 
ACCTATCAGA ACCAGTGTTA TTCTGACAAT CGTCAGAATT GTAGTCGTCT GGATCTTGGG 
ATCAATCTGA CCCCTGATTT AGCCGAATCC AGGTTCATAG TCCATGTTAC TGCCACCAAT 
GATCTTGGAA ATTCTTCTTC ACTTCTGCAT ACATTCACAT TCTTGGACAT AGTGAGGCCT 
CTTCCTCCGT GGGACATCAG AATCAACTTT CTAAATGCTT CTGGGAGCAG ATGTACACTG 
CAGTGGGAAG ATGAGGGGCA AGTGGTACTC AATCGACTCA GATACCAGCC TCTTAACAGC 
ACATCCTGGA ATATGGTTAA TGCTACAAAT GCCAAAGGAA GATATGACCT GCAAGATCTG 
AGACCATTCA CAGAATATGA ATTTCAGATC TCCTCTAAGC TACATCTCTC TCAGGGAAGT 
TGGAGTAATT GGAGTGAGTC ACTGAGAACT CGAACACCAG AGGAAGAGCC TGTTGGGACA 
TTAGACATCT GGTACATGAA ACAAGATATT GACTGTGACA GACAACAGAT CTCTCTTTTC 
TGGAAGAGTC TGAATCCATC AGAGGCAAGG GGGAAGATCC TCCACTATCA GGTGACTTTA 
CAGGAGGTGA CGAAGAAAAC TACACTGCAG AATACTACAA GACATACCTT CTGGACCGGG 
GTCATTCCCA GAACTGGGGC TTGGACTGCA TCTGTGTCTG CAGCCAACTC AAAAGGCACT 
TCTGCACCCA CTCACATTAA CATAGTGGAC CTGTGTGGCA CTGGGTTCCT GGCTCCTCAC 
CAGGTCTCTG CAAAGTCAGG GAACATGGAC AACATCATGG TGACCTGGCA GCCTCCTGGG 
AAAGCTGACT CTGTGATTCG GGAGTACATA GTGGAATGGA GGGCTCTCCA ACAAGGGAGC 
GTCATGAACC TTACCCCACA CTGGCTGCGA ATTCCCCCCT ACAACATGTC TGCTCTGATT 
TCAGAGAAGA TAAAGCCCTA TATCTGTTAC GAAATCCGGG TACATGCACT GTCAGAGGAC 
CAAGGAGGGT GCAGCTCCAT CCGGGGTGAC TCCAGTCACA AAGTGCCACT GAGTGGCCCT 
CATATCACTG CTATCACAGA GAAAAAGGAA AGCCTTTTTA TTTCCTGGAC CCACATCCCT 
TTCCTGGAGC AAAGGGGCTG CATCCTCCAT TACAGAATAT ATTGGAAGGA GCGACACTCT 
ACTGCGCAAC CCGAGCTCTG TGAAATTCAG TACAGACACT CTCAAAACTC ACATCCAATA 
AGCAGCCTAC AGCCCAAGGT GACATATGTC CTATGGATGA CAGCTGTGAC AGCTGCTGGT 
GAAAGTCCCC AAGGAAATGA AAGGGAATTT TGTCCACAGG GGCATTTACT CTCCTTTCTA 
CTCTCAAACC CCAATGGTAT TGCAGAACTA TTCCAGATCC AGCAAATAGC ACTTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761197.1
CDS	486..2465
Translation	MAHTVRGCSLALIFLFMWLLIKANIDVCKLGTVTVQPAPVIPLGSTANISCSLNPKQGCSHYPSYNELILLKFVNDVLVEKLHGKKVHGHPSHSSIFQVTNLSLGMTLFVCKLNCSNSQKTQPVPVCGVEISVGVAPESPQSISCVQKGEHGTVACSWNSGKVTYLKTYYTLQLTGPNNVTYQNQCYSDNRQNCSRLDLGINLTPDLAESRFIVHVTATNDLGNSSSLLHTFTFLDIVRPLPPWDIRINFLNASGSRCTLQWEDEGQVVLNRLRYQPLNSTSWNMVNATNAKGRYDLQDLRPFTEYEFQISSKLHLSQGSWSNWSESLRTRTPEEEPVGTLDIWYMKQDIDCDRQQISLFWKSLNPSEARGKILHYQVTLQEVTKKTTLQNTTRHTFWTGVIPRTGAWTASVSAANSKGTSAPTHINIVDLCGTGFLAPHQVSAKSGNMDNIMVTWQPPGKADSVIREYIVEWRALQQGSVMNLTPHWLRIPPYNMSALISEKIKPYICYEIRVHALSEDQGGCSSIRGDSSHKVPLSGPHITAITEKKESLFISWTHIPFLEQRGCILHYRIYWKERHSTAQPELCEIQYRHSQNSHPISSLQPKVTYVLWMTAVTAAGESPQGNEREFCPQGHLLSFLLSNPNGIAELFQIQQIALG

Target ORF information:

RefSeq Version	XM_008762975.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762975.2

ORF Insert Sequence:

ATGGCACACA CTGTTAGAGG ATGCTCATTG GCACTTATTT TTTTGTTCAT GTGGCTGTTG 
ATTAAAGCAA ATATAGATGT GTGCAAGCTT GGCACCGTGA CTGTCCAGCC TGCCCCTGTG 
ATTCCACTTG GGTCAACTGC CAATATTTCC TGCTCTTTGA ATCCCAAACA AGGCTGTTCG 
CATTATCCTA GTTATAACGA ATTAATCCTC TTAAAGTTTG TCAATGATGT CCTTGTCGAA 
AAACTCCATG GCAAAAAAGT CCATGGCCAC CCTAGTCATT CCTCCATATT TCAAGTCACC 
AACCTGTCTC TTGGTATGAC TCTGTTTGTC TGCAAGCTAA ACTGTAGCAA CTCTCAAAAG 
ACACAACCAG TGCCAGTGTG TGGAGTGGAG ATCTCAGTCG GTGTTGCTCC AGAGTCACCT 
CAAAGCATAT CATGTGTCCA GAAAGGAGAG CATGGAACTG TGGCCTGCTC CTGGAACTCT 
GGGAAAGTTA CTTATCTGAA AACCTATTAC ACTTTACAGT TAACTGGACC AAACAATGTG 
ACCTATCAGA ACCAGTGTTA TTCTGACAAT CGTCAGAATT GTAGTCGTCT GGATCTTGGG 
ATCAATCTGA CCCCTGATTT AGCCGAATCC AGGTTCATAG TCCATGTTAC TGCCACCAAT 
GATCTTGGAA ATTCTTCTTC ACTTCTGCAT ACATTCACAT TCTTGGACAT AGTGAGGCCT 
CTTCCTCCGT GGGACATCAG AATCAACTTT CTAAATGCTT CTGGGAGCAG ATGTACACTG 
CAGTGGGAAG ATGAGGGGCA AGTGGTACTC AATCGACTCA GATACCAGCC TCTTAACAGC 
ACATCCTGGA ATATGGTTAA TGCTACAAAT GCCAAAGGAA GATATGACCT GCAAGATCTG 
AGACCATTCA CAGAATATGA ATTTCAGATC TCCTCTAAGC TACATCTCTC TCAGGGAAGT 
TGGAGTAATT GGAGTGAGTC ACTGAGAACT CGAACACCAG AGGAAGAGCC TGTTGGGACA 
TTAGACATCT GGTACATGAA ACAAGATATT GACTGTGACA GACAACAGAT CTCTCTTTTC 
TGGAAGAGTC TGAATCCATC AGAGGCAAGG GGGAAGATCC TCCACTATCA GGTGACTTTA 
CAGGAGGTGA CGAAGAAAAC TACACTGCAG AATACTACAA GACATACCTT CTGGACCGGG 
GTCATTCCCA GAACTGGGGC TTGGACTGCA TCTGTGTCTG CAGCCAACTC AAAAGGCACT 
TCTGCACCCA CTCACATTAA CATAGTGGAC CTGTGTGGCA CTGGGTTCCT GGCTCCTCAC 
CAGGTCTCTG CAAAGTCAGG GAACATGGAC AACATCATGG TGACCTGGCA GCCTCCTGGG 
AAAGCTGACT CTGTGATTCG GGAGTACATA GTGGAATGGA GGGCTCTCCA ACAAGGGAGC 
GTCATGAACC TTACCCCACA CTGGCTGCGA ATTCCCCCCT ACAACATGTC TGCTCTGATT 
TCAGAGAAGA TAAAGCCCTA TATCTGTTAC GAAATCCGGG TACATGCACT GTCAGAGGAC 
CAAGGAGGGT GCAGCTCCAT CCGGGGTGAC TCCAGTCACA AAGTGCCACT GAGTGGCCCT 
CATATCACTG CTATCACAGA GAAAAAGGAA AGCCTTTTTA TTTCCTGGAC CCACATCCCT 
TTCCTGGAGC AAAGGGGCTG CATCCTCCAT TACAGAATAT ATTGGAAGGA GCGACACTCT 
ACTGCGCAAC CCGAGCTCTG TGAAATTCAG TACAGACACT CTCAAAACTC ACATCCAATA 
AGCAGCCTAC AGCCCAAGGT GACATATGTC CTATGGATGA CAGCTGTGAC AGCTGCTGGT 
GAAAGTCCCC AAGGAAATGA AAGGGAATTT TGTCCACAGG GGCATTTACT CTCCTTTCTA 
CTCTCAAACC CCAATGGTAT TGCAGAACTA TTCCAGATCC AGCAAATAGC ACTTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Il12rb2 ( XM_008762974.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761196.1 Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa44299
Clone ID Related Accession (Same CDS sequence)	XM_008762975.2 , XM_008762974.2
Accession Version	XM_008762974.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	interleukin-12 receptor subunit beta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACACA CTGTTAGAGG ATGCTCATTG GCACTTATTT TTTTGTTCAT GTGGCTGTTG 
ATTAAAGCAA ATATAGATGT GTGCAAGCTT GGCACCGTGA CTGTCCAGCC TGCCCCTGTG 
ATTCCACTTG GGTCAACTGC CAATATTTCC TGCTCTTTGA ATCCCAAACA AGGCTGTTCG 
CATTATCCTA GTTATAACGA ATTAATCCTC TTAAAGTTTG TCAATGATGT CCTTGTCGAA 
AAACTCCATG GCAAAAAAGT CCATGGCCAC CCTAGTCATT CCTCCATATT TCAAGTCACC 
AACCTGTCTC TTGGTATGAC TCTGTTTGTC TGCAAGCTAA ACTGTAGCAA CTCTCAAAAG 
ACACAACCAG TGCCAGTGTG TGGAGTGGAG ATCTCAGTCG GTGTTGCTCC AGAGTCACCT 
CAAAGCATAT CATGTGTCCA GAAAGGAGAG CATGGAACTG TGGCCTGCTC CTGGAACTCT 
GGGAAAGTTA CTTATCTGAA AACCTATTAC ACTTTACAGT TAACTGGACC AAACAATGTG 
ACCTATCAGA ACCAGTGTTA TTCTGACAAT CGTCAGAATT GTAGTCGTCT GGATCTTGGG 
ATCAATCTGA CCCCTGATTT AGCCGAATCC AGGTTCATAG TCCATGTTAC TGCCACCAAT 
GATCTTGGAA ATTCTTCTTC ACTTCTGCAT ACATTCACAT TCTTGGACAT AGTGAGGCCT 
CTTCCTCCGT GGGACATCAG AATCAACTTT CTAAATGCTT CTGGGAGCAG ATGTACACTG 
CAGTGGGAAG ATGAGGGGCA AGTGGTACTC AATCGACTCA GATACCAGCC TCTTAACAGC 
ACATCCTGGA ATATGGTTAA TGCTACAAAT GCCAAAGGAA GATATGACCT GCAAGATCTG 
AGACCATTCA CAGAATATGA ATTTCAGATC TCCTCTAAGC TACATCTCTC TCAGGGAAGT 
TGGAGTAATT GGAGTGAGTC ACTGAGAACT CGAACACCAG AGGAAGAGCC TGTTGGGACA 
TTAGACATCT GGTACATGAA ACAAGATATT GACTGTGACA GACAACAGAT CTCTCTTTTC 
TGGAAGAGTC TGAATCCATC AGAGGCAAGG GGGAAGATCC TCCACTATCA GGTGACTTTA 
CAGGAGGTGA CGAAGAAAAC TACACTGCAG AATACTACAA GACATACCTT CTGGACCGGG 
GTCATTCCCA GAACTGGGGC TTGGACTGCA TCTGTGTCTG CAGCCAACTC AAAAGGCACT 
TCTGCACCCA CTCACATTAA CATAGTGGAC CTGTGTGGCA CTGGGTTCCT GGCTCCTCAC 
CAGGTCTCTG CAAAGTCAGG GAACATGGAC AACATCATGG TGACCTGGCA GCCTCCTGGG 
AAAGCTGACT CTGTGATTCG GGAGTACATA GTGGAATGGA GGGCTCTCCA ACAAGGGAGC 
GTCATGAACC TTACCCCACA CTGGCTGCGA ATTCCCCCCT ACAACATGTC TGCTCTGATT 
TCAGAGAAGA TAAAGCCCTA TATCTGTTAC GAAATCCGGG TACATGCACT GTCAGAGGAC 
CAAGGAGGGT GCAGCTCCAT CCGGGGTGAC TCCAGTCACA AAGTGCCACT GAGTGGCCCT 
CATATCACTG CTATCACAGA GAAAAAGGAA AGCCTTTTTA TTTCCTGGAC CCACATCCCT 
TTCCTGGAGC AAAGGGGCTG CATCCTCCAT TACAGAATAT ATTGGAAGGA GCGACACTCT 
ACTGCGCAAC CCGAGCTCTG TGAAATTCAG TACAGACACT CTCAAAACTC ACATCCAATA 
AGCAGCCTAC AGCCCAAGGT GACATATGTC CTATGGATGA CAGCTGTGAC AGCTGCTGGT 
GAAAGTCCCC AAGGAAATGA AAGGGAATTT TGTCCACAGG GGCATTTACT CTCCTTTCTA 
CTCTCAAACC CCAATGGTAT TGCAGAACTA TTCCAGATCC AGCAAATAGC ACTTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761196.1
CDS	485..2464
Translation	MAHTVRGCSLALIFLFMWLLIKANIDVCKLGTVTVQPAPVIPLGSTANISCSLNPKQGCSHYPSYNELILLKFVNDVLVEKLHGKKVHGHPSHSSIFQVTNLSLGMTLFVCKLNCSNSQKTQPVPVCGVEISVGVAPESPQSISCVQKGEHGTVACSWNSGKVTYLKTYYTLQLTGPNNVTYQNQCYSDNRQNCSRLDLGINLTPDLAESRFIVHVTATNDLGNSSSLLHTFTFLDIVRPLPPWDIRINFLNASGSRCTLQWEDEGQVVLNRLRYQPLNSTSWNMVNATNAKGRYDLQDLRPFTEYEFQISSKLHLSQGSWSNWSESLRTRTPEEEPVGTLDIWYMKQDIDCDRQQISLFWKSLNPSEARGKILHYQVTLQEVTKKTTLQNTTRHTFWTGVIPRTGAWTASVSAANSKGTSAPTHINIVDLCGTGFLAPHQVSAKSGNMDNIMVTWQPPGKADSVIREYIVEWRALQQGSVMNLTPHWLRIPPYNMSALISEKIKPYICYEIRVHALSEDQGGCSSIRGDSSHKVPLSGPHITAITEKKESLFISWTHIPFLEQRGCILHYRIYWKERHSTAQPELCEIQYRHSQNSHPISSLQPKVTYVLWMTAVTAAGESPQGNEREFCPQGHLLSFLLSNPNGIAELFQIQQIALG

Target ORF information:

RefSeq Version	XM_008762974.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762974.2

ORF Insert Sequence:

ATGGCACACA CTGTTAGAGG ATGCTCATTG GCACTTATTT TTTTGTTCAT GTGGCTGTTG 
ATTAAAGCAA ATATAGATGT GTGCAAGCTT GGCACCGTGA CTGTCCAGCC TGCCCCTGTG 
ATTCCACTTG GGTCAACTGC CAATATTTCC TGCTCTTTGA ATCCCAAACA AGGCTGTTCG 
CATTATCCTA GTTATAACGA ATTAATCCTC TTAAAGTTTG TCAATGATGT CCTTGTCGAA 
AAACTCCATG GCAAAAAAGT CCATGGCCAC CCTAGTCATT CCTCCATATT TCAAGTCACC 
AACCTGTCTC TTGGTATGAC TCTGTTTGTC TGCAAGCTAA ACTGTAGCAA CTCTCAAAAG 
ACACAACCAG TGCCAGTGTG TGGAGTGGAG ATCTCAGTCG GTGTTGCTCC AGAGTCACCT 
CAAAGCATAT CATGTGTCCA GAAAGGAGAG CATGGAACTG TGGCCTGCTC CTGGAACTCT 
GGGAAAGTTA CTTATCTGAA AACCTATTAC ACTTTACAGT TAACTGGACC AAACAATGTG 
ACCTATCAGA ACCAGTGTTA TTCTGACAAT CGTCAGAATT GTAGTCGTCT GGATCTTGGG 
ATCAATCTGA CCCCTGATTT AGCCGAATCC AGGTTCATAG TCCATGTTAC TGCCACCAAT 
GATCTTGGAA ATTCTTCTTC ACTTCTGCAT ACATTCACAT TCTTGGACAT AGTGAGGCCT 
CTTCCTCCGT GGGACATCAG AATCAACTTT CTAAATGCTT CTGGGAGCAG ATGTACACTG 
CAGTGGGAAG ATGAGGGGCA AGTGGTACTC AATCGACTCA GATACCAGCC TCTTAACAGC 
ACATCCTGGA ATATGGTTAA TGCTACAAAT GCCAAAGGAA GATATGACCT GCAAGATCTG 
AGACCATTCA CAGAATATGA ATTTCAGATC TCCTCTAAGC TACATCTCTC TCAGGGAAGT 
TGGAGTAATT GGAGTGAGTC ACTGAGAACT CGAACACCAG AGGAAGAGCC TGTTGGGACA 
TTAGACATCT GGTACATGAA ACAAGATATT GACTGTGACA GACAACAGAT CTCTCTTTTC 
TGGAAGAGTC TGAATCCATC AGAGGCAAGG GGGAAGATCC TCCACTATCA GGTGACTTTA 
CAGGAGGTGA CGAAGAAAAC TACACTGCAG AATACTACAA GACATACCTT CTGGACCGGG 
GTCATTCCCA GAACTGGGGC TTGGACTGCA TCTGTGTCTG CAGCCAACTC AAAAGGCACT 
TCTGCACCCA CTCACATTAA CATAGTGGAC CTGTGTGGCA CTGGGTTCCT GGCTCCTCAC 
CAGGTCTCTG CAAAGTCAGG GAACATGGAC AACATCATGG TGACCTGGCA GCCTCCTGGG 
AAAGCTGACT CTGTGATTCG GGAGTACATA GTGGAATGGA GGGCTCTCCA ACAAGGGAGC 
GTCATGAACC TTACCCCACA CTGGCTGCGA ATTCCCCCCT ACAACATGTC TGCTCTGATT 
TCAGAGAAGA TAAAGCCCTA TATCTGTTAC GAAATCCGGG TACATGCACT GTCAGAGGAC 
CAAGGAGGGT GCAGCTCCAT CCGGGGTGAC TCCAGTCACA AAGTGCCACT GAGTGGCCCT 
CATATCACTG CTATCACAGA GAAAAAGGAA AGCCTTTTTA TTTCCTGGAC CCACATCCCT 
TTCCTGGAGC AAAGGGGCTG CATCCTCCAT TACAGAATAT ATTGGAAGGA GCGACACTCT 
ACTGCGCAAC CCGAGCTCTG TGAAATTCAG TACAGACACT CTCAAAACTC ACATCCAATA 
AGCAGCCTAC AGCCCAAGGT GACATATGTC CTATGGATGA CAGCTGTGAC AGCTGCTGGT 
GAAAGTCCCC AAGGAAATGA AAGGGAATTT TGTCCACAGG GGCATTTACT CTCCTTTCTA 
CTCTCAAACC CCAATGGTAT TGCAGAACTA TTCCAGATCC AGCAAATAGC ACTTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Il12rb2 ( NM_001191750.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178679.1 Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), mRNA.

