Cyth1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyth1
Entrez Gene ID	116691
Full Name	cytohesin 1
Synonyms	Pscd1,Sec7
General protein information	Preferred Names cytohesin 1 Names cytohesin-1 PH, SEC7 and coiled-coil domain-containing protein 1 SEC7 homolog A pleckstrin homology, Sec7 and coiled-coil domains 1 pleckstrin homology, Sec7 and coiled/coil domains 1(cytohesin 1) rSec7-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.2-q32.3
Summary	may play a role in transport from the Golgi apparatus to secretory vesicles [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006247784.3	XP_006247846.1	cytohesin-1 isoform X1
NM_053910.1	NP_446362.1	cytohesin-1
XM_006247785.3	XP_006247847.1	cytohesin-1 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cyth1	NP_001106170.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008737	XP_314852.3
Xenopus tropicalis (tropical clawed frog)	cyth2	NP_001005039.1
Homo sapiens (human)	CYTH1	NP_004753.1
Gallus gallus (chicken)	CYTH1	NP_001026276.1
Danio rerio (zebrafish)	cyth1a	NP_999847.1
Canis lupus familiaris (dog)	CYTH1	XP_848819.1
Drosophila melanogaster (fruit fly)	step	NP_610120.1
	CYTH1	XP_001108434.1
Bos taurus (cattle)	CYTH1	XP_002696202.3
Rattus norvegicus (Norway rat)	Cyth1	NP_446362.1
Pan troglodytes (chimpanzee)	CYTH1	XP_511712.2

Gene Ontology
Bibliography

Related articles in PubMed

FRMD4A regulates epithelial polarity by connecting Arf6 activation with the PAR complex.
Ikenouchi J, Umeda M
Proceedings of the National Academy of Sciences of the United States of America107(2)748-53(2010 Jan)

Structural basis and mechanism of autoregulation in 3-phosphoinositide-dependent Grp1 family Arf GTPase exchange factors.
DiNitto JP, Delprato A, Gabe Lee MT, Cronin TC, Huang S, Guilherme A, Czech MP, Lambright DG
Molecular cell28(4)569-83(2007 Nov)

The Arl4 family of small G proteins can recruit the cytohesin Arf6 exchange factors to the plasma membrane.
Hofmann I, Thompson A, Sanderson CM, Munro S
Current biology : CB17(8)711-6(2007 Apr)

Oligomerization controls in tissue-specific manner ligand binding of native, affinity-purified p42(IP4)/centaurin alpha1 and cytohesins-proteins with high affinity for the messengers D-inositol 1,3,4,5-tetrakisphosphate/phosphatidylinositol 3,4,5-trisphosphate.
Stricker R, Vandekerckhove J, Krishna MU, Falck JR, Hanck T, Reiser G
Biochimica et biophysica acta1651(1-2)102-15(2003 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that oligomerization of inositol phosphate/phosphatidylinositol binding proteins such as cytoadhesin 1 and centaurin alpha 1 can modulate their function in a tissue-dependent manner.
Title: Oligomerization controls in tissue-specific manner ligand binding of native, affinity-purified p42(IP4)/centaurin alpha1 and cytohesins-proteins with high affinity for the messengers D-inositol 1,3,4,5-tetrakisphosphate/phosphatidylinositol 3,4,5-trisphosphate.

The following Cyth1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyth1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11030	NM_053910.1 Latest version!	Rattus norvegicus cytohesin 1 (Cyth1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa33960	XM_006247784.3 Latest version!	Rattus norvegicus cytohesin 1 (Cyth1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa33961	XM_006247785.3 Latest version!	Rattus norvegicus cytohesin 1 (Cyth1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyth1 ( NM_053910.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446362.1 Rattus norvegicus cytohesin 1 (Cyth1), mRNA.

