Kif5b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kif5b
Entrez Gene ID	117550
Full Name	kinesin family member 5B
Synonyms	Khc
General protein information	Preferred Names kinesin family member 5B Names kinesin-1 heavy chain UKHC conventional kinesin heavy chain ubiquitous kinesin heavy chain
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.1
Summary	mouse homolog is the heavy chain of kinesin; essential for mitochondrial and lysosomal dispersion [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_057202.1	NP_476550.1	kinesin-1 heavy chain
XM_006254005.1	XP_006254067.1	kinesin-1 heavy chain isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIF5B	NP_004512.1
Bos taurus (cattle)	KIF5B	NP_001179294.1
Canis lupus familiaris (dog)	KIF5B	XP_003638926.1
Danio rerio (zebrafish)	kif5ba	XP_005171465.1
Caenorhabditis elegans (roundworm)	unc-116	NP_498842.1
Neurospora crassa	NCU09730	XP_964432.2
	KIF5B	XP_002805653.1
Drosophila melanogaster (fruit fly)	Khc	NP_476590.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000561	XP_310522.2
Pan troglodytes (chimpanzee)	KIF5B	XP_507730.2
Mus musculus (house mouse)	Kif5b	NP_032474.2
Rattus norvegicus (Norway rat)	Kif5b	NP_476550.1
Gallus gallus (chicken)	KIF5B	XP_004939228.1
Danio rerio (zebrafish)	kif5bb	XP_002664065.1
Magnaporthe oryzae (rice blast fungus)	MGG_01757	XP_003714755.1
Xenopus tropicalis (tropical clawed frog)	kif5b	NP_001122126.1

Gene Ontology
Bibliography

Related articles in PubMed

MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons.
Gumy LF, Katrukha EA, Grigoriev I, Jaarsma D, Kapitein LC, Akhmanova A, Hoogenraad CC
Neuron94(2)347-362.e7(2017 Apr)

Hypertrophy induced KIF5B controls mitochondrial localization and function in neonatal rat cardiomyocytes.
Tigchelaar W, de Jong AM, Bloks VW, van Gilst WH, de Boer RA, Sillj? HH
Journal of molecular and cellular cardiology9770-81(2016 Aug)

Kinesin 1 Drives Autolysosome Tubulation.
Du W, Su QP, Chen Y, Zhu Y, Jiang D, Rong Y, Zhang S, Zhang Y, Ren H, Zhang C, Wang X, Gao N, Wang Y, Sun L, Sun Y, Yu L
Developmental cell37(4)326-336(2016 05)

The Basic Domain of Herpes Simplex Virus 1 pUS9 Recruits Kinesin-1 To Facilitate Egress from Neurons.
Diefenbach RJ, Davis A, Miranda-Saksena M, Fernandez MA, Kelly BJ, Jones CA, LaVail JH, Xue J, Lai J, Cunningham AL
Journal of virology90(4)2102-11(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that KIF5B contributes to mitochondrial localization and function in cardiomyocytes and may play a role in pathological hypertrophic responses in vivo.
Title: Hypertrophy induced KIF5B controls mitochondrial localization and function in neonatal rat cardiomyocytes.

clathrin promotes formation of phosphatidylinositol(4,5)diphosphate-enriched microdomains, which are required for clustering of KIF5B.
Title: Kinesin 1 Drives Autolysosome Tubulation.

The authors used an in vitro pulldown assay to define a series of five arginine residues within the conserved pUS9 basic domain of herpes simplex virus 1 that were essential for binding the molecular motor kinesin-1.
Title: The Basic Domain of Herpes Simplex Virus 1 pUS9 Recruits Kinesin-1 To Facilitate Egress from Neurons.

Diabetes seems to change spinal cord KIF5B and SYD mRNA and sciatic nerves KIF5B content and exercise training modifies it, which may be attributable to the training-induced decreased hyperglycemia.
Title: Treadmill training modifies KIF5B motor protein in the STZ-induced diabetic rat spinal cord and sciatic nerve.

dysferlin-containing vesicles are transported along microtubles via kinesin heavy chain isoform KIF5B, and undergo rapid microtubule- and KIF5B-dependent fusion to form extremely large vesicles following cellular wounding in skeletal muscle cells.
Title: Membrane damage-induced vesicle-vesicle fusion of dysferlin-containing vesicles in muscle cells requires microtubules and kinesin.

