Kcnb2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnb2
Entrez Gene ID	117105
Full Name	potassium voltage-gated channel subfamily B member 2
Synonyms	Kv2.2
General protein information	Preferred Names potassium voltage-gated channel subfamily B member 2 Names potassium voltage-gated channel subfamily B member 2 CDRK potassium channel, voltage gated Shab-related subfamily B, member 2 potassium voltage gated channel, Shab-related subfamily, member 2 voltage-gated potassium channel subunit Kv2.2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q11
Summary	delayed rectifier potassium channel [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017593132.1	XP_017448621.1	potassium voltage-gated channel subfamily B member 2 isoform X1
NM_054000.2	NP_446452.2	potassium voltage-gated channel subfamily B member 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	KCNB2	NP_004761.2
Danio rerio (zebrafish)	LOC566883	XP_695259.4
	KCNB2	XP_001083422.1
Rattus norvegicus (Norway rat)	Kcnb2	NP_446452.2
Gallus gallus (chicken)	KCNB2	XP_003640873.1
Xenopus tropicalis (tropical clawed frog)	kcnb2	XP_002937117.2
Pan troglodytes (chimpanzee)	KCNB2	XP_528164.2
Canis lupus familiaris (dog)	KCNB2	NP_001003247.1
Bos taurus (cattle)	KCNB2	NP_001019734.1
Mus musculus (house mouse)	Kcnb2	NP_001091998.1

Gene Ontology
Bibliography

Related articles in PubMed

Distinct Cell- and Layer-Specific Expression Patterns and Independent Regulation of Kv2 Channel Subtypes in Cortical Pyramidal Neurons.
Bishop HI, Guan D, Bocksteins E, Parajuli LK, Murray KD, Cobb MM, Misonou H, Zito K, Foehring RC, Trimmer JS
The Journal of neuroscience : the official journal of the Society for Neuroscience35(44)14922-42(2015 Nov)

Kv2 dysfunction after peripheral axotomy enhances sensory neuron responsiveness to sustained input.
Tsantoulas C, Zhu L, Yip P, Grist J, Michael GJ, McMahon SB
Experimental neurology251115-26(2014 Jan)

Control of voltage-gated potassium channel Kv2.2 expression by pyruvate-isocitrate cycling regulates glucose-stimulated insulin secretion.
Jensen MV, Haldeman JM, Zhang H, Lu D, Huising MO, Vale WW, Hohmeier HE, Rosenberg P, Newgard CB
The Journal of biological chemistry288(32)23128-40(2013 Aug)

Immunolocalization of the voltage-gated potassium channel Kv2.2 in GABAergic neurons in the basal forebrain of rats and mice.
Hermanstyne TO, Kihira Y, Misono K, Deitchler A, Yanagawa Y, Misonou H
The Journal of comparative neurology518(21)4298-310(2010 Nov)

Formation of heteromeric Kv2 channels in mammalian brain neurons.
Kihira Y, Hermanstyne TO, Misonou H
The Journal of biological chemistry285(20)15048-55(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Kv2 family members, Kv2.1 and Kv2.2, are expresssed in distinct cortical layers and pyramidal cell types.
Title: Distinct Cell- and Layer-Specific Expression Patterns and Independent Regulation of Kv2 Channel Subtypes in Cortical Pyramidal Neurons.

From histological assessment illustrates that the Kv2.1 and Kv.2 subunits are widely expressed in a mixture of DRG neurons and appear enriched in the myelinated neuron population.
Title: Kv2 dysfunction after peripheral axotomy enhances sensory neuron responsiveness to sustained input.

a model in which a key function of the pyruvate-isocitrate cycle is to maintain levels of Kv2.2 expression sufficient to allow it to serve as a negative regulator of Kv channel activity.
Title: Control of voltage-gated potassium channel Kv2.2 expression by pyruvate-isocitrate cycling regulates glucose-stimulated insulin secretion.

These data offer Kv2.2 as a molecular target to study the role of the specific subpopulation of basal forebrain GABAergic neurons.
Title: Immunolocalization of the voltage-gated potassium channel Kv2.2 in GABAergic neurons in the basal forebrain of rats and mice.

Kv2.2 subunits make a minor contribution to slowly inactivating potassium currents in neocortical pyramidal neurons.
Title: Kv2 subunits underlie slowly inactivating potassium current in rat neocortical pyramidal neurons.

The following Kcnb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12080	NM_054000.2 Latest version!	Rattus norvegicus potassium voltage-gated channel subfamily B member 2 (Kcnb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa12080	XM_017593132.1 Latest version!	Rattus norvegicus potassium voltage-gated channel subfamily B member 2 (Kcnb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnb2 ( NM_054000.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446452.2 Rattus norvegicus potassium voltage-gated channel subfamily B member 2 (Kcnb2), mRNA.

