Oplah cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Oplah
Entrez Gene ID	116684
Full Name	5-oxoprolinase (ATP-hydrolysing)
General protein information	Preferred Names 5-oxoprolinase (ATP-hydrolysing) Names 5-oxoprolinase 5-OPase 5-oxo-L-prolinase pyroglutamase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34
Summary	catalyzes cleavage of 5-oxo-L-proline to form L-glutamate and is coupled to the hydrolysis of ATP to ADP and inorganic phosphate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006241774.3	XP_006241836.1	5-oxoprolinase isoform X4
XM_017594576.1	XP_017450065.1	5-oxoprolinase isoform X4
NM_053904.1	NP_446356.1	5-oxoprolinase
XM_006241772.3	XP_006241834.1	5-oxoprolinase isoform X2
XM_017594577.1	XP_017450066.1	5-oxoprolinase isoform X4
XM_006241773.3	XP_006241835.1	5-oxoprolinase isoform X3
XM_006241775.3	XP_006241837.1	5-oxoprolinase isoform X4
XM_006241771.3	XP_006241833.1	5-oxoprolinase isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	oplah	NP_001072883.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001606	XP_321495.3
Eremothecium gossypii	AGOS_ABR114C	NP_983061.1
Mus musculus (house mouse)	Oplah	NP_694762.1
Danio rerio (zebrafish)	si:dkey-9l5.2	NP_001186988.1
Magnaporthe oryzae (rice blast fungus)	MGG_00771	XP_003718224.1
Pan troglodytes (chimpanzee)	OPLAH	XP_003951428.1
Rattus norvegicus (Norway rat)	Oplah	NP_446356.1
Drosophila melanogaster (fruit fly)	CG4752	NP_611675.2
Caenorhabditis elegans (roundworm)	Y38F2AR.12	NP_001076725.1
Saccharomyces cerevisiae (baker's yeast)	OXP1	NP_012707.1
Kluyveromyces lactis	KLLA0C18975g	XP_453046.1
Arabidopsis thaliana (thale cress)	OXP1	NP_198599.1
Homo sapiens (human)	OPLAH	NP_060040.1
Canis lupus familiaris (dog)	OPLAH	XP_005628367.1
Bos taurus (cattle)	OPLAH	NP_001001173.1
Schizosaccharomyces pombe (fission yeast)	SPAC11D3.15	NP_592810.1
Neurospora crassa	NCU04569	XP_958668.2

Gene Ontology
Bibliography

Related articles in PubMed

The amino acid sequence of rat kidney 5-oxo-L-prolinase determined by cDNA cloning.
Ye GJ, Breslow EB, Meister A, Guo-jie GE
The Journal of biological chemistry271(50)32293-300(1996 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Oplah gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Oplah cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa30436	XM_006241775.3 Latest version!	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
ORa30436	XM_017594577.1 Latest version!	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
ORa30436	XM_006241774.3 Latest version!	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
ORa30436	XM_017594576.1 Latest version!	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
ORa11013	NM_053904.1 Latest version!	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$944.30 $1349.00
ORa30433	XM_006241771.3 Latest version!	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa30434	XM_006241772.3 Latest version!	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa30435	XM_006241773.3 Latest version!	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Oplah ( XM_006241774.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241836.1 Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa30436
Clone ID Related Accession (Same CDS sequence)	XM_006241774.3 , XM_017594576.1 , XM_017594577.1 , XM_006241775.3
Accession Version	XM_006241774.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	5-oxoprolinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241774.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCC CAGAAGGGCG CTTCCATTTC GCCATCGACC GCGGTGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCTGG AGGGCATGTG CGTGTCCTGA AGCTGCTCTC AGAGGACCCT 
GCCAACTATG CAGATGCACC CACAGAGGGC ATCCGCCGAA TTCTAGAGCA GGAGGAGGGT 
GTGCTGCTGC CTCGAGGCCG ACCGCTAGAC ACCAGTCGCA TTGCCAGCAT CCGCATGGGT 
ACCACGGTGG CCACCAATGC ACTGTTGGAA CGACAGGGAG AACGGGTGGC ACTGCTGGTG 
ACTCGGGGTT TCCGAGACCT GCTGCATATT GGCACTCAGG CCCGCCCGGA CCTCTTTGAC 
TTGGCTGTGC CCATGCCAGA GGTTCTGTAT GAGGAAGTGC TGGAGGTAGA TGAGCGAGTG 
GTGCTGTATC GCGGAGAACC AGGTGCCGGC TCTCCTGTCA AAGGCCGCAC AGGGGACCTG 
CTAGAGATAC AGCAGCCTGT GGACCTGGAA GCCCTGCGTG GGAAGCTGGA GGGGCTCTTG 
TCTCGGGGCA TTCACAGTCT GGCAGTGGTG CTCATGCATT CGTACACGTG GGCCCAGCAT 
GAGCAGCAGG TGGGCACGCT GGCCCGGGAG CTGGGCTTCA CGCACGTCTC CTTGTCCTCG 
GAAGTCATGC CCATGGTACG AATTGTTCCT CGGGGCCATA CAGCCTGTGC TGACGCTTAC 
CTTACTCCCA CCATCCAGCG CTATGTGCAG GGCTTCCGCC GAGGCTTCCA GGGCCAGCTA 
AAGAATGTGC AAGTTCTCTT CATGCGCTCT GATGGTGGCC TCGCACCCAT GGATGCTTTC 
AGTGGTTCCC GGGCTGTGCT CTCTGGCCCT GCTGGGGGTG TGGTTGGCTA CTCAGCTACC 
ACCTACCATC TGGAAGGCGG TCAGCCTGTC ATTGGCTTTG ACATGGGAGG CACATCCACA 
GACGTGAGCC GCTATGCTGG AGAATTTGAG CATGTCTTTG AGGCTAGCAC AGCAGGCGTT 
ACCCTTCAGG CACCCCAGTT GGACATCAAC ACAGTGGCAG CTGGCGGGGG TTCCCGCCTC 
TTCTTCAGAT CTGGCCTCTT TGTGGTTGGT CCAGAGTCAG CAGGTGCCCA CCCAGGTCCT 
GCCTGCTACC GTAAAGGGGG TCCTGTGACA GTGACAGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCAGGAG AAGACCAGCC ACTGTCTCCT 
GAGGCTTCCC GAAAGGCTCT AGAGGCTGTG GCCATGGAGG TCAACAGTTT CTTGACCAAT 
GGACCGTGCC CAGCTTCCCA ACTAAGTCTG GAAGAGGTGG CCATGGGGTT TGTGCGTGTT 
GCCAATGAAG CCATGTGCCG GCCTATCCGT GCCCTCACAC AGGCACGAGG CCATGACCCC 
TCAGCCCATG TATTGGCTTG CTTTGGAGGA GCTGGTGGGC AACACGCTTG TGCCATTGCC 
CGGGCCCTGG GGATGGATAC TGTGCACATT CACAGGCACA GCGGGCTGCT GTCAGCACTA 
GGACTGGCCT TGGCAGATGT GGTTCACGAA GCACAGGAGC CCTGTTCCCT GTCTTACACA 
CCTGAAACCT TTGCACAACT GGACCAGAGA CTGAGCCGCC TGGAGGAGCA GTGTGTGGAT 
GCCTTGCAGG TCCAGGGCTT CCCTAGGTCT CAGATCAGCA CCGAGAGCTT CCTGCATCTT 
CGCTACCAAG GCACTGACTG CGCCCTAATG GTGTCTGCCC ATCAGCATCC GGCCACAGCC 
TGCTCACCCC GTGCTGGTGA CTTTGGAGCC GCATTTGTGG AGAGGTACAT GAGAGAGTTT 
GGCTTCATTA TCCCCGAGCG GCCGGTGGTG GTAGATGATG TACGTGTGAG GGGAACTGGC 
CGTAGTGGAC TTCAGCTGGA GGACACCCCC AAAATCCAGA CTGGACCTCC CCACGTGGAA 
AAGGTGACCC AGTGCTACTT TGAAGGGGGT TATCAGGAGA CTCCCGTGTA CCTTTTAGGA 
GAACTAGGCT ACGGGCACCA GCTCCAAGGG CCCTGCCTTA TCATCGACAA CAACAGCACC 
ATCCTTGTAG AACCGGGTTG CCAAGCAGAG GTGACTGATA CAGGGGACAT CCGCATTTCT 
GTGGGAGCTG AGGGTCCTAG TATGGCAGAT ACCAGGCTTG ACCCCATCCA GCTGTCTATT 
TTCTCACACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTACA GCGCACAGCC 
ATCTCTACCA ACATCAAGGA ACGCCTCGAC TTCTCCTGTG CCCTCTTTGG GCCAGATGGG 
GGCCTCGTCT CCAATGCTCC CCACATTCCT GTGCACCTGG GTGCCATGCA AGAGACTGTA 
CAGTTCCAGA TTCAGCACTT AGGAGCCGAC CTCCATCCTG GTGATGTGTT GCTCAGCAAC 
CATCCCAGCG CAGGGGGCAG CCATCTTCCT GACCTGACTG TCATTACACC GGTGTTTTGG 
CCAGGCCAGA CGAGGCCTGT GTTCTACGTG GCTAGCCGAG GGCACCACGC AGACATTGGA 
GGAATCACAC CGGGCTCTAT GCCGCCTCAC TCCACCACGC TGCAACAGGA GGGTGCCGTT 
TTTCTGTCCT TCAAACTGGT CCAGGGAGGC GTCTTCCAGG AAGAGGCAGT GACAGAGGCC 
CTACGGGCAC CAGGCAAGAT CTCTGGCTGT AGTGGAACCA GGAACCTGCA TGACAACCTG 
TCGGATCTTC GTGCCCAGGT GGCAGCTAAC CAGAAAGGCA TCCAGCTGGT GGGAGAGCTG 
ATCGGACAGT ATGGCTTAGA TGTGGTGCAG GCCTATATGG GCCATATTCA GGCGAATGCT 
GAGCTAGCAG TGAGAGACAT GCTCCGGGCT TTTGGAACTT CCCGGCAGGC CAGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGATCACATG GATGATGGCT CTCCCATCTG TCTGCGTGTT 
CAGATCAACC TGAGTCAGGG CAGTGCGGTA TTTGACTTCA CTGGTTCCGG GTCTGAGGTG 
TTTGGCAATC TCAATGCCCC GAGAGCCATA ACACTGTCTG CTCTCATCTA TTGCTTACGC 
TGTCTAGTGG GCCGTGACAT CCCACTTAAC CAGGGTTGCC TGGCTCCTGT GCGTGTCATA 
ATTCCCAAAG GCTCCATATT GGATCCATCC CCAGAGGCAG CAGTGGTCGG CGGCAACGTG 
CTCACATCTC AGCGAGTAGT GGATGTCATT CTGGGGGCTT TTGGGGCCTG TTCAGCCTCC 
CAGGGCTGCA TGAACAATGT GACCCTGGGC AATGCCCGTA TGGGCTACTA TGAGACAGTG 
GCTGGTGGTG CCGGTGCGGG CCCTGGCTGG CATGGGCGCA GTGGTGTACA CAGTCACATG 
ACCAACACAC GCATTACGGA TCCAGAGATT CTGGAGAGTC GGTATCCAGT TATCCTGCGC 
CGCTTTGAGC TGAGGCCAGG CTCCGGGGGC CGAGGTCGCT TCCGGGGAGG TGATGGCGTA 
GTCCGAGAGC TGGTCTTTCG GGAAGAGGCG CTGTTGTCTG TGCTCACCGA GCGCCGGGCC 
TTCCAGCCTT ACGGCCTCCA CGGGGGAGAG CCTGGTGCGC GTGGCTTAAA CCTCCTGATC 
AGAAAAGATG GGCGCACAGT GAATTTGGGC GGCAAGACAT CTGTGACCGT GTACCCCGGG 
GACGTGTTCT GCCTCCACAC GCCTGGGGGT GGGGGCTACG GAGACCCGGA GGATCCAGCG 
CCACCACCAG GCTCGCCCCC GCTATTTCCA GCCTTCCCCG AGCGCGGCAG TGTATTCGAG 
TACCGCCGCG CCCAGGAAGC CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241836.1
CDS	443..4309
Translation	MGSPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRILEQEEGVLLPRGRPLDTSRIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDLFDLAVPMPEVLYEEVLEVDERVVLYRGEPGAGSPVKGRTGDLLEIQQPVDLEALRGKLEGLLSRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSLSSEVMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAFSGSRAVLSGPAGGVVGYSATTYHLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVAMEVNSFLTNGPCPASQLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLSYTPETFAQLDQRLSRLEEQCVDALQVQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPATACSPRAGDFGAAFVERYMREFGFIIPERPVVVDDVRVRGTGRSGLQLEDTPKIQTGPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNSTILVEPGCQAEVTDTGDIRISVGAEGPSMADTRLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKISGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGLPLEVSAEDHMDDGSPICLRVQINLSQGSAVFDFTGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVIIPKGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACSASQGCMNNVTLGNARMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELVFREEALLSVLTERRAFQPYGLHGGEPGARGLNLLIRKDGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPAPPPGSPPLFPAFPERGSVFEYRRAQEAV

Target ORF information:

RefSeq Version	XM_006241774.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241774.3

ORF Insert Sequence:

ATGGGCAGCC CAGAAGGGCG CTTCCATTTC GCCATCGACC GCGGTGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCTGG AGGGCATGTG CGTGTCCTGA AGCTGCTCTC AGAGGACCCT 
GCCAACTATG CAGATGCACC CACAGAGGGC ATCCGCCGAA TTCTAGAGCA GGAGGAGGGT 
GTGCTGCTGC CTCGAGGCCG ACCGCTAGAC ACCAGTCGCA TTGCCAGCAT CCGCATGGGT 
ACCACGGTGG CCACCAATGC ACTGTTGGAA CGACAGGGAG AACGGGTGGC ACTGCTGGTG 
ACTCGGGGTT TCCGAGACCT GCTGCATATT GGCACTCAGG CCCGCCCGGA CCTCTTTGAC 
TTGGCTGTGC CCATGCCAGA GGTTCTGTAT GAGGAAGTGC TGGAGGTAGA TGAGCGAGTG 
GTGCTGTATC GCGGAGAACC AGGTGCCGGC TCTCCTGTCA AAGGCCGCAC AGGGGACCTG 
CTAGAGATAC AGCAGCCTGT GGACCTGGAA GCCCTGCGTG GGAAGCTGGA GGGGCTCTTG 
TCTCGGGGCA TTCACAGTCT GGCAGTGGTG CTCATGCATT CGTACACGTG GGCCCAGCAT 
GAGCAGCAGG TGGGCACGCT GGCCCGGGAG CTGGGCTTCA CGCACGTCTC CTTGTCCTCG 
GAAGTCATGC CCATGGTACG AATTGTTCCT CGGGGCCATA CAGCCTGTGC TGACGCTTAC 
CTTACTCCCA CCATCCAGCG CTATGTGCAG GGCTTCCGCC GAGGCTTCCA GGGCCAGCTA 
AAGAATGTGC AAGTTCTCTT CATGCGCTCT GATGGTGGCC TCGCACCCAT GGATGCTTTC 
AGTGGTTCCC GGGCTGTGCT CTCTGGCCCT GCTGGGGGTG TGGTTGGCTA CTCAGCTACC 
ACCTACCATC TGGAAGGCGG TCAGCCTGTC ATTGGCTTTG ACATGGGAGG CACATCCACA 
GACGTGAGCC GCTATGCTGG AGAATTTGAG CATGTCTTTG AGGCTAGCAC AGCAGGCGTT 
ACCCTTCAGG CACCCCAGTT GGACATCAAC ACAGTGGCAG CTGGCGGGGG TTCCCGCCTC 
TTCTTCAGAT CTGGCCTCTT TGTGGTTGGT CCAGAGTCAG CAGGTGCCCA CCCAGGTCCT 
GCCTGCTACC GTAAAGGGGG TCCTGTGACA GTGACAGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCAGGAG AAGACCAGCC ACTGTCTCCT 
GAGGCTTCCC GAAAGGCTCT AGAGGCTGTG GCCATGGAGG TCAACAGTTT CTTGACCAAT 
GGACCGTGCC CAGCTTCCCA ACTAAGTCTG GAAGAGGTGG CCATGGGGTT TGTGCGTGTT 
GCCAATGAAG CCATGTGCCG GCCTATCCGT GCCCTCACAC AGGCACGAGG CCATGACCCC 
TCAGCCCATG TATTGGCTTG CTTTGGAGGA GCTGGTGGGC AACACGCTTG TGCCATTGCC 
CGGGCCCTGG GGATGGATAC TGTGCACATT CACAGGCACA GCGGGCTGCT GTCAGCACTA 
GGACTGGCCT TGGCAGATGT GGTTCACGAA GCACAGGAGC CCTGTTCCCT GTCTTACACA 
CCTGAAACCT TTGCACAACT GGACCAGAGA CTGAGCCGCC TGGAGGAGCA GTGTGTGGAT 
GCCTTGCAGG TCCAGGGCTT CCCTAGGTCT CAGATCAGCA CCGAGAGCTT CCTGCATCTT 
CGCTACCAAG GCACTGACTG CGCCCTAATG GTGTCTGCCC ATCAGCATCC GGCCACAGCC 
TGCTCACCCC GTGCTGGTGA CTTTGGAGCC GCATTTGTGG AGAGGTACAT GAGAGAGTTT 
GGCTTCATTA TCCCCGAGCG GCCGGTGGTG GTAGATGATG TACGTGTGAG GGGAACTGGC 
CGTAGTGGAC TTCAGCTGGA GGACACCCCC AAAATCCAGA CTGGACCTCC CCACGTGGAA 
AAGGTGACCC AGTGCTACTT TGAAGGGGGT TATCAGGAGA CTCCCGTGTA CCTTTTAGGA 
GAACTAGGCT ACGGGCACCA GCTCCAAGGG CCCTGCCTTA TCATCGACAA CAACAGCACC 
ATCCTTGTAG AACCGGGTTG CCAAGCAGAG GTGACTGATA CAGGGGACAT CCGCATTTCT 
GTGGGAGCTG AGGGTCCTAG TATGGCAGAT ACCAGGCTTG ACCCCATCCA GCTGTCTATT 
TTCTCACACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTACA GCGCACAGCC 
ATCTCTACCA ACATCAAGGA ACGCCTCGAC TTCTCCTGTG CCCTCTTTGG GCCAGATGGG 
GGCCTCGTCT CCAATGCTCC CCACATTCCT GTGCACCTGG GTGCCATGCA AGAGACTGTA 
CAGTTCCAGA TTCAGCACTT AGGAGCCGAC CTCCATCCTG GTGATGTGTT GCTCAGCAAC 
CATCCCAGCG CAGGGGGCAG CCATCTTCCT GACCTGACTG TCATTACACC GGTGTTTTGG 
CCAGGCCAGA CGAGGCCTGT GTTCTACGTG GCTAGCCGAG GGCACCACGC AGACATTGGA 
GGAATCACAC CGGGCTCTAT GCCGCCTCAC TCCACCACGC TGCAACAGGA GGGTGCCGTT 
TTTCTGTCCT TCAAACTGGT CCAGGGAGGC GTCTTCCAGG AAGAGGCAGT GACAGAGGCC 
CTACGGGCAC CAGGCAAGAT CTCTGGCTGT AGTGGAACCA GGAACCTGCA TGACAACCTG 
TCGGATCTTC GTGCCCAGGT GGCAGCTAAC CAGAAAGGCA TCCAGCTGGT GGGAGAGCTG 
ATCGGACAGT ATGGCTTAGA TGTGGTGCAG GCCTATATGG GCCATATTCA GGCGAATGCT 
GAGCTAGCAG TGAGAGACAT GCTCCGGGCT TTTGGAACTT CCCGGCAGGC CAGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGATCACATG GATGATGGCT CTCCCATCTG TCTGCGTGTT 
CAGATCAACC TGAGTCAGGG CAGTGCGGTA TTTGACTTCA CTGGTTCCGG GTCTGAGGTG 
TTTGGCAATC TCAATGCCCC GAGAGCCATA ACACTGTCTG CTCTCATCTA TTGCTTACGC 
TGTCTAGTGG GCCGTGACAT CCCACTTAAC CAGGGTTGCC TGGCTCCTGT GCGTGTCATA 
ATTCCCAAAG GCTCCATATT GGATCCATCC CCAGAGGCAG CAGTGGTCGG CGGCAACGTG 
CTCACATCTC AGCGAGTAGT GGATGTCATT CTGGGGGCTT TTGGGGCCTG TTCAGCCTCC 
CAGGGCTGCA TGAACAATGT GACCCTGGGC AATGCCCGTA TGGGCTACTA TGAGACAGTG 
GCTGGTGGTG CCGGTGCGGG CCCTGGCTGG CATGGGCGCA GTGGTGTACA CAGTCACATG 
ACCAACACAC GCATTACGGA TCCAGAGATT CTGGAGAGTC GGTATCCAGT TATCCTGCGC 
CGCTTTGAGC TGAGGCCAGG CTCCGGGGGC CGAGGTCGCT TCCGGGGAGG TGATGGCGTA 
GTCCGAGAGC TGGTCTTTCG GGAAGAGGCG CTGTTGTCTG TGCTCACCGA GCGCCGGGCC 
TTCCAGCCTT ACGGCCTCCA CGGGGGAGAG CCTGGTGCGC GTGGCTTAAA CCTCCTGATC 
AGAAAAGATG GGCGCACAGT GAATTTGGGC GGCAAGACAT CTGTGACCGT GTACCCCGGG 
GACGTGTTCT GCCTCCACAC GCCTGGGGGT GGGGGCTACG GAGACCCGGA GGATCCAGCG 
CCACCACCAG GCTCGCCCCC GCTATTTCCA GCCTTCCCCG AGCGCGGCAG TGTATTCGAG 
TACCGCCGCG CCCAGGAAGC CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Oplah ( XM_017594576.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450065.1 Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa30436
Clone ID Related Accession (Same CDS sequence)	XM_006241774.3 , XM_017594576.1 , XM_017594577.1 , XM_006241775.3
Accession Version	XM_017594576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	5-oxoprolinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCC CAGAAGGGCG CTTCCATTTC GCCATCGACC GCGGTGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCTGG AGGGCATGTG CGTGTCCTGA AGCTGCTCTC AGAGGACCCT 
GCCAACTATG CAGATGCACC CACAGAGGGC ATCCGCCGAA TTCTAGAGCA GGAGGAGGGT 
GTGCTGCTGC CTCGAGGCCG ACCGCTAGAC ACCAGTCGCA TTGCCAGCAT CCGCATGGGT 
ACCACGGTGG CCACCAATGC ACTGTTGGAA CGACAGGGAG AACGGGTGGC ACTGCTGGTG 
ACTCGGGGTT TCCGAGACCT GCTGCATATT GGCACTCAGG CCCGCCCGGA CCTCTTTGAC 
TTGGCTGTGC CCATGCCAGA GGTTCTGTAT GAGGAAGTGC TGGAGGTAGA TGAGCGAGTG 
GTGCTGTATC GCGGAGAACC AGGTGCCGGC TCTCCTGTCA AAGGCCGCAC AGGGGACCTG 
CTAGAGATAC AGCAGCCTGT GGACCTGGAA GCCCTGCGTG GGAAGCTGGA GGGGCTCTTG 
TCTCGGGGCA TTCACAGTCT GGCAGTGGTG CTCATGCATT CGTACACGTG GGCCCAGCAT 
GAGCAGCAGG TGGGCACGCT GGCCCGGGAG CTGGGCTTCA CGCACGTCTC CTTGTCCTCG 
GAAGTCATGC CCATGGTACG AATTGTTCCT CGGGGCCATA CAGCCTGTGC TGACGCTTAC 
CTTACTCCCA CCATCCAGCG CTATGTGCAG GGCTTCCGCC GAGGCTTCCA GGGCCAGCTA 
AAGAATGTGC AAGTTCTCTT CATGCGCTCT GATGGTGGCC TCGCACCCAT GGATGCTTTC 
AGTGGTTCCC GGGCTGTGCT CTCTGGCCCT GCTGGGGGTG TGGTTGGCTA CTCAGCTACC 
ACCTACCATC TGGAAGGCGG TCAGCCTGTC ATTGGCTTTG ACATGGGAGG CACATCCACA 
GACGTGAGCC GCTATGCTGG AGAATTTGAG CATGTCTTTG AGGCTAGCAC AGCAGGCGTT 
ACCCTTCAGG CACCCCAGTT GGACATCAAC ACAGTGGCAG CTGGCGGGGG TTCCCGCCTC 
TTCTTCAGAT CTGGCCTCTT TGTGGTTGGT CCAGAGTCAG CAGGTGCCCA CCCAGGTCCT 
GCCTGCTACC GTAAAGGGGG TCCTGTGACA GTGACAGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCAGGAG AAGACCAGCC ACTGTCTCCT 
GAGGCTTCCC GAAAGGCTCT AGAGGCTGTG GCCATGGAGG TCAACAGTTT CTTGACCAAT 
GGACCGTGCC CAGCTTCCCA ACTAAGTCTG GAAGAGGTGG CCATGGGGTT TGTGCGTGTT 
GCCAATGAAG CCATGTGCCG GCCTATCCGT GCCCTCACAC AGGCACGAGG CCATGACCCC 
TCAGCCCATG TATTGGCTTG CTTTGGAGGA GCTGGTGGGC AACACGCTTG TGCCATTGCC 
CGGGCCCTGG GGATGGATAC TGTGCACATT CACAGGCACA GCGGGCTGCT GTCAGCACTA 
GGACTGGCCT TGGCAGATGT GGTTCACGAA GCACAGGAGC CCTGTTCCCT GTCTTACACA 
CCTGAAACCT TTGCACAACT GGACCAGAGA CTGAGCCGCC TGGAGGAGCA GTGTGTGGAT 
GCCTTGCAGG TCCAGGGCTT CCCTAGGTCT CAGATCAGCA CCGAGAGCTT CCTGCATCTT 
CGCTACCAAG GCACTGACTG CGCCCTAATG GTGTCTGCCC ATCAGCATCC GGCCACAGCC 
TGCTCACCCC GTGCTGGTGA CTTTGGAGCC GCATTTGTGG AGAGGTACAT GAGAGAGTTT 
GGCTTCATTA TCCCCGAGCG GCCGGTGGTG GTAGATGATG TACGTGTGAG GGGAACTGGC 
CGTAGTGGAC TTCAGCTGGA GGACACCCCC AAAATCCAGA CTGGACCTCC CCACGTGGAA 
AAGGTGACCC AGTGCTACTT TGAAGGGGGT TATCAGGAGA CTCCCGTGTA CCTTTTAGGA 
GAACTAGGCT ACGGGCACCA GCTCCAAGGG CCCTGCCTTA TCATCGACAA CAACAGCACC 
ATCCTTGTAG AACCGGGTTG CCAAGCAGAG GTGACTGATA CAGGGGACAT CCGCATTTCT 
GTGGGAGCTG AGGGTCCTAG TATGGCAGAT ACCAGGCTTG ACCCCATCCA GCTGTCTATT 
TTCTCACACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTACA GCGCACAGCC 
ATCTCTACCA ACATCAAGGA ACGCCTCGAC TTCTCCTGTG CCCTCTTTGG GCCAGATGGG 
GGCCTCGTCT CCAATGCTCC CCACATTCCT GTGCACCTGG GTGCCATGCA AGAGACTGTA 
CAGTTCCAGA TTCAGCACTT AGGAGCCGAC CTCCATCCTG GTGATGTGTT GCTCAGCAAC 
CATCCCAGCG CAGGGGGCAG CCATCTTCCT GACCTGACTG TCATTACACC GGTGTTTTGG 
CCAGGCCAGA CGAGGCCTGT GTTCTACGTG GCTAGCCGAG GGCACCACGC AGACATTGGA 
GGAATCACAC CGGGCTCTAT GCCGCCTCAC TCCACCACGC TGCAACAGGA GGGTGCCGTT 
TTTCTGTCCT TCAAACTGGT CCAGGGAGGC GTCTTCCAGG AAGAGGCAGT GACAGAGGCC 
CTACGGGCAC CAGGCAAGAT CTCTGGCTGT AGTGGAACCA GGAACCTGCA TGACAACCTG 
TCGGATCTTC GTGCCCAGGT GGCAGCTAAC CAGAAAGGCA TCCAGCTGGT GGGAGAGCTG 
ATCGGACAGT ATGGCTTAGA TGTGGTGCAG GCCTATATGG GCCATATTCA GGCGAATGCT 
GAGCTAGCAG TGAGAGACAT GCTCCGGGCT TTTGGAACTT CCCGGCAGGC CAGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGATCACATG GATGATGGCT CTCCCATCTG TCTGCGTGTT 
CAGATCAACC TGAGTCAGGG CAGTGCGGTA TTTGACTTCA CTGGTTCCGG GTCTGAGGTG 
TTTGGCAATC TCAATGCCCC GAGAGCCATA ACACTGTCTG CTCTCATCTA TTGCTTACGC 
TGTCTAGTGG GCCGTGACAT CCCACTTAAC CAGGGTTGCC TGGCTCCTGT GCGTGTCATA 
ATTCCCAAAG GCTCCATATT GGATCCATCC CCAGAGGCAG CAGTGGTCGG CGGCAACGTG 
CTCACATCTC AGCGAGTAGT GGATGTCATT CTGGGGGCTT TTGGGGCCTG TTCAGCCTCC 
CAGGGCTGCA TGAACAATGT GACCCTGGGC AATGCCCGTA TGGGCTACTA TGAGACAGTG 
GCTGGTGGTG CCGGTGCGGG CCCTGGCTGG CATGGGCGCA GTGGTGTACA CAGTCACATG 
ACCAACACAC GCATTACGGA TCCAGAGATT CTGGAGAGTC GGTATCCAGT TATCCTGCGC 
CGCTTTGAGC TGAGGCCAGG CTCCGGGGGC CGAGGTCGCT TCCGGGGAGG TGATGGCGTA 
GTCCGAGAGC TGGTCTTTCG GGAAGAGGCG CTGTTGTCTG TGCTCACCGA GCGCCGGGCC 
TTCCAGCCTT ACGGCCTCCA CGGGGGAGAG CCTGGTGCGC GTGGCTTAAA CCTCCTGATC 
AGAAAAGATG GGCGCACAGT GAATTTGGGC GGCAAGACAT CTGTGACCGT GTACCCCGGG 
GACGTGTTCT GCCTCCACAC GCCTGGGGGT GGGGGCTACG GAGACCCGGA GGATCCAGCG 
CCACCACCAG GCTCGCCCCC GCTATTTCCA GCCTTCCCCG AGCGCGGCAG TGTATTCGAG 
TACCGCCGCG CCCAGGAAGC CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450065.1
CDS	1198..5064
Translation	MGSPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRILEQEEGVLLPRGRPLDTSRIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDLFDLAVPMPEVLYEEVLEVDERVVLYRGEPGAGSPVKGRTGDLLEIQQPVDLEALRGKLEGLLSRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSLSSEVMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAFSGSRAVLSGPAGGVVGYSATTYHLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVAMEVNSFLTNGPCPASQLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLSYTPETFAQLDQRLSRLEEQCVDALQVQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPATACSPRAGDFGAAFVERYMREFGFIIPERPVVVDDVRVRGTGRSGLQLEDTPKIQTGPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNSTILVEPGCQAEVTDTGDIRISVGAEGPSMADTRLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKISGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGLPLEVSAEDHMDDGSPICLRVQINLSQGSAVFDFTGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVIIPKGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACSASQGCMNNVTLGNARMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELVFREEALLSVLTERRAFQPYGLHGGEPGARGLNLLIRKDGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPAPPPGSPPLFPAFPERGSVFEYRRAQEAV

Target ORF information:

RefSeq Version	XM_017594576.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594576.1

ORF Insert Sequence:

ATGGGCAGCC CAGAAGGGCG CTTCCATTTC GCCATCGACC GCGGTGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCTGG AGGGCATGTG CGTGTCCTGA AGCTGCTCTC AGAGGACCCT 
GCCAACTATG CAGATGCACC CACAGAGGGC ATCCGCCGAA TTCTAGAGCA GGAGGAGGGT 
GTGCTGCTGC CTCGAGGCCG ACCGCTAGAC ACCAGTCGCA TTGCCAGCAT CCGCATGGGT 
ACCACGGTGG CCACCAATGC ACTGTTGGAA CGACAGGGAG AACGGGTGGC ACTGCTGGTG 
ACTCGGGGTT TCCGAGACCT GCTGCATATT GGCACTCAGG CCCGCCCGGA CCTCTTTGAC 
TTGGCTGTGC CCATGCCAGA GGTTCTGTAT GAGGAAGTGC TGGAGGTAGA TGAGCGAGTG 
GTGCTGTATC GCGGAGAACC AGGTGCCGGC TCTCCTGTCA AAGGCCGCAC AGGGGACCTG 
CTAGAGATAC AGCAGCCTGT GGACCTGGAA GCCCTGCGTG GGAAGCTGGA GGGGCTCTTG 
TCTCGGGGCA TTCACAGTCT GGCAGTGGTG CTCATGCATT CGTACACGTG GGCCCAGCAT 
GAGCAGCAGG TGGGCACGCT GGCCCGGGAG CTGGGCTTCA CGCACGTCTC CTTGTCCTCG 
GAAGTCATGC CCATGGTACG AATTGTTCCT CGGGGCCATA CAGCCTGTGC TGACGCTTAC 
CTTACTCCCA CCATCCAGCG CTATGTGCAG GGCTTCCGCC GAGGCTTCCA GGGCCAGCTA 
AAGAATGTGC AAGTTCTCTT CATGCGCTCT GATGGTGGCC TCGCACCCAT GGATGCTTTC 
AGTGGTTCCC GGGCTGTGCT CTCTGGCCCT GCTGGGGGTG TGGTTGGCTA CTCAGCTACC 
ACCTACCATC TGGAAGGCGG TCAGCCTGTC ATTGGCTTTG ACATGGGAGG CACATCCACA 
GACGTGAGCC GCTATGCTGG AGAATTTGAG CATGTCTTTG AGGCTAGCAC AGCAGGCGTT 
ACCCTTCAGG CACCCCAGTT GGACATCAAC ACAGTGGCAG CTGGCGGGGG TTCCCGCCTC 
TTCTTCAGAT CTGGCCTCTT TGTGGTTGGT CCAGAGTCAG CAGGTGCCCA CCCAGGTCCT 
GCCTGCTACC GTAAAGGGGG TCCTGTGACA GTGACAGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCAGGAG AAGACCAGCC ACTGTCTCCT 
GAGGCTTCCC GAAAGGCTCT AGAGGCTGTG GCCATGGAGG TCAACAGTTT CTTGACCAAT 
GGACCGTGCC CAGCTTCCCA ACTAAGTCTG GAAGAGGTGG CCATGGGGTT TGTGCGTGTT 
GCCAATGAAG CCATGTGCCG GCCTATCCGT GCCCTCACAC AGGCACGAGG CCATGACCCC 
TCAGCCCATG TATTGGCTTG CTTTGGAGGA GCTGGTGGGC AACACGCTTG TGCCATTGCC 
CGGGCCCTGG GGATGGATAC TGTGCACATT CACAGGCACA GCGGGCTGCT GTCAGCACTA 
GGACTGGCCT TGGCAGATGT GGTTCACGAA GCACAGGAGC CCTGTTCCCT GTCTTACACA 
CCTGAAACCT TTGCACAACT GGACCAGAGA CTGAGCCGCC TGGAGGAGCA GTGTGTGGAT 
GCCTTGCAGG TCCAGGGCTT CCCTAGGTCT CAGATCAGCA CCGAGAGCTT CCTGCATCTT 
CGCTACCAAG GCACTGACTG CGCCCTAATG GTGTCTGCCC ATCAGCATCC GGCCACAGCC 
TGCTCACCCC GTGCTGGTGA CTTTGGAGCC GCATTTGTGG AGAGGTACAT GAGAGAGTTT 
GGCTTCATTA TCCCCGAGCG GCCGGTGGTG GTAGATGATG TACGTGTGAG GGGAACTGGC 
CGTAGTGGAC TTCAGCTGGA GGACACCCCC AAAATCCAGA CTGGACCTCC CCACGTGGAA 
AAGGTGACCC AGTGCTACTT TGAAGGGGGT TATCAGGAGA CTCCCGTGTA CCTTTTAGGA 
GAACTAGGCT ACGGGCACCA GCTCCAAGGG CCCTGCCTTA TCATCGACAA CAACAGCACC 
ATCCTTGTAG AACCGGGTTG CCAAGCAGAG GTGACTGATA CAGGGGACAT CCGCATTTCT 
GTGGGAGCTG AGGGTCCTAG TATGGCAGAT ACCAGGCTTG ACCCCATCCA GCTGTCTATT 
TTCTCACACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTACA GCGCACAGCC 
ATCTCTACCA ACATCAAGGA ACGCCTCGAC TTCTCCTGTG CCCTCTTTGG GCCAGATGGG 
GGCCTCGTCT CCAATGCTCC CCACATTCCT GTGCACCTGG GTGCCATGCA AGAGACTGTA 
CAGTTCCAGA TTCAGCACTT AGGAGCCGAC CTCCATCCTG GTGATGTGTT GCTCAGCAAC 
CATCCCAGCG CAGGGGGCAG CCATCTTCCT GACCTGACTG TCATTACACC GGTGTTTTGG 
CCAGGCCAGA CGAGGCCTGT GTTCTACGTG GCTAGCCGAG GGCACCACGC AGACATTGGA 
GGAATCACAC CGGGCTCTAT GCCGCCTCAC TCCACCACGC TGCAACAGGA GGGTGCCGTT 
TTTCTGTCCT TCAAACTGGT CCAGGGAGGC GTCTTCCAGG AAGAGGCAGT GACAGAGGCC 
CTACGGGCAC CAGGCAAGAT CTCTGGCTGT AGTGGAACCA GGAACCTGCA TGACAACCTG 
TCGGATCTTC GTGCCCAGGT GGCAGCTAAC CAGAAAGGCA TCCAGCTGGT GGGAGAGCTG 
ATCGGACAGT ATGGCTTAGA TGTGGTGCAG GCCTATATGG GCCATATTCA GGCGAATGCT 
GAGCTAGCAG TGAGAGACAT GCTCCGGGCT TTTGGAACTT CCCGGCAGGC CAGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGATCACATG GATGATGGCT CTCCCATCTG TCTGCGTGTT 
CAGATCAACC TGAGTCAGGG CAGTGCGGTA TTTGACTTCA CTGGTTCCGG GTCTGAGGTG 
TTTGGCAATC TCAATGCCCC GAGAGCCATA ACACTGTCTG CTCTCATCTA TTGCTTACGC 
TGTCTAGTGG GCCGTGACAT CCCACTTAAC CAGGGTTGCC TGGCTCCTGT GCGTGTCATA 
ATTCCCAAAG GCTCCATATT GGATCCATCC CCAGAGGCAG CAGTGGTCGG CGGCAACGTG 
CTCACATCTC AGCGAGTAGT GGATGTCATT CTGGGGGCTT TTGGGGCCTG TTCAGCCTCC 
CAGGGCTGCA TGAACAATGT GACCCTGGGC AATGCCCGTA TGGGCTACTA TGAGACAGTG 
GCTGGTGGTG CCGGTGCGGG CCCTGGCTGG CATGGGCGCA GTGGTGTACA CAGTCACATG 
ACCAACACAC GCATTACGGA TCCAGAGATT CTGGAGAGTC GGTATCCAGT TATCCTGCGC 
CGCTTTGAGC TGAGGCCAGG CTCCGGGGGC CGAGGTCGCT TCCGGGGAGG TGATGGCGTA 
GTCCGAGAGC TGGTCTTTCG GGAAGAGGCG CTGTTGTCTG TGCTCACCGA GCGCCGGGCC 
TTCCAGCCTT ACGGCCTCCA CGGGGGAGAG CCTGGTGCGC GTGGCTTAAA CCTCCTGATC 
AGAAAAGATG GGCGCACAGT GAATTTGGGC GGCAAGACAT CTGTGACCGT GTACCCCGGG 
GACGTGTTCT GCCTCCACAC GCCTGGGGGT GGGGGCTACG GAGACCCGGA GGATCCAGCG 
CCACCACCAG GCTCGCCCCC GCTATTTCCA GCCTTCCCCG AGCGCGGCAG TGTATTCGAG 
TACCGCCGCG CCCAGGAAGC CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Oplah ( XM_017594577.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450066.1 Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X7, mRNA.

