Tas1r3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tas1r3
Entrez Gene ID	170634
Full Name	taste 1 receptor member 3
Synonyms	T1r3
General protein information	Preferred Names taste 1 receptor member 3 Names taste receptor type 1 member 3 sweet taste receptor T1R3 taste receptor, type 1, member 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	taste-specific G protein-coupled receptors which recognize natural and synthetic sweeteners [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_130818.1	NP_570831.1	taste receptor type 1 member 3 precursor
XM_008764334.1	XP_008762556.1	taste receptor type 1 member 3 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TAS1R3	XP_003307796.1
Gallus gallus (chicken)	TAS1R3	XP_425740.2
Danio rerio (zebrafish)	tas1r3	NP_001034717.1
Canis lupus familiaris (dog)	TAS1R3	NP_001026991.1
Mus musculus (house mouse)	Tas1r3	NP_114078.1
Rattus norvegicus (Norway rat)	Tas1r3	NP_570831.1
Homo sapiens (human)	TAS1R3	NP_689414.1
	TAS1R3	XP_001094084.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression of sweet taste receptor and gut hormone secretion in modelled type 2 diabetes.
Feng R, Qian C, Liu Q, Jin Y, Liu L, Li S, Liao Y, Zhou H, Liu W, Rayner CK, Ma J
General and comparative endocrinology252142-149(2017 Oct)

T1R1/T1R3 Taste Receptor Suppresses Granulocyte-Mediated Neuroinflammation after Spinal Cord Injury.
Shi J, Wang S, Ke Q, Lin J, Zheng Y, Wu S, Huang Z, Lin W
Journal of neurotrauma34(15)2353-2363(2017 Aug)

Involvement of glucocorticoid in induction of lingual T1R3 in rodents.
Ogawa N, Kanki K, Honda K, Tomooka Y, Ryoke K, Watanabe T
European journal of pharmacology761262-7(2015 Aug)

Expression of the glucose-sensing receptor T1R3 in pancreatic islet: changes in the expression levels in various nutritional and metabolic states.
Medina A, Nakagawa Y, Ma J, Li L, Hamano K, Akimoto T, Ninomiya Y, Kojima I
Endocrine journal61(8)797-805(2014)

REEP2 enhances sweet receptor function by recruitment to lipid rafts.
Ilegems E, Iwatsuki K, Kokrashvili Z, Benard O, Ninomiya Y, Margolskee RF
The Journal of neuroscience : the official journal of the Society for Neuroscience30(41)13774-83(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sweet taste molecules of T1R2, T1R3, alpha-gustducin and TRPM5 in ileum were dramatically higher than those in duodenum (P<0.01 for each).
Title: Expression of sweet taste receptor and gut hormone secretion in modelled type 2 diabetes.

Low concentration of endogenous GC is necessary and sufficient for induction of T1R3 expression. Higher concentrations may inhibit such induction. This inhibitory effect may be due, at least in part, to a direct action of GC on taste cells.
Title: Involvement of glucocorticoid in induction of lingual T1R3 in rodents.

T1R3 is expressed mainly in beta-cells and the expression levels are different depending upon the nutritional and metabolic conditions.
Title: Expression of the glucose-sensing receptor T1R3 in pancreatic islet: changes in the expression levels in various nutritional and metabolic states.

results suggest that nutritional status alters saccharin preference and the expression of T1R3 in taste buds; these processes may be involved in the mechanisms underlying the modulation of peripheral sweet taste sensitivity, in which leptin plays a role
Title: Nutritional status alters saccharin intake and sweet receptor mRNA expression in rat taste buds.

The following Tas1r3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tas1r3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00479	NM_130818.1 Latest version!	Rattus norvegicus taste 1 receptor member 3 (Tas1r3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa00479	XM_008764334.1 Latest version!	Rattus norvegicus taste 1 receptor member 3 (Tas1r3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tas1r3 ( NM_130818.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_570831.1 Rattus norvegicus taste 1 receptor member 3 (Tas1r3), mRNA.

