Atp6v1b2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp6v1b2
Entrez Gene ID	117596
Full Name	ATPase H+ transporting V1 subunit B2
Synonyms	Atp6b1b2,Atp6b2,Vatb
General protein information	Preferred Names ATPase H+ transporting V1 subunit B2 Names V-type proton ATPase subunit B, brain isoform ATPase, H transporting, lysosomal V1 subunit B2 ATPase, H+ transporting, V1 subunit B ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta 56/58 kDa V-ATPase subunit B 2 endomembrane proton pump 58 kDa subunit vacuolar H+ATPase B2 vacuolar proton pump subunit B 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p14
Summary	subunit of vacuolar H(+)-ATPase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_057213.2	NP_476561.1	V-type proton ATPase subunit B, brain isoform

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATP6V1B2	NP_001684.2
Pan troglodytes (chimpanzee)	ATP6V1B2	XP_519638.2
Saccharomyces cerevisiae (baker's yeast)	VMA2	NP_009685.3
Arabidopsis thaliana (thale cress)	AT1G20260	NP_173451.5
Oryza sativa (rice)	Os06g0568200	NP_001057902.1
Rattus norvegicus (Norway rat)	Atp6v1b2	NP_476561.1
Caenorhabditis elegans (roundworm)	spe-5	NP_491518.1
Magnaporthe oryzae (rice blast fungus)	MGG_03244	XP_003716730.1
Xenopus tropicalis (tropical clawed frog)	atp6v1b2	NP_001072182.1
	ATP6V1B2	XP_001100869.1
Canis lupus familiaris (dog)	ATP6V1B2	XP_543263.2
Bos taurus (cattle)	ATP6V1B2	NP_788844.2
Mus musculus (house mouse)	Atp6v1b2	NP_031535.2
Arabidopsis thaliana (thale cress)	AT1G76030	NP_177729.1
Gallus gallus (chicken)	ATP6V1B2	XP_424534.3
Drosophila melanogaster (fruit fly)	Vha55	NP_001163597.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002884	XP_312029.3
Schizosaccharomyces pombe (fission yeast)	vma2	NP_594623.1
Neurospora crassa	NCU08515	XP_963165.1
Danio rerio (zebrafish)	atp6v1b2	NP_878299.1
Kluyveromyces lactis	KLLA0D09152g	XP_453470.1
Eremothecium gossypii	AGOS_ADL380W	NP_983716.1

Gene Ontology
Bibliography

Related articles in PubMed

V-ATPase subunit ATP6AP1 (Ac45) regulates osteoclast differentiation, extracellular acidification, lysosomal trafficking, and protease exocytosis in osteoclast-mediated bone resorption.
Yang DQ, Feng S, Chen W, Zhao H, Paulson C, Li YP
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research27(8)1695-707(2012 Aug)

Murine ameloblasts are immunonegative for Tcirg1, the v-H-ATPase subunit essential for the osteoclast plasma proton pump.
Bronckers AL, Lyaruu DM, Bervoets TJ, Medina JF, DenBesten P, Richter J, Everts V
Bone50(4)901-8(2012 Apr)

Vacuolar ATPase regulates surfactant secretion in rat alveolar type II cells by modulating lamellar body calcium.
Chintagari NR, Mishra A, Su L, Wang Y, Ayalew S, Hartson SD, Liu L
PloS one5(2)e9228(2010 Feb)

Long-term regulation of vacuolar H(+)-ATPase by angiotensin II in proximal tubule cells.
Carraro-Lacroix LR, Girardi AC, Malnic G
Pflugers Archiv : European journal of physiology458(5)969-79(2009 Sep)

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that silencing of V-ATPase a1 and B2 subunits decreased stimulated surfactant secretion.
Title: Vacuolar ATPase regulates surfactant secretion in rat alveolar type II cells by modulating lamellar body calcium.

Long-term exposure of proximal tubule cells to angiotensin II causes upregulation of H(+)-ATPase activity due to increased B2-subunit cell-surface expression, which is dependent on mechanisms involving tyrosine kinase, p38 MAPK, and PI3K activation.
Title: Long-term regulation of vacuolar H(+)-ATPase by angiotensin II in proximal tubule cells.

In mice that lack a functional B1 subunit of the V-ATPase, sAC was colocalized apically in A-IC along with V-ATPase containing the alternative B2 subunit isoform.
Title: Association of soluble adenylyl cyclase with the V-ATPase in renal epithelial cells.

expressed in most, if not all, proton-secreting cells in the epithelium lining the nephron and the collecting ducts, and it is also present in proton-secreting clear cells of the epididymis
Title: Expression of the 56-kDa B2 subunit isoform of the vacuolar H(+)-ATPase in proton-secreting cells of the kidney and epididymis.

