Cry2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cry2
Entrez Gene ID	170917
Full Name	cryptochrome circadian regulator 2
General protein information	Preferred Names cryptochrome circadian regulator 2 Names cryptochrome-2 cryptochrome 2 (photolyase-like) cryptochrome circadian clock 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q24
Summary	This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133405.2	NP_596896.2	cryptochrome-2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC509058	XP_002693610.3
Homo sapiens (human)	CRY2	NP_066940.2
Pan troglodytes (chimpanzee)	CRY2	XP_001160595.2
Danio rerio (zebrafish)	cry3	NP_571861.2
Canis lupus familiaris (dog)	CRY2	XP_540761.2
Mus musculus (house mouse)	Cry2	NP_034093.1
Rattus norvegicus (Norway rat)	Cry2	NP_596896.1
Gallus gallus (chicken)	CRY2	NP_989575.1
Xenopus tropicalis (tropical clawed frog)	cry2	XP_002934138.2
	CRY2	XP_001113162.1

Gene Ontology
Bibliography

Related articles in PubMed

Circadian repressors CRY1 and CRY2 broadly interact with nuclear receptors and modulate transcriptional activity.
Kriebs A, Jordan SD, Soto E, Henriksson E, Sandate CR, Vaughan ME, Chan AB, Duglan D, Papp SJ, Huber AL, Afetian ME, Yu RT, Zhao X, Downes M, Evans RM, Lamia KA
Proceedings of the National Academy of Sciences of the United States of America114(33)8776-8781(2017 Aug)

CRY1/2 Selectively Repress PPAR? and Limit Exercise Capacity.
Jordan SD, Kriebs A, Vaughan M, Duglan D, Fan W, Henriksson E, Huber AL, Papp SJ, Nguyen M, Afetian M, Downes M, Yu RT, Kralli A, Evans RM, Lamia KA
Cell metabolism26(1)243-255.e6(2017 Jul)

Differential role of melatonin in restoration of age-induced alterations in daily rhythms of expression of various clock genes in suprachiasmatic nucleus of male Wistar rats.
Mattam U, Jagota A
Biogerontology15(3)257-68(2014 Jun)

A novel protein, CHRONO, functions as a core component of the mammalian circadian clock.
Goriki A, Hatanaka F, Myung J, Kim JK, Yoritaka T, Tanoue S, Abe T, Kiyonari H, Fujimoto K, Kato Y, Todo T, Matsubara A, Forger D, Takumi T
PLoS biology12(4)e1001839(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that there is a 24-h rhythm in the expression of Per2 and Cry2 in males and females.
Title: Gender associated circadian oscillations of the clock genes in rat choroid plexus.

We investigated the effect of aging on daily rhythms of various clock genes such as rPer1, rPer2, rCry1, rCry2 and rBmal1 in the SCN of male Wistar rats.
Title: Differential role of melatonin in restoration of age-induced alterations in daily rhythms of expression of various clock genes in suprachiasmatic nucleus of male Wistar rats.

N-nitroso-N-methylurea disrupted the expression of Cry2 in mammary glands of Fischer 344 rats
Title: Methylselenocysteine resets the rhythmic expression of circadian and growth-regulatory genes disrupted by nitrosomethylurea in vivo.

cry2 may function in circadian photoreception in the rat brain
Title: Cloning and expression of cryptochrome2 cDNA in the rat.

The following Cry2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cry2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13704	NM_133405.2 Latest version!	Rattus norvegicus cryptochrome circadian regulator 2 (Cry2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cry2 ( NM_133405.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_596896.2 Rattus norvegicus cryptochrome circadian regulator 2 (Cry2), mRNA.

