Lonp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lonp1
Entrez Gene ID	170916
Full Name	lon peptidase 1, mitochondrial
Synonyms	Lon,Prss15
General protein information	Preferred Names lon peptidase 1, mitochondrial Names lon protease homolog, mitochondrial LONP lon protease-like protein mitochondrial ATP-dependent protease Lon protease, serine, 15 serine protease 15
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q12
Summary	mitochondrial ATP-dependent protease that displays increased expression under conditions of brain ischemia, hypoxia, and ER stress [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133404.1	NP_596895.1	lon protease homolog, mitochondrial precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LONP1	XP_001143873.1
Canis lupus familiaris (dog)	LONP1	XP_854391.2
Mus musculus (house mouse)	Lonp1	NP_083058.2
Rattus norvegicus (Norway rat)	Lonp1	NP_596895.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010451	XP_311497.4
Caenorhabditis elegans (roundworm)	C34B2.6	NP_492796.1
Homo sapiens (human)	LONP1	NP_004784.2
Bos taurus (cattle)	LONP1	NP_001015569.2
Danio rerio (zebrafish)	lonp1	XP_691712.4
Saccharomyces cerevisiae (baker's yeast)	PIM1	NP_009531.1
Kluyveromyces lactis	KLLA0E10407g	XP_454420.1
Magnaporthe oryzae (rice blast fungus)	MGG_11141	XP_003714792.1
Xenopus tropicalis (tropical clawed frog)	LOC100492505	XP_002938599.1
	LONP1	XP_001088663.2
Gallus gallus (chicken)	LONP1	XP_001232112.2
Oryza sativa (rice)	Os07g0689300	NP_001175344.1
Schizosaccharomyces pombe (fission yeast)	lon1	NP_593035.1
Drosophila melanogaster (fruit fly)	Lon	NP_649133.1
Neurospora crassa	NCU05261	XP_961826.1
Arabidopsis thaliana (thale cress)	LON1	NP_568490.3

Gene Ontology
Bibliography

Related articles in PubMed

Oxygen-sensitive mitochondrial accumulation of cystathionine ?-synthase mediated by Lon protease.
Teng H, Wu B, Zhao K, Yang G, Wu L, Wang R
Proceedings of the National Academy of Sciences of the United States of America110(31)12679-84(2013 Jul)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Susceptibility of mitochondrial superoxide dismutase to aluminium induced oxidative damage.
Kumar V, Bal A, Gill KD
Toxicology255(3)117-23(2009 Jan)

Inactivation of brain mitochondrial Lon protease by peroxynitrite precedes electron transport chain dysfunction.
Stanyer L, Jorgensen W, Hori O, Clark JB, Heales SJ
Neurochemistry international53(3-4)95-101(2008 Sep)

Thermodynamic characterization of specific interactions between the human Lon protease and G-quartet DNA.
Chen SH, Suzuki CK, Wu SH
Nucleic acids research36(4)1273-87(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lon protease, a major degradation enzyme in mitochondrial matrix, recognized and degraded mitochondrial CBS by specifically targeting at the oxygenated heme group of CBS proteins.
Title: Oxygen-sensitive mitochondrial accumulation of cystathionine ?-synthase mediated by Lon protease.

Taken together these findings suggest that Lon protease may be particularly vulnerable to inactivation in conditions associated with GSH depletion and elevated oxidative stress.
Title: Inactivation of brain mitochondrial Lon protease by peroxynitrite precedes electron transport chain dysfunction.

Identification of a newly identified peroxisome-specific isoform of Lon protease, an ATP-dependent protease with chaperone-like activity
Title: Proteomic analysis of rat liver peroxisome: presence of peroxisome-specific isozyme of Lon protease.

changes in nuclear gene expression (Lon) triggered by ER stress had the potential to impact important mitochondrial processes such as assembly and/or degradation of cytochrome c oxidase
Title: Transmission of cell stress from endoplasmic reticulum to mitochondria: enhanced expression of Lon protease.

The following Lonp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lonp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13034	NM_133404.1 Latest version!	Rattus norvegicus lon peptidase 1, mitochondrial (Lonp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lonp1 ( NM_133404.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_596895.1 Rattus norvegicus lon peptidase 1, mitochondrial (Lonp1), mRNA.

