Pip4k2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pip4k2a
Entrez Gene ID	116723
Full Name	phosphatidylinositol-5-phosphate 4-kinase type 2 alpha
Synonyms	Pip5k2a
General protein information	Preferred Names phosphatidylinositol-5-phosphate 4-kinase type 2 alpha Names phosphatidylinositol 5-phosphate 4-kinase type-2 alpha 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha 1-phosphatidylinositol-5-phosphate 4-kinase A PI(5)P 4-kinase type II alpha PIP4KII-alpha PIPK2 alpha diphosphoinositide kinase 2-alpha phosphatidylinositol 5-phosphate 4-kinase type II alpha phosphatidylinositol-4-phosphate 5-kinase, type II, alpha phosphatidylinositol-5-phosphate 4-kinase type-2 alpha-like ptdIns(5)P-4-kinase isoform 2-alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.3
Summary	mouse homolog is kinase which catalyzes the synthesis of phosphatidylinositol 4, 5-bisphosphate, an essential lipid molecule in various cellular processes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053926.2	NP_446378.1	phosphatidylinositol 5-phosphate 4-kinase type-2 alpha

Pathways from BioSystems

Homologies

Homo sapiens (human)	PIP4K2A	NP_005019.2
Canis lupus familiaris (dog)	PIP4K2A	XP_850532.1
Danio rerio (zebrafish)	pip4k2aa	NP_001122174.1
	PIP4K2A	XP_001099389.2
Mus musculus (house mouse)	Pip4k2a	NP_032871.3
Rattus norvegicus (Norway rat)	Pip4k2a	NP_446378.1
Xenopus tropicalis (tropical clawed frog)	pip4k2b	NP_001123723.1
Pan troglodytes (chimpanzee)	PIP4K2A	XP_001145662.2
Bos taurus (cattle)	PIP4K2A	NP_001179698.1
Gallus gallus (chicken)	PIP4K2A	NP_001026142.1

Gene Ontology
Bibliography

Related articles in PubMed

The function of phosphatidylinositol 5-phosphate 4-kinase ? (PI5P4K?) explored using a specific inhibitor that targets the PI5P-binding site.
Clarke JH, Giudici ML, Burke JE, Williams RL, Maloney DJ, Marugan J, Irvine RF
The Biochemical journal466(2)359-67(2015 Mar)

PI(5)P regulates autophagosome biogenesis.
Vicinanza M, Korolchuk VI, Ashkenazi A, Puri C, Menzies FM, Clarke JH, Rubinsztein DC
Molecular cell57(2)219-34(2015 Jan)

Evolutionarily conserved structural changes in phosphatidylinositol 5-phosphate 4-kinase (PI5P4K) isoforms are responsible for differences in enzyme activity and localization.
Clarke JH, Irvine RF
The Biochemical journal454(1)49-57(2013 Aug)

Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesis.
Schulze H, Korpal M, Hurov J, Kim SW, Zhang J, Cantley LC, Graf T, Shivdasani RA
Blood107(10)3868-75(2006 May)

Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family.
Ishihara H, Shibasaki Y, Kizuki N, Wada T, Yazaki Y, Asano T, Oka Y
The Journal of biological chemistry273(15)8741-8(1998 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pip4k2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pip4k2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11080	NM_053926.2 Latest version!	Rattus norvegicus phosphatidylinositol-5-phosphate 4-kinase type 2 alpha (Pip4k2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pip4k2a ( NM_053926.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446378.1 Rattus norvegicus phosphatidylinositol-5-phosphate 4-kinase type 2 alpha (Pip4k2a), mRNA.

