Acot8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acot8
Entrez Gene ID	170588
Full Name	acyl-CoA thioesterase 8
Synonyms	Pte1
General protein information	Preferred Names acyl-CoA thioesterase 8 Names acyl-coenzyme A thioesterase 8 4,8-dimethylnonanoyl-CoA thioesterase 48-dimethylnonanoyl-CoA thioesterase PTE-1 PTE-2 choloyl-coenzyme A thioesterase peroxisomal acyl-CoA thioesterase 1 peroxisomal acyl-CoA thioesterase 2 peroxisomal acyl-coenzyme A thioester hydrolase 1 peroxisomal long-chain acyl-CoA thioesterase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q42
Summary	thioesterase; involved in conversion of dimethylnonanoyl-CoA and dimethylheptanoyl-CoA into 4,8-dimethylnonanoic acid plus CoASH [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_130756.3	NP_570112.3	acyl-coenzyme A thioesterase 8

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACOT8	NP_005460.2
	ACOT8	XP_001108571.2
Caenorhabditis elegans (roundworm)	CELE_C17C3.3	NP_495072.2
Bos taurus (cattle)	ACOT8	NP_001068625.2
Caenorhabditis elegans (roundworm)	F25E2.3	NP_508159.2
Canis lupus familiaris (dog)	ACOT8	XP_534440.1
Mus musculus (house mouse)	Acot8	NP_573503.2
Gallus gallus (chicken)	ACOT8	XP_417475.3
Caenorhabditis elegans (roundworm)	CELE_C17C3.1	NP_495075.1
Arabidopsis thaliana (thale cress)	AT4G00520	NP_001078340.1
Pan troglodytes (chimpanzee)	ACOT8	XP_001158053.1
Rattus norvegicus (Norway rat)	Acot8	NP_570112.3
Danio rerio (zebrafish)	acot8	NP_001006072.1
Arabidopsis thaliana (thale cress)	AT1G01710	NP_563632.2
Xenopus tropicalis (tropical clawed frog)	acot8	XP_002932556.1
Caenorhabditis elegans (roundworm)	C37H5.13	NP_504301.1

Gene Ontology
Bibliography

Related articles in PubMed

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Ghosh D, Berg JM
Journal of the American Chemical Society132(11)3973-9(2010 Mar)

The identification of a succinyl-CoA thioesterase suggests a novel pathway for succinate production in peroxisomes.
Westin MA, Hunt MC, Alexson SE
The Journal of biological chemistry280(46)38125-32(2005 Nov)

A revised nomenclature for mammalian acyl-CoA thioesterases/hydrolases.
Hunt MC, Yamada J, Maltais LJ, Wright MW, Podesta EJ, Alexson SE
Journal of lipid research46(9)2029-32(2005 Sep)

Overexpression of human acyl-CoA thioesterase upregulates peroxisome biogenesis.
Ishizuka M, Toyama Y, Watanabe H, Fujiki Y, Takeuchi A, Yamasaki S, Yuasa S, Miyazaki M, Nakajima N, Taki S, Saito T
Experimental cell research297(1)127-41(2004 Jul)

PEX19 is a predominantly cytosolic chaperone and import receptor for class 1 peroxisomal membrane proteins.
Jones JM, Morrell JC, Gould SJ
The Journal of cell biology164(1)57-67(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Acot8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acot8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00466	NM_130756.3 Latest version!	Rattus norvegicus acyl-CoA thioesterase 8 (Acot8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acot8 ( NM_130756.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_570112.3 Rattus norvegicus acyl-CoA thioesterase 8 (Acot8), mRNA.

