Mlxipl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mlxipl
Entrez Gene ID	171078
Full Name	MLX interacting protein-like
Synonyms	ChREBP,WS-bHLH,Wbscr14,bHLHd14
General protein information	Preferred Names MLX interacting protein-like Names carbohydrate-responsive element-binding protein MLX interactor WS basic-helix-loop-helix leucine zipper protein Williams-Beuren syndrome chromosomal region 14 protein Williams-Beuren syndrome chromosome region 14 homolog class D basic helix-loop-helix protein 14
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q12
Summary	binds the carbohydrate responsive element and regulates transcription of 1-type pyruvate kinase gene (1-PK); DNA binding activity is inhibited by AMP-activated protein kinase (AMPK) phosphorylation of Ser596 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133552.1	NP_598236.1	carbohydrate-responsive element-binding protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MLXIPL	XP_003318716.1
	LOC720093	XP_001115131.2
Canis lupus familiaris (dog)	MLXIPL	XP_005621026.1
Mus musculus (house mouse)	Mlxipl	NP_067430.2
Homo sapiens (human)	MLXIPL	NP_116569.1
Bos taurus (cattle)	MLXIPL	NP_001192337.1
Rattus norvegicus (Norway rat)	Mlxipl	NP_598236.1
Danio rerio (zebrafish)	LOC101882187	XP_005171790.1

Gene Ontology
Bibliography

Related articles in PubMed

Salvianolic acid B protects against chronic alcoholic liver injury via SIRT1-mediated inhibition of CRP and ChREBP in rats.
Zhang N, Hu Y, Ding C, Zeng W, Shan W, Fan H, Zhao Y, Shi X, Gao L, Xu T, Wang R, Gao D, Yao J
Toxicology letters2671-10(2017 Feb)

Histone modifications in FASN modulated by sterol regulatory element-binding protein 1c and carbohydrate responsive-element binding protein under insulin stimulation are related to NAFLD.
Du X, Cai C, Yao J, Zhou Y, Yu H, Shen W
Biochemical and biophysical research communications483(1)409-417(2017 01)

Islet ChREBP-? is increased in diabetes and controls ChREBP-? and glucose-induced gene expression via a negative feedback loop.
Jing G, Chen J, Xu G, Shalev A
Molecular metabolism5(12)1208-1215(2016 Dec)

Integrative Genomics Outlines a Biphasic Glucose Response and a ChREBP-ROR? Axis Regulating Proliferation in ? Cells.
Schmidt SF, Madsen JG, Frafjord K?, Poulsen Ll, Sal? S, Boergesen M, Loft A, Larsen BD, Madsen MS, Holst JJ, Maechler P, Dalgaard LT, Mandrup S
Cell reports16(9)2359-72(2016 08)

New insights into salvianolic acid A action: Regulation of the TXNIP/NLRP3 and TXNIP/ChREBP pathways ameliorates HFD-induced NAFLD in rats.
Ding C, Zhao Y, Shi X, Zhang N, Zu G, Li Z, Zhou J, Gao D, Lv L, Tian X, Yao J
Scientific reports628734(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Salvianolic acid B exerted anti-steatotic and anti-inflammatory effects against alcoholic liver injury by inducing SIRT1-mediated inhibition of CRP and ChREBP expression.
Title: Salvianolic acid B protects against chronic alcoholic liver injury via SIRT1-mediated inhibition of CRP and ChREBP in rats.

The involvement of ChREBP in FASN promoter histone modification.
Title: Histone modifications in FASN modulated by sterol regulatory element-binding protein 1c and carbohydrate responsive-element binding protein under insulin stimulation are related to NAFLD.

Integrative genomics outlines a biphasic glucose response and a ChREBP-RORgamma axis regulating proliferation in pancreatic beta cells.
Title: Integrative Genomics Outlines a Biphasic Glucose Response and a ChREBP-ROR? Axis Regulating Proliferation in ? Cells.

The crystal structure showed that AMP binds directly to the N terminus of ChREBP-alpha2 helix. Our results suggest that AMP inhibits the nuclear localization of ChREBP through an allosteric activation of ChREBP/14-3-3 interactions and not by activation of AMPK.
Title: Metabolite Regulation of Nuclear Localization of Carbohydrate-response Element-binding Protein (ChREBP): ROLE OF AMP AS AN ALLOSTERIC INHIBITOR.