Price & Availability↑

CloneID	ORa11080
Clone ID Related Accession (Same CDS sequence)	NM_053926.2
Accession Version	NM_053926.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB032899.1, CV077559.1, CB715008.1, CO573943.1, DV713604.1 and BQ782736.1. On May 23, 2007 this sequence version replaced NM_053926.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGACCC CTAGCAACCT GGGGTCCTCC GTCCTGGCGA GCAAGACCAA GACGAAGAAG 
AAGCACTTCG TGGCTCAGAA AGTGAAGCTG TTCCGGGCCA GCGACCCGCT GCTCAGCGTG 
CTCATGTGGG GGGTCAACCA CTCGATCAAT GAACTGAGCC ATGTTCAAAT CCCTGTCATG 
TTGATGCCTG ATGACTTCAA AGCCTACTCA AAGATAAAGG TGGACAACCA CCTTTTCAAC 
AAGGAAAACA TGCCGAGCCA TTTCAAGTTT AAGGAATACT GCCCGATGGT CTTCCGGAAT 
CTTCGGGAGA GGTTTGGAAT CGACGACCAA GATTTCCAGA ATTCCTTGAC CAGAAGTGCG 
CCCCTTCCCA ATGACTCGCA GGCTCGCAGC GGGGCTCGGT TTCACACGTC CTATGACAAA 
AGATACGTCA TCAAGACCAT TACCAGCGAG GACGTGGCAG AGATGCACAA CATTCTCAAG 
AAGTACCACC AGTATATAGT GGAATGCCAT GGGGTCACCC TCCTTCCTCA GTTCTTGGGA 
ATGTACCGGC TTAATGTCGA CGGAGTGGAA ATATACGTGA TTGTTACGAG GAACGTGTTC 
AGCCACCGTC TCTCTGTGTA TAGGAAGTAC GACCTGAAGG GCTCGACGGT GGCTAGAGAA 
GCTAGTGACA AAGAAAAGGC CAAAGAGCTT CCAACTTTAA AGGACAATGA CTTCATCAAT 
GAGGGCCAAA AGATTTATAT CGATGACAGC AACAAAAAGA TATTCCTGGA AAAACTGAAA 
AAGGATGTCG AGTTCCTTGC ACAACTAAAA CTCATGGACT ACAGCCTCCT GGTGGGGATT 
CATGATGTGG AGCGAGCAGA GCAAGAGGAG GTTGAGTGTG AGGAGAACGA GGGGGAAGAA 
GAAGGAGAGA GTGACGGTGC CCACCCCATC GGGACGCCAC CTGACAGTCC TGGAAACACA 
CTCAACAGCT CACCACCCTT GGCTCCTGGC GAGTTCGACC CAAACATTGA TGTTTATGCA 
ATTAAATGCC ATGAAAACGC ACCTAGGAAA GAAGTGTACT TCATGGCGAT TATTGACATT 
CTCACTCATT ACGATGCAAA AAAGAAAGCT GCCCACGCTG CGAAAACTGT AAAACACGGC 
GCTGGAGCCG AGATCTCGAC CGTGAACCCA GAGCAGTATT CAAAGCGCTT TTTGGACTTT 
ATCGGACACA TCTTGACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446378.1
CDS	97..1317
Translation	MATPSNLGSSVLASKTKTKKKHFVAQKVKLFRASDPLLSVLMWGVNHSINELSHVQIPVMLMPDDFKAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLRERFGIDDQDFQNSLTRSAPLPNDSQARSGARFHTSYDKRYVIKTITSEDVAEMHNILKKYHQYIVECHGVTLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVAREASDKEKAKELPTLKDNDFINEGQKIYIDDSNKKIFLEKLKKDVEFLAQLKLMDYSLLVGIHDVERAEQEEVECEENEGEEEGESDGAHPIGTPPDSPGNTLNSSPPLAPGEFDPNIDVYAIKCHENAPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFLDFIGHILT

Target ORF information:

RefSeq Version	NM_053926.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphatidylinositol-5-phosphate 4-kinase type 2 alpha (Pip4k2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053926.2

ORF Insert Sequence:

ATGGCGACCC CTAGCAACCT GGGGTCCTCC GTCCTGGCGA GCAAGACCAA GACGAAGAAG 
AAGCACTTCG TGGCTCAGAA AGTGAAGCTG TTCCGGGCCA GCGACCCGCT GCTCAGCGTG 
CTCATGTGGG GGGTCAACCA CTCGATCAAT GAACTGAGCC ATGTTCAAAT CCCTGTCATG 
TTGATGCCTG ATGACTTCAA AGCCTACTCA AAGATAAAGG TGGACAACCA CCTTTTCAAC 
AAGGAAAACA TGCCGAGCCA TTTCAAGTTT AAGGAATACT GCCCGATGGT CTTCCGGAAT 
CTTCGGGAGA GGTTTGGAAT CGACGACCAA GATTTCCAGA ATTCCTTGAC CAGAAGTGCG 
CCCCTTCCCA ATGACTCGCA GGCTCGCAGC GGGGCTCGGT TTCACACGTC CTATGACAAA 
AGATACGTCA TCAAGACCAT TACCAGCGAG GACGTGGCAG AGATGCACAA CATTCTCAAG 
AAGTACCACC AGTATATAGT GGAATGCCAT GGGGTCACCC TCCTTCCTCA GTTCTTGGGA 
ATGTACCGGC TTAATGTCGA CGGAGTGGAA ATATACGTGA TTGTTACGAG GAACGTGTTC 
AGCCACCGTC TCTCTGTGTA TAGGAAGTAC GACCTGAAGG GCTCGACGGT GGCTAGAGAA 
GCTAGTGACA AAGAAAAGGC CAAAGAGCTT CCAACTTTAA AGGACAATGA CTTCATCAAT 
GAGGGCCAAA AGATTTATAT CGATGACAGC AACAAAAAGA TATTCCTGGA AAAACTGAAA 
AAGGATGTCG AGTTCCTTGC ACAACTAAAA CTCATGGACT ACAGCCTCCT GGTGGGGATT 
CATGATGTGG AGCGAGCAGA GCAAGAGGAG GTTGAGTGTG AGGAGAACGA GGGGGAAGAA 
GAAGGAGAGA GTGACGGTGC CCACCCCATC GGGACGCCAC CTGACAGTCC TGGAAACACA 
CTCAACAGCT CACCACCCTT GGCTCCTGGC GAGTTCGACC CAAACATTGA TGTTTATGCA 
ATTAAATGCC ATGAAAACGC ACCTAGGAAA GAAGTGTACT TCATGGCGAT TATTGACATT 
CTCACTCATT ACGATGCAAA AAAGAAAGCT GCCCACGCTG CGAAAACTGT AAAACACGGC 
GCTGGAGCCG AGATCTCGAC CGTGAACCCA GAGCAGTATT CAAAGCGCTT TTTGGACTTT 
ATCGGACACA TCTTGACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The function of phosphatidylinositol 5-phosphate 4-kinase ? (PI5P4K?) explored using a specific inhibitor that targets the PI5P-binding site.
The Biochemical journal466(2)359-67(2015 Mar)
Clarke JH,Giudici ML,Burke JE,Williams RL,Maloney DJ,Marugan J,Irvine RF

PI(5)P regulates autophagosome biogenesis.
Molecular cell57(2)219-34(2015 Jan)
Vicinanza M,Korolchuk VI,Ashkenazi A,Puri C,Menzies FM,Clarke JH,Rubinsztein DC

Evolutionarily conserved structural changes in phosphatidylinositol 5-phosphate 4-kinase (PI5P4K) isoforms are responsible for differences in enzyme activity and localization.
The Biochemical journal454(1)49-57(2013 Aug)
Clarke JH,Irvine RF

Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesis.
Blood107(10)3868-75(2006 May)
Schulze H,Korpal M,Hurov J,Kim SW,Zhang J,Cantley LC,Graf T,Shivdasani RA

Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family.
The Journal of biological chemistry273(15)8741-8(1998 Apr)
Ishihara H,Shibasaki Y,Kizuki N,Wada T,Yazaki Y,Asano T,Oka Y

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Pip4k2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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