Price & Availability↑

CloneID	ORa13704
Clone ID Related Accession (Same CDS sequence)	NM_133405.2
Accession Version	NM_133405.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	cryptochrome-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166416.1. On or before Feb 5, 2014 this sequence version replaced XM_006234547.1, NM_133405.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC166416.1, FQ211347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CTGCTGTGGT GGCAGCGACG GTCCCCGCGC AGTCGATGGG CGCGGACGGC 
GCGTCCTCCG TGCACTGGTT CCGCAAAGGA CTACGGCTCC ACGACAACCC CGCGCTGTTA 
GCTGCCGTGC GCGGGGCGCG CTGTGTGCGC TGCGTCTACA TCCTCGACCC GTGGTTCGCG 
GCCTCCTCGT CAGTGGGCAT CAACCGATGG AGGTTCCTAC TGCAGTCTCT AGAAGATCTG 
GACACAAGCT TAAGAAAGCT GAATTCCCGT CTGTTTGTAG TCCGGGGTCA GCCAGCTGAT 
GTGTTCCCAA GGCTTTTCAA GGAATGGGGG GTGACCCGCT TGACCTTTGA ATATGACTCC 
GAACCCTTTG GGAAAGAACG GGATGCAGCC ATTATGAAGA TGGCCAAGGA GGCGGGTGTG 
GAGGTGGTGA CTGAGAACTC TCACACCCTT TATGACTTAG ACAGAATCAT CGAACTGAAT 
GGGCAGAAAC CACCCCTTAC CTACAAGCGC TTTCAGGCTC TCATCAGCCG TATGGAGCTG 
CCCAAGAAGC CAGTGGGGGC TGTGAGCAGC CAGCATATGG AGAACTGCAG AGCTGAGATC 
CAGGAGAACC ATGATGACAC CTATGGCGTG CCTTCCTTAG AGGAACTGGG ATTCCCCACA 
GAAGGACTTG GCCCAGCTGT TTGGCAAGGA GGAGAGACAG AAGCTCTGGC CCGCCTGGAT 
AAGCACTTGG AACGGAAGGC CTGGGTTGCC AACTATGAGA GACCTCGGAT GAATGCCAAT 
TCCTTGCTGG CCAGCCCCAC AGGCCTCAGC CCCTACCTGC GCTTTGGCTG CCTCTCCTGC 
CGCCTCTTCT ACTACCGCCT GTGGGACTTG TACAGAAAGG TGAAGAGGAA CAGCACACCC 
CCCCTCTCCT TATTTGGACA ACTCCTATGG CGAGAATTCT TCTATACAGC GGCCACCAAC 
AACCCCAGGT TTGACCGAAT GGAGGGGAAC CCCATCTGCA TCCAGATCCC CTGGGACCGC 