Data suggest expression of ChREBPbeta isoform is up-regulated in pancreatic beta-cells in response to elevated glucose (i.e., hyperglycemic conditions); RNA interference of ChREBPbeta prevents beta-cell proliferation in response to hyperglycemia.
Title: Induction of the ChREBP? Isoform Is Essential for Glucose-Stimulated ?-Cell Proliferation.

The following Mlxipl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mlxipl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00919	NM_133552.1 Latest version!	Rattus norvegicus MLX interacting protein-like (Mlxipl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mlxipl ( NM_133552.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_598236.1 Rattus norvegicus MLX interacting protein-like (Mlxipl), mRNA.

Price & Availability↑

CloneID	ORa00919
Clone ID Related Accession (Same CDS sequence)	NM_133552.1
Accession Version	NM_133552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	carbohydrate-responsive element-binding protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB074517.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB074517.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01906358 [ECO:0000350] ##Evidence-Data-END##

ATGGCGCGCG CGCTGGCGGA TCTATCCGTG AACTTGCAGG TCCCCCGGGT CGTCCCCAGC 
CCGGATTCGG ACTCGGATAC AGACTTGGAG GATCCGAGTC CCCGGCGCAG CGCGGGTGGC 
CTGCACCGAT CACAGGTCAT TCACAGCGGA CACTTCATGG TGTCCTCGCC GCACAGCGAC 
TCGCTGACCC GGCGACGCGA CCAGGAGGGG CCCGTGGGGC TCGCCGACTT CGGGCCGCGC 
AGCATCGATC CGACACTCAC CCGCCTCTTC GAATGCTTGA GCCTGGCTTA CAGCGGCAAG 
CTGGTGTCTC CCAAGTGGAA GAACTTCAAA GGCCTCAAGT TGCTATGCCG GGACAAGATT 
CGGCTGAACA ACGCCATCTG GAGAGCCTGG TACATTCAGT ATGTGCAACG GAGGAAGAGC 
CCAGTATGTG GCTTCGTAAC TCCTCTGCAG GGGTCAGAAG CAGATGAGCA CCGGAAACCT 
GAGGCTGTTG TCTTGGAGGG TAATTACTGG AAGCGGCGCA TCGAGGTGGT GATGCGTGAA 
TACCACAAGT GGAGAATCTA CTACAAGAAG CGGCTCCGTA AGTCCAGCAG GGAAGGGGAT 
TTCCTGGCTC CCAAGCAGGT GGAAGGTGGG TGGCCGCCAC CAGAGAGATG GTGCGAACAG 
CTCTTCTCCA GCGTGGTGCC TGTGCTGCTT GGGGGCTCCG AGGAGGAGCC TGGGGGCCGG 
CAGCTTCTCG ACTTGGACTG CTTCTTGTCC GATATCTCCG ACACACTCTT CACCATGACA 
CAGCCCAGCC CCTCGTCCCT GCAGCTGCCC TCAGAAGACG CTTATGTTGG CAATGCTGAC 
ATGATCCAGC CAGACCTGAC GCCACTGCAG CCCAGCCTGG ATGACTTCAT GGAGATATCA 
GATTTCTTCA CCAATTATCG CCCCCCTCAG ACGCCCACAT CTTCAAACTT CCCAGAGCCC 
CCCAGCTTTG GCCCCATGGC TGACTCCCTC TTCAGCGGTG GGATCCTGGG CCCAGAGATG 
CCATCTCCAG CCTCCGCTTC CTCCTCCTCG GGGATGACCC CTCTCTCAGG GAATACACGT 
CTCCAGGCTC GAAACAGCTG CTCTGGACCC TTGGATCCCA GCACCTTTCC GAGCTCTGAA 
TTCCTTCTTC CTGAAGACCC AAAGACCAAG ATGCCACCTG CTCCTGTACC GACTCCTCTC 