Price & Availability↑

CloneID	ORa12080
Clone ID Related Accession (Same CDS sequence)	XM_017593132.1 , NM_054000.2
Accession Version	NM_054000.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	potassium voltage-gated channel subfamily B member 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473984.1. On Jan 12, 2008 this sequence version replaced NM_054000.1. ##Evidence-Data-START## Transcript exon combination :: M77482.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAAA AGGCACCTCC TGGCTTGAAC AGAAAAACAT CCAGGTCAAC ACTTTCTCTT 
CCTCCAGAAC CAGTGGACAT TATCCGAAGC AAAACATGCT CTCGGAGAGT TAAGATCAAC 
GTAGGGGGCC TTAACCACGA AGTCCTGTGG AGAACTCTGG ACAGGCTACC CAGGACGCGC 
CTGGGGAAGC TCCGAGACTG TAACACGCAC GAGAGTCTCC TGGAGGTGTG CGATGACTAC 
AACCTTAATG AGAATGAGTA TTTCTTCGAC CGCCATCCTG GAGCCTTCAC ATCTATTCTG 
AACTTCTACC GGACGGGAAA GCTCCACATG ATGGAAGAAA TGTGTGCCCT CTCCTTTGGC 
CAAGAGCTTG ATTACTGGGG CATCGATGAG ATCTACCTAG AGTCCTGCTG CCAAGCCAGA 
TACCATCAGA AGAAGGAGCA GATGAACGAA GAACTTAGAA GGGAGGCAGA GACCATGCGT 
GAGCGCGAGG GGGAGGAGTT TGATAACACC TGCTGCCCAG AGAAAAGGAA GAAACTTTGG 
GACTTGCTGG AGAAGCCGAA CTCATCCGTG GCGGCAAAGA TCCTGGCCAT CGTGTCTATC 
CTGTTCATCG TCCTTTCCAC CATTGCTCTG TCTCTCAACA CACTTCCGGA GCTGCAAGAA 
AACGATGAAT TTGGGCAACC CAGTGACAAC CGGAAGTTGG CGCACGTAGA GGCTGTGTGC 
ATTGCTTGGT TTACCATGGA GTACCTTTTA CGATTCCTGT CCTCACCAAA TAAATGGAAA 
TTCTTTAAAG GCCCACTGAA TGTCATTGAC CTCCTGGCCA TCTTGCCATA CTATGTAACC 
ATCTTCCTCA CCGAGTCCAA CAAAAGTGTG CTGCAGTTCC AGAATGTGAG ACGCGTGGTC 
CAGATCTTTC GAATCATGCG CATCCTCAGG ATCCTGAAAC TCGCCAGACA CTCGACGGGC 
CTGCAGTCTC TGGGTTTCAC CCTCAGGCGG AGTTACAATG AGTTGGGTTT ATTAATACTG 
TTTCTGGCCA TGGGAATAAT GATATTTTCC AGTTTGGTGT TTTTCGCTGA GAAAGATGAA 
GATGCTACAA AGTTCACCAG TATCCCTGCA TCATTTTGGT GGGCTACCAT CACCATGACC 
ACTGTGGGCT ATGGTGACAT TTACCCTAAA ACACTGTTAG GGAAAATTGT GGGTGGCCTT 
TGCTGCATTG CAGGAGTCCT GGTTATTGCC CTTCCTATCC CCATCATTGT GAACAATTTC 
TCTGAGTTCT ATAAGGAACA AAAACGCCAG GAGAAAGCTA TTAAAAGGAG AGAGGCTCTT 
GAAAGAGCCA AAAGAAATGG AAGCATCGTT TCTATGAACT TAAAGGATGC CTTTGCTAGA 
AGTATGGAAC TGATCGACGT GGCCGTGGAG AAGGCTGGAG AGTCGGCCAA TATCAAGGAC 
TCCGTGGATG ATAACCACCT GTCTCCAAGC CGGTGGAAGT GGGCCAGAAA GGCGCTGTCG 
GAAACAAGCT CCAACAAGTC TTACGAGAAT AAGTACCAGG AGGTTAGCCA AAAAGACTCC 