AACCCCGAAG CCCTGGCCAA GTGGGCCGAG GGCAAGACAG GCTTCCCTTG GATTGACGCC 
ATCATGACCC AACTGAGGCA GGAGGGCTGG ATCCACCACC TGGCCCGGCA CGCTGTGGCC 
TGCTTCCTCA CCCGAGGGGA CCTCTGGGTC AGCTGGGAGA GCGGGGTCCG GGTATTTGAT 
GAGTTGCTCC TGGATGCAGA TTTCAGCGTG AATGCAGGCA GCTGGATGTG GCTGTCCTGC 
AGTGCTTTCT TCCAACAGTT CTTCCACTGC TACTGCCCTG TGGGCTTTGG CCGACGCACG 
GACCCCAGTG GGGACTACAT CCGGCGATAC CTGCCCAAAC TGAAAGGCTT CCCCTCTCGA 
TATATCTATG AGCCCTGGAA TGCTCCCGAG TCGGTTCAGA AGGCCGCTAA GTGCATCATT 
GGCGTGGACT ACCCACGGCC CATCGTCAAC CACGCAGAGA CTAGTCGGCT CAACATTGAG 
CGGATGAAGC AGATCTACCA ACAGCTGTCA CGATACCGGG GGCTCTGTCT GTTGGCATCT 
GTCCCTTCCT GTGTAGAAGA CCTCAGTCAC CCTGTGGCAG AGCCTGGTTC TAGCCAGGCT 
GGGAGCATCA GCAACACAGG CCCCAGACCA CTGTCCAGTG GCCCAGCCTC CCCCAAACGC 
AAGCTGGAAG CAGCTGAGGA ACCTCCAGGT GAAGAACTGA GCAAGCGGGC TAGAGTGACA 
GTGACTCAGA TGCCTGCCCA GGAGCCACCA AGCAAGGACT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596896.2
CDS	3..1787
Translation	MAAAAVVAATVPAQSMGADGASSVHWFRKGLRLHDNPALLAAVRGARCVRCVYILDPWFAASSSVGINRWRFLLQSLEDLDTSLRKLNSRLFVVRGQPADVFPRLFKEWGVTRLTFEYDSEPFGKERDAAIMKMAKEAGVEVVTENSHTLYDLDRIIELNGQKPPLTYKRFQALISRMELPKKPVGAVSSQHMENCRAEIQENHDDTYGVPSLEELGFPTEGLGPAVWQGGETEALARLDKHLERKAWVANYERPRMNANSLLASPTGLSPYLRFGCLSCRLFYYRLWDLYRKVKRNSTPPLSLFGQLLWREFFYTAATNNPRFDRMEGNPICIQIPWDRNPEALAKWAEGKTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWVSWESGVRVFDELLLDADFSVNAGSWMWLSCSAFFQQFFHCYCPVGFGRRTDPSGDYIRRYLPKLKGFPSRYIYEPWNAPESVQKAAKCIIGVDYPRPIVNHAETSRLNIERMKQIYQQLSRYRGLCLLASVPSCVEDLSHPVAEPGSSQAGSISNTGPRPLSSGPASPKRKLEAAEEPPGEELSKRARVTVTQMPAQEPPSKDS