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CloneID	ORa30436
Clone ID Related Accession (Same CDS sequence)	XM_006241774.3 , XM_017594576.1 , XM_017594577.1 , XM_006241775.3
Accession Version	XM_017594577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	5-oxoprolinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCC CAGAAGGGCG CTTCCATTTC GCCATCGACC GCGGTGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCTGG AGGGCATGTG CGTGTCCTGA AGCTGCTCTC AGAGGACCCT 
GCCAACTATG CAGATGCACC CACAGAGGGC ATCCGCCGAA TTCTAGAGCA GGAGGAGGGT 
GTGCTGCTGC CTCGAGGCCG ACCGCTAGAC ACCAGTCGCA TTGCCAGCAT CCGCATGGGT 
ACCACGGTGG CCACCAATGC ACTGTTGGAA CGACAGGGAG AACGGGTGGC ACTGCTGGTG 
ACTCGGGGTT TCCGAGACCT GCTGCATATT GGCACTCAGG CCCGCCCGGA CCTCTTTGAC 
TTGGCTGTGC CCATGCCAGA GGTTCTGTAT GAGGAAGTGC TGGAGGTAGA TGAGCGAGTG 
GTGCTGTATC GCGGAGAACC AGGTGCCGGC TCTCCTGTCA AAGGCCGCAC AGGGGACCTG 
CTAGAGATAC AGCAGCCTGT GGACCTGGAA GCCCTGCGTG GGAAGCTGGA GGGGCTCTTG 
TCTCGGGGCA TTCACAGTCT GGCAGTGGTG CTCATGCATT CGTACACGTG GGCCCAGCAT 
GAGCAGCAGG TGGGCACGCT GGCCCGGGAG CTGGGCTTCA CGCACGTCTC CTTGTCCTCG 
GAAGTCATGC CCATGGTACG AATTGTTCCT CGGGGCCATA CAGCCTGTGC TGACGCTTAC 
CTTACTCCCA CCATCCAGCG CTATGTGCAG GGCTTCCGCC GAGGCTTCCA GGGCCAGCTA 
AAGAATGTGC AAGTTCTCTT CATGCGCTCT GATGGTGGCC TCGCACCCAT GGATGCTTTC 
AGTGGTTCCC GGGCTGTGCT CTCTGGCCCT GCTGGGGGTG TGGTTGGCTA CTCAGCTACC 
ACCTACCATC TGGAAGGCGG TCAGCCTGTC ATTGGCTTTG ACATGGGAGG CACATCCACA 
GACGTGAGCC GCTATGCTGG AGAATTTGAG CATGTCTTTG AGGCTAGCAC AGCAGGCGTT 
ACCCTTCAGG CACCCCAGTT GGACATCAAC ACAGTGGCAG CTGGCGGGGG TTCCCGCCTC 
TTCTTCAGAT CTGGCCTCTT TGTGGTTGGT CCAGAGTCAG CAGGTGCCCA CCCAGGTCCT 
GCCTGCTACC GTAAAGGGGG TCCTGTGACA GTGACAGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCAGGAG AAGACCAGCC ACTGTCTCCT 
GAGGCTTCCC GAAAGGCTCT AGAGGCTGTG GCCATGGAGG TCAACAGTTT CTTGACCAAT 
GGACCGTGCC CAGCTTCCCA ACTAAGTCTG GAAGAGGTGG CCATGGGGTT TGTGCGTGTT 
GCCAATGAAG CCATGTGCCG GCCTATCCGT GCCCTCACAC AGGCACGAGG CCATGACCCC 
TCAGCCCATG TATTGGCTTG CTTTGGAGGA GCTGGTGGGC AACACGCTTG TGCCATTGCC 
CGGGCCCTGG GGATGGATAC TGTGCACATT CACAGGCACA GCGGGCTGCT GTCAGCACTA 
GGACTGGCCT TGGCAGATGT GGTTCACGAA GCACAGGAGC CCTGTTCCCT GTCTTACACA 
CCTGAAACCT TTGCACAACT GGACCAGAGA CTGAGCCGCC TGGAGGAGCA GTGTGTGGAT 
GCCTTGCAGG TCCAGGGCTT CCCTAGGTCT CAGATCAGCA CCGAGAGCTT CCTGCATCTT 
CGCTACCAAG GCACTGACTG CGCCCTAATG GTGTCTGCCC ATCAGCATCC GGCCACAGCC 
TGCTCACCCC GTGCTGGTGA CTTTGGAGCC GCATTTGTGG AGAGGTACAT GAGAGAGTTT 
GGCTTCATTA TCCCCGAGCG GCCGGTGGTG GTAGATGATG TACGTGTGAG GGGAACTGGC 
CGTAGTGGAC TTCAGCTGGA GGACACCCCC AAAATCCAGA CTGGACCTCC CCACGTGGAA 
AAGGTGACCC AGTGCTACTT TGAAGGGGGT TATCAGGAGA CTCCCGTGTA CCTTTTAGGA 
GAACTAGGCT ACGGGCACCA GCTCCAAGGG CCCTGCCTTA TCATCGACAA CAACAGCACC 
ATCCTTGTAG AACCGGGTTG CCAAGCAGAG GTGACTGATA CAGGGGACAT CCGCATTTCT 
GTGGGAGCTG AGGGTCCTAG TATGGCAGAT ACCAGGCTTG ACCCCATCCA GCTGTCTATT 
TTCTCACACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTACA GCGCACAGCC 
ATCTCTACCA ACATCAAGGA ACGCCTCGAC TTCTCCTGTG CCCTCTTTGG GCCAGATGGG 
GGCCTCGTCT CCAATGCTCC CCACATTCCT GTGCACCTGG GTGCCATGCA AGAGACTGTA 
CAGTTCCAGA TTCAGCACTT AGGAGCCGAC CTCCATCCTG GTGATGTGTT GCTCAGCAAC 
CATCCCAGCG CAGGGGGCAG CCATCTTCCT GACCTGACTG TCATTACACC GGTGTTTTGG 
CCAGGCCAGA CGAGGCCTGT GTTCTACGTG GCTAGCCGAG GGCACCACGC AGACATTGGA 
GGAATCACAC CGGGCTCTAT GCCGCCTCAC TCCACCACGC TGCAACAGGA GGGTGCCGTT 
TTTCTGTCCT TCAAACTGGT CCAGGGAGGC GTCTTCCAGG AAGAGGCAGT GACAGAGGCC 
CTACGGGCAC CAGGCAAGAT CTCTGGCTGT AGTGGAACCA GGAACCTGCA TGACAACCTG 
TCGGATCTTC GTGCCCAGGT GGCAGCTAAC CAGAAAGGCA TCCAGCTGGT GGGAGAGCTG 
ATCGGACAGT ATGGCTTAGA TGTGGTGCAG GCCTATATGG GCCATATTCA GGCGAATGCT 
GAGCTAGCAG TGAGAGACAT GCTCCGGGCT TTTGGAACTT CCCGGCAGGC CAGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGATCACATG GATGATGGCT CTCCCATCTG TCTGCGTGTT 
CAGATCAACC TGAGTCAGGG CAGTGCGGTA TTTGACTTCA CTGGTTCCGG GTCTGAGGTG 
TTTGGCAATC TCAATGCCCC GAGAGCCATA ACACTGTCTG CTCTCATCTA TTGCTTACGC 
TGTCTAGTGG GCCGTGACAT CCCACTTAAC CAGGGTTGCC TGGCTCCTGT GCGTGTCATA 
ATTCCCAAAG GCTCCATATT GGATCCATCC CCAGAGGCAG CAGTGGTCGG CGGCAACGTG 
CTCACATCTC AGCGAGTAGT GGATGTCATT CTGGGGGCTT TTGGGGCCTG TTCAGCCTCC 
CAGGGCTGCA TGAACAATGT GACCCTGGGC AATGCCCGTA TGGGCTACTA TGAGACAGTG 
GCTGGTGGTG CCGGTGCGGG CCCTGGCTGG CATGGGCGCA GTGGTGTACA CAGTCACATG 
ACCAACACAC GCATTACGGA TCCAGAGATT CTGGAGAGTC GGTATCCAGT TATCCTGCGC 
CGCTTTGAGC TGAGGCCAGG CTCCGGGGGC CGAGGTCGCT TCCGGGGAGG TGATGGCGTA 
GTCCGAGAGC TGGTCTTTCG GGAAGAGGCG CTGTTGTCTG TGCTCACCGA GCGCCGGGCC 
TTCCAGCCTT ACGGCCTCCA CGGGGGAGAG CCTGGTGCGC GTGGCTTAAA CCTCCTGATC 
AGAAAAGATG GGCGCACAGT GAATTTGGGC GGCAAGACAT CTGTGACCGT GTACCCCGGG 
GACGTGTTCT GCCTCCACAC GCCTGGGGGT GGGGGCTACG GAGACCCGGA GGATCCAGCG 
CCACCACCAG GCTCGCCCCC GCTATTTCCA GCCTTCCCCG AGCGCGGCAG TGTATTCGAG 
TACCGCCGCG CCCAGGAAGC CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450066.1
CDS	161..4027
Translation	MGSPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRILEQEEGVLLPRGRPLDTSRIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDLFDLAVPMPEVLYEEVLEVDERVVLYRGEPGAGSPVKGRTGDLLEIQQPVDLEALRGKLEGLLSRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSLSSEVMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAFSGSRAVLSGPAGGVVGYSATTYHLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVAMEVNSFLTNGPCPASQLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLSYTPETFAQLDQRLSRLEEQCVDALQVQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPATACSPRAGDFGAAFVERYMREFGFIIPERPVVVDDVRVRGTGRSGLQLEDTPKIQTGPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNSTILVEPGCQAEVTDTGDIRISVGAEGPSMADTRLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKISGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGLPLEVSAEDHMDDGSPICLRVQINLSQGSAVFDFTGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVIIPKGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACSASQGCMNNVTLGNARMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELVFREEALLSVLTERRAFQPYGLHGGEPGARGLNLLIRKDGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPAPPPGSPPLFPAFPERGSVFEYRRAQEAV

Target ORF information:

RefSeq Version	XM_017594577.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594577.1

ORF Insert Sequence:

ATGGGCAGCC CAGAAGGGCG CTTCCATTTC GCCATCGACC GCGGTGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCTGG AGGGCATGTG CGTGTCCTGA AGCTGCTCTC AGAGGACCCT 
GCCAACTATG CAGATGCACC CACAGAGGGC ATCCGCCGAA TTCTAGAGCA GGAGGAGGGT 
GTGCTGCTGC CTCGAGGCCG ACCGCTAGAC ACCAGTCGCA TTGCCAGCAT CCGCATGGGT 
ACCACGGTGG CCACCAATGC ACTGTTGGAA CGACAGGGAG AACGGGTGGC ACTGCTGGTG 
ACTCGGGGTT TCCGAGACCT GCTGCATATT GGCACTCAGG CCCGCCCGGA CCTCTTTGAC 
TTGGCTGTGC CCATGCCAGA GGTTCTGTAT GAGGAAGTGC TGGAGGTAGA TGAGCGAGTG 
GTGCTGTATC GCGGAGAACC AGGTGCCGGC TCTCCTGTCA AAGGCCGCAC AGGGGACCTG 
CTAGAGATAC AGCAGCCTGT GGACCTGGAA GCCCTGCGTG GGAAGCTGGA GGGGCTCTTG 
TCTCGGGGCA TTCACAGTCT GGCAGTGGTG CTCATGCATT CGTACACGTG GGCCCAGCAT 
GAGCAGCAGG TGGGCACGCT GGCCCGGGAG CTGGGCTTCA CGCACGTCTC CTTGTCCTCG 
GAAGTCATGC CCATGGTACG AATTGTTCCT CGGGGCCATA CAGCCTGTGC TGACGCTTAC 
CTTACTCCCA CCATCCAGCG CTATGTGCAG GGCTTCCGCC GAGGCTTCCA GGGCCAGCTA 
AAGAATGTGC AAGTTCTCTT CATGCGCTCT GATGGTGGCC TCGCACCCAT GGATGCTTTC 
AGTGGTTCCC GGGCTGTGCT CTCTGGCCCT GCTGGGGGTG TGGTTGGCTA CTCAGCTACC 
ACCTACCATC TGGAAGGCGG TCAGCCTGTC ATTGGCTTTG ACATGGGAGG CACATCCACA 
GACGTGAGCC GCTATGCTGG AGAATTTGAG CATGTCTTTG AGGCTAGCAC AGCAGGCGTT 
ACCCTTCAGG CACCCCAGTT GGACATCAAC ACAGTGGCAG CTGGCGGGGG TTCCCGCCTC 
TTCTTCAGAT CTGGCCTCTT TGTGGTTGGT CCAGAGTCAG CAGGTGCCCA CCCAGGTCCT 
GCCTGCTACC GTAAAGGGGG TCCTGTGACA GTGACAGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCAGGAG AAGACCAGCC ACTGTCTCCT 
GAGGCTTCCC GAAAGGCTCT AGAGGCTGTG GCCATGGAGG TCAACAGTTT CTTGACCAAT 
GGACCGTGCC CAGCTTCCCA ACTAAGTCTG GAAGAGGTGG CCATGGGGTT TGTGCGTGTT 
GCCAATGAAG CCATGTGCCG GCCTATCCGT GCCCTCACAC AGGCACGAGG CCATGACCCC 
TCAGCCCATG TATTGGCTTG CTTTGGAGGA GCTGGTGGGC AACACGCTTG TGCCATTGCC 
CGGGCCCTGG GGATGGATAC TGTGCACATT CACAGGCACA GCGGGCTGCT GTCAGCACTA 
GGACTGGCCT TGGCAGATGT GGTTCACGAA GCACAGGAGC CCTGTTCCCT GTCTTACACA 
CCTGAAACCT TTGCACAACT GGACCAGAGA CTGAGCCGCC TGGAGGAGCA GTGTGTGGAT 
GCCTTGCAGG TCCAGGGCTT CCCTAGGTCT CAGATCAGCA CCGAGAGCTT CCTGCATCTT 
CGCTACCAAG GCACTGACTG CGCCCTAATG GTGTCTGCCC ATCAGCATCC GGCCACAGCC 
TGCTCACCCC GTGCTGGTGA CTTTGGAGCC GCATTTGTGG AGAGGTACAT GAGAGAGTTT 
GGCTTCATTA TCCCCGAGCG GCCGGTGGTG GTAGATGATG TACGTGTGAG GGGAACTGGC 
CGTAGTGGAC TTCAGCTGGA GGACACCCCC AAAATCCAGA CTGGACCTCC CCACGTGGAA 
AAGGTGACCC AGTGCTACTT TGAAGGGGGT TATCAGGAGA CTCCCGTGTA CCTTTTAGGA 
GAACTAGGCT ACGGGCACCA GCTCCAAGGG CCCTGCCTTA TCATCGACAA CAACAGCACC 
ATCCTTGTAG AACCGGGTTG CCAAGCAGAG GTGACTGATA CAGGGGACAT CCGCATTTCT 
GTGGGAGCTG AGGGTCCTAG TATGGCAGAT ACCAGGCTTG ACCCCATCCA GCTGTCTATT 
TTCTCACACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTACA GCGCACAGCC 
ATCTCTACCA ACATCAAGGA ACGCCTCGAC TTCTCCTGTG CCCTCTTTGG GCCAGATGGG 
GGCCTCGTCT CCAATGCTCC CCACATTCCT GTGCACCTGG GTGCCATGCA AGAGACTGTA 
CAGTTCCAGA TTCAGCACTT AGGAGCCGAC CTCCATCCTG GTGATGTGTT GCTCAGCAAC 
CATCCCAGCG CAGGGGGCAG CCATCTTCCT GACCTGACTG TCATTACACC GGTGTTTTGG 
CCAGGCCAGA CGAGGCCTGT GTTCTACGTG GCTAGCCGAG GGCACCACGC AGACATTGGA 
GGAATCACAC CGGGCTCTAT GCCGCCTCAC TCCACCACGC TGCAACAGGA GGGTGCCGTT 
TTTCTGTCCT TCAAACTGGT CCAGGGAGGC GTCTTCCAGG AAGAGGCAGT GACAGAGGCC 
CTACGGGCAC CAGGCAAGAT CTCTGGCTGT AGTGGAACCA GGAACCTGCA TGACAACCTG 
TCGGATCTTC GTGCCCAGGT GGCAGCTAAC CAGAAAGGCA TCCAGCTGGT GGGAGAGCTG 
ATCGGACAGT ATGGCTTAGA TGTGGTGCAG GCCTATATGG GCCATATTCA GGCGAATGCT 
GAGCTAGCAG TGAGAGACAT GCTCCGGGCT TTTGGAACTT CCCGGCAGGC CAGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGATCACATG GATGATGGCT CTCCCATCTG TCTGCGTGTT 
CAGATCAACC TGAGTCAGGG CAGTGCGGTA TTTGACTTCA CTGGTTCCGG GTCTGAGGTG 
TTTGGCAATC TCAATGCCCC GAGAGCCATA ACACTGTCTG CTCTCATCTA TTGCTTACGC 
TGTCTAGTGG GCCGTGACAT CCCACTTAAC CAGGGTTGCC TGGCTCCTGT GCGTGTCATA 
ATTCCCAAAG GCTCCATATT GGATCCATCC CCAGAGGCAG CAGTGGTCGG CGGCAACGTG 
CTCACATCTC AGCGAGTAGT GGATGTCATT CTGGGGGCTT TTGGGGCCTG TTCAGCCTCC 
CAGGGCTGCA TGAACAATGT GACCCTGGGC AATGCCCGTA TGGGCTACTA TGAGACAGTG 
GCTGGTGGTG CCGGTGCGGG CCCTGGCTGG CATGGGCGCA GTGGTGTACA CAGTCACATG 
ACCAACACAC GCATTACGGA TCCAGAGATT CTGGAGAGTC GGTATCCAGT TATCCTGCGC 
CGCTTTGAGC TGAGGCCAGG CTCCGGGGGC CGAGGTCGCT TCCGGGGAGG TGATGGCGTA 
GTCCGAGAGC TGGTCTTTCG GGAAGAGGCG CTGTTGTCTG TGCTCACCGA GCGCCGGGCC 
TTCCAGCCTT ACGGCCTCCA CGGGGGAGAG CCTGGTGCGC GTGGCTTAAA CCTCCTGATC 
AGAAAAGATG GGCGCACAGT GAATTTGGGC GGCAAGACAT CTGTGACCGT GTACCCCGGG 
GACGTGTTCT GCCTCCACAC GCCTGGGGGT GGGGGCTACG GAGACCCGGA GGATCCAGCG 
CCACCACCAG GCTCGCCCCC GCTATTTCCA GCCTTCCCCG AGCGCGGCAG TGTATTCGAG 
TACCGCCGCG CCCAGGAAGC CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Oplah ( XM_006241775.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241837.1 Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X6, mRNA.

