Slc25a20 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc25a20
Entrez Gene ID	117035
Full Name	solute carrier family 25 member 20
General protein information	Preferred Names solute carrier family 25 member 20 Names mitochondrial carnitine/acylcarnitine carrier protein CAC carnitine/acylcarnitine translocase solute carrier family 25 (carnitine/acylcarnitine translocase), member 20 solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	carnitine carrier protein, component of the mitochondrial inner membrane; trasfers fatty acylcarnitines into mitochondria; critical for fatty acid oxidation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053965.2	NP_446417.2	mitochondrial carnitine/acylcarnitine carrier protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SLC25A20	XP_863142.1
Gallus gallus (chicken)	SLC25A20	XP_414400.1
Neurospora crassa	NCU07263	XP_962117.1
Xenopus tropicalis (tropical clawed frog)	slc25a20.2	NP_989099.1
Rattus norvegicus (Norway rat)	Slc25a20	NP_446417.2
Caenorhabditis elegans (roundworm)	dif-1	NP_501223.1
Homo sapiens (human)	SLC25A20	NP_000378.1
	SLC25A20	XP_001111751.1
Bos taurus (cattle)	SLC25A20	NP_001071404.1
Mus musculus (house mouse)	Slc25a20	NP_065266.1
Danio rerio (zebrafish)	slc25a20	NP_957153.1
Pan troglodytes (chimpanzee)	SLC25A20	XP_516446.3
Saccharomyces cerevisiae (baker's yeast)	CRC1	NP_014743.1
Kluyveromyces lactis	KLLA0C13431g	XP_452799.1
Eremothecium gossypii	AGOS_ADL264C	NP_983832.1
Arabidopsis thaliana (thale cress)	BOU	NP_568670.1
Drosophila melanogaster (fruit fly)	colt	NP_477221.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006104	XP_316164.4
Magnaporthe oryzae (rice blast fungus)	MGG_10492	XP_003713396.1
Oryza sativa (rice)	Os10g0573700	NP_001065471.1

Gene Ontology
Bibliography

Related articles in PubMed

Nitric oxide inhibits the mitochondrial carnitine/acylcarnitine carrier through reversible S-nitrosylation of cysteine 136.
Tonazzi A, Giangregorio N, Console L, De Palma A, Indiveri C
Biochimica et biophysica acta1858(7)475-482(2017 07)

Post-translational modification by acetylation regulates the mitochondrial carnitine/acylcarnitine transport protein.
Giangregorio N, Tonazzi A, Console L, Indiveri C
Molecular and cellular biochemistry426(1-2)65-73(2017 Feb)

Highly variable penetrance of abnormal phenotypes in embryonic lethal knockout mice.
Wilson R, Geyer SH, Reissig L, Rose J, Szumska D, Hardman E, Prin F, McGuire C, Ramirez-Solis R, White J, Galli A, Tudor C, Tuck E, Mazzeo CI, Smith JC, Robertson E, Adams DJ, Mohun T, Weninger WJ
Wellcome open research11(2016)

Carnitine/acylcarnitine translocase and carnitine palmitoyltransferase 2 form a complex in the inner mitochondrial membrane.
Console L, Giangregorio N, Indiveri C, Tonazzi A
Molecular and cellular biochemistry394(1-2)307-14(2014 Sep)

Identification by site-directed mutagenesis of a hydrophobic binding site of the mitochondrial carnitine/acylcarnitine carrier involved in the interaction with acyl groups.
Tonazzi A, Console L, Giangregorio N, Indiveri C, Palmieri F
Biochimica et biophysica acta1817(5)697-704(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inhibition by the NO-releasing compound GSNO suggests that C136 S-nitrosylation of native and recombinant CACT may have a role in the regulation of the beta-oxidation pathway.
Title: Nitric oxide inhibits the mitochondrial carnitine/acylcarnitine carrier through reversible S-nitrosylation of cysteine 136.

The experimental data indicate that acetylation of CACT inhibits its transport activity, and thus may contribute to the regulation of the mitochondrial beta-oxidation pathway.
Title: Post-translational modification by acetylation regulates the mitochondrial carnitine/acylcarnitine transport protein.