Target ORF information:

RefSeq Version	NM_133405.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cryptochrome circadian regulator 2 (Cry2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133405.2

ORF Insert Sequence:

ATGGCGGCGG CTGCTGTGGT GGCAGCGACG GTCCCCGCGC AGTCGATGGG CGCGGACGGC 
GCGTCCTCCG TGCACTGGTT CCGCAAAGGA CTACGGCTCC ACGACAACCC CGCGCTGTTA 
GCTGCCGTGC GCGGGGCGCG CTGTGTGCGC TGCGTCTACA TCCTCGACCC GTGGTTCGCG 
GCCTCCTCGT CAGTGGGCAT CAACCGATGG AGGTTCCTAC TGCAGTCTCT AGAAGATCTG 
GACACAAGCT TAAGAAAGCT GAATTCCCGT CTGTTTGTAG TCCGGGGTCA GCCAGCTGAT 
GTGTTCCCAA GGCTTTTCAA GGAATGGGGG GTGACCCGCT TGACCTTTGA ATATGACTCC 
GAACCCTTTG GGAAAGAACG GGATGCAGCC ATTATGAAGA TGGCCAAGGA GGCGGGTGTG 
GAGGTGGTGA CTGAGAACTC TCACACCCTT TATGACTTAG ACAGAATCAT CGAACTGAAT 
GGGCAGAAAC CACCCCTTAC CTACAAGCGC TTTCAGGCTC TCATCAGCCG TATGGAGCTG 
CCCAAGAAGC CAGTGGGGGC TGTGAGCAGC CAGCATATGG AGAACTGCAG AGCTGAGATC 
CAGGAGAACC ATGATGACAC CTATGGCGTG CCTTCCTTAG AGGAACTGGG ATTCCCCACA 
GAAGGACTTG GCCCAGCTGT TTGGCAAGGA GGAGAGACAG AAGCTCTGGC CCGCCTGGAT 
AAGCACTTGG AACGGAAGGC CTGGGTTGCC AACTATGAGA GACCTCGGAT GAATGCCAAT 
TCCTTGCTGG CCAGCCCCAC AGGCCTCAGC CCCTACCTGC GCTTTGGCTG CCTCTCCTGC 
CGCCTCTTCT ACTACCGCCT GTGGGACTTG TACAGAAAGG TGAAGAGGAA CAGCACACCC 
CCCCTCTCCT TATTTGGACA ACTCCTATGG CGAGAATTCT TCTATACAGC GGCCACCAAC 
AACCCCAGGT TTGACCGAAT GGAGGGGAAC CCCATCTGCA TCCAGATCCC CTGGGACCGC 
AACCCCGAAG CCCTGGCCAA GTGGGCCGAG GGCAAGACAG GCTTCCCTTG GATTGACGCC 
ATCATGACCC AACTGAGGCA GGAGGGCTGG ATCCACCACC TGGCCCGGCA CGCTGTGGCC 
TGCTTCCTCA CCCGAGGGGA CCTCTGGGTC AGCTGGGAGA GCGGGGTCCG GGTATTTGAT 
GAGTTGCTCC TGGATGCAGA TTTCAGCGTG AATGCAGGCA GCTGGATGTG GCTGTCCTGC 
AGTGCTTTCT TCCAACAGTT CTTCCACTGC TACTGCCCTG TGGGCTTTGG CCGACGCACG 
GACCCCAGTG GGGACTACAT CCGGCGATAC CTGCCCAAAC TGAAAGGCTT CCCCTCTCGA 
TATATCTATG AGCCCTGGAA TGCTCCCGAG TCGGTTCAGA AGGCCGCTAA GTGCATCATT 
GGCGTGGACT ACCCACGGCC CATCGTCAAC CACGCAGAGA CTAGTCGGCT CAACATTGAG 
CGGATGAAGC AGATCTACCA ACAGCTGTCA CGATACCGGG GGCTCTGTCT GTTGGCATCT 
GTCCCTTCCT GTGTAGAAGA CCTCAGTCAC CCTGTGGCAG AGCCTGGTTC TAGCCAGGCT 
GGGAGCATCA GCAACACAGG CCCCAGACCA CTGTCCAGTG GCCCAGCCTC CCCCAAACGC 
AAGCTGGAAG CAGCTGAGGA ACCTCCAGGT GAAGAACTGA GCAAGCGGGC TAGAGTGACA 
GTGACTCAGA TGCCTGCCCA GGAGCCACCA AGCAAGGACT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Circadian repressors CRY1 and CRY2 broadly interact with nuclear receptors and modulate transcriptional activity.
Proceedings of the National Academy of Sciences of the United States of America114(33)8776-8781(2017 Aug)
Kriebs A,Jordan SD,Soto E,Henriksson E,Sandate CR,Vaughan ME,Chan AB,Duglan D,Papp SJ,Huber AL,Afetian ME,Yu RT,Zhao X,Downes M,Evans RM,Lamia KA

CRY1/2 Selectively Repress PPAR? and Limit Exercise Capacity.
Cell metabolism26(1)243-255.e6(2017 Jul)
Jordan SD,Kriebs A,Vaughan M,Duglan D,Fan W,Henriksson E,Huber AL,Papp SJ,Nguyen M,Afetian M,Downes M,Yu RT,Kralli A,Evans RM,Lamia KA

Differential role of melatonin in restoration of age-induced alterations in daily rhythms of expression of various clock genes in suprachiasmatic nucleus of male Wistar rats.
Biogerontology15(3)257-68(2014 Jun)
Mattam U,Jagota A

A novel protein, CHRONO, functions as a core component of the mammalian circadian clock.
PLoS biology12(4)e1001839(2014 Apr)
Goriki A,Hatanaka F,Myung J,Kim JK,Yoritaka T,Tanoue S,Abe T,Kiyonari H,Fujimoto K,Kato Y,Todo T,Matsubara A,Forger D,Takumi T

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Cry2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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