CACGAACAGC TGAACAACAC TTCTTCCTCC AGCCCACAGC ATCTGAGCGC CCAGAAGCTG 
GAGATGCTAT ACAATGAAAT CACCAAGACA CAGACTCATT CCCACCCGAA CCCAGACTGC 
CAAGAACAGC CAGAGAGGCC ATCTGCCTAT GAGGAGGAGA TAGAAATGGA AGAGGTGGTC 
TGCCCACAGG AGCAGCTGGC TGTGGCACAG ACCGAGGTCA TCGTGGACAT GAAGAGCACC 
TCCAGCATTG ACAGCTTCAC CAGCTGTGCC ACTGACTTCA CGGAGACTGA GAGGTCACCC 
CTGCCACCAC CCTCTGCCTC TCACTTGCAG ATGAAGTTCC CCACCGACCT CCCAGGAATG 
GATGAGCACC AAAGAGTCAG GGCACCTCCA TTCCTAACAC TAAGCAGAGA TAAGGGGCCT 
GCTGCCAGGG AGGCTGCACT GGATTATGCC CCAATTGACA TAACTGTGAA CCTCGATGCT 
GGGGCTTCCC ATGGTCCTTT GCAACCTGAC AGTGCCAGCG ACAGCCCCAA GAGCTCGCTG 
AAAGGGAGCA ACCCCCTCAA GTCTAGATCC CTAAAAGTGA ACTTTCAGGA AAACAGAGGC 
AGTGCACCCC AGACCCCACC CAGCACAGCC AGGCCACTGC CAGTAACCAC AGCTGACTTT 
CCGCTCACCA CCCCCCAGCA CATGAGTACC ATTCTTCTAG AAGAAGCCCT CCCCCAGGGA 
CAGAGACCCT TGCCGGGGGC TGATGTTTCA GCACACTGTC AGGGACCATC CAAAGGTCTA 
TCCCCACGAG TTCCCAAGCA AAAACTGTTT CCTTTCTCTT CTAGAGAAAG GAGGAGCTTC 
ACTGAAATAG ACACTGGAGA AGACGAAGAC TTCTTGGACC TCCAAAGGCC AAGACCAGAC 
AAGCAAGCAG ATCCCAGCCC CAACTGCTTA GCAGATAAGC CTGGAGAGGC CAGAGACCCT 
TTAAGAGAAG AGGGCTGTGT GGGTTCCTCC TCTCCCCAGA ACACAGACCA CAACTGTAGG 
CAAGACATTT ACCAGGCTGT GGGTGAAGTC AAAAAGGACA GTAGTCAAGA AGGGTACAAG 
ATGGAGAACC ACTTGTTTGC CCCAGAAATT CATTCTAACC CAGGAGACAC AGGTTATTGC 
CCCACTCGTG AGACCAGCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446452.2
CDS	324..3047
Translation	MAEKAPPGLNRKTSRSTLSLPPEPVDIIRSKTCSRRVKINVGGLNHEVLWRTLDRLPRTRLGKLRDCNTHESLLEVCDDYNLNENEYFFDRHPGAFTSILNFYRTGKLHMMEEMCALSFGQELDYWGIDEIYLESCCQARYHQKKEQMNEELRREAETMREREGEEFDNTCCPEKRKKLWDLLEKPNSSVAAKILAIVSILFIVLSTIALSLNTLPELQENDEFGQPSDNRKLAHVEAVCIAWFTMEYLLRFLSSPNKWKFFKGPLNVIDLLAILPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEDATKFTSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNLKDAFARSMELIDVAVEKAGESANIKDSVDDNHLSPSRWKWARKALSETSSNKSYENKYQEVSQKDSHEQLNNTSSSSPQHLSAQKLEMLYNEITKTQTHSHPNPDCQEQPERPSAYEEEIEMEEVVCPQEQLAVAQTEVIVDMKSTSSIDSFTSCATDFTETERSPLPPPSASHLQMKFPTDLPGMDEHQRVRAPPFLTLSRDKGPAAREAALDYAPIDITVNLDAGASHGPLQPDSASDSPKSSLKGSNPLKSRSLKVNFQENRGSAPQTPPSTARPLPVTTADFPLTTPQHMSTILLEEALPQGQRPLPGADVSAHCQGPSKGLSPRVPKQKLFPFSSRERRSFTEIDTGEDEDFLDLQRPRPDKQADPSPNCLADKPGEARDPLREEGCVGSSSPQNTDHNCRQDIYQAVGEVKKDSSQEGYKMENHLFAPEIHSNPGDTGYCPTRETSM