Price & Availability↑

CloneID	ORa00466
Clone ID Related Accession (Same CDS sequence)	NM_130756.3
Accession Version	NM_130756.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-15
Organism	Rattus norvegicus(Norway rat)
Product	acyl-coenzyme A thioesterase 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CV115391.1 and BC078751.1. On Sep 28, 2011 this sequence version replaced NM_130756.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078751.1, FQ212945.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMN01906352 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACAC CCACTCAGAA ATTGACAAGC ACAACGACCA ATCAGAATCT GCGTCTCGCA 
CACGGAAACC CTAGGGATTT CCGGCCCCTG GAGCGACCTG CCGGAAGCGC TGGGTGCCTA 
TATAAGTTTC CGCGTTTCTC TCATCTGCAG CAAGCAGTGA AAATGTCAAA GCCAGAGGAC 
CTAGGAGATG CTAATGGCGA CGCCGACCGC GGCGACCTTT CCGGGGACCT GCGTAGTGTG 
CTGGTCACCA GCGTGCTCAA CCTCGAGCCT CTAGATGAAG ATCTCTACAG AGGAAGGCAT 
TACTGGGTAC CTACCTCCCA GCGGCTCTTT GGGGGTCAGA TTGTGGGCCA GGCTCTGGTG 
GCTGCAGCCA AGTCCGTGAG TGAAGACGTC CACGTGCACT CCCTGCACTG CTACTTTGTC 
CGGGCAGGGG ACCCAAAGGT GCCAGTGCTG TACCATGTGG AGAGGACACG AACAGGAGCA 
AGCTTCTCAG TGCGTGCAGT GAAGGCTGTG CAGCATGGCA AGGCCATCTT CATCTGCCAG 
GCCTCCTTCC AGCAGATGCA GCCCAGCCCC CTGCAGCACC AGTTCTCCAT GCCCACTGTG 
CCCCCCCCAG AGGAGCTGCT GGACCATGAG GCCCTCATTG ACCAGTACTT AAGGGATCCT 
AACCTTCATG AGAAATATCG AGTGGGGCTG AACCGAATTG CTGCCCGAGA GGTGCCTATT 
GAGATCAAGC TGGTGAACCC ACCCGCCCTG AACCAGCTGC AGACACTGGA GCCCAAACAG 
ATGTTCTGGG TGCGAGCCCG GGGCTATATT GGGGACGGCG ACATCAAGAT GCACTGCTGT 
GTGGCTGCTT ACATCTCTGA CTACGCCTTC CTGGGGACGG CACTGCTGCC TCACCAGTCC 
AAGTATAAAG TGAACTTCAT GGTCTCGCTG GACCACTCCA TGTGGTTCCA CGCCCCATTC 
CGTGCTGACC ACTGGATGCT CTACGAGTGT GAGAGCCCCT GGGCTGGTGG CTCTCGAGGG 
CTGGTACATG GGCGGCTGTG GCGTCGGGAT GGGGTCCTTG CTGTGACCTG TGCCCAGGAG 
GGTGTGATCC GATCGAAGCC TCGGGTATCC GAGAGTAAAC TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570112.3
CDS	231..1355
Translation	MATPTQKLTSTTTNQNLRLAHGNPRDFRPLERPAGSAGCLYKFPRFSHLQQAVKMSKPEDLGDANGDADRGDLSGDLRSVLVTSVLNLEPLDEDLYRGRHYWVPTSQRLFGGQIVGQALVAAAKSVSEDVHVHSLHCYFVRAGDPKVPVLYHVERTRTGASFSVRAVKAVQHGKAIFICQASFQQMQPSPLQHQFSMPTVPPPEELLDHEALIDQYLRDPNLHEKYRVGLNRIAAREVPIEIKLVNPPALNQLQTLEPKQMFWVRARGYIGDGDIKMHCCVAAYISDYAFLGTALLPHQSKYKVNFMVSLDHSMWFHAPFRADHWMLYECESPWAGGSRGLVHGRLWRRDGVLAVTCAQEGVIRSKPRVSESKL

Target ORF information:

RefSeq Version	NM_130756.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA thioesterase 8 (Acot8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130756.3

