Nln cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nln
Entrez Gene ID	117041
Full Name	neurolysin
General protein information	Preferred Names neurolysin Names neurolysin, mitochondrial MEP microsomal endopeptidase mitochondrial oligopeptidase M neurolysin (metallopeptidase M3 family) neurotensin endopeptidase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	endopeptidase that cleaves bioactive and synthetic peptides [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231880.3	XP_006231942.1	neurolysin, mitochondrial isoform X1
XM_017590591.1	XP_017446080.1	neurolysin, mitochondrial isoform X2
NM_053970.2	NP_446422.2	neurolysin, mitochondrial precursor
XM_006231881.2	XP_006231943.1	neurolysin, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NLN	XP_001163138.1
Rattus norvegicus (Norway rat)	Nln	NP_446422.2
Magnaporthe oryzae (rice blast fungus)	MGG_04858	XP_003712337.1
Mus musculus (house mouse)	Nln	NP_083723.1
	LOC696884	XP_001087239.1
Kluyveromyces lactis	KLLA0C00572g	XP_452219.1
Neurospora crassa	NCU06618	XP_962360.1
Homo sapiens (human)	NLN	NP_065777.1
Canis lupus familiaris (dog)	NLN	XP_005617505.1
Bos taurus (cattle)	NLN	NP_001029161.2
Saccharomyces cerevisiae (baker's yeast)	PRD1	NP_009874.1
Xenopus tropicalis (tropical clawed frog)	nln	XP_002934236.2
Gallus gallus (chicken)	NLN	XP_424755.2

Gene Ontology
Bibliography

Related articles in PubMed

Allosteric inhibition of the neuropeptidase neurolysin.
Hines CS, Ray K, Schmidt JJ, Xiong F, Feenstra RW, Pras-Raves M, de Moes JP, Lange JH, Melikishvili M, Fried MG, Mortenson P, Charlton M, Patel Y, Courtney SM, Kruse CG, Rodgers DW
The Journal of biological chemistry289(51)35605-19(2014 Dec)

Hepatic scavenger receptor BI protects against polymicrobial-induced sepsis through promoting LPS clearance in mice.
Guo L, Zheng Z, Ai J, Huang B, Li XA
The Journal of biological chemistry289(21)14666-73(2014 May)

Neurolysin knockout mice generation and initial phenotype characterization.
Cavalcanti DM, Castro LM, Rosa Neto JC, Seelaender M, Neves RX, Oliveira V, Forti FL, Iwai LK, Gozzo FC, Todiras M, Schadock I, Barros CC, Bader M, Ferro ES
The Journal of biological chemistry289(22)15426-40(2014 May)

Pharmacological characterization of a novel non-AT1, non-AT2 angiotensin binding site identified as neurolysin.
Swindle JD, Santos KL, Speth RC
Endocrine44(2)525-31(2013 Oct)

Identification of membrane-bound variant of metalloendopeptidase neurolysin (EC 3.4.24.16) as the non-angiotensin type 1 (non-AT1), non-AT2 angiotensin binding site.
Wangler NJ, Santos KL, Schadock I, Hagen FK, Escher E, Bader M, Speth RC, Karamyan VT
The Journal of biological chemistry287(1)114-22(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that the inhibitor binds away from the catalytic site of neuropeptidase neurolysin.
Title: Allosteric inhibition of the neuropeptidase neurolysin.

Neurolysin was identified as a non-AT1 and non-AT2 binding site for Angiotensin II, suggesting that neurolysin may significantly affect the activity of the renin-angiotensin system.
Title: Pharmacological characterization of a novel non-AT1, non-AT2 angiotensin binding site identified as neurolysin.

Ciliary epithelium expresses neurolysin.
Title: Molecular analysis of neurolysin expression in the rat and bovine ciliary body.

Taken together, these data demonstrate that EP24.15/16 modulate intracellular, peptidergic signaling cascades through B2R in a physiologically relevant nociceptive system.
Title: Modulation of bradykinin signaling by EP24.15 and EP24.16 in cultured trigeminal ganglia.

EP24.16 activity in vascular endothelial cells may play an important role in the degradation of bradykinin and/or other peptides in the circulation
Title: Endopeptidases 3.4.24.15 and 24.16 in endothelial cells: potential role in vasoactive peptide metabolism.

The following Nln gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nln cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12841	NM_053970.2 Latest version!	Rattus norvegicus neurolysin (Nln), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa24362	XM_006231880.3 Latest version!	Rattus norvegicus neurolysin (Nln), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa24363	XM_017590591.1 Latest version!	Rattus norvegicus neurolysin (Nln), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa24363	XM_006231881.2 Latest version!	Rattus norvegicus neurolysin (Nln), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nln ( NM_053970.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446422.2 Rattus norvegicus neurolysin (Nln), mRNA.