Target ORF information:

RefSeq Version	NM_054000.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium voltage-gated channel subfamily B member 2 (Kcnb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_054000.2

ORF Insert Sequence:

ATGGCAGAAA AGGCACCTCC TGGCTTGAAC AGAAAAACAT CCAGGTCAAC ACTTTCTCTT 
CCTCCAGAAC CAGTGGACAT TATCCGAAGC AAAACATGCT CTCGGAGAGT TAAGATCAAC 
GTAGGGGGCC TTAACCACGA AGTCCTGTGG AGAACTCTGG ACAGGCTACC CAGGACGCGC 
CTGGGGAAGC TCCGAGACTG TAACACGCAC GAGAGTCTCC TGGAGGTGTG CGATGACTAC 
AACCTTAATG AGAATGAGTA TTTCTTCGAC CGCCATCCTG GAGCCTTCAC ATCTATTCTG 
AACTTCTACC GGACGGGAAA GCTCCACATG ATGGAAGAAA TGTGTGCCCT CTCCTTTGGC 
CAAGAGCTTG ATTACTGGGG CATCGATGAG ATCTACCTAG AGTCCTGCTG CCAAGCCAGA 
TACCATCAGA AGAAGGAGCA GATGAACGAA GAACTTAGAA GGGAGGCAGA GACCATGCGT 
GAGCGCGAGG GGGAGGAGTT TGATAACACC TGCTGCCCAG AGAAAAGGAA GAAACTTTGG 
GACTTGCTGG AGAAGCCGAA CTCATCCGTG GCGGCAAAGA TCCTGGCCAT CGTGTCTATC 
CTGTTCATCG TCCTTTCCAC CATTGCTCTG TCTCTCAACA CACTTCCGGA GCTGCAAGAA 
AACGATGAAT TTGGGCAACC CAGTGACAAC CGGAAGTTGG CGCACGTAGA GGCTGTGTGC 
ATTGCTTGGT TTACCATGGA GTACCTTTTA CGATTCCTGT CCTCACCAAA TAAATGGAAA 
TTCTTTAAAG GCCCACTGAA TGTCATTGAC CTCCTGGCCA TCTTGCCATA CTATGTAACC 
ATCTTCCTCA CCGAGTCCAA CAAAAGTGTG CTGCAGTTCC AGAATGTGAG ACGCGTGGTC 
CAGATCTTTC GAATCATGCG CATCCTCAGG ATCCTGAAAC TCGCCAGACA CTCGACGGGC 
CTGCAGTCTC TGGGTTTCAC CCTCAGGCGG AGTTACAATG AGTTGGGTTT ATTAATACTG 
TTTCTGGCCA TGGGAATAAT GATATTTTCC AGTTTGGTGT TTTTCGCTGA GAAAGATGAA 
GATGCTACAA AGTTCACCAG TATCCCTGCA TCATTTTGGT GGGCTACCAT CACCATGACC 
ACTGTGGGCT ATGGTGACAT TTACCCTAAA ACACTGTTAG GGAAAATTGT GGGTGGCCTT 
TGCTGCATTG CAGGAGTCCT GGTTATTGCC CTTCCTATCC CCATCATTGT GAACAATTTC 
TCTGAGTTCT ATAAGGAACA AAAACGCCAG GAGAAAGCTA TTAAAAGGAG AGAGGCTCTT 
GAAAGAGCCA AAAGAAATGG AAGCATCGTT TCTATGAACT TAAAGGATGC CTTTGCTAGA 
AGTATGGAAC TGATCGACGT GGCCGTGGAG AAGGCTGGAG AGTCGGCCAA TATCAAGGAC 
TCCGTGGATG ATAACCACCT GTCTCCAAGC CGGTGGAAGT GGGCCAGAAA GGCGCTGTCG 
GAAACAAGCT CCAACAAGTC TTACGAGAAT AAGTACCAGG AGGTTAGCCA AAAAGACTCC 
CACGAACAGC TGAACAACAC TTCTTCCTCC AGCCCACAGC ATCTGAGCGC CCAGAAGCTG 
GAGATGCTAT ACAATGAAAT CACCAAGACA CAGACTCATT CCCACCCGAA CCCAGACTGC 
CAAGAACAGC CAGAGAGGCC ATCTGCCTAT GAGGAGGAGA TAGAAATGGA AGAGGTGGTC 
TGCCCACAGG AGCAGCTGGC TGTGGCACAG ACCGAGGTCA TCGTGGACAT GAAGAGCACC 
TCCAGCATTG ACAGCTTCAC CAGCTGTGCC ACTGACTTCA CGGAGACTGA GAGGTCACCC 
CTGCCACCAC CCTCTGCCTC TCACTTGCAG ATGAAGTTCC CCACCGACCT CCCAGGAATG 
GATGAGCACC AAAGAGTCAG GGCACCTCCA TTCCTAACAC TAAGCAGAGA TAAGGGGCCT 
GCTGCCAGGG AGGCTGCACT GGATTATGCC CCAATTGACA TAACTGTGAA CCTCGATGCT 
GGGGCTTCCC ATGGTCCTTT GCAACCTGAC AGTGCCAGCG ACAGCCCCAA GAGCTCGCTG 