CTTCCGTACC CCGGCCCTGT CAAGGTGCAT GGCTTAGAGC CCTGCACTCC ATCTCCCTTC 
CCCACGATGG CTCCACCTCC TGCTTTGTTG TCAGAAGAGC CTCTCTTCTC TGCAAGGTTC 
CCCTTCACCA CAGTCCCTCC CGCTCCAGGA GTGTCTACCC TTCCTGCACC CACAACCTTT 
GTTCCCACGC CACAGCCTGG CCCTGGCCCT GGCCCTGTCC CTTTCCCCGT AGACCATCTG 
CCCCATGGGT ACCTGGAACC TGTCTTTGGG CCTCACTTCA CGGTGCCTCA GGGTGTGCAG 
CCCAGATGCA AGCCCTGCTC TCCACCTCCT GGGGGACGAA AAGCCAGTCC ACCCACCTTG 
ACCTCTGCCA CTGCCAGCCC CACGGCCACT GCTACTGCCA GAGACAACAA CCCCTGCCTT 
ACACAGCTGC TCAGGGCAGC CAAGCCTGAA CAAGTACTGG AACCTTCCAC TGTGCCCAGC 
ACCCTCCTCC GGCCTCCAGA GTCCCCGGAT GCAGTCCCTG AAATCCCCCG TGTCCGTGCA 
TTTTACCCCC CAATCCCAGC CCCTACACCA CCTAGGCCAC CTCCAGGCCC TGCCACATTG 
GCTCCTCCCA GGTCCCTGGT TGTCCCCAAA GCAGAGAGGC TCTCACCTCC AGCATCCAGC 
GGCAGTGAGC GGCGACCATC AGGGGATCTC AACTCCATTC AACCCCCGGG GGCACTGAGT 
GTCCACCTGT CTCCCCCTCA AACTGTCCTA AGCCGGGGTC GTGTAGACAA CAACAAGATG 
GAGAACCGTC GAATCACACA CATCTCCGCA GAGCAGAAGA GGCGTTTCAA TATTAAGCTA 
GGATTTGACA CTCTGCACGG ACTTGTCAGC ACGCTCAGTG CCCAGCCCAG CCTCAAGGTG 
AGCAAAGCAA CCACGCTTCA GAAGACGGCG GAGTACATCC TGATGCTACA GCAGGAGCGG 
GCGGCCATGC AGGAGGAAGC GCAGCAACTG AGGGATGAAA TAGAGGAGCT CAATGCTGCC 
ATCAACTTGT GCCAGCAGCA GCTACCGGCC ACCGGGGTGC CCATCACACA TCAGCGCTTT 
GACCAGATGC GGGACATGTT TGATGACTAT GTCCGGACCC GCACGCTGCA CAACTGGAAG 
TTCTGGGTAT TCAGCATCCT CATCCGACCT TTATTTGAGT CCTTCAATGG GATGGTGTCT 
ACCGCAAGCT TGCACAGCCT CCGCCAGACC TCACTGGCAT GGCTGGACCA GTACTGTTCC 
CTGCCTGCTC TCCGGCCAAC TGTCCTGAAT TCCCTTCGCC AGCTCAGCAC TTCCACCAGC 
ATCCTGACTG ACCCGAGCCT CGTGCCTGAG CAAGCCACAA GGGCAGTCAC AGAGGGTCCC 
CTGGGCAGAC CATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598236.1
CDS	34..2631
Translation	MARALADLSVNLQVPRVVPSPDSDSDTDLEDPSPRRSAGGLHRSQVIHSGHFMVSSPHSDSLTRRRDQEGPVGLADFGPRSIDPTLTRLFECLSLAYSGKLVSPKWKNFKGLKLLCRDKIRLNNAIWRAWYIQYVQRRKSPVCGFVTPLQGSEADEHRKPEAVVLEGNYWKRRIEVVMREYHKWRIYYKKRLRKSSREGDFLAPKQVEGGWPPPERWCEQLFSSVVPVLLGGSEEEPGGRQLLDLDCFLSDISDTLFTMTQPSPSSLQLPSEDAYVGNADMIQPDLTPLQPSLDDFMEISDFFTNYRPPQTPTSSNFPEPPSFGPMADSLFSGGILGPEMPSPASASSSSGMTPLSGNTRLQARNSCSGPLDPSTFPSSEFLLPEDPKTKMPPAPVPTPLLPYPGPVKVHGLEPCTPSPFPTMAPPPALLSEEPLFSARFPFTTVPPAPGVSTLPAPTTFVPTPQPGPGPGPVPFPVDHLPHGYLEPVFGPHFTVPQGVQPRCKPCSPPPGGRKASPPTLTSATASPTATATARDNNPCLTQLLRAAKPEQVLEPSTVPSTLLRPPESPDAVPEIPRVRAFYPPIPAPTPPRPPPGPATLAPPRSLVVPKAERLSPPASSGSERRPSGDLNSIQPPGALSVHLSPPQTVLSRGRVDNNKMENRRITHISAEQKRRFNIKLGFDTLHGLVSTLSAQPSLKVSKATTLQKTAEYILMLQQERAAMQEEAQQLRDEIEELNAAINLCQQQLPATGVPITHQRFDQMRDMFDDYVRTRTLHNWKFWVFSILIRPLFESFNGMVSTASLHSLRQTSLAWLDQYCSLPALRPTVLNSLRQLSTSTSILTDPSLVPEQATRAVTEGPLGRPL