The following Kif5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kif5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11601	NM_057202.1 Latest version!	Rattus norvegicus kinesin family member 5B (Kif5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa11601	XM_006254005.1 Latest version!	Rattus norvegicus kinesin family member 5B (Kif5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kif5b ( NM_057202.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_476550.1 Rattus norvegicus kinesin family member 5B (Kif5b), mRNA.

Price & Availability↑

CloneID	ORa11601
Clone ID Related Accession (Same CDS sequence)	NM_057202.1 , XM_006254005.1
Accession Version	NM_057202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-1 heavy chain
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ309275.1. On Dec 22, 2005 this sequence version replaced XM_341538.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ309275.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACC CGGCGGAGTG CAACATCAAA GTGATGTGTC GCTTCAGACC TCTCAACGAG 
TCTGAAGTGA ACCGCGGCGA CAAGTATGTC GCCAAGTTCC AGGGAGAAGA CACGGTGATG 
ATTGCGTCCA AGCCTTATGC ATTTGATCGT GTGTTCCAGT CAAGCACATC TCAAGAGCAA 
GTATACAACG ATTGTGCAAA GAAGATTGTT AAAGATGTTC TTGAGGGCTA TAATGGAACA 
ATATTTGCAT ATGGACAAAC ATCATCTGGA AAGACCCACA CAATGGAGGG TAAACTTCAT 
GATCCAGAAG GCATGGGGAT TATTCCAAGA ATAGTGCAAG ATATTTTTAA TTATATTTAC 
TCCATGGATG AAAATTTGGA ATTTCATATT AAGGTTTCAT ATTTTGAAAT ATATTTGGAT 
AAGATAAGGG ACCTGTTAGA TGTTTCAAAG ACTAACCTTT CAGTCCATGA AGACAAAAAC 
CGTGTTCCCT ATGTAAAGGG GTGCACAGAG CGTTTTGTGT GTAGTCCAGA TGAAGTTATG 
GATACCATAG ATGAAGGGAA ATCCAACAGA CACGTAGCAG TTACAAATAT GAATGAACAT 
AGCTCTAGGA GTCACAGTAT ATTTCTTATC AATGTAAAGC AAGAGAATAC ACAGACAGAA 
CAGAAACTCA GTGGGAAGCT TTATCTGGTT GATTTAGCTG GAAGTGAAAA GGTTAGTAAA 
ACTGGAGCTG AGGGTGCCGT GCTGGATGAA GCTAAGAACA TCAACAAGTC ACTTTCTGCA 
CTTGGAAATG TCATTTCTGC TTTGGCAGAA GGCAGTACCT ATGTTCCTTA TCGAGACAGT 
AAAATGACCA GAATTCTTCA AGATTCATTA GGTGGCAACT GTAGGACCAC TATTGTCATT 
TGCTGCTCTC CGTCATCTTA CAATGAGTCT GAGACAAAGT CAACACTCCT CTTTGGTCAA 
AGGGCCAAAA CAATAAAGAA CACAGTCTGT GTCAACGTAG AGTTAACTGC AGAGCAGTGG 
AAAAAGAAGT ATGAAAAAGA AAAGGAAAAA AATAAGACTC TACGGAACAC CATTCAGTGG 
CTGGAAAACG AGCTCAACCG ATGGCGTAAC GGGGAAACAG TGCCTATTGA TGAGCAGTTT 
GACAAAGAGA AGGCTAATTT GGAAGCCTTC ACAGCAGATA AAGATGTTGC TATTACCAAT 
GACAAACCGG CTGCTGCAAT CGGAATGGCT GGTAGTTTTA CAGATGCTGA GAGAAGAAAG 
TGTGAAGAAG AAATTGCTAA ATTGTATAAA CAACTTGATG ACAAGGATGA AGAGATTAAC 
CAACAGAGCC AACTGGTGGA GAAATTGAAG ACACAAATGT TGGATCAGGA AGAGCTTCTG 
GCATCAACCA GAAGGGATCA AGATAATATG CAAGCTGAAC TGAATCGCCT CCAAGCAGAA 
AATGATGCTT CTAAAGAAGA AGTCAAAGAA GTTTTACAGG CCTTAGAGGA GCTGGCTGTT 
AATTATGATC AGAAGTCTCA GGAAGTTGAA GACAAAACAA AGGAATATGA ATTGCTTAGT 
GATGAATTGA ATCAAAAATC TGCAACTTTA GCAAGTATTG ATGCTGAGCT TCAGAAGCTG 
AAGGAAATGA CCAACCACCA GAAGAAACGA GCAGCTGAAA TGATGGCATC ATTACTAAAA 
GACCTTGCAG AAATAGGAAT TGCTGTGGGA AATAATGATG TAAAGCAACC GGAGGGGACT 
GGTATGATAG ATGAAGAGTT TACTGTTGCA AGGCTCTACA TTAGCAAAAT GAAATCAGAA 
GTAAAAACCA TGGTGAAACG CTGCAAACAG CTAGAAAGCA CACAGACTGA GAGCAACAAA 
AAAATGGAAG AAAATGAGAA GGAGTTAGCA GCATGCCAGC TTCGTATCTC CCAACATGAA 
GCCAAAATCA AGTCACTGAC TGAATACCTT CAAAATGTAG AACAAAAGAA GAGACAGCTG 
GAGGAATCTG TGGACTCCCT TGGTGAGGAG CTAGTCCAAC TTCGAGCACA AGAGAAAGTC 
CATGAAATGG AAAAAGAGCA CTTGAACAAG GTTCAGACTG CAAATGAAGT CAAGCAAGCT 
GTTGAACAGC AGATCCAGAG TCACAGAGAA ACCCACCAAA AACAAATCAG TAGTTTACGA 
GATGAAGTTG AGGCAAAGGA AAAGCTAATC ACTGATCTCC AAGACCAAAA CCAGAAGATG 
GTGTTGGAAC AGGAGCGGCT AAGGGTGGAG CATGAGAGGC TAAAGGCTGT AGACCAGGAG 
AAGAGCAGGA AGCTGCATGA GCTCACGGTT ATGCAAGACA GACGAGAACA AGCAAGGCAA 
GATTTGAAGG GGTTGGAAGA GACGGTGGCA AAAGAACTTC AGACTTTACA CAACCTGCGC 
AAGCTCTTTG TTCAGGACTT GGCTACCAGG GTGAAAAAGA GCGCTGAGGT GGACTCAGAC 
GACACTGGCG GCAGTGCTGC ACAGAAGCAG AAAATCTCCT TCCTTGAAAA CAATCTGGAG 
CAGCTCACCA AAGTGCACAA GCAGTTGGTG CGTGATAATG CAGATCTTCG CTGTGAACTT 
CCTAAGTTAG AGAAACGACT TAGAGCTACT GCAGAGAGAG TGAAAGCTTT GGAGTCAGCC 
CTGAAAGAAG CCAAAGAAAA TGCATCTCGA GACCGCAAAC GCTATCAGCA AGAAGTAGAC 
AGGATAAAGG AAGCAGTCAG GTCAAAGAAT ATGGCCAGAA GGGGACACTC TGCACAGATT 
GCAAAACCTA TCCGTCCTGG ACAGCATCCA GCAGCCTCTC CAACTCACCC CGGTGCAGTT 
CGTGGAGGAG GCTCATTTGT TCAGAACAAC CAGCCAGTGG GGCTTCGAGG TGGTGGAGGC 
AAGCAGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476550.1
CDS	1..2892
Translation	MADPAECNIKVMCRFRPLNESEVNRGDKYVAKFQGEDTVMIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKTLRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTADKDVAITNDKPAAAIGMAGSFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDSLGEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKEKLITDLQDQNQKMVLEQERLRVEHERLKAVDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEVDSDDTGGSAAQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKNMARRGHSAQIAKPIRPGQHPAASPTHPGAVRGGGSFVQNNQPVGLRGGGGKQA