AAAGGGAGCA ACCCCCTCAA GTCTAGATCC CTAAAAGTGA ACTTTCAGGA AAACAGAGGC 
AGTGCACCCC AGACCCCACC CAGCACAGCC AGGCCACTGC CAGTAACCAC AGCTGACTTT 
CCGCTCACCA CCCCCCAGCA CATGAGTACC ATTCTTCTAG AAGAAGCCCT CCCCCAGGGA 
CAGAGACCCT TGCCGGGGGC TGATGTTTCA GCACACTGTC AGGGACCATC CAAAGGTCTA 
TCCCCACGAG TTCCCAAGCA AAAACTGTTT CCTTTCTCTT CTAGAGAAAG GAGGAGCTTC 
ACTGAAATAG ACACTGGAGA AGACGAAGAC TTCTTGGACC TCCAAAGGCC AAGACCAGAC 
AAGCAAGCAG ATCCCAGCCC CAACTGCTTA GCAGATAAGC CTGGAGAGGC CAGAGACCCT 
TTAAGAGAAG AGGGCTGTGT GGGTTCCTCC TCTCCCCAGA ACACAGACCA CAACTGTAGG 
CAAGACATTT ACCAGGCTGT GGGTGAAGTC AAAAAGGACA GTAGTCAAGA AGGGTACAAG 
ATGGAGAACC ACTTGTTTGC CCCAGAAATT CATTCTAACC CAGGAGACAC AGGTTATTGC 
CCCACTCGTG AGACCAGCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnb2 ( XM_017593132.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448621.1 Rattus norvegicus potassium voltage-gated channel subfamily B member 2 (Kcnb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa12080
Clone ID Related Accession (Same CDS sequence)	XM_017593132.1 , NM_054000.2
Accession Version	XM_017593132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	potassium voltage-gated channel subfamily B member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAA AGGCACCTCC TGGCTTGAAC AGAAAAACAT CCAGGTCAAC ACTTTCTCTT 
CCTCCAGAAC CAGTGGACAT TATCCGAAGC AAAACATGCT CTCGGAGAGT TAAGATCAAC 
GTAGGGGGCC TTAACCACGA AGTCCTGTGG AGAACTCTGG ACAGGCTACC CAGGACGCGC 
CTGGGGAAGC TCCGAGACTG TAACACGCAC GAGAGTCTCC TGGAGGTGTG CGATGACTAC 
AACCTTAATG AGAATGAGTA TTTCTTCGAC CGCCATCCTG GAGCCTTCAC ATCTATTCTG 
AACTTCTACC GGACGGGAAA GCTCCACATG ATGGAAGAAA TGTGTGCCCT CTCCTTTGGC 
CAAGAGCTTG ATTACTGGGG CATCGATGAG ATCTACCTAG AGTCCTGCTG CCAAGCCAGA 
TACCATCAGA AGAAGGAGCA GATGAACGAA GAACTTAGAA GGGAGGCAGA GACCATGCGT 
GAGCGCGAGG GGGAGGAGTT TGATAACACC TGCTGCCCAG AGAAAAGGAA GAAACTTTGG 
GACTTGCTGG AGAAGCCGAA CTCATCCGTG GCGGCAAAGA TCCTGGCCAT CGTGTCTATC 
CTGTTCATCG TCCTTTCCAC CATTGCTCTG TCTCTCAACA CACTTCCGGA GCTGCAAGAA 
AACGATGAAT TTGGGCAACC CAGTGACAAC CGGAAGTTGG CGCACGTAGA GGCTGTGTGC 
ATTGCTTGGT TTACCATGGA GTACCTTTTA CGATTCCTGT CCTCACCAAA TAAATGGAAA 
TTCTTTAAAG GCCCACTGAA TGTCATTGAC CTCCTGGCCA TCTTGCCATA CTATGTAACC 
ATCTTCCTCA CCGAGTCCAA CAAAAGTGTG CTGCAGTTCC AGAATGTGAG ACGCGTGGTC 
CAGATCTTTC GAATCATGCG CATCCTCAGG ATCCTGAAAC TCGCCAGACA CTCGACGGGC 
CTGCAGTCTC TGGGTTTCAC CCTCAGGCGG AGTTACAATG AGTTGGGTTT ATTAATACTG 
TTTCTGGCCA TGGGAATAAT GATATTTTCC AGTTTGGTGT TTTTCGCTGA GAAAGATGAA 
GATGCTACAA AGTTCACCAG TATCCCTGCA TCATTTTGGT GGGCTACCAT CACCATGACC 
ACTGTGGGCT ATGGTGACAT TTACCCTAAA ACACTGTTAG GGAAAATTGT GGGTGGCCTT 
TGCTGCATTG CAGGAGTCCT GGTTATTGCC CTTCCTATCC CCATCATTGT GAACAATTTC 