Price & Availability↑

CloneID	ORa11030
Clone ID Related Accession (Same CDS sequence)	NM_053910.1
Accession Version	NM_053910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	cytohesin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U83895.1. ##Evidence-Data-START## Transcript exon combination :: U83895.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGACG ATGACAGCTA TGTCCCCAGT GACCTGACTG CAGAGGAGCG TCAAGAACTG 
GAGAACATCC GGCGGAGGAA GCAGGAGCTG CTGGCTGACA TCCAGAGGCT GAAGGAAGAG 
ATAGCAGAGG TTGCTAATGA AATTGAAAGC CTGGGATCCA CAGAGGAACG GAAAAACATG 
CAGAGGAACA AACAGGTAGC CATGGGCAGA AAGAAATTCA ACATGGATCC TAAGAAGGGG 
ATCCAGTTCT TAATCGAGAA TGGCCTGCTG AAAAACACTT GTGAGGACAT TGCCCAGTTC 
CTGTATAAAG GGGAAGGGCT CAACAAAACA GCCATCGGTG ACTACCTTGG GGAGAGAGAT 
GAGTTCAGCA TCCAGGTCTT GCATGCATTT GTGGAGCTGC ACGAGTTCAC CGACCTGAAC 
CTCGTCCAGG CCTTGCGGCA GTTCCTGTGG AGCTTCCGGC TCCCTGGAGA GGCCCAGAAG 
ATTGACCGGA TGATGGAGGC CTTTGCGCAG CGGTACTGTC AGTGCAATAC TGGGGTGTTC 
CAGTCTACAG ACACTTGCTA TGTCCTGTCC TTTGCAATCA TCATGCTGAA CACCAGCCTA 
CACAACCCCA ACGTCAAAGA CAAGCCTACG GTGGAGAGGT TCATCGCCAT GAACCGAGGC 
ATCAATGACG GAGGAGACCT GCCCGAGGAG CTGCTGCGGA ATCTCTATGA GAGCATCAAA 
AACGAACCCT TTAAAATCCC AGAAGATGAC GGCAATGATC TCACTCACAC TTTCTTCAAT 
CCAGACCGAG AGGGCTGGCT ACTAAAGCTC GGAGGTGGCA GGGTAAAGAC TTGGAAGAGG 
CGCTGGTTCA TTCTGACTGA CAACTGCCTT TACTACTTTG AATACACCAC GGACAAAGAG 
CCCCGCGGGA TCATCCCCCT GGAGAACCTG AGTATCCGAG AGGTGGAAGA CTCCAAGAAG 
CCGAACTGCT TTGAGCTTTA TATCCCTGAC AATAAAGACC AGGTGATTAA GGCCTGCAAG 
ACAGAGGCTG ACGGACGAGT GGTGGAAGGG AACCACACTG TGTACCGCAT CTCGGCCCCC 
ACTCCTGAGG AGAAGGAAGA CTGGATCAAG TGCATCAAAG CCGCCATCAG CAGAGACCCG 
TTCTACGAGA TGCTTGCAGC ACGGAAAAAG AAGGTCTCCT CCACAAAGAG GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446362.1
CDS	76..1272
Translation	MEDDDSYVPSDLTAEERQELENIRRRKQELLADIQRLKEEIAEVANEIESLGSTEERKNMQRNKQVAMGRKKFNMDPKKGIQFLIENGLLKNTCEDIAQFLYKGEGLNKTAIGDYLGERDEFSIQVLHAFVELHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCQCNTGVFQSTDTCYVLSFAIIMLNTSLHNPNVKDKPTVERFIAMNRGINDGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDSKKPNCFELYIPDNKDQVIKACKTEADGRVVEGNHTVYRISAPTPEEKEDWIKCIKAAISRDPFYEMLAARKKKVSSTKRH

Target ORF information:

RefSeq Version	NM_053910.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytohesin 1 (Cyth1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053910.1

ORF Insert Sequence:

ATGGAGGACG ATGACAGCTA TGTCCCCAGT GACCTGACTG CAGAGGAGCG TCAAGAACTG 
GAGAACATCC GGCGGAGGAA GCAGGAGCTG CTGGCTGACA TCCAGAGGCT GAAGGAAGAG 
ATAGCAGAGG TTGCTAATGA AATTGAAAGC CTGGGATCCA CAGAGGAACG GAAAAACATG 
CAGAGGAACA AACAGGTAGC CATGGGCAGA AAGAAATTCA ACATGGATCC TAAGAAGGGG 
ATCCAGTTCT TAATCGAGAA TGGCCTGCTG AAAAACACTT GTGAGGACAT TGCCCAGTTC 
CTGTATAAAG GGGAAGGGCT CAACAAAACA GCCATCGGTG ACTACCTTGG GGAGAGAGAT 
GAGTTCAGCA TCCAGGTCTT GCATGCATTT GTGGAGCTGC ACGAGTTCAC CGACCTGAAC 
CTCGTCCAGG CCTTGCGGCA GTTCCTGTGG AGCTTCCGGC TCCCTGGAGA GGCCCAGAAG 
ATTGACCGGA TGATGGAGGC CTTTGCGCAG CGGTACTGTC AGTGCAATAC TGGGGTGTTC 
CAGTCTACAG ACACTTGCTA TGTCCTGTCC TTTGCAATCA TCATGCTGAA CACCAGCCTA 
CACAACCCCA ACGTCAAAGA CAAGCCTACG GTGGAGAGGT TCATCGCCAT GAACCGAGGC 
ATCAATGACG GAGGAGACCT GCCCGAGGAG CTGCTGCGGA ATCTCTATGA GAGCATCAAA 
AACGAACCCT TTAAAATCCC AGAAGATGAC GGCAATGATC TCACTCACAC TTTCTTCAAT 
CCAGACCGAG AGGGCTGGCT ACTAAAGCTC GGAGGTGGCA GGGTAAAGAC TTGGAAGAGG 
CGCTGGTTCA TTCTGACTGA CAACTGCCTT TACTACTTTG AATACACCAC GGACAAAGAG 
CCCCGCGGGA TCATCCCCCT GGAGAACCTG AGTATCCGAG AGGTGGAAGA CTCCAAGAAG 
CCGAACTGCT TTGAGCTTTA TATCCCTGAC AATAAAGACC AGGTGATTAA GGCCTGCAAG 
ACAGAGGCTG ACGGACGAGT GGTGGAAGGG AACCACACTG TGTACCGCAT CTCGGCCCCC 
ACTCCTGAGG AGAAGGAAGA CTGGATCAAG TGCATCAAAG CCGCCATCAG CAGAGACCCG 
TTCTACGAGA TGCTTGCAGC ACGGAAAAAG AAGGTCTCCT CCACAAAGAG GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyth1 ( XM_006247784.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247846.1 Rattus norvegicus cytohesin 1 (Cyth1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33960
Clone ID Related Accession (Same CDS sequence)	XM_006247784.3
Accession Version	XM_006247784.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytohesin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247784.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG ATGACAGCTA TGTCCCCAGT GACCTGACTG CAGAGGAGCG TCAAGAACTG 