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CloneID	ORa07549
Clone ID Related Accession (Same CDS sequence)	NM_001191750.1 , XM_006236614.3 , XM_006236613.3
Accession Version	NM_001191750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	interleukin-12 receptor subunit beta-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07060865.1. On Jul 17, 2010 this sequence version replaced XM_231873.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689599, SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGGCACACA CTGTTAGAGG ATGCTCATTG GCACTTATTT TTTTGTTCAT GTGGCTGTTG 
ATTAAAGCAA ATATAGATGT GTGCAAGCTT GGCACCGTGA CTGTCCAGCC TGCCCCTGTG 
ATTCCACTTG GGTCAACTGC CAATATTTCC TGCTCTTTGA ATCCCAAACA AGGCTGTTCG 
CATTATCCTA GTTATAACGA ATTAATCCTC TTAAAGTTTG TCAATGATGT CCTTGTCGAA 
AAACTCCATG GCAAAAAAGT CCATGGCCAC CCTAGTCATT CCTCCATATT TCAAGTCACC 
AACCTGTCTC TTGGTATGAC TCTGTTTGTC TGCAAGCTAA ACTGTAGCAA CTCTCAAAAG 
ACACAACCAG TGCCAGTGTG TGGAGTGGAG ATCTCAGTCG GTGTTGCTCC AGAGTCACCT 
CAAAGCATAT CATGTGTCCA GAAAGGAGAG CATGGAACTG TGGCCTGCTC CTGGAACTCT 
GGGAAAGTTA CTTATCTGAA AACCTATTAC ACTTTACAGT TAACTGGACC AAACAATGTG 
ACCTATCAGA ACCAGTGTTA TTCTGACAAT CGTCAGAATT GTAGTCGTCT GGATCTTGGG 
ATCAATCTGA CCCCTGATTT AGCCGAATCC AGGTTCATAG TCCATGTTAC TGCCACCAAT 
GATCTTGGAA ATTCTTCTTC ACTTCTGCAT ACATTCACAT TCTTGGACAT AGTGAGGCCT 
CTTCCTCCGT GGGACATCAG AATCAACTTT CTAAATGCTT CTGGGAGCAG ATGTACACTG 
CAGTGGGAAG ATGAGGGGCA AGTGGTACTC AATCGACTCA GATACCAGCC TCTTAACAGC 
ACATCCTGGA ATATGGTTAA TGCTACAAAT GCCAAAGGAA GATATGACCT GCAAGATCTG 
AGACCATTCA CAGAATATGA ATTTCAGATC TCCTCTAAGC TACATCTCTC TCAGGGAAGT 
TGGAGTAATT GGAGTGAGTC ACTGAGAACT CGAACACCAG AGGAAGAGCC TGTTGGGACA 
TTAGACATCT GGTACATGAA ACAAGATATT GACTGTGACA GACAACAGAT CTCTCTTTTC 
TGGAAGAGTC TGAATCCATC AGAGGCAAGG GGGAAGATCC TCCACTATCA GGTGACTTTA 
CAGGAGGTGA CGAAGAAAAC TACACTGCAG AATACTACAA GACATACCTT CTGGACCGGG 
GTCATTCCCA GAACTGGGGC TTGGACTGCA TCTGTGTCTG CAGCCAACTC AAAAGGCACT 
TCTGCACCCA CTCACATTAA CATAGTGGAC CTGTGTGGCA CTGGGTTCCT GGCTCCTCAC 
CAGGTCTCTG CAAAGTCAGG GAACATGGAC AACATCATGG TGACCTGGCA GCCTCCTGGG 
AAAGCTGACT CTGTGATTCG GGAGTACATA GTGGAATGGA GGGCTCTCCA ACAAGGGAGC 
GTCATGAACC TTACCCCACA CTGGCTGCGA ATTCCCCCCT ACAACATGTC TGCTCTGATT 
TCAGAGAAGA TAAAGCCCTA TATCTGTTAC GAAATCCGGG TACATGCACT GTCAGAGGAC 
CAAGGAGGGT GCAGCTCCAT CCGGGGTGAC TCCAGTCACA AAGTGCCACT GAGTGGCCCT 
CATATCACTG CTATCACAGA GAAAAAGGAA AGCCTTTTTA TTTCCTGGAC CCACATCCCT 
TTCCTGGAGC AAAGGGGCTG CATCCTCCAT TACAGAATAT ATTGGAAGGA GCGACACTCT 
ACTGCGCAAC CCGAGCTCTG TGAAATTCAG TACAGACACT CTCAAAACTC ACATCCAATA 
AGCAGCCTAC AGCCCAAGGT GACATATGTC CTATGGATGA CAGCTGTGAC AGCTGCTGGT 
GAAAGTCCCC AAGGAAATGA AAGGGAATTT TGTCCACAGG GCAAAGCCAA CTGGAAAACA 
TTCGTGATAT CAAGCATTTG CATCGCTATC ATCGTGGTGG GCACTTTCTC AATTCGTTAC 
TTCAGGCAAA AGGCATTTAC TCTCCTTTCT ACTCTCAAAC CCCAATGGTA TTGCAGAACT 
ATTCCAGATC CAGCAAATAG CACTTGGGTA AAGAAGTACC CCATTATGGA GGAGAAGATC 
CAGCCACCTA TGGACAATCT CCTGATGGCC TGGTCCGCTC CTGAAGAGCC TGAGCCCCTG 
ATCATCAATG AAGTCCTCTA CCAAATGATC CCAGTAGGCA GACAACCCTA TTGCTTCAAA 
AGAGGACAAG GGTTCCAAGG TTACTCTACC TCCAAGGAAG ACACAATATA CCTTGCCACC 
AATCCACAAA CTACAGGAAC TCTCACAGCT GAGTCGAGAC AACTAGTGAA TCTATACAAG 
GTGCTAGGAA GCAGGGACCC TGACTCAAAA CTGGGCAACC TGACCAGCCC CTTGACAGTC 
ACCCCAGTGA ACTACCTTCC TACCCATGAA GGCTATTTAC CCTCCAACAT AGAAGACCTC 
TCATCACATG AAGTTGACCC AAGTGATTCT TTAGACCTGG ACCATCAACA TATTTCTCTT 
TCCATTTTTG CATCAAGTTC TCTCCGCCCA CTCACCTTCT GTGGTGAGAG GCTGACTCTA 
GATCGGTTAA AGATGGGCTA CGAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178679.1
CDS	1..2610
Translation	MAHTVRGCSLALIFLFMWLLIKANIDVCKLGTVTVQPAPVIPLGSTANISCSLNPKQGCSHYPSYNELILLKFVNDVLVEKLHGKKVHGHPSHSSIFQVTNLSLGMTLFVCKLNCSNSQKTQPVPVCGVEISVGVAPESPQSISCVQKGEHGTVACSWNSGKVTYLKTYYTLQLTGPNNVTYQNQCYSDNRQNCSRLDLGINLTPDLAESRFIVHVTATNDLGNSSSLLHTFTFLDIVRPLPPWDIRINFLNASGSRCTLQWEDEGQVVLNRLRYQPLNSTSWNMVNATNAKGRYDLQDLRPFTEYEFQISSKLHLSQGSWSNWSESLRTRTPEEEPVGTLDIWYMKQDIDCDRQQISLFWKSLNPSEARGKILHYQVTLQEVTKKTTLQNTTRHTFWTGVIPRTGAWTASVSAANSKGTSAPTHINIVDLCGTGFLAPHQVSAKSGNMDNIMVTWQPPGKADSVIREYIVEWRALQQGSVMNLTPHWLRIPPYNMSALISEKIKPYICYEIRVHALSEDQGGCSSIRGDSSHKVPLSGPHITAITEKKESLFISWTHIPFLEQRGCILHYRIYWKERHSTAQPELCEIQYRHSQNSHPISSLQPKVTYVLWMTAVTAAGESPQGNEREFCPQGKANWKTFVISSICIAIIVVGTFSIRYFRQKAFTLLSTLKPQWYCRTIPDPANSTWVKKYPIMEEKIQPPMDNLLMAWSAPEEPEPLIINEVLYQMIPVGRQPYCFKRGQGFQGYSTSKEDTIYLATNPQTTGTLTAESRQLVNLYKVLGSRDPDSKLGNLTSPLTVTPVNYLPTHEGYLPSNIEDLSSHEVDPSDSLDLDHQHISLSIFASSSLRPLTFCGERLTLDRLKMGYEK

