gRNAs
The following Stim2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Stim2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa44109 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008770187.2 | |
| Accession Version | XM_008770187.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | stromal interaction molecule 2 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770187.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGGCATT TGGCCTTGAA TACAGGCACA CAGTGGAACT TACTGCCGTT GTTGACTAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008768409.1 |
| CDS | 6476..8620 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_008770187.2 |
1 | ATGGGGCATT TGGCCTTGAA TACAGGCACA CAGTGGAACT TACTGCCGTT GTTGACTAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa11311 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001105750.2 | |
| Accession Version | NM_001105750.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-04-30 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | stromal interaction molecule 2 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07015488.1 and AABR07015487.1. On May 31, 2013 this sequence version replaced NM_001105750.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. There are no full-length transcripts supporting this RefSeq in rat; however, it is predicted based on partial transcript alignments and on full-length transcript support from the mouse (GeneID: 116873, AK173183.1) and human (GeneID: 57620, AF328905.1) homologs. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689599 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGCTGCTCT TCGGGCTGTT GGTGGCCGGC GTGGCGGACG GATGCGACCT GGTGCCCCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001099220.2 |
| CDS | 214..2445 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001105750.2 |
1 | ATGCTGCTCT TCGGGCTGTT GGTGGCCGGC GTGGCGGACG GATGCGACCT GGTGCCCCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Inhibition of store-operated calcium entry by sub-lethal levels of proteasome inhibition is associated with STIM1/STIM2 degradation. |
STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs. |
Native STIM2 and ORAI1 proteins form a calcium-sensitive and thapsigargin-insensitive complex in cortical neurons. |
Canonical transient receptor potential channel 2 (TRPC2) as a major regulator of calcium homeostasis in rat thyroid FRTL-5 cells: importance of protein kinase C ? (PKC?) and stromal interaction molecule 2 (STIM2). |
STIM proteins: dynamic calcium signal transducers. |
