Chek1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chek1
Entrez Gene ID	140583
Full Name	checkpoint kinase 1
General protein information	Preferred Names checkpoint kinase 1 Names serine/threonine-protein kinase Chk1 CHK1 checkpoint homolog checkpoint kinase 1 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q21
Summary	protein kinase that can bind to single-stranded DNA; may function in DNA replication and repair [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_080400.1	NP_536325.1	serine/threonine-protein kinase Chk1
XM_008766042.2	XP_008764264.1	serine/threonine-protein kinase Chk1 isoform X1
XM_008766043.1	XP_008764265.1	serine/threonine-protein kinase Chk1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CHEK1	NP_001091492.1
Gallus gallus (chicken)	STT3A	NP_989676.1
Saccharomyces cerevisiae (baker's yeast)	CHK1	NP_009833.1
Eremothecium gossypii	AGOS_AEL185C	NP_984676.1
Magnaporthe oryzae (rice blast fungus)	MGG_03729	XP_003716151.1
Xenopus tropicalis (tropical clawed frog)	chek1	XP_002937561.2
Homo sapiens (human)	CHEK1	NP_001265.2
Rattus norvegicus (Norway rat)	Chek1	NP_536325.1
Caenorhabditis elegans (roundworm)	chk-1	NP_001024233.1
Kluyveromyces lactis	KLLA0F01276g	XP_455137.1
Schizosaccharomyces pombe (fission yeast)	chk1	NP_588070.1
	LOC713358	XP_001111431.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008118	XP_317338.4
Pan troglodytes (chimpanzee)	CHEK1	XP_001146296.1
Mus musculus (house mouse)	Chek1	NP_031717.2
Neurospora crassa	NCU08346	XP_965249.2
Canis lupus familiaris (dog)	CHEK1	XP_852191.1
Danio rerio (zebrafish)	chek1	NP_956487.1
Drosophila melanogaster (fruit fly)	grp	NP_723986.1

Gene Ontology
Bibliography

Related articles in PubMed

hHR23A is required to control the basal turnover of Chk1.
Tan X, Liang RY, Chuang SM
Cellular signalling27(11)2304-13(2015 Nov)

Regulated degradation of Chk1 by chaperone-mediated autophagy in response to DNA damage.
Park C, Suh Y, Cuervo AM
Nature communications66823(2015 Apr)

A bioinformatics approach identifies signal transducer and activator of transcription-3 and checkpoint kinase 1 as upstream regulators of kidney injury molecule-1 after kidney injury.
Ajay AK, Kim TM, Ramirez-Gonzalez V, Park PJ, Frank DA, Vaidya VS
Journal of the American Society of Nephrology : JASN25(1)105-18(2014 Jan)

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma na?ve to therapy.
Pieragostino D, Agnifili L, Fasanella V, D'Aguanno S, Mastropasqua R, Di Ilio C, Sacchetta P, Urbani A, Del Boccio P
Molecular bioSystems9(6)1108-16(2013 Jun)

CK1? kinase activity is modulated by Chk1-mediated phosphorylation.
Bischof J, Randoll SJ, S??ner N, Henne-Bruns D, Pinna LA, Knippschild U
PloS one8(7)e68803(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Chaperone-mediated autophagy, in response to genotoxic stress, is regulated by degradation of activated Chk1.
Title: Regulated degradation of Chk1 by chaperone-mediated autophagy in response to DNA damage.

KIM-1 expression increased significantly in a manner that corresponded temporally and regionally with increased phosphorylation of checkpoint kinase 1 (Chk1) and STAT3 in acute kidney injury.
Title: A bioinformatics approach identifies signal transducer and activator of transcription-3 and checkpoint kinase 1 as upstream regulators of kidney injury molecule-1 after kidney injury.

Chk1 plays a role in regulating RALT protein stability by facilitating the interaction between 14-3-3 and RALT.
Title: Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3.

Casein kinase 1 delta activity is modulated by Chk1-mediated phosphorylation.
Title: CK1? kinase activity is modulated by Chk1-mediated phosphorylation.

Chk1 is essential for neuronal survival, and perturbation of this pathway increases a cell's sensitivity to naturally accumulating DNA damage.
Title: Chk1 has an essential role in the survival of differentiated cortical neurons in the absence of DNA damage.

The following Chek1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chek1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12040	NM_080400.1 Latest version!	Rattus norvegicus checkpoint kinase 1 (Chek1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa44166	XM_008766042.2 Latest version!	Rattus norvegicus checkpoint kinase 1 (Chek1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa44166	XM_008766043.1 Latest version!	Rattus norvegicus checkpoint kinase 1 (Chek1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chek1 ( NM_080400.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_536325.1 Rattus norvegicus checkpoint kinase 1 (Chek1), mRNA.

Price & Availability↑

CloneID	ORa12040
Clone ID Related Accession (Same CDS sequence)	NM_080400.1
Accession Version	NM_080400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase Chk1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF414135.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF414135.1, AF443592.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGTGC CTTTTGTGGA AGACTGGGAT TTGGTGCAAA CTTTGGGAGA AGGCGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAATA ACTGAAGAAG CTGTTGCAGT GAAAATTGTA 
GACATGAAGC GGGCCATAGA CTGCCCAGAA AATATTAAGA AAGAGATCTG CATCAATAAG 
ATGTTAAATC ATGAGAATGT AGTGAAATTC TATGGCCACA GGAGGGAAGG CAACATCCAG 
TACCTGTTTC TGGAGTACTG TAGTGGAGGA GAACTCTTTG ATAGAATAGA GCCAGACATA 
GGGATGCCTG AACAAGATGC TCAGAGGTTT TTCCATCAGC TCATGGCGGG GGTGGTTTAT 
CTTCATGGCA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAACCTCCT CTTGGATGAA 
AGGGATAATC TCAAAATCTC TGACTTTGGC TTGGCAACAG TGTTTCGGCA TAATAATCGT 
GAGCGCTTAC TGAACAAGAT GTGTGGGACT TTACCTTATG TCGCTCCAGA GCTTCTAAAG 
AGGAAAGAAT TTCACGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT GCTTACTGCA 
ATGTTGGCTG GAGAATTGCC TTGGGACCAG CCCAGTGATA GCTGTCAAGA ATATTCTGAT 
TGGAAAGAAA AGAAAACCTA TCTCAATCCT TGGAAAAAAA TTGATTCTGC TCCTCTGGCT 
TTGCTTCATA AAATTCTAGT TGAGAATCCA TCAGCAAGGA TCACCATCCC AGACATTAAA 
AAAGATAGAT GGTATAACAA ACCACTTAAC AGAGGAGCGA AGAGGCCACG TGCTACTTCA 
GGTGGTATGT CAGAGTCTTC TAGTGGATTC TCTAAGCACA TTCATTCCAA TTTGGACTTT 
TCTCCAATAA ATAGTGGTTC CAGTGAAGAA AACGTGAAGT TCTCTAGTTC CCAGCCAGAG 
CCTCGGACAG GGCTTTCCTT GTGGGACACT GGCCCCTCAA ATGTGGACAA ACTGGTGCAA 
GGCATCAGTT TTTCCCAGCC TACGTGTCCT GATCATATGC TTGTAAACAG TCAGCTACTT 
GGTACCCCTG GATCTTCACA GAACCCCTGG CAGCGCTTGG TCAAAAGGAT GACACGATTC 
TTCACTAAAT TGGATGCAGA CAAATCTTAC CAATGCCTGA AAGAGACCTT TGAGAAGTTG 
GGCTATCAGT GGAAGAAGAG TTGTATGAAT CAGGTTACTG TATCAACAAT GGATAGAAGA 
AACAATAAGT TGATTTTCAA AATAAATTTG GTAGAAATGG ATGAGAAGAT ACTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGGTTAGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTCAGCG ATATCGTGAG CAGCCAGAAG GTTTGGTTCC CTGTTACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536325.1
CDS	46..1476
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRITEEAVAVKIVDMKRAIDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEQDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRHNNRERLLNKMCGTLPYVAPELLKRKEFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLNRGAKRPRATSGGMSESSSGFSKHIHSNLDFSPINSGSSEENVKFSSSQPEPRTGLSLWDTGPSNVDKLVQGISFSQPTCPDHMLVNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETFEKLGYQWKKSCMNQVTVSTMDRRNNKLIFKINLVEMDEKILVDFRLSKGDGLEFKRHFLKIKGKLSDIVSSQKVWFPVT

Target ORF information:

RefSeq Version	NM_080400.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus checkpoint kinase 1 (Chek1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080400.1

ORF Insert Sequence:

ATGGCAGTGC CTTTTGTGGA AGACTGGGAT TTGGTGCAAA CTTTGGGAGA AGGCGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAATA ACTGAAGAAG CTGTTGCAGT GAAAATTGTA 
GACATGAAGC GGGCCATAGA CTGCCCAGAA AATATTAAGA AAGAGATCTG CATCAATAAG 
ATGTTAAATC ATGAGAATGT AGTGAAATTC TATGGCCACA GGAGGGAAGG CAACATCCAG 
TACCTGTTTC TGGAGTACTG TAGTGGAGGA GAACTCTTTG ATAGAATAGA GCCAGACATA 
GGGATGCCTG AACAAGATGC TCAGAGGTTT TTCCATCAGC TCATGGCGGG GGTGGTTTAT 
CTTCATGGCA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAACCTCCT CTTGGATGAA 
AGGGATAATC TCAAAATCTC TGACTTTGGC TTGGCAACAG TGTTTCGGCA TAATAATCGT 
GAGCGCTTAC TGAACAAGAT GTGTGGGACT TTACCTTATG TCGCTCCAGA GCTTCTAAAG 
AGGAAAGAAT TTCACGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT GCTTACTGCA 
ATGTTGGCTG GAGAATTGCC TTGGGACCAG CCCAGTGATA GCTGTCAAGA ATATTCTGAT 
TGGAAAGAAA AGAAAACCTA TCTCAATCCT TGGAAAAAAA TTGATTCTGC TCCTCTGGCT 
TTGCTTCATA AAATTCTAGT TGAGAATCCA TCAGCAAGGA TCACCATCCC AGACATTAAA 
AAAGATAGAT GGTATAACAA ACCACTTAAC AGAGGAGCGA AGAGGCCACG TGCTACTTCA 
GGTGGTATGT CAGAGTCTTC TAGTGGATTC TCTAAGCACA TTCATTCCAA TTTGGACTTT 
TCTCCAATAA ATAGTGGTTC CAGTGAAGAA AACGTGAAGT TCTCTAGTTC CCAGCCAGAG 
CCTCGGACAG GGCTTTCCTT GTGGGACACT GGCCCCTCAA ATGTGGACAA ACTGGTGCAA 
GGCATCAGTT TTTCCCAGCC TACGTGTCCT GATCATATGC TTGTAAACAG TCAGCTACTT 
GGTACCCCTG GATCTTCACA GAACCCCTGG CAGCGCTTGG TCAAAAGGAT GACACGATTC 
TTCACTAAAT TGGATGCAGA CAAATCTTAC CAATGCCTGA AAGAGACCTT TGAGAAGTTG 
GGCTATCAGT GGAAGAAGAG TTGTATGAAT CAGGTTACTG TATCAACAAT GGATAGAAGA 
AACAATAAGT TGATTTTCAA AATAAATTTG GTAGAAATGG ATGAGAAGAT ACTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGGTTAGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTCAGCG ATATCGTGAG CAGCCAGAAG GTTTGGTTCC CTGTTACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chek1 ( XM_008766042.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764264.1 Rattus norvegicus checkpoint kinase 1 (Chek1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa44166
Clone ID Related Accession (Same CDS sequence)	XM_008766042.2 , XM_008766043.1
Accession Version	XM_008766042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase Chk1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGC CTTTTGTGGA AGACTGGGAT TTGGTGCAAA CTTTGGGAGA AGGCGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAATA ACTGAAGAAG CTGTTGCAGT GAAAATTGTA 
GACATGAAGC GGGCCATAGA CTGCCCAGAA AATATTAAGA AAGAGATCTG CATCAATAAG 
ATGTTAAATC ATGAGAATGT AGTGAAATTC TATGGCCACA GGAGGGAAGG CAACATCCAG 
TACCTGTTTC TGGAGTACTG TAGTGGAGGA GAACTCTTTG ATAGAATAGA GCCAGACATA 
GGGATGCCTG AACAAGATGC TCAGAGGTTT TTCCATCAGC TCATGGCGGG GGTGGTTTAT 
CTTCATGGCA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAACCTCCT CTTGGATGAA 
AGGGATAATC TCAAAATCTC TGACTTTGGC TTGGCAACAG TGTTTCGGCA TAATAATCGT 
GAGCGCTTAC TGAACAAGAT GTGTGGGACT TTACCTTATG TCGCTCCAGA GCTTCTAAAG 
AGGAAAGAAT TTCACGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT GCTTACTGCA 
ATGTTGGCTG GAGAATTGCC TTGGGACCAG CCCAGTGATA GCTGTCAAGA ATATTCTGAT 
TGGAAAGAAA AGAAAACCTA TCTCAATCCT TGGAAAAAAA TTGATTCTGC TCCTCTGGCT 
TTGCTTCATA AAATTCTAGT TGAGAATCCA TCAGCAAGGA TCACCATCCC AGACATTAAA 
AAAGATAGAT GGTACAACAA ACCACTTAAC AGAGGAGCGA AGAGGCCACG TGCTACTTCA 
GGTGGTATGT CAGAGTCTTC TAGTGGATTC TCTAAGCACA TTCATTCCAA TTTGGACTTT 
TCTCCAATAA ATAGTGGTTC CAGTGAAGAA AACGTGAAGT TCTCTAGTTC CCAGCCAGAG 
CCTCGGACAG GGCTTTCCTT GTGGGACACT GGCCCCTCAA ATGTGGACAA ACTGGTGCAA 
GGCATCAGTT TTTCCCAGCC TACGTGTCCT GATCATATGC TTGTAAACAG TCAGCTACTT 
GGTACCCCTG GATCTTCACA GAACCCCTGG CAGCGCTTGG TCAAAAGGAT GACACGATTC 
TTCACTAAAT TGGATGCAGA CAAATCTTAC CAATGCCTGA AAGAGACCTT TGAGAAGTTG 
GGCTATCAGT GGAAGAAGAG TTGTATGAAT CAGGTTACTG TATCAACAAT GGATAGAAGA 
AACAATAAGT TGATTTTCAA AATAAATTTG GTAGAAATGG ATGAGAAGAT ACTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGGTTAGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTCAGCG ATATCGTGAG CAGCCAGAAG GTTTGGTTCC CTGTTACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764264.1
CDS	99..1529
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRITEEAVAVKIVDMKRAIDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEQDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRHNNRERLLNKMCGTLPYVAPELLKRKEFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLNRGAKRPRATSGGMSESSSGFSKHIHSNLDFSPINSGSSEENVKFSSSQPEPRTGLSLWDTGPSNVDKLVQGISFSQPTCPDHMLVNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETFEKLGYQWKKSCMNQVTVSTMDRRNNKLIFKINLVEMDEKILVDFRLSKGDGLEFKRHFLKIKGKLSDIVSSQKVWFPVT

Target ORF information:

RefSeq Version	XM_008766042.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus checkpoint kinase 1 (Chek1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766042.2

ORF Insert Sequence:

ATGGCAGTGC CTTTTGTGGA AGACTGGGAT TTGGTGCAAA CTTTGGGAGA AGGCGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAATA ACTGAAGAAG CTGTTGCAGT GAAAATTGTA 
GACATGAAGC GGGCCATAGA CTGCCCAGAA AATATTAAGA AAGAGATCTG CATCAATAAG 
ATGTTAAATC ATGAGAATGT AGTGAAATTC TATGGCCACA GGAGGGAAGG CAACATCCAG 
TACCTGTTTC TGGAGTACTG TAGTGGAGGA GAACTCTTTG ATAGAATAGA GCCAGACATA 
GGGATGCCTG AACAAGATGC TCAGAGGTTT TTCCATCAGC TCATGGCGGG GGTGGTTTAT 
CTTCATGGCA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAACCTCCT CTTGGATGAA 
AGGGATAATC TCAAAATCTC TGACTTTGGC TTGGCAACAG TGTTTCGGCA TAATAATCGT 
GAGCGCTTAC TGAACAAGAT GTGTGGGACT TTACCTTATG TCGCTCCAGA GCTTCTAAAG 
AGGAAAGAAT TTCACGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT GCTTACTGCA 
ATGTTGGCTG GAGAATTGCC TTGGGACCAG CCCAGTGATA GCTGTCAAGA ATATTCTGAT 
TGGAAAGAAA AGAAAACCTA TCTCAATCCT TGGAAAAAAA TTGATTCTGC TCCTCTGGCT 
TTGCTTCATA AAATTCTAGT TGAGAATCCA TCAGCAAGGA TCACCATCCC AGACATTAAA 
AAAGATAGAT GGTACAACAA ACCACTTAAC AGAGGAGCGA AGAGGCCACG TGCTACTTCA 
GGTGGTATGT CAGAGTCTTC TAGTGGATTC TCTAAGCACA TTCATTCCAA TTTGGACTTT 
TCTCCAATAA ATAGTGGTTC CAGTGAAGAA AACGTGAAGT TCTCTAGTTC CCAGCCAGAG 
CCTCGGACAG GGCTTTCCTT GTGGGACACT GGCCCCTCAA ATGTGGACAA ACTGGTGCAA 
GGCATCAGTT TTTCCCAGCC TACGTGTCCT GATCATATGC TTGTAAACAG TCAGCTACTT 
GGTACCCCTG GATCTTCACA GAACCCCTGG CAGCGCTTGG TCAAAAGGAT GACACGATTC 
TTCACTAAAT TGGATGCAGA CAAATCTTAC CAATGCCTGA AAGAGACCTT TGAGAAGTTG 
GGCTATCAGT GGAAGAAGAG TTGTATGAAT CAGGTTACTG TATCAACAAT GGATAGAAGA 
AACAATAAGT TGATTTTCAA AATAAATTTG GTAGAAATGG ATGAGAAGAT ACTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGGTTAGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTCAGCG ATATCGTGAG CAGCCAGAAG GTTTGGTTCC CTGTTACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chek1 ( XM_008766043.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764265.1 Rattus norvegicus checkpoint kinase 1 (Chek1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa44166
Clone ID Related Accession (Same CDS sequence)	XM_008766042.2 , XM_008766043.1
Accession Version	XM_008766043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase Chk1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGC CTTTTGTGGA AGACTGGGAT TTGGTGCAAA CTTTGGGAGA AGGCGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAATA ACTGAAGAAG CTGTTGCAGT GAAAATTGTA 
GACATGAAGC GGGCCATAGA CTGCCCAGAA AATATTAAGA AAGAGATCTG CATCAATAAG 
ATGTTAAATC ATGAGAATGT AGTGAAATTC TATGGCCACA GGAGGGAAGG CAACATCCAG 
TACCTGTTTC TGGAGTACTG TAGTGGAGGA GAACTCTTTG ATAGAATAGA GCCAGACATA 
GGGATGCCTG AACAAGATGC TCAGAGGTTT TTCCATCAGC TCATGGCGGG GGTGGTTTAT 
CTTCATGGCA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAACCTCCT CTTGGATGAA 
AGGGATAATC TCAAAATCTC TGACTTTGGC TTGGCAACAG TGTTTCGGCA TAATAATCGT 
GAGCGCTTAC TGAACAAGAT GTGTGGGACT TTACCTTATG TCGCTCCAGA GCTTCTAAAG 
AGGAAAGAAT TTCACGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT GCTTACTGCA 
ATGTTGGCTG GAGAATTGCC TTGGGACCAG CCCAGTGATA GCTGTCAAGA ATATTCTGAT 
TGGAAAGAAA AGAAAACCTA TCTCAATCCT TGGAAAAAAA TTGATTCTGC TCCTCTGGCT 
TTGCTTCATA AAATTCTAGT TGAGAATCCA TCAGCAAGGA TCACCATCCC AGACATTAAA 
AAAGATAGAT GGTACAACAA ACCACTTAAC AGAGGAGCGA AGAGGCCACG TGCTACTTCA 
GGTGGTATGT CAGAGTCTTC TAGTGGATTC TCTAAGCACA TTCATTCCAA TTTGGACTTT 
TCTCCAATAA ATAGTGGTTC CAGTGAAGAA AACGTGAAGT TCTCTAGTTC CCAGCCAGAG 
CCTCGGACAG GGCTTTCCTT GTGGGACACT GGCCCCTCAA ATGTGGACAA ACTGGTGCAA 
GGCATCAGTT TTTCCCAGCC TACGTGTCCT GATCATATGC TTGTAAACAG TCAGCTACTT 
GGTACCCCTG GATCTTCACA GAACCCCTGG CAGCGCTTGG TCAAAAGGAT GACACGATTC 
TTCACTAAAT TGGATGCAGA CAAATCTTAC CAATGCCTGA AAGAGACCTT TGAGAAGTTG 
GGCTATCAGT GGAAGAAGAG TTGTATGAAT CAGGTTACTG TATCAACAAT GGATAGAAGA 
AACAATAAGT TGATTTTCAA AATAAATTTG GTAGAAATGG ATGAGAAGAT ACTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGGTTAGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTCAGCG ATATCGTGAG CAGCCAGAAG GTTTGGTTCC CTGTTACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764265.1
CDS	116..1546
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRITEEAVAVKIVDMKRAIDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEQDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRHNNRERLLNKMCGTLPYVAPELLKRKEFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLNRGAKRPRATSGGMSESSSGFSKHIHSNLDFSPINSGSSEENVKFSSSQPEPRTGLSLWDTGPSNVDKLVQGISFSQPTCPDHMLVNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETFEKLGYQWKKSCMNQVTVSTMDRRNNKLIFKINLVEMDEKILVDFRLSKGDGLEFKRHFLKIKGKLSDIVSSQKVWFPVT

Target ORF information:

RefSeq Version	XM_008766043.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus checkpoint kinase 1 (Chek1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766043.1

ORF Insert Sequence:

ATGGCAGTGC CTTTTGTGGA AGACTGGGAT TTGGTGCAAA CTTTGGGAGA AGGCGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAATA ACTGAAGAAG CTGTTGCAGT GAAAATTGTA 
GACATGAAGC GGGCCATAGA CTGCCCAGAA AATATTAAGA AAGAGATCTG CATCAATAAG 
ATGTTAAATC ATGAGAATGT AGTGAAATTC TATGGCCACA GGAGGGAAGG CAACATCCAG 
TACCTGTTTC TGGAGTACTG TAGTGGAGGA GAACTCTTTG ATAGAATAGA GCCAGACATA 
GGGATGCCTG AACAAGATGC TCAGAGGTTT TTCCATCAGC TCATGGCGGG GGTGGTTTAT 
CTTCATGGCA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAACCTCCT CTTGGATGAA 
AGGGATAATC TCAAAATCTC TGACTTTGGC TTGGCAACAG TGTTTCGGCA TAATAATCGT 
GAGCGCTTAC TGAACAAGAT GTGTGGGACT TTACCTTATG TCGCTCCAGA GCTTCTAAAG 
AGGAAAGAAT TTCACGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT GCTTACTGCA 
ATGTTGGCTG GAGAATTGCC TTGGGACCAG CCCAGTGATA GCTGTCAAGA ATATTCTGAT 
TGGAAAGAAA AGAAAACCTA TCTCAATCCT TGGAAAAAAA TTGATTCTGC TCCTCTGGCT 
TTGCTTCATA AAATTCTAGT TGAGAATCCA TCAGCAAGGA TCACCATCCC AGACATTAAA 
AAAGATAGAT GGTACAACAA ACCACTTAAC AGAGGAGCGA AGAGGCCACG TGCTACTTCA 
GGTGGTATGT CAGAGTCTTC TAGTGGATTC TCTAAGCACA TTCATTCCAA TTTGGACTTT 
TCTCCAATAA ATAGTGGTTC CAGTGAAGAA AACGTGAAGT TCTCTAGTTC CCAGCCAGAG 
CCTCGGACAG GGCTTTCCTT GTGGGACACT GGCCCCTCAA ATGTGGACAA ACTGGTGCAA 
GGCATCAGTT TTTCCCAGCC TACGTGTCCT GATCATATGC TTGTAAACAG TCAGCTACTT 
GGTACCCCTG GATCTTCACA GAACCCCTGG CAGCGCTTGG TCAAAAGGAT GACACGATTC 
TTCACTAAAT TGGATGCAGA CAAATCTTAC CAATGCCTGA AAGAGACCTT TGAGAAGTTG 
GGCTATCAGT GGAAGAAGAG TTGTATGAAT CAGGTTACTG TATCAACAAT GGATAGAAGA 
AACAATAAGT TGATTTTCAA AATAAATTTG GTAGAAATGG ATGAGAAGAT ACTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGGTTAGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTCAGCG ATATCGTGAG CAGCCAGAAG GTTTGGTTCC CTGTTACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

hHR23A is required to control the basal turnover of Chk1.
Cellular signalling27(11)2304-13(2015 Nov)
Tan X,Liang RY,Chuang SM

Regulated degradation of Chk1 by chaperone-mediated autophagy in response to DNA damage.
Nature communications66823(2015 Apr)
Park C,Suh Y,Cuervo AM

A bioinformatics approach identifies signal transducer and activator of transcription-3 and checkpoint kinase 1 as upstream regulators of kidney injury molecule-1 after kidney injury.
Journal of the American Society of Nephrology : JASN25(1)105-18(2014 Jan)
Ajay AK,Kim TM,Ramirez-Gonzalez V,Park PJ,Frank DA,Vaidya VS

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma na?ve to therapy.
Molecular bioSystems9(6)1108-16(2013 Jun)
Pieragostino D,Agnifili L,Fasanella V,D'Aguanno S,Mastropasqua R,Di Ilio C,Sacchetta P,Urbani A,Del Boccio P

CK1? kinase activity is modulated by Chk1-mediated phosphorylation.
PloS one8(7)e68803(2013)
Bischof J,Randoll SJ,S??ner N,Henne-Bruns D,Pinna LA,Knippschild U

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Chek1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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