Rraga cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rraga
Entrez Gene ID	117044
Full Name	Ras-related GTP binding A
Synonyms	Raga
General protein information	Preferred Names Ras-related GTP binding A Names ras-related GTP-binding protein A Ras-related GTP-binding protein ragA rag A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q31
Summary	binds GTP; member of the subfamily of Ras-homologous GTP binding proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053973.2	NP_446425.1	ras-related GTP-binding protein A

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	gtr1	NP_595414.1
Bos taurus (cattle)	RRAGA	NP_001030576.1
Mus musculus (house mouse)	Rraga	NP_848463.1
Rattus norvegicus (Norway rat)	Rraga	NP_446425.1
Xenopus tropicalis (tropical clawed frog)	rraga	NP_001006897.1
Canis lupus familiaris (dog)	RRAGA	XP_003639405.2
Gallus gallus (chicken)	RRAGB	XP_420287.2
Danio rerio (zebrafish)	rraga	NP_956008.1
Homo sapiens (human)	RRAGA	NP_006561.1
Pan troglodytes (chimpanzee)	RRAGA	XP_528488.3
	RRAGA	XP_002800157.1

Gene Ontology
Bibliography

Related articles in PubMed

The ubiquitination of rag A GTPase by RNF152 negatively regulates mTORC1 activation.
Deng L, Jiang C, Chen L, Jin J, Wei J, Zhao L, Chen M, Pan W, Xu Y, Chu H, Wang X, Ge X, Li D, Liao L, Liu M, Li L, Wang P
Molecular cell58(5)804-18(2015 Jun)

Regulation of mTORC1 by amino acids.
Bar-Peled L, Sabatini DM
Trends in cell biology24(7)400-6(2014 Jul)

A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1.
Bar-Peled L, Chantranupong L, Cherniack AD, Chen WW, Ottina KA, Grabiner BC, Spear ED, Carter SL, Meyerson M, Sabatini DM
Science (New York, N.Y.)340(6136)1100-6(2013 May)

Leucyl-tRNA synthetase is an intracellular leucine sensor for the mTORC1-signaling pathway.
Han JM, Jeong SJ, Park MC, Kim G, Kwon NH, Kim HK, Ha SH, Ryu SH, Kim S
Cell149(2)410-24(2012 Apr)

Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids.
Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM
Cell141(2)290-303(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rraga gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rraga cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42609	NM_053973.2 Latest version!	Rattus norvegicus Ras-related GTP binding A (Rraga), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rraga ( NM_053973.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446425.1 Rattus norvegicus Ras-related GTP binding A (Rraga), mRNA.

Price & Availability↑

CloneID	ORa42609
Clone ID Related Accession (Same CDS sequence)	NM_053973.2
Accession Version	NM_053973.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	ras-related GTP-binding protein A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK599679.1, BC061850.1 and X85183.1. On Nov 23, 2005 this sequence version replaced NM_053973.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: X85183.1, BC061850.1 [ECO:0000345] ##Evidence-Data-END##

ATGCCAAATA CAGCCATGAA GAAAAAGGTG CTGTTGATGG GAAAGAGCGG GTCGGGGAAG 
ACCAGCATGA GGTCGATCAT CTTTGCAAAT TATATTGCGC GCGACACCAG GCGCCTGGGT 
GCCACCATCG ACGTGGAGCA CTCCCACGTC CGATTCTTGG GGAACCTGGT GCTGAACCTG 
TGGGACTGTG GCGGCCAGGA CACCTTTATG GAAAATTACT TCACCAGCCA GCGGGACAAC 
ATCTTCCGTA ATGTGGAGGT TCTGATCTAC GTGTTTGACG TAGAGAGCCG CGAACTGGAA 
AAAGATATGC ATTATTATCA GTCGTGTCTG GAAGCCATCC TCCAGAATTC ACCTGATGCC 
AAAATCTTCT GCCTGGTGCA CAAAATGGAT CTGGTTCAGG AAGATCAACG TGACCTGATT 
TTTAAAGAGC GAGAAGAAGA CCTGAGGCGT TTGTCTCGCC CGCTGGAGTG CGCTTGTTTT 
CGAACGTCCA TCTGGGATGA GACGCTCTAC AAAGCCTGGT CCAGCATCGT CTATCAGCTG 
ATCCCCAACG TTCAGCAGCT GGAGATGAAC CTAAGGAATT TTGCCCAGAT TATTGAGGCT 
GATGAAGTTC TGCTGTTCGA GAGAGCTACG TTCTTGGTGA TTTCCCACTA CCAGTGCAAA 
GAGCAGCGAG ATGTACACCG CTTTGAGAAG ATCAGCAACA TCATCAAGCA GTTTAAGCTA 
AGCTGTAGTA AATTGGCCGC TTCTTTCCAG AGCATGGAAG TGAGGAATTC TAACTTCGCT 
GCCTTCATCG ACATCTTTAC ATCAAACACG TATGTGATGG TGGTCATGTC AGATCCATCC 
ATCCCTTCTG CAGCTACTCT GATCAACATT CGTAATGCCA GGAAACACTT TGAGAAGCTA 
GAGAGAGTAG ATGGCCCCAA GCACAGCCTC CTCATGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446425.1
CDS	229..1170
Translation	MPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATIDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECACFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAQIIEADEVLLFERATFLVISHYQCKEQRDVHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKHSLLMR

Target ORF information:

RefSeq Version	NM_053973.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Ras-related GTP binding A (Rraga), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053973.2

ORF Insert Sequence:

ATGCCAAATA CAGCCATGAA GAAAAAGGTG CTGTTGATGG GAAAGAGCGG GTCGGGGAAG 
ACCAGCATGA GGTCGATCAT CTTTGCAAAT TATATTGCGC GCGACACCAG GCGCCTGGGT 
GCCACCATCG ACGTGGAGCA CTCCCACGTC CGATTCTTGG GGAACCTGGT GCTGAACCTG 
TGGGACTGTG GCGGCCAGGA CACCTTTATG GAAAATTACT TCACCAGCCA GCGGGACAAC 
ATCTTCCGTA ATGTGGAGGT TCTGATCTAC GTGTTTGACG TAGAGAGCCG CGAACTGGAA 
AAAGATATGC ATTATTATCA GTCGTGTCTG GAAGCCATCC TCCAGAATTC ACCTGATGCC 
AAAATCTTCT GCCTGGTGCA CAAAATGGAT CTGGTTCAGG AAGATCAACG TGACCTGATT 
TTTAAAGAGC GAGAAGAAGA CCTGAGGCGT TTGTCTCGCC CGCTGGAGTG CGCTTGTTTT 
CGAACGTCCA TCTGGGATGA GACGCTCTAC AAAGCCTGGT CCAGCATCGT CTATCAGCTG 
ATCCCCAACG TTCAGCAGCT GGAGATGAAC CTAAGGAATT TTGCCCAGAT TATTGAGGCT 
GATGAAGTTC TGCTGTTCGA GAGAGCTACG TTCTTGGTGA TTTCCCACTA CCAGTGCAAA 
GAGCAGCGAG ATGTACACCG CTTTGAGAAG ATCAGCAACA TCATCAAGCA GTTTAAGCTA 
AGCTGTAGTA AATTGGCCGC TTCTTTCCAG AGCATGGAAG TGAGGAATTC TAACTTCGCT 
GCCTTCATCG ACATCTTTAC ATCAAACACG TATGTGATGG TGGTCATGTC AGATCCATCC 
ATCCCTTCTG CAGCTACTCT GATCAACATT CGTAATGCCA GGAAACACTT TGAGAAGCTA 
GAGAGAGTAG ATGGCCCCAA GCACAGCCTC CTCATGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The ubiquitination of rag A GTPase by RNF152 negatively regulates mTORC1 activation.
Molecular cell58(5)804-18(2015 Jun)
Deng L,Jiang C,Chen L,Jin J,Wei J,Zhao L,Chen M,Pan W,Xu Y,Chu H,Wang X,Ge X,Li D,Liao L,Liu M,Li L,Wang P

Regulation of mTORC1 by amino acids.
Trends in cell biology24(7)400-6(2014 Jul)
Bar-Peled L,Sabatini DM

A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1.
Science (New York, N.Y.)340(6136)1100-6(2013 May)
Bar-Peled L,Chantranupong L,Cherniack AD,Chen WW,Ottina KA,Grabiner BC,Spear ED,Carter SL,Meyerson M,Sabatini DM

Leucyl-tRNA synthetase is an intracellular leucine sensor for the mTORC1-signaling pathway.
Cell149(2)410-24(2012 Apr)
Han JM,Jeong SJ,Park MC,Kim G,Kwon NH,Kim HK,Ha SH,Ryu SH,Kim S

Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids.
Cell141(2)290-303(2010 Apr)
Sancak Y,Bar-Peled L,Zoncu R,Markhard AL,Nada S,Sabatini DM

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Rraga cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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