Sdha cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sdha
Entrez Gene ID	157074
Full Name	succinate dehydrogenase complex flavoprotein subunit A
General protein information	Preferred Names succinate dehydrogenase complex flavoprotein subunit A Names succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial flavoprotein subunit of complex II fp succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1p11
Summary	subunit of the mitochondrial succinate hehydrogenase that is involved in the oxidation of succinate; mutation of human homolog is associated with Leigh syndrome [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_130428.1	NP_569112.1	succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SDHA	NP_001264327.1
Drosophila melanogaster (fruit fly)	SdhA	NP_725882.1
Eremothecium gossypii	AGOS_ACR052W	NP_983455.2
Schizosaccharomyces pombe (fission yeast)	sdh1	NP_594319.1
Oryza sativa (rice)	Os07g0134800	NP_001058845.1
Bos taurus (cattle)	SDHA	NP_776603.1
Mus musculus (house mouse)	Sdha	NP_075770.1
Rattus norvegicus (Norway rat)	Sdha	NP_569112.1
Danio rerio (zebrafish)	sdha	NP_957204.1
Caenorhabditis elegans (roundworm)	sdha-2	NP_492798.1
Arabidopsis thaliana (thale cress)	SDH1-1	NP_201477.1
Saccharomyces cerevisiae (baker's yeast)	SDH1	NP_012774.1
Kluyveromyces lactis	KLLA0D04444g	XP_453260.1
Neurospora crassa	NCU08336	XP_001728186.1
Pan troglodytes (chimpanzee)	SDHA	XP_003310656.1
	LOC708018	XP_001094170.2
Canis lupus familiaris (dog)	SDHA	XP_535807.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010429	XP_311518.4
Magnaporthe oryzae (rice blast fungus)	MGG_00168	XP_003718957.1
Xenopus tropicalis (tropical clawed frog)	sdha	NP_001015989.1
Homo sapiens (human)	SDHA	NP_004159.2
Caenorhabditis elegans (roundworm)	sdha-1	NP_509446.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondrial DNA Depletion in Respiratory Chain-Deficient Parkinson Disease Neurons.
Gr?newald A, Rygiel KA, Hepplewhite PD, Morris CM, Picard M, Turnbull DM
Annals of neurology79(3)366-78(2016 Mar)

Skeletal muscle Sirt3 expression and mitochondrial respiration are regulated by a prenatal low-protein diet.
Claycombe KJ, Roemmich JN, Johnson L, Vomhof-DeKrey EE, Johnson WT
The Journal of nutritional biochemistry26(2)184-9(2015 Feb)

SDHA mutations causing a multisystem mitochondrial disease: novel mutations and genetic overlap with hereditary tumors.
Renkema GH, Wortmann SB, Smeets RJ, Venselaar H, Antoine M, Visser G, Ben-Omran T, van den Heuvel LP, Timmers HJ, Smeitink JA, Rodenburg RJ
European journal of human genetics : EJHG23(2)202-9(2015 Feb)

Acute administration of l-tyrosine alters energetic metabolism of hippocampus and striatum of infant rats.
Ramos AC, Ferreira GK, Carvalho-Silva M, Furlanetto CB, Gon?alves CL, Ferreira GC, Schuck PF, Streck EL
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience31(5)303-7(2013 Aug)

Intraabdominal adhesion formation is associated with differential mRNA expression of metabolic genes PDHb and SDHa.
Wallwiener M, Wallwiener CW, Molinas R, Rajab TK, Brucker SY, Kraemer B, Hartkopf AD
Archives of gynecology and obstetrics286(3)683-6(2012 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest activation/acetylation of Sdha in skeletal muscle can be regulated by diet; maternal low-protein leads to inactivation of Sdha; postnatal high-fat leads to further inactivation of Sdha; this increases susceptibility to type 2 diabetes.
Title: Skeletal muscle Sirt3 expression and mitochondrial respiration are regulated by a prenatal low-protein diet.

Our results indicate an alteration in the energy metabolism in hippocampus and striatum of infant rats after acute administration of l-tyrosine.
Title: Acute administration of l-tyrosine alters energetic metabolism of hippocampus and striatum of infant rats.

up-regulated by 24 % in peritoneal adhesions
Title: Intraabdominal adhesion formation is associated with differential mRNA expression of metabolic genes PDHb and SDHa.

Data show that MCT-KD intake partially recovers age-related decrease of SDH activity and increases the myocardial area occupied by metabolically active mitochondria.
Title: A ketogenic diet increases succinic dehydrogenase activity in aging cardiomyocytes.

SDHa flavoprotein transcriptional regulation by nuclear respiratory factor-1 prevents pseudo-hypoxia in aerobic cardiac cells
Title: Transcriptional Regulation of SDHa flavoprotein by nuclear respiratory factor-1 prevents pseudo-hypoxia in aerobic cardiac cells.

The following Sdha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sdha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12479	NM_130428.1 Latest version!	Rattus norvegicus succinate dehydrogenase complex flavoprotein subunit A (Sdha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sdha ( NM_130428.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_569112.1 Rattus norvegicus succinate dehydrogenase complex flavoprotein subunit A (Sdha), mRNA.

Price & Availability↑

CloneID	ORa12479
Clone ID Related Accession (Same CDS sequence)	NM_130428.1
Accession Version	NM_130428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB072907.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB072907.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCCGGGG TTGGCGCAGT TTCGAGACTT CTTCGCGGGC GGCGCTTGGC TCTAGCTGGG 
GCGACTCGTG GCTTTCACTT CTCTGTTGGT GAGAGCAAGA AGGCATCCGC TAAAGTTTCA 
GACGCGATTT CTACCCAGTA CCCCGTGGTG GACCATGAGT TTGATGCTGT GGTTGTAGGT 
GCAGGCGGGG CAGGCTTGCG AGCTGCATTC GGCCTTTCTG AGGCAGGCTT TAACACGGCA 
TGCCTTACAA AGCTCTTTCC TACCCGCTCA CATACTGTTG CAGCACAGGG AGGTATCAAT 
GCTGCCCTGG GGAACATGGA AGAGGACAAC TGGAGATGGC ATTTCTATGA CACCGTGAAA 
GGCTCTGACT GGCTGGGGGA TCAGGATGCC ATCCATTACA TGACAGAGCA AGCTCCTGCC 
TCCGTGGTTG AGCTAGAAAA TTACGGTATG CCGTTTAGCA GGACTGAAGA TGGGAGGATT 
TATCAGCGTG CATTTGGTGG ACAGAGCCTC AAGTTCGGGA AAGGCGGGCA GGCCCATCGG 
TGTTGCTGTG TCGCTGATCG GACGGGCCAC TCACTCTTAC ACACCTTGTA TGGACGATCT 
CTGCGGTATG ACACCAGTTA TTTTGTGGAG TATTTCGCAC TGGATCTTCT GATGGAAAAT 
GGGGAGTGCC GTGGTGTCAT TGCACTGTGC ATAGAAGATG GGTCCATACA CCGAATAAGA 
GCAAAGAACA CTATTATTGC TACTGGGGGC TATGGGCGAA CCTACTTCAG CTGTACTTCT 
GCCCACACCA GCACAGGGGA CGGCACAGCC ATGGTCACTC GGGCTGGTTT ACCTTGCCAG 
GACTTAGAAT TTGTTCAGTT CCACCCCACA GGTATCTATG GTGCTGGCTG CCTCATCACA 