GAGAACATCC GGCGGAGGAA GCAGGAGCTG CTGGCTGACA TCCAGAGGCT GAAGGAAGAG 
ATAGCAGAGG TTGCTAATGA AATTGAAAGC CTGGGATCCA CAGAGGAACG GAAAAACATG 
CAGAGGAACA AACAGGTAGC CATGGGCAGA AAGAAATTCA ACATGGATCC TAAGAAGGGG 
ATCCAGTTCT TAATCGAGAA TGGCCTGCTG AAAAACACTT GTGAGGACAT TGCCCAGTTC 
CTGTATAAAG GGGAAGGGCT CAACAAAACA GCCATCGGTG ACTACCTTGG GGAGAGAGAT 
GAGTTCAGCA TCCAGGTCTT GCATGCATTT GTGGAGCTGC ACGAGTTCAC CGACCTGAAC 
CTCGTCCAGG CCTTGCGGCA GTTCCTGTGG AGCTTCCGGC TCCCTGGAGA GGCCCAGAAG 
ATTGACCGGA TGATGGAGGC CTTTGCGCAG CGGTACTGTC AGTGCAATAC TGGGGTGTTC 
CAGTCTACAG ACACTTGCTA TGTCCTGTCC TTTGCAATCA TCATGCTGAA CACCAGCCTA 
CACAACCCCA ACGTCAAAGA CAAGCCTACG GTGGAGAGGT TCATCGCCAT GAACCGAGGC 
ATCAATGACG GAGGAGACCT GCCCGAGGAG CTGCTGCGGA ATCTCTATGA GAGCATCAAA 
AACGAACCCT TTAAAATCCC AGAAGATGAC GGCAATGATC TCACTCACAC TTTCTTCAAT 
CCAGACCGAG AGGGCTGGCT ACTAAAGCTC GGTGGCAGGG TAAAGACTTG GAAGAGGCGC 
TGGTTCATTC TGACTGACAA CTGCCTTTAC TACTTTGAAT ACACCACGGA CAAAGAGCCC 
CGCGGGATCA TCCCCCTGGA GAACCTGAGT ATCCGAGAGG TGGAAGACTC CAAGAAGCCG 
AACTGCTTTG AGCTTTATAT CCCTGACAAT AAAGACCAGG TGATTAAGGC CTGCAAGACA 
GAGGCTGACG GACGAGTGGT GGAAGGGAAC CACACTGTGT ACCGCATCTC GGCCCCCACT 
CCTGAGGAGA AGGAAGACTG GATCAAGTGC ATCAAAGCCG CCATCAGCAG AGACCCGTTC 
TACGAGATGC TTGCAGCACG GAAAAAGAAG GTCTCCTCCA CAAAGAGGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247846.1
CDS	126..1319
Translation	MEDDDSYVPSDLTAEERQELENIRRRKQELLADIQRLKEEIAEVANEIESLGSTEERKNMQRNKQVAMGRKKFNMDPKKGIQFLIENGLLKNTCEDIAQFLYKGEGLNKTAIGDYLGERDEFSIQVLHAFVELHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCQCNTGVFQSTDTCYVLSFAIIMLNTSLHNPNVKDKPTVERFIAMNRGINDGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDSKKPNCFELYIPDNKDQVIKACKTEADGRVVEGNHTVYRISAPTPEEKEDWIKCIKAAISRDPFYEMLAARKKKVSSTKRH