TCTGAGTTCT ATAAGGAACA AAAACGCCAG GAGAAAGCTA TTAAAAGGAG AGAGGCTCTT 
GAAAGAGCCA AAAGAAATGG AAGCATCGTT TCTATGAACT TAAAGGATGC CTTTGCTAGA 
AGTATGGAAC TGATCGACGT GGCCGTGGAG AAGGCTGGAG AGTCGGCCAA TATCAAGGAC 
TCCGTGGATG ATAACCACCT GTCTCCAAGC CGGTGGAAGT GGGCCAGAAA GGCGCTGTCG 
GAAACAAGCT CCAACAAGTC TTACGAGAAT AAGTACCAGG AGGTTAGCCA AAAAGACTCC 
CACGAACAGC TGAACAACAC TTCTTCCTCC AGCCCACAGC ATCTGAGCGC CCAGAAGCTG 
GAGATGCTAT ACAATGAAAT CACCAAGACA CAGACTCATT CCCACCCGAA CCCAGACTGC 
CAAGAACAGC CAGAGAGGCC ATCTGCCTAT GAGGAGGAGA TAGAAATGGA AGAGGTGGTC 
TGCCCACAGG AGCAGCTGGC TGTGGCACAG ACCGAGGTCA TCGTGGACAT GAAGAGCACC 
TCCAGCATTG ACAGCTTCAC CAGCTGTGCC ACTGACTTCA CGGAGACTGA GAGGTCACCC 
CTGCCACCAC CCTCTGCCTC TCACTTGCAG ATGAAGTTCC CCACCGACCT CCCAGGAATG 
GATGAGCACC AAAGAGTCAG GGCACCTCCA TTCCTAACAC TAAGCAGAGA TAAGGGGCCT 
GCTGCCAGGG AGGCTGCACT GGATTATGCC CCAATTGACA TAACTGTGAA CCTCGATGCT 
GGGGCTTCCC ATGGTCCTTT GCAACCTGAC AGTGCCAGCG ACAGCCCCAA GAGCTCGCTG 
AAAGGGAGCA ACCCCCTCAA GTCTAGATCC CTAAAAGTGA ACTTTCAGGA AAACAGAGGC 
AGTGCACCCC AGACCCCACC CAGCACAGCC AGGCCACTGC CAGTAACCAC AGCTGACTTT 
CCGCTCACCA CCCCCCAGCA CATGAGTACC ATTCTTCTAG AAGAAGCCCT CCCCCAGGGA 
CAGAGACCCT TGCCGGGGGC TGATGTTTCA GCACACTGTC AGGGACCATC CAAAGGTCTA 
TCCCCACGAG TTCCCAAGCA AAAACTGTTT CCTTTCTCTT CTAGAGAAAG GAGGAGCTTC 
ACTGAAATAG ACACTGGAGA AGACGAAGAC TTCTTGGACC TCCAAAGGCC AAGACCAGAC 
AAGCAAGCAG ATCCCAGCCC CAACTGCTTA GCAGATAAGC CTGGAGAGGC CAGAGACCCT 
TTAAGAGAAG AGGGCTGTGT GGGTTCCTCC TCTCCCCAGA ACACAGACCA CAACTGTAGG 
CAAGACATTT ACCAGGCTGT GGGTGAAGTC AAAAAGGACA GTAGTCAAGA AGGGTACAAG 
ATGGAGAACC ACTTGTTTGC CCCAGAAATT CATTCTAACC CAGGAGACAC AGGTTATTGC 
CCCACTCGTG AGACCAGCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448621.1
CDS	764..3487
Translation	MAEKAPPGLNRKTSRSTLSLPPEPVDIIRSKTCSRRVKINVGGLNHEVLWRTLDRLPRTRLGKLRDCNTHESLLEVCDDYNLNENEYFFDRHPGAFTSILNFYRTGKLHMMEEMCALSFGQELDYWGIDEIYLESCCQARYHQKKEQMNEELRREAETMREREGEEFDNTCCPEKRKKLWDLLEKPNSSVAAKILAIVSILFIVLSTIALSLNTLPELQENDEFGQPSDNRKLAHVEAVCIAWFTMEYLLRFLSSPNKWKFFKGPLNVIDLLAILPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEDATKFTSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNLKDAFARSMELIDVAVEKAGESANIKDSVDDNHLSPSRWKWARKALSETSSNKSYENKYQEVSQKDSHEQLNNTSSSSPQHLSAQKLEMLYNEITKTQTHSHPNPDCQEQPERPSAYEEEIEMEEVVCPQEQLAVAQTEVIVDMKSTSSIDSFTSCATDFTETERSPLPPPSASHLQMKFPTDLPGMDEHQRVRAPPFLTLSRDKGPAAREAALDYAPIDITVNLDAGASHGPLQPDSASDSPKSSLKGSNPLKSRSLKVNFQENRGSAPQTPPSTARPLPVTTADFPLTTPQHMSTILLEEALPQGQRPLPGADVSAHCQGPSKGLSPRVPKQKLFPFSSRERRSFTEIDTGEDEDFLDLQRPRPDKQADPSPNCLADKPGEARDPLREEGCVGSSSPQNTDHNCRQDIYQAVGEVKKDSSQEGYKMENHLFAPEIHSNPGDTGYCPTRETSM