Price & Availability↑

CloneID	ORa00479
Clone ID Related Accession (Same CDS sequence)	NM_130818.1 , XM_008764334.1
Accession Version	NM_130818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	taste receptor type 1 member 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY032620.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF456324.1, AY032620.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGGTT TGGCTATCTT GGGCCTCAGT CTGGCTGCTT TCCTGGAGCT TGGGATGGGG 
TCCTCTTTGT GTCTGTCACA GCAATTCAAG GCACAAGGGG ACTATATATT GGGTGGACTA 
TTTCCCCTGG GCACAACTGA GGAGGCCACT CTCAACCAGA GAACACAGCC CAACGGCATC 
CTATGTACCA GGTTCTCGCC CCTTGGTTTG TTCCTGGCCA TGGCTATGAA GATGGCTGTA 
GAGGAGATCA ACAATGGATC TGCCTTGCTC CCTGGGCTGC GACTGGGCTA TGACCTGTTT 
GACACATGCT CAGAGCCAGT GGTCACCATG AAGCCCAGCC TCATGTTCAT GGCCAAGGTG 
GGAAGTCAAA GCATTGCTGC CTACTGCAAC TACACACAGT ACCAACCCCG TGTGCTGGCT 
GTCATTGGTC CCCACTCATC AGAGCTTGCC CTCATTACAG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCAC AGGTCAGCTA TAGTGCCAGC ATGGATCGGC TAAGTGACCG GGAAACATTT 
CCATCCTTCT TCCGCACAGT GCCCAGTGAC CGGGTGCAGC TGCAGGCCGT TGTGACACTG 
TTGCAGAATT TCAGCTGGAA CTGGGTGGCT GCCTTAGGTA GTGATGATGA CTATGGCCGG 
GAAGGTCTGA GCATCTTTTC TGGTCTGGCC AACTCACGAG GTATCTGCAT TGCACACGAG 
GGCCTGGTGC CACAACATGA CACTAGTGGC CAACAATTGG GCAAGGTGGT GGATGTGCTA 
CGCCAAGTGA ACCAAAGCAA AGTACAGGTG GTGGTGCTGT TTGCATCTGC CCGTGCTGTC 
TACTCCCTTT TTAGCTACAG CATCCTTCAT GACCTCTCAC CCAAGGTATG GGTGGCCAGT 
GAGTCCTGGC TGACCTCTGA CCTGGTCATG ACACTTCCCA ATATTGCCCG TGTGGGCACT 
GTTCTTGGGT TTCTGCAGCG CGGTGCCCTA CTGCCTGAAT TTTCCCATTA TGTGGAGACT 
CGCCTTGCCC TAGCTGCTGA CCCAACATTC TGTGCCTCCC TGAAAGCTGA GTTGGATCTG 
GAGGAGCGCG TGATGGGGCC ACGCTGTTCA CAATGTGACT ACATCATGCT ACAGAACCTG 
TCATCTGGGC TGATGCAGAA CCTATCAGCT GGGCAGTTGC ACCACCAAAT ATTTGCAACC 
TATGCAGCTG TGTACAGTGT GGCTCAGGCC CTTCACAACA CCCTGCAGTG CAATGTCTCA 
CATTGCCACA CATCAGAGCC TGTTCAACCC TGGCAGCTCC TGGAGAACAT GTACAATATG 
AGTTTCCGTG CTCGAGACTT GACACTGCAG TTTGATGCCA AAGGGAGTGT AGACATGGAA 
TATGACCTGA AGATGTGGGT GTGGCAGAGC CCTACACCTG TACTACATAC TGTAGGCACC 
TTCAACGGCA CCCTTCAGCT GCAGCACTCG AAAATGTATT GGCCAGGCAA CCAGGTGCCA 
