RragB cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	RragB
Entrez Gene ID	117043
Full Name	Ras-related GTP binding B
Synonyms	Ragb
General protein information	Preferred Names Ras-related GTP binding B Names ras-related GTP-binding protein B GTP-binding protein ragB rag B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq12
Summary	short splice variant binds GTP [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006256775.3	XP_006256837.1	ras-related GTP-binding protein B isoform X1
XM_008773109.2	XP_008771331.2	ras-related GTP-binding protein B isoform X2
NM_053972.2	NP_446424.1	ras-related GTP-binding protein B

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	RragB	NP_446424.1
Rattus norvegicus (Norway rat)	LOC100909655	XP_003752070.1

Gene Ontology
Bibliography

Related articles in PubMed

A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1.
Bar-Peled L, Chantranupong L, Cherniack AD, Chen WW, Ottina KA, Grabiner BC, Spear ED, Carter SL, Meyerson M, Sabatini DM
Science (New York, N.Y.)340(6136)1100-6(2013 May)

SH3BP4 is a negative regulator of amino acid-Rag GTPase-mTORC1 signaling.
Kim YM, Stone M, Hwang TH, Kim YG, Dunlevy JR, Griffin TJ, Kim DH
Molecular cell46(6)833-46(2012 Jun)

Leucyl-tRNA synthetase is an intracellular leucine sensor for the mTORC1-signaling pathway.
Han JM, Jeong SJ, Park MC, Kim G, Kwon NH, Kim HK, Ha SH, Ryu SH, Kim S
Cell149(2)410-24(2012 Apr)

Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids.
Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM
Cell141(2)290-303(2010 Apr)

Molecular mechanisms of mTOR-mediated translational control.
Ma XM, Blenis J
Nature reviews. Molecular cell biology10(5)307-18(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RragB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RragB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11217	NM_053972.2 Latest version!	Rattus norvegicus Ras-related GTP binding B (RragB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa39633	XM_006256775.3 Latest version!	Rattus norvegicus Ras-related GTP binding B (RragB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa62993	XM_008773109.2 Latest version!	Rattus norvegicus Ras-related GTP binding B (RragB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RragB ( NM_053972.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446424.1 Rattus norvegicus Ras-related GTP binding B (RragB), mRNA.

Price & Availability↑

CloneID	ORa11217
Clone ID Related Accession (Same CDS sequence)	NM_053972.2
Accession Version	NM_053972.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	ras-related GTP-binding protein B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC087698.1. On Apr 5, 2007 this sequence version replaced NM_053972.1. ##Evidence-Data-START## Transcript exon combination :: BC078760.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGAAT CTGACTCCGA GAAAAAGACG GAGAAAGAGA ATGTGGGCCC AAAAGTGGAG 
CCTCCTCTAG GGGAGCCGGA AGGATCGCTT GGGTGGGCAA TGCCAAATGC AGCCATGAAG 
AAAAAGGTGC TGTTGATGGG TAAAAGTGGA TCTGGTAAGA CCAGCATGAG GTCTATTATC 
TTTGCAAATT ATATTGCCAG AGACACACGT CGTCTTGGTG CAACAATACT AGACCGTATA 
CATAGTCTTC AAATTAATAG CAGTTTGAGC ACCTACTCTC TCGTAGACTC TGTTGGAAAT 
ACAAAGACAT TTGATGTAGA ACATTCTCAT GTTCGATTTC TGGGAAACCT GGTTTTGAAC 
CTGTGGGATT GTGGTGGACA AGACACCTTC ATGGAAAATT ACTTCACTAG CCAGCGTGAC 
AACATCTTCC GAAATGTGGA GGTTCTGATT TATGTCTTTG ACGTGGAGAG CCGTGAACTC 
GAGAAAGACA TGCACTACTA TCAGTCATGC CTTGAGGCTA TTCTGCAGAA TTCCCCAGAA 
GCCAAAATCT TTTGCTTGGT ACACAAAATG GATTTGGTCC AGGAGGATCA GCGAGACTTG 
ATTTTTAAAG AACGAGAAGA AGACTTAAGG CGTTTATCGC GTCCGTTAGA ATGTTCTTGT 
TTCCGAACAT CTATCTGGGA TGAAACTCTG TATAAGGCTT GGTCCAGCAT TGTTTATCAG 
CTGATTCCCA ATGTTCAGCA ACTGGAAATG AACCTGCGCA ATTTTGCTGA AATTATTGAG 
GCTGATGAAG TACTTCTGTT TGAGAGAGCT ACCTTTCTGG TGATTTCTCA CTATCAGTGT 
AAAGAACAGC GAGATGCCCA CCGATTTGAG AAAATCAGCA ACATTATTAA GCAGTTCAAG 
CTGAGCTGTA GCAAGCTGGC TGCCTCTTTC CAGAGTATGG AAGTCAGGAA TTCTAACTTT 
GCTGCTTTCA TTGACATATT CACATCCAAC ACTTACGTCA TGGTCGTGAT GTCTGATCCA 
TCCATTCCTT CTGCAGCTAC TCTGATCAAC ATCCGCAATG CCAGAAAACA CTTTGAAAAG 
CTGGAGAGAG TGGATGGACC AAAACAGTGT CTTCTCATGC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446424.1
CDS	521..1645
Translation	MEESDSEKKTEKENVGPKVEPPLGEPEGSLGWAMPNAAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATILDRIHSLQINSSLSTYSLVDSVGNTKTFDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPEAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECSCFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAEIIEADEVLLFERATFLVISHYQCKEQRDAHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKQCLLMR

Target ORF information:

RefSeq Version	NM_053972.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Ras-related GTP binding B (RragB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053972.2

ORF Insert Sequence:

ATGGAAGAAT CTGACTCCGA GAAAAAGACG GAGAAAGAGA ATGTGGGCCC AAAAGTGGAG 
CCTCCTCTAG GGGAGCCGGA AGGATCGCTT GGGTGGGCAA TGCCAAATGC AGCCATGAAG 
AAAAAGGTGC TGTTGATGGG TAAAAGTGGA TCTGGTAAGA CCAGCATGAG GTCTATTATC 
TTTGCAAATT ATATTGCCAG AGACACACGT CGTCTTGGTG CAACAATACT AGACCGTATA 
CATAGTCTTC AAATTAATAG CAGTTTGAGC ACCTACTCTC TCGTAGACTC TGTTGGAAAT 
ACAAAGACAT TTGATGTAGA ACATTCTCAT GTTCGATTTC TGGGAAACCT GGTTTTGAAC 
CTGTGGGATT GTGGTGGACA AGACACCTTC ATGGAAAATT ACTTCACTAG CCAGCGTGAC 
AACATCTTCC GAAATGTGGA GGTTCTGATT TATGTCTTTG ACGTGGAGAG CCGTGAACTC 
GAGAAAGACA TGCACTACTA TCAGTCATGC CTTGAGGCTA TTCTGCAGAA TTCCCCAGAA 
GCCAAAATCT TTTGCTTGGT ACACAAAATG GATTTGGTCC AGGAGGATCA GCGAGACTTG 
ATTTTTAAAG AACGAGAAGA AGACTTAAGG CGTTTATCGC GTCCGTTAGA ATGTTCTTGT 
TTCCGAACAT CTATCTGGGA TGAAACTCTG TATAAGGCTT GGTCCAGCAT TGTTTATCAG 
CTGATTCCCA ATGTTCAGCA ACTGGAAATG AACCTGCGCA ATTTTGCTGA AATTATTGAG 
GCTGATGAAG TACTTCTGTT TGAGAGAGCT ACCTTTCTGG TGATTTCTCA CTATCAGTGT 
AAAGAACAGC GAGATGCCCA CCGATTTGAG AAAATCAGCA ACATTATTAA GCAGTTCAAG 
CTGAGCTGTA GCAAGCTGGC TGCCTCTTTC CAGAGTATGG AAGTCAGGAA TTCTAACTTT 
GCTGCTTTCA TTGACATATT CACATCCAAC ACTTACGTCA TGGTCGTGAT GTCTGATCCA 
TCCATTCCTT CTGCAGCTAC TCTGATCAAC ATCCGCAATG CCAGAAAACA CTTTGAAAAG 
CTGGAGAGAG TGGATGGACC AAAACAGTGT CTTCTCATGC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RragB ( XM_006256775.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256837.1 Rattus norvegicus Ras-related GTP binding B (RragB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39633
Clone ID Related Accession (Same CDS sequence)	XM_006256775.3