Target ORF information:

RefSeq Version	NM_001191750.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191750.1

ORF Insert Sequence:

ATGGCACACA CTGTTAGAGG ATGCTCATTG GCACTTATTT TTTTGTTCAT GTGGCTGTTG 
ATTAAAGCAA ATATAGATGT GTGCAAGCTT GGCACCGTGA CTGTCCAGCC TGCCCCTGTG 
ATTCCACTTG GGTCAACTGC CAATATTTCC TGCTCTTTGA ATCCCAAACA AGGCTGTTCG 
CATTATCCTA GTTATAACGA ATTAATCCTC TTAAAGTTTG TCAATGATGT CCTTGTCGAA 
AAACTCCATG GCAAAAAAGT CCATGGCCAC CCTAGTCATT CCTCCATATT TCAAGTCACC 
AACCTGTCTC TTGGTATGAC TCTGTTTGTC TGCAAGCTAA ACTGTAGCAA CTCTCAAAAG 
ACACAACCAG TGCCAGTGTG TGGAGTGGAG ATCTCAGTCG GTGTTGCTCC AGAGTCACCT 
CAAAGCATAT CATGTGTCCA GAAAGGAGAG CATGGAACTG TGGCCTGCTC CTGGAACTCT 
GGGAAAGTTA CTTATCTGAA AACCTATTAC ACTTTACAGT TAACTGGACC AAACAATGTG 
ACCTATCAGA ACCAGTGTTA TTCTGACAAT CGTCAGAATT GTAGTCGTCT GGATCTTGGG 
ATCAATCTGA CCCCTGATTT AGCCGAATCC AGGTTCATAG TCCATGTTAC TGCCACCAAT 
GATCTTGGAA ATTCTTCTTC ACTTCTGCAT ACATTCACAT TCTTGGACAT AGTGAGGCCT 
CTTCCTCCGT GGGACATCAG AATCAACTTT CTAAATGCTT CTGGGAGCAG ATGTACACTG 
CAGTGGGAAG ATGAGGGGCA AGTGGTACTC AATCGACTCA GATACCAGCC TCTTAACAGC 
ACATCCTGGA ATATGGTTAA TGCTACAAAT GCCAAAGGAA GATATGACCT GCAAGATCTG 
AGACCATTCA CAGAATATGA ATTTCAGATC TCCTCTAAGC TACATCTCTC TCAGGGAAGT 
TGGAGTAATT GGAGTGAGTC ACTGAGAACT CGAACACCAG AGGAAGAGCC TGTTGGGACA 
TTAGACATCT GGTACATGAA ACAAGATATT GACTGTGACA GACAACAGAT CTCTCTTTTC 
TGGAAGAGTC TGAATCCATC AGAGGCAAGG GGGAAGATCC TCCACTATCA GGTGACTTTA 
CAGGAGGTGA CGAAGAAAAC TACACTGCAG AATACTACAA GACATACCTT CTGGACCGGG 
GTCATTCCCA GAACTGGGGC TTGGACTGCA TCTGTGTCTG CAGCCAACTC AAAAGGCACT 
TCTGCACCCA CTCACATTAA CATAGTGGAC CTGTGTGGCA CTGGGTTCCT GGCTCCTCAC 
CAGGTCTCTG CAAAGTCAGG GAACATGGAC AACATCATGG TGACCTGGCA GCCTCCTGGG 
AAAGCTGACT CTGTGATTCG GGAGTACATA GTGGAATGGA GGGCTCTCCA ACAAGGGAGC 
GTCATGAACC TTACCCCACA CTGGCTGCGA ATTCCCCCCT ACAACATGTC TGCTCTGATT 
TCAGAGAAGA TAAAGCCCTA TATCTGTTAC GAAATCCGGG TACATGCACT GTCAGAGGAC 
CAAGGAGGGT GCAGCTCCAT CCGGGGTGAC TCCAGTCACA AAGTGCCACT GAGTGGCCCT 
CATATCACTG CTATCACAGA GAAAAAGGAA AGCCTTTTTA TTTCCTGGAC CCACATCCCT 
TTCCTGGAGC AAAGGGGCTG CATCCTCCAT TACAGAATAT ATTGGAAGGA GCGACACTCT 
ACTGCGCAAC CCGAGCTCTG TGAAATTCAG TACAGACACT CTCAAAACTC ACATCCAATA 
AGCAGCCTAC AGCCCAAGGT GACATATGTC CTATGGATGA CAGCTGTGAC AGCTGCTGGT 
GAAAGTCCCC AAGGAAATGA AAGGGAATTT TGTCCACAGG GCAAAGCCAA CTGGAAAACA 
TTCGTGATAT CAAGCATTTG CATCGCTATC ATCGTGGTGG GCACTTTCTC AATTCGTTAC 
TTCAGGCAAA AGGCATTTAC TCTCCTTTCT ACTCTCAAAC CCCAATGGTA TTGCAGAACT 
ATTCCAGATC CAGCAAATAG CACTTGGGTA AAGAAGTACC CCATTATGGA GGAGAAGATC 
CAGCCACCTA TGGACAATCT CCTGATGGCC TGGTCCGCTC CTGAAGAGCC TGAGCCCCTG 
ATCATCAATG AAGTCCTCTA CCAAATGATC CCAGTAGGCA GACAACCCTA TTGCTTCAAA 
AGAGGACAAG GGTTCCAAGG TTACTCTACC TCCAAGGAAG ACACAATATA CCTTGCCACC 
AATCCACAAA CTACAGGAAC TCTCACAGCT GAGTCGAGAC AACTAGTGAA TCTATACAAG 
GTGCTAGGAA GCAGGGACCC TGACTCAAAA CTGGGCAACC TGACCAGCCC CTTGACAGTC 
ACCCCAGTGA ACTACCTTCC TACCCATGAA GGCTATTTAC CCTCCAACAT AGAAGACCTC 
TCATCACATG AAGTTGACCC AAGTGATTCT TTAGACCTGG ACCATCAACA TATTTCTCTT 
TCCATTTTTG CATCAAGTTC TCTCCGCCCA CTCACCTTCT GTGGTGAGAG GCTGACTCTA 
GATCGGTTAA AGATGGGCTA CGAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Il12rb2 ( XM_006236614.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236676.1 Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X2, mRNA.