GTCTCCCAGT GCTCCCGGCA GTGCAAAGAT GGCCAGGTGC GCAGAGTAAA GGGCTTTCAT 
TCCTGCTGCT ATGACTGTGT GGACTGCAAG GCAGGGAGCT ACCGGAAGCA TCCAGATGAC 
TTCACCTGTA CTCCATGTGG CAAGGATCAG TGGTCCCCAG AAAAAAGCAC AACCTGCTTA 
CCTCGCAGGC CCAAGTTTCT GGCTTGGGGG GAGCCAGCTG TGCTGTCACT TCTCCTGCTG 
CTTTGCCTGG TGCTGGGCCT GACACTGGCT GCCCTGGGGC TCTTTGTCCA CTACTGGGAC 
AGCCCTCTTG TTCAGGCCTC AGGTGGGTCA CTGTTCTGCT TTGGCCTGAT CTGCCTAGGC 
CTCTTCTGCC TCAGTGTCCT TCTGTTCCCA GGACGACCAC GCTCTGCCAG CTGCCTTGCC 
CAACAACCAA TGGCTCACCT CCCTCTCACA GGCTGCCTGA GCACACTCTT CCTGCAAGCA 
GCCGAGATCT TTGTGGAGTC TGAGCTGCCA CTGAGTTGGG CAAACTGGCT CTGCAGCTAC 
CTTCGGGGCC CCTGGGCTTG GCTGGTGGTA CTGCTGGCCA CTCTTGTGGA GGCTGCACTA 
TGTGCCTGGT ACTTGATGGC TTTCCCTCCA GAGGTGGTGA CAGATTGGCA GGTGCTGCCC 
ACGGAGGTAC TGGAACACTG CCGCATGCGT TCCTGGGTCA GCCTGGGCTT GGTGCACATC 
ACCAATGCAG TGTTAGCTTT CCTCTGCTTT CTGGGCACTT TCCTGGTACA GAGCCAGCCT 
GGTCGCTATA ACCGTGCCCG TGGCCTCACC TTCGCCATGC TAGCTTATTT CATCATCTGG 
GTCTCTTTTG TGCCCCTCCT GGCTAATGTG CAGGTGGCCT ACCAGCCAGC TGTGCAGATG 
GGTGCTATCT TATTCTGTGC CCTGGGCATC CTGGCCACCT TCCACCTGCC CAAATGCTAT 
GTACTTCTGT GGCTGCCAGA GCTCAACACC CAGGAGTTCT TCCTGGGAAG GAGCCCCAAG 
GAAGCATCAG ATGGGAATAG TGGTAGTAGT GAGGCAACTC GGGGACACAG TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570831.1
CDS	1..2577
Translation	MPGLAILGLSLAAFLELGMGSSLCLSQQFKAQGDYILGGLFPLGTTEEATLNQRTQPNGILCTRFSPLGLFLAMAMKMAVEEINNGSALLPGLRLGYDLFDTCSEPVVTMKPSLMFMAKVGSQSIAAYCNYTQYQPRVLAVIGPHSSELALITGKFFSFFLMPQVSYSASMDRLSDRETFPSFFRTVPSDRVQLQAVVTLLQNFSWNWVAALGSDDDYGREGLSIFSGLANSRGICIAHEGLVPQHDTSGQQLGKVVDVLRQVNQSKVQVVVLFASARAVYSLFSYSILHDLSPKVWVASESWLTSDLVMTLPNIARVGTVLGFLQRGALLPEFSHYVETRLALAADPTFCASLKAELDLEERVMGPRCSQCDYIMLQNLSSGLMQNLSAGQLHHQIFATYAAVYSVAQALHNTLQCNVSHCHTSEPVQPWQLLENMYNMSFRARDLTLQFDAKGSVDMEYDLKMWVWQSPTPVLHTVGTFNGTLQLQHSKMYWPGNQVPVSQCSRQCKDGQVRRVKGFHSCCYDCVDCKAGSYRKHPDDFTCTPCGKDQWSPEKSTTCLPRRPKFLAWGEPAVLSLLLLLCLVLGLTLAALGLFVHYWDSPLVQASGGSLFCFGLICLGLFCLSVLLFPGRPRSASCLAQQPMAHLPLTGCLSTLFLQAAEIFVESELPLSWANWLCSYLRGPWAWLVVLLATLVEAALCAWYLMAFPPEVVTDWQVLPTEVLEHCRMRSWVSLGLVHITNAVLAFLCFLGTFLVQSQPGRYNRARGLTFAMLAYFIIWVSFVPLLANVQVAYQPAVQMGAILFCALGILATFHLPKCYVLLWLPELNTQEFFLGRSPKEASDGNSGSSEATRGHSE