Price & Availability↑

CloneID	ORa12841
Clone ID Related Accession (Same CDS sequence)	NM_053970.2
Accession Version	NM_053970.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	neurolysin, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC072687.1. On Jul 8, 2004 this sequence version replaced NM_053970.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC072687.1, X87157.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGATCACCC TGTGCCTTTC GACTCTGCGA GGCCTCCACA GAGCTGGTGG CTCCAGGCTT 
CAGCTGACAA TGACACTGGG AAAGGAGCTG GCTTCTCCTC TTCAAGCTAT GTCTTCCTAC 
ACTGCGGCTG GCAGGAATGT TCTCAGATGG GACCTTTCTC CAGAGCAGAT CAAGACGAGG 
ACAGAGCAGC TCATAGCACA GACCAAGCAG GTGTACGACA CCGTGGGGAC AATCGCCCTG 
AAAGAAGTAA CTTATGAGAA CTGTCTGCAG GTGCTGGCTG ACATAGAAGT GACGTACATA 
GTGGAAAGGA CCATGCTGGA CTTCCCTCAG CACGTGTCAT CTGACAGAGA AGTGCGGGCT 
GCAAGCACAG AAGCAGACAA AAGGCTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGAA 
GATGTGTTTC AGAGAATTGT TCATTTACAA GAAACATGCG ATTTGGAGAA GATAAAGCCT 
GAAGCCAGGC GATACTTGGA AAAGTCAATT AAAATGGGAA AAAGGAATGG ACTCCATCTT 
TCAGAACACA TAAGAAATGA AATCAAATCA ATGAAGAAGA GAATGAGTGA GCTATGCATT 
GACTTCAACA AGAACCTCAA TGAGGACGAC ACTTCCCTTG TATTCTCCAA GGCTGAACTT 
GGTGCTCTTC CTGATGACTT CATTGACAGT TTAGAAAAAA CAGATGAAGA CAAGTACAAA 
GTTACCTTAA AGTATCCTCA CTACTTCCCT GTCATGAAGA AATGCTGTGT CCCTGAAACT 
CGAAGGAAGA TGGAAATGGC TTTTCATACC AGGTGCAAAC AGGAAAACAC CGCAATTCTG 
CAACAGCTCC TCCCGCTGCG AGCTCAAGTG GCCAAGCTGC TGGGCTATAA CACCCATGCT 
GACTTTGTCC TTGAACTGAA CACTGCGAAG AGCACCAGCC GCGTGGCCGC CTTTCTAGAT 
GATTTAAGCC AGAAATTGAA ACCCTTGGGT GAGGCAGAAC GGGAGTTTAT TTTGAGCCTG 
AAGAAGAAGG AATGTGAAGA GCGAGGTTTT GAATACGACG GGAAGATCAA TGCATGGGAC 
CTCCATTACT ACATGACACA GACAGAGGAG CTCAAGTACT CGGTGGACCA GGAGTCCCTT 
AAGGAGTACT TCCCCATTGA GGTGGTCACG GAAGGCTTGC TAAGCATCTA CCAGGAGCTG 
CTGGGACTTT CATTTGAACA AGTGCCCGAT GCTCATGTTT GGAATAAGAG CGTTTCACTT 
TACACTGTGA AGGATAAAGC TACAGGAGAG GTCCTGGGGC AGTTCTACCT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA CCATGCAGCC TGCTTCGGTC TCCAGCCAGG CTGCCTTCTC 
CCTGATGGCA GTCGGATGAT GTCTGTGGCC GCCCTGGTGG TCAACTTCTC TCAGCCTGTA 
GCAGGTCGGC CCTCTCTCCT GAGACATGAT GAAGTGCGGA CTTACTTCCA CGAGTTCGGT 
CACGTCATGC ATCAGATCTG TGCGCAGACT GACTTTGCAC GATTCAGTGG AACAAACGTG 
GAAACTGACT TTGTAGAGGT GCCATCACAA ATGCTTGAAA ACTGGGTGTG GGACGTTGAT 
TCCCTGCGAA AACTGTCAAA GCATTATAAA GATGGACACC CTATCACAGA CGAGCTGCTC 
GAGAAGCTTG TTGCTTCTAG GCTGGTCAAC ACAGGTCTTC TGACGCTGCG CCAAATTGTT 
TTGAGCAAAG TTGACCAATC TCTCCATACC AATGCGACTC TGGATGCCGC GAGCGAATAC 
GCTAAATACT GCACAGAAAT TCTGGGAGTT GCAGCTACGC CAGGCACAAA TATGCCAGCT 
ACTTTTGGAC ACTTGGCAGG AGGGTATGAT GGCCAATATT ATGGATATCT TTGGAGTGAA 
GTGTTTTCCA TGGACATGTT TCACAGCTGT TTTAAAAAAG AGGGGATCAT GAATCCAGAG 
GTTGGAATGA AATACAGAAA CTTAATCCTG AAGCCTGGGG GGTCCCTGGA CGGCATGGAC 
ATGCTCCAGA ATTTCTTGCA ACGTGAGCCA AACCAAAAAG CATTCCTAAT GAGTCGAGGC 
CTGAATGGTT CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446422.2
CDS	36..2150
Translation	MITLCLSTLRGLHRAGGSRLQLTMTLGKELASPLQAMSSYTAAGRNVLRWDLSPEQIKTRTEQLIAQTKQVYDTVGTIALKEVTYENCLQVLADIEVTYIVERTMLDFPQHVSSDREVRAASTEADKRLSRFDIEMSMREDVFQRIVHLQETCDLEKIKPEARRYLEKSIKMGKRNGLHLSEHIRNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKAELGALPDDFIDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKQENTAILQQLLPLRAQVAKLLGYNTHADFVLELNTAKSTSRVAAFLDDLSQKLKPLGEAEREFILSLKKKECEERGFEYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQVPDAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMSVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRKLSKHYKDGHPITDELLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNATLDAASEYAKYCTEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFHSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLQREPNQKAFLMSRGLNGS

Target ORF information:

RefSeq Version	NM_053970.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurolysin (Nln), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053970.2

ORF Insert Sequence:

ATGATCACCC TGTGCCTTTC GACTCTGCGA GGCCTCCACA GAGCTGGTGG CTCCAGGCTT 
CAGCTGACAA TGACACTGGG AAAGGAGCTG GCTTCTCCTC TTCAAGCTAT GTCTTCCTAC 
ACTGCGGCTG GCAGGAATGT TCTCAGATGG GACCTTTCTC CAGAGCAGAT CAAGACGAGG 
ACAGAGCAGC TCATAGCACA GACCAAGCAG GTGTACGACA CCGTGGGGAC AATCGCCCTG 
AAAGAAGTAA CTTATGAGAA CTGTCTGCAG GTGCTGGCTG ACATAGAAGT GACGTACATA 
GTGGAAAGGA CCATGCTGGA CTTCCCTCAG CACGTGTCAT CTGACAGAGA AGTGCGGGCT 
GCAAGCACAG AAGCAGACAA AAGGCTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGAA 
GATGTGTTTC AGAGAATTGT TCATTTACAA GAAACATGCG ATTTGGAGAA GATAAAGCCT 
GAAGCCAGGC GATACTTGGA AAAGTCAATT AAAATGGGAA AAAGGAATGG ACTCCATCTT 
TCAGAACACA TAAGAAATGA AATCAAATCA ATGAAGAAGA GAATGAGTGA GCTATGCATT 
GACTTCAACA AGAACCTCAA TGAGGACGAC ACTTCCCTTG TATTCTCCAA GGCTGAACTT 
GGTGCTCTTC CTGATGACTT CATTGACAGT TTAGAAAAAA CAGATGAAGA CAAGTACAAA 
GTTACCTTAA AGTATCCTCA CTACTTCCCT GTCATGAAGA AATGCTGTGT CCCTGAAACT 
CGAAGGAAGA TGGAAATGGC TTTTCATACC AGGTGCAAAC AGGAAAACAC CGCAATTCTG 
CAACAGCTCC TCCCGCTGCG AGCTCAAGTG GCCAAGCTGC TGGGCTATAA CACCCATGCT 
GACTTTGTCC TTGAACTGAA CACTGCGAAG AGCACCAGCC GCGTGGCCGC CTTTCTAGAT 
GATTTAAGCC AGAAATTGAA ACCCTTGGGT GAGGCAGAAC GGGAGTTTAT TTTGAGCCTG 
AAGAAGAAGG AATGTGAAGA GCGAGGTTTT GAATACGACG GGAAGATCAA TGCATGGGAC 
CTCCATTACT ACATGACACA GACAGAGGAG CTCAAGTACT CGGTGGACCA GGAGTCCCTT 
AAGGAGTACT TCCCCATTGA GGTGGTCACG GAAGGCTTGC TAAGCATCTA CCAGGAGCTG 
CTGGGACTTT CATTTGAACA AGTGCCCGAT GCTCATGTTT GGAATAAGAG CGTTTCACTT 
TACACTGTGA AGGATAAAGC TACAGGAGAG GTCCTGGGGC AGTTCTACCT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA CCATGCAGCC TGCTTCGGTC TCCAGCCAGG CTGCCTTCTC 
CCTGATGGCA GTCGGATGAT GTCTGTGGCC GCCCTGGTGG TCAACTTCTC TCAGCCTGTA 
GCAGGTCGGC CCTCTCTCCT GAGACATGAT GAAGTGCGGA CTTACTTCCA CGAGTTCGGT 
CACGTCATGC ATCAGATCTG TGCGCAGACT GACTTTGCAC GATTCAGTGG AACAAACGTG 
GAAACTGACT TTGTAGAGGT GCCATCACAA ATGCTTGAAA ACTGGGTGTG GGACGTTGAT 
TCCCTGCGAA AACTGTCAAA GCATTATAAA GATGGACACC CTATCACAGA CGAGCTGCTC 
GAGAAGCTTG TTGCTTCTAG GCTGGTCAAC ACAGGTCTTC TGACGCTGCG CCAAATTGTT 
TTGAGCAAAG TTGACCAATC TCTCCATACC AATGCGACTC TGGATGCCGC GAGCGAATAC 
GCTAAATACT GCACAGAAAT TCTGGGAGTT GCAGCTACGC CAGGCACAAA TATGCCAGCT 
ACTTTTGGAC ACTTGGCAGG AGGGTATGAT GGCCAATATT ATGGATATCT TTGGAGTGAA 
GTGTTTTCCA TGGACATGTT TCACAGCTGT TTTAAAAAAG AGGGGATCAT GAATCCAGAG 
GTTGGAATGA AATACAGAAA CTTAATCCTG AAGCCTGGGG GGTCCCTGGA CGGCATGGAC 
ATGCTCCAGA ATTTCTTGCA ACGTGAGCCA AACCAAAAAG CATTCCTAAT GAGTCGAGGC 
CTGAATGGTT CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nln ( XM_006231880.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231942.1 Rattus norvegicus neurolysin (Nln), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24362
Clone ID Related Accession (Same CDS sequence)	XM_006231880.3
Accession Version	XM_006231880.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurolysin, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACCC TGTGCCTTTC GACTCTGCGA GGCCTCCACA GAGCTGGTGG CTCCAGGCTT 
CAGCTGACAA TGACACTGGG AAAGGAGCTG GCTTCTCCTC TTCAAGCTAT GTCTTCCTAC 
ACTGCGGCTG GCAGGAATGT TCTCAGATGG GACCTTTCTC CAGAGCAGAT CAAGACGAGG 
ACAGAGCAGC TCATAGCACA GACCAAGCAG GTGTACGACA CCGTGGGGAC AATCGCCCTG 
AAAGAAGTAA CTTATGAGAA CTGTCTGCAG GTGCTGGCTG ACATAGAAGT GACGTACATA 
GTGGAAAGGA CCATGCTGGA CTTCCCTCAG CACGTGTCAT CTGACAGAGA AGTGCGGGCT 
GCAAGCACAG AAGCAGACAA AAGGCTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGAA 
GATGTGTTTC AGAGAATTGT TCATTTACAA GAAACATGCG ATTTGGAGAA GATAAAGCCT 
GAAGCCAGGC GATACTTGGA AAAGTCAATT AAAATGGGAA AAAGGAATGG ACTCCATCTT 
TCAGAACACA TAAGAAATGA AATCAAATCA ATGAAGAAGA GAATGAGTGA GCTATGCATT 
GACTTCAACA AGAACCTCAA TGAGGACGAC ACTTCCCTTG TATTCTCCAA GGCTGAACTT 
GGTGCTCTTC CTGATGACTT CATTGACAGT TTAGAAAAAA CAGATGAAGA CAAGTACAAA 
GTTACCTTAA AGTATCCTCA CTACTTCCCT GTCATGAAGA AATGCTGTGT CCCTGAAACT 
CGAAGGAAGA TGGAAATGGC TTTTCATACC AGGTGCAAAC AGGAAAACAC CGCAATTCTG 
CAACAGCTCC TCCCGCTGCG AGCTCAAGTG GCCAAGCTGC TGGGCTATAA CACCCATGCT 
GACTTTGTCC TTGAACTGAA CACTGCGAAG AGCACCAGCC GCGTGGCCGC CTTTCTAGAT 
GATTTAAGCC AGAAATTGAA ACCCTTGGGT GAGGCAGAAC GGGAGTTTAT TTTGAGCCTG 
AAGAAGAAGG AATGTGAAGA GCGAGGTTTT GAATACGACG GGAAGATCAA TGCATGGGAC 
CTCCATTACT ACATGACACA GACAGAGGAG CTCAAGTACT CGGTGGACCA GGAGTCCCTT 
AAGGAGTACT TCCCCATTGA GGTGGTCACG GAAGGCTTGC TAAGCATCTA CCAGGAGCTG 
CTGGGACTTT CATTTGAACA AGTGCCCGAT GCTCATGTTT GGAATAAGAG CGTTTCACTT 
TACACTGTGA AGGATAAAGC TACAGGAGAG GTCCTGGGGC AGTTCTACCT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA CCATGCAGCC TGCTTCGGTC TCCAGCCAGG CTGCCTTCTC 
CCTGATGGCA GTCGGATGAT GTCTGTGGCC GCCCTGGTGG TCAACTTCTC TCAGCCTGTA 
GCAGGTCGGC CCTCTCTCCT GAGACATGAT GAAGTGCGGA CTTACTTCCA CGAGTTCGGT 
CACGTCATGC ATCAGATCTG TGCGCAGACT GACTTTGCAC GATTCAGTGG AACAAACGTG 
GAAACTGACT TTGTAGAGGT GCCATCACAA ATGCTTGAAA ACTGGGTGTG GGACGTTGAT 
TCCCTGCGAA AACTGTCAAA GCATTATAAA GATGGACACC CTATCACAGA CGAGCTGCTC 
GAGAAGCTTG TTGCTTCTAG GCTGGTCAAC ACAGGTCTTC TGACGCTGCG CCAAATTGTT 
TTGAGCAAAG TTGACCAATC TCTCCATACC AATGCGACTC TGGATGCCGC GAGCGAATAC 
GCTAAATACT GCACAGAAAT TCTGGGAGTT GCAGCTACGC CAGGCACAAA TATGCCAGCT 
ACTTTTGGAC ACTTGGCAGG AGGGTATGAT GGCCAATATT ATGGATATCT TTGGAGTGAA 
GTGTTTTCCA TGGACATGTT TCACAGCTGT TTTAAAAAAG AGGGGATCAT GAATCCAGAG 
GTTTCTTTGT ACGTTTTCTG TGGGAGGCAT CCTTTTTCTG TGGCCAGCGT ACTCCACCTA 
CTACAGAGTT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231942.1
CDS	189..2243
Translation	MITLCLSTLRGLHRAGGSRLQLTMTLGKELASPLQAMSSYTAAGRNVLRWDLSPEQIKTRTEQLIAQTKQVYDTVGTIALKEVTYENCLQVLADIEVTYIVERTMLDFPQHVSSDREVRAASTEADKRLSRFDIEMSMREDVFQRIVHLQETCDLEKIKPEARRYLEKSIKMGKRNGLHLSEHIRNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKAELGALPDDFIDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKQENTAILQQLLPLRAQVAKLLGYNTHADFVLELNTAKSTSRVAAFLDDLSQKLKPLGEAEREFILSLKKKECEERGFEYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQVPDAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMSVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRKLSKHYKDGHPITDELLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNATLDAASEYAKYCTEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFHSCFKKEGIMNPEVSLYVFCGRHPFSVASVLHLLQSL

Target ORF information:

RefSeq Version	XM_006231880.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurolysin (Nln), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231880.3

ORF Insert Sequence:

ATGATCACCC TGTGCCTTTC GACTCTGCGA GGCCTCCACA GAGCTGGTGG CTCCAGGCTT 
CAGCTGACAA TGACACTGGG AAAGGAGCTG GCTTCTCCTC TTCAAGCTAT GTCTTCCTAC 
ACTGCGGCTG GCAGGAATGT TCTCAGATGG GACCTTTCTC CAGAGCAGAT CAAGACGAGG 
ACAGAGCAGC TCATAGCACA GACCAAGCAG GTGTACGACA CCGTGGGGAC AATCGCCCTG 
AAAGAAGTAA CTTATGAGAA CTGTCTGCAG GTGCTGGCTG ACATAGAAGT GACGTACATA 
GTGGAAAGGA CCATGCTGGA CTTCCCTCAG CACGTGTCAT CTGACAGAGA AGTGCGGGCT 
GCAAGCACAG AAGCAGACAA AAGGCTTTCT CGTTTTGATA TTGAGATGAG CATGAGAGAA 
GATGTGTTTC AGAGAATTGT TCATTTACAA GAAACATGCG ATTTGGAGAA GATAAAGCCT 
GAAGCCAGGC GATACTTGGA AAAGTCAATT AAAATGGGAA AAAGGAATGG ACTCCATCTT 
TCAGAACACA TAAGAAATGA AATCAAATCA ATGAAGAAGA GAATGAGTGA GCTATGCATT 
GACTTCAACA AGAACCTCAA TGAGGACGAC ACTTCCCTTG TATTCTCCAA GGCTGAACTT 
GGTGCTCTTC CTGATGACTT CATTGACAGT TTAGAAAAAA CAGATGAAGA CAAGTACAAA 
GTTACCTTAA AGTATCCTCA CTACTTCCCT GTCATGAAGA AATGCTGTGT CCCTGAAACT 
CGAAGGAAGA TGGAAATGGC TTTTCATACC AGGTGCAAAC AGGAAAACAC CGCAATTCTG 
CAACAGCTCC TCCCGCTGCG AGCTCAAGTG GCCAAGCTGC TGGGCTATAA CACCCATGCT 
GACTTTGTCC TTGAACTGAA CACTGCGAAG AGCACCAGCC GCGTGGCCGC CTTTCTAGAT 
GATTTAAGCC AGAAATTGAA ACCCTTGGGT GAGGCAGAAC GGGAGTTTAT TTTGAGCCTG 
AAGAAGAAGG AATGTGAAGA GCGAGGTTTT GAATACGACG GGAAGATCAA TGCATGGGAC 
CTCCATTACT ACATGACACA GACAGAGGAG CTCAAGTACT CGGTGGACCA GGAGTCCCTT 
AAGGAGTACT TCCCCATTGA GGTGGTCACG GAAGGCTTGC TAAGCATCTA CCAGGAGCTG 
CTGGGACTTT CATTTGAACA AGTGCCCGAT GCTCATGTTT GGAATAAGAG CGTTTCACTT 
TACACTGTGA AGGATAAAGC TACAGGAGAG GTCCTGGGGC AGTTCTACCT GGACCTCTAT 
CCAAGGGAAG GAAAATACAA CCATGCAGCC TGCTTCGGTC TCCAGCCAGG CTGCCTTCTC 
CCTGATGGCA GTCGGATGAT GTCTGTGGCC GCCCTGGTGG TCAACTTCTC TCAGCCTGTA 
GCAGGTCGGC CCTCTCTCCT GAGACATGAT GAAGTGCGGA CTTACTTCCA CGAGTTCGGT 
CACGTCATGC ATCAGATCTG TGCGCAGACT GACTTTGCAC GATTCAGTGG AACAAACGTG 
GAAACTGACT TTGTAGAGGT GCCATCACAA ATGCTTGAAA ACTGGGTGTG GGACGTTGAT 
TCCCTGCGAA AACTGTCAAA GCATTATAAA GATGGACACC CTATCACAGA CGAGCTGCTC 
GAGAAGCTTG TTGCTTCTAG GCTGGTCAAC ACAGGTCTTC TGACGCTGCG CCAAATTGTT 
TTGAGCAAAG TTGACCAATC TCTCCATACC AATGCGACTC TGGATGCCGC GAGCGAATAC 
GCTAAATACT GCACAGAAAT TCTGGGAGTT GCAGCTACGC CAGGCACAAA TATGCCAGCT 
ACTTTTGGAC ACTTGGCAGG AGGGTATGAT GGCCAATATT ATGGATATCT TTGGAGTGAA 
GTGTTTTCCA TGGACATGTT TCACAGCTGT TTTAAAAAAG AGGGGATCAT GAATCCAGAG 
GTTTCTTTGT ACGTTTTCTG TGGGAGGCAT CCTTTTTCTG TGGCCAGCGT ACTCCACCTA 
CTACAGAGTT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nln ( XM_017590591.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446080.1 Rattus norvegicus neurolysin (Nln), transcript variant X3, mRNA.

