Ptf1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptf1a
Entrez Gene ID	117034
Full Name	pancreas specific transcription factor, 1a
General protein information	Preferred Names pancreas specific transcription factor, 1a Names pancreas transcription factor 1 subunit alpha PTF1-p48 bHLH transcription factor p48 p48 DNA-binding subunit of transcription factor PTF1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.3
Summary	DNA-binding subunit of the transcription factor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053964.1	NP_446416.1	pancreas transcription factor 1 subunit alpha

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ptf1a	NP_997524.1
Bos taurus (cattle)	PTF1A	XP_002707767.1
Mus musculus (house mouse)	Ptf1a	NP_061279.2
Xenopus tropicalis (tropical clawed frog)	ptf1a	XP_002933181.2
Homo sapiens (human)	PTF1A	NP_835455.1
Rattus norvegicus (Norway rat)	Ptf1a	NP_446416.1
Pan troglodytes (chimpanzee)	PTF1A	XP_001146416.2
Canis lupus familiaris (dog)	PTF1A	XP_850386.2

Gene Ontology
Bibliography

Related articles in PubMed

The nuclear hormone receptor family member NR5A2 controls aspects of multipotent progenitor cell formation and acinar differentiation during pancreatic organogenesis.
Hale MA, Swift GH, Hoang CQ, Deering TG, Masui T, Lee YK, Xue J, MacDonald RJ
Development (Cambridge, England)141(16)3123-33(2014 Aug)

Genome-wide expression analysis of Ptf1a- and Ascl1-deficient mice reveals new markers for distinct dorsal horn interneuron populations contributing to nociceptive reflex plasticity.
Wildner H, Das Gupta R, Br?hl D, Heppenstall PA, Zeilhofer HU, Birchmeier C
The Journal of neuroscience : the official journal of the Society for Neuroscience33(17)7299-307(2013 Apr)

Prdm13 mediates the balance of inhibitory and excitatory neurons in somatosensory circuits.
Chang JC, Meredith DM, Mayer PR, Borromeo MD, Lai HC, Ou YH, Johnson JE
Developmental cell25(2)182-95(2013 Apr)

RNA profiling and chromatin immunoprecipitation-sequencing reveal that PTF1a stabilizes pancreas progenitor identity via the control of MNX1/HLXB9 and a network of other transcription factors.
Thompson N, G?sina E, Scheinert P, Bucher P, Grapin-Botton A
Molecular and cellular biology32(6)1189-99(2012 Mar)

Ptf1a directly controls expression of immunoglobulin superfamily molecules Nephrin and Neph3 in the developing central nervous system.
Nishida K, Hoshino M, Kawaguchi Y, Murakami F
The Journal of biological chemistry285(1)373-80(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

combined expression of key genes (Pdx1 and p48) involved in pancreatic development may be a promising approach to generate mature pancreatic exocrine cells.
Title: Viral-mediated coexpression of Pdx1 and p48 regulates exocrine pancreatic differentiation in mouse ES cells.

The ability of truncated versions of Hes1 to bind Ptf1-p48 correlates with their ability to down-regulate the activity of the PTF1 transcriptional complex.
Title: Interactions between hairy/enhancer of split-related proteins and the pancreatic transcription factor Ptf1-p48 modulate function of the PTF1 transcriptional complex.

The following Ptf1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptf1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11194	NM_053964.1 Latest version!	Rattus norvegicus pancreas associated transcription factor 1a (Ptf1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptf1a ( NM_053964.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446416.1 Rattus norvegicus pancreas associated transcription factor 1a (Ptf1a), mRNA.

Price & Availability↑

CloneID	ORa11194
Clone ID Related Accession (Same CDS sequence)	NM_053964.1
Accession Version	NM_053964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	pancreas transcription factor 1 subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X98446.1. On Dec 18, 2003 this sequence version replaced XM_346504.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X98170.1, BE107609.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00632250, SAMN00632251 [ECO:0000348] ##Evidence-Data-END##

ATGGACGCCG TGCTCCTGGA GCATTTTCCC GGGGCCCTGG ACACCTTCCC GTCCTCTTAC 
TTCGATGAGG AAGATTTCTT CACCGATCAG TCCTCTCGGG ACCCACTGGA GGACGGCGAC 
GAGCTGCTGG GGGATGAGCA GGCGGAGGTA GAGTTCCTCA GTCACCAGCT GCACGAATAC 
TGCTACCGCG ACGGGGCGTG CCTGCTGCTG CAACCTGCGC CCTCGGCGGC CCCGCACGCG 
CTCGCTCCGC CGCCTTTGGG GGACCCTGGC GAGCCCGAGG ACAGCGGCAG CTATTGCTGC 
GATGCAGGGG CGCCTCTTGG TGCCTTCCCC TACTCGCCTG GCTCACCGCC CTCCTGTCTC 
GCCTACCCTT GCACCGCGGT ACTGTCCCCC GGTACGCGGC TCCGTGGTTT GAACGGGGCA 
GCGGCAGCGG CGGCGGCGGC CGCAAGGCGG CGGCGACGCG TGCGCTCCGA GGCAGAGCTG 
CAGCAGCTGC GACAAGCGGC CAACGTGCGA GAGCGGCGCC GCATGCAGTC TATCAACGAC 
GCCTTCGAGG GGCTGCGTTC GCACATCCCC ACGCTGCCCT ACGAAAAGCG CCTCTCCAAG 
GTAGACACGC TGCGCTTAGC CATAGGCTAC ATTAACTTCC TCAGCGAGCT GGTGCAAGCC 
GACTTGCCAC TGCGCGGGAG TGGCACAGGT GGTTGCGGGG GCCCAGGTGG CAGCCGGCAC 
CTCGGAGGGG ACAGCCCAGG TAACCAGGCC CAGAAGGTCA TCATCTGCCA TCGAGGCACC 
CGTTCGCCCT CCCCCAGCGA CCCGGACTAC GGTCTCCCTC CTCTTGCAGG GCACTCCCTT 
TCCTGGGCTG ATGAAAAACA GCTCAAAGAA CAAAATATTA TTCGTACAGC TAAAGTGTGG 
ACCCCAGAGG ACCCCAGAAA ACTCAACAGT AAATCTTTTG ACAACATAGA GAACGAACCA 
CCTTTTGAGT TTGTGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446416.1
CDS	234..1214
Translation	MDAVLLEHFPGALDTFPSSYFDEEDFFTDQSSRDPLEDGDELLGDEQAEVEFLSHQLHEYCYRDGACLLLQPAPSAAPHALAPPPLGDPGEPEDSGSYCCDAGAPLGAFPYSPGSPPSCLAYPCTAVLSPGTRLRGLNGAAAAAAAAARRRRRVRSEAELQQLRQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIGYINFLSELVQADLPLRGSGTGGCGGPGGSRHLGGDSPGNQAQKVIICHRGTRSPSPSDPDYGLPPLAGHSLSWADEKQLKEQNIIRTAKVWTPEDPRKLNSKSFDNIENEPPFEFVS

Target ORF information:

RefSeq Version	NM_053964.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pancreas associated transcription factor 1a (Ptf1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053964.1

ORF Insert Sequence:

ATGGACGCCG TGCTCCTGGA GCATTTTCCC GGGGCCCTGG ACACCTTCCC GTCCTCTTAC 
TTCGATGAGG AAGATTTCTT CACCGATCAG TCCTCTCGGG ACCCACTGGA GGACGGCGAC 
GAGCTGCTGG GGGATGAGCA GGCGGAGGTA GAGTTCCTCA GTCACCAGCT GCACGAATAC 
TGCTACCGCG ACGGGGCGTG CCTGCTGCTG CAACCTGCGC CCTCGGCGGC CCCGCACGCG 
CTCGCTCCGC CGCCTTTGGG GGACCCTGGC GAGCCCGAGG ACAGCGGCAG CTATTGCTGC 
GATGCAGGGG CGCCTCTTGG TGCCTTCCCC TACTCGCCTG GCTCACCGCC CTCCTGTCTC 
GCCTACCCTT GCACCGCGGT ACTGTCCCCC GGTACGCGGC TCCGTGGTTT GAACGGGGCA 
GCGGCAGCGG CGGCGGCGGC CGCAAGGCGG CGGCGACGCG TGCGCTCCGA GGCAGAGCTG 
CAGCAGCTGC GACAAGCGGC CAACGTGCGA GAGCGGCGCC GCATGCAGTC TATCAACGAC 
GCCTTCGAGG GGCTGCGTTC GCACATCCCC ACGCTGCCCT ACGAAAAGCG CCTCTCCAAG 
GTAGACACGC TGCGCTTAGC CATAGGCTAC ATTAACTTCC TCAGCGAGCT GGTGCAAGCC 
GACTTGCCAC TGCGCGGGAG TGGCACAGGT GGTTGCGGGG GCCCAGGTGG CAGCCGGCAC 
CTCGGAGGGG ACAGCCCAGG TAACCAGGCC CAGAAGGTCA TCATCTGCCA TCGAGGCACC 
CGTTCGCCCT CCCCCAGCGA CCCGGACTAC GGTCTCCCTC CTCTTGCAGG GCACTCCCTT 
TCCTGGGCTG ATGAAAAACA GCTCAAAGAA CAAAATATTA TTCGTACAGC TAAAGTGTGG 
ACCCCAGAGG ACCCCAGAAA ACTCAACAGT AAATCTTTTG ACAACATAGA GAACGAACCA 
CCTTTTGAGT TTGTGTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nuclear hormone receptor family member NR5A2 controls aspects of multipotent progenitor cell formation and acinar differentiation during pancreatic organogenesis.
Development (Cambridge, England)141(16)3123-33(2014 Aug)
Hale MA,Swift GH,Hoang CQ,Deering TG,Masui T,Lee YK,Xue J,MacDonald RJ

Genome-wide expression analysis of Ptf1a- and Ascl1-deficient mice reveals new markers for distinct dorsal horn interneuron populations contributing to nociceptive reflex plasticity.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(17)7299-307(2013 Apr)
Wildner H,Das Gupta R,Br?hl D,Heppenstall PA,Zeilhofer HU,Birchmeier C

Prdm13 mediates the balance of inhibitory and excitatory neurons in somatosensory circuits.
Developmental cell25(2)182-95(2013 Apr)
Chang JC,Meredith DM,Mayer PR,Borromeo MD,Lai HC,Ou YH,Johnson JE

RNA profiling and chromatin immunoprecipitation-sequencing reveal that PTF1a stabilizes pancreas progenitor identity via the control of MNX1/HLXB9 and a network of other transcription factors.
Molecular and cellular biology32(6)1189-99(2012 Mar)
Thompson N,G?sina E,Scheinert P,Bucher P,Grapin-Botton A

Ptf1a directly controls expression of immunoglobulin superfamily molecules Nephrin and Neph3 in the developing central nervous system.
The Journal of biological chemistry285(1)373-80(2010 Jan)
Nishida K,Hoshino M,Kawaguchi Y,Murakami F

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Ptf1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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