Target ORF information:

RefSeq Version	NM_057202.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 5B (Kif5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057202.1

ORF Insert Sequence:

ATGGCGGACC CGGCGGAGTG CAACATCAAA GTGATGTGTC GCTTCAGACC TCTCAACGAG 
TCTGAAGTGA ACCGCGGCGA CAAGTATGTC GCCAAGTTCC AGGGAGAAGA CACGGTGATG 
ATTGCGTCCA AGCCTTATGC ATTTGATCGT GTGTTCCAGT CAAGCACATC TCAAGAGCAA 
GTATACAACG ATTGTGCAAA GAAGATTGTT AAAGATGTTC TTGAGGGCTA TAATGGAACA 
ATATTTGCAT ATGGACAAAC ATCATCTGGA AAGACCCACA CAATGGAGGG TAAACTTCAT 
GATCCAGAAG GCATGGGGAT TATTCCAAGA ATAGTGCAAG ATATTTTTAA TTATATTTAC 
TCCATGGATG AAAATTTGGA ATTTCATATT AAGGTTTCAT ATTTTGAAAT ATATTTGGAT 
AAGATAAGGG ACCTGTTAGA TGTTTCAAAG ACTAACCTTT CAGTCCATGA AGACAAAAAC 
CGTGTTCCCT ATGTAAAGGG GTGCACAGAG CGTTTTGTGT GTAGTCCAGA TGAAGTTATG 
GATACCATAG ATGAAGGGAA ATCCAACAGA CACGTAGCAG TTACAAATAT GAATGAACAT 
AGCTCTAGGA GTCACAGTAT ATTTCTTATC AATGTAAAGC AAGAGAATAC ACAGACAGAA 
CAGAAACTCA GTGGGAAGCT TTATCTGGTT GATTTAGCTG GAAGTGAAAA GGTTAGTAAA 
ACTGGAGCTG AGGGTGCCGT GCTGGATGAA GCTAAGAACA TCAACAAGTC ACTTTCTGCA 
CTTGGAAATG TCATTTCTGC TTTGGCAGAA GGCAGTACCT ATGTTCCTTA TCGAGACAGT 
AAAATGACCA GAATTCTTCA AGATTCATTA GGTGGCAACT GTAGGACCAC TATTGTCATT 
TGCTGCTCTC CGTCATCTTA CAATGAGTCT GAGACAAAGT CAACACTCCT CTTTGGTCAA 
AGGGCCAAAA CAATAAAGAA CACAGTCTGT GTCAACGTAG AGTTAACTGC AGAGCAGTGG 
AAAAAGAAGT ATGAAAAAGA AAAGGAAAAA AATAAGACTC TACGGAACAC CATTCAGTGG 
CTGGAAAACG AGCTCAACCG ATGGCGTAAC GGGGAAACAG TGCCTATTGA TGAGCAGTTT 
GACAAAGAGA AGGCTAATTT GGAAGCCTTC ACAGCAGATA AAGATGTTGC TATTACCAAT 
GACAAACCGG CTGCTGCAAT CGGAATGGCT GGTAGTTTTA CAGATGCTGA GAGAAGAAAG 
TGTGAAGAAG AAATTGCTAA ATTGTATAAA CAACTTGATG ACAAGGATGA AGAGATTAAC 
CAACAGAGCC AACTGGTGGA GAAATTGAAG ACACAAATGT TGGATCAGGA AGAGCTTCTG 
GCATCAACCA GAAGGGATCA AGATAATATG CAAGCTGAAC TGAATCGCCT CCAAGCAGAA 
AATGATGCTT CTAAAGAAGA AGTCAAAGAA GTTTTACAGG CCTTAGAGGA GCTGGCTGTT 
AATTATGATC AGAAGTCTCA GGAAGTTGAA GACAAAACAA AGGAATATGA ATTGCTTAGT 
GATGAATTGA ATCAAAAATC TGCAACTTTA GCAAGTATTG ATGCTGAGCT TCAGAAGCTG 
AAGGAAATGA CCAACCACCA GAAGAAACGA GCAGCTGAAA TGATGGCATC ATTACTAAAA 
GACCTTGCAG AAATAGGAAT TGCTGTGGGA AATAATGATG TAAAGCAACC GGAGGGGACT 
GGTATGATAG ATGAAGAGTT TACTGTTGCA AGGCTCTACA TTAGCAAAAT GAAATCAGAA 
GTAAAAACCA TGGTGAAACG CTGCAAACAG CTAGAAAGCA CACAGACTGA GAGCAACAAA 
AAAATGGAAG AAAATGAGAA GGAGTTAGCA GCATGCCAGC TTCGTATCTC CCAACATGAA 
GCCAAAATCA AGTCACTGAC TGAATACCTT CAAAATGTAG AACAAAAGAA GAGACAGCTG 
GAGGAATCTG TGGACTCCCT TGGTGAGGAG CTAGTCCAAC TTCGAGCACA AGAGAAAGTC 
CATGAAATGG AAAAAGAGCA CTTGAACAAG GTTCAGACTG CAAATGAAGT CAAGCAAGCT 
GTTGAACAGC AGATCCAGAG TCACAGAGAA ACCCACCAAA AACAAATCAG TAGTTTACGA 
GATGAAGTTG AGGCAAAGGA AAAGCTAATC ACTGATCTCC AAGACCAAAA CCAGAAGATG 
GTGTTGGAAC AGGAGCGGCT AAGGGTGGAG CATGAGAGGC TAAAGGCTGT AGACCAGGAG 
AAGAGCAGGA AGCTGCATGA GCTCACGGTT ATGCAAGACA GACGAGAACA AGCAAGGCAA 
GATTTGAAGG GGTTGGAAGA GACGGTGGCA AAAGAACTTC AGACTTTACA CAACCTGCGC 
AAGCTCTTTG TTCAGGACTT GGCTACCAGG GTGAAAAAGA GCGCTGAGGT GGACTCAGAC 
GACACTGGCG GCAGTGCTGC ACAGAAGCAG AAAATCTCCT TCCTTGAAAA CAATCTGGAG 
CAGCTCACCA AAGTGCACAA GCAGTTGGTG CGTGATAATG CAGATCTTCG CTGTGAACTT 
CCTAAGTTAG AGAAACGACT TAGAGCTACT GCAGAGAGAG TGAAAGCTTT GGAGTCAGCC 
CTGAAAGAAG CCAAAGAAAA TGCATCTCGA GACCGCAAAC GCTATCAGCA AGAAGTAGAC 
AGGATAAAGG AAGCAGTCAG GTCAAAGAAT ATGGCCAGAA GGGGACACTC TGCACAGATT 
GCAAAACCTA TCCGTCCTGG ACAGCATCCA GCAGCCTCTC CAACTCACCC CGGTGCAGTT 
CGTGGAGGAG GCTCATTTGT TCAGAACAAC CAGCCAGTGG GGCTTCGAGG TGGTGGAGGC 
AAGCAGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif5b ( XM_006254005.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254067.1 Rattus norvegicus kinesin family member 5B (Kif5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa11601
Clone ID Related Accession (Same CDS sequence)	NM_057202.1 , XM_006254005.1
Accession Version	XM_006254005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-1 heavy chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC CGGCGGAGTG CAACATCAAA GTGATGTGTC GCTTCAGACC TCTCAACGAG 
TCTGAAGTGA ACCGCGGCGA CAAGTATGTC GCCAAGTTCC AGGGAGAAGA CACGGTGATG 
ATTGCGTCCA AGCCTTATGC ATTTGATCGT GTGTTCCAGT CAAGCACATC TCAAGAGCAA 
GTATACAACG ATTGTGCAAA GAAGATTGTT AAAGATGTTC TTGAGGGCTA TAATGGAACA 
ATATTTGCAT ATGGACAAAC ATCATCTGGA AAGACCCACA CAATGGAGGG TAAACTTCAT 
GATCCAGAAG GCATGGGGAT TATTCCAAGA ATAGTGCAAG ATATTTTTAA TTATATTTAC 
TCCATGGATG AAAATTTGGA ATTTCATATT AAGGTTTCAT ATTTTGAAAT ATATTTGGAT 
AAGATAAGGG ACCTGTTAGA TGTTTCAAAG ACTAACCTTT CAGTCCATGA AGACAAAAAC 
CGTGTTCCCT ATGTAAAGGG GTGCACAGAG CGTTTTGTGT GTAGTCCAGA TGAAGTTATG 
GATACCATAG ATGAAGGGAA ATCCAACAGA CACGTAGCAG TTACAAATAT GAATGAACAT 
AGCTCTAGGA GTCACAGTAT ATTTCTTATC AATGTAAAGC AAGAGAATAC ACAGACAGAA 
CAGAAACTCA GTGGGAAGCT TTATCTGGTT GATTTAGCTG GAAGTGAAAA GGTTAGTAAA 
ACTGGAGCTG AGGGTGCCGT GCTGGATGAA GCTAAGAACA TCAACAAGTC ACTTTCTGCA 
CTTGGAAATG TCATTTCTGC TTTGGCAGAA GGCAGTACCT ATGTTCCTTA TCGAGACAGT 
AAAATGACCA GAATTCTTCA AGATTCATTA GGTGGCAACT GTAGGACCAC TATTGTCATT 
TGCTGCTCTC CGTCATCTTA CAATGAGTCT GAGACAAAGT CAACACTCCT CTTTGGTCAA 
AGGGCCAAAA CAATAAAGAA CACAGTCTGT GTCAACGTAG AGTTAACTGC AGAGCAGTGG 
AAAAAGAAGT ATGAAAAAGA AAAGGAAAAA AATAAGACTC TACGGAACAC CATTCAGTGG 
CTGGAAAACG AGCTCAACCG ATGGCGTAAC GGGGAAACAG TGCCTATTGA TGAGCAGTTT 
GACAAAGAGA AGGCTAATTT GGAAGCCTTC ACAGCAGATA AAGATGTTGC TATTACCAAT 
GACAAACCGG CTGCTGCAAT CGGAATGGCT GGTAGTTTTA CAGATGCTGA GAGAAGAAAG 
TGTGAAGAAG AAATTGCTAA ATTGTATAAA CAACTTGATG ACAAGGATGA AGAGATTAAC 
CAACAGAGCC AACTGGTGGA GAAATTGAAG ACACAAATGT TGGATCAGGA AGAGCTTCTG 
GCATCAACCA GAAGGGATCA AGATAATATG CAAGCTGAAC TGAATCGCCT CCAAGCAGAA 
AATGATGCTT CTAAAGAAGA AGTCAAAGAA GTTTTACAGG CCTTAGAGGA GCTGGCTGTT 
AATTATGATC AGAAGTCTCA GGAAGTTGAA GACAAAACAA AGGAATATGA ATTGCTTAGT 
GATGAATTGA ATCAAAAATC TGCAACTTTA GCAAGTATTG ATGCTGAGCT TCAGAAGCTG 
AAGGAAATGA CCAACCACCA GAAGAAACGA GCAGCTGAAA TGATGGCATC ATTACTAAAA 
GACCTTGCAG AAATAGGAAT TGCTGTGGGA AATAATGATG TAAAGCAACC GGAGGGGACT 
GGTATGATAG ATGAAGAGTT TACTGTTGCA AGGCTCTACA TTAGCAAAAT GAAATCAGAA 
GTAAAAACCA TGGTGAAACG CTGCAAACAG CTAGAAAGCA CACAGACTGA GAGCAACAAA 
AAAATGGAAG AAAATGAGAA GGAGTTAGCA GCATGCCAGC TTCGTATCTC CCAACATGAA 
GCCAAAATCA AGTCACTGAC TGAATACCTT CAAAATGTAG AACAAAAGAA GAGACAGCTG 
GAGGAATCTG TGGACTCCCT TGGTGAGGAG CTAGTCCAAC TTCGAGCACA AGAGAAAGTC 
CATGAAATGG AAAAAGAGCA CTTGAACAAG GTTCAGACTG CAAATGAAGT CAAGCAAGCT 
GTTGAACAGC AGATCCAGAG TCACAGAGAA ACCCACCAAA AACAAATCAG TAGTTTACGA 
GATGAAGTTG AGGCAAAGGA AAAGCTAATC ACTGATCTCC AAGACCAAAA CCAGAAGATG 
GTGTTGGAAC AGGAGCGGCT AAGGGTGGAG CATGAGAGGC TAAAGGCTGT AGACCAGGAG 
AAGAGCAGGA AGCTGCATGA GCTCACGGTT ATGCAAGACA GACGAGAACA AGCAAGGCAA 
GATTTGAAGG GGTTGGAAGA GACGGTGGCA AAAGAACTTC AGACTTTACA CAACCTGCGC 
AAGCTCTTTG TTCAGGACTT GGCTACCAGG GTGAAAAAGA GCGCTGAGGT GGACTCAGAC 
GACACTGGCG GCAGTGCTGC ACAGAAGCAG AAAATCTCCT TCCTTGAAAA CAATCTGGAG 
CAGCTCACCA AAGTGCACAA GCAGTTGGTG CGTGATAATG CAGATCTTCG CTGTGAACTT 
CCTAAGTTAG AGAAACGACT TAGAGCTACT GCAGAGAGAG TGAAAGCTTT GGAGTCAGCC 
CTGAAAGAAG CCAAAGAAAA TGCATCTCGA GACCGCAAAC GCTATCAGCA AGAAGTAGAC 
AGGATAAAGG AAGCAGTCAG GTCAAAGAAT ATGGCCAGAA GGGGACACTC TGCACAGATT 
GCAAAACCTA TCCGTCCTGG ACAGCATCCA GCAGCCTCTC CAACTCACCC CGGTGCAGTT 
CGTGGAGGAG GCTCATTTGT TCAGAACAAC CAGCCAGTGG GGCTTCGAGG TGGTGGAGGC 
AAGCAGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254067.1
CDS	432..3323
Translation	MADPAECNIKVMCRFRPLNESEVNRGDKYVAKFQGEDTVMIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKTLRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTADKDVAITNDKPAAAIGMAGSFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDSLGEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKEKLITDLQDQNQKMVLEQERLRVEHERLKAVDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEVDSDDTGGSAAQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKNMARRGHSAQIAKPIRPGQHPAASPTHPGAVRGGGSFVQNNQPVGLRGGGGKQA

Target ORF information:

RefSeq Version	XM_006254005.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 5B (Kif5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254005.1

ORF Insert Sequence:

ATGGCGGACC CGGCGGAGTG CAACATCAAA GTGATGTGTC GCTTCAGACC TCTCAACGAG 
TCTGAAGTGA ACCGCGGCGA CAAGTATGTC GCCAAGTTCC AGGGAGAAGA CACGGTGATG 
ATTGCGTCCA AGCCTTATGC ATTTGATCGT GTGTTCCAGT CAAGCACATC TCAAGAGCAA 
GTATACAACG ATTGTGCAAA GAAGATTGTT AAAGATGTTC TTGAGGGCTA TAATGGAACA 
ATATTTGCAT ATGGACAAAC ATCATCTGGA AAGACCCACA CAATGGAGGG TAAACTTCAT 
GATCCAGAAG GCATGGGGAT TATTCCAAGA ATAGTGCAAG ATATTTTTAA TTATATTTAC 
TCCATGGATG AAAATTTGGA ATTTCATATT AAGGTTTCAT ATTTTGAAAT ATATTTGGAT 
AAGATAAGGG ACCTGTTAGA TGTTTCAAAG ACTAACCTTT CAGTCCATGA AGACAAAAAC 
CGTGTTCCCT ATGTAAAGGG GTGCACAGAG CGTTTTGTGT GTAGTCCAGA TGAAGTTATG 
GATACCATAG ATGAAGGGAA ATCCAACAGA CACGTAGCAG TTACAAATAT GAATGAACAT 
AGCTCTAGGA GTCACAGTAT ATTTCTTATC AATGTAAAGC AAGAGAATAC ACAGACAGAA 
CAGAAACTCA GTGGGAAGCT TTATCTGGTT GATTTAGCTG GAAGTGAAAA GGTTAGTAAA 
ACTGGAGCTG AGGGTGCCGT GCTGGATGAA GCTAAGAACA TCAACAAGTC ACTTTCTGCA 
CTTGGAAATG TCATTTCTGC TTTGGCAGAA GGCAGTACCT ATGTTCCTTA TCGAGACAGT 
AAAATGACCA GAATTCTTCA AGATTCATTA GGTGGCAACT GTAGGACCAC TATTGTCATT 
TGCTGCTCTC CGTCATCTTA CAATGAGTCT GAGACAAAGT CAACACTCCT CTTTGGTCAA 
AGGGCCAAAA CAATAAAGAA CACAGTCTGT GTCAACGTAG AGTTAACTGC AGAGCAGTGG 
AAAAAGAAGT ATGAAAAAGA AAAGGAAAAA AATAAGACTC TACGGAACAC CATTCAGTGG 
CTGGAAAACG AGCTCAACCG ATGGCGTAAC GGGGAAACAG TGCCTATTGA TGAGCAGTTT 
GACAAAGAGA AGGCTAATTT GGAAGCCTTC ACAGCAGATA AAGATGTTGC TATTACCAAT 
GACAAACCGG CTGCTGCAAT CGGAATGGCT GGTAGTTTTA CAGATGCTGA GAGAAGAAAG 
TGTGAAGAAG AAATTGCTAA ATTGTATAAA CAACTTGATG ACAAGGATGA AGAGATTAAC 
CAACAGAGCC AACTGGTGGA GAAATTGAAG ACACAAATGT TGGATCAGGA AGAGCTTCTG 
GCATCAACCA GAAGGGATCA AGATAATATG CAAGCTGAAC TGAATCGCCT CCAAGCAGAA 
AATGATGCTT CTAAAGAAGA AGTCAAAGAA GTTTTACAGG CCTTAGAGGA GCTGGCTGTT 
AATTATGATC AGAAGTCTCA GGAAGTTGAA GACAAAACAA AGGAATATGA ATTGCTTAGT 
GATGAATTGA ATCAAAAATC TGCAACTTTA GCAAGTATTG ATGCTGAGCT TCAGAAGCTG 
AAGGAAATGA CCAACCACCA GAAGAAACGA GCAGCTGAAA TGATGGCATC ATTACTAAAA 
GACCTTGCAG AAATAGGAAT TGCTGTGGGA AATAATGATG TAAAGCAACC GGAGGGGACT 
GGTATGATAG ATGAAGAGTT TACTGTTGCA AGGCTCTACA TTAGCAAAAT GAAATCAGAA 