Target ORF information:

RefSeq Version	NM_130818.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus taste 1 receptor member 3 (Tas1r3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130818.1

ORF Insert Sequence:

ATGCCGGGTT TGGCTATCTT GGGCCTCAGT CTGGCTGCTT TCCTGGAGCT TGGGATGGGG 
TCCTCTTTGT GTCTGTCACA GCAATTCAAG GCACAAGGGG ACTATATATT GGGTGGACTA 
TTTCCCCTGG GCACAACTGA GGAGGCCACT CTCAACCAGA GAACACAGCC CAACGGCATC 
CTATGTACCA GGTTCTCGCC CCTTGGTTTG TTCCTGGCCA TGGCTATGAA GATGGCTGTA 
GAGGAGATCA ACAATGGATC TGCCTTGCTC CCTGGGCTGC GACTGGGCTA TGACCTGTTT 
GACACATGCT CAGAGCCAGT GGTCACCATG AAGCCCAGCC TCATGTTCAT GGCCAAGGTG 
GGAAGTCAAA GCATTGCTGC CTACTGCAAC TACACACAGT ACCAACCCCG TGTGCTGGCT 
GTCATTGGTC CCCACTCATC AGAGCTTGCC CTCATTACAG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCAC AGGTCAGCTA TAGTGCCAGC ATGGATCGGC TAAGTGACCG GGAAACATTT 
CCATCCTTCT TCCGCACAGT GCCCAGTGAC CGGGTGCAGC TGCAGGCCGT TGTGACACTG 
TTGCAGAATT TCAGCTGGAA CTGGGTGGCT GCCTTAGGTA GTGATGATGA CTATGGCCGG 
GAAGGTCTGA GCATCTTTTC TGGTCTGGCC AACTCACGAG GTATCTGCAT TGCACACGAG 
GGCCTGGTGC CACAACATGA CACTAGTGGC CAACAATTGG GCAAGGTGGT GGATGTGCTA 
CGCCAAGTGA ACCAAAGCAA AGTACAGGTG GTGGTGCTGT TTGCATCTGC CCGTGCTGTC 
TACTCCCTTT TTAGCTACAG CATCCTTCAT GACCTCTCAC CCAAGGTATG GGTGGCCAGT 
GAGTCCTGGC TGACCTCTGA CCTGGTCATG ACACTTCCCA ATATTGCCCG TGTGGGCACT 
GTTCTTGGGT TTCTGCAGCG CGGTGCCCTA CTGCCTGAAT TTTCCCATTA TGTGGAGACT 
CGCCTTGCCC TAGCTGCTGA CCCAACATTC TGTGCCTCCC TGAAAGCTGA GTTGGATCTG 
GAGGAGCGCG TGATGGGGCC ACGCTGTTCA CAATGTGACT ACATCATGCT ACAGAACCTG 
TCATCTGGGC TGATGCAGAA CCTATCAGCT GGGCAGTTGC ACCACCAAAT ATTTGCAACC 
TATGCAGCTG TGTACAGTGT GGCTCAGGCC CTTCACAACA CCCTGCAGTG CAATGTCTCA 
CATTGCCACA CATCAGAGCC TGTTCAACCC TGGCAGCTCC TGGAGAACAT GTACAATATG 
AGTTTCCGTG CTCGAGACTT GACACTGCAG TTTGATGCCA AAGGGAGTGT AGACATGGAA 
TATGACCTGA AGATGTGGGT GTGGCAGAGC CCTACACCTG TACTACATAC TGTAGGCACC 
TTCAACGGCA CCCTTCAGCT GCAGCACTCG AAAATGTATT GGCCAGGCAA CCAGGTGCCA 
GTCTCCCAGT GCTCCCGGCA GTGCAAAGAT GGCCAGGTGC GCAGAGTAAA GGGCTTTCAT 
TCCTGCTGCT ATGACTGTGT GGACTGCAAG GCAGGGAGCT ACCGGAAGCA TCCAGATGAC 
TTCACCTGTA CTCCATGTGG CAAGGATCAG TGGTCCCCAG AAAAAAGCAC AACCTGCTTA 
CCTCGCAGGC CCAAGTTTCT GGCTTGGGGG GAGCCAGCTG TGCTGTCACT TCTCCTGCTG 
CTTTGCCTGG TGCTGGGCCT GACACTGGCT GCCCTGGGGC TCTTTGTCCA CTACTGGGAC 
AGCCCTCTTG TTCAGGCCTC AGGTGGGTCA CTGTTCTGCT TTGGCCTGAT CTGCCTAGGC 
CTCTTCTGCC TCAGTGTCCT TCTGTTCCCA GGACGACCAC GCTCTGCCAG CTGCCTTGCC 
CAACAACCAA TGGCTCACCT CCCTCTCACA GGCTGCCTGA GCACACTCTT CCTGCAAGCA 