Target ORF information:

RefSeq Version	XM_017593132.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium voltage-gated channel subfamily B member 2 (Kcnb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593132.1

ORF Insert Sequence:

ATGGCAGAAA AGGCACCTCC TGGCTTGAAC AGAAAAACAT CCAGGTCAAC ACTTTCTCTT 
CCTCCAGAAC CAGTGGACAT TATCCGAAGC AAAACATGCT CTCGGAGAGT TAAGATCAAC 
GTAGGGGGCC TTAACCACGA AGTCCTGTGG AGAACTCTGG ACAGGCTACC CAGGACGCGC 
CTGGGGAAGC TCCGAGACTG TAACACGCAC GAGAGTCTCC TGGAGGTGTG CGATGACTAC 
AACCTTAATG AGAATGAGTA TTTCTTCGAC CGCCATCCTG GAGCCTTCAC ATCTATTCTG 
AACTTCTACC GGACGGGAAA GCTCCACATG ATGGAAGAAA TGTGTGCCCT CTCCTTTGGC 
CAAGAGCTTG ATTACTGGGG CATCGATGAG ATCTACCTAG AGTCCTGCTG CCAAGCCAGA 
TACCATCAGA AGAAGGAGCA GATGAACGAA GAACTTAGAA GGGAGGCAGA GACCATGCGT 
GAGCGCGAGG GGGAGGAGTT TGATAACACC TGCTGCCCAG AGAAAAGGAA GAAACTTTGG 
GACTTGCTGG AGAAGCCGAA CTCATCCGTG GCGGCAAAGA TCCTGGCCAT CGTGTCTATC 
CTGTTCATCG TCCTTTCCAC CATTGCTCTG TCTCTCAACA CACTTCCGGA GCTGCAAGAA 
AACGATGAAT TTGGGCAACC CAGTGACAAC CGGAAGTTGG CGCACGTAGA GGCTGTGTGC 
ATTGCTTGGT TTACCATGGA GTACCTTTTA CGATTCCTGT CCTCACCAAA TAAATGGAAA 
TTCTTTAAAG GCCCACTGAA TGTCATTGAC CTCCTGGCCA TCTTGCCATA CTATGTAACC 
ATCTTCCTCA CCGAGTCCAA CAAAAGTGTG CTGCAGTTCC AGAATGTGAG ACGCGTGGTC 
CAGATCTTTC GAATCATGCG CATCCTCAGG ATCCTGAAAC TCGCCAGACA CTCGACGGGC 
CTGCAGTCTC TGGGTTTCAC CCTCAGGCGG AGTTACAATG AGTTGGGTTT ATTAATACTG 
TTTCTGGCCA TGGGAATAAT GATATTTTCC AGTTTGGTGT TTTTCGCTGA GAAAGATGAA 
GATGCTACAA AGTTCACCAG TATCCCTGCA TCATTTTGGT GGGCTACCAT CACCATGACC 
ACTGTGGGCT ATGGTGACAT TTACCCTAAA ACACTGTTAG GGAAAATTGT GGGTGGCCTT 
TGCTGCATTG CAGGAGTCCT GGTTATTGCC CTTCCTATCC CCATCATTGT GAACAATTTC 
TCTGAGTTCT ATAAGGAACA AAAACGCCAG GAGAAAGCTA TTAAAAGGAG AGAGGCTCTT 
GAAAGAGCCA AAAGAAATGG AAGCATCGTT TCTATGAACT TAAAGGATGC CTTTGCTAGA 
AGTATGGAAC TGATCGACGT GGCCGTGGAG AAGGCTGGAG AGTCGGCCAA TATCAAGGAC 
TCCGTGGATG ATAACCACCT GTCTCCAAGC CGGTGGAAGT GGGCCAGAAA GGCGCTGTCG 
GAAACAAGCT CCAACAAGTC TTACGAGAAT AAGTACCAGG AGGTTAGCCA AAAAGACTCC 
CACGAACAGC TGAACAACAC TTCTTCCTCC AGCCCACAGC ATCTGAGCGC CCAGAAGCTG 
GAGATGCTAT ACAATGAAAT CACCAAGACA CAGACTCATT CCCACCCGAA CCCAGACTGC 
CAAGAACAGC CAGAGAGGCC ATCTGCCTAT GAGGAGGAGA TAGAAATGGA AGAGGTGGTC 
TGCCCACAGG AGCAGCTGGC TGTGGCACAG ACCGAGGTCA TCGTGGACAT GAAGAGCACC 
TCCAGCATTG ACAGCTTCAC CAGCTGTGCC ACTGACTTCA CGGAGACTGA GAGGTCACCC 
CTGCCACCAC CCTCTGCCTC TCACTTGCAG ATGAAGTTCC CCACCGACCT CCCAGGAATG 