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CloneID	ORa30436
Clone ID Related Accession (Same CDS sequence)	XM_006241774.3 , XM_017594576.1 , XM_017594577.1 , XM_006241775.3
Accession Version	XM_006241775.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	5-oxoprolinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241775.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCC CAGAAGGGCG CTTCCATTTC GCCATCGACC GCGGTGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCTGG AGGGCATGTG CGTGTCCTGA AGCTGCTCTC AGAGGACCCT 
GCCAACTATG CAGATGCACC CACAGAGGGC ATCCGCCGAA TTCTAGAGCA GGAGGAGGGT 
GTGCTGCTGC CTCGAGGCCG ACCGCTAGAC ACCAGTCGCA TTGCCAGCAT CCGCATGGGT 
ACCACGGTGG CCACCAATGC ACTGTTGGAA CGACAGGGAG AACGGGTGGC ACTGCTGGTG 
ACTCGGGGTT TCCGAGACCT GCTGCATATT GGCACTCAGG CCCGCCCGGA CCTCTTTGAC 
TTGGCTGTGC CCATGCCAGA GGTTCTGTAT GAGGAAGTGC TGGAGGTAGA TGAGCGAGTG 
GTGCTGTATC GCGGAGAACC AGGTGCCGGC TCTCCTGTCA AAGGCCGCAC AGGGGACCTG 
CTAGAGATAC AGCAGCCTGT GGACCTGGAA GCCCTGCGTG GGAAGCTGGA GGGGCTCTTG 
TCTCGGGGCA TTCACAGTCT GGCAGTGGTG CTCATGCATT CGTACACGTG GGCCCAGCAT 
GAGCAGCAGG TGGGCACGCT GGCCCGGGAG CTGGGCTTCA CGCACGTCTC CTTGTCCTCG 
GAAGTCATGC CCATGGTACG AATTGTTCCT CGGGGCCATA CAGCCTGTGC TGACGCTTAC 
CTTACTCCCA CCATCCAGCG CTATGTGCAG GGCTTCCGCC GAGGCTTCCA GGGCCAGCTA 
AAGAATGTGC AAGTTCTCTT CATGCGCTCT GATGGTGGCC TCGCACCCAT GGATGCTTTC 
AGTGGTTCCC GGGCTGTGCT CTCTGGCCCT GCTGGGGGTG TGGTTGGCTA CTCAGCTACC 
ACCTACCATC TGGAAGGCGG TCAGCCTGTC ATTGGCTTTG ACATGGGAGG CACATCCACA 
GACGTGAGCC GCTATGCTGG AGAATTTGAG CATGTCTTTG AGGCTAGCAC AGCAGGCGTT 
ACCCTTCAGG CACCCCAGTT GGACATCAAC ACAGTGGCAG CTGGCGGGGG TTCCCGCCTC 
TTCTTCAGAT CTGGCCTCTT TGTGGTTGGT CCAGAGTCAG CAGGTGCCCA CCCAGGTCCT 
GCCTGCTACC GTAAAGGGGG TCCTGTGACA GTGACAGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCAGGAG AAGACCAGCC ACTGTCTCCT 
GAGGCTTCCC GAAAGGCTCT AGAGGCTGTG GCCATGGAGG TCAACAGTTT CTTGACCAAT 
GGACCGTGCC CAGCTTCCCA ACTAAGTCTG GAAGAGGTGG CCATGGGGTT TGTGCGTGTT 
GCCAATGAAG CCATGTGCCG GCCTATCCGT GCCCTCACAC AGGCACGAGG CCATGACCCC 
TCAGCCCATG TATTGGCTTG CTTTGGAGGA GCTGGTGGGC AACACGCTTG TGCCATTGCC 
CGGGCCCTGG GGATGGATAC TGTGCACATT CACAGGCACA GCGGGCTGCT GTCAGCACTA 
GGACTGGCCT TGGCAGATGT GGTTCACGAA GCACAGGAGC CCTGTTCCCT GTCTTACACA 
CCTGAAACCT TTGCACAACT GGACCAGAGA CTGAGCCGCC TGGAGGAGCA GTGTGTGGAT 
GCCTTGCAGG TCCAGGGCTT CCCTAGGTCT CAGATCAGCA CCGAGAGCTT CCTGCATCTT 
CGCTACCAAG GCACTGACTG CGCCCTAATG GTGTCTGCCC ATCAGCATCC GGCCACAGCC 
TGCTCACCCC GTGCTGGTGA CTTTGGAGCC GCATTTGTGG AGAGGTACAT GAGAGAGTTT 
GGCTTCATTA TCCCCGAGCG GCCGGTGGTG GTAGATGATG TACGTGTGAG GGGAACTGGC 
CGTAGTGGAC TTCAGCTGGA GGACACCCCC AAAATCCAGA CTGGACCTCC CCACGTGGAA 
AAGGTGACCC AGTGCTACTT TGAAGGGGGT TATCAGGAGA CTCCCGTGTA CCTTTTAGGA 
GAACTAGGCT ACGGGCACCA GCTCCAAGGG CCCTGCCTTA TCATCGACAA CAACAGCACC 
ATCCTTGTAG AACCGGGTTG CCAAGCAGAG GTGACTGATA CAGGGGACAT CCGCATTTCT 
GTGGGAGCTG AGGGTCCTAG TATGGCAGAT ACCAGGCTTG ACCCCATCCA GCTGTCTATT 
TTCTCACACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTACA GCGCACAGCC 
ATCTCTACCA ACATCAAGGA ACGCCTCGAC TTCTCCTGTG CCCTCTTTGG GCCAGATGGG 
GGCCTCGTCT CCAATGCTCC CCACATTCCT GTGCACCTGG GTGCCATGCA AGAGACTGTA 
CAGTTCCAGA TTCAGCACTT AGGAGCCGAC CTCCATCCTG GTGATGTGTT GCTCAGCAAC 
CATCCCAGCG CAGGGGGCAG CCATCTTCCT GACCTGACTG TCATTACACC GGTGTTTTGG 
CCAGGCCAGA CGAGGCCTGT GTTCTACGTG GCTAGCCGAG GGCACCACGC AGACATTGGA 
GGAATCACAC CGGGCTCTAT GCCGCCTCAC TCCACCACGC TGCAACAGGA GGGTGCCGTT 
TTTCTGTCCT TCAAACTGGT CCAGGGAGGC GTCTTCCAGG AAGAGGCAGT GACAGAGGCC 
CTACGGGCAC CAGGCAAGAT CTCTGGCTGT AGTGGAACCA GGAACCTGCA TGACAACCTG 
TCGGATCTTC GTGCCCAGGT GGCAGCTAAC CAGAAAGGCA TCCAGCTGGT GGGAGAGCTG 
ATCGGACAGT ATGGCTTAGA TGTGGTGCAG GCCTATATGG GCCATATTCA GGCGAATGCT 
GAGCTAGCAG TGAGAGACAT GCTCCGGGCT TTTGGAACTT CCCGGCAGGC CAGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGATCACATG GATGATGGCT CTCCCATCTG TCTGCGTGTT 
CAGATCAACC TGAGTCAGGG CAGTGCGGTA TTTGACTTCA CTGGTTCCGG GTCTGAGGTG 
TTTGGCAATC TCAATGCCCC GAGAGCCATA ACACTGTCTG CTCTCATCTA TTGCTTACGC 
TGTCTAGTGG GCCGTGACAT CCCACTTAAC CAGGGTTGCC TGGCTCCTGT GCGTGTCATA 
ATTCCCAAAG GCTCCATATT GGATCCATCC CCAGAGGCAG CAGTGGTCGG CGGCAACGTG 
CTCACATCTC AGCGAGTAGT GGATGTCATT CTGGGGGCTT TTGGGGCCTG TTCAGCCTCC 
CAGGGCTGCA TGAACAATGT GACCCTGGGC AATGCCCGTA TGGGCTACTA TGAGACAGTG 
GCTGGTGGTG CCGGTGCGGG CCCTGGCTGG CATGGGCGCA GTGGTGTACA CAGTCACATG 
ACCAACACAC GCATTACGGA TCCAGAGATT CTGGAGAGTC GGTATCCAGT TATCCTGCGC 
CGCTTTGAGC TGAGGCCAGG CTCCGGGGGC CGAGGTCGCT TCCGGGGAGG TGATGGCGTA 
GTCCGAGAGC TGGTCTTTCG GGAAGAGGCG CTGTTGTCTG TGCTCACCGA GCGCCGGGCC 
TTCCAGCCTT ACGGCCTCCA CGGGGGAGAG CCTGGTGCGC GTGGCTTAAA CCTCCTGATC 
AGAAAAGATG GGCGCACAGT GAATTTGGGC GGCAAGACAT CTGTGACCGT GTACCCCGGG 
GACGTGTTCT GCCTCCACAC GCCTGGGGGT GGGGGCTACG GAGACCCGGA GGATCCAGCG 
CCACCACCAG GCTCGCCCCC GCTATTTCCA GCCTTCCCCG AGCGCGGCAG TGTATTCGAG 
TACCGCCGCG CCCAGGAAGC CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241837.1
CDS	307..4173
Translation	MGSPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRILEQEEGVLLPRGRPLDTSRIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDLFDLAVPMPEVLYEEVLEVDERVVLYRGEPGAGSPVKGRTGDLLEIQQPVDLEALRGKLEGLLSRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSLSSEVMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAFSGSRAVLSGPAGGVVGYSATTYHLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVAMEVNSFLTNGPCPASQLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLSYTPETFAQLDQRLSRLEEQCVDALQVQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPATACSPRAGDFGAAFVERYMREFGFIIPERPVVVDDVRVRGTGRSGLQLEDTPKIQTGPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNSTILVEPGCQAEVTDTGDIRISVGAEGPSMADTRLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKISGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGLPLEVSAEDHMDDGSPICLRVQINLSQGSAVFDFTGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVIIPKGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACSASQGCMNNVTLGNARMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELVFREEALLSVLTERRAFQPYGLHGGEPGARGLNLLIRKDGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPAPPPGSPPLFPAFPERGSVFEYRRAQEAV

Target ORF information:

RefSeq Version	XM_006241775.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241775.3

ORF Insert Sequence:

ATGGGCAGCC CAGAAGGGCG CTTCCATTTC GCCATCGACC GCGGTGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCTGG AGGGCATGTG CGTGTCCTGA AGCTGCTCTC AGAGGACCCT 
GCCAACTATG CAGATGCACC CACAGAGGGC ATCCGCCGAA TTCTAGAGCA GGAGGAGGGT 
GTGCTGCTGC CTCGAGGCCG ACCGCTAGAC ACCAGTCGCA TTGCCAGCAT CCGCATGGGT 
ACCACGGTGG CCACCAATGC ACTGTTGGAA CGACAGGGAG AACGGGTGGC ACTGCTGGTG 
ACTCGGGGTT TCCGAGACCT GCTGCATATT GGCACTCAGG CCCGCCCGGA CCTCTTTGAC 
TTGGCTGTGC CCATGCCAGA GGTTCTGTAT GAGGAAGTGC TGGAGGTAGA TGAGCGAGTG 
GTGCTGTATC GCGGAGAACC AGGTGCCGGC TCTCCTGTCA AAGGCCGCAC AGGGGACCTG 
CTAGAGATAC AGCAGCCTGT GGACCTGGAA GCCCTGCGTG GGAAGCTGGA GGGGCTCTTG 
TCTCGGGGCA TTCACAGTCT GGCAGTGGTG CTCATGCATT CGTACACGTG GGCCCAGCAT 
GAGCAGCAGG TGGGCACGCT GGCCCGGGAG CTGGGCTTCA CGCACGTCTC CTTGTCCTCG 
GAAGTCATGC CCATGGTACG AATTGTTCCT CGGGGCCATA CAGCCTGTGC TGACGCTTAC 
CTTACTCCCA CCATCCAGCG CTATGTGCAG GGCTTCCGCC GAGGCTTCCA GGGCCAGCTA 
AAGAATGTGC AAGTTCTCTT CATGCGCTCT GATGGTGGCC TCGCACCCAT GGATGCTTTC 
AGTGGTTCCC GGGCTGTGCT CTCTGGCCCT GCTGGGGGTG TGGTTGGCTA CTCAGCTACC 
ACCTACCATC TGGAAGGCGG TCAGCCTGTC ATTGGCTTTG ACATGGGAGG CACATCCACA 
GACGTGAGCC GCTATGCTGG AGAATTTGAG CATGTCTTTG AGGCTAGCAC AGCAGGCGTT 
ACCCTTCAGG CACCCCAGTT GGACATCAAC ACAGTGGCAG CTGGCGGGGG TTCCCGCCTC 
TTCTTCAGAT CTGGCCTCTT TGTGGTTGGT CCAGAGTCAG CAGGTGCCCA CCCAGGTCCT 
GCCTGCTACC GTAAAGGGGG TCCTGTGACA GTGACAGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCAGGAG AAGACCAGCC ACTGTCTCCT 
GAGGCTTCCC GAAAGGCTCT AGAGGCTGTG GCCATGGAGG TCAACAGTTT CTTGACCAAT 
GGACCGTGCC CAGCTTCCCA ACTAAGTCTG GAAGAGGTGG CCATGGGGTT TGTGCGTGTT 
GCCAATGAAG CCATGTGCCG GCCTATCCGT GCCCTCACAC AGGCACGAGG CCATGACCCC 
TCAGCCCATG TATTGGCTTG CTTTGGAGGA GCTGGTGGGC AACACGCTTG TGCCATTGCC 
CGGGCCCTGG GGATGGATAC TGTGCACATT CACAGGCACA GCGGGCTGCT GTCAGCACTA 
GGACTGGCCT TGGCAGATGT GGTTCACGAA GCACAGGAGC CCTGTTCCCT GTCTTACACA 
CCTGAAACCT TTGCACAACT GGACCAGAGA CTGAGCCGCC TGGAGGAGCA GTGTGTGGAT 
GCCTTGCAGG TCCAGGGCTT CCCTAGGTCT CAGATCAGCA CCGAGAGCTT CCTGCATCTT 
CGCTACCAAG GCACTGACTG CGCCCTAATG GTGTCTGCCC ATCAGCATCC GGCCACAGCC 
TGCTCACCCC GTGCTGGTGA CTTTGGAGCC GCATTTGTGG AGAGGTACAT GAGAGAGTTT 
GGCTTCATTA TCCCCGAGCG GCCGGTGGTG GTAGATGATG TACGTGTGAG GGGAACTGGC 
CGTAGTGGAC TTCAGCTGGA GGACACCCCC AAAATCCAGA CTGGACCTCC CCACGTGGAA 
AAGGTGACCC AGTGCTACTT TGAAGGGGGT TATCAGGAGA CTCCCGTGTA CCTTTTAGGA 
GAACTAGGCT ACGGGCACCA GCTCCAAGGG CCCTGCCTTA TCATCGACAA CAACAGCACC 
ATCCTTGTAG AACCGGGTTG CCAAGCAGAG GTGACTGATA CAGGGGACAT CCGCATTTCT 
GTGGGAGCTG AGGGTCCTAG TATGGCAGAT ACCAGGCTTG ACCCCATCCA GCTGTCTATT 
TTCTCACACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTACA GCGCACAGCC 
ATCTCTACCA ACATCAAGGA ACGCCTCGAC TTCTCCTGTG CCCTCTTTGG GCCAGATGGG 
GGCCTCGTCT CCAATGCTCC CCACATTCCT GTGCACCTGG GTGCCATGCA AGAGACTGTA 
CAGTTCCAGA TTCAGCACTT AGGAGCCGAC CTCCATCCTG GTGATGTGTT GCTCAGCAAC 
CATCCCAGCG CAGGGGGCAG CCATCTTCCT GACCTGACTG TCATTACACC GGTGTTTTGG 
CCAGGCCAGA CGAGGCCTGT GTTCTACGTG GCTAGCCGAG GGCACCACGC AGACATTGGA 
GGAATCACAC CGGGCTCTAT GCCGCCTCAC TCCACCACGC TGCAACAGGA GGGTGCCGTT 
TTTCTGTCCT TCAAACTGGT CCAGGGAGGC GTCTTCCAGG AAGAGGCAGT GACAGAGGCC 
CTACGGGCAC CAGGCAAGAT CTCTGGCTGT AGTGGAACCA GGAACCTGCA TGACAACCTG 
TCGGATCTTC GTGCCCAGGT GGCAGCTAAC CAGAAAGGCA TCCAGCTGGT GGGAGAGCTG 
ATCGGACAGT ATGGCTTAGA TGTGGTGCAG GCCTATATGG GCCATATTCA GGCGAATGCT 
GAGCTAGCAG TGAGAGACAT GCTCCGGGCT TTTGGAACTT CCCGGCAGGC CAGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGATCACATG GATGATGGCT CTCCCATCTG TCTGCGTGTT 
CAGATCAACC TGAGTCAGGG CAGTGCGGTA TTTGACTTCA CTGGTTCCGG GTCTGAGGTG 
TTTGGCAATC TCAATGCCCC GAGAGCCATA ACACTGTCTG CTCTCATCTA TTGCTTACGC 
TGTCTAGTGG GCCGTGACAT CCCACTTAAC CAGGGTTGCC TGGCTCCTGT GCGTGTCATA 
ATTCCCAAAG GCTCCATATT GGATCCATCC CCAGAGGCAG CAGTGGTCGG CGGCAACGTG 
CTCACATCTC AGCGAGTAGT GGATGTCATT CTGGGGGCTT TTGGGGCCTG TTCAGCCTCC 
CAGGGCTGCA TGAACAATGT GACCCTGGGC AATGCCCGTA TGGGCTACTA TGAGACAGTG 
GCTGGTGGTG CCGGTGCGGG CCCTGGCTGG CATGGGCGCA GTGGTGTACA CAGTCACATG 
ACCAACACAC GCATTACGGA TCCAGAGATT CTGGAGAGTC GGTATCCAGT TATCCTGCGC 
CGCTTTGAGC TGAGGCCAGG CTCCGGGGGC CGAGGTCGCT TCCGGGGAGG TGATGGCGTA 
GTCCGAGAGC TGGTCTTTCG GGAAGAGGCG CTGTTGTCTG TGCTCACCGA GCGCCGGGCC 
TTCCAGCCTT ACGGCCTCCA CGGGGGAGAG CCTGGTGCGC GTGGCTTAAA CCTCCTGATC 
AGAAAAGATG GGCGCACAGT GAATTTGGGC GGCAAGACAT CTGTGACCGT GTACCCCGGG 
GACGTGTTCT GCCTCCACAC GCCTGGGGGT GGGGGCTACG GAGACCCGGA GGATCCAGCG 
CCACCACCAG GCTCGCCCCC GCTATTTCCA GCCTTCCCCG AGCGCGGCAG TGTATTCGAG 
TACCGCCGCG CCCAGGAAGC CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Oplah ( NM_053904.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446356.1 Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), mRNA.

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CloneID	ORa11013
Clone ID Related Accession (Same CDS sequence)	NM_053904.1
Accession Version	NM_053904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	5-oxoprolinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U70825.1. ##Evidence-Data-START## Transcript exon combination :: U70825.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAGCC CAGAAGGGCG CTTCCATTTC GCCATCGACC GCGGTGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCTGG AGGGCATGTG CGTGTCCTGA AGCTGCTCTC AGAGGACCCT 
GCCAACTATC CAGATGCACC CACAGAGGGC ATCCGCCGAA TTCTAGAGCA GGAGGAGGGT 
GTGCTGCTGC CTCGAGGCCG ACCGCTAGAC ACCAGTCGCA TTGCCAGCAT CCGCATGGGT 
ACCACGGTGG CCACCAATGC ACTGTTGGAA CGACAGGGAG AACGGGTGGC ACTGCTGGTG 
ACTCGGGGTT TCCGAGACCT GCTGCATATT GGCACTCAGG CCCGCCCGGA CCTCTTTGAC 
TTGGCTGTGC CCATGCCAGA GGTTCTGTAT GAGGAAGTGC TGGAGGTAGA TGAGCGAGTG 
GTGCTGTATC GCGGAGAACC AGGTGCCGGC TCTCCTGTCA AAGGCCGCAC AGGGGACCTG 
CTAGAGATAC AGCAGCCTGT GGACCTGGAA GCCCTGCGTG GGAAGCTGGA GGGGCTCTTG 
TCTCGGGGCA TTCACAGTCT GGCAGTGGTG CTCATGCATT CGTACACGTG GGCCCAGCAT 
GAGCAGCAGG TGGGCACGCT GGCCCGGGAG CTGGGCTTCA CGCACGTCTC CTTGTCCTCG 
GAAGTCATGC CCATGGTACG AATTGTTCCT CGGGGCCATA CAGCCTGTGC TGACGCTTAC 
CTTACTCCCA CCATCCAGCG CTATGTGCAG GGCTTCCGCC GAGGCTTCCA GGGCCAGCTA 
AAGAATGTGC AAGTTCTCTT CATGCGCTCT GATGGTGGCC TCGCACCCAT GGATGCTTTC 
AGTGGTTCCC GGGCTGTGCT CTCTGGCCCT GCTGGGGGTG TGGTTGGCTA CTCAGCTACC 
ACCTACCATC TGGAAGGCGG TCAGCCTGTC ATTGGCTTTG ACATGGGAGG CACATCCACA 
GACGTGAGCC GCTATGCTGG AGAATTTGAG CATGTCTTTG AGGCTAGCAC AGCAGGCGTT 
ACCCTTCAGG CACCCCAGTT GGACATCAAC ACAGTGGCAG CTGGCGGGGG TTCCCGCCTC 
TTCTTCAGAT CTGGCCTCTT TGTGGTTGGT CCAGAGTCAG CAGGTGCCCA CCCAGGTCCT 
GCCTGCTACC GTAAAGGGGG TCCTGTGACA GTGACAGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCAGGAG AAGACCAGCC ACTGTCTCCT 
GAGGCTTCCC GAAAGGCTCT AGAGGCTGTG GCCATGGAGG TCAACAGTTT CTTGACCAAT 
GGACCGTGCC CAGCTTCCCA ACTAAGTCTG GAAGAGGTGG CCATGGGGTT TGTGCGTGTT 
GCCAATGAAG CCATGTGCCG GCCTATCCGT GCCCTCACAC AGGCACGAGG CCATGACCCC 
TCAGCCCATG TATTGGCTTG CTTTGGAGGA GCTGGTGGGC AACACGCTTG TGCCATTGCC 
CGGGCCCTGG GGATGGATAC TGTGCACATT CACAGGCACA GCGGGCTGCT GTCAGCACTA 
GGACTGGCCT TGGCAGATGT GGTTCACGAA GCACAGGAGC CCTGTTCCCT GTCTTACACA 
CCTGAAACCT TTGCACAACT GGACCAGAGA CTGAGCCGCC TGGAGGAGCA GTGTGTGGAT 
GCCTTGCAGG TCCAGGGCTT CCCTAGGTCT CAGATCAGCA CCGAGAGCTT CCTGCATCTT 
CGCTACCAAG GCACTGACTG CGCCCTAATG GTGTCTGCCC ATCAGCATCC GGCCACAGCC 
TGCTCACCCC GAGCTGGTGA CTTTGGAGCC GCATTTGTGG AGAGGTACAT GAGAGAGTTT 
GGCTTCATTA TCCCCGAGCG GCCGGTGGTG GTAGATGATG TACGTGTGAG GGGAACTGGC 
CGTAGTGGAC TTCAGCTGGA GGACACCCCC AAAATCCAGA CTGGACCTCC CCACGTGGAA 
AAGGTGACCC AGTGCTACTT TGAAGGGGGT TATCAGGAGA CTCCCGTGTA CCTTTTAGGA 
GAACTAGGCT ACGGGCACCA GCTCCAAGGG CCCTGCCTTA TCATCGACAA CAACAGCACC 
ATCCTTGTAG AACCGGGTTG CCAAGCAGAG GTGACTGATA CAGGGGACAT CCGCATTTCT 
GTGGGAGCTG AGGGTCCTAG TATGGCAGAT ACCAGGCTTG ACCCCATCCA GCTGTCTATT 
TTCTCACACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTACA GCGCACAGCC 
ATCTCTACCA ACATCAAGGA ACGCCTCGAC TTCTCCTGTG CCCTCTTTGG GCCAGATGGG 
GGCCTCGTCT CCAATGCTCC CCACATTCCT GTGCACCTGG GTGCCATGCA AGAGACTGTA 
CAGTTCCAGA TTCAGCACTT AGGAGCCGAC CTCCATCCTG GTGATGTGTT GCTCAGCAAC 
CATCCCAGCG CAGGGGGCAG CCATCTTCCT GACCTGACTG TCATTACACC GGTGTTTTGG 
CCAGGCCAGA CGAGGCCTGT GTTCTACGTG GCTAGCCGAG GGCACCACGC AGACATTGGA 
GGAATCACAC CGGGCTCTAT GCCGCCTCAC TCCACCACGC TGCAACAGGA GGGTGCCGTT 
TTTCTGTCCT TCAAACTGGT CCAGGGAGGC GTCTTCCAGG AAGAGGCAGT GACAGAGGCC 
CTACGGGCAC CAGGCAAGAT CTCTGGCTGT AGTGGAACCA GGAACCTGCA TGACAACCTG 
TCGGATCTTC GTGCCCAGGT GGCAGCTAAC CAGAAAGGCA TCCAGCTGGT GGGAGAGCTG 
ATCGGACAGT ATGGCTTAGA TGTGGTGCAG GCCTATATGG GCCATATTCA GGCGAATGCT 
GAGCTAGCAG TGAGAGACAT GCTCCGGGCT TTTGGAACTT CCCGGCAGGC CAGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGATCACATG GATGATGGCT CTCCCATCTG TCTGCGTGTT 
CAGATCAACC TGAGTCAGGG CAGTGCGGTA TTTGACTTTA CTGGTTCCGG GTCTGAGGTG 
TTTGGCAATC TCAATGCCCC GAGAGCCATA ACACTGTCTG CTCTCATCTA TTGCTTACGC 
TGTCTAGTGG GCCGTGACAT CCCACTTAAC CAGGGTTGCC TGGCTCCTGT GCGTGTCATA 
ATTCCCAAAG GCTCCATATT GGATCCATCC CCAGAGGCAG CAGTGGTCGG CGGCAACGTG 
CTCACATCTC AGCGAGTAGT GGATGTCATT CTGGGGGCTT TTGGGGCCTG TTCAGCCTCC 
CAGGGCTGCA TGAACAATGT GACCCTGGGC AATGCCCGTA TGGGCTACTA TGAGACAGTG 
GCTGGTGGTG CCGGTGCGGG CCCTGGCTGG CATGGGCGCA GTGGTGTACA CAGTCACATG 
ACCAACACAC GCATTACGGA TCCAGAGATT CTGGAGAGTC GGTATCCAGT TATCCTGCGC 
CGCTTTGAGC TGAGGCCAGG CTCCGGGGGC CGAGGTCGCT TCCGGGGAGG TGATGGCGTA 
GTCCGAGAGC TGGTCTTTCG GGAAGAGGCG CTGTTGTCTG TGCTCACCGA GCGCCGGGCC 
TTCCAGCCTT ACGGCCTCCA CGGGGGAGAG CCTGGTGCGC GTGGCTTAAA CCTCCTGATC 
AGAAAAGATG GGCGCACAGT GAATTTGGGC GGCAAGACAT CTGTGACCGT GTACCCCGGG 
GACGTGTTCT GCCTCCACAC GCCTGGGGGT GGGGGCTACG GAGACCCGGA GGATCCAGCG 
CCACCACCAG GCTCGCCCCC GCTATTTCCA GCCTTCCCCG AGCGCGGCAG TGTATTCGAG 
TACCGCCGCG CCCAGGAAGC CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446356.1
CDS	106..3972
Translation	MGSPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYPDAPTEGIRRILEQEEGVLLPRGRPLDTSRIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDLFDLAVPMPEVLYEEVLEVDERVVLYRGEPGAGSPVKGRTGDLLEIQQPVDLEALRGKLEGLLSRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSLSSEVMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAFSGSRAVLSGPAGGVVGYSATTYHLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVAMEVNSFLTNGPCPASQLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLSYTPETFAQLDQRLSRLEEQCVDALQVQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPATACSPRAGDFGAAFVERYMREFGFIIPERPVVVDDVRVRGTGRSGLQLEDTPKIQTGPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNSTILVEPGCQAEVTDTGDIRISVGAEGPSMADTRLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKISGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGLPLEVSAEDHMDDGSPICLRVQINLSQGSAVFDFTGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVIIPKGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACSASQGCMNNVTLGNARMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELVFREEALLSVLTERRAFQPYGLHGGEPGARGLNLLIRKDGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPAPPPGSPPLFPAFPERGSVFEYRRAQEAV

Target ORF information:

RefSeq Version	NM_053904.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053904.1

ORF Insert Sequence:

ATGGGCAGCC CAGAAGGGCG CTTCCATTTC GCCATCGACC GCGGTGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCTGG AGGGCATGTG CGTGTCCTGA AGCTGCTCTC AGAGGACCCT 
GCCAACTATC CAGATGCACC CACAGAGGGC ATCCGCCGAA TTCTAGAGCA GGAGGAGGGT 
GTGCTGCTGC CTCGAGGCCG ACCGCTAGAC ACCAGTCGCA TTGCCAGCAT CCGCATGGGT 
ACCACGGTGG CCACCAATGC ACTGTTGGAA CGACAGGGAG AACGGGTGGC ACTGCTGGTG 
ACTCGGGGTT TCCGAGACCT GCTGCATATT GGCACTCAGG CCCGCCCGGA CCTCTTTGAC 
TTGGCTGTGC CCATGCCAGA GGTTCTGTAT GAGGAAGTGC TGGAGGTAGA TGAGCGAGTG 
GTGCTGTATC GCGGAGAACC AGGTGCCGGC TCTCCTGTCA AAGGCCGCAC AGGGGACCTG 
CTAGAGATAC AGCAGCCTGT GGACCTGGAA GCCCTGCGTG GGAAGCTGGA GGGGCTCTTG 
TCTCGGGGCA TTCACAGTCT GGCAGTGGTG CTCATGCATT CGTACACGTG GGCCCAGCAT 
GAGCAGCAGG TGGGCACGCT GGCCCGGGAG CTGGGCTTCA CGCACGTCTC CTTGTCCTCG 
GAAGTCATGC CCATGGTACG AATTGTTCCT CGGGGCCATA CAGCCTGTGC TGACGCTTAC 
CTTACTCCCA CCATCCAGCG CTATGTGCAG GGCTTCCGCC GAGGCTTCCA GGGCCAGCTA 
AAGAATGTGC AAGTTCTCTT CATGCGCTCT GATGGTGGCC TCGCACCCAT GGATGCTTTC 
AGTGGTTCCC GGGCTGTGCT CTCTGGCCCT GCTGGGGGTG TGGTTGGCTA CTCAGCTACC 
ACCTACCATC TGGAAGGCGG TCAGCCTGTC ATTGGCTTTG ACATGGGAGG CACATCCACA 
GACGTGAGCC GCTATGCTGG AGAATTTGAG CATGTCTTTG AGGCTAGCAC AGCAGGCGTT 
ACCCTTCAGG CACCCCAGTT GGACATCAAC ACAGTGGCAG CTGGCGGGGG TTCCCGCCTC 
TTCTTCAGAT CTGGCCTCTT TGTGGTTGGT CCAGAGTCAG CAGGTGCCCA CCCAGGTCCT 
GCCTGCTACC GTAAAGGGGG TCCTGTGACA GTGACAGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCAGGAG AAGACCAGCC ACTGTCTCCT 
GAGGCTTCCC GAAAGGCTCT AGAGGCTGTG GCCATGGAGG TCAACAGTTT CTTGACCAAT 
GGACCGTGCC CAGCTTCCCA ACTAAGTCTG GAAGAGGTGG CCATGGGGTT TGTGCGTGTT 
GCCAATGAAG CCATGTGCCG GCCTATCCGT GCCCTCACAC AGGCACGAGG CCATGACCCC 
TCAGCCCATG TATTGGCTTG CTTTGGAGGA GCTGGTGGGC AACACGCTTG TGCCATTGCC 
CGGGCCCTGG GGATGGATAC TGTGCACATT CACAGGCACA GCGGGCTGCT GTCAGCACTA 
GGACTGGCCT TGGCAGATGT GGTTCACGAA GCACAGGAGC CCTGTTCCCT GTCTTACACA 
CCTGAAACCT TTGCACAACT GGACCAGAGA CTGAGCCGCC TGGAGGAGCA GTGTGTGGAT 
GCCTTGCAGG TCCAGGGCTT CCCTAGGTCT CAGATCAGCA CCGAGAGCTT CCTGCATCTT 
CGCTACCAAG GCACTGACTG CGCCCTAATG GTGTCTGCCC ATCAGCATCC GGCCACAGCC 
TGCTCACCCC GAGCTGGTGA CTTTGGAGCC GCATTTGTGG AGAGGTACAT GAGAGAGTTT 
GGCTTCATTA TCCCCGAGCG GCCGGTGGTG GTAGATGATG TACGTGTGAG GGGAACTGGC 
CGTAGTGGAC TTCAGCTGGA GGACACCCCC AAAATCCAGA CTGGACCTCC CCACGTGGAA 
AAGGTGACCC AGTGCTACTT TGAAGGGGGT TATCAGGAGA CTCCCGTGTA CCTTTTAGGA 
GAACTAGGCT ACGGGCACCA GCTCCAAGGG CCCTGCCTTA TCATCGACAA CAACAGCACC 
ATCCTTGTAG AACCGGGTTG CCAAGCAGAG GTGACTGATA CAGGGGACAT CCGCATTTCT 
GTGGGAGCTG AGGGTCCTAG TATGGCAGAT ACCAGGCTTG ACCCCATCCA GCTGTCTATT 
TTCTCACACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTACA GCGCACAGCC 
ATCTCTACCA ACATCAAGGA ACGCCTCGAC TTCTCCTGTG CCCTCTTTGG GCCAGATGGG 
GGCCTCGTCT CCAATGCTCC CCACATTCCT GTGCACCTGG GTGCCATGCA AGAGACTGTA 
CAGTTCCAGA TTCAGCACTT AGGAGCCGAC CTCCATCCTG GTGATGTGTT GCTCAGCAAC 
CATCCCAGCG CAGGGGGCAG CCATCTTCCT GACCTGACTG TCATTACACC GGTGTTTTGG 
CCAGGCCAGA CGAGGCCTGT GTTCTACGTG GCTAGCCGAG GGCACCACGC AGACATTGGA 
GGAATCACAC CGGGCTCTAT GCCGCCTCAC TCCACCACGC TGCAACAGGA GGGTGCCGTT 
TTTCTGTCCT TCAAACTGGT CCAGGGAGGC GTCTTCCAGG AAGAGGCAGT GACAGAGGCC 
CTACGGGCAC CAGGCAAGAT CTCTGGCTGT AGTGGAACCA GGAACCTGCA TGACAACCTG 
TCGGATCTTC GTGCCCAGGT GGCAGCTAAC CAGAAAGGCA TCCAGCTGGT GGGAGAGCTG 
ATCGGACAGT ATGGCTTAGA TGTGGTGCAG GCCTATATGG GCCATATTCA GGCGAATGCT 
GAGCTAGCAG TGAGAGACAT GCTCCGGGCT TTTGGAACTT CCCGGCAGGC CAGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGATCACATG GATGATGGCT CTCCCATCTG TCTGCGTGTT 
CAGATCAACC TGAGTCAGGG CAGTGCGGTA TTTGACTTTA CTGGTTCCGG GTCTGAGGTG 
TTTGGCAATC TCAATGCCCC GAGAGCCATA ACACTGTCTG CTCTCATCTA TTGCTTACGC 
TGTCTAGTGG GCCGTGACAT CCCACTTAAC CAGGGTTGCC TGGCTCCTGT GCGTGTCATA 
ATTCCCAAAG GCTCCATATT GGATCCATCC CCAGAGGCAG CAGTGGTCGG CGGCAACGTG 
CTCACATCTC AGCGAGTAGT GGATGTCATT CTGGGGGCTT TTGGGGCCTG TTCAGCCTCC 
CAGGGCTGCA TGAACAATGT GACCCTGGGC AATGCCCGTA TGGGCTACTA TGAGACAGTG 
GCTGGTGGTG CCGGTGCGGG CCCTGGCTGG CATGGGCGCA GTGGTGTACA CAGTCACATG 
ACCAACACAC GCATTACGGA TCCAGAGATT CTGGAGAGTC GGTATCCAGT TATCCTGCGC 
CGCTTTGAGC TGAGGCCAGG CTCCGGGGGC CGAGGTCGCT TCCGGGGAGG TGATGGCGTA 
GTCCGAGAGC TGGTCTTTCG GGAAGAGGCG CTGTTGTCTG TGCTCACCGA GCGCCGGGCC 
TTCCAGCCTT ACGGCCTCCA CGGGGGAGAG CCTGGTGCGC GTGGCTTAAA CCTCCTGATC 
AGAAAAGATG GGCGCACAGT GAATTTGGGC GGCAAGACAT CTGTGACCGT GTACCCCGGG 
GACGTGTTCT GCCTCCACAC GCCTGGGGGT GGGGGCTACG GAGACCCGGA GGATCCAGCG 
CCACCACCAG GCTCGCCCCC GCTATTTCCA GCCTTCCCCG AGCGCGGCAG TGTATTCGAG 
TACCGCCGCG CCCAGGAAGC CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Oplah ( XM_006241771.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241833.1 Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X1, mRNA.

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CloneID	ORa30433
Clone ID Related Accession (Same CDS sequence)	XM_006241771.3
Accession Version	XM_006241771.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	5-oxoprolinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241771.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTTC TCAGCAGCTA TGAGGGCCTA CGACAGGAGA TACAGCGGCT GGCCCAAGAG 
AATGAGGAGC TACGGCGGCT AGTGCAGCTC ATCCAGGAAA ACCAAGAGCT AAAGCTAGTC 
CTTAGGAGCA GGGGCAATAG CCTAAGCTTA TGTGGCTCCA ACTTCCTGTC AGAAGCAACT 
GCCAACCCTA GGCTGCAGAA GCGTAAGACG ATCAGATTCA AGGATGTCGA GAGAGGTGAG 
CCACGGGCAT CCTTCAGAGC TCTCTCATCC AATGTGTGCC CAAACGGAAA GAAGCCATGC 
AAAACCCTGG GCCTGTGGAG TGTGGCCACC TGTTCCTCGT TGGGTGCTGG TGTTCTCCCA 
GGGTTACCAG CTGAAGAGCC ATTCCTGGAC TCCAGCTTCA ACGTCATGGG CAGCCCAGAA 
GGGCGCTTCC ATTTCGCCAT CGACCGCGGT GGCACCTTCA CAGATGTCTT TGCCCAGTGC 
CCTGGAGGGC ATGTGCGTGT CCTGAAGCTG CTCTCAGAGG ACCCTGCCAA CTATGCAGAT 
GCACCCACAG AGGGCATCCG CCGAATTCTA GAGCAGGAGG AGGGTGTGCT GCTGCCTCGA 
GGCCGACCGC TAGACACCAG TCGCATTGCC AGCATCCGCA TGGGTACCAC GGTGGCCACC 
AATGCACTGT TGGAACGACA GGGAGAACGG GTGGCACTGC TGGTGACTCG GGGTTTCCGA 
GACCTGCTGC ATATTGGCAC TCAGGCCCGC CCGGACCTCT TTGACTTGGC TGTGCCCATG 
CCAGAGGTTC TGTATGAGGA AGTGCTGGAG GTAGATGAGC GAGTGGTGCT GTATCGCGGA 
GAACCAGGTG CCGGCTCTCC TGTCAAAGGC CGCACAGGGG ACCTGCTAGA GATACAGCAG 
CCTGTGGACC TGGAAGCCCT GCGTGGGAAG CTGGAGGGGC TCTTGTCTCG GGGCATTCAC 
AGTCTGGCAG TGGTGCTCAT GCATTCGTAC ACGTGGGCCC AGCATGAGCA GCAGGTGGGC 
ACGCTGGCCC GGGAGCTGGG CTTCACGCAC GTCTCCTTGT CCTCGGAAGT CATGCCCATG 
GTACGAATTG TTCCTCGGGG CCATACAGCC TGTGCTGACG CTTACCTTAC TCCCACCATC 
CAGCGCTATG TGCAGGGCTT CCGCCGAGGC TTCCAGGGCC AGCTAAAGAA TGTGCAAGTT 
CTCTTCATGC GCTCTGATGG TGGCCTCGCA CCCATGGATG CTTTCAGTGG TTCCCGGGCT 
GTGCTCTCTG GCCCTGCTGG GGGTGTGGTT GGCTACTCAG CTACCACCTA CCATCTGGAA 
GGCGGTCAGC CTGTCATTGG CTTTGACATG GGAGGCACAT CCACAGACGT GAGCCGCTAT 
GCTGGAGAAT TTGAGCATGT CTTTGAGGCT AGCACAGCAG GCGTTACCCT TCAGGCACCC 
CAGTTGGACA TCAACACAGT GGCAGCTGGC GGGGGTTCCC GCCTCTTCTT CAGATCTGGC 
CTCTTTGTGG TTGGTCCAGA GTCAGCAGGT GCCCACCCAG GTCCTGCCTG CTACCGTAAA 
GGGGGTCCTG TGACAGTGAC AGATGCTAAT CTGGTCCTGG GTCGCCTGCT GCCTGCCTCC 
TTCCCCTGCA TTTTTGGGCC AGGAGAAGAC CAGCCACTGT CTCCTGAGGC TTCCCGAAAG 
GCTCTAGAGG CTGTGGCCAT GGAGGTCAAC AGTTTCTTGA CCAATGGACC GTGCCCAGCT 
TCCCAACTAA GTCTGGAAGA GGTGGCCATG GGGTTTGTGC GTGTTGCCAA TGAAGCCATG 
TGCCGGCCTA TCCGTGCCCT CACACAGGCA CGAGGCCATG ACCCCTCAGC CCATGTATTG 
GCTTGCTTTG GAGGAGCTGG TGGGCAACAC GCTTGTGCCA TTGCCCGGGC CCTGGGGATG 
GATACTGTGC ACATTCACAG GCACAGCGGG CTGCTGTCAG CACTAGGACT GGCCTTGGCA 
GATGTGGTTC ACGAAGCACA GGAGCCCTGT TCCCTGTCTT ACACACCTGA AACCTTTGCA 
CAACTGGACC AGAGACTGAG CCGCCTGGAG GAGCAGTGTG TGGATGCCTT GCAGGTCCAG 
GGCTTCCCTA GGTCTCAGAT CAGCACCGAG AGCTTCCTGC ATCTTCGCTA CCAAGGCACT 
GACTGCGCCC TAATGGTGTC TGCCCATCAG CATCCGGCCA CAGCCTGCTC ACCCCGTGCT 
GGTGACTTTG GAGCCGCATT TGTGGAGAGG TACATGAGAG AGTTTGGCTT CATTATCCCC 
GAGCGGCCGG TGGTGGTAGA TGATGTACGT GTGAGGGGAA CTGGCCGTAG TGGACTTCAG 
CTGGAGGACA CCCCCAAAAT CCAGACTGGA CCTCCCCACG TGGAAAAGGT GACCCAGTGC 
TACTTTGAAG GGGGTTATCA GGAGACTCCC GTGTACCTTT TAGGAGAACT AGGCTACGGG 
CACCAGCTCC AAGGGCCCTG CCTTATCATC GACAACAACA GCACCATCCT TGTAGAACCG 
GGTTGCCAAG CAGAGGTGAC TGATACAGGG GACATCCGCA TTTCTGTGGG AGCTGAGGGT 
CCTAGTATGG CAGATACCAG GCTTGACCCC ATCCAGCTGT CTATTTTCTC ACACCGCTTC 
ATGAGCATTG CTGAGCAGAT GGGCCGCATC CTACAGCGCA CAGCCATCTC TACCAACATC 
AAGGAACGCC TCGACTTCTC CTGTGCCCTC TTTGGGCCAG ATGGGGGCCT CGTCTCCAAT 
GCTCCCCACA TTCCTGTGCA CCTGGGTGCC ATGCAAGAGA CTGTACAGTT CCAGATTCAG 
CACTTAGGAG CCGACCTCCA TCCTGGTGAT GTGTTGCTCA GCAACCATCC CAGCGCAGGG 
GGCAGCCATC TTCCTGACCT GACTGTCATT ACACCGGTGT TTTGGCCAGG CCAGACGAGG 
CCTGTGTTCT ACGTGGCTAG CCGAGGGCAC CACGCAGACA TTGGAGGAAT CACACCGGGC 
TCTATGCCGC CTCACTCCAC CACGCTGCAA CAGGAGGGTG CCGTTTTTCT GTCCTTCAAA 
CTGGTCCAGG GAGGCGTCTT CCAGGAAGAG GCAGTGACAG AGGCCCTACG GGCACCAGGC 
AAGATCTCTG GCTGTAGTGG AACCAGGAAC CTGCATGACA ACCTGTCGGA TCTTCGTGCC 
CAGGTGGCAG CTAACCAGAA AGGCATCCAG CTGGTGGGAG AGCTGATCGG ACAGTATGGC 
TTAGATGTGG TGCAGGCCTA TATGGGCCAT ATTCAGGCGA ATGCTGAGCT AGCAGTGAGA 
GACATGCTCC GGGCTTTTGG AACTTCCCGG CAGGCCAGGG GCCTGCCCCT GGAGGTGTCT 
GCAGAGGATC ACATGGATGA TGGCTCTCCC ATCTGTCTGC GTGTTCAGAT CAACCTGAGT 
CAGGGCAGTG CGGTATTTGA CTTCACTGGT TCCGGGTCTG AGGTGTTTGG CAATCTCAAT 
GCCCCGAGAG CCATAACACT GTCTGCTCTC ATCTATTGCT TACGCTGTCT AGTGGGCCGT 
GACATCCCAC TTAACCAGGG TTGCCTGGCT CCTGTGCGTG TCATAATTCC CAAAGGCTCC 
ATATTGGATC CATCCCCAGA GGCAGCAGTG GTCGGCGGCA ACGTGCTCAC ATCTCAGCGA 
GTAGTGGATG TCATTCTGGG GGCTTTTGGG GCCTGTTCAG CCTCCCAGGG CTGCATGAAC 
AATGTGACCC TGGGCAATGC CCGTATGGGC TACTATGAGA CAGTGGCTGG TGGTGCCGGT 
GCGGGCCCTG GCTGGCATGG GCGCAGTGGT GTACACAGTC ACATGACCAA CACACGCATT 
ACGGATCCAG AGATTCTGGA GAGTCGGTAT CCAGTTATCC TGCGCCGCTT TGAGCTGAGG 
CCAGGCTCCG GGGGCCGAGG TCGCTTCCGG GGAGGTGATG GCGTAGTCCG AGAGCTGGTC 
TTTCGGGAAG AGGCGCTGTT GTCTGTGCTC ACCGAGCGCC GGGCCTTCCA GCCTTACGGC 
CTCCACGGGG GAGAGCCTGG TGCGCGTGGC TTAAACCTCC TGATCAGAAA AGATGGGCGC 
ACAGTGAATT TGGGCGGCAA GACATCTGTG ACCGTGTACC CCGGGGACGT GTTCTGCCTC 
CACACGCCTG GGGGTGGGGG CTACGGAGAC CCGGAGGATC CAGCGCCACC ACCAGGCTCG 
CCCCCGCTAT TTCCAGCCTT CCCCGAGCGC GGCAGTGTAT TCGAGTACCG CCGCGCCCAG 
GAAGCCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241833.1
CDS	1365..5636
Translation	MSLLSSYEGLRQEIQRLAQENEELRRLVQLIQENQELKLVLRSRGNSLSLCGSNFLSEATANPRLQKRKTIRFKDVERGEPRASFRALSSNVCPNGKKPCKTLGLWSVATCSSLGAGVLPGLPAEEPFLDSSFNVMGSPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRILEQEEGVLLPRGRPLDTSRIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDLFDLAVPMPEVLYEEVLEVDERVVLYRGEPGAGSPVKGRTGDLLEIQQPVDLEALRGKLEGLLSRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSLSSEVMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAFSGSRAVLSGPAGGVVGYSATTYHLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVAMEVNSFLTNGPCPASQLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLSYTPETFAQLDQRLSRLEEQCVDALQVQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPATACSPRAGDFGAAFVERYMREFGFIIPERPVVVDDVRVRGTGRSGLQLEDTPKIQTGPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNSTILVEPGCQAEVTDTGDIRISVGAEGPSMADTRLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKISGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGLPLEVSAEDHMDDGSPICLRVQINLSQGSAVFDFTGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVIIPKGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACSASQGCMNNVTLGNARMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELVFREEALLSVLTERRAFQPYGLHGGEPGARGLNLLIRKDGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPAPPPGSPPLFPAFPERGSVFEYRRAQEAV

Target ORF information:

RefSeq Version	XM_006241771.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241771.3

ORF Insert Sequence:

ATGTCCCTTC TCAGCAGCTA TGAGGGCCTA CGACAGGAGA TACAGCGGCT GGCCCAAGAG 
AATGAGGAGC TACGGCGGCT AGTGCAGCTC ATCCAGGAAA ACCAAGAGCT AAAGCTAGTC 
CTTAGGAGCA GGGGCAATAG CCTAAGCTTA TGTGGCTCCA ACTTCCTGTC AGAAGCAACT 
GCCAACCCTA GGCTGCAGAA GCGTAAGACG ATCAGATTCA AGGATGTCGA GAGAGGTGAG 
CCACGGGCAT CCTTCAGAGC TCTCTCATCC AATGTGTGCC CAAACGGAAA GAAGCCATGC 
AAAACCCTGG GCCTGTGGAG TGTGGCCACC TGTTCCTCGT TGGGTGCTGG TGTTCTCCCA 
GGGTTACCAG CTGAAGAGCC ATTCCTGGAC TCCAGCTTCA ACGTCATGGG CAGCCCAGAA 
GGGCGCTTCC ATTTCGCCAT CGACCGCGGT GGCACCTTCA CAGATGTCTT TGCCCAGTGC 
CCTGGAGGGC ATGTGCGTGT CCTGAAGCTG CTCTCAGAGG ACCCTGCCAA CTATGCAGAT 
GCACCCACAG AGGGCATCCG CCGAATTCTA GAGCAGGAGG AGGGTGTGCT GCTGCCTCGA 
GGCCGACCGC TAGACACCAG TCGCATTGCC AGCATCCGCA TGGGTACCAC GGTGGCCACC 
AATGCACTGT TGGAACGACA GGGAGAACGG GTGGCACTGC TGGTGACTCG GGGTTTCCGA 
GACCTGCTGC ATATTGGCAC TCAGGCCCGC CCGGACCTCT TTGACTTGGC TGTGCCCATG 
CCAGAGGTTC TGTATGAGGA AGTGCTGGAG GTAGATGAGC GAGTGGTGCT GTATCGCGGA 
GAACCAGGTG CCGGCTCTCC TGTCAAAGGC CGCACAGGGG ACCTGCTAGA GATACAGCAG 
CCTGTGGACC TGGAAGCCCT GCGTGGGAAG CTGGAGGGGC TCTTGTCTCG GGGCATTCAC 
AGTCTGGCAG TGGTGCTCAT GCATTCGTAC ACGTGGGCCC AGCATGAGCA GCAGGTGGGC 
ACGCTGGCCC GGGAGCTGGG CTTCACGCAC GTCTCCTTGT CCTCGGAAGT CATGCCCATG 
GTACGAATTG TTCCTCGGGG CCATACAGCC TGTGCTGACG CTTACCTTAC TCCCACCATC 
CAGCGCTATG TGCAGGGCTT CCGCCGAGGC TTCCAGGGCC AGCTAAAGAA TGTGCAAGTT 
CTCTTCATGC GCTCTGATGG TGGCCTCGCA CCCATGGATG CTTTCAGTGG TTCCCGGGCT 
GTGCTCTCTG GCCCTGCTGG GGGTGTGGTT GGCTACTCAG CTACCACCTA CCATCTGGAA 
GGCGGTCAGC CTGTCATTGG CTTTGACATG GGAGGCACAT CCACAGACGT GAGCCGCTAT 
GCTGGAGAAT TTGAGCATGT CTTTGAGGCT AGCACAGCAG GCGTTACCCT TCAGGCACCC 
CAGTTGGACA TCAACACAGT GGCAGCTGGC GGGGGTTCCC GCCTCTTCTT CAGATCTGGC 
CTCTTTGTGG TTGGTCCAGA GTCAGCAGGT GCCCACCCAG GTCCTGCCTG CTACCGTAAA 
GGGGGTCCTG TGACAGTGAC AGATGCTAAT CTGGTCCTGG GTCGCCTGCT GCCTGCCTCC 
TTCCCCTGCA TTTTTGGGCC AGGAGAAGAC CAGCCACTGT CTCCTGAGGC TTCCCGAAAG 
GCTCTAGAGG CTGTGGCCAT GGAGGTCAAC AGTTTCTTGA CCAATGGACC GTGCCCAGCT 
TCCCAACTAA GTCTGGAAGA GGTGGCCATG GGGTTTGTGC GTGTTGCCAA TGAAGCCATG 
TGCCGGCCTA TCCGTGCCCT CACACAGGCA CGAGGCCATG ACCCCTCAGC CCATGTATTG 
GCTTGCTTTG GAGGAGCTGG TGGGCAACAC GCTTGTGCCA TTGCCCGGGC CCTGGGGATG 
GATACTGTGC ACATTCACAG GCACAGCGGG CTGCTGTCAG CACTAGGACT GGCCTTGGCA 
GATGTGGTTC ACGAAGCACA GGAGCCCTGT TCCCTGTCTT ACACACCTGA AACCTTTGCA 
CAACTGGACC AGAGACTGAG CCGCCTGGAG GAGCAGTGTG TGGATGCCTT GCAGGTCCAG 
GGCTTCCCTA GGTCTCAGAT CAGCACCGAG AGCTTCCTGC ATCTTCGCTA CCAAGGCACT 
GACTGCGCCC TAATGGTGTC TGCCCATCAG CATCCGGCCA CAGCCTGCTC ACCCCGTGCT 
GGTGACTTTG GAGCCGCATT TGTGGAGAGG TACATGAGAG AGTTTGGCTT CATTATCCCC 
GAGCGGCCGG TGGTGGTAGA TGATGTACGT GTGAGGGGAA CTGGCCGTAG TGGACTTCAG 
CTGGAGGACA CCCCCAAAAT CCAGACTGGA CCTCCCCACG TGGAAAAGGT GACCCAGTGC 
TACTTTGAAG GGGGTTATCA GGAGACTCCC GTGTACCTTT TAGGAGAACT AGGCTACGGG 
CACCAGCTCC AAGGGCCCTG CCTTATCATC GACAACAACA GCACCATCCT TGTAGAACCG 
GGTTGCCAAG CAGAGGTGAC TGATACAGGG GACATCCGCA TTTCTGTGGG AGCTGAGGGT 
CCTAGTATGG CAGATACCAG GCTTGACCCC ATCCAGCTGT CTATTTTCTC ACACCGCTTC 
ATGAGCATTG CTGAGCAGAT GGGCCGCATC CTACAGCGCA CAGCCATCTC TACCAACATC 
AAGGAACGCC TCGACTTCTC CTGTGCCCTC TTTGGGCCAG ATGGGGGCCT CGTCTCCAAT 
GCTCCCCACA TTCCTGTGCA CCTGGGTGCC ATGCAAGAGA CTGTACAGTT CCAGATTCAG 
CACTTAGGAG CCGACCTCCA TCCTGGTGAT GTGTTGCTCA GCAACCATCC CAGCGCAGGG 
GGCAGCCATC TTCCTGACCT GACTGTCATT ACACCGGTGT TTTGGCCAGG CCAGACGAGG 
CCTGTGTTCT ACGTGGCTAG CCGAGGGCAC CACGCAGACA TTGGAGGAAT CACACCGGGC 
TCTATGCCGC CTCACTCCAC CACGCTGCAA CAGGAGGGTG CCGTTTTTCT GTCCTTCAAA 
CTGGTCCAGG GAGGCGTCTT CCAGGAAGAG GCAGTGACAG AGGCCCTACG GGCACCAGGC 
AAGATCTCTG GCTGTAGTGG AACCAGGAAC CTGCATGACA ACCTGTCGGA TCTTCGTGCC 
CAGGTGGCAG CTAACCAGAA AGGCATCCAG CTGGTGGGAG AGCTGATCGG ACAGTATGGC 
TTAGATGTGG TGCAGGCCTA TATGGGCCAT ATTCAGGCGA ATGCTGAGCT AGCAGTGAGA 
GACATGCTCC GGGCTTTTGG AACTTCCCGG CAGGCCAGGG GCCTGCCCCT GGAGGTGTCT 
GCAGAGGATC ACATGGATGA TGGCTCTCCC ATCTGTCTGC GTGTTCAGAT CAACCTGAGT 
CAGGGCAGTG CGGTATTTGA CTTCACTGGT TCCGGGTCTG AGGTGTTTGG CAATCTCAAT 
GCCCCGAGAG CCATAACACT GTCTGCTCTC ATCTATTGCT TACGCTGTCT AGTGGGCCGT 
GACATCCCAC TTAACCAGGG TTGCCTGGCT CCTGTGCGTG TCATAATTCC CAAAGGCTCC 
ATATTGGATC CATCCCCAGA GGCAGCAGTG GTCGGCGGCA ACGTGCTCAC ATCTCAGCGA 
GTAGTGGATG TCATTCTGGG GGCTTTTGGG GCCTGTTCAG CCTCCCAGGG CTGCATGAAC 
AATGTGACCC TGGGCAATGC CCGTATGGGC TACTATGAGA CAGTGGCTGG TGGTGCCGGT 
GCGGGCCCTG GCTGGCATGG GCGCAGTGGT GTACACAGTC ACATGACCAA CACACGCATT 
ACGGATCCAG AGATTCTGGA GAGTCGGTAT CCAGTTATCC TGCGCCGCTT TGAGCTGAGG 
CCAGGCTCCG GGGGCCGAGG TCGCTTCCGG GGAGGTGATG GCGTAGTCCG AGAGCTGGTC 
TTTCGGGAAG AGGCGCTGTT GTCTGTGCTC ACCGAGCGCC GGGCCTTCCA GCCTTACGGC 
CTCCACGGGG GAGAGCCTGG TGCGCGTGGC TTAAACCTCC TGATCAGAAA AGATGGGCGC 
ACAGTGAATT TGGGCGGCAA GACATCTGTG ACCGTGTACC CCGGGGACGT GTTCTGCCTC 
CACACGCCTG GGGGTGGGGG CTACGGAGAC CCGGAGGATC CAGCGCCACC ACCAGGCTCG 
CCCCCGCTAT TTCCAGCCTT CCCCGAGCGC GGCAGTGTAT TCGAGTACCG CCGCGCCCAG 
GAAGCCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Oplah ( XM_006241772.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241834.1 Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X2, mRNA.

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CloneID	ORa30434
Clone ID Related Accession (Same CDS sequence)	XM_006241772.3
Accession Version	XM_006241772.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	5-oxoprolinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241772.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTTC TCAGCAGCTA TGAGGGCCTA CGACAGGAGA TACAGCGGCT GGCCCAAGAG 
AATGAGGAGC TACGGCGGCT AGTGCAGCTC ATCCAGGAAA ACCAAGAGCT AAAGCTAGTC 
CTTAGGAGCA GGGGCAATAG CCTAAGCTTA TGTGGCTCCA ACTTCCTGTC AGAAGCAACT 
GCCAACCCTA GGCTGCAGAA GCGTAAGACG ATCAGATTCA AGGATGTCGA GAGAGGTGAG 
CCACGGGCAT CCTTCAGAGC TCTCTCATCC AATGTGTGCC CAAACGGAAA GAAGCCATGC 
AAAACCCTGG GCCTGTGGAG TGTGGCCACC TGTTCCTCGT TGGGTGCTGG TGTTCTCCCA 
GGGTTACCAG CTGAAGAGCC ATTCCTGGAC TCCAGCTTCA ACGTCATGGG CAGCCCAGAA 
GGGCGCTTCC ATTTCGCCAT CGACCGCGGT GGCACCTTCA CAGATGTCTT TGCCCAGTGC 
CCTGGAGGGC ATGTGCGTGT CCTGAAGCTG CTCTCAGAGG ACCCTGCCAA CTATGCAGAT 
GCACCCACAG AGGGCATCCG CCGAATTCTA GAGCAGGAGG AGGGTGTGCT GCTGCCTCGA 
GGCCGACCGC TAGACACCAG TCGCATTGCC AGCATCCGCA TGGGTACCAC GGTGGCCACC 
AATGCACTGT TGGAACGACA GGGAGAACGG GTGGCACTGC TGGTGACTCG GGGTTTCCGA 
GACCTGCTGC ATATTGGCAC TCAGGCCCGC CCGGACCTCT TTGACTTGGC TGTGCCCATG 
CCAGAGGTTC TGTATGAGGA AGTGCTGGAG GTAGATGAGC GAGTGGTGCT GTATCGCGGA 
GAACCAGGTG CCGGCTCTCC TGTCAAAGGC CGCACAGGGG ACCTGCTAGA GATACAGCAG 
CCTGTGGACC TGGAAGCCCT GCGTGGGAAG CTGGAGGGGC TCTTGTCTCG GGGCATTCAC 
AGTCTGGCAG TGGTGCTCAT GCATTCGTAC ACGTGGGCCC AGCATGAGCA GCAGGTGGGC 
ACGCTGGCCC GGGAGCTGGG CTTCACGCAC GTCTCCTTGT CCTCGGAAGT CATGCCCATG 
GTACGAATTG TTCCTCGGGG CCATACAGCC TGTGCTGACG CTTACCTTAC TCCCACCATC 
CAGCGCTATG TGCAGGGCTT CCGCCGAGGC TTCCAGGGCC AGCTAAAGAA TGTGCAAGTT 
CTCTTCATGC GCTCTGATGG TGGCCTCGCA CCCATGGATG CTTTCAGTGG TTCCCGGGCT 
GTGCTCTCTG GCCCTGCTGG GGGTGTGGTT GGCTACTCAG CTACCACCTA CCATCTGGAA 
GGCGGTCAGC CTGTCATTGG CTTTGACATG GGAGGCACAT CCACAGACGT GAGCCGCTAT 
GCTGGAGAAT TTGAGCATGT CTTTGAGGCT AGCACAGCAG GCGTTACCCT TCAGGCACCC 
CAGTTGGACA TCAACACAGT GGCAGCTGGC GGGGGTTCCC GCCTCTTCTT CAGATCTGGC 
CTCTTTGTGG TTGGTCCAGA GTCAGCAGGT GCCCACCCAG GTCCTGCCTG CTACCGTAAA 
GGGGGTCCTG TGACAGTGAC AGATGCTAAT CTGGTCCTGG GTCGCCTGCT GCCTGCCTCC 
TTCCCCTGCA TTTTTGGGCC AGGAGAAGAC CAGCCACTGT CTCCTGAGGC TTCCCGAAAG 
GCTCTAGAGG CTGTGGCCAT GGAGGTCAAC AGTTTCTTGA CCAATGGACC GTGCCCAGCT 
TCCCAACTAA GTCTGGAAGA GGTGGCCATG GGGTTTGTGC GTGTTGCCAA TGAAGCCATG 
TGCCGGCCTA TCCGTGCCCT CACACAGGCA CGAGGCCATG ACCCCTCAGC CCATGTATTG 
GCTTGCTTTG GAGGAGCTGG TGGGCAACAC GCTTGTGCCA TTGCCCGGGC CCTGGGGATG 
GATACTGTGC ACATTCACAG GCACAGCGGG CTGCTGTCAG CACTAGGACT GGCCTTGGCA 
GATGTGGTTC ACGAAGCACA GGAGCCCTGT TCCCTGTCTT ACACACCTGA AACCTTTGCA 
CAACTGGACC AGAGACTGAG CCGCCTGGAG GAGCAGTGTG TGGATGCCTT GCAGGTCCAG 
GGCTTCCCTA GGTCTCAGAT CAGCACCGAG AGCTTCCTGC ATCTTCGCTA CCAAGGCACT 
GACTGCGCCC TAATGGTGTC TGCCCATCAG CATCCGGCCA CAGCCTGCTC ACCCCGTGCT 
GGTGACTTTG GAGCCGCATT TGTGGAGAGG TACATGAGAG AGTTTGGCTT CATTATCCCC 
GAGCGGCCGG TGGTGGTAGA TGATGTACGT GTGAGGGGAA CTGGCCGTAG TGGACTTCAG 
CTGGAGGACA CCCCCAAAAT CCAGACTGGA CCTCCCCACG TGGAAAAGGT GACCCAGTGC 
TACTTTGAAG GGGGTTATCA GGAGACTCCC GTGTACCTTT TAGGAGAACT AGGCTACGGG 
CACCAGCTCC AAGGGCCCTG CCTTATCATC GACAACAACA GCACCATCCT TGTAGAACCG 
GGTTGCCAAG CAGAGGTGAC TGATACAGGG GACATCCGCA TTTCTGTGGG AGCTGAGGGT 
CCTAGTATGG CAGATACCAG GCTTGACCCC ATCCAGCTGT CTATTTTCTC ACACCGCTTC 
ATGAGCATTG CTGAGCAGAT GGGCCGCATC CTACAGCGCA CAGCCATCTC TACCAACATC 
AAGGAACGCC TCGACTTCTC CTGTGCCCTC TTTGGGCCAG ATGGGGGCCT CGTCTCCAAT 
GCTCCCCACA TTCCTGTGCA CCTGGGTGCC ATGCAAGAGA CTGTACAGTT CCAGATTCAG 
CACTTAGGAG CCGACCTCCA TCCTGGTGAT GTGTTGCTCA GCAACCATCC CAGCGCAGGG 
GGCAGCCATC TTCCTGACCT GACTGTCATT ACACCGGTGT TTTGGCCAGG CCAGACGAGG 
CCTGTGTTCT ACGTGGCTAG CCGAGGGCAC CACGCAGACA TTGGAGGAAT CACACCGGGC 
TCTATGCCGC CTCACTCCAC CACGCTGCAA CAGGAGGGTG CCGTTTTTCT GTCCTTCAAA 
CTGGTCCAGG GAGGCGTCTT CCAGGAAGAG GTGACAGAGG CCCTACGGGC ACCAGGCAAG 
ATCTCTGGCT GTAGTGGAAC CAGGAACCTG CATGACAACC TGTCGGATCT TCGTGCCCAG 
GTGGCAGCTA ACCAGAAAGG CATCCAGCTG GTGGGAGAGC TGATCGGACA GTATGGCTTA 
GATGTGGTGC AGGCCTATAT GGGCCATATT CAGGCGAATG CTGAGCTAGC AGTGAGAGAC 
ATGCTCCGGG CTTTTGGAAC TTCCCGGCAG GCCAGGGGCC TGCCCCTGGA GGTGTCTGCA 
GAGGATCACA TGGATGATGG CTCTCCCATC TGTCTGCGTG TTCAGATCAA CCTGAGTCAG 
GGCAGTGCGG TATTTGACTT CACTGGTTCC GGGTCTGAGG TGTTTGGCAA TCTCAATGCC 
CCGAGAGCCA TAACACTGTC TGCTCTCATC TATTGCTTAC GCTGTCTAGT GGGCCGTGAC 
ATCCCACTTA ACCAGGGTTG CCTGGCTCCT GTGCGTGTCA TAATTCCCAA AGGCTCCATA 
TTGGATCCAT CCCCAGAGGC AGCAGTGGTC GGCGGCAACG TGCTCACATC TCAGCGAGTA 
GTGGATGTCA TTCTGGGGGC TTTTGGGGCC TGTTCAGCCT CCCAGGGCTG CATGAACAAT 
GTGACCCTGG GCAATGCCCG TATGGGCTAC TATGAGACAG TGGCTGGTGG TGCCGGTGCG 
GGCCCTGGCT GGCATGGGCG CAGTGGTGTA CACAGTCACA TGACCAACAC ACGCATTACG 
GATCCAGAGA TTCTGGAGAG TCGGTATCCA GTTATCCTGC GCCGCTTTGA GCTGAGGCCA 
GGCTCCGGGG GCCGAGGTCG CTTCCGGGGA GGTGATGGCG TAGTCCGAGA GCTGGTCTTT 
CGGGAAGAGG CGCTGTTGTC TGTGCTCACC GAGCGCCGGG CCTTCCAGCC TTACGGCCTC 
CACGGGGGAG AGCCTGGTGC GCGTGGCTTA AACCTCCTGA TCAGAAAAGA TGGGCGCACA 
GTGAATTTGG GCGGCAAGAC ATCTGTGACC GTGTACCCCG GGGACGTGTT CTGCCTCCAC 
ACGCCTGGGG GTGGGGGCTA CGGAGACCCG GAGGATCCAG CGCCACCACC AGGCTCGCCC 
CCGCTATTTC CAGCCTTCCC CGAGCGCGGC AGTGTATTCG AGTACCGCCG CGCCCAGGAA 
GCCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241834.1
CDS	1365..5633
Translation	MSLLSSYEGLRQEIQRLAQENEELRRLVQLIQENQELKLVLRSRGNSLSLCGSNFLSEATANPRLQKRKTIRFKDVERGEPRASFRALSSNVCPNGKKPCKTLGLWSVATCSSLGAGVLPGLPAEEPFLDSSFNVMGSPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRILEQEEGVLLPRGRPLDTSRIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDLFDLAVPMPEVLYEEVLEVDERVVLYRGEPGAGSPVKGRTGDLLEIQQPVDLEALRGKLEGLLSRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSLSSEVMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAFSGSRAVLSGPAGGVVGYSATTYHLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVAMEVNSFLTNGPCPASQLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLSYTPETFAQLDQRLSRLEEQCVDALQVQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPATACSPRAGDFGAAFVERYMREFGFIIPERPVVVDDVRVRGTGRSGLQLEDTPKIQTGPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNSTILVEPGCQAEVTDTGDIRISVGAEGPSMADTRLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVFQEEVTEALRAPGKISGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGLPLEVSAEDHMDDGSPICLRVQINLSQGSAVFDFTGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVIIPKGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACSASQGCMNNVTLGNARMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELVFREEALLSVLTERRAFQPYGLHGGEPGARGLNLLIRKDGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPAPPPGSPPLFPAFPERGSVFEYRRAQEAV

Target ORF information:

RefSeq Version	XM_006241772.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241772.3

ORF Insert Sequence:

ATGTCCCTTC TCAGCAGCTA TGAGGGCCTA CGACAGGAGA TACAGCGGCT GGCCCAAGAG 
AATGAGGAGC TACGGCGGCT AGTGCAGCTC ATCCAGGAAA ACCAAGAGCT AAAGCTAGTC 
CTTAGGAGCA GGGGCAATAG CCTAAGCTTA TGTGGCTCCA ACTTCCTGTC AGAAGCAACT 
GCCAACCCTA GGCTGCAGAA GCGTAAGACG ATCAGATTCA AGGATGTCGA GAGAGGTGAG 
CCACGGGCAT CCTTCAGAGC TCTCTCATCC AATGTGTGCC CAAACGGAAA GAAGCCATGC 
AAAACCCTGG GCCTGTGGAG TGTGGCCACC TGTTCCTCGT TGGGTGCTGG TGTTCTCCCA 
GGGTTACCAG CTGAAGAGCC ATTCCTGGAC TCCAGCTTCA ACGTCATGGG CAGCCCAGAA 
GGGCGCTTCC ATTTCGCCAT CGACCGCGGT GGCACCTTCA CAGATGTCTT TGCCCAGTGC 
CCTGGAGGGC ATGTGCGTGT CCTGAAGCTG CTCTCAGAGG ACCCTGCCAA CTATGCAGAT 
GCACCCACAG AGGGCATCCG CCGAATTCTA GAGCAGGAGG AGGGTGTGCT GCTGCCTCGA 
GGCCGACCGC TAGACACCAG TCGCATTGCC AGCATCCGCA TGGGTACCAC GGTGGCCACC 
AATGCACTGT TGGAACGACA GGGAGAACGG GTGGCACTGC TGGTGACTCG GGGTTTCCGA 
GACCTGCTGC ATATTGGCAC TCAGGCCCGC CCGGACCTCT TTGACTTGGC TGTGCCCATG 
CCAGAGGTTC TGTATGAGGA AGTGCTGGAG GTAGATGAGC GAGTGGTGCT GTATCGCGGA 
GAACCAGGTG CCGGCTCTCC TGTCAAAGGC CGCACAGGGG ACCTGCTAGA GATACAGCAG 
CCTGTGGACC TGGAAGCCCT GCGTGGGAAG CTGGAGGGGC TCTTGTCTCG GGGCATTCAC 
AGTCTGGCAG TGGTGCTCAT GCATTCGTAC ACGTGGGCCC AGCATGAGCA GCAGGTGGGC 
ACGCTGGCCC GGGAGCTGGG CTTCACGCAC GTCTCCTTGT CCTCGGAAGT CATGCCCATG 
GTACGAATTG TTCCTCGGGG CCATACAGCC TGTGCTGACG CTTACCTTAC TCCCACCATC 
CAGCGCTATG TGCAGGGCTT CCGCCGAGGC TTCCAGGGCC AGCTAAAGAA TGTGCAAGTT 
CTCTTCATGC GCTCTGATGG TGGCCTCGCA CCCATGGATG CTTTCAGTGG TTCCCGGGCT 
GTGCTCTCTG GCCCTGCTGG GGGTGTGGTT GGCTACTCAG CTACCACCTA CCATCTGGAA 
GGCGGTCAGC CTGTCATTGG CTTTGACATG GGAGGCACAT CCACAGACGT GAGCCGCTAT 
GCTGGAGAAT TTGAGCATGT CTTTGAGGCT AGCACAGCAG GCGTTACCCT TCAGGCACCC 
CAGTTGGACA TCAACACAGT GGCAGCTGGC GGGGGTTCCC GCCTCTTCTT CAGATCTGGC 
CTCTTTGTGG TTGGTCCAGA GTCAGCAGGT GCCCACCCAG GTCCTGCCTG CTACCGTAAA 
GGGGGTCCTG TGACAGTGAC AGATGCTAAT CTGGTCCTGG GTCGCCTGCT GCCTGCCTCC 
TTCCCCTGCA TTTTTGGGCC AGGAGAAGAC CAGCCACTGT CTCCTGAGGC TTCCCGAAAG 
GCTCTAGAGG CTGTGGCCAT GGAGGTCAAC AGTTTCTTGA CCAATGGACC GTGCCCAGCT 
TCCCAACTAA GTCTGGAAGA GGTGGCCATG GGGTTTGTGC GTGTTGCCAA TGAAGCCATG 
TGCCGGCCTA TCCGTGCCCT CACACAGGCA CGAGGCCATG ACCCCTCAGC CCATGTATTG 
GCTTGCTTTG GAGGAGCTGG TGGGCAACAC GCTTGTGCCA TTGCCCGGGC CCTGGGGATG 
GATACTGTGC ACATTCACAG GCACAGCGGG CTGCTGTCAG CACTAGGACT GGCCTTGGCA 
GATGTGGTTC ACGAAGCACA GGAGCCCTGT TCCCTGTCTT ACACACCTGA AACCTTTGCA 
CAACTGGACC AGAGACTGAG CCGCCTGGAG GAGCAGTGTG TGGATGCCTT GCAGGTCCAG 
GGCTTCCCTA GGTCTCAGAT CAGCACCGAG AGCTTCCTGC ATCTTCGCTA CCAAGGCACT 
GACTGCGCCC TAATGGTGTC TGCCCATCAG CATCCGGCCA CAGCCTGCTC ACCCCGTGCT 
GGTGACTTTG GAGCCGCATT TGTGGAGAGG TACATGAGAG AGTTTGGCTT CATTATCCCC 
GAGCGGCCGG TGGTGGTAGA TGATGTACGT GTGAGGGGAA CTGGCCGTAG TGGACTTCAG 
CTGGAGGACA CCCCCAAAAT CCAGACTGGA CCTCCCCACG TGGAAAAGGT GACCCAGTGC 
TACTTTGAAG GGGGTTATCA GGAGACTCCC GTGTACCTTT TAGGAGAACT AGGCTACGGG 
CACCAGCTCC AAGGGCCCTG CCTTATCATC GACAACAACA GCACCATCCT TGTAGAACCG 
GGTTGCCAAG CAGAGGTGAC TGATACAGGG GACATCCGCA TTTCTGTGGG AGCTGAGGGT 
CCTAGTATGG CAGATACCAG GCTTGACCCC ATCCAGCTGT CTATTTTCTC ACACCGCTTC 
ATGAGCATTG CTGAGCAGAT GGGCCGCATC CTACAGCGCA CAGCCATCTC TACCAACATC 
AAGGAACGCC TCGACTTCTC CTGTGCCCTC TTTGGGCCAG ATGGGGGCCT CGTCTCCAAT 
GCTCCCCACA TTCCTGTGCA CCTGGGTGCC ATGCAAGAGA CTGTACAGTT CCAGATTCAG 
CACTTAGGAG CCGACCTCCA TCCTGGTGAT GTGTTGCTCA GCAACCATCC CAGCGCAGGG 
GGCAGCCATC TTCCTGACCT GACTGTCATT ACACCGGTGT TTTGGCCAGG CCAGACGAGG 
CCTGTGTTCT ACGTGGCTAG CCGAGGGCAC CACGCAGACA TTGGAGGAAT CACACCGGGC 
TCTATGCCGC CTCACTCCAC CACGCTGCAA CAGGAGGGTG CCGTTTTTCT GTCCTTCAAA 
CTGGTCCAGG GAGGCGTCTT CCAGGAAGAG GTGACAGAGG CCCTACGGGC ACCAGGCAAG 
ATCTCTGGCT GTAGTGGAAC CAGGAACCTG CATGACAACC TGTCGGATCT TCGTGCCCAG 
GTGGCAGCTA ACCAGAAAGG CATCCAGCTG GTGGGAGAGC TGATCGGACA GTATGGCTTA 
GATGTGGTGC AGGCCTATAT GGGCCATATT CAGGCGAATG CTGAGCTAGC AGTGAGAGAC 
ATGCTCCGGG CTTTTGGAAC TTCCCGGCAG GCCAGGGGCC TGCCCCTGGA GGTGTCTGCA 
GAGGATCACA TGGATGATGG CTCTCCCATC TGTCTGCGTG TTCAGATCAA CCTGAGTCAG 
GGCAGTGCGG TATTTGACTT CACTGGTTCC GGGTCTGAGG TGTTTGGCAA TCTCAATGCC 
CCGAGAGCCA TAACACTGTC TGCTCTCATC TATTGCTTAC GCTGTCTAGT GGGCCGTGAC 
ATCCCACTTA ACCAGGGTTG CCTGGCTCCT GTGCGTGTCA TAATTCCCAA AGGCTCCATA 
TTGGATCCAT CCCCAGAGGC AGCAGTGGTC GGCGGCAACG TGCTCACATC TCAGCGAGTA 
GTGGATGTCA TTCTGGGGGC TTTTGGGGCC TGTTCAGCCT CCCAGGGCTG CATGAACAAT 
GTGACCCTGG GCAATGCCCG TATGGGCTAC TATGAGACAG TGGCTGGTGG TGCCGGTGCG 
GGCCCTGGCT GGCATGGGCG CAGTGGTGTA CACAGTCACA TGACCAACAC ACGCATTACG 
GATCCAGAGA TTCTGGAGAG TCGGTATCCA GTTATCCTGC GCCGCTTTGA GCTGAGGCCA 
GGCTCCGGGG GCCGAGGTCG CTTCCGGGGA GGTGATGGCG TAGTCCGAGA GCTGGTCTTT 
CGGGAAGAGG CGCTGTTGTC TGTGCTCACC GAGCGCCGGG CCTTCCAGCC TTACGGCCTC 
CACGGGGGAG AGCCTGGTGC GCGTGGCTTA AACCTCCTGA TCAGAAAAGA TGGGCGCACA 
GTGAATTTGG GCGGCAAGAC ATCTGTGACC GTGTACCCCG GGGACGTGTT CTGCCTCCAC 
ACGCCTGGGG GTGGGGGCTA CGGAGACCCG GAGGATCCAG CGCCACCACC AGGCTCGCCC 
CCGCTATTTC CAGCCTTCCC CGAGCGCGGC AGTGTATTCG AGTACCGCCG CGCCCAGGAA 
GCCGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Oplah ( XM_006241773.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241835.1 Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X3, mRNA.

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CloneID	ORa30435
Clone ID Related Accession (Same CDS sequence)	XM_006241773.3
Accession Version	XM_006241773.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	5-oxoprolinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241773.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTTC TCAGCAGCTA TGAGGGCCTA CGACAGGAGA TACAGCGGCT GGCCCAAGAG 
AATGAGGAGC TACGGCGGCT AGTGCAGCTC ATCCAGGAAA ACCAAGAGCT AAAGCTAGTC 
CTTAGGAGCA GGGGCAATAG CCTAAGCTTA TGTGGCTCCA ACTTCCTGTC AGAAGCAACT 
GCCAACCCTA GGCTGCAGAA GCGTAAGACG ATCAGATTCA AGGATGTCGA GAGAGTTCTC 
CCAGGGTTAC CAGCTGAAGA GCCATTCCTG GACTCCAGCT TCAACGTCAT GGGCAGCCCA 
GAAGGGCGCT TCCATTTCGC CATCGACCGC GGTGGCACCT TCACAGATGT CTTTGCCCAG 
TGCCCTGGAG GGCATGTGCG TGTCCTGAAG CTGCTCTCAG AGGACCCTGC CAACTATGCA 
GATGCACCCA CAGAGGGCAT CCGCCGAATT CTAGAGCAGG AGGAGGGTGT GCTGCTGCCT 
CGAGGCCGAC CGCTAGACAC CAGTCGCATT GCCAGCATCC GCATGGGTAC CACGGTGGCC 
ACCAATGCAC TGTTGGAACG ACAGGGAGAA CGGGTGGCAC TGCTGGTGAC TCGGGGTTTC 
CGAGACCTGC TGCATATTGG CACTCAGGCC CGCCCGGACC TCTTTGACTT GGCTGTGCCC 
ATGCCAGAGG TTCTGTATGA GGAAGTGCTG GAGGTAGATG AGCGAGTGGT GCTGTATCGC 
GGAGAACCAG GTGCCGGCTC TCCTGTCAAA GGCCGCACAG GGGACCTGCT AGAGATACAG 
CAGCCTGTGG ACCTGGAAGC CCTGCGTGGG AAGCTGGAGG GGCTCTTGTC TCGGGGCATT 
CACAGTCTGG CAGTGGTGCT CATGCATTCG TACACGTGGG CCCAGCATGA GCAGCAGGTG 
GGCACGCTGG CCCGGGAGCT GGGCTTCACG CACGTCTCCT TGTCCTCGGA AGTCATGCCC 
ATGGTACGAA TTGTTCCTCG GGGCCATACA GCCTGTGCTG ACGCTTACCT TACTCCCACC 
ATCCAGCGCT ATGTGCAGGG CTTCCGCCGA GGCTTCCAGG GCCAGCTAAA GAATGTGCAA 
GTTCTCTTCA TGCGCTCTGA TGGTGGCCTC GCACCCATGG ATGCTTTCAG TGGTTCCCGG 
GCTGTGCTCT CTGGCCCTGC TGGGGGTGTG GTTGGCTACT CAGCTACCAC CTACCATCTG 
GAAGGCGGTC AGCCTGTCAT TGGCTTTGAC ATGGGAGGCA CATCCACAGA CGTGAGCCGC 
TATGCTGGAG AATTTGAGCA TGTCTTTGAG GCTAGCACAG CAGGCGTTAC CCTTCAGGCA 
CCCCAGTTGG ACATCAACAC AGTGGCAGCT GGCGGGGGTT CCCGCCTCTT CTTCAGATCT 
GGCCTCTTTG TGGTTGGTCC AGAGTCAGCA GGTGCCCACC CAGGTCCTGC CTGCTACCGT 
AAAGGGGGTC CTGTGACAGT GACAGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC 
TCCTTCCCCT GCATTTTTGG GCCAGGAGAA GACCAGCCAC TGTCTCCTGA GGCTTCCCGA 
AAGGCTCTAG AGGCTGTGGC CATGGAGGTC AACAGTTTCT TGACCAATGG ACCGTGCCCA 
GCTTCCCAAC TAAGTCTGGA AGAGGTGGCC ATGGGGTTTG TGCGTGTTGC CAATGAAGCC 
ATGTGCCGGC CTATCCGTGC CCTCACACAG GCACGAGGCC ATGACCCCTC AGCCCATGTA 
TTGGCTTGCT TTGGAGGAGC TGGTGGGCAA CACGCTTGTG CCATTGCCCG GGCCCTGGGG 
ATGGATACTG TGCACATTCA CAGGCACAGC GGGCTGCTGT CAGCACTAGG ACTGGCCTTG 
GCAGATGTGG TTCACGAAGC ACAGGAGCCC TGTTCCCTGT CTTACACACC TGAAACCTTT 
GCACAACTGG ACCAGAGACT GAGCCGCCTG GAGGAGCAGT GTGTGGATGC CTTGCAGGTC 
CAGGGCTTCC CTAGGTCTCA GATCAGCACC GAGAGCTTCC TGCATCTTCG CTACCAAGGC 
ACTGACTGCG CCCTAATGGT GTCTGCCCAT CAGCATCCGG CCACAGCCTG CTCACCCCGT 
GCTGGTGACT TTGGAGCCGC ATTTGTGGAG AGGTACATGA GAGAGTTTGG CTTCATTATC 
CCCGAGCGGC CGGTGGTGGT AGATGATGTA CGTGTGAGGG GAACTGGCCG TAGTGGACTT 
CAGCTGGAGG ACACCCCCAA AATCCAGACT GGACCTCCCC ACGTGGAAAA GGTGACCCAG 
TGCTACTTTG AAGGGGGTTA TCAGGAGACT CCCGTGTACC TTTTAGGAGA ACTAGGCTAC 
GGGCACCAGC TCCAAGGGCC CTGCCTTATC ATCGACAACA ACAGCACCAT CCTTGTAGAA 
CCGGGTTGCC AAGCAGAGGT GACTGATACA GGGGACATCC GCATTTCTGT GGGAGCTGAG 
GGTCCTAGTA TGGCAGATAC CAGGCTTGAC CCCATCCAGC TGTCTATTTT CTCACACCGC 
TTCATGAGCA TTGCTGAGCA GATGGGCCGC ATCCTACAGC GCACAGCCAT CTCTACCAAC 
ATCAAGGAAC GCCTCGACTT CTCCTGTGCC CTCTTTGGGC CAGATGGGGG CCTCGTCTCC 
AATGCTCCCC ACATTCCTGT GCACCTGGGT GCCATGCAAG AGACTGTACA GTTCCAGATT 
CAGCACTTAG GAGCCGACCT CCATCCTGGT GATGTGTTGC TCAGCAACCA TCCCAGCGCA 
GGGGGCAGCC ATCTTCCTGA CCTGACTGTC ATTACACCGG TGTTTTGGCC AGGCCAGACG 
AGGCCTGTGT TCTACGTGGC TAGCCGAGGG CACCACGCAG ACATTGGAGG AATCACACCG 
GGCTCTATGC CGCCTCACTC CACCACGCTG CAACAGGAGG GTGCCGTTTT TCTGTCCTTC 
AAACTGGTCC AGGGAGGCGT CTTCCAGGAA GAGGCAGTGA CAGAGGCCCT ACGGGCACCA 
GGCAAGATCT CTGGCTGTAG TGGAACCAGG AACCTGCATG ACAACCTGTC GGATCTTCGT 
GCCCAGGTGG CAGCTAACCA GAAAGGCATC CAGCTGGTGG GAGAGCTGAT CGGACAGTAT 
GGCTTAGATG TGGTGCAGGC CTATATGGGC CATATTCAGG CGAATGCTGA GCTAGCAGTG 
AGAGACATGC TCCGGGCTTT TGGAACTTCC CGGCAGGCCA GGGGCCTGCC CCTGGAGGTG 
TCTGCAGAGG ATCACATGGA TGATGGCTCT CCCATCTGTC TGCGTGTTCA GATCAACCTG 
AGTCAGGGCA GTGCGGTATT TGACTTCACT GGTTCCGGGT CTGAGGTGTT TGGCAATCTC 
AATGCCCCGA GAGCCATAAC ACTGTCTGCT CTCATCTATT GCTTACGCTG TCTAGTGGGC 
CGTGACATCC CACTTAACCA GGGTTGCCTG GCTCCTGTGC GTGTCATAAT TCCCAAAGGC 
TCCATATTGG ATCCATCCCC AGAGGCAGCA GTGGTCGGCG GCAACGTGCT CACATCTCAG 
CGAGTAGTGG ATGTCATTCT GGGGGCTTTT GGGGCCTGTT CAGCCTCCCA GGGCTGCATG 
AACAATGTGA CCCTGGGCAA TGCCCGTATG GGCTACTATG AGACAGTGGC TGGTGGTGCC 
GGTGCGGGCC CTGGCTGGCA TGGGCGCAGT GGTGTACACA GTCACATGAC CAACACACGC 
ATTACGGATC CAGAGATTCT GGAGAGTCGG TATCCAGTTA TCCTGCGCCG CTTTGAGCTG 
AGGCCAGGCT CCGGGGGCCG AGGTCGCTTC CGGGGAGGTG ATGGCGTAGT CCGAGAGCTG 
GTCTTTCGGG AAGAGGCGCT GTTGTCTGTG CTCACCGAGC GCCGGGCCTT CCAGCCTTAC 
GGCCTCCACG GGGGAGAGCC TGGTGCGCGT GGCTTAAACC TCCTGATCAG AAAAGATGGG 
CGCACAGTGA ATTTGGGCGG CAAGACATCT GTGACCGTGT ACCCCGGGGA CGTGTTCTGC 
CTCCACACGC CTGGGGGTGG GGGCTACGGA GACCCGGAGG ATCCAGCGCC ACCACCAGGC 
TCGCCCCCGC TATTTCCAGC CTTCCCCGAG CGCGGCAGTG TATTCGAGTA CCGCCGCGCC 
CAGGAAGCCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241835.1
CDS	1365..5519
Translation	MSLLSSYEGLRQEIQRLAQENEELRRLVQLIQENQELKLVLRSRGNSLSLCGSNFLSEATANPRLQKRKTIRFKDVERVLPGLPAEEPFLDSSFNVMGSPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRILEQEEGVLLPRGRPLDTSRIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDLFDLAVPMPEVLYEEVLEVDERVVLYRGEPGAGSPVKGRTGDLLEIQQPVDLEALRGKLEGLLSRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSLSSEVMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAFSGSRAVLSGPAGGVVGYSATTYHLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVAMEVNSFLTNGPCPASQLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLSYTPETFAQLDQRLSRLEEQCVDALQVQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPATACSPRAGDFGAAFVERYMREFGFIIPERPVVVDDVRVRGTGRSGLQLEDTPKIQTGPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNSTILVEPGCQAEVTDTGDIRISVGAEGPSMADTRLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKISGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGLPLEVSAEDHMDDGSPICLRVQINLSQGSAVFDFTGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVIIPKGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACSASQGCMNNVTLGNARMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELVFREEALLSVLTERRAFQPYGLHGGEPGARGLNLLIRKDGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPAPPPGSPPLFPAFPERGSVFEYRRAQEAV

Target ORF information:

RefSeq Version	XM_006241773.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 5-oxoprolinase (ATP-hydrolysing) (Oplah), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241773.3

ORF Insert Sequence:

ATGTCCCTTC TCAGCAGCTA TGAGGGCCTA CGACAGGAGA TACAGCGGCT GGCCCAAGAG 
AATGAGGAGC TACGGCGGCT AGTGCAGCTC ATCCAGGAAA ACCAAGAGCT AAAGCTAGTC 
CTTAGGAGCA GGGGCAATAG CCTAAGCTTA TGTGGCTCCA ACTTCCTGTC AGAAGCAACT 
GCCAACCCTA GGCTGCAGAA GCGTAAGACG ATCAGATTCA AGGATGTCGA GAGAGTTCTC 
CCAGGGTTAC CAGCTGAAGA GCCATTCCTG GACTCCAGCT TCAACGTCAT GGGCAGCCCA 
GAAGGGCGCT TCCATTTCGC CATCGACCGC GGTGGCACCT TCACAGATGT CTTTGCCCAG 
TGCCCTGGAG GGCATGTGCG TGTCCTGAAG CTGCTCTCAG AGGACCCTGC CAACTATGCA 
GATGCACCCA CAGAGGGCAT CCGCCGAATT CTAGAGCAGG AGGAGGGTGT GCTGCTGCCT 
CGAGGCCGAC CGCTAGACAC CAGTCGCATT GCCAGCATCC GCATGGGTAC CACGGTGGCC 
ACCAATGCAC TGTTGGAACG ACAGGGAGAA CGGGTGGCAC TGCTGGTGAC TCGGGGTTTC 
CGAGACCTGC TGCATATTGG CACTCAGGCC CGCCCGGACC TCTTTGACTT GGCTGTGCCC 
ATGCCAGAGG TTCTGTATGA GGAAGTGCTG GAGGTAGATG AGCGAGTGGT GCTGTATCGC 
GGAGAACCAG GTGCCGGCTC TCCTGTCAAA GGCCGCACAG GGGACCTGCT AGAGATACAG 
CAGCCTGTGG ACCTGGAAGC CCTGCGTGGG AAGCTGGAGG GGCTCTTGTC TCGGGGCATT 
CACAGTCTGG CAGTGGTGCT CATGCATTCG TACACGTGGG CCCAGCATGA GCAGCAGGTG 
GGCACGCTGG CCCGGGAGCT GGGCTTCACG CACGTCTCCT TGTCCTCGGA AGTCATGCCC 
ATGGTACGAA TTGTTCCTCG GGGCCATACA GCCTGTGCTG ACGCTTACCT TACTCCCACC 
ATCCAGCGCT ATGTGCAGGG CTTCCGCCGA GGCTTCCAGG GCCAGCTAAA GAATGTGCAA 
GTTCTCTTCA TGCGCTCTGA TGGTGGCCTC GCACCCATGG ATGCTTTCAG TGGTTCCCGG 
GCTGTGCTCT CTGGCCCTGC TGGGGGTGTG GTTGGCTACT CAGCTACCAC CTACCATCTG 
GAAGGCGGTC AGCCTGTCAT TGGCTTTGAC ATGGGAGGCA CATCCACAGA CGTGAGCCGC 
TATGCTGGAG AATTTGAGCA TGTCTTTGAG GCTAGCACAG CAGGCGTTAC CCTTCAGGCA 
CCCCAGTTGG ACATCAACAC AGTGGCAGCT GGCGGGGGTT CCCGCCTCTT CTTCAGATCT 
GGCCTCTTTG TGGTTGGTCC AGAGTCAGCA GGTGCCCACC CAGGTCCTGC CTGCTACCGT 
AAAGGGGGTC CTGTGACAGT GACAGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC 
TCCTTCCCCT GCATTTTTGG GCCAGGAGAA GACCAGCCAC TGTCTCCTGA GGCTTCCCGA 
AAGGCTCTAG AGGCTGTGGC CATGGAGGTC AACAGTTTCT TGACCAATGG ACCGTGCCCA 
GCTTCCCAAC TAAGTCTGGA AGAGGTGGCC ATGGGGTTTG TGCGTGTTGC CAATGAAGCC 
ATGTGCCGGC CTATCCGTGC CCTCACACAG GCACGAGGCC ATGACCCCTC AGCCCATGTA 
TTGGCTTGCT TTGGAGGAGC TGGTGGGCAA CACGCTTGTG CCATTGCCCG GGCCCTGGGG 
ATGGATACTG TGCACATTCA CAGGCACAGC GGGCTGCTGT CAGCACTAGG ACTGGCCTTG 
GCAGATGTGG TTCACGAAGC ACAGGAGCCC TGTTCCCTGT CTTACACACC TGAAACCTTT 
GCACAACTGG ACCAGAGACT GAGCCGCCTG GAGGAGCAGT GTGTGGATGC CTTGCAGGTC 
CAGGGCTTCC CTAGGTCTCA GATCAGCACC GAGAGCTTCC TGCATCTTCG CTACCAAGGC 
ACTGACTGCG CCCTAATGGT GTCTGCCCAT CAGCATCCGG CCACAGCCTG CTCACCCCGT 
GCTGGTGACT TTGGAGCCGC ATTTGTGGAG AGGTACATGA GAGAGTTTGG CTTCATTATC 
CCCGAGCGGC CGGTGGTGGT AGATGATGTA CGTGTGAGGG GAACTGGCCG TAGTGGACTT 
CAGCTGGAGG ACACCCCCAA AATCCAGACT GGACCTCCCC ACGTGGAAAA GGTGACCCAG 
TGCTACTTTG AAGGGGGTTA TCAGGAGACT CCCGTGTACC TTTTAGGAGA ACTAGGCTAC 
GGGCACCAGC TCCAAGGGCC CTGCCTTATC ATCGACAACA ACAGCACCAT CCTTGTAGAA 
CCGGGTTGCC AAGCAGAGGT GACTGATACA GGGGACATCC GCATTTCTGT GGGAGCTGAG 
GGTCCTAGTA TGGCAGATAC CAGGCTTGAC CCCATCCAGC TGTCTATTTT CTCACACCGC 
TTCATGAGCA TTGCTGAGCA GATGGGCCGC ATCCTACAGC GCACAGCCAT CTCTACCAAC 
ATCAAGGAAC GCCTCGACTT CTCCTGTGCC CTCTTTGGGC CAGATGGGGG CCTCGTCTCC 
AATGCTCCCC ACATTCCTGT GCACCTGGGT GCCATGCAAG AGACTGTACA GTTCCAGATT 
CAGCACTTAG GAGCCGACCT CCATCCTGGT GATGTGTTGC TCAGCAACCA TCCCAGCGCA 
GGGGGCAGCC ATCTTCCTGA CCTGACTGTC ATTACACCGG TGTTTTGGCC AGGCCAGACG 
AGGCCTGTGT TCTACGTGGC TAGCCGAGGG CACCACGCAG ACATTGGAGG AATCACACCG 
GGCTCTATGC CGCCTCACTC CACCACGCTG CAACAGGAGG GTGCCGTTTT TCTGTCCTTC 
AAACTGGTCC AGGGAGGCGT CTTCCAGGAA GAGGCAGTGA CAGAGGCCCT ACGGGCACCA 
GGCAAGATCT CTGGCTGTAG TGGAACCