Cacng8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cacng8
Entrez Gene ID	140729
Full Name	calcium voltage-gated channel auxiliary subunit gamma 8
General protein information	Preferred Names calcium voltage-gated channel auxiliary subunit gamma 8 Names voltage-dependent calcium channel gamma-8 subunit TARP gamma-8 calcium channel, voltage-dependent, gamma subunit 8 neuronal voltage-gated calcium channel gamma-8 subunit transmembrane AMPAR regulatory protein gamma-8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q12
Summary	Voltage-dependent calcium channels couple membrane depolarization in a number of cellular processes. These activities are regulated by distinct channels composed of the pore-forming alpha-1 subunit and the modulatory beta, alpha-2/delta and gamma subunits. The protein encoded by this gene represents one of the gamma subunits. It is an integral membrane protein that is thought to stabilize the calcium channel in an inactive (closed) state. This gene is located in a cluster with two similar gamma subunit-encoding genes on chromosome 1. The mRNA for this gene is believed to initiate translation from a non-AUG (CUG) start codon. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_080696.2	NP_542427.1	voltage-dependent calcium channel gamma-8 subunit

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cacng8	NP_542427.1
Pan troglodytes (chimpanzee)	CACNG8	XP_003953677.1
Mus musculus (house mouse)	Cacng8	NP_573453.1
Canis lupus familiaris (dog)	CACNG8	XP_005616907.1
Homo sapiens (human)	CACNG8	NP_114101.4
Danio rerio (zebrafish)	cacng8b	XP_001342922.4
Xenopus tropicalis (tropical clawed frog)	cacng8	NP_001072217.2

Gene Ontology
Bibliography

Related articles in PubMed

TARP ?-2 and ?-8 Differentially Control AMPAR Density Across Schaffer Collateral/Commissural Synapses in the Hippocampal CA1 Area.
Yamasaki M, Fukaya M, Yamazaki M, Azechi H, Natsume R, Abe M, Sakimura K, Watanabe M
The Journal of neuroscience : the official journal of the Society for Neuroscience36(15)4296-312(2016 Apr)

Activity- and development-dependent down-regulation of TARP?8 and GluA1 in cultured rat hippocampal neurons.
Wang JG, Wang YL, Xu F, Zhao JX, Zhou SY, Yu Y, Chazot PL, Wang XF, Lu CB
Acta pharmacologica Sinica37(3)303-11(2016 Mar)

TARP ?-8 glycosylation regulates the surface expression of AMPA receptors.
Zheng CY, Chang K, Suh YH, Roche KW
The Biochemical journal465(3)471-7(2015 Feb)

Patients with Dilated Cardiomyopathy and Sustained Monomorphic Ventricular Tachycardia Show Up-Regulation of KCNN3 and KCNJ2 Genes and CACNG8-Linked Left Ventricular Dysfunction.
Ortega A, Taraz?n E, Rosell?-Llet? E, Gil-Cayuela C, Lago F, Gonz?lez-Juanatey JR, Cinca J, Jorge E, Mart?nez-Dolz L, Portol?s M, Rivera M
PloS one10(12)e0145518(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AMPA-induced currents were recorded in the neurons using a whole-cell recording method. RESULTS: Four-hour exposure to AMPA significantly decreased the protein levels of TARP gamma-8 and GluA1 in the neurons at 17-18 DIV, but did not change the protein level of TARP gamma-8 in the neurons cultured at 7-8 DIV.
Title: Activity- and development-dependent down-regulation of TARP?8 and GluA1 in cultured rat hippocampal neurons.

TARP gamma-8 has an important role in the trafficking and function of the AMPA receptor complex.
Title: TARP ?-8 glycosylation regulates the surface expression of AMPA receptors.

the gamma-8 P/A-rich region is essential for binding calcineurin, suggesting that the gamma-8/calcineurin complex may regulate AMPA receptor phosphorylation and trafficking
Title: Direct association of the unique C-terminal tail of transmembrane AMPA receptor regulatory protein ?-8 with calcineurin.

The following Cacng8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacng8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13368	NM_080696.2 Latest version!	Rattus norvegicus calcium voltage-gated channel auxiliary subunit gamma 8 (Cacng8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacng8 ( NM_080696.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_542427.1 Rattus norvegicus calcium voltage-gated channel auxiliary subunit gamma 8 (Cacng8), mRNA.

Price & Availability↑

CloneID	ORa13368
Clone ID Related Accession (Same CDS sequence)	NM_080696.2
Accession Version	NM_080696.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent calcium channel gamma-8 subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF361346.1 and AC114434.4. On Sep 21, 2007 this sequence version replaced NM_080696.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF361346.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ##RefSeq-Attributes-END##

CTGGAATCAT TGAAACGCTG GAATGAAGAG AGGGGTTTGT GGTGCGAAAA GGGCGTTCAG 
GTACTACTGA CCACCATAGG CGCCTTTGCA GCTTTTGGCC TCATGACCAT CGCCATCAGC 
ACTGACTACT GGCTCTACAC AAGAGCTCTC ATCTGCAACA CCACCAACCT CACAGCAGGT 
GATGATGGAC CACCCCATCG TGGGGGCAGT GGCTCCTCAG AGAAGAAGGA CCCTGGGGGC 
CTCACACATT CAGGCCTCTG GCGGATATGC TGCCTGGAAG GGTTGAAAAG AGGTGTCTGC 
GTGAAGATCA ACCACTTCCC GGAGGACACG GACTACGACC ACGACAGCGC GGAGTACCTG 
CTCCGAGTAG TCCGGGCCTC CAGCATCTTT CCTATCCTGA GCGCCATCCT GCTGCTGCTC 
GGGGGCGTGT GCGTAGCTGC CTCTCGCGTC TACAAATCCA AAAGGAACAT CATCCTGGGC 
GCAGGGATCC TGTTCGTGGC AGCAGGCCTG AGCAACATCA TCGGGGTGAT CGTGTACATA 
TCGGCAAACG CGGGCGAGCC AGGCCCCAAG AGGGACGAGG AGAAGAAAAA CCACTATTCG 
TATGGCTGGT CCTTCTACTT CGGCGGGCTG TCATTCATCC TGGCCGAGGT GATCGGCGTG 
CTAGCCGTCA ACATCTACAT CGAGCGCAGC CGCGAGGCAC ACTGCCAATC ACGCTCGGAC 
CTACTCAAGG CCGGCGGCGG CGCGGGCGGC AGTGGCGGGA GCGGCCCCTC GGCCATCCTC 
CGTCTGCCCA GTTACCGCTT CCGCTACCGC CGCCGCTCCC GCTCCAGCTC CCGAGGCTCC 
AGCGAGGCCT CGCCATCGCG GGATGCGTCT CCCGGCGGCC CCGGGGGCCC GGGCTTCGCC 
TCCACGGACA TCTCCATGTA CACGCTCAGT CGCGACCCGT CCAAGGGCAG CGTGGCTGCG 
GGGCTGGCGA GCGCCGGGGG TGGAGGCGGC GGTGCCGGCG TGGGTGCCTA CGGCGGGGCG 
GCCGGGGCAG CGGGGGGCGG CGGGACGGGC TCGGAGCGGG ACCGAGGGAG CTCAGCGGGC 
TTCCTCACGC TGCACAACGC CTTCCCCAAG GAGGCGGCGT CCGGCGTCAC GGTCACGGTC 
ACCGGACCGC CCGCTGCGCC GGCGCCCGCG CCGCCCGCTC CTGCAGCGCC CGCGCCCGGG 
ACGCTGTCCA AAGAGGCCGC CGCGTCCAAC ACCAACACGC TCAACAGGAA AACCACGCCC 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542427.1
CDS	1..1266
Translation	LESLKRWNEERGLWCEKGVQVLLTTIGAFAAFGLMTIAISTDYWLYTRALICNTTNLTAGDDGPPHRGGSGSSEKKDPGGLTHSGLWRICCLEGLKRGVCVKINHFPEDTDYDHDSAEYLLRVVRASSIFPILSAILLLLGGVCVAASRVYKSKRNIILGAGILFVAAGLSNIIGVIVYISANAGEPGPKRDEEKKNHYSYGWSFYFGGLSFILAEVIGVLAVNIYIERSREAHCQSRSDLLKAGGGAGGSGGSGPSAILRLPSYRFRYRRRSRSSSRGSSEASPSRDASPGGPGGPGFASTDISMYTLSRDPSKGSVAAGLASAGGGGGGAGVGAYGGAAGAAGGGGTGSERDRGSSAGFLTLHNAFPKEAASGVTVTVTGPPAAPAPAPPAPAAPAPGTLSKEAAASNTNTLNRKTTPV

Target ORF information:

RefSeq Version	NM_080696.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel auxiliary subunit gamma 8 (Cacng8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080696.2

ORF Insert Sequence:

CTGGAATCAT TGAAACGCTG GAATGAAGAG AGGGGTTTGT GGTGCGAAAA GGGCGTTCAG 
GTACTACTGA CCACCATAGG CGCCTTTGCA GCTTTTGGCC TCATGACCAT CGCCATCAGC 
ACTGACTACT GGCTCTACAC AAGAGCTCTC ATCTGCAACA CCACCAACCT CACAGCAGGT 
GATGATGGAC CACCCCATCG TGGGGGCAGT GGCTCCTCAG AGAAGAAGGA CCCTGGGGGC 
CTCACACATT CAGGCCTCTG GCGGATATGC TGCCTGGAAG GGTTGAAAAG AGGTGTCTGC 
GTGAAGATCA ACCACTTCCC GGAGGACACG GACTACGACC ACGACAGCGC GGAGTACCTG 
CTCCGAGTAG TCCGGGCCTC CAGCATCTTT CCTATCCTGA GCGCCATCCT GCTGCTGCTC 
GGGGGCGTGT GCGTAGCTGC CTCTCGCGTC TACAAATCCA AAAGGAACAT CATCCTGGGC 
GCAGGGATCC TGTTCGTGGC AGCAGGCCTG AGCAACATCA TCGGGGTGAT CGTGTACATA 
TCGGCAAACG CGGGCGAGCC AGGCCCCAAG AGGGACGAGG AGAAGAAAAA CCACTATTCG 
TATGGCTGGT CCTTCTACTT CGGCGGGCTG TCATTCATCC TGGCCGAGGT GATCGGCGTG 
CTAGCCGTCA ACATCTACAT CGAGCGCAGC CGCGAGGCAC ACTGCCAATC ACGCTCGGAC 
CTACTCAAGG CCGGCGGCGG CGCGGGCGGC AGTGGCGGGA GCGGCCCCTC GGCCATCCTC 
CGTCTGCCCA GTTACCGCTT CCGCTACCGC CGCCGCTCCC GCTCCAGCTC CCGAGGCTCC 
AGCGAGGCCT CGCCATCGCG GGATGCGTCT CCCGGCGGCC CCGGGGGCCC GGGCTTCGCC 
TCCACGGACA TCTCCATGTA CACGCTCAGT CGCGACCCGT CCAAGGGCAG CGTGGCTGCG 
GGGCTGGCGA GCGCCGGGGG TGGAGGCGGC GGTGCCGGCG TGGGTGCCTA CGGCGGGGCG 
GCCGGGGCAG CGGGGGGCGG CGGGACGGGC TCGGAGCGGG ACCGAGGGAG CTCAGCGGGC 
TTCCTCACGC TGCACAACGC CTTCCCCAAG GAGGCGGCGT CCGGCGTCAC GGTCACGGTC 
ACCGGACCGC CCGCTGCGCC GGCGCCCGCG CCGCCCGCTC CTGCAGCGCC CGCGCCCGGG 
ACGCTGTCCA AAGAGGCCGC CGCGTCCAAC ACCAACACGC TCAACAGGAA AACCACGCCC 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TARP ?-2 and ?-8 Differentially Control AMPAR Density Across Schaffer Collateral/Commissural Synapses in the Hippocampal CA1 Area.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(15)4296-312(2016 Apr)
Yamasaki M,Fukaya M,Yamazaki M,Azechi H,Natsume R,Abe M,Sakimura K,Watanabe M

Activity- and development-dependent down-regulation of TARP?8 and GluA1 in cultured rat hippocampal neurons.
Acta pharmacologica Sinica37(3)303-11(2016 Mar)
Wang JG,Wang YL,Xu F,Zhao JX,Zhou SY,Yu Y,Chazot PL,Wang XF,Lu CB

TARP ?-8 glycosylation regulates the surface expression of AMPA receptors.
The Biochemical journal465(3)471-7(2015 Feb)
Zheng CY,Chang K,Suh YH,Roche KW

Patients with Dilated Cardiomyopathy and Sustained Monomorphic Ventricular Tachycardia Show Up-Regulation of KCNN3 and KCNJ2 Genes and CACNG8-Linked Left Ventricular Dysfunction.
PloS one10(12)e0145518(2015)
Ortega A,Taraz?n E,Rosell?-Llet? E,Gil-Cayuela C,Lago F,Gonz?lez-Juanatey JR,Cinca J,Jorge E,Mart?nez-Dolz L,Portol?s M,Rivera M

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Cacng8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報