ORF Insert Sequence:

ATGGCCACAC CCACTCAGAA ATTGACAAGC ACAACGACCA ATCAGAATCT GCGTCTCGCA 
CACGGAAACC CTAGGGATTT CCGGCCCCTG GAGCGACCTG CCGGAAGCGC TGGGTGCCTA 
TATAAGTTTC CGCGTTTCTC TCATCTGCAG CAAGCAGTGA AAATGTCAAA GCCAGAGGAC 
CTAGGAGATG CTAATGGCGA CGCCGACCGC GGCGACCTTT CCGGGGACCT GCGTAGTGTG 
CTGGTCACCA GCGTGCTCAA CCTCGAGCCT CTAGATGAAG ATCTCTACAG AGGAAGGCAT 
TACTGGGTAC CTACCTCCCA GCGGCTCTTT GGGGGTCAGA TTGTGGGCCA GGCTCTGGTG 
GCTGCAGCCA AGTCCGTGAG TGAAGACGTC CACGTGCACT CCCTGCACTG CTACTTTGTC 
CGGGCAGGGG ACCCAAAGGT GCCAGTGCTG TACCATGTGG AGAGGACACG AACAGGAGCA 
AGCTTCTCAG TGCGTGCAGT GAAGGCTGTG CAGCATGGCA AGGCCATCTT CATCTGCCAG 
GCCTCCTTCC AGCAGATGCA GCCCAGCCCC CTGCAGCACC AGTTCTCCAT GCCCACTGTG 
CCCCCCCCAG AGGAGCTGCT GGACCATGAG GCCCTCATTG ACCAGTACTT AAGGGATCCT 
AACCTTCATG AGAAATATCG AGTGGGGCTG AACCGAATTG CTGCCCGAGA GGTGCCTATT 
GAGATCAAGC TGGTGAACCC ACCCGCCCTG AACCAGCTGC AGACACTGGA GCCCAAACAG 
ATGTTCTGGG TGCGAGCCCG GGGCTATATT GGGGACGGCG ACATCAAGAT GCACTGCTGT 
GTGGCTGCTT ACATCTCTGA CTACGCCTTC CTGGGGACGG CACTGCTGCC TCACCAGTCC 
AAGTATAAAG TGAACTTCAT GGTCTCGCTG GACCACTCCA TGTGGTTCCA CGCCCCATTC 
CGTGCTGACC ACTGGATGCT CTACGAGTGT GAGAGCCCCT GGGCTGGTGG CTCTCGAGGG 
CTGGTACATG GGCGGCTGTG GCGTCGGGAT GGGGTCCTTG CTGTGACCTG TGCCCAGGAG 
GGTGTGATCC GATCGAAGCC TCGGGTATCC GAGAGTAAAC TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Journal of the American Chemical Society132(11)3973-9(2010 Mar)
Ghosh D,Berg JM

The identification of a succinyl-CoA thioesterase suggests a novel pathway for succinate production in peroxisomes.
The Journal of biological chemistry280(46)38125-32(2005 Nov)
Westin MA,Hunt MC,Alexson SE

A revised nomenclature for mammalian acyl-CoA thioesterases/hydrolases.
Journal of lipid research46(9)2029-32(2005 Sep)
Hunt MC,Yamada J,Maltais LJ,Wright MW,Podesta EJ,Alexson SE

Overexpression of human acyl-CoA thioesterase upregulates peroxisome biogenesis.
Experimental cell research297(1)127-41(2004 Jul)
Ishizuka M,Toyama Y,Watanabe H,Fujiki Y,Takeuchi A,Yamasaki S,Yuasa S,Miyazaki M,Nakajima N,Taki S,Saito T

PEX19 is a predominantly cytosolic chaperone and import receptor for class 1 peroxisomal membrane proteins.
The Journal of cell biology164(1)57-67(2004 Jan)
Jones JM,Morrell JC,Gould SJ