Accession Version	XM_006256775.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ras-related GTP-binding protein B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256775.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT CTGACTCCGA GAAAAAGACG GAGAAAGAGA ATGTGGGCCC AAAAGTGGAG 
CCTCCTCTAG GGGAGCCGGA AGGATCGCTT GGGTGGGCAA TGCCAAATGC AGCCATGAAG 
AAAAAGGTGC TGTTGATGGG TAAAAGTGGA TCTGGTAAGA CCAGCATGAG GTCTATTATC 
TTTGCAAATT ATATTGCCAG AGACACACGT CGTCTTGGTG CAACAATTGA TGTAGAACAT 
TCTCATGTTC GATTTCTGGG AAACCTGGTT TTGAACCTGT GGGATTGTGG TGGACAAGAC 
ACCTTCATGG AAAATTACTT CACTAGCCAG CGTGACAACA TCTTCCGAAA TGTGGAGGTT 
CTGATTTATG TCTTTGACGT GGAGAGCCGT GAACTCGAGA AAGACATGCA CTACTATCAG 
TCATGCCTTG AGGCTATTCT GCAGAATTCC CCAGAAGCCA AAATCTTTTG CTTGGTACAC 
AAAATGGATT TGGTCCAGGA GGATCAGCGA GACTTGATTT TTAAAGAACG AGAAGAAGAC 
TTAAGGCGTT TATCGCGTCC GTTAGAATGT TCTTGTTTCC GAACATCTAT CTGGGATGAA 
ACTCTGTATA AGGCTTGGTC CAGCATTGTT TATCAGCTGA TTCCCAATGT TCAGCAACTG 
GAAATGAACC TGCGCAATTT TGCTGAAATT ATTGAGGCTG ATGAAGTACT TCTGTTTGAG 
AGAGCTACCT TTCTGGTGAT TTCTCACTAT CAGTGTAAAG AACAGCGAGA TGCCCACCGA 
TTTGAGAAAA TCAGCAACAT TATTAAGCAG TTCAAGCTGA GCTGTAGCAA GCTGGCTGCC 
TCTTTCCAGA GTATGGAAGT CAGGAATTCT AACTTTGCTG CTTTCATTGA CATATTCACA 
TCCAACACTT ACGTCATGGT CGTGATGTCT GATCCATCCA TTCCTTCTGC AGCTACTCTG 
ATCAACATCC GCAATGCCAG AAAACACTTT GAAAAGCTGG AGAGAGTGGA TGGACCAAAA 
CAGTGTCTTC TCATGCGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256837.1
CDS	683..1723
Translation	MEESDSEKKTEKENVGPKVEPPLGEPEGSLGWAMPNAAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATIDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPEAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECSCFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAEIIEADEVLLFERATFLVISHYQCKEQRDAHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKQCLLMR

Target ORF information:

RefSeq Version	XM_006256775.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Ras-related GTP binding B (RragB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256775.3

ORF Insert Sequence:

ATGGAAGAAT CTGACTCCGA GAAAAAGACG GAGAAAGAGA ATGTGGGCCC AAAAGTGGAG 
CCTCCTCTAG GGGAGCCGGA AGGATCGCTT GGGTGGGCAA TGCCAAATGC AGCCATGAAG 
AAAAAGGTGC TGTTGATGGG TAAAAGTGGA TCTGGTAAGA CCAGCATGAG GTCTATTATC 
TTTGCAAATT ATATTGCCAG AGACACACGT CGTCTTGGTG CAACAATTGA TGTAGAACAT 
TCTCATGTTC GATTTCTGGG AAACCTGGTT TTGAACCTGT GGGATTGTGG TGGACAAGAC 
ACCTTCATGG AAAATTACTT CACTAGCCAG CGTGACAACA TCTTCCGAAA TGTGGAGGTT 
CTGATTTATG TCTTTGACGT GGAGAGCCGT GAACTCGAGA AAGACATGCA CTACTATCAG 
TCATGCCTTG AGGCTATTCT GCAGAATTCC CCAGAAGCCA AAATCTTTTG CTTGGTACAC 
AAAATGGATT TGGTCCAGGA GGATCAGCGA GACTTGATTT TTAAAGAACG AGAAGAAGAC 
TTAAGGCGTT TATCGCGTCC GTTAGAATGT TCTTGTTTCC GAACATCTAT CTGGGATGAA 
ACTCTGTATA AGGCTTGGTC CAGCATTGTT TATCAGCTGA TTCCCAATGT TCAGCAACTG 
GAAATGAACC TGCGCAATTT TGCTGAAATT ATTGAGGCTG ATGAAGTACT TCTGTTTGAG 
AGAGCTACCT TTCTGGTGAT TTCTCACTAT CAGTGTAAAG AACAGCGAGA TGCCCACCGA 
TTTGAGAAAA TCAGCAACAT TATTAAGCAG TTCAAGCTGA GCTGTAGCAA GCTGGCTGCC 
TCTTTCCAGA GTATGGAAGT CAGGAATTCT AACTTTGCTG CTTTCATTGA CATATTCACA 
TCCAACACTT ACGTCATGGT CGTGATGTCT GATCCATCCA TTCCTTCTGC AGCTACTCTG 
ATCAACATCC GCAATGCCAG AAAACACTTT GAAAAGCTGG AGAGAGTGGA TGGACCAAAA 
CAGTGTCTTC TCATGCGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RragB ( XM_008773109.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771331.2 Rattus norvegicus Ras-related GTP binding B (RragB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa62993
Clone ID Related Accession (Same CDS sequence)	XM_008773109.2
Accession Version	XM_008773109.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ras-related GTP-binding protein B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773109.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT CTGACTCCGA GAAAAAGACG GAGAAAGAGA ATGTGGGCCC AAAAGTGGAG 
CCTCCTCTAG GGGAGCCGGA AGGATCGCTT GGGTGGGCAA TGCCAAATGC AGCCATGAAG 
AAAAAGGTGC TGTTGATGGG TAAAAGTGGA TCTGGTAAGA CCAGCATGAG GTCTATTATC 
TTTGCAAATT ATATTGCCAG AGACACACGT CGTCTTGGTG CAACAATACT AGACCGTATA 
CATAGTCTTC AAATTAATAG CAGTTTGAGC ACCTACTCTC TCGTAGACTC TGTTGGAAAT 
ACAAAGACAT TTGATGTAGA ACATTCTCAT GTTCGATTTC TGGGAAACCT GGTTTTGAAC 
CTGTGGGATT GTGGTGGACA AGACACCTTC ATGGAAAATT ACTTCACTAG CCAGCGTGAC 
AACATCTTCC GAAATGTGGA GGTTCTGATT TATGTCTTTG ACGTGGAGAG CCGTGAACTC 
GAGAAAGACA TGCACTACTA TCAGTCATGC CTTGAGGCTA TTCTGCAGAA TTCCCCAGAA 
GCCAAAATCT TTTGCTTGGT ACACAAAATG GATTTGGTCC AGGAGGATCA GCGAGACTTG 
ATTTTTAAAG AACGAGAAGA AGACTTAAGG CGTTTATCGC GTCCGTTAGA ATGTTCTTGT 
TTCCGAACAT CTATCTGGGA TGAAACTCTG TATAAGGCTT GGTCCAGCAT TGTTTATCAG 
CTGATTCCCA ATGTTCAGCA ACTGGAAATG AACCTGCGCA ATTTTGCTGA AATTATTGAG 
GCTGATGAAG TACTTCTGTT TGAGAGAGCT ACCTTTCTGG TGATTTCTCA CTATCAGTGT 
AAAGAACAGC GAGATGCCCA CCGATTTGAG AAAATCAGCA ACATTATTAA GCAGTTCAAG 
CTGAGCTGTA GCAAGCTGGC TGCCTCTTTC CAGAGTATGG AAGTCAGGAA TTCTAACTTT 
GCTGCTTTCA TTGACATATT CACATCCAAC ACTTACGTCA TGGTCGTGAT GTCTGATCCA 
TCCATTCCCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771331.2
CDS	685..1719
Translation	MEESDSEKKTEKENVGPKVEPPLGEPEGSLGWAMPNAAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATILDRIHSLQINSSLSTYSLVDSVGNTKTFDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPEAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECSCFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAEIIEADEVLLFERATFLVISHYQCKEQRDAHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPF

Target ORF information:

RefSeq Version	XM_008773109.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Ras-related GTP binding B (RragB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773109.2

ORF Insert Sequence:

ATGGAAGAAT CTGACTCCGA GAAAAAGACG GAGAAAGAGA ATGTGGGCCC AAAAGTGGAG 
CCTCCTCTAG GGGAGCCGGA AGGATCGCTT GGGTGGGCAA TGCCAAATGC AGCCATGAAG 
AAAAAGGTGC TGTTGATGGG TAAAAGTGGA TCTGGTAAGA CCAGCATGAG GTCTATTATC 
TTTGCAAATT ATATTGCCAG AGACACACGT CGTCTTGGTG CAACAATACT AGACCGTATA 
CATAGTCTTC AAATTAATAG CAGTTTGAGC ACCTACTCTC TCGTAGACTC TGTTGGAAAT 
ACAAAGACAT TTGATGTAGA ACATTCTCAT GTTCGATTTC TGGGAAACCT GGTTTTGAAC 
CTGTGGGATT GTGGTGGACA AGACACCTTC ATGGAAAATT ACTTCACTAG CCAGCGTGAC 
AACATCTTCC GAAATGTGGA GGTTCTGATT TATGTCTTTG ACGTGGAGAG CCGTGAACTC 
GAGAAAGACA TGCACTACTA TCAGTCATGC CTTGAGGCTA TTCTGCAGAA TTCCCCAGAA 
GCCAAAATCT TTTGCTTGGT ACACAAAATG GATTTGGTCC AGGAGGATCA GCGAGACTTG 
ATTTTTAAAG AACGAGAAGA AGACTTAAGG CGTTTATCGC GTCCGTTAGA ATGTTCTTGT 
TTCCGAACAT CTATCTGGGA TGAAACTCTG TATAAGGCTT GGTCCAGCAT TGTTTATCAG 
CTGATTCCCA ATGTTCAGCA ACTGGAAATG AACCTGCGCA ATTTTGCTGA AATTATTGAG 
GCTGATGAAG TACTTCTGTT TGAGAGAGCT ACCTTTCTGG TGATTTCTCA CTATCAGTGT 
AAAGAACAGC GAGATGCCCA CCGATTTGAG AAAATCAGCA ACATTATTAA GCAGTTCAAG 
CTGAGCTGTA GCAAGCTGGC TGCCTCTTTC CAGAGTATGG AAGTCAGGAA TTCTAACTTT 
GCTGCTTTCA TTGACATATT CACATCCAAC ACTTACGTCA TGGTCGTGAT GTCTGATCCA 
TCCATTCCCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1.
Science (New York, N.Y.)340(6136)1100-6(2013 May)
Bar-Peled L,Chantranupong L,Cherniack AD,Chen WW,Ottina KA,Grabiner BC,Spear ED,Carter SL,Meyerson M,Sabatini DM

SH3BP4 is a negative regulator of amino acid-Rag GTPase-mTORC1 signaling.
Molecular cell46(6)833-46(2012 Jun)
Kim YM,Stone M,Hwang TH,Kim YG,Dunlevy JR,Griffin TJ,Kim DH

Leucyl-tRNA synthetase is an intracellular leucine sensor for the mTORC1-signaling pathway.
Cell149(2)410-24(2012 Apr)
Han JM,Jeong SJ,Park MC,Kim G,Kwon NH,Kim HK,Ha SH,Ryu SH,Kim S

Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids.
Cell141(2)290-303(2010 Apr)
Sancak Y,Bar-Peled L,Zoncu R,Markhard AL,Nada S,Sabatini DM

Molecular mechanisms of mTOR-mediated translational control.
Nature reviews. Molecular cell biology10(5)307-18(2009 May)
Ma XM,Blenis J

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RragB cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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