Rab3d cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rab3d
Entrez Gene ID	140665
Full Name	RAB3D, member RAS oncogene family
General protein information	Preferred Names RAB3D, member RAS oncogene family Names GTP-binding protein Rab-3D
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q13
Summary	may regulate membrane fusion in Fc epsilonRI-stimulated mast cell exocytosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017595422.1	XP_017450911.1	GTP-binding protein Rab-3D isoform X2
XM_006242593.1	XP_006242655.1	GTP-binding protein Rab-3D isoform X1
NM_080580.2	NP_542147.1	GTP-binding protein Rab-3D
XM_006242594.3	XP_006242656.1	GTP-binding protein Rab-3D isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	rab3d	NP_001005621.1
Mus musculus (house mouse)	Rab3d	NP_114080.2
Danio rerio (zebrafish)	rab3da	NP_001139069.1
Homo sapiens (human)	RAB3D	NP_004274.1
Canis lupus familiaris (dog)	RAB3D	XP_533913.1
Bos taurus (cattle)	RAB3D	NP_001178304.1
Pan troglodytes (chimpanzee)	RAB3D	XP_001167816.1
Rattus norvegicus (Norway rat)	Rab3d	NP_542147.1
	RAB3D	XP_001104198.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification and characterization of multiple novel Rab-myosin Va interactions.
Lindsay AJ, Jollivet F, Horgan CP, Khan AR, Raposo G, McCaffrey MW, Goud B
Molecular biology of the cell24(21)3420-34(2013 Nov)

A complete Rab screening reveals novel insights in Weibel-Palade body exocytosis.
Zografou S, Basagiannis D, Papafotika A, Shirakawa R, Horiuchi H, Auerbach D, Fukuda M, Christoforidis S
Journal of cell science125(Pt 20)4780-90(2012 Oct)

Rab3D regulates amylase levels, not agonist-induced amylase release, in AR42J cells.
Limi S, Ojakian G, Raffaniello R
Cellular & molecular biology letters17(2)258-73(2012 Jun)

Tctex-1, a novel interaction partner of Rab3D, is required for osteoclastic bone resorption.
Pavlos NJ, Cheng TS, Qin A, Ng PY, Feng HT, Ang ES, Carrello A, Sung CH, Jahn R, Zheng MH, Xu J
Molecular and cellular biology31(7)1551-64(2011 Apr)

Roles of myosin Va and Rab3D in membrane remodeling of immature secretory granules.
K?gel T, Gerdes HH
Cellular and molecular neurobiology30(8)1303-8(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rab3D is not involved in agonist-induced exocytosis in AR42J cells. Instead, Rab3D may regulate amylase content in these cells.
Title: Rab3D regulates amylase levels, not agonist-induced amylase release, in AR42J cells.

These data suggest an implication of myosin Va and Rab3D in the maturation of SGs where they participate in overlapping but not identical tasks.
Title: Roles of myosin Va and Rab3D in membrane remodeling of immature secretory granules.

the present data suggest that Rab3D and rabphilin may regulate the secretory pathway at a much earlier stage than what has hitherto been assumed.
Title: Novel localization of Rab3D in rat intestinal goblet cells and Brunner's gland acinar cells suggests a role in early Golgi trafficking.

In parotid acini, Rab3D doesn't dissociate from cell membranes or redistribute to the plasma membrane during secretion, and may play an inhibitory role in regulated secretion.
Title: Rab3D redistribution and function in rat parotid acini.

pIgA-R traffics through postendosomal rab3D-, rab11a-, and syntaxin 2-associated compartments, implicating these proteins in the regulation of transcytosis.
Title: Intracellular accumulation of pIgA-R and regulators of transcytotic trafficking in cholestatic rat hepatocytes.

The following Rab3d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rab3d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11808	NM_080580.2 Latest version!	Rattus norvegicus RAB3D, member RAS oncogene family (Rab3d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa11808	XM_017595422.1 Latest version!	Rattus norvegicus RAB3D, member RAS oncogene family (Rab3d), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa11808	XM_006242594.3 Latest version!	Rattus norvegicus RAB3D, member RAS oncogene family (Rab3d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa30876	XM_006242593.1 Latest version!	Rattus norvegicus RAB3D, member RAS oncogene family (Rab3d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rab3d ( NM_080580.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_542147.1 Rattus norvegicus RAB3D, member RAS oncogene family (Rab3d), mRNA.