Report interaction between carnitine palmitoyltransferase 2 and carnitine/acylcarnitine translocase supporting the channeling of acylcarnitines and carnitine at level of the inner mitochondrial membrane.
Title: Carnitine/acylcarnitine translocase and carnitine palmitoyltransferase 2 form a complex in the inner mitochondrial membrane.

The data demonstrated that V25, P78, V82, M85 and C89 are involved in the acyl chain binding to the CAC in c-state.
Title: Identification by site-directed mutagenesis of a hydrophobic binding site of the mitochondrial carnitine/acylcarnitine carrier involved in the interaction with acyl groups.

Cys(58), Cys(136), and Cys(155) become close in the tertiary structure of the CAC during its catalytic cycle.
Title: Identification by site-directed mutagenesis and chemical modification of three vicinal cysteine residues in rat mitochondrial carnitine/acylcarnitine transporter.

The following Slc25a20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc25a20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12478	NM_053965.2 Latest version!	Rattus norvegicus solute carrier family 25 member 20 (Slc25a20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc25a20 ( NM_053965.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446417.2 Rattus norvegicus solute carrier family 25 member 20 (Slc25a20), mRNA.

Price & Availability↑

CloneID	ORa12478
Clone ID Related Accession (Same CDS sequence)	NM_053965.2
Accession Version	NM_053965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial carnitine/acylcarnitine carrier protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081749.1. On Sep 16, 2004 this sequence version replaced NM_053965.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081749.1, FQ209681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCAGAGG AGCCGAAACC CATCAGTCCG CTTAAGAACC TCCTTGCCGG CGGCTTTGGC 
GGCGTGTGCC TGGTGTTTGT GGGGCACCCC TTGGACACGG TCAAGGTCCG ACTGCAGACA 
CAACCACCAA GTTTGCCTGG GCAGCCACCT ATGTACTCTG GGACCATCGA CTGTTTCCGG 
AAGACTCTTT TTAGAGAGGG CATCACAGGG CTGTATCGGG GCATGGCTGC ACCCATCATT 
GGAGTCACCC CTATGTTTGC CGTGTGCTTC TTTGGGTTTG GTTTGGGGAA GAGACTACAG 
CAGAAATCTC CAGAGGATGA ACTTACCTAC CCGCAGCTGT TTACAGCTGG GATGTTATCT 
GGTGTGTTCA CCACAGGAAT CATGACCCCT GGAGAACGGA TCAAATGCTT ACTGCAGATT 
CAGGCTTCTT CAGGGAAAAA CAAATACAGT GGTACCTTGG ACTGTGCAAA GAAGCTGTAT 
CAGGAGTTTG GGATCCGTGG CTTCTACAAA GGGACTGTGC TCACTCTCAT GCGAGATGTT 
CCTGCCAGTG GGATGTATTT CATGACATAC GAATGGCTTA AAAATCTCTT CACTCCACAG 
GGAAAGAGTG TCCATGACCT CAGTGTGCCT CGGGTCCTGG TGGCTGGCGG CTTTGCAGGG 
ATCTTCAATT GGGTTGTGGC AATCCCCCCA GACGTGCTCA AGTCTCGATT CCAGACCGCA 
CCTCCTGGAA AATATCCTAA TGGTTTCAGA GATGTGTTGA GAGAGCTGAT CCGAGAAGAA 
GGAGTCACCT CCTTGTACAA AGGGTTCAAT GCAGTCATGA TCCGAGCCTT CCCAGCCAAT 
GCTGCCTGTT TCCTTGGCTT TGAAATTGCC ATGAAGATCC TCAATTGGAT TGCCCCCAAC 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446417.2
CDS	101..1006
Translation	MAEEPKPISPLKNLLAGGFGGVCLVFVGHPLDTVKVRLQTQPPSLPGQPPMYSGTIDCFRKTLFREGITGLYRGMAAPIIGVTPMFAVCFFGFGLGKRLQQKSPEDELTYPQLFTAGMLSGVFTTGIMTPGERIKCLLQIQASSGKNKYSGTLDCAKKLYQEFGIRGFYKGTVLTLMRDVPASGMYFMTYEWLKNLFTPQGKSVHDLSVPRVLVAGGFAGIFNWVVAIPPDVLKSRFQTAPPGKYPNGFRDVLRELIREEGVTSLYKGFNAVMIRAFPANAACFLGFEIAMKILNWIAPNL