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CloneID	ORa24363
Clone ID Related Accession (Same CDS sequence)	XM_017590591.1 , XM_006231881.2
Accession Version	XM_017590591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurolysin, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTGG GAAAGGAGCT GGCTTCTCCT CTTCAAGCTA TGTCTTCCTA CACTGCGGCT 
GGCAGGAATG TTCTCAGATG GGACCTTTCT CCAGAGCAGA TCAAGACGAG GACAGAGCAG 
CTCATAGCAC AGACCAAGCA GGTGTACGAC ACCGTGGGGA CAATCGCCCT GAAAGAAGTA 
ACTTATGAGA ACTGTCTGCA GGTGCTGGCT GACATAGAAG TGACGTACAT AGTGGAAAGG 
ACCATGCTGG ACTTCCCTCA GCACGTGTCA TCTGACAGAG AAGTGCGGGC TGCAAGCACA 
GAAGCAGACA AAAGGCTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGA AGATGTGTTT 
CAGAGAATTG TTCATTTACA AGAAACATGC GATTTGGAGA AGATAAAGCC TGAAGCCAGG 
CGATACTTGG AAAAGTCAAT TAAAATGGGA AAAAGGAATG GACTCCATCT TTCAGAACAC 
ATAAGAAATG AAATCAAATC AATGAAGAAG AGAATGAGTG AGCTATGCAT TGACTTCAAC 
AAGAACCTCA ATGAGGACGA CACTTCCCTT GTATTCTCCA AGGCTGAACT TGGTGCTCTT 
CCTGATGACT TCATTGACAG TTTAGAAAAA ACAGATGAAG ACAAGTACAA AGTTACCTTA 
AAGTATCCTC ACTACTTCCC TGTCATGAAG AAATGCTGTG TCCCTGAAAC TCGAAGGAAG 
ATGGAAATGG CTTTTCATAC CAGGTGCAAA CAGGAAAACA CCGCAATTCT GCAACAGCTC 
CTCCCGCTGC GAGCTCAAGT GGCCAAGCTG CTGGGCTATA ACACCCATGC TGACTTTGTC 
CTTGAACTGA ACACTGCGAA GAGCACCAGC CGCGTGGCCG CCTTTCTAGA TGATTTAAGC 
CAGAAATTGA AACCCTTGGG TGAGGCAGAA CGGGAGTTTA TTTTGAGCCT GAAGAAGAAG 
GAATGTGAAG AGCGAGGTTT TGAATACGAC GGGAAGATCA ATGCATGGGA CCTCCATTAC 
TACATGACAC AGACAGAGGA GCTCAAGTAC TCGGTGGACC AGGAGTCCCT TAAGGAGTAC 
TTCCCCATTG AGGTGGTCAC GGAAGGCTTG CTAAGCATCT ACCAGGAGCT GCTGGGACTT 
TCATTTGAAC AAGTGCCCGA TGCTCATGTT TGGAATAAGA GCGTTTCACT TTACACTGTG 
AAGGATAAAG CTACAGGAGA GGTCCTGGGG CAGTTCTACC TGGACCTCTA TCCAAGGGAA 
GGAAAATACA ACCATGCAGC CTGCTTCGGT CTCCAGCCAG GCTGCCTTCT CCCTGATGGC 
AGTCGGATGA TGTCTGTGGC CGCCCTGGTG GTCAACTTCT CTCAGCCTGT AGCAGGTCGG 
CCCTCTCTCC TGAGACATGA TGAAGTGCGG ACTTACTTCC ACGAGTTCGG TCACGTCATG 
CATCAGATCT GTGCGCAGAC TGACTTTGCA CGATTCAGTG GAACAAACGT GGAAACTGAC 
TTTGTAGAGG TGCCATCACA AATGCTTGAA AACTGGGTGT GGGACGTTGA TTCCCTGCGA 
AAACTGTCAA AGCATTATAA AGATGGACAC CCTATCACAG ACGAGCTGCT CGAGAAGCTT 
GTTGCTTCTA GGCTGGTCAA CACAGGTCTT CTGACGCTGC GCCAAATTGT TTTGAGCAAA 
GTTGACCAAT CTCTCCATAC CAATGCGACT CTGGATGCCG CGAGCGAATA CGCTAAATAC 
TGCACAGAAA TTCTGGGAGT TGCAGCTACG CCAGGCACAA ATATGCCAGC TACTTTTGGA 
CACTTGGCAG GAGGGTATGA TGGCCAATAT TATGGATATC TTTGGAGTGA AGTGTTTTCC 
ATGGACATGT TTCACAGCTG TTTTAAAAAA GAGGGGATCA TGAATCCAGA GGTTGGAATG 
AAATACAGAA ACTTAATCCT GAAGCCTGGG GGGTCCCTGG ACGGCATGGA CATGCTCCAG 
AATTTCTTGC AACGTGAGCC AAACCAAAAA GCATTCCTAA TGAGTCGAGG CCTGAATGGT 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446080.1
CDS	125..2170
Translation	MTLGKELASPLQAMSSYTAAGRNVLRWDLSPEQIKTRTEQLIAQTKQVYDTVGTIALKEVTYENCLQVLADIEVTYIVERTMLDFPQHVSSDREVRAASTEADKRLSRFDIEMSMREDVFQRIVHLQETCDLEKIKPEARRYLEKSIKMGKRNGLHLSEHIRNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKAELGALPDDFIDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKQENTAILQQLLPLRAQVAKLLGYNTHADFVLELNTAKSTSRVAAFLDDLSQKLKPLGEAEREFILSLKKKECEERGFEYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQVPDAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMSVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRKLSKHYKDGHPITDELLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNATLDAASEYAKYCTEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFHSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLQREPNQKAFLMSRGLNGS

Target ORF information:

RefSeq Version	XM_017590591.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurolysin (Nln), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590591.1

ORF Insert Sequence:

ATGACACTGG GAAAGGAGCT GGCTTCTCCT CTTCAAGCTA TGTCTTCCTA CACTGCGGCT 
GGCAGGAATG TTCTCAGATG GGACCTTTCT CCAGAGCAGA TCAAGACGAG GACAGAGCAG 
CTCATAGCAC AGACCAAGCA GGTGTACGAC ACCGTGGGGA CAATCGCCCT GAAAGAAGTA 
ACTTATGAGA ACTGTCTGCA GGTGCTGGCT GACATAGAAG TGACGTACAT AGTGGAAAGG 
ACCATGCTGG ACTTCCCTCA GCACGTGTCA TCTGACAGAG AAGTGCGGGC TGCAAGCACA 
GAAGCAGACA AAAGGCTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGA AGATGTGTTT 
CAGAGAATTG TTCATTTACA AGAAACATGC GATTTGGAGA AGATAAAGCC TGAAGCCAGG 
CGATACTTGG AAAAGTCAAT TAAAATGGGA AAAAGGAATG GACTCCATCT TTCAGAACAC 
ATAAGAAATG AAATCAAATC AATGAAGAAG AGAATGAGTG AGCTATGCAT TGACTTCAAC 
AAGAACCTCA ATGAGGACGA CACTTCCCTT GTATTCTCCA AGGCTGAACT TGGTGCTCTT 
CCTGATGACT TCATTGACAG TTTAGAAAAA ACAGATGAAG ACAAGTACAA AGTTACCTTA 
AAGTATCCTC ACTACTTCCC TGTCATGAAG AAATGCTGTG TCCCTGAAAC TCGAAGGAAG 
ATGGAAATGG CTTTTCATAC CAGGTGCAAA CAGGAAAACA CCGCAATTCT GCAACAGCTC 
CTCCCGCTGC GAGCTCAAGT GGCCAAGCTG CTGGGCTATA ACACCCATGC TGACTTTGTC 
CTTGAACTGA ACACTGCGAA GAGCACCAGC CGCGTGGCCG CCTTTCTAGA TGATTTAAGC 
CAGAAATTGA AACCCTTGGG TGAGGCAGAA CGGGAGTTTA TTTTGAGCCT GAAGAAGAAG 
GAATGTGAAG AGCGAGGTTT TGAATACGAC GGGAAGATCA ATGCATGGGA CCTCCATTAC 
TACATGACAC AGACAGAGGA GCTCAAGTAC TCGGTGGACC AGGAGTCCCT TAAGGAGTAC 
TTCCCCATTG AGGTGGTCAC GGAAGGCTTG CTAAGCATCT ACCAGGAGCT GCTGGGACTT 
TCATTTGAAC AAGTGCCCGA TGCTCATGTT TGGAATAAGA GCGTTTCACT TTACACTGTG 
AAGGATAAAG CTACAGGAGA GGTCCTGGGG CAGTTCTACC TGGACCTCTA TCCAAGGGAA 
GGAAAATACA ACCATGCAGC CTGCTTCGGT CTCCAGCCAG GCTGCCTTCT CCCTGATGGC 
AGTCGGATGA TGTCTGTGGC CGCCCTGGTG GTCAACTTCT CTCAGCCTGT AGCAGGTCGG 
CCCTCTCTCC TGAGACATGA TGAAGTGCGG ACTTACTTCC ACGAGTTCGG TCACGTCATG 
CATCAGATCT GTGCGCAGAC TGACTTTGCA CGATTCAGTG GAACAAACGT GGAAACTGAC 
TTTGTAGAGG TGCCATCACA AATGCTTGAA AACTGGGTGT GGGACGTTGA TTCCCTGCGA 
AAACTGTCAA AGCATTATAA AGATGGACAC CCTATCACAG ACGAGCTGCT CGAGAAGCTT 
GTTGCTTCTA GGCTGGTCAA CACAGGTCTT CTGACGCTGC GCCAAATTGT TTTGAGCAAA 
GTTGACCAAT CTCTCCATAC CAATGCGACT CTGGATGCCG CGAGCGAATA CGCTAAATAC 
TGCACAGAAA TTCTGGGAGT TGCAGCTACG CCAGGCACAA ATATGCCAGC TACTTTTGGA 
CACTTGGCAG GAGGGTATGA TGGCCAATAT TATGGATATC TTTGGAGTGA AGTGTTTTCC 
ATGGACATGT TTCACAGCTG TTTTAAAAAA GAGGGGATCA TGAATCCAGA GGTTGGAATG 
AAATACAGAA ACTTAATCCT GAAGCCTGGG GGGTCCCTGG ACGGCATGGA CATGCTCCAG 
AATTTCTTGC AACGTGAGCC AAACCAAAAA GCATTCCTAA TGAGTCGAGG CCTGAATGGT 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nln ( XM_006231881.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231943.1 Rattus norvegicus neurolysin (Nln), transcript variant X2, mRNA.