GCCGAGATCT TTGTGGAGTC TGAGCTGCCA CTGAGTTGGG CAAACTGGCT CTGCAGCTAC 
CTTCGGGGCC CCTGGGCTTG GCTGGTGGTA CTGCTGGCCA CTCTTGTGGA GGCTGCACTA 
TGTGCCTGGT ACTTGATGGC TTTCCCTCCA GAGGTGGTGA CAGATTGGCA GGTGCTGCCC 
ACGGAGGTAC TGGAACACTG CCGCATGCGT TCCTGGGTCA GCCTGGGCTT GGTGCACATC 
ACCAATGCAG TGTTAGCTTT CCTCTGCTTT CTGGGCACTT TCCTGGTACA GAGCCAGCCT 
GGTCGCTATA ACCGTGCCCG TGGCCTCACC TTCGCCATGC TAGCTTATTT CATCATCTGG 
GTCTCTTTTG TGCCCCTCCT GGCTAATGTG CAGGTGGCCT ACCAGCCAGC TGTGCAGATG 
GGTGCTATCT TATTCTGTGC CCTGGGCATC CTGGCCACCT TCCACCTGCC CAAATGCTAT 
GTACTTCTGT GGCTGCCAGA GCTCAACACC CAGGAGTTCT TCCTGGGAAG GAGCCCCAAG 
GAAGCATCAG ATGGGAATAG TGGTAGTAGT GAGGCAACTC GGGGACACAG TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tas1r3 ( XM_008764334.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762556.1 Rattus norvegicus taste 1 receptor member 3 (Tas1r3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa00479
Clone ID Related Accession (Same CDS sequence)	NM_130818.1 , XM_008764334.1
Accession Version	XM_008764334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	taste receptor type 1 member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGTT TGGCTATCTT GGGCCTCAGT CTGGCTGCTT TCCTGGAGCT TGGGATGGGG 
TCCTCTTTGT GTCTGTCACA GCAATTCAAG GCACAAGGGG ACTATATATT GGGTGGACTA 
TTTCCCCTGG GCACAACTGA GGAGGCCACT CTCAACCAGA GAACACAGCC CAACGGCATC 
CTATGTACCA GGTTCTCGCC CCTTGGTTTG TTCCTGGCCA TGGCTATGAA GATGGCTGTA 
GAGGAGATCA ACAATGGATC TGCCTTGCTC CCTGGGCTGC GACTGGGCTA TGACCTGTTT 
GACACATGCT CAGAGCCAGT GGTCACCATG AAGCCCAGCC TCATGTTCAT GGCCAAGGTG 
GGAAGTCAAA GCATTGCTGC CTACTGCAAC TACACACAGT ACCAACCCCG TGTGCTGGCT 
GTCATTGGTC CCCACTCATC AGAGCTTGCC CTCATTACAG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCAC AGGTCAGCTA TAGTGCCAGC ATGGATCGGC TAAGTGACCG GGAAACATTT 
CCATCCTTCT TCCGCACAGT GCCCAGTGAC CGGGTGCAGC TGCAGGCCGT TGTGACACTG 
TTGCAGAATT TCAGCTGGAA CTGGGTGGCT GCCTTAGGTA GTGATGATGA CTATGGCCGG 
GAAGGTCTGA GCATCTTTTC TGGTCTGGCC AACTCACGAG GTATCTGCAT TGCACACGAG 
GGCCTGGTGC CACAACATGA CACTAGTGGC CAACAATTGG GCAAGGTGGT GGATGTGCTA 
CGCCAAGTGA ACCAAAGCAA AGTACAGGTG GTGGTGCTGT TTGCATCTGC CCGTGCTGTC 
TACTCCCTTT TTAGCTACAG CATCCTTCAT GACCTCTCAC CCAAGGTATG GGTGGCCAGT 
GAGTCCTGGC TGACCTCTGA CCTGGTCATG ACACTTCCCA ATATTGCCCG TGTGGGCACT 
GTTCTTGGGT TTCTGCAGCG CGGTGCCCTA CTGCCTGAAT TTTCCCATTA TGTGGAGACT 
CGCCTTGCCC TAGCTGCTGA CCCAACATTC TGTGCCTCCC TGAAAGCTGA GTTGGATCTG 
GAGGAGCGCG TGATGGGGCC ACGCTGTTCA CAATGTGACT ACATCATGCT ACAGAACCTG 
TCATCTGGGC TGATGCAGAA CCTATCAGCT GGGCAGTTGC ACCACCAAAT ATTTGCAACC 
TATGCAGCTG TGTACAGTGT GGCTCAGGCC CTTCACAACA CCCTGCAGTG CAATGTCTCA 
CATTGCCACA CATCAGAGCC TGTTCAACCC TGGCAGCTCC TGGAGAACAT GTACAATATG 
AGTTTCCGTG CTCGAGACTT GACACTGCAG TTTGATGCCA AAGGGAGTGT AGACATGGAA 
TATGACCTGA AGATGTGGGT GTGGCAGAGC CCTACACCTG TACTACATAC TGTAGGCACC 
TTCAACGGCA CCCTTCAGCT GCAGCACTCG AAAATGTATT GGCCAGGCAA CCAGGTGCCA 
GTCTCCCAGT GCTCCCGGCA GTGCAAAGAT GGCCAGGTGC GCAGAGTAAA GGGCTTTCAT 
TCCTGCTGCT ATGACTGTGT GGACTGCAAG GCAGGGAGCT ACCGGAAGCA TCCAGATGAC 
TTCACCTGTA CTCCATGTGG CAAGGATCAG TGGTCCCCAG AAAAAAGCAC AACCTGCTTA 
CCTCGCAGGC CCAAGTTTCT GGCTTGGGGG GAGCCAGCTG TGCTGTCACT TCTCCTGCTG 
CTTTGCCTGG TGCTGGGCCT GACACTGGCT GCCCTGGGGC TCTTTGTCCA CTACTGGGAC 
AGCCCTCTTG TTCAGGCCTC AGGTGGGTCA CTGTTCTGCT TTGGCCTGAT CTGCCTAGGC 
CTCTTCTGCC TCAGTGTCCT TCTGTTCCCA GGACGACCAC GCTCTGCCAG CTGCCTTGCC 
CAACAACCAA TGGCTCACCT CCCTCTCACA GGCTGCCTGA GCACACTCTT CCTGCAAGCA 
GCCGAGATCT TTGTGGAGTC TGAGCTGCCA CTGAGTTGGG CAAACTGGCT CTGCAGCTAC 
CTTCGGGGCC CCTGGGCTTG GCTGGTGGTA CTGCTGGCCA CTCTTGTGGA GGCTGCACTA 
TGTGCCTGGT ACTTGATGGC TTTCCCTCCA GAGGTGGTGA CAGATTGGCA GGTGCTGCCC 
ACGGAGGTAC TGGAACACTG CCGCATGCGT TCCTGGGTCA GCCTGGGCTT GGTGCACATC 
ACCAATGCAG TGTTAGCTTT CCTCTGCTTT CTGGGCACTT TCCTGGTACA GAGCCAGCCT 
GGTCGCTATA ACCGTGCCCG TGGCCTCACC TTCGCCATGC TAGCTTATTT CATCATCTGG 
GTCTCTTTTG TGCCCCTCCT GGCTAATGTG CAGGTGGCCT ACCAGCCAGC TGTGCAGATG 
GGTGCTATCT TATTCTGTGC CCTGGGCATC CTGGCCACCT TCCACCTGCC CAAATGCTAT 
GTACTTCTGT GGCTGCCAGA GCTCAACACC CAGGAGTTCT TCCTGGGAAG GAGCCCCAAG 
GAAGCATCAG ATGGGAATAG TGGTAGTAGT GAGGCAACTC GGGGACACAG TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762556.1
CDS	797..3373
Translation	MPGLAILGLSLAAFLELGMGSSLCLSQQFKAQGDYILGGLFPLGTTEEATLNQRTQPNGILCTRFSPLGLFLAMAMKMAVEEINNGSALLPGLRLGYDLFDTCSEPVVTMKPSLMFMAKVGSQSIAAYCNYTQYQPRVLAVIGPHSSELALITGKFFSFFLMPQVSYSASMDRLSDRETFPSFFRTVPSDRVQLQAVVTLLQNFSWNWVAALGSDDDYGREGLSIFSGLANSRGICIAHEGLVPQHDTSGQQLGKVVDVLRQVNQSKVQVVVLFASARAVYSLFSYSILHDLSPKVWVASESWLTSDLVMTLPNIARVGTVLGFLQRGALLPEFSHYVETRLALAADPTFCASLKAELDLEERVMGPRCSQCDYIMLQNLSSGLMQNLSAGQLHHQIFATYAAVYSVAQALHNTLQCNVSHCHTSEPVQPWQLLENMYNMSFRARDLTLQFDAKGSVDMEYDLKMWVWQSPTPVLHTVGTFNGTLQLQHSKMYWPGNQVPVSQCSRQCKDGQVRRVKGFHSCCYDCVDCKAGSYRKHPDDFTCTPCGKDQWSPEKSTTCLPRRPKFLAWGEPAVLSLLLLLCLVLGLTLAALGLFVHYWDSPLVQASGGSLFCFGLICLGLFCLSVLLFPGRPRSASCLAQQPMAHLPLTGCLSTLFLQAAEIFVESELPLSWANWLCSYLRGPWAWLVVLLATLVEAALCAWYLMAFPPEVVTDWQVLPTEVLEHCRMRSWVSLGLVHITNAVLAFLCFLGTFLVQSQPGRYNRARGLTFAMLAYFIIWVSFVPLLANVQVAYQPAVQMGAILFCALGILATFHLPKCYVLLWLPELNTQEFFLGRSPKEASDGNSGSSEATRGHSE

Target ORF information:

RefSeq Version	XM_008764334.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus taste 1 receptor member 3 (Tas1r3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764334.1

ORF Insert Sequence:

ATGCCGGGTT TGGCTATCTT GGGCCTCAGT CTGGCTGCTT TCCTGGAGCT TGGGATGGGG 
TCCTCTTTGT GTCTGTCACA GCAATTCAAG GCACAAGGGG ACTATATATT GGGTGGACTA 
TTTCCCCTGG GCACAACTGA GGAGGCCACT CTCAACCAGA GAACACAGCC CAACGGCATC 
CTATGTACCA GGTTCTCGCC CCTTGGTTTG TTCCTGGCCA TGGCTATGAA GATGGCTGTA 
GAGGAGATCA ACAATGGATC TGCCTTGCTC CCTGGGCTGC GACTGGGCTA TGACCTGTTT 
GACACATGCT CAGAGCCAGT GGTCACCATG AAGCCCAGCC TCATGTTCAT GGCCAAGGTG 
GGAAGTCAAA GCATTGCTGC CTACTGCAAC TACACACAGT ACCAACCCCG TGTGCTGGCT 
GTCATTGGTC CCCACTCATC AGAGCTTGCC CTCATTACAG GCAAGTTCTT CAGCTTCTTC 
CTCATGCCAC AGGTCAGCTA TAGTGCCAGC ATGGATCGGC TAAGTGACCG GGAAACATTT 
CCATCCTTCT TCCGCACAGT GCCCAGTGAC CGGGTGCAGC TGCAGGCCGT TGTGACACTG 
TTGCAGAATT TCAGCTGGAA CTGGGTGGCT GCCTTAGGTA GTGATGATGA CTATGGCCGG 
GAAGGTCTGA GCATCTTTTC TGGTCTGGCC AACTCACGAG GTATCTGCAT TGCACACGAG 
GGCCTGGTGC CACAACATGA CACTAGTGGC CAACAATTGG GCAAGGTGGT GGATGTGCTA 
CGCCAAGTGA ACCAAAGCAA AGTACAGGTG GTGGTGCTGT TTGCATCTGC CCGTGCTGTC 
TACTCCCTTT TTAGCTACAG CATCCTTCAT GACCTCTCAC CCAAGGTATG GGTGGCCAGT 
GAGTCCTGGC TGACCTCTGA CCTGGTCATG ACACTTCCCA ATATTGCCCG TGTGGGCACT 
GTTCTTGGGT TTCTGCAGCG CGGTGCCCTA CTGCCTGAAT TTTCCCATTA TGTGGAGACT 
CGCCTTGCCC TAGCTGCTGA CCCAACATTC TGTGCCTCCC TGAAAGCTGA GTTGGATCTG 
GAGGAGCGCG TGATGGGGCC ACGCTGTTCA CAATGTGACT ACATCATGCT ACAGAACCTG 
TCATCTGGGC TGATGCAGAA CCTATCAGCT GGGCAGTTGC ACCACCAAAT ATTTGCAACC 
TATGCAGCTG TGTACAGTGT GGCTCAGGCC CTTCACAACA CCCTGCAGTG CAATGTCTCA 
CATTGCCACA CATCAGAGCC TGTTCAACCC TGGCAGCTCC TGGAGAACAT GTACAATATG 
AGTTTCCGTG CTCGAGACTT GACACTGCAG TTTGATGCCA AAGGGAGTGT AGACATGGAA 
TATGACCTGA AGATGTGGGT GTGGCAGAGC CCTACACCTG TACTACATAC TGTAGGCACC 
TTCAACGGCA CCCTTCAGCT GCAGCACTCG AAAATGTATT GGCCAGGCAA CCAGGTGCCA 
GTCTCCCAGT GCTCCCGGCA GTGCAAAGAT GGCCAGGTGC GCAGAGTAAA GGGCTTTCAT 
TCCTGCTGCT ATGACTGTGT GGACTGCAAG GCAGGGAGCT ACCGGAAGCA TCCAGATGAC 
TTCACCTGTA CTCCATGTGG CAAGGATCAG TGGTCCCCAG AAAAAAGCAC AACCTGCTTA 
CCTCGCAGGC CCAAGTTTCT GGCTTGGGGG GAGCCAGCTG TGCTGTCACT TCTCCTGCTG 