Target ORF information:

RefSeq Version	XM_006247784.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytohesin 1 (Cyth1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247784.3

ORF Insert Sequence:

ATGGAGGACG ATGACAGCTA TGTCCCCAGT GACCTGACTG CAGAGGAGCG TCAAGAACTG 
GAGAACATCC GGCGGAGGAA GCAGGAGCTG CTGGCTGACA TCCAGAGGCT GAAGGAAGAG 
ATAGCAGAGG TTGCTAATGA AATTGAAAGC CTGGGATCCA CAGAGGAACG GAAAAACATG 
CAGAGGAACA AACAGGTAGC CATGGGCAGA AAGAAATTCA ACATGGATCC TAAGAAGGGG 
ATCCAGTTCT TAATCGAGAA TGGCCTGCTG AAAAACACTT GTGAGGACAT TGCCCAGTTC 
CTGTATAAAG GGGAAGGGCT CAACAAAACA GCCATCGGTG ACTACCTTGG GGAGAGAGAT 
GAGTTCAGCA TCCAGGTCTT GCATGCATTT GTGGAGCTGC ACGAGTTCAC CGACCTGAAC 
CTCGTCCAGG CCTTGCGGCA GTTCCTGTGG AGCTTCCGGC TCCCTGGAGA GGCCCAGAAG 
ATTGACCGGA TGATGGAGGC CTTTGCGCAG CGGTACTGTC AGTGCAATAC TGGGGTGTTC 
CAGTCTACAG ACACTTGCTA TGTCCTGTCC TTTGCAATCA TCATGCTGAA CACCAGCCTA 
CACAACCCCA ACGTCAAAGA CAAGCCTACG GTGGAGAGGT TCATCGCCAT GAACCGAGGC 
ATCAATGACG GAGGAGACCT GCCCGAGGAG CTGCTGCGGA ATCTCTATGA GAGCATCAAA 
AACGAACCCT TTAAAATCCC AGAAGATGAC GGCAATGATC TCACTCACAC TTTCTTCAAT 
CCAGACCGAG AGGGCTGGCT ACTAAAGCTC GGTGGCAGGG TAAAGACTTG GAAGAGGCGC 
TGGTTCATTC TGACTGACAA CTGCCTTTAC TACTTTGAAT ACACCACGGA CAAAGAGCCC 
CGCGGGATCA TCCCCCTGGA GAACCTGAGT ATCCGAGAGG TGGAAGACTC CAAGAAGCCG 
AACTGCTTTG AGCTTTATAT CCCTGACAAT AAAGACCAGG TGATTAAGGC CTGCAAGACA 
GAGGCTGACG GACGAGTGGT GGAAGGGAAC CACACTGTGT ACCGCATCTC GGCCCCCACT 
CCTGAGGAGA AGGAAGACTG GATCAAGTGC ATCAAAGCCG CCATCAGCAG AGACCCGTTC 
TACGAGATGC TTGCAGCACG GAAAAAGAAG GTCTCCTCCA CAAAGAGGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyth1 ( XM_006247785.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247847.1 Rattus norvegicus cytohesin 1 (Cyth1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa33961
Clone ID Related Accession (Same CDS sequence)	XM_006247785.3
Accession Version	XM_006247785.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytohesin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247785.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGGA ACAAACAGGT AGCCATGGGC AGAAAGAAAT TCAACATGGA TCCTAAGAAG 
GGGATCCAGT TCTTAATCGA GAATGGCCTG CTGAAAAACA CTTGTGAGGA CATTGCCCAG 
TTCCTGTATA AAGGGGAAGG GCTCAACAAA ACAGCCATCG GTGACTACCT TGGGGAGAGA 
GATGAGTTCA GCATCCAGGT CTTGCATGCA TTTGTGGAGC TGCACGAGTT CACCGACCTG 
AACCTCGTCC AGGCCTTGCG GCAGTTCCTG TGGAGCTTCC GGCTCCCTGG AGAGGCCCAG 
AAGATTGACC GGATGATGGA GGCCTTTGCG CAGCGGTACT GTCAGTGCAA TACTGGGGTG 
TTCCAGTCTA CAGACACTTG CTATGTCCTG TCCTTTGCAA TCATCATGCT GAACACCAGC 
CTACACAACC CCAACGTCAA AGACAAGCCT ACGGTGGAGA GGTTCATCGC CATGAACCGA 
GGCATCAATG ACGGAGGAGA CCTGCCCGAG GAGCTGCTGC GGAATCTCTA TGAGAGCATC 
AAAAACGAAC CCTTTAAAAT CCCAGAAGAT GACGGCAATG ATCTCACTCA CACTTTCTTC 
AATCCAGACC GAGAGGGCTG GCTACTAAAG CTCGGAGGTG GCAGGGTAAA GACTTGGAAG 
AGGCGCTGGT TCATTCTGAC TGACAACTGC CTTTACTACT TTGAATACAC CACGGACAAA 
GAGCCCCGCG GGATCATCCC CCTGGAGAAC CTGAGTATCC GAGAGGTGGA AGACTCCAAG 
AAGCCGAACT GCTTTGAGCT TTATATCCCT GACAATAAAG ACCAGGTGAT TAAGGCCTGC 
AAGACAGAGG CTGACGGACG AGTGGTGGAA GGGAACCACA CTGTGTACCG CATCTCGGCC 
CCCACTCCTG AGGAGAAGGA AGACTGGATC AAGTGCATCA AAGCCGCCAT CAGCAGAGAC 
CCGTTCTACG AGATGCTTGC AGCACGGAAA AAGAAGGTCT CCTCCACAAA GAGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247847.1
CDS	1387..2406
Translation	MQRNKQVAMGRKKFNMDPKKGIQFLIENGLLKNTCEDIAQFLYKGEGLNKTAIGDYLGERDEFSIQVLHAFVELHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCQCNTGVFQSTDTCYVLSFAIIMLNTSLHNPNVKDKPTVERFIAMNRGINDGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDSKKPNCFELYIPDNKDQVIKACKTEADGRVVEGNHTVYRISAPTPEEKEDWIKCIKAAISRDPFYEMLAARKKKVSSTKRH