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CloneID	ORa24363
Clone ID Related Accession (Same CDS sequence)	XM_017590591.1 , XM_006231881.2
Accession Version	XM_006231881.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurolysin, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006231881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTGG GAAAGGAGCT GGCTTCTCCT CTTCAAGCTA TGTCTTCCTA CACTGCGGCT 
GGCAGGAATG TTCTCAGATG GGACCTTTCT CCAGAGCAGA TCAAGACGAG GACAGAGCAG 
CTCATAGCAC AGACCAAGCA GGTGTACGAC ACCGTGGGGA CAATCGCCCT GAAAGAAGTA 
ACTTATGAGA ACTGTCTGCA GGTGCTGGCT GACATAGAAG TGACGTACAT AGTGGAAAGG 
ACCATGCTGG ACTTCCCTCA GCACGTGTCA TCTGACAGAG AAGTGCGGGC TGCAAGCACA 
GAAGCAGACA AAAGGCTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGA AGATGTGTTT 
CAGAGAATTG TTCATTTACA AGAAACATGC GATTTGGAGA AGATAAAGCC TGAAGCCAGG 
CGATACTTGG AAAAGTCAAT TAAAATGGGA AAAAGGAATG GACTCCATCT TTCAGAACAC 
ATAAGAAATG AAATCAAATC AATGAAGAAG AGAATGAGTG AGCTATGCAT TGACTTCAAC 
AAGAACCTCA ATGAGGACGA CACTTCCCTT GTATTCTCCA AGGCTGAACT TGGTGCTCTT 
CCTGATGACT TCATTGACAG TTTAGAAAAA ACAGATGAAG ACAAGTACAA AGTTACCTTA 
AAGTATCCTC ACTACTTCCC TGTCATGAAG AAATGCTGTG TCCCTGAAAC TCGAAGGAAG 
ATGGAAATGG CTTTTCATAC CAGGTGCAAA CAGGAAAACA CCGCAATTCT GCAACAGCTC 
CTCCCGCTGC GAGCTCAAGT GGCCAAGCTG CTGGGCTATA ACACCCATGC TGACTTTGTC 
CTTGAACTGA ACACTGCGAA GAGCACCAGC CGCGTGGCCG CCTTTCTAGA TGATTTAAGC 
CAGAAATTGA AACCCTTGGG TGAGGCAGAA CGGGAGTTTA TTTTGAGCCT GAAGAAGAAG 
GAATGTGAAG AGCGAGGTTT TGAATACGAC GGGAAGATCA ATGCATGGGA CCTCCATTAC 
TACATGACAC AGACAGAGGA GCTCAAGTAC TCGGTGGACC AGGAGTCCCT TAAGGAGTAC 
TTCCCCATTG AGGTGGTCAC GGAAGGCTTG CTAAGCATCT ACCAGGAGCT GCTGGGACTT 
TCATTTGAAC AAGTGCCCGA TGCTCATGTT TGGAATAAGA GCGTTTCACT TTACACTGTG 
AAGGATAAAG CTACAGGAGA GGTCCTGGGG CAGTTCTACC TGGACCTCTA TCCAAGGGAA 
GGAAAATACA ACCATGCAGC CTGCTTCGGT CTCCAGCCAG GCTGCCTTCT CCCTGATGGC 
AGTCGGATGA TGTCTGTGGC CGCCCTGGTG GTCAACTTCT CTCAGCCTGT AGCAGGTCGG 
CCCTCTCTCC TGAGACATGA TGAAGTGCGG ACTTACTTCC ACGAGTTCGG TCACGTCATG 
CATCAGATCT GTGCGCAGAC TGACTTTGCA CGATTCAGTG GAACAAACGT GGAAACTGAC 
TTTGTAGAGG TGCCATCACA AATGCTTGAA AACTGGGTGT GGGACGTTGA TTCCCTGCGA 
AAACTGTCAA AGCATTATAA AGATGGACAC CCTATCACAG ACGAGCTGCT CGAGAAGCTT 
GTTGCTTCTA GGCTGGTCAA CACAGGTCTT CTGACGCTGC GCCAAATTGT TTTGAGCAAA 
GTTGACCAAT CTCTCCATAC CAATGCGACT CTGGATGCCG CGAGCGAATA CGCTAAATAC 
TGCACAGAAA TTCTGGGAGT TGCAGCTACG CCAGGCACAA ATATGCCAGC TACTTTTGGA 
CACTTGGCAG GAGGGTATGA TGGCCAATAT TATGGATATC TTTGGAGTGA AGTGTTTTCC 
ATGGACATGT TTCACAGCTG TTTTAAAAAA GAGGGGATCA TGAATCCAGA GGTTGGAATG 
AAATACAGAA ACTTAATCCT GAAGCCTGGG GGGTCCCTGG ACGGCATGGA CATGCTCCAG 
AATTTCTTGC AACGTGAGCC AAACCAAAAA GCATTCCTAA TGAGTCGAGG CCTGAATGGT 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231943.1
CDS	337..2382
Translation	MTLGKELASPLQAMSSYTAAGRNVLRWDLSPEQIKTRTEQLIAQTKQVYDTVGTIALKEVTYENCLQVLADIEVTYIVERTMLDFPQHVSSDREVRAASTEADKRLSRFDIEMSMREDVFQRIVHLQETCDLEKIKPEARRYLEKSIKMGKRNGLHLSEHIRNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKAELGALPDDFIDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKQENTAILQQLLPLRAQVAKLLGYNTHADFVLELNTAKSTSRVAAFLDDLSQKLKPLGEAEREFILSLKKKECEERGFEYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQVPDAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMSVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRKLSKHYKDGHPITDELLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNATLDAASEYAKYCTEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFHSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLQREPNQKAFLMSRGLNGS

Target ORF information:

RefSeq Version	XM_006231881.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurolysin (Nln), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231881.2

ORF Insert Sequence:

ATGACACTGG GAAAGGAGCT GGCTTCTCCT CTTCAAGCTA TGTCTTCCTA CACTGCGGCT 
GGCAGGAATG TTCTCAGATG GGACCTTTCT CCAGAGCAGA TCAAGACGAG GACAGAGCAG 
CTCATAGCAC AGACCAAGCA GGTGTACGAC ACCGTGGGGA CAATCGCCCT GAAAGAAGTA 
ACTTATGAGA ACTGTCTGCA GGTGCTGGCT GACATAGAAG TGACGTACAT AGTGGAAAGG 
ACCATGCTGG ACTTCCCTCA GCACGTGTCA TCTGACAGAG AAGTGCGGGC TGCAAGCACA 
GAAGCAGACA AAAGGCTTTC TCGTTTTGAT ATTGAGATGA GCATGAGAGA AGATGTGTTT 
CAGAGAATTG TTCATTTACA AGAAACATGC GATTTGGAGA AGATAAAGCC TGAAGCCAGG 
CGATACTTGG AAAAGTCAAT TAAAATGGGA AAAAGGAATG GACTCCATCT TTCAGAACAC 
ATAAGAAATG AAATCAAATC AATGAAGAAG AGAATGAGTG AGCTATGCAT TGACTTCAAC 
AAGAACCTCA ATGAGGACGA CACTTCCCTT GTATTCTCCA AGGCTGAACT TGGTGCTCTT 
CCTGATGACT TCATTGACAG TTTAGAAAAA ACAGATGAAG ACAAGTACAA AGTTACCTTA 
AAGTATCCTC ACTACTTCCC TGTCATGAAG AAATGCTGTG TCCCTGAAAC TCGAAGGAAG 
ATGGAAATGG CTTTTCATAC CAGGTGCAAA CAGGAAAACA CCGCAATTCT GCAACAGCTC 
CTCCCGCTGC GAGCTCAAGT GGCCAAGCTG CTGGGCTATA ACACCCATGC TGACTTTGTC 
CTTGAACTGA ACACTGCGAA GAGCACCAGC CGCGTGGCCG CCTTTCTAGA TGATTTAAGC 
CAGAAATTGA AACCCTTGGG TGAGGCAGAA CGGGAGTTTA TTTTGAGCCT GAAGAAGAAG 
GAATGTGAAG AGCGAGGTTT TGAATACGAC GGGAAGATCA ATGCATGGGA CCTCCATTAC 
TACATGACAC AGACAGAGGA GCTCAAGTAC TCGGTGGACC AGGAGTCCCT TAAGGAGTAC 
TTCCCCATTG AGGTGGTCAC GGAAGGCTTG CTAAGCATCT ACCAGGAGCT GCTGGGACTT 
TCATTTGAAC AAGTGCCCGA TGCTCATGTT TGGAATAAGA GCGTTTCACT TTACACTGTG 
AAGGATAAAG CTACAGGAGA GGTCCTGGGG CAGTTCTACC TGGACCTCTA TCCAAGGGAA 
GGAAAATACA ACCATGCAGC CTGCTTCGGT CTCCAGCCAG GCTGCCTTCT CCCTGATGGC 
AGTCGGATGA TGTCTGTGGC CGCCCTGGTG GTCAACTTCT CTCAGCCTGT AGCAGGTCGG 
CCCTCTCTCC TGAGACATGA TGAAGTGCGG ACTTACTTCC ACGAGTTCGG TCACGTCATG 
CATCAGATCT GTGCGCAGAC TGACTTTGCA CGATTCAGTG GAACAAACGT GGAAACTGAC 
TTTGTAGAGG TGCCATCACA AATGCTTGAA AACTGGGTGT GGGACGTTGA TTCCCTGCGA 
AAACTGTCAA AGCATTATAA AGATGGACAC CCTATCACAG ACGAGCTGCT CGAGAAGCTT 
GTTGCTTCTA GGCTGGTCAA CACAGGTCTT CTGACGCTGC GCCAAATTGT TTTGAGCAAA 
GTTGACCAAT CTCTCCATAC CAATGCGACT CTGGATGCCG CGAGCGAATA CGCTAAATAC 
TGCACAGAAA TTCTGGGAGT TGCAGCTACG CCAGGCACAA ATATGCCAGC TACTTTTGGA 
CACTTGGCAG GAGGGTATGA TGGCCAATAT TATGGATATC TTTGGAGTGA AGTGTTTTCC 
ATGGACATGT TTCACAGCTG TTTTAAAAAA GAGGGGATCA TGAATCCAGA GGTTGGAATG 
AAATACAGAA ACTTAATCCT GAAGCCTGGG GGGTCCCTGG ACGGCATGGA CATGCTCCAG 
AATTTCTTGC AACGTGAGCC AAACCAAAAA GCATTCCTAA TGAGTCGAGG CCTGAATGGT 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Allosteric inhibition of the neuropeptidase neurolysin.
The Journal of biological chemistry289(51)35605-19(2014 Dec)
Hines CS,Ray K,Schmidt JJ,Xiong F,Feenstra RW,Pras-Raves M,de Moes JP,Lange JH,Melikishvili M,Fried MG,Mortenson P,Charlton M,Patel Y,Courtney SM,Kruse CG,Rodgers DW

Hepatic scavenger receptor BI protects against polymicrobial-induced sepsis through promoting LPS clearance in mice.
The Journal of biological chemistry289(21)14666-73(2014 May)
Guo L,Zheng Z,Ai J,Huang B,Li XA

Neurolysin knockout mice generation and initial phenotype characterization.
The Journal of biological chemistry289(22)15426-40(2014 May)
Cavalcanti DM,Castro LM,Rosa Neto JC,Seelaender M,Neves RX,Oliveira V,Forti FL,Iwai LK,Gozzo FC,Todiras M,Schadock I,Barros CC,Bader M,Ferro ES

Pharmacological characterization of a novel non-AT1, non-AT2 angiotensin binding site identified as neurolysin.
Endocrine44(2)525-31(2013 Oct)
Swindle JD,Santos KL,Speth RC

Identification of membrane-bound variant of metalloendopeptidase neurolysin (EC 3.4.24.16) as the non-angiotensin type 1 (non-AT1), non-AT2 angiotensin binding site.
The Journal of biological chemistry287(1)114-22(2012 Jan)
Wangler NJ,Santos KL,Schadock I,Hagen FK,Escher E,Bader M,Speth RC,Karamyan VT

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Nln cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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