Target ORF information:

RefSeq Version	NM_133552.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MLX interacting protein-like (Mlxipl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133552.1

ORF Insert Sequence:

ATGGCGCGCG CGCTGGCGGA TCTATCCGTG AACTTGCAGG TCCCCCGGGT CGTCCCCAGC 
CCGGATTCGG ACTCGGATAC AGACTTGGAG GATCCGAGTC CCCGGCGCAG CGCGGGTGGC 
CTGCACCGAT CACAGGTCAT TCACAGCGGA CACTTCATGG TGTCCTCGCC GCACAGCGAC 
TCGCTGACCC GGCGACGCGA CCAGGAGGGG CCCGTGGGGC TCGCCGACTT CGGGCCGCGC 
AGCATCGATC CGACACTCAC CCGCCTCTTC GAATGCTTGA GCCTGGCTTA CAGCGGCAAG 
CTGGTGTCTC CCAAGTGGAA GAACTTCAAA GGCCTCAAGT TGCTATGCCG GGACAAGATT 
CGGCTGAACA ACGCCATCTG GAGAGCCTGG TACATTCAGT ATGTGCAACG GAGGAAGAGC 
CCAGTATGTG GCTTCGTAAC TCCTCTGCAG GGGTCAGAAG CAGATGAGCA CCGGAAACCT 
GAGGCTGTTG TCTTGGAGGG TAATTACTGG AAGCGGCGCA TCGAGGTGGT GATGCGTGAA 
TACCACAAGT GGAGAATCTA CTACAAGAAG CGGCTCCGTA AGTCCAGCAG GGAAGGGGAT 
TTCCTGGCTC CCAAGCAGGT GGAAGGTGGG TGGCCGCCAC CAGAGAGATG GTGCGAACAG 
CTCTTCTCCA GCGTGGTGCC TGTGCTGCTT GGGGGCTCCG AGGAGGAGCC TGGGGGCCGG 
CAGCTTCTCG ACTTGGACTG CTTCTTGTCC GATATCTCCG ACACACTCTT CACCATGACA 
CAGCCCAGCC CCTCGTCCCT GCAGCTGCCC TCAGAAGACG CTTATGTTGG CAATGCTGAC 
ATGATCCAGC CAGACCTGAC GCCACTGCAG CCCAGCCTGG ATGACTTCAT GGAGATATCA 
GATTTCTTCA CCAATTATCG CCCCCCTCAG ACGCCCACAT CTTCAAACTT CCCAGAGCCC 
CCCAGCTTTG GCCCCATGGC TGACTCCCTC TTCAGCGGTG GGATCCTGGG CCCAGAGATG 
CCATCTCCAG CCTCCGCTTC CTCCTCCTCG GGGATGACCC CTCTCTCAGG GAATACACGT 
CTCCAGGCTC GAAACAGCTG CTCTGGACCC TTGGATCCCA GCACCTTTCC GAGCTCTGAA 
TTCCTTCTTC CTGAAGACCC AAAGACCAAG ATGCCACCTG CTCCTGTACC GACTCCTCTC 
CTTCCGTACC CCGGCCCTGT CAAGGTGCAT GGCTTAGAGC CCTGCACTCC ATCTCCCTTC 
CCCACGATGG CTCCACCTCC TGCTTTGTTG TCAGAAGAGC CTCTCTTCTC TGCAAGGTTC 
CCCTTCACCA CAGTCCCTCC CGCTCCAGGA GTGTCTACCC TTCCTGCACC CACAACCTTT 
GTTCCCACGC CACAGCCTGG CCCTGGCCCT GGCCCTGTCC CTTTCCCCGT AGACCATCTG 
CCCCATGGGT ACCTGGAACC TGTCTTTGGG CCTCACTTCA CGGTGCCTCA GGGTGTGCAG 
CCCAGATGCA AGCCCTGCTC TCCACCTCCT GGGGGACGAA AAGCCAGTCC ACCCACCTTG 
ACCTCTGCCA CTGCCAGCCC CACGGCCACT GCTACTGCCA GAGACAACAA CCCCTGCCTT 
ACACAGCTGC TCAGGGCAGC CAAGCCTGAA CAAGTACTGG AACCTTCCAC TGTGCCCAGC 
ACCCTCCTCC GGCCTCCAGA GTCCCCGGAT GCAGTCCCTG AAATCCCCCG TGTCCGTGCA 
TTTTACCCCC CAATCCCAGC CCCTACACCA CCTAGGCCAC CTCCAGGCCC TGCCACATTG 
GCTCCTCCCA GGTCCCTGGT TGTCCCCAAA GCAGAGAGGC TCTCACCTCC AGCATCCAGC 
GGCAGTGAGC GGCGACCATC AGGGGATCTC AACTCCATTC AACCCCCGGG GGCACTGAGT 
GTCCACCTGT CTCCCCCTCA AACTGTCCTA AGCCGGGGTC GTGTAGACAA CAACAAGATG 
GAGAACCGTC GAATCACACA CATCTCCGCA GAGCAGAAGA GGCGTTTCAA TATTAAGCTA 
GGATTTGACA CTCTGCACGG ACTTGTCAGC ACGCTCAGTG CCCAGCCCAG CCTCAAGGTG 
AGCAAAGCAA CCACGCTTCA GAAGACGGCG GAGTACATCC TGATGCTACA GCAGGAGCGG 
GCGGCCATGC AGGAGGAAGC GCAGCAACTG AGGGATGAAA TAGAGGAGCT CAATGCTGCC 
ATCAACTTGT GCCAGCAGCA GCTACCGGCC ACCGGGGTGC CCATCACACA TCAGCGCTTT 
GACCAGATGC GGGACATGTT TGATGACTAT GTCCGGACCC GCACGCTGCA CAACTGGAAG 
TTCTGGGTAT TCAGCATCCT CATCCGACCT TTATTTGAGT CCTTCAATGG GATGGTGTCT 
ACCGCAAGCT TGCACAGCCT CCGCCAGACC TCACTGGCAT GGCTGGACCA GTACTGTTCC 
CTGCCTGCTC TCCGGCCAAC TGTCCTGAAT TCCCTTCGCC AGCTCAGCAC TTCCACCAGC 
ATCCTGACTG ACCCGAGCCT CGTGCCTGAG CAAGCCACAA GGGCAGTCAC AGAGGGTCCC 
CTGGGCAGAC CATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Salvianolic acid B protects against chronic alcoholic liver injury via SIRT1-mediated inhibition of CRP and ChREBP in rats.
Toxicology letters2671-10(2017 Feb)
Zhang N,Hu Y,Ding C,Zeng W,Shan W,Fan H,Zhao Y,Shi X,Gao L,Xu T,Wang R,Gao D,Yao J

Histone modifications in FASN modulated by sterol regulatory element-binding protein 1c and carbohydrate responsive-element binding protein under insulin stimulation are related to NAFLD.
Biochemical and biophysical research communications483(1)409-417(2017 01)
Du X,Cai C,Yao J,Zhou Y,Yu H,Shen W

Islet ChREBP-? is increased in diabetes and controls ChREBP-? and glucose-induced gene expression via a negative feedback loop.
Molecular metabolism5(12)1208-1215(2016 Dec)
Jing G,Chen J,Xu G,Shalev A

Integrative Genomics Outlines a Biphasic Glucose Response and a ChREBP-ROR? Axis Regulating Proliferation in ? Cells.
Cell reports16(9)2359-72(2016 08)
Schmidt SF,Madsen JG,Frafjord K?,Poulsen Ll,Sal? S,Boergesen M,Loft A,Larsen BD,Madsen MS,Holst JJ,Maechler P,Dalgaard LT,Mandrup S

New insights into salvianolic acid A action: Regulation of the TXNIP/NLRP3 and TXNIP/ChREBP pathways ameliorates HFD-induced NAFLD in rats.
Scientific reports628734(2016 Jun)
Ding C,Zhao Y,Shi X,Zhang N,Zu G,Li Z,Zhou J,Gao D,Lv L,Tian X,Yao J

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Mlxipl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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