Ehhadh cDNA ORF clone, Rattus norvegicus(Norway rat)

The isomerase and hydratase reaction mechanism of the crotonase active site of the multifunctional enzyme (type-1), as deduced from structures of complexes with 3S-hydroxy-acyl-CoA.
Kasaragod P, Schmitz W, Hiltunen JK, Wierenga RK
The FEBS journal280(13)3160-75(2013 Jul)

Crystal structure of liganded rat peroxisomal multifunctional enzyme type 1: a flexible molecule with two interconnected active sites.
Kasaragod P, Venkatesan R, Kiema TR, Hiltunen JK, Wierenga RK
The Journal of biological chemistry285(31)24089-98(2010 Jul)

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Ghosh D, Berg JM
Journal of the American Chemical Society132(11)3973-9(2010 Mar)

Regulation of cellular metabolism by protein lysine acetylation.
Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL
Science (New York, N.Y.)327(5968)1000-4(2010 Feb)

Beta-oxidation in hepatocyte cultures from mice with peroxisomal gene knockouts.
Dirkx R, Meyhi E, Asselberghs S, Reddy J, Baes M, Van Veldhoven PP
Biochemical and biophysical research communications357(3)718-23(2007 Jun)

Data from crystallographic binding studies suggest that a catalytic water molecule, bound between two catalytic glutamates (Glu103/Glu123), plays important role in enoyl-CoA isomerase and enoyl-CoA hydratase reaction mechanisms of MFE1.
Title: The isomerase and hydratase reaction mechanism of the crotonase active site of the multifunctional enzyme (type-1), as deduced from structures of complexes with 3S-hydroxy-acyl-CoA.

liganded rat peroxisomal multifunctional enzyme type 1 is a flexible molecule with two interconnected active sites [rpMFE1]
Title: Crystal structure of liganded rat peroxisomal multifunctional enzyme type 1: a flexible molecule with two interconnected active sites.

N-terminal helix of multifunctional enzyme type 1-DH (residues 260-722 and referred to as MFE1-DH) corresponds to the alphaFOM linker helix, located between its hydratase and dehydrogenase part [multifunctional enzyme type 1 ]
Title: Structural studies of MFE-1: the 1.9 A crystal structure of the dehydrogenase part of rat peroxisomal MFE-1.

C-terminal domain E in perMFE-1 serves three functions: participation in folding of N-terminus into a functionally competent H1/I fold, stabilization of the dehydrogenation domains, targeting of the perMFE-1 to peroxisomes
Title: Organization of the multifunctional enzyme type 1: interaction between N- and C-terminal domains is required for the hydratase-1/isomerase activity.