The following Atp6v1b2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp6v1b2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11665	NM_057213.2 Latest version!	Rattus norvegicus ATPase H+ transporting V1 subunit B2 (Atp6v1b2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp6v1b2 ( NM_057213.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_476561.1 Rattus norvegicus ATPase H+ transporting V1 subunit B2 (Atp6v1b2), mRNA.

Price & Availability↑

CloneID	ORa11665
Clone ID Related Accession (Same CDS sequence)	NM_057213.2
Accession Version	NM_057213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	V-type proton ATPase subunit B, brain isoform
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085714.1. On Nov 15, 2004 this sequence version replaced NM_057213.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085714.1, Y12635.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTTGC GAGCGATGCG GGGAATCGTG AACGGGGCCG CGCCCGAGCT GCCCGTGCCC 
ACCGGTGGGC CGATGGCCGG AGCTCGGGAG CAGGCGCTGG CGGTGAGCCG GAACTACCTC 
TCCCAGCCTC GTCTCACCTA CAAGACTGTC TCTGGAGTGA ATGGTCCACT AGTGATCTTA 
GATCATGTAA AGTTTCCCAG ATATGCTGAG ATTGTCCACT TGACATTACC AGATGGCACA 
AAAAGAAGTG GGCAAGTTCT AGAAGTTAGT GGCTCCAAAG CCGTGGTTCA GGTATTTGAA 
GGAACATCCG GCATAGATGC CAAGAAAACA TCCTGTGAGT TTACTGGAGA TATTCTCCGC 
ACACCAGTGT CTGAGGATAT GCTTGGTCGA GTATTCAATG GATCAGGAAA ACCCATTGAC 
CGAGGTCCTG TGGTGTTGGC CGAAGACTTC CTTGACATCA TGGGTCAGCC AATCAACCCT 
CAGTGTCGCA TCTACCCAGA AGAGATGATT CAGACGGGCA TTTCTGCCAT CGATGGCATG 
AACAGTATTG CGAGGGGACA GAAAATCCCC ATCTTTTCTG CTGCCGGGTT ACCACACAAC 
GAGATTGCAG CTCAGATCTG TCGCCAGGCT GGTTTGGTAA AGAAATCCAA AGACGTGGTA 
GACTACAGTG AAGAAAACTT TGCCATTGTG TTTGCTGCTA TGGGAGTAAA CATGGAAACA 
GCCCGGTTCT TCAAATCTGA CTTTGAAGAA AATGGCTCAA TGGACAATGT CTGCCTTTTC 
TTGAATCTGG CTAATGACCC AACTATCGAG AGGATCATCA CTCCTCGCCT GGCTCTGACC 
ACCGCTGAGT TTCTGGCTTA CCAGTGTGAG AAGCATGTCC TGGTCATCCT GACAGATATG 
AGTTCTTACG CTGAAGCACT TCGAGAGGTT TCAGCTGCCA GGGAAGAGGT TCCTGGTCGG 
CGAGGCTTCC CCGGCTACAT GTATACGGAT TTAGCCACCA TCTATGAACG CGCTGGGCGA 
GTGGAAGGTA GAAATGGCTC TATTACCCAA ATCCCTATTC TCACCATGCC CAATGATGAT 
ATCACTCATC CTATCCCTGA CTTGACTGGG TATATTACTG AGGGCCAGAT CTATGTGGAC 
AGACAGCTGC ACAACAGACA GATTTACCCT CCTATTAATG TGCTGCCCTC ACTCTCTCGG 
TTAATGAAGT CAGCTATTGG AGAAGGAATG ACCAGGAAGG ATCATGCTGA TGTGTCTAAC 
CAGTTGTACG CATGCTATGC TATCGGTAAG GATGTGCAAG CCATGAAAGC TGTGGTGGGA 
GAAGAAGCCC TGACCTCAGA TGACCTCCTT TACTTGGAAT TTCTGCAGAA GTTTGAGAAA 
AACTTCATTA CTCAGGGTCC CTATGAAAAT CGAACTGTCT ATGAGACTTT GGACATTGGC 
TGGCAGTTGC TTCGAATCTT CCCCAAAGAA ATGCTGAAGA GGATCCCTCA GAGTACCCTG 
AGCGAATTTT ACCCTCGAGA CTCTGCAAAG CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476561.1
CDS	14..1549
Translation	MALRAMRGIVNGAAPELPVPTGGPMAGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFEKNFITQGPYENRTVYETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH

Target ORF information:

RefSeq Version	NM_057213.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase H+ transporting V1 subunit B2 (Atp6v1b2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057213.2

ORF Insert Sequence:

ATGGCGTTGC GAGCGATGCG GGGAATCGTG AACGGGGCCG CGCCCGAGCT GCCCGTGCCC 
ACCGGTGGGC CGATGGCCGG AGCTCGGGAG CAGGCGCTGG CGGTGAGCCG GAACTACCTC 
TCCCAGCCTC GTCTCACCTA CAAGACTGTC TCTGGAGTGA ATGGTCCACT AGTGATCTTA 
GATCATGTAA AGTTTCCCAG ATATGCTGAG ATTGTCCACT TGACATTACC AGATGGCACA 
AAAAGAAGTG GGCAAGTTCT AGAAGTTAGT GGCTCCAAAG CCGTGGTTCA GGTATTTGAA 
GGAACATCCG GCATAGATGC CAAGAAAACA TCCTGTGAGT TTACTGGAGA TATTCTCCGC 
ACACCAGTGT CTGAGGATAT GCTTGGTCGA GTATTCAATG GATCAGGAAA ACCCATTGAC 
CGAGGTCCTG TGGTGTTGGC CGAAGACTTC CTTGACATCA TGGGTCAGCC AATCAACCCT 
CAGTGTCGCA TCTACCCAGA AGAGATGATT CAGACGGGCA TTTCTGCCAT CGATGGCATG 
AACAGTATTG CGAGGGGACA GAAAATCCCC ATCTTTTCTG CTGCCGGGTT ACCACACAAC 
GAGATTGCAG CTCAGATCTG TCGCCAGGCT GGTTTGGTAA AGAAATCCAA AGACGTGGTA 
GACTACAGTG AAGAAAACTT TGCCATTGTG TTTGCTGCTA TGGGAGTAAA CATGGAAACA 
GCCCGGTTCT TCAAATCTGA CTTTGAAGAA AATGGCTCAA TGGACAATGT CTGCCTTTTC 
TTGAATCTGG CTAATGACCC AACTATCGAG AGGATCATCA CTCCTCGCCT GGCTCTGACC 
ACCGCTGAGT TTCTGGCTTA CCAGTGTGAG AAGCATGTCC TGGTCATCCT GACAGATATG 
AGTTCTTACG CTGAAGCACT TCGAGAGGTT TCAGCTGCCA GGGAAGAGGT TCCTGGTCGG 
CGAGGCTTCC CCGGCTACAT GTATACGGAT TTAGCCACCA TCTATGAACG CGCTGGGCGA 
GTGGAAGGTA GAAATGGCTC TATTACCCAA ATCCCTATTC TCACCATGCC CAATGATGAT 
ATCACTCATC CTATCCCTGA CTTGACTGGG TATATTACTG AGGGCCAGAT CTATGTGGAC 
AGACAGCTGC ACAACAGACA GATTTACCCT CCTATTAATG TGCTGCCCTC ACTCTCTCGG 
TTAATGAAGT CAGCTATTGG AGAAGGAATG ACCAGGAAGG ATCATGCTGA TGTGTCTAAC 
CAGTTGTACG CATGCTATGC TATCGGTAAG GATGTGCAAG CCATGAAAGC TGTGGTGGGA 
GAAGAAGCCC TGACCTCAGA TGACCTCCTT TACTTGGAAT TTCTGCAGAA GTTTGAGAAA 
AACTTCATTA CTCAGGGTCC CTATGAAAAT CGAACTGTCT ATGAGACTTT GGACATTGGC 
TGGCAGTTGC TTCGAATCTT CCCCAAAGAA ATGCTGAAGA GGATCCCTCA GAGTACCCTG 
AGCGAATTTT ACCCTCGAGA CTCTGCAAAG CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

V-ATPase subunit ATP6AP1 (Ac45) regulates osteoclast differentiation, extracellular acidification, lysosomal trafficking, and protease exocytosis in osteoclast-mediated bone resorption.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research27(8)1695-707(2012 Aug)
Yang DQ,Feng S,Chen W,Zhao H,Paulson C,Li YP

Murine ameloblasts are immunonegative for Tcirg1, the v-H-ATPase subunit essential for the osteoclast plasma proton pump.
Bone50(4)901-8(2012 Apr)
Bronckers AL,Lyaruu DM,Bervoets TJ,Medina JF,DenBesten P,Richter J,Everts V

Vacuolar ATPase regulates surfactant secretion in rat alveolar type II cells by modulating lamellar body calcium.
PloS one5(2)e9228(2010 Feb)
Chintagari NR,Mishra A,Su L,Wang Y,Ayalew S,Hartson SD,Liu L

Long-term regulation of vacuolar H(+)-ATPase by angiotensin II in proximal tubule cells.
Pflugers Archiv : European journal of physiology458(5)969-79(2009 Sep)
Carraro-Lacroix LR,Girardi AC,Malnic G

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Journal of proteome research8(3)1304-14(2009 Mar)
Gonzalez-Begne M,Lu B,Han X,Hagen FK,Hand AR,Melvin JE,Yates JR

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Atp6v1b2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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