GATGAGCACC AAAGAGTCAG GGCACCTCCA TTCCTAACAC TAAGCAGAGA TAAGGGGCCT 
GCTGCCAGGG AGGCTGCACT GGATTATGCC CCAATTGACA TAACTGTGAA CCTCGATGCT 
GGGGCTTCCC ATGGTCCTTT GCAACCTGAC AGTGCCAGCG ACAGCCCCAA GAGCTCGCTG 
AAAGGGAGCA ACCCCCTCAA GTCTAGATCC CTAAAAGTGA ACTTTCAGGA AAACAGAGGC 
AGTGCACCCC AGACCCCACC CAGCACAGCC AGGCCACTGC CAGTAACCAC AGCTGACTTT 
CCGCTCACCA CCCCCCAGCA CATGAGTACC ATTCTTCTAG AAGAAGCCCT CCCCCAGGGA 
CAGAGACCCT TGCCGGGGGC TGATGTTTCA GCACACTGTC AGGGACCATC CAAAGGTCTA 
TCCCCACGAG TTCCCAAGCA AAAACTGTTT CCTTTCTCTT CTAGAGAAAG GAGGAGCTTC 
ACTGAAATAG ACACTGGAGA AGACGAAGAC TTCTTGGACC TCCAAAGGCC AAGACCAGAC 
AAGCAAGCAG ATCCCAGCCC CAACTGCTTA GCAGATAAGC CTGGAGAGGC CAGAGACCCT 
TTAAGAGAAG AGGGCTGTGT GGGTTCCTCC TCTCCCCAGA ACACAGACCA CAACTGTAGG 
CAAGACATTT ACCAGGCTGT GGGTGAAGTC AAAAAGGACA GTAGTCAAGA AGGGTACAAG 
ATGGAGAACC ACTTGTTTGC CCCAGAAATT CATTCTAACC CAGGAGACAC AGGTTATTGC 
CCCACTCGTG AGACCAGCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distinct Cell- and Layer-Specific Expression Patterns and Independent Regulation of Kv2 Channel Subtypes in Cortical Pyramidal Neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience35(44)14922-42(2015 Nov)
Bishop HI,Guan D,Bocksteins E,Parajuli LK,Murray KD,Cobb MM,Misonou H,Zito K,Foehring RC,Trimmer JS

Kv2 dysfunction after peripheral axotomy enhances sensory neuron responsiveness to sustained input.
Experimental neurology251115-26(2014 Jan)
Tsantoulas C,Zhu L,Yip P,Grist J,Michael GJ,McMahon SB

Control of voltage-gated potassium channel Kv2.2 expression by pyruvate-isocitrate cycling regulates glucose-stimulated insulin secretion.
The Journal of biological chemistry288(32)23128-40(2013 Aug)
Jensen MV,Haldeman JM,Zhang H,Lu D,Huising MO,Vale WW,Hohmeier HE,Rosenberg P,Newgard CB

Immunolocalization of the voltage-gated potassium channel Kv2.2 in GABAergic neurons in the basal forebrain of rats and mice.
The Journal of comparative neurology518(21)4298-310(2010 Nov)
Hermanstyne TO,Kihira Y,Misono K,Deitchler A,Yanagawa Y,Misonou H

Formation of heteromeric Kv2 channels in mammalian brain neurons.
The Journal of biological chemistry285(20)15048-55(2010 May)
Kihira Y,Hermanstyne TO,Misonou H

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Kcnb2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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