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CloneID	ORa13034
Clone ID Related Accession (Same CDS sequence)	NM_133404.1
Accession Version	NM_133404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	lon protease homolog, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB064323.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB064323.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCAA GCACAGGTTA CGTGCGGCTG TGGGCTGCTG CGCGGTGCTG GGTTCTGCGG 
CGTCCGCTCT TGGCTGTCAC CGGAGGCCGA GTCCCGAGTG CGTCCGGGTC CTGGCTGCGC 
CGAGGATGTC GGGTCTGCGA CACGTCAACT CCCTGGGGCG GCCGCGTTCC TATGGGCGGG 
GGCCAGTGGC GGGGGCTGTG GGACGCGGGG AGCCGCGGTG GCAGCGACGA GACCTCCGAG 
GGCGGCGTGG AGGACGGGGC CACGGCGAGC AGCGGGGAAG GCCCGGTCGT CACGGCCCTC 
GCGCCTATGA CCGTCCCGGA TGTGTTTCCT CACCTGCCGC TCATCGCCAT CTCCCGCAAC 
CCGGTGTTCC CGCGCTTTAT CAAGATCGTG GAGGTTAAAA ATAAGAAGTT GGTTGAGCTG 
CTGAGAAGGA AAGTCCGCCT GGCACAGCCG TATGTTGGCG TCTTCTTAAA GAGAGATGAT 
AACAATGAGT CAGACGTGGT GGAGAGCCTG GATGAGATCT ACCATACGGG AACGTTCGCT 
CAGATTCATG AGATGCAGGA CCTCGGAGAC AAGTTGCGCA TGATCGTTAC AGGCCACAGA 
AGGATTCACA TCAGCCGGCA GCTGGAGGTG GAACCAGAGG GGCTGGAGCC AGAGGCAGAG 
AACAAACAAA AGTCCAGGAG GAAGCTGAAG CGAGGCAAGA AAGAGGTCGG GGATGAGCTG 
GGTGCCAAGC CCCAGCTGGA GATGGTGACT GAGGCTACCA GTGACACATC CAAAGAGGTG 
CTCATGGTGG AGGTTGAGAA TGTAGCCCAT GAAGACTTTC AGGTCACAGA GGAAGTGAAG 
GCCCTGACTG CAGAGATCGT GAAGACCATT CGGGATATCA TCGCCCTGAA CCCTCTGTAC 
AGAGAGTCCG TGCTGCAGAT GATGCAGGCA GGCCAGCGTG TGGTGGACAA CCCCATCTAC 
TTGAGCGACA TGGGTGCCGC ACTCACTGGG GCTGAGTCCC ATGAGCTGCA AGATGTGCTG 
GAAGAAACAA ACATCCTTAA GCGGCTCTAT AAGGCCCTGT CTCTTTTGAA GAAGGAGTTC 
GAGTTGAGCA AGCTTCAACA GCGCCTGGGG CGTGAGGTGG AGGAGAAGAT CAAGCAGACG 
CACAGGAAGT ACCTGCTGCA GGAGCAGCTA AAGATCATCA AGAAAGAGTT GGGACTAGAG 
AAGGATGATA AAGATGCCAT TGAGGAGAAG TTCCGGGAAC GCTTAAAGGA GCTTGTGGTT 
CCCAAGCATG TGATGGACGT GGTAGATGAG GAGCTGAGCA AGCTGGCCCT ACTGGACAAC 
CACTCCTCTG AATTCAATGT CACTCGCAAC TACCTGGACT GGCTGACGTC CATCCCATGG 
GGCCGGCAGA GCGATGAGAA CTTAGACCTG GCTCGGGCCC AGTCGGTGCT GGAGGAGGAC 
CACTATGGCA TGGAGGATGT GAAGAAGCGA GTCTTGGAGT TCATTGCAGT CAGCCAGCTC 
CGAGGCTCCA CACAGGGCAA GATCCTCTGC TTCCACGGCC CACCAGGTGT GGGCAAGACC 
AGCATTGCAC GCTCCATTGC CCGTGCCCTT GGCCGTGAAT ACTTCCGCTT TAGTGTTGGT 
GGCATGACAG ATGTGGCAGA AATCAAGGGG CACAGGCGTA CCTATGTGGG GGCCATGCCT 
GGGAAGATTA TCCAGTGTCT GAAGAAAACC AAGACAGAGA ACCCATTAGT GCTGATTGAT 