GAAGGGTGCC GTGGAGAGGG AGGCATTCTC ATCAACAGCC AAGGCGAAAG GTTCATGGAG 
AGATATGCCC CTGTTGCCAA GGACCTAGCA TCAAGAGATG TTGTGTCTCG ATCCATGACT 
CTCGAGATCC GTGAAGGAAG AGGCTGTGGC CCTGAGAAGG ATCACGTCTA CCTGCAGTTG 
CACCATCTGC CCCCTGAGCA GCTGGCCACG CGTCTGCCTG GGATCTCAGA GACGGCCATG 
ATCTTCGCCG GCGTGGATGT CACCAAGGAG CCCATTCCAG TCCTTCCCAC TGTGCATTAC 
AACATGGGCG GGATTCCCAC TAACTACAAG GGACAGGTGC TGAAGCACGT GAACGGCCAG 
GATCAGATTG TGCCTGGTCT GTACGCCTGT GGGGAGGCTG CCTGCGCCTC AGTGCATGGT 
GCCAACCGGC TTGGAGCAAA CTCTCTTTTG GACCTTGTCG TCTTTGGCCG AGCCTGTGCC 
CTGAGCATTG CAGAATCTTG CAGGCCTGGA GATAAAGTTC CTCCGATTAA GGCAAATGCT 
GGAGAAGAGT CGGTTATGAA TCTTGACAAG TTGAGATTTG CTGATGGAAG TGTAAGAACA 
TCAGAGCTGC GCCTCAGCAT GCAGAAGTCG ATGCAGAGCC ATGCCGCCGT GTTCCGTGTG 
GGAAGTGTGC TGCAAGAAGG CTGTGAAAAA GTCAGCCAGC TCTATGGAGA CCTACAGCAT 
CTGAAGACGT TTGACAGGGG AATGGTCTGG AACACAGACC TGGTGGAGAC GCTGGAGCTG 
CAGAATCTGA TGCTGTGCGC ACTGCAGACC ATATATGGTG CGGAAGCACG GAAGGAGTCA 
CGGGGAGCTC ATGCCAGGGA AGATTACAAG GTGCGGATTG ATGAGTATGA TTACTCCAAG 
CCCATCGAGG GCCAGCAGAA GAAGCCATTT GCGGAACACT GGAGGAAGCA CACCCTCTCA 
TATGTGGACA CCAAGACTGG GAAGGTTACT TTGGATTACA GACCTGTTAT TGACAAGACC 
TTGAATGAGG CTGACTGTGC TACTGTACCT CCTGCTATCC GTTCCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569112.1
CDS	42..2012
Translation	MAGVGAVSRLLRGRRLALAGATRGFHFSVGESKKASAKVSDAISTQYPVVDHEFDAVVVGAGGAGLRAAFGLSEAGFNTACLTKLFPTRSHTVAAQGGINAALGNMEEDNWRWHFYDTVKGSDWLGDQDAIHYMTEQAPASVVELENYGMPFSRTEDGRIYQRAFGGQSLKFGKGGQAHRCCCVADRTGHSLLHTLYGRSLRYDTSYFVEYFALDLLMENGECRGVIALCIEDGSIHRIRAKNTIIATGGYGRTYFSCTSAHTSTGDGTAMVTRAGLPCQDLEFVQFHPTGIYGAGCLITEGCRGEGGILINSQGERFMERYAPVAKDLASRDVVSRSMTLEIREGRGCGPEKDHVYLQLHHLPPEQLATRLPGISETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQVLKHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIAESCRPGDKVPPIKANAGEESVMNLDKLRFADGSVRTSELRLSMQKSMQSHAAVFRVGSVLQEGCEKVSQLYGDLQHLKTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVRIDEYDYSKPIEGQQKKPFAEHWRKHTLSYVDTKTGKVTLDYRPVIDKTLNEADCATVPPAIRSY

Target ORF information:

RefSeq Version	NM_130428.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus succinate dehydrogenase complex flavoprotein subunit A (Sdha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130428.1

ORF Insert Sequence:

ATGGCCGGGG TTGGCGCAGT TTCGAGACTT CTTCGCGGGC GGCGCTTGGC TCTAGCTGGG 
GCGACTCGTG GCTTTCACTT CTCTGTTGGT GAGAGCAAGA AGGCATCCGC TAAAGTTTCA 
GACGCGATTT CTACCCAGTA CCCCGTGGTG GACCATGAGT TTGATGCTGT GGTTGTAGGT 
GCAGGCGGGG CAGGCTTGCG AGCTGCATTC GGCCTTTCTG AGGCAGGCTT TAACACGGCA 
TGCCTTACAA AGCTCTTTCC TACCCGCTCA CATACTGTTG CAGCACAGGG AGGTATCAAT 
GCTGCCCTGG GGAACATGGA AGAGGACAAC TGGAGATGGC ATTTCTATGA CACCGTGAAA 
GGCTCTGACT GGCTGGGGGA TCAGGATGCC ATCCATTACA TGACAGAGCA AGCTCCTGCC 
TCCGTGGTTG AGCTAGAAAA TTACGGTATG CCGTTTAGCA GGACTGAAGA TGGGAGGATT 
TATCAGCGTG CATTTGGTGG ACAGAGCCTC AAGTTCGGGA AAGGCGGGCA GGCCCATCGG 
TGTTGCTGTG TCGCTGATCG GACGGGCCAC TCACTCTTAC ACACCTTGTA TGGACGATCT 
CTGCGGTATG ACACCAGTTA TTTTGTGGAG TATTTCGCAC TGGATCTTCT GATGGAAAAT 
GGGGAGTGCC GTGGTGTCAT TGCACTGTGC ATAGAAGATG GGTCCATACA CCGAATAAGA 
GCAAAGAACA CTATTATTGC TACTGGGGGC TATGGGCGAA CCTACTTCAG CTGTACTTCT 
GCCCACACCA GCACAGGGGA CGGCACAGCC ATGGTCACTC GGGCTGGTTT ACCTTGCCAG 
GACTTAGAAT TTGTTCAGTT CCACCCCACA GGTATCTATG GTGCTGGCTG CCTCATCACA 
GAAGGGTGCC GTGGAGAGGG AGGCATTCTC ATCAACAGCC AAGGCGAAAG GTTCATGGAG 
AGATATGCCC CTGTTGCCAA GGACCTAGCA TCAAGAGATG TTGTGTCTCG ATCCATGACT 
CTCGAGATCC GTGAAGGAAG AGGCTGTGGC CCTGAGAAGG ATCACGTCTA CCTGCAGTTG 
CACCATCTGC CCCCTGAGCA GCTGGCCACG CGTCTGCCTG GGATCTCAGA GACGGCCATG 
ATCTTCGCCG GCGTGGATGT CACCAAGGAG CCCATTCCAG TCCTTCCCAC TGTGCATTAC 
AACATGGGCG GGATTCCCAC TAACTACAAG GGACAGGTGC TGAAGCACGT GAACGGCCAG 
GATCAGATTG TGCCTGGTCT GTACGCCTGT GGGGAGGCTG CCTGCGCCTC AGTGCATGGT 
GCCAACCGGC TTGGAGCAAA CTCTCTTTTG GACCTTGTCG TCTTTGGCCG AGCCTGTGCC 
CTGAGCATTG CAGAATCTTG CAGGCCTGGA GATAAAGTTC CTCCGATTAA GGCAAATGCT 
GGAGAAGAGT CGGTTATGAA TCTTGACAAG TTGAGATTTG CTGATGGAAG TGTAAGAACA 
TCAGAGCTGC GCCTCAGCAT GCAGAAGTCG ATGCAGAGCC ATGCCGCCGT GTTCCGTGTG 
GGAAGTGTGC TGCAAGAAGG CTGTGAAAAA GTCAGCCAGC TCTATGGAGA CCTACAGCAT 
CTGAAGACGT TTGACAGGGG AATGGTCTGG AACACAGACC TGGTGGAGAC GCTGGAGCTG 
CAGAATCTGA TGCTGTGCGC ACTGCAGACC ATATATGGTG CGGAAGCACG GAAGGAGTCA 
CGGGGAGCTC ATGCCAGGGA AGATTACAAG GTGCGGATTG ATGAGTATGA TTACTCCAAG 
CCCATCGAGG GCCAGCAGAA GAAGCCATTT GCGGAACACT GGAGGAAGCA CACCCTCTCA 
TATGTGGACA CCAAGACTGG GAAGGTTACT TTGGATTACA GACCTGTTAT TGACAAGACC 
TTGAATGAGG CTGACTGTGC TACTGTACCT CCTGCTATCC GTTCCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondrial DNA Depletion in Respiratory Chain-Deficient Parkinson Disease Neurons.
Annals of neurology79(3)366-78(2016 Mar)
Gr?newald A,Rygiel KA,Hepplewhite PD,Morris CM,Picard M,Turnbull DM

Skeletal muscle Sirt3 expression and mitochondrial respiration are regulated by a prenatal low-protein diet.
The Journal of nutritional biochemistry26(2)184-9(2015 Feb)
Claycombe KJ,Roemmich JN,Johnson L,Vomhof-DeKrey EE,Johnson WT

SDHA mutations causing a multisystem mitochondrial disease: novel mutations and genetic overlap with hereditary tumors.
European journal of human genetics : EJHG23(2)202-9(2015 Feb)
Renkema GH,Wortmann SB,Smeets RJ,Venselaar H,Antoine M,Visser G,Ben-Omran T,van den Heuvel LP,Timmers HJ,Smeitink JA,Rodenburg RJ

Acute administration of l-tyrosine alters energetic metabolism of hippocampus and striatum of infant rats.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience31(5)303-7(2013 Aug)
Ramos AC,Ferreira GK,Carvalho-Silva M,Furlanetto CB,Gon?alves CL,Ferreira GC,Schuck PF,Streck EL

Intraabdominal adhesion formation is associated with differential mRNA expression of metabolic genes PDHb and SDHa.
Archives of gynecology and obstetrics286(3)683-6(2012 Sep)
Wallwiener M,Wallwiener CW,Molinas R,Rajab TK,Brucker SY,Kraemer B,Hartkopf AD

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Sdha cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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