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CloneID	ORa07549
Clone ID Related Accession (Same CDS sequence)	NM_001191750.1 , XM_006236614.3 , XM_006236613.3
Accession Version	XM_006236614.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	interleukin-12 receptor subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236614.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACACA CTGTTAGAGG ATGCTCATTG GCACTTATTT TTTTGTTCAT GTGGCTGTTG 
ATTAAAGCAA ATATAGATGT GTGCAAGCTT GGCACCGTGA CTGTCCAGCC TGCCCCTGTG 
ATTCCACTTG GGTCAACTGC CAATATTTCC TGCTCTTTGA ATCCCAAACA AGGCTGTTCG 
CATTATCCTA GTTATAACGA ATTAATCCTC TTAAAGTTTG TCAATGATGT CCTTGTCGAA 
AAACTCCATG GCAAAAAAGT CCATGGCCAC CCTAGTCATT CCTCCATATT TCAAGTCACC 
AACCTGTCTC TTGGTATGAC TCTGTTTGTC TGCAAGCTAA ACTGTAGCAA CTCTCAAAAG 
ACACAACCAG TGCCAGTGTG TGGAGTGGAG ATCTCAGTCG GTGTTGCTCC AGAGTCACCT 
CAAAGCATAT CATGTGTCCA GAAAGGAGAG CATGGAACTG TGGCCTGCTC CTGGAACTCT 
GGGAAAGTTA CTTATCTGAA AACCTATTAC ACTTTACAGT TAACTGGACC AAACAATGTG 
ACCTATCAGA ACCAGTGTTA TTCTGACAAT CGTCAGAATT GTAGTCGTCT GGATCTTGGG 
ATCAATCTGA CCCCTGATTT AGCCGAATCC AGGTTCATAG TCCATGTTAC TGCCACCAAT 
GATCTTGGAA ATTCTTCTTC ACTTCTGCAT ACATTCACAT TCTTGGACAT AGTGAGGCCT 
CTTCCTCCGT GGGACATCAG AATCAACTTT CTAAATGCTT CTGGGAGCAG ATGTACACTG 
CAGTGGGAAG ATGAGGGGCA AGTGGTACTC AATCGACTCA GATACCAGCC TCTTAACAGC 
ACATCCTGGA ATATGGTTAA TGCTACAAAT GCCAAAGGAA GATATGACCT GCAAGATCTG 
AGACCATTCA CAGAATATGA ATTTCAGATC TCCTCTAAGC TACATCTCTC TCAGGGAAGT 
TGGAGTAATT GGAGTGAGTC ACTGAGAACT CGAACACCAG AGGAAGAGCC TGTTGGGACA 
TTAGACATCT GGTACATGAA ACAAGATATT GACTGTGACA GACAACAGAT CTCTCTTTTC 
TGGAAGAGTC TGAATCCATC AGAGGCAAGG GGGAAGATCC TCCACTATCA GGTGACTTTA 
CAGGAGGTGA CGAAGAAAAC TACACTGCAG AATACTACAA GACATACCTT CTGGACCGGG 
GTCATTCCCA GAACTGGGGC TTGGACTGCA TCTGTGTCTG CAGCCAACTC AAAAGGCACT 
TCTGCACCCA CTCACATTAA CATAGTGGAC CTGTGTGGCA CTGGGTTCCT GGCTCCTCAC 
CAGGTCTCTG CAAAGTCAGG GAACATGGAC AACATCATGG TGACCTGGCA GCCTCCTGGG 
AAAGCTGACT CTGTGATTCG GGAGTACATA GTGGAATGGA GGGCTCTCCA ACAAGGGAGC 
GTCATGAACC TTACCCCACA CTGGCTGCGA ATTCCCCCCT ACAACATGTC TGCTCTGATT 
TCAGAGAAGA TAAAGCCCTA TATCTGTTAC GAAATCCGGG TACATGCACT GTCAGAGGAC 
CAAGGAGGGT GCAGCTCCAT CCGGGGTGAC TCCAGTCACA AAGTGCCACT GAGTGGCCCT 
CATATCACTG CTATCACAGA GAAAAAGGAA AGCCTTTTTA TTTCCTGGAC CCACATCCCT 
TTCCTGGAGC AAAGGGGCTG CATCCTCCAT TACAGAATAT ATTGGAAGGA GCGACACTCT 
ACTGCGCAAC CCGAGCTCTG TGAAATTCAG TACAGACACT CTCAAAACTC ACATCCAATA 
AGCAGCCTAC AGCCCAAGGT GACATATGTC CTATGGATGA CAGCTGTGAC AGCTGCTGGT 
GAAAGTCCCC AAGGAAATGA AAGGGAATTT TGTCCACAGG GCAAAGCCAA CTGGAAAACA 
TTCGTGATAT CAAGCATTTG CATCGCTATC ATCGTGGTGG GCACTTTCTC AATTCGTTAC 
TTCAGGCAAA AGGCATTTAC TCTCCTTTCT ACTCTCAAAC CCCAATGGTA TTGCAGAACT 
ATTCCAGATC CAGCAAATAG CACTTGGGTA AAGAAGTACC CCATTATGGA GGAGAAGATC 
CAGCCACCTA TGGACAATCT CCTGATGGCC TGGTCCGCTC CTGAAGAGCC TGAGCCCCTG 
ATCATCAATG AAGTCCTCTA CCAAATGATC CCAGTAGGCA GACAACCCTA TTGCTTCAAA 
AGAGGACAAG GGTTCCAAGG TTACTCTACC TCCAAGGAAG ACACAATATA CCTTGCCACC 
AATCCACAAA CTACAGGAAC TCTCACAGCT GAGTCGAGAC AACTAGTGAA TCTATACAAG 
GTGCTAGGAA GCAGGGACCC TGACTCAAAA CTGGGCAACC TGACCAGCCC CTTGACAGTC 
ACCCCAGTGA ACTACCTTCC TACCCATGAA GGCTATTTAC CCTCCAACAT AGAAGACCTC 
TCATCACATG AAGTTGACCC AAGTGATTCT TTAGACCTGG ACCATCAACA TATTTCTCTT 
TCCATTTTTG CATCAAGTTC TCTCCGCCCA CTCACCTTCT GTGGTGAGAG GCTGACTCTA 
GATCGGTTAA AGATGGGCTA CGAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236676.1
CDS	393..3002
Translation	MAHTVRGCSLALIFLFMWLLIKANIDVCKLGTVTVQPAPVIPLGSTANISCSLNPKQGCSHYPSYNELILLKFVNDVLVEKLHGKKVHGHPSHSSIFQVTNLSLGMTLFVCKLNCSNSQKTQPVPVCGVEISVGVAPESPQSISCVQKGEHGTVACSWNSGKVTYLKTYYTLQLTGPNNVTYQNQCYSDNRQNCSRLDLGINLTPDLAESRFIVHVTATNDLGNSSSLLHTFTFLDIVRPLPPWDIRINFLNASGSRCTLQWEDEGQVVLNRLRYQPLNSTSWNMVNATNAKGRYDLQDLRPFTEYEFQISSKLHLSQGSWSNWSESLRTRTPEEEPVGTLDIWYMKQDIDCDRQQISLFWKSLNPSEARGKILHYQVTLQEVTKKTTLQNTTRHTFWTGVIPRTGAWTASVSAANSKGTSAPTHINIVDLCGTGFLAPHQVSAKSGNMDNIMVTWQPPGKADSVIREYIVEWRALQQGSVMNLTPHWLRIPPYNMSALISEKIKPYICYEIRVHALSEDQGGCSSIRGDSSHKVPLSGPHITAITEKKESLFISWTHIPFLEQRGCILHYRIYWKERHSTAQPELCEIQYRHSQNSHPISSLQPKVTYVLWMTAVTAAGESPQGNEREFCPQGKANWKTFVISSICIAIIVVGTFSIRYFRQKAFTLLSTLKPQWYCRTIPDPANSTWVKKYPIMEEKIQPPMDNLLMAWSAPEEPEPLIINEVLYQMIPVGRQPYCFKRGQGFQGYSTSKEDTIYLATNPQTTGTLTAESRQLVNLYKVLGSRDPDSKLGNLTSPLTVTPVNYLPTHEGYLPSNIEDLSSHEVDPSDSLDLDHQHISLSIFASSSLRPLTFCGERLTLDRLKMGYEK

Target ORF information:

RefSeq Version	XM_006236614.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236614.3

ORF Insert Sequence:

ATGGCACACA CTGTTAGAGG ATGCTCATTG GCACTTATTT TTTTGTTCAT GTGGCTGTTG 
ATTAAAGCAA ATATAGATGT GTGCAAGCTT GGCACCGTGA CTGTCCAGCC TGCCCCTGTG 
ATTCCACTTG GGTCAACTGC CAATATTTCC TGCTCTTTGA ATCCCAAACA AGGCTGTTCG 
CATTATCCTA GTTATAACGA ATTAATCCTC TTAAAGTTTG TCAATGATGT CCTTGTCGAA 
AAACTCCATG GCAAAAAAGT CCATGGCCAC CCTAGTCATT CCTCCATATT TCAAGTCACC 
AACCTGTCTC TTGGTATGAC TCTGTTTGTC TGCAAGCTAA ACTGTAGCAA CTCTCAAAAG 
ACACAACCAG TGCCAGTGTG TGGAGTGGAG ATCTCAGTCG GTGTTGCTCC AGAGTCACCT 
CAAAGCATAT CATGTGTCCA GAAAGGAGAG CATGGAACTG TGGCCTGCTC CTGGAACTCT 
GGGAAAGTTA CTTATCTGAA AACCTATTAC ACTTTACAGT TAACTGGACC AAACAATGTG 
ACCTATCAGA ACCAGTGTTA TTCTGACAAT CGTCAGAATT GTAGTCGTCT GGATCTTGGG 
ATCAATCTGA CCCCTGATTT AGCCGAATCC AGGTTCATAG TCCATGTTAC TGCCACCAAT 
GATCTTGGAA ATTCTTCTTC ACTTCTGCAT ACATTCACAT TCTTGGACAT AGTGAGGCCT 
CTTCCTCCGT GGGACATCAG AATCAACTTT CTAAATGCTT CTGGGAGCAG ATGTACACTG 
CAGTGGGAAG ATGAGGGGCA AGTGGTACTC AATCGACTCA GATACCAGCC TCTTAACAGC 
ACATCCTGGA ATATGGTTAA TGCTACAAAT GCCAAAGGAA GATATGACCT GCAAGATCTG 
AGACCATTCA CAGAATATGA ATTTCAGATC TCCTCTAAGC TACATCTCTC TCAGGGAAGT 
TGGAGTAATT GGAGTGAGTC ACTGAGAACT CGAACACCAG AGGAAGAGCC TGTTGGGACA 
TTAGACATCT GGTACATGAA ACAAGATATT GACTGTGACA GACAACAGAT CTCTCTTTTC 
TGGAAGAGTC TGAATCCATC AGAGGCAAGG GGGAAGATCC TCCACTATCA GGTGACTTTA 
CAGGAGGTGA CGAAGAAAAC TACACTGCAG AATACTACAA GACATACCTT CTGGACCGGG 
GTCATTCCCA GAACTGGGGC TTGGACTGCA TCTGTGTCTG CAGCCAACTC AAAAGGCACT 
TCTGCACCCA CTCACATTAA CATAGTGGAC CTGTGTGGCA CTGGGTTCCT GGCTCCTCAC 
CAGGTCTCTG CAAAGTCAGG GAACATGGAC AACATCATGG TGACCTGGCA GCCTCCTGGG 
AAAGCTGACT CTGTGATTCG GGAGTACATA GTGGAATGGA GGGCTCTCCA ACAAGGGAGC 
GTCATGAACC TTACCCCACA CTGGCTGCGA ATTCCCCCCT ACAACATGTC TGCTCTGATT 
TCAGAGAAGA TAAAGCCCTA TATCTGTTAC GAAATCCGGG TACATGCACT GTCAGAGGAC 
CAAGGAGGGT GCAGCTCCAT CCGGGGTGAC TCCAGTCACA AAGTGCCACT GAGTGGCCCT 
CATATCACTG CTATCACAGA GAAAAAGGAA AGCCTTTTTA TTTCCTGGAC CCACATCCCT 
TTCCTGGAGC AAAGGGGCTG CATCCTCCAT TACAGAATAT ATTGGAAGGA GCGACACTCT 
ACTGCGCAAC CCGAGCTCTG TGAAATTCAG TACAGACACT CTCAAAACTC ACATCCAATA 
AGCAGCCTAC AGCCCAAGGT GACATATGTC CTATGGATGA CAGCTGTGAC AGCTGCTGGT 
GAAAGTCCCC AAGGAAATGA AAGGGAATTT TGTCCACAGG GCAAAGCCAA CTGGAAAACA 
TTCGTGATAT CAAGCATTTG CATCGCTATC ATCGTGGTGG GCACTTTCTC AATTCGTTAC 
TTCAGGCAAA AGGCATTTAC TCTCCTTTCT ACTCTCAAAC CCCAATGGTA TTGCAGAACT 
ATTCCAGATC CAGCAAATAG CACTTGGGTA AAGAAGTACC CCATTATGGA GGAGAAGATC 
CAGCCACCTA TGGACAATCT CCTGATGGCC TGGTCCGCTC CTGAAGAGCC TGAGCCCCTG 
ATCATCAATG AAGTCCTCTA CCAAATGATC CCAGTAGGCA GACAACCCTA TTGCTTCAAA 
AGAGGACAAG GGTTCCAAGG TTACTCTACC TCCAAGGAAG ACACAATATA CCTTGCCACC 
AATCCACAAA CTACAGGAAC TCTCACAGCT GAGTCGAGAC AACTAGTGAA TCTATACAAG 
GTGCTAGGAA GCAGGGACCC TGACTCAAAA CTGGGCAACC TGACCAGCCC CTTGACAGTC 
ACCCCAGTGA ACTACCTTCC TACCCATGAA GGCTATTTAC CCTCCAACAT AGAAGACCTC 
TCATCACATG AAGTTGACCC AAGTGATTCT TTAGACCTGG ACCATCAACA TATTTCTCTT 
TCCATTTTTG CATCAAGTTC TCTCCGCCCA CTCACCTTCT GTGGTGAGAG GCTGACTCTA 
GATCGGTTAA AGATGGGCTA CGAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Il12rb2 ( XM_006236613.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236675.1 Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa07549
Clone ID Related Accession (Same CDS sequence)	NM_001191750.1 , XM_006236614.3 , XM_006236613.3
Accession Version	XM_006236613.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	interleukin-12 receptor subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236613.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACACA CTGTTAGAGG ATGCTCATTG GCACTTATTT TTTTGTTCAT GTGGCTGTTG 
ATTAAAGCAA ATATAGATGT GTGCAAGCTT GGCACCGTGA CTGTCCAGCC TGCCCCTGTG 
ATTCCACTTG GGTCAACTGC CAATATTTCC TGCTCTTTGA ATCCCAAACA AGGCTGTTCG 
CATTATCCTA GTTATAACGA ATTAATCCTC TTAAAGTTTG TCAATGATGT CCTTGTCGAA 
AAACTCCATG GCAAAAAAGT CCATGGCCAC CCTAGTCATT CCTCCATATT TCAAGTCACC 
AACCTGTCTC TTGGTATGAC TCTGTTTGTC TGCAAGCTAA ACTGTAGCAA CTCTCAAAAG 
ACACAACCAG TGCCAGTGTG TGGAGTGGAG ATCTCAGTCG GTGTTGCTCC AGAGTCACCT 
CAAAGCATAT CATGTGTCCA GAAAGGAGAG CATGGAACTG TGGCCTGCTC CTGGAACTCT 
GGGAAAGTTA CTTATCTGAA AACCTATTAC ACTTTACAGT TAACTGGACC AAACAATGTG 
ACCTATCAGA ACCAGTGTTA TTCTGACAAT CGTCAGAATT GTAGTCGTCT GGATCTTGGG 
ATCAATCTGA CCCCTGATTT AGCCGAATCC AGGTTCATAG TCCATGTTAC TGCCACCAAT 
GATCTTGGAA ATTCTTCTTC ACTTCTGCAT ACATTCACAT TCTTGGACAT AGTGAGGCCT 
CTTCCTCCGT GGGACATCAG AATCAACTTT CTAAATGCTT CTGGGAGCAG ATGTACACTG 
CAGTGGGAAG ATGAGGGGCA AGTGGTACTC AATCGACTCA GATACCAGCC TCTTAACAGC 
ACATCCTGGA ATATGGTTAA TGCTACAAAT GCCAAAGGAA GATATGACCT GCAAGATCTG 
AGACCATTCA CAGAATATGA ATTTCAGATC TCCTCTAAGC TACATCTCTC TCAGGGAAGT 
TGGAGTAATT GGAGTGAGTC ACTGAGAACT CGAACACCAG AGGAAGAGCC TGTTGGGACA 
TTAGACATCT GGTACATGAA ACAAGATATT GACTGTGACA GACAACAGAT CTCTCTTTTC 
TGGAAGAGTC TGAATCCATC AGAGGCAAGG GGGAAGATCC TCCACTATCA GGTGACTTTA 
CAGGAGGTGA CGAAGAAAAC TACACTGCAG AATACTACAA GACATACCTT CTGGACCGGG 
GTCATTCCCA GAACTGGGGC TTGGACTGCA TCTGTGTCTG CAGCCAACTC AAAAGGCACT 
TCTGCACCCA CTCACATTAA CATAGTGGAC CTGTGTGGCA CTGGGTTCCT GGCTCCTCAC 
CAGGTCTCTG CAAAGTCAGG GAACATGGAC AACATCATGG TGACCTGGCA GCCTCCTGGG 
AAAGCTGACT CTGTGATTCG GGAGTACATA GTGGAATGGA GGGCTCTCCA ACAAGGGAGC 
GTCATGAACC TTACCCCACA CTGGCTGCGA ATTCCCCCCT ACAACATGTC TGCTCTGATT 
TCAGAGAAGA TAAAGCCCTA TATCTGTTAC GAAATCCGGG TACATGCACT GTCAGAGGAC 
CAAGGAGGGT GCAGCTCCAT CCGGGGTGAC TCCAGTCACA AAGTGCCACT GAGTGGCCCT 
CATATCACTG CTATCACAGA GAAAAAGGAA AGCCTTTTTA TTTCCTGGAC CCACATCCCT 
TTCCTGGAGC AAAGGGGCTG CATCCTCCAT TACAGAATAT ATTGGAAGGA GCGACACTCT 
ACTGCGCAAC CCGAGCTCTG TGAAATTCAG TACAGACACT CTCAAAACTC ACATCCAATA 
AGCAGCCTAC AGCCCAAGGT GACATATGTC CTATGGATGA CAGCTGTGAC AGCTGCTGGT 
GAAAGTCCCC AAGGAAATGA AAGGGAATTT TGTCCACAGG GCAAAGCCAA CTGGAAAACA 
TTCGTGATAT CAAGCATTTG CATCGCTATC ATCGTGGTGG GCACTTTCTC AATTCGTTAC 
TTCAGGCAAA AGGCATTTAC TCTCCTTTCT ACTCTCAAAC CCCAATGGTA TTGCAGAACT 
ATTCCAGATC CAGCAAATAG CACTTGGGTA AAGAAGTACC CCATTATGGA GGAGAAGATC 
CAGCCACCTA TGGACAATCT CCTGATGGCC TGGTCCGCTC CTGAAGAGCC TGAGCCCCTG 
ATCATCAATG AAGTCCTCTA CCAAATGATC CCAGTAGGCA GACAACCCTA TTGCTTCAAA 
AGAGGACAAG GGTTCCAAGG TTACTCTACC TCCAAGGAAG ACACAATATA CCTTGCCACC 
AATCCACAAA CTACAGGAAC TCTCACAGCT GAGTCGAGAC AACTAGTGAA TCTATACAAG 
GTGCTAGGAA GCAGGGACCC TGACTCAAAA CTGGGCAACC TGACCAGCCC CTTGACAGTC 
ACCCCAGTGA ACTACCTTCC TACCCATGAA GGCTATTTAC CCTCCAACAT AGAAGACCTC 
TCATCACATG AAGTTGACCC AAGTGATTCT TTAGACCTGG ACCATCAACA TATTTCTCTT 
TCCATTTTTG CATCAAGTTC TCTCCGCCCA CTCACCTTCT GTGGTGAGAG GCTGACTCTA 
GATCGGTTAA AGATGGGCTA CGAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236675.1
CDS	397..3006
Translation	MAHTVRGCSLALIFLFMWLLIKANIDVCKLGTVTVQPAPVIPLGSTANISCSLNPKQGCSHYPSYNELILLKFVNDVLVEKLHGKKVHGHPSHSSIFQVTNLSLGMTLFVCKLNCSNSQKTQPVPVCGVEISVGVAPESPQSISCVQKGEHGTVACSWNSGKVTYLKTYYTLQLTGPNNVTYQNQCYSDNRQNCSRLDLGINLTPDLAESRFIVHVTATNDLGNSSSLLHTFTFLDIVRPLPPWDIRINFLNASGSRCTLQWEDEGQVVLNRLRYQPLNSTSWNMVNATNAKGRYDLQDLRPFTEYEFQISSKLHLSQGSWSNWSESLRTRTPEEEPVGTLDIWYMKQDIDCDRQQISLFWKSLNPSEARGKILHYQVTLQEVTKKTTLQNTTRHTFWTGVIPRTGAWTASVSAANSKGTSAPTHINIVDLCGTGFLAPHQVSAKSGNMDNIMVTWQPPGKADSVIREYIVEWRALQQGSVMNLTPHWLRIPPYNMSALISEKIKPYICYEIRVHALSEDQGGCSSIRGDSSHKVPLSGPHITAITEKKESLFISWTHIPFLEQRGCILHYRIYWKERHSTAQPELCEIQYRHSQNSHPISSLQPKVTYVLWMTAVTAAGESPQGNEREFCPQGKANWKTFVISSICIAIIVVGTFSIRYFRQKAFTLLSTLKPQWYCRTIPDPANSTWVKKYPIMEEKIQPPMDNLLMAWSAPEEPEPLIINEVLYQMIPVGRQPYCFKRGQGFQGYSTSKEDTIYLATNPQTTGTLTAESRQLVNLYKVLGSRDPDSKLGNLTSPLTVTPVNYLPTHEGYLPSNIEDLSSHEVDPSDSLDLDHQHISLSIFASSSLRPLTFCGERLTLDRLKMGYEK

Target ORF information:

RefSeq Version	XM_006236613.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236613.3

ORF Insert Sequence:

ATGGCACACA CTGTTAGAGG ATGCTCATTG GCACTTATTT TTTTGTTCAT GTGGCTGTTG 
ATTAAAGCAA ATATAGATGT GTGCAAGCTT GGCACCGTGA CTGTCCAGCC TGCCCCTGTG 
ATTCCACTTG GGTCAACTGC CAATATTTCC TGCTCTTTGA ATCCCAAACA AGGCTGTTCG 
CATTATCCTA GTTATAACGA ATTAATCCTC TTAAAGTTTG TCAATGATGT CCTTGTCGAA 
AAACTCCATG GCAAAAAAGT CCATGGCCAC CCTAGTCATT CCTCCATATT TCAAGTCACC 
AACCTGTCTC TTGGTATGAC TCTGTTTGTC TGCAAGCTAA ACTGTAGCAA CTCTCAAAAG 
ACACAACCAG TGCCAGTGTG TGGAGTGGAG ATCTCAGTCG GTGTTGCTCC AGAGTCACCT 
CAAAGCATAT CATGTGTCCA GAAAGGAGAG CATGGAACTG TGGCCTGCTC CTGGAACTCT 
GGGAAAGTTA CTTATCTGAA AACCTATTAC ACTTTACAGT TAACTGGACC AAACAATGTG 
ACCTATCAGA ACCAGTGTTA TTCTGACAAT CGTCAGAATT GTAGTCGTCT GGATCTTGGG 
ATCAATCTGA CCCCTGATTT AGCCGAATCC AGGTTCATAG TCCATGTTAC TGCCACCAAT 
GATCTTGGAA ATTCTTCTTC ACTTCTGCAT ACATTCACAT TCTTGGACAT AGTGAGGCCT 
CTTCCTCCGT GGGACATCAG AATCAACTTT CTAAATGCTT CTGGGAGCAG ATGTACACTG 
CAGTGGGAAG ATGAGGGGCA AGTGGTACTC AATCGACTCA GATACCAGCC TCTTAACAGC 
ACATCCTGGA ATATGGTTAA TGCTACAAAT GCCAAAGGAA GATATGACCT GCAAGATCTG 
AGACCATTCA CAGAATATGA ATTTCAGATC TCCTCTAAGC TACATCTCTC TCAGGGAAGT 
TGGAGTAATT GGAGTGAGTC ACTGAGAACT CGAACACCAG AGGAAGAGCC TGTTGGGACA 
TTAGACATCT GGTACATGAA ACAAGATATT GACTGTGACA GACAACAGAT CTCTCTTTTC 
TGGAAGAGTC TGAATCCATC AGAGGCAAGG GGGAAGATCC TCCACTATCA GGTGACTTTA 
CAGGAGGTGA CGAAGAAAAC TACACTGCAG AATACTACAA GACATACCTT CTGGACCGGG 
GTCATTCCCA GAACTGGGGC TTGGACTGCA TCTGTGTCTG CAGCCAACTC AAAAGGCACT 
TCTGCACCCA CTCACATTAA CATAGTGGAC CTGTGTGGCA CTGGGTTCCT GGCTCCTCAC 
CAGGTCTCTG CAAAGTCAGG GAACATGGAC AACATCATGG TGACCTGGCA GCCTCCTGGG 
AAAGCTGACT CTGTGATTCG GGAGTACATA GTGGAATGGA GGGCTCTCCA ACAAGGGAGC 
GTCATGAACC TTACCCCACA CTGGCTGCGA ATTCCCCCCT ACAACATGTC TGCTCTGATT 
TCAGAGAAGA TAAAGCCCTA TATCTGTTAC GAAATCCGGG TACATGCACT GTCAGAGGAC 
CAAGGAGGGT GCAGCTCCAT CCGGGGTGAC TCCAGTCACA AAGTGCCACT GAGTGGCCCT 
CATATCACTG CTATCACAGA GAAAAAGGAA AGCCTTTTTA TTTCCTGGAC CCACATCCCT 
TTCCTGGAGC AAAGGGGCTG CATCCTCCAT TACAGAATAT ATTGGAAGGA GCGACACTCT 
ACTGCGCAAC CCGAGCTCTG TGAAATTCAG TACAGACACT CTCAAAACTC ACATCCAATA 
AGCAGCCTAC AGCCCAAGGT GACATATGTC CTATGGATGA CAGCTGTGAC AGCTGCTGGT 
GAAAGTCCCC AAGGAAATGA AAGGGAATTT TGTCCACAGG GCAAAGCCAA CTGGAAAACA 
TTCGTGATAT CAAGCATTTG CATCGCTATC ATCGTGGTGG GCACTTTCTC AATTCGTTAC 
TTCAGGCAAA AGGCATTTAC TCTCCTTTCT ACTCTCAAAC CCCAATGGTA TTGCAGAACT 
ATTCCAGATC CAGCAAATAG CACTTGGGTA AAGAAGTACC CCATTATGGA GGAGAAGATC 
CAGCCACCTA TGGACAATCT CCTGATGGCC TGGTCCGCTC CTGAAGAGCC TGAGCCCCTG 
ATCATCAATG AAGTCCTCTA CCAAATGATC CCAGTAGGCA GACAACCCTA TTGCTTCAAA 
AGAGGACAAG GGTTCCAAGG TTACTCTACC TCCAAGGAAG ACACAATATA CCTTGCCACC 
AATCCACAAA CTACAGGAAC TCTCACAGCT GAGTCGAGAC AACTAGTGAA TCTATACAAG 
GTGCTAGGAA GCAGGGACCC TGACTCAAAA CTGGGCAACC TGACCAGCCC CTTGACAGTC 
ACCCCAGTGA ACTACCTTCC TACCCATGAA GGCTATTTAC CCTCCAACAT AGAAGACCTC 
TCATCACATG AAGTTGACCC AAGTGATTCT TTAGACCTGG ACCATCAACA TATTTCTCTT 
TCCATTTTTG CATCAAGTTC TCTCCGCCCA CTCACCTTCT GTGGTGAGAG GCTGACTCTA 
GATCGGTTAA AGATGGGCTA CGAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Il12rb2 ( XM_017592423.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447912.1 Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X5, mRNA.

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CloneID	ORa60717
Clone ID Related Accession (Same CDS sequence)	XM_017592423.1
Accession Version	XM_017592423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	interleukin-12 receptor subunit beta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACACA CTGTTAGAGG ATGCTCATTG GCACTTATTT TTTTGTTCAT GTGGCTGTTG 
ATTAAAGCAA ATATAGATGT GTGCAAGCTT GGCACCGTGA CTGTCCAGCC TGCCCCTGTG 
ATTCCACTTG GGTCAACTGC CAATATTTCC TGCTCTTTGA ATCCCAAACA AGGCTGTTCG 
CATTATCCTA GTTATAACGA ATTAATCCTC TTAAAGTTTG TCAATGATGT CCTTGTCGAA 
AAACTCCATG GCAAAAAAGT CCATGGCCAC CCTAGTCATT CCTCCATATT TCAAGTCACC 
AACCTGTCTC TTGGTATGAC TCTGTTTGTC TGCAAGCTAA ACTGTAGCAA CTCTCAAAAG 
ACACAACCAG TGCCAGTGTG TGGAGTGGAG ATCTCAGTCG GTGTTGCTCC AGAGTCACCT 
CAAAGCATAT CATGTGTCCA GAAAGGAGAG CATGGAACTG TGGCCTGCTC CTGGAACTCT 
GGGAAAGTTA CTTATCTGAA AACCTATTAC ACTTTACAGT TAACTGGACC AAACAATGTG 
ACCTATCAGA ACCAGTGTTA TTCTGACAAT CGTCAGAATT GTAGTCGTCT GGATCTTGGG 
ATCAATCTGA CCCCTGATTT AGCCGAATCC AGGTTCATAG TCCATGTTAC TGCCACCAAT 
GATCTTGGAA ATTCTTCTTC ACTTCTGCAT ACATTCACAT TCTTGGACAT AGTGAGGCCT 
CTTCCTCCGT GGGACATCAG AATCAACTTT CTAAATGCTT CTGGGAGCAG ATGTACACTG 
CAGTGGGAAG ATGAGGGGCA AGTGGTACTC AATCGACTCA GATACCAGCC TCTTAACAGC 
ACATCCTGGA ATATGGTTAA TGCTACAAAT GCCAAAGGAA GATATGACCT GCAAGATCTG 
AGACCATTCA CAGAATATGA ATTTCAGATC TCCTCTAAGC TACATCTCTC TCAGGGAAGT 
TGGAGTAATT GGAGTGAGTC ACTGAGAACT CGAACACCAG AGGAAGAGCC TGTTGGGACA 
TTAGACATCT GGTACATGAA ACAAGATATT GACTGTGACA GACAACAGAT CTCTCTTTTC 
TGGAAGAGTC TGAATCCATC AGAGGCAAGG GGGAAGATCC TCCACTATCA GGTGACTTTA 
CAGGAGGTGA CGAAGAAAAC TACACTGCAG AATACTACAA GACATACCTT CTGGACCGGG 
GTCATTCCCA GAACTGGGGC TTGGACTGCA TCTGTGTCTG CAGCCAACTC AAAAGGCACT 
TCTGCACCCA CTCACATTAA CATAGTGGAC CTGTGTGGCA CTGGGTTCCT GGCTCCTCAC 
CAGGTCTCTG CAAAGTCAGG GAACATGGAC AACATCATGG TGACCTGGCA GCCTCCTGGG 
AAAGCTGACT CTGTGATTCG GGAGTACATA GTGGAATGGA GGGCTCTCCA ACAAGGGAGC 
GTCATGAACC TTACCCCACA CTGGCTGCGA ATTCCCCCCT ACAACATGTC TGCTCTGATT 
TCAGAGAAGA TAAAGCCCTA TATCTGTTAC GAAATCCGGG TACATGCACT GTCAGAGGAC 
CAAGGAGGGT GCAGCTCCAT CCGGGGTGAC TCCAGTCACA AAGTGCCACT GAGTGGCCCT 
CATATCACTG CTATCACAGA GAAAAAGGAA AGCCTTTTTA TTTCCTGGAC CCACATCCCT 
TTCCTGGAGC AAAGGGGCTG CATCCTCCAT TACAGAATAT ATTGGAAGGA GCGACACTCT 
ACTGCGCAAC CCGAGCTCTG TGAAATTCAG TACAGACACT CTCAAAACTC ACATCCAATA 
AGCAGCCTAC AGCCCAAGGT GACATATGTC CTATGGATGA CAGCTGTGAC AGCTGCTGGT 
GAAAGTCCCC AAGGAAATGA AAGGGAATTT TGTCCACAGG GTAAGAAAAT AAAGACAGCC 
AGTGAGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447912.1
CDS	486..2417
Translation	MAHTVRGCSLALIFLFMWLLIKANIDVCKLGTVTVQPAPVIPLGSTANISCSLNPKQGCSHYPSYNELILLKFVNDVLVEKLHGKKVHGHPSHSSIFQVTNLSLGMTLFVCKLNCSNSQKTQPVPVCGVEISVGVAPESPQSISCVQKGEHGTVACSWNSGKVTYLKTYYTLQLTGPNNVTYQNQCYSDNRQNCSRLDLGINLTPDLAESRFIVHVTATNDLGNSSSLLHTFTFLDIVRPLPPWDIRINFLNASGSRCTLQWEDEGQVVLNRLRYQPLNSTSWNMVNATNAKGRYDLQDLRPFTEYEFQISSKLHLSQGSWSNWSESLRTRTPEEEPVGTLDIWYMKQDIDCDRQQISLFWKSLNPSEARGKILHYQVTLQEVTKKTTLQNTTRHTFWTGVIPRTGAWTASVSAANSKGTSAPTHINIVDLCGTGFLAPHQVSAKSGNMDNIMVTWQPPGKADSVIREYIVEWRALQQGSVMNLTPHWLRIPPYNMSALISEKIKPYICYEIRVHALSEDQGGCSSIRGDSSHKVPLSGPHITAITEKKESLFISWTHIPFLEQRGCILHYRIYWKERHSTAQPELCEIQYRHSQNSHPISSLQPKVTYVLWMTAVTAAGESPQGNEREFCPQGKKIKTASEF

Target ORF information:

RefSeq Version	XM_017592423.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus interleukin 12 receptor subunit beta 2 (Il12rb2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592423.1

ORF Insert Sequence:

ATGGCACACA CTGTTAGAGG ATGCTCATTG GCACTTATTT TTTTGTTCAT GTGGCTGTTG 
ATTAAAGCAA ATATAGATGT GTGCAAGCTT GGCACCGTGA CTGTCCAGCC TGCCCCTGTG 
ATTCCACTTG GGTCAACTGC CAATATTTCC TGCTCTTTGA ATCCCAAACA AGGCTGTTCG 
CATTATCCTA GTTATAACGA ATTAATCCTC TTAAAGTTTG TCAATGATGT CCTTGTCGAA 
AAACTCCATG GCAAAAAAGT CCATGGCCAC CCTAGTCATT CCTCCATATT TCAAGTCACC 
AACCTGTCTC TTGGTATGAC TCTGTTTGTC TGCAAGCTAA ACTGTAGCAA CTCTCAAAAG 
ACACAACCAG TGCCAGTGTG TGGAGTGGAG ATCTCAGTCG GTGTTGCTCC AGAGTCACCT 
CAAAGCATAT CATGTGTCCA GAAAGGAGAG CATGGAACTG TGGCCTGCTC CTGGAACTCT 
GGGAAAGTTA CTTATCTGAA AACCTATTAC ACTTTACAGT TAACTGGACC AAACAATGTG 
ACCTATCAGA ACCAGTGTTA TTCTGACAAT CGTCAGAATT GTAGTCGTCT GGATCTTGGG 
ATCAATCTGA CCCCTGATTT AGCCGAATCC AGGTTCATAG TCCATGTTAC TGCCACCAAT 
GATCTTGGAA ATTCTTCTTC ACTTCTGCAT ACATTCACAT TCTTGGACAT AGTGAGGCCT 
CTTCCTCCGT GGGACATCAG AATCAACTTT CTAAATGCTT CTGGGAGCAG ATGTACACTG 
CAGTGGGAAG ATGAGGGGCA AGTGGTACTC AATCGACTCA GATACCAGCC TCTTAACAGC 
ACATCCTGGA ATATGGTTAA TGCTACAAAT GCCAAAGGAA GATATGACCT GCAAGATCTG 
AGACCATTCA CAGAATATGA ATTTCAGATC TCCTCTAAGC TACATCTCTC TCAGGGAAGT 
TGGAGTAATT GGAGTGAGTC ACTGAGAACT CGAACACCAG AGGAAGAGCC TGTTGGGACA 
TTAGACATCT GGTACATGAA ACAAGATATT GACTGTGACA GACAACAGAT CTCTCTTTTC 
TGGAAGAGTC TGAATCCATC AGAGGCAAGG GGGAAGATCC TCCACTATCA GGTGACTTTA 
CAGGAGGTGA CGAAGAAAAC TACACTGCAG AATACTACAA GACATACCTT CTGGACCGGG 
GTCATTCCCA GAACTGGGGC TTGGACTGCA TCTGTGTCTG CAGCCAACTC AAAAGGCACT 
TCTGCACCCA CTCACATTAA CATAGTGGAC CTGTGTGGCA CTGGGTTCCT GGCTCCTCAC 
CAGGTCTCTG CAAAGTCAGG GAACATGGAC AACATCATGG TGACCTGGCA GCCTCCTGGG 
AAAGCTGACT CTGTGATTCG GGAGTACATA GTGGAATGGA GGGCTCTCCA ACAAGGGAGC 
GTCATGAACC TTACCCCACA CTGGCTGCGA ATTCCCCCCT ACAACATGTC TGCTCTGATT 
TCAGAGAAGA TAAAGCCCTA TATCTGTTAC GAAATCCGGG TACATGCACT GTCAGAGGAC 
CAAGGAGGGT GCAGCTCCAT CCGGGGTGAC TCCAGTCACA AAGTGCCACT GAGTGGCCCT 
CATATCACTG CTATCACAGA GAAAAAGGAA AGCCTTTTTA TTTCCTGGAC CCACATCCCT 
TTCCTGGAGC AAAGGGGCTG CATCCTCCAT TACAGAATAT ATTGGAAGGA GCGACACTCT 
ACTGCGCAAC CCGAGCTCTG TGAAATTCAG TACAGACACT CTCAAAACTC ACATCCAATA 
AGCAGCCTAC AGCCCAAGGT GACATATGTC CTATGGATGA CAGCTGTGAC AGCTGCTGGT 
GAAAGTCCCC AAGGAAATGA AAGGGAATTT TGTCCACAGG GTAAGAAAAT AAAGACAGCC 
AGTGAGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NOD2 is a negative regulator of Toll-like receptor 2-mediated T helper type 1 responses.
Nature immunology5(8)800-8(2004 Aug)
Watanabe T,Kitani A,Murray PJ,Strober W

A point mutation in the IL-12R beta 2 gene underlies the IL-12 unresponsiveness of Lps-defective C57BL/10ScCr mice.
Journal of immunology (Baltimore, Md. : 1950)167(4)2106-11(2001 Aug)
Poltorak A,Merlin T,Nielsen PJ,Sandra O,Smirnova I,Schupp I,Boehm T,Galanos C,Freudenberg MA

Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.
Immunity13(5)715-25(2000 Nov)
Oppmann B,Lesley R,Blom B,Timans JC,Xu Y,Hunte B,Vega F,Yu N,Wang J,Singh K,Zonin F,Vaisberg E,Churakova T,Liu M,Gorman D,Wagner J,Zurawski S,Liu Y,Abrams JS,Moore KW,Rennick D,de Waal-Malefyt R,Hannum C,Bazan JF,Kastelein RA

Role of beta1 and beta2 subunits of the interleukin-12 receptor in determining T helper 1/T helper 2 responses in vivo in the rat.
Immunology99(1)109-12(2000 Jan)
Gillespie KM,Szeto CC,Betin VM,Mathieson PW

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Il12rb2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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