Price & Availability↑

CloneID	ORa11808
Clone ID Related Accession (Same CDS sequence)	XM_017595422.1 , NM_080580.2 , XM_006242594.3
Accession Version	NM_080580.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	GTP-binding protein Rab-3D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081741.1. On Oct 17, 2004 this sequence version replaced NM_080580.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081741.1, FQ075728.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCCG CTAGTGAGCC CCCTGCCAGC CCACGGGACG CTGCCGACCA GAACTTTGAC 
TACATGTTCA AACTGCTTTT GATCGGGAAC AGCAGCGTAG GCAAGACCTC CTTCCTGTTC 
CGCTATGCCG ATGACTCCTT CACCCCCGCC TTCGTGAGCA CAGTGGGCAT TGACTTCAAG 
GTCAAGACAG TCTACAGACA TGACAAGAGG ATCAAGCTGC AGATCTGGGA CACAGCAGGC 
CAGGAGCGCT ACCGGACAAT CACCACGGCC TACTACCGTG GAGCCATGGG CTTCCTGCTG 
ATGTATGACA TCGCCAACCA GGAGTCCTTC ACAGCTGTGC AGGACTGGGC TACGCAGATT 
AAAACCTATT CCTGGGACAA CGCCCAGGTC ATCCTTGTGG GGAACAAGTG TGACCTGGAG 
GATGAACGGG TCGTATCTGC TGAGGATGGC CAGAGGCTCG CTGGTGATCT TGGTTTTGAG 
TTCTTCGAGG CCAGCGCCAA GGAGAACATC AACGTGAAGC AGGTGTTCGA GCGCCTGGTG 
GATATCATCT GTGACAAGAT GAATGAGTCC CTGGAACCCA GCTCCAGCCC AGGCAGCAAT 
GGGAAAGGCC CAGCCCTCGG GGATACCCCA CCCCCTCAGC CGAGCAGCTG CGGCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542147.1
CDS	110..769
Translation	MASASEPPASPRDAADQNFDYMFKLLLIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAGQERYRTITTAYYRGAMGFLLMYDIANQESFTAVQDWATQIKTYSWDNAQVILVGNKCDLEDERVVSAEDGQRLAGDLGFEFFEASAKENINVKQVFERLVDIICDKMNESLEPSSSPGSNGKGPALGDTPPPQPSSCGC

Target ORF information:

RefSeq Version	NM_080580.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAB3D, member RAS oncogene family (Rab3d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080580.2

ORF Insert Sequence:

ATGGCATCCG CTAGTGAGCC CCCTGCCAGC CCACGGGACG CTGCCGACCA GAACTTTGAC 
TACATGTTCA AACTGCTTTT GATCGGGAAC AGCAGCGTAG GCAAGACCTC CTTCCTGTTC 
CGCTATGCCG ATGACTCCTT CACCCCCGCC TTCGTGAGCA CAGTGGGCAT TGACTTCAAG 
GTCAAGACAG TCTACAGACA TGACAAGAGG ATCAAGCTGC AGATCTGGGA CACAGCAGGC 
CAGGAGCGCT ACCGGACAAT CACCACGGCC TACTACCGTG GAGCCATGGG CTTCCTGCTG 
ATGTATGACA TCGCCAACCA GGAGTCCTTC ACAGCTGTGC AGGACTGGGC TACGCAGATT 
AAAACCTATT CCTGGGACAA CGCCCAGGTC ATCCTTGTGG GGAACAAGTG TGACCTGGAG 
GATGAACGGG TCGTATCTGC TGAGGATGGC CAGAGGCTCG CTGGTGATCT TGGTTTTGAG 
TTCTTCGAGG CCAGCGCCAA GGAGAACATC AACGTGAAGC AGGTGTTCGA GCGCCTGGTG 
GATATCATCT GTGACAAGAT GAATGAGTCC CTGGAACCCA GCTCCAGCCC AGGCAGCAAT 
GGGAAAGGCC CAGCCCTCGG GGATACCCCA CCCCCTCAGC CGAGCAGCTG CGGCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rab3d ( XM_017595422.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450911.1 Rattus norvegicus RAB3D, member RAS oncogene family (Rab3d), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa11808
Clone ID Related Accession (Same CDS sequence)	XM_017595422.1 , NM_080580.2 , XM_006242594.3
Accession Version	XM_017595422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	GTP-binding protein Rab-3D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCCG CTAGTGAGCC CCCTGCCAGC CCACGGGACG CTGCCGACCA GAACTTTGAC 
TACATGTTCA AACTGCTTTT GATCGGGAAC AGCAGCGTAG GCAAGACCTC CTTCCTGTTC 
CGCTATGCCG ATGACTCCTT CACCCCCGCC TTCGTGAGCA CAGTGGGCAT TGACTTCAAG 
GTCAAGACAG TCTACAGACA TGACAAGAGG ATCAAGCTGC AGATCTGGGA CACAGCAGGC 
CAGGAGCGCT ACCGGACAAT CACCACGGCC TACTACCGTG GAGCCATGGG CTTCCTGCTG 
ATGTATGACA TCGCCAACCA GGAGTCCTTC ACAGCTGTGC AGGACTGGGC TACGCAGATT 
AAAACCTATT CCTGGGACAA CGCCCAGGTC ATCCTTGTGG GGAACAAGTG TGACCTGGAG 
GATGAACGGG TCGTATCTGC TGAGGATGGC CAGAGGCTCG CTGGTGATCT TGGTTTTGAG 
TTCTTCGAGG CCAGCGCCAA GGAGAACATC AACGTGAAGC AGGTGTTCGA GCGCCTGGTG 
GATATCATCT GTGACAAGAT GAATGAGTCC CTGGAACCCA GCTCCAGCCC AGGCAGCAAT 
GGGAAAGGCC CAGCCCTCGG GGATACCCCA CCCCCTCAGC CGAGCAGCTG CGGCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450911.1
CDS	118..777
Translation	MASASEPPASPRDAADQNFDYMFKLLLIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAGQERYRTITTAYYRGAMGFLLMYDIANQESFTAVQDWATQIKTYSWDNAQVILVGNKCDLEDERVVSAEDGQRLAGDLGFEFFEASAKENINVKQVFERLVDIICDKMNESLEPSSSPGSNGKGPALGDTPPPQPSSCGC

Target ORF information:

RefSeq Version	XM_017595422.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAB3D, member RAS oncogene family (Rab3d), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595422.1

ORF Insert Sequence:

ATGGCATCCG CTAGTGAGCC CCCTGCCAGC CCACGGGACG CTGCCGACCA GAACTTTGAC 
TACATGTTCA AACTGCTTTT GATCGGGAAC AGCAGCGTAG GCAAGACCTC CTTCCTGTTC 
CGCTATGCCG ATGACTCCTT CACCCCCGCC TTCGTGAGCA CAGTGGGCAT TGACTTCAAG 
GTCAAGACAG TCTACAGACA TGACAAGAGG ATCAAGCTGC AGATCTGGGA CACAGCAGGC 
CAGGAGCGCT ACCGGACAAT CACCACGGCC TACTACCGTG GAGCCATGGG CTTCCTGCTG 
ATGTATGACA TCGCCAACCA GGAGTCCTTC ACAGCTGTGC AGGACTGGGC TACGCAGATT 
AAAACCTATT CCTGGGACAA CGCCCAGGTC ATCCTTGTGG GGAACAAGTG TGACCTGGAG 
GATGAACGGG TCGTATCTGC TGAGGATGGC CAGAGGCTCG CTGGTGATCT TGGTTTTGAG 
TTCTTCGAGG CCAGCGCCAA GGAGAACATC AACGTGAAGC AGGTGTTCGA GCGCCTGGTG 
GATATCATCT GTGACAAGAT GAATGAGTCC CTGGAACCCA GCTCCAGCCC AGGCAGCAAT 
GGGAAAGGCC CAGCCCTCGG GGATACCCCA CCCCCTCAGC CGAGCAGCTG CGGCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rab3d ( XM_006242594.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242656.1 Rattus norvegicus RAB3D, member RAS oncogene family (Rab3d), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa11808
Clone ID Related Accession (Same CDS sequence)	XM_017595422.1 , NM_080580.2 , XM_006242594.3
Accession Version	XM_006242594.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	GTP-binding protein Rab-3D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242594.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCCG CTAGTGAGCC CCCTGCCAGC CCACGGGACG CTGCCGACCA GAACTTTGAC 
TACATGTTCA AACTGCTTTT GATCGGGAAC AGCAGCGTAG GCAAGACCTC CTTCCTGTTC 
CGCTATGCCG ATGACTCCTT CACCCCCGCC TTCGTGAGCA CAGTGGGCAT TGACTTCAAG 
GTCAAGACAG TCTACAGACA TGACAAGAGG ATCAAGCTGC AGATCTGGGA CACAGCAGGC 
CAGGAGCGCT ACCGGACAAT CACCACGGCC TACTACCGTG GAGCCATGGG CTTCCTGCTG 
ATGTATGACA TCGCCAACCA GGAGTCCTTC ACAGCTGTGC AGGACTGGGC TACGCAGATT 
AAAACCTATT CCTGGGACAA CGCCCAGGTC ATCCTTGTGG GGAACAAGTG TGACCTGGAG 
GATGAACGGG TCGTATCTGC TGAGGATGGC CAGAGGCTCG CTGGTGATCT TGGTTTTGAG 
TTCTTCGAGG CCAGCGCCAA GGAGAACATC AACGTGAAGC AGGTGTTCGA GCGCCTGGTG 
GATATCATCT GTGACAAGAT GAATGAGTCC CTGGAACCCA GCTCCAGCCC AGGCAGCAAT 
GGGAAAGGCC CAGCCCTCGG GGATACCCCA CCCCCTCAGC CGAGCAGCTG CGGCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242656.1
CDS	233..892
Translation	MASASEPPASPRDAADQNFDYMFKLLLIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAGQERYRTITTAYYRGAMGFLLMYDIANQESFTAVQDWATQIKTYSWDNAQVILVGNKCDLEDERVVSAEDGQRLAGDLGFEFFEASAKENINVKQVFERLVDIICDKMNESLEPSSSPGSNGKGPALGDTPPPQPSSCGC

Target ORF information:

RefSeq Version	XM_006242594.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAB3D, member RAS oncogene family (Rab3d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242594.3

ORF Insert Sequence:

ATGGCATCCG CTAGTGAGCC CCCTGCCAGC CCACGGGACG CTGCCGACCA GAACTTTGAC 
TACATGTTCA AACTGCTTTT GATCGGGAAC AGCAGCGTAG GCAAGACCTC CTTCCTGTTC 
CGCTATGCCG ATGACTCCTT CACCCCCGCC TTCGTGAGCA CAGTGGGCAT TGACTTCAAG 
GTCAAGACAG TCTACAGACA TGACAAGAGG ATCAAGCTGC AGATCTGGGA CACAGCAGGC 
CAGGAGCGCT ACCGGACAAT CACCACGGCC TACTACCGTG GAGCCATGGG CTTCCTGCTG 
ATGTATGACA TCGCCAACCA GGAGTCCTTC ACAGCTGTGC AGGACTGGGC TACGCAGATT 
AAAACCTATT CCTGGGACAA CGCCCAGGTC ATCCTTGTGG GGAACAAGTG TGACCTGGAG 
GATGAACGGG TCGTATCTGC TGAGGATGGC CAGAGGCTCG CTGGTGATCT TGGTTTTGAG 
TTCTTCGAGG CCAGCGCCAA GGAGAACATC AACGTGAAGC AGGTGTTCGA GCGCCTGGTG 
GATATCATCT GTGACAAGAT GAATGAGTCC CTGGAACCCA GCTCCAGCCC AGGCAGCAAT 
GGGAAAGGCC CAGCCCTCGG GGATACCCCA CCCCCTCAGC CGAGCAGCTG CGGCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rab3d ( XM_006242593.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242655.1 Rattus norvegicus RAB3D, member RAS oncogene family (Rab3d), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30876
Clone ID Related Accession (Same CDS sequence)	XM_006242593.1
Accession Version	XM_006242593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	GTP-binding protein Rab-3D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGGGC TGAACTGGGG TCCGCGCGAG CCTGGGTCCC ACACCCCCAA TCCCACCAAG 
ATGGCATCCG CTAGTGAGCC CCCTGCCAGC CCACGGGACG CTGCCGACCA GAACTTTGAC 
TACATGTTCA AACTGCTTTT GATCGGGAAC AGCAGCGTAG GCAAGACCTC CTTCCTGTTC 
CGCTATGCCG ATGACTCCTT CACCCCCGCC TTCGTGAGCA CAGTGGGCAT TGACTTCAAG 
GTCAAGACAG TCTACAGACA TGACAAGAGG ATCAAGCTGC AGATCTGGGA CACAGCAGGC 
CAGGAGCGCT ACCGGACAAT CACCACGGCC TACTACCGTG GAGCCATGGG CTTCCTGCTG 
ATGTATGACA TCGCCAACCA GGAGTCCTTC ACAGCTGTGC AGGACTGGGC TACGCAGATT 
AAAACCTATT CCTGGGACAA CGCCCAGGTC ATCCTTGTGG GGAACAAGTG TGACCTGGAG 
GATGAACGGG TCGTATCTGC TGAGGATGGC CAGAGGCTCG CTGGTGATCT TGGTTTTGAG 
TTCTTCGAGG CCAGCGCCAA GGAGAACATC AACGTGAAGC AGGTGTTCGA GCGCCTGGTG 
GATATCATCT GTGACAAGAT GAATGAGTCC CTGGAACCCA GCTCCAGCCC AGGCAGCAAT 
GGGAAAGGCC CAGCCCTCGG GGATACCCCA CCCCCTCAGC CGAGCAGCTG CGGCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242655.1
CDS	46..765
Translation	MTGLNWGPREPGSHTPNPTKMASASEPPASPRDAADQNFDYMFKLLLIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAGQERYRTITTAYYRGAMGFLLMYDIANQESFTAVQDWATQIKTYSWDNAQVILVGNKCDLEDERVVSAEDGQRLAGDLGFEFFEASAKENINVKQVFERLVDIICDKMNESLEPSSSPGSNGKGPALGDTPPPQPSSCGC

Target ORF information:

RefSeq Version	XM_006242593.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAB3D, member RAS oncogene family (Rab3d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242593.1

ORF Insert Sequence:

ATGACAGGGC TGAACTGGGG TCCGCGCGAG CCTGGGTCCC ACACCCCCAA TCCCACCAAG 
ATGGCATCCG CTAGTGAGCC CCCTGCCAGC CCACGGGACG CTGCCGACCA GAACTTTGAC 
TACATGTTCA AACTGCTTTT GATCGGGAAC AGCAGCGTAG GCAAGACCTC CTTCCTGTTC 
CGCTATGCCG ATGACTCCTT CACCCCCGCC TTCGTGAGCA CAGTGGGCAT TGACTTCAAG 
GTCAAGACAG TCTACAGACA TGACAAGAGG ATCAAGCTGC AGATCTGGGA CACAGCAGGC 
CAGGAGCGCT ACCGGACAAT CACCACGGCC TACTACCGTG GAGCCATGGG CTTCCTGCTG 
ATGTATGACA TCGCCAACCA GGAGTCCTTC ACAGCTGTGC AGGACTGGGC TACGCAGATT 
AAAACCTATT CCTGGGACAA CGCCCAGGTC ATCCTTGTGG GGAACAAGTG TGACCTGGAG 
GATGAACGGG TCGTATCTGC TGAGGATGGC CAGAGGCTCG CTGGTGATCT TGGTTTTGAG 
TTCTTCGAGG CCAGCGCCAA GGAGAACATC AACGTGAAGC AGGTGTTCGA GCGCCTGGTG 
GATATCATCT GTGACAAGAT GAATGAGTCC CTGGAACCCA GCTCCAGCCC AGGCAGCAAT 
GGGAAAGGCC CAGCCCTCGG GGATACCCCA CCCCCTCAGC CGAGCAGCTG CGGCTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification and characterization of multiple novel Rab-myosin Va interactions.
Molecular biology of the cell24(21)3420-34(2013 Nov)
Lindsay AJ,Jollivet F,Horgan CP,Khan AR,Raposo G,McCaffrey MW,Goud B

A complete Rab screening reveals novel insights in Weibel-Palade body exocytosis.
Journal of cell science125(Pt 20)4780-90(2012 Oct)
Zografou S,Basagiannis D,Papafotika A,Shirakawa R,Horiuchi H,Auerbach D,Fukuda M,Christoforidis S

Rab3D regulates amylase levels, not agonist-induced amylase release, in AR42J cells.
Cellular & molecular biology letters17(2)258-73(2012 Jun)
Limi S,Ojakian G,Raffaniello R

Tctex-1, a novel interaction partner of Rab3D, is required for osteoclastic bone resorption.
Molecular and cellular biology31(7)1551-64(2011 Apr)
Pavlos NJ,Cheng TS,Qin A,Ng PY,Feng HT,Ang ES,Carrello A,Sung CH,Jahn R,Zheng MH,Xu J

Roles of myosin Va and Rab3D in membrane remodeling of immature secretory granules.
Cellular and molecular neurobiology30(8)1303-8(2010 Nov)
K?gel T,Gerdes HH

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Rab3d cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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