GAGGTGGATA AGATTGGCCG AGGCTACCAA GGGGACCCGT CATCAGCACT GTTGGAGTTG 
TTGGACCCTG AGCAGAATGC CAACTTCCTG GACCACTACC TGGATGTGCC AGTGGACCTG 
TCCAAGGTGC TATTCATCTG CACAGCCAAC GTCACTGACA CTATACCGGA GCCACTAAGG 
GATCGCATGG AGATGATCAA TGTGTCAGGC TATGTAGCAC AGGAGAAGCT GGCCATTGCA 
GAGCGGTACC TGGTGCCACA GGCCCGCACC CTGTGTGGCC TAGACGAGAG CAAGGCCCAA 
CTGTCAGCGA CAGTGCTCAC TCTACTCATC AAACAGTACT GCAGGGAAAG TGGTGTGCGC 
AACCTGCAGA AGCAGGTGGA GAAGGTGCTG CGCAAGGCTG CATACAAGAT TGTAAGTGGA 
GAGGCCCAGA CAGTGCACGT GACACCTGAG AACCTGCAAG ACTTTGTAGG GAAGCCAGTG 
TTCACAGTGG AGCGCATGTA TGATGTCACG CCTCCTGGTG TGGTCATGGG TTTGGCCTGG 
ACTGCCATGG GAGGCTCCAC ATTGTTCGTG GAGACATCTT TAAGGAGGCC CCAGCCTAGT 
GGCAGCAAGG AGGACAAGGA TGGCAGCCTA GAGGTGACAG GTCAGCTAGG AGATGTCATG 
AAAGAGAGTG CCCGCATAGC CTACACCTTT GCCCGTGCCT TCCTGATGGA GCAGGACCCT 
GAAAATGACT TTCTGGTCAC ATCCCACATC CACCTGCATG TGCCTGAGGG CGCTACCCCC 
AAGGATGGCC CTAGTGCAGG TTGCACCATT GTCACTGCAT TGCTGTCCCT AGCTCTGGGG 
CAGCCAGTGT TGCAGAACCT GGCCATGACT GGGGAAGTCT CCCTCACTGG TAAAGTGTTG 
CCTGTGGGTG GCATCAAGGA GAAGACCATT GCGGCTAAGC GTGCGGGAGT GACCTGCATC 
ATTTTGCCTG CTGAGAACAG GAAGGACTTC TCAGACTTGG CTCCCTTCAT CACAGAGGGC 
CTGGAGGTTC ACTTCGTGGA ACACTACCGC GACATCTTCC GAATTGCCTT CCCCCTGCGA 
GAGCACCAGG AAGCACTGGC TGTGGAAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596895.1
CDS	2..2854
Translation	MAASTGYVRLWAAARCWVLRRPLLAVTGGRVPSASGSWLRRGCRVCDTSTPWGGRVPMGGGQWRGLWDAGSRGGSDETSEGGVEDGATASSGEGPVVTALAPMTVPDVFPHLPLIAISRNPVFPRFIKIVEVKNKKLVELLRRKVRLAQPYVGVFLKRDDNNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVTGHRRIHISRQLEVEPEGLEPEAENKQKSRRKLKRGKKEVGDELGAKPQLEMVTEATSDTSKEVLMVEVENVAHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNILKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDKDAIEEKFRERLKELVVPKHVMDVVDEELSKLALLDNHSSEFNVTRNYLDWLTSIPWGRQSDENLDLARAQSVLEEDHYGMEDVKKRVLEFIAVSQLRGSTQGKILCFHGPPGVGKTSIARSIARALGREYFRFSVGGMTDVAEIKGHRRTYVGAMPGKIIQCLKKTKTENPLVLIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARTLCGLDESKAQLSATVLTLLIKQYCRESGVRNLQKQVEKVLRKAAYKIVSGEAQTVHVTPENLQDFVGKPVFTVERMYDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQPSGSKEDKDGSLEVTGQLGDVMKESARIAYTFARAFLMEQDPENDFLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLALGQPVLQNLAMTGEVSLTGKVLPVGGIKEKTIAAKRAGVTCIILPAENRKDFSDLAPFITEGLEVHFVEHYRDIFRIAFPLREHQEALAVER