Target ORF information:

RefSeq Version	XM_006247785.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytohesin 1 (Cyth1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247785.3

ORF Insert Sequence:

ATGCAGAGGA ACAAACAGGT AGCCATGGGC AGAAAGAAAT TCAACATGGA TCCTAAGAAG 
GGGATCCAGT TCTTAATCGA GAATGGCCTG CTGAAAAACA CTTGTGAGGA CATTGCCCAG 
TTCCTGTATA AAGGGGAAGG GCTCAACAAA ACAGCCATCG GTGACTACCT TGGGGAGAGA 
GATGAGTTCA GCATCCAGGT CTTGCATGCA TTTGTGGAGC TGCACGAGTT CACCGACCTG 
AACCTCGTCC AGGCCTTGCG GCAGTTCCTG TGGAGCTTCC GGCTCCCTGG AGAGGCCCAG 
AAGATTGACC GGATGATGGA GGCCTTTGCG CAGCGGTACT GTCAGTGCAA TACTGGGGTG 
TTCCAGTCTA CAGACACTTG CTATGTCCTG TCCTTTGCAA TCATCATGCT GAACACCAGC 
CTACACAACC CCAACGTCAA AGACAAGCCT ACGGTGGAGA GGTTCATCGC CATGAACCGA 
GGCATCAATG ACGGAGGAGA CCTGCCCGAG GAGCTGCTGC GGAATCTCTA TGAGAGCATC 
AAAAACGAAC CCTTTAAAAT CCCAGAAGAT GACGGCAATG ATCTCACTCA CACTTTCTTC 
AATCCAGACC GAGAGGGCTG GCTACTAAAG CTCGGAGGTG GCAGGGTAAA GACTTGGAAG 
AGGCGCTGGT TCATTCTGAC TGACAACTGC CTTTACTACT TTGAATACAC CACGGACAAA 
GAGCCCCGCG GGATCATCCC CCTGGAGAAC CTGAGTATCC GAGAGGTGGA AGACTCCAAG 
AAGCCGAACT GCTTTGAGCT TTATATCCCT GACAATAAAG ACCAGGTGAT TAAGGCCTGC 
AAGACAGAGG CTGACGGACG AGTGGTGGAA GGGAACCACA CTGTGTACCG CATCTCGGCC 
CCCACTCCTG AGGAGAAGGA AGACTGGATC AAGTGCATCA AAGCCGCCAT CAGCAGAGAC 
CCGTTCTACG AGATGCTTGC AGCACGGAAA AAGAAGGTCT CCTCCACAAA GAGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FRMD4A regulates epithelial polarity by connecting Arf6 activation with the PAR complex.
Proceedings of the National Academy of Sciences of the United States of America107(2)748-53(2010 Jan)
Ikenouchi J,Umeda M

Structural basis and mechanism of autoregulation in 3-phosphoinositide-dependent Grp1 family Arf GTPase exchange factors.
Molecular cell28(4)569-83(2007 Nov)
DiNitto JP,Delprato A,Gabe Lee MT,Cronin TC,Huang S,Guilherme A,Czech MP,Lambright DG

The Arl4 family of small G proteins can recruit the cytohesin Arf6 exchange factors to the plasma membrane.
Current biology : CB17(8)711-6(2007 Apr)
Hofmann I,Thompson A,Sanderson CM,Munro S

Oligomerization controls in tissue-specific manner ligand binding of native, affinity-purified p42(IP4)/centaurin alpha1 and cytohesins-proteins with high affinity for the messengers D-inositol 1,3,4,5-tetrakisphosphate/phosphatidylinositol 3,4,5-trisphosphate.
Biochimica et biophysica acta1651(1-2)102-15(2003 Sep)
Stricker R,Vandekerckhove J,Krishna MU,Falck JR,Hanck T,Reiser G

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Cyth1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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