Target ORF information:

RefSeq Version	NM_053965.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 25 member 20 (Slc25a20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053965.2

ORF Insert Sequence:

ATGGCAGAGG AGCCGAAACC CATCAGTCCG CTTAAGAACC TCCTTGCCGG CGGCTTTGGC 
GGCGTGTGCC TGGTGTTTGT GGGGCACCCC TTGGACACGG TCAAGGTCCG ACTGCAGACA 
CAACCACCAA GTTTGCCTGG GCAGCCACCT ATGTACTCTG GGACCATCGA CTGTTTCCGG 
AAGACTCTTT TTAGAGAGGG CATCACAGGG CTGTATCGGG GCATGGCTGC ACCCATCATT 
GGAGTCACCC CTATGTTTGC CGTGTGCTTC TTTGGGTTTG GTTTGGGGAA GAGACTACAG 
CAGAAATCTC CAGAGGATGA ACTTACCTAC CCGCAGCTGT TTACAGCTGG GATGTTATCT 
GGTGTGTTCA CCACAGGAAT CATGACCCCT GGAGAACGGA TCAAATGCTT ACTGCAGATT 
CAGGCTTCTT CAGGGAAAAA CAAATACAGT GGTACCTTGG ACTGTGCAAA GAAGCTGTAT 
CAGGAGTTTG GGATCCGTGG CTTCTACAAA GGGACTGTGC TCACTCTCAT GCGAGATGTT 
CCTGCCAGTG GGATGTATTT CATGACATAC GAATGGCTTA AAAATCTCTT CACTCCACAG 
GGAAAGAGTG TCCATGACCT CAGTGTGCCT CGGGTCCTGG TGGCTGGCGG CTTTGCAGGG 
ATCTTCAATT GGGTTGTGGC AATCCCCCCA GACGTGCTCA AGTCTCGATT CCAGACCGCA 
CCTCCTGGAA AATATCCTAA TGGTTTCAGA GATGTGTTGA GAGAGCTGAT CCGAGAAGAA 
GGAGTCACCT CCTTGTACAA AGGGTTCAAT GCAGTCATGA TCCGAGCCTT CCCAGCCAAT 
GCTGCCTGTT TCCTTGGCTT TGAAATTGCC ATGAAGATCC TCAATTGGAT TGCCCCCAAC 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nitric oxide inhibits the mitochondrial carnitine/acylcarnitine carrier through reversible S-nitrosylation of cysteine 136.
Biochimica et biophysica acta1858(7)475-482(2017 07)
Tonazzi A,Giangregorio N,Console L,De Palma A,Indiveri C

Post-translational modification by acetylation regulates the mitochondrial carnitine/acylcarnitine transport protein.
Molecular and cellular biochemistry426(1-2)65-73(2017 Feb)
Giangregorio N,Tonazzi A,Console L,Indiveri C

Highly variable penetrance of abnormal phenotypes in embryonic lethal knockout mice.
Wellcome open research11(2016)
Wilson R,Geyer SH,Reissig L,Rose J,Szumska D,Hardman E,Prin F,McGuire C,Ramirez-Solis R,White J,Galli A,Tudor C,Tuck E,Mazzeo CI,Smith JC,Robertson E,Adams DJ,Mohun T,Weninger WJ

Carnitine/acylcarnitine translocase and carnitine palmitoyltransferase 2 form a complex in the inner mitochondrial membrane.
Molecular and cellular biochemistry394(1-2)307-14(2014 Sep)
Console L,Giangregorio N,Indiveri C,Tonazzi A

Identification by site-directed mutagenesis of a hydrophobic binding site of the mitochondrial carnitine/acylcarnitine carrier involved in the interaction with acyl groups.
Biochimica et biophysica acta1817(5)697-704(2012 May)
Tonazzi A,Console L,Giangregorio N,Indiveri C,Palmieri F

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Slc25a20 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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