Target ORF information:

RefSeq Version	NM_133404.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lon peptidase 1, mitochondrial (Lonp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133404.1

ORF Insert Sequence:

ATGGCGGCAA GCACAGGTTA CGTGCGGCTG TGGGCTGCTG CGCGGTGCTG GGTTCTGCGG 
CGTCCGCTCT TGGCTGTCAC CGGAGGCCGA GTCCCGAGTG CGTCCGGGTC CTGGCTGCGC 
CGAGGATGTC GGGTCTGCGA CACGTCAACT CCCTGGGGCG GCCGCGTTCC TATGGGCGGG 
GGCCAGTGGC GGGGGCTGTG GGACGCGGGG AGCCGCGGTG GCAGCGACGA GACCTCCGAG 
GGCGGCGTGG AGGACGGGGC CACGGCGAGC AGCGGGGAAG GCCCGGTCGT CACGGCCCTC 
GCGCCTATGA CCGTCCCGGA TGTGTTTCCT CACCTGCCGC TCATCGCCAT CTCCCGCAAC 
CCGGTGTTCC CGCGCTTTAT CAAGATCGTG GAGGTTAAAA ATAAGAAGTT GGTTGAGCTG 
CTGAGAAGGA AAGTCCGCCT GGCACAGCCG TATGTTGGCG TCTTCTTAAA GAGAGATGAT 
AACAATGAGT CAGACGTGGT GGAGAGCCTG GATGAGATCT ACCATACGGG AACGTTCGCT 
CAGATTCATG AGATGCAGGA CCTCGGAGAC AAGTTGCGCA TGATCGTTAC AGGCCACAGA 
AGGATTCACA TCAGCCGGCA GCTGGAGGTG GAACCAGAGG GGCTGGAGCC AGAGGCAGAG 
AACAAACAAA AGTCCAGGAG GAAGCTGAAG CGAGGCAAGA AAGAGGTCGG GGATGAGCTG 
GGTGCCAAGC CCCAGCTGGA GATGGTGACT GAGGCTACCA GTGACACATC CAAAGAGGTG 
CTCATGGTGG AGGTTGAGAA TGTAGCCCAT GAAGACTTTC AGGTCACAGA GGAAGTGAAG 
GCCCTGACTG CAGAGATCGT GAAGACCATT CGGGATATCA TCGCCCTGAA CCCTCTGTAC 
AGAGAGTCCG TGCTGCAGAT GATGCAGGCA GGCCAGCGTG TGGTGGACAA CCCCATCTAC 
TTGAGCGACA TGGGTGCCGC ACTCACTGGG GCTGAGTCCC ATGAGCTGCA AGATGTGCTG 
GAAGAAACAA ACATCCTTAA GCGGCTCTAT AAGGCCCTGT CTCTTTTGAA GAAGGAGTTC 
GAGTTGAGCA AGCTTCAACA GCGCCTGGGG CGTGAGGTGG AGGAGAAGAT CAAGCAGACG 
CACAGGAAGT ACCTGCTGCA GGAGCAGCTA AAGATCATCA AGAAAGAGTT GGGACTAGAG 
AAGGATGATA AAGATGCCAT TGAGGAGAAG TTCCGGGAAC GCTTAAAGGA GCTTGTGGTT 
CCCAAGCATG TGATGGACGT GGTAGATGAG GAGCTGAGCA AGCTGGCCCT ACTGGACAAC 
CACTCCTCTG AATTCAATGT CACTCGCAAC TACCTGGACT GGCTGACGTC CATCCCATGG 
GGCCGGCAGA GCGATGAGAA CTTAGACCTG GCTCGGGCCC AGTCGGTGCT GGAGGAGGAC 
CACTATGGCA TGGAGGATGT GAAGAAGCGA GTCTTGGAGT TCATTGCAGT CAGCCAGCTC 
CGAGGCTCCA CACAGGGCAA GATCCTCTGC TTCCACGGCC CACCAGGTGT GGGCAAGACC 
AGCATTGCAC GCTCCATTGC CCGTGCCCTT GGCCGTGAAT ACTTCCGCTT TAGTGTTGGT 
GGCATGACAG ATGTGGCAGA AATCAAGGGG CACAGGCGTA CCTATGTGGG GGCCATGCCT 
GGGAAGATTA TCCAGTGTCT GAAGAAAACC AAGACAGAGA ACCCATTAGT GCTGATTGAT 
GAGGTGGATA AGATTGGCCG AGGCTACCAA GGGGACCCGT CATCAGCACT GTTGGAGTTG 