CTTTGCCTGG TGCTGGGCCT GACACTGGCT GCCCTGGGGC TCTTTGTCCA CTACTGGGAC 
AGCCCTCTTG TTCAGGCCTC AGGTGGGTCA CTGTTCTGCT TTGGCCTGAT CTGCCTAGGC 
CTCTTCTGCC TCAGTGTCCT TCTGTTCCCA GGACGACCAC GCTCTGCCAG CTGCCTTGCC 
CAACAACCAA TGGCTCACCT CCCTCTCACA GGCTGCCTGA GCACACTCTT CCTGCAAGCA 
GCCGAGATCT TTGTGGAGTC TGAGCTGCCA CTGAGTTGGG CAAACTGGCT CTGCAGCTAC 
CTTCGGGGCC CCTGGGCTTG GCTGGTGGTA CTGCTGGCCA CTCTTGTGGA GGCTGCACTA 
TGTGCCTGGT ACTTGATGGC TTTCCCTCCA GAGGTGGTGA CAGATTGGCA GGTGCTGCCC 
ACGGAGGTAC TGGAACACTG CCGCATGCGT TCCTGGGTCA GCCTGGGCTT GGTGCACATC 
ACCAATGCAG TGTTAGCTTT CCTCTGCTTT CTGGGCACTT TCCTGGTACA GAGCCAGCCT 
GGTCGCTATA ACCGTGCCCG TGGCCTCACC TTCGCCATGC TAGCTTATTT CATCATCTGG 
GTCTCTTTTG TGCCCCTCCT GGCTAATGTG CAGGTGGCCT ACCAGCCAGC TGTGCAGATG 
GGTGCTATCT TATTCTGTGC CCTGGGCATC CTGGCCACCT TCCACCTGCC CAAATGCTAT 
GTACTTCTGT GGCTGCCAGA GCTCAACACC CAGGAGTTCT TCCTGGGAAG GAGCCCCAAG 
GAAGCATCAG ATGGGAATAG TGGTAGTAGT GAGGCAACTC GGGGACACAG TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of sweet taste receptor and gut hormone secretion in modelled type 2 diabetes.
General and comparative endocrinology252142-149(2017 Oct)
Feng R,Qian C,Liu Q,Jin Y,Liu L,Li S,Liao Y,Zhou H,Liu W,Rayner CK,Ma J

T1R1/T1R3 Taste Receptor Suppresses Granulocyte-Mediated Neuroinflammation after Spinal Cord Injury.
Journal of neurotrauma34(15)2353-2363(2017 Aug)
Shi J,Wang S,Ke Q,Lin J,Zheng Y,Wu S,Huang Z,Lin W

Involvement of glucocorticoid in induction of lingual T1R3 in rodents.
European journal of pharmacology761262-7(2015 Aug)
Ogawa N,Kanki K,Honda K,Tomooka Y,Ryoke K,Watanabe T

Expression of the glucose-sensing receptor T1R3 in pancreatic islet: changes in the expression levels in various nutritional and metabolic states.
Endocrine journal61(8)797-805(2014)
Medina A,Nakagawa Y,Ma J,Li L,Hamano K,Akimoto T,Ninomiya Y,Kojima I

REEP2 enhances sweet receptor function by recruitment to lipid rafts.
The Journal of neuroscience : the official journal of the Society for Neuroscience30(41)13774-83(2010 Oct)
Ilegems E,Iwatsuki K,Kokrashvili Z,Benard O,Ninomiya Y,Margolskee RF

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Tas1r3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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