GTAAAAACCA TGGTGAAACG CTGCAAACAG CTAGAAAGCA CACAGACTGA GAGCAACAAA 
AAAATGGAAG AAAATGAGAA GGAGTTAGCA GCATGCCAGC TTCGTATCTC CCAACATGAA 
GCCAAAATCA AGTCACTGAC TGAATACCTT CAAAATGTAG AACAAAAGAA GAGACAGCTG 
GAGGAATCTG TGGACTCCCT TGGTGAGGAG CTAGTCCAAC TTCGAGCACA AGAGAAAGTC 
CATGAAATGG AAAAAGAGCA CTTGAACAAG GTTCAGACTG CAAATGAAGT CAAGCAAGCT 
GTTGAACAGC AGATCCAGAG TCACAGAGAA ACCCACCAAA AACAAATCAG TAGTTTACGA 
GATGAAGTTG AGGCAAAGGA AAAGCTAATC ACTGATCTCC AAGACCAAAA CCAGAAGATG 
GTGTTGGAAC AGGAGCGGCT AAGGGTGGAG CATGAGAGGC TAAAGGCTGT AGACCAGGAG 
AAGAGCAGGA AGCTGCATGA GCTCACGGTT ATGCAAGACA GACGAGAACA AGCAAGGCAA 
GATTTGAAGG GGTTGGAAGA GACGGTGGCA AAAGAACTTC AGACTTTACA CAACCTGCGC 
AAGCTCTTTG TTCAGGACTT GGCTACCAGG GTGAAAAAGA GCGCTGAGGT GGACTCAGAC 
GACACTGGCG GCAGTGCTGC ACAGAAGCAG AAAATCTCCT TCCTTGAAAA CAATCTGGAG 
CAGCTCACCA AAGTGCACAA GCAGTTGGTG CGTGATAATG CAGATCTTCG CTGTGAACTT 
CCTAAGTTAG AGAAACGACT TAGAGCTACT GCAGAGAGAG TGAAAGCTTT GGAGTCAGCC 
CTGAAAGAAG CCAAAGAAAA TGCATCTCGA GACCGCAAAC GCTATCAGCA AGAAGTAGAC 
AGGATAAAGG AAGCAGTCAG GTCAAAGAAT ATGGCCAGAA GGGGACACTC TGCACAGATT 
GCAAAACCTA TCCGTCCTGG ACAGCATCCA GCAGCCTCTC CAACTCACCC CGGTGCAGTT 
CGTGGAGGAG GCTCATTTGT TCAGAACAAC CAGCCAGTGG GGCTTCGAGG TGGTGGAGGC 
AAGCAGGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons.
Neuron94(2)347-362.e7(2017 Apr)
Gumy LF,Katrukha EA,Grigoriev I,Jaarsma D,Kapitein LC,Akhmanova A,Hoogenraad CC

Hypertrophy induced KIF5B controls mitochondrial localization and function in neonatal rat cardiomyocytes.
Journal of molecular and cellular cardiology9770-81(2016 Aug)
Tigchelaar W,de Jong AM,Bloks VW,van Gilst WH,de Boer RA,Sillj? HH

Kinesin 1 Drives Autolysosome Tubulation.
Developmental cell37(4)326-336(2016 05)
Du W,Su QP,Chen Y,Zhu Y,Jiang D,Rong Y,Zhang S,Zhang Y,Ren H,Zhang C,Wang X,Gao N,Wang Y,Sun L,Sun Y,Yu L

The Basic Domain of Herpes Simplex Virus 1 pUS9 Recruits Kinesin-1 To Facilitate Egress from Neurons.
Journal of virology90(4)2102-11(2015 Dec)
Diefenbach RJ,Davis A,Miranda-Saksena M,Fernandez MA,Kelly BJ,Jones CA,LaVail JH,Xue J,Lai J,Cunningham AL

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Kif5b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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