TTGGACCCTG AGCAGAATGC CAACTTCCTG GACCACTACC TGGATGTGCC AGTGGACCTG 
TCCAAGGTGC TATTCATCTG CACAGCCAAC GTCACTGACA CTATACCGGA GCCACTAAGG 
GATCGCATGG AGATGATCAA TGTGTCAGGC TATGTAGCAC AGGAGAAGCT GGCCATTGCA 
GAGCGGTACC TGGTGCCACA GGCCCGCACC CTGTGTGGCC TAGACGAGAG CAAGGCCCAA 
CTGTCAGCGA CAGTGCTCAC TCTACTCATC AAACAGTACT GCAGGGAAAG TGGTGTGCGC 
AACCTGCAGA AGCAGGTGGA GAAGGTGCTG CGCAAGGCTG CATACAAGAT TGTAAGTGGA 
GAGGCCCAGA CAGTGCACGT GACACCTGAG AACCTGCAAG ACTTTGTAGG GAAGCCAGTG 
TTCACAGTGG AGCGCATGTA TGATGTCACG CCTCCTGGTG TGGTCATGGG TTTGGCCTGG 
ACTGCCATGG GAGGCTCCAC ATTGTTCGTG GAGACATCTT TAAGGAGGCC CCAGCCTAGT 
GGCAGCAAGG AGGACAAGGA TGGCAGCCTA GAGGTGACAG GTCAGCTAGG AGATGTCATG 
AAAGAGAGTG CCCGCATAGC CTACACCTTT GCCCGTGCCT TCCTGATGGA GCAGGACCCT 
GAAAATGACT TTCTGGTCAC ATCCCACATC CACCTGCATG TGCCTGAGGG CGCTACCCCC 
AAGGATGGCC CTAGTGCAGG TTGCACCATT GTCACTGCAT TGCTGTCCCT AGCTCTGGGG 
CAGCCAGTGT TGCAGAACCT GGCCATGACT GGGGAAGTCT CCCTCACTGG TAAAGTGTTG 
CCTGTGGGTG GCATCAAGGA GAAGACCATT GCGGCTAAGC GTGCGGGAGT GACCTGCATC 
ATTTTGCCTG CTGAGAACAG GAAGGACTTC TCAGACTTGG CTCCCTTCAT CACAGAGGGC 
CTGGAGGTTC ACTTCGTGGA ACACTACCGC GACATCTTCC GAATTGCCTT CCCCCTGCGA 
GAGCACCAGG AAGCACTGGC TGTGGAAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Oxygen-sensitive mitochondrial accumulation of cystathionine ?-synthase mediated by Lon protease.
Proceedings of the National Academy of Sciences of the United States of America110(31)12679-84(2013 Jul)
Teng H,Wu B,Zhao K,Yang G,Wu L,Wang R

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Susceptibility of mitochondrial superoxide dismutase to aluminium induced oxidative damage.
Toxicology255(3)117-23(2009 Jan)
Kumar V,Bal A,Gill KD

Inactivation of brain mitochondrial Lon protease by peroxynitrite precedes electron transport chain dysfunction.
Neurochemistry international53(3-4)95-101(2008 Sep)
Stanyer L,Jorgensen W,Hori O,Clark JB,Heales SJ

Thermodynamic characterization of specific interactions between the human Lon protease and G-quartet DNA.
Nucleic acids research36(4)1273-87(2008 Mar)
Chen SH,Suzuki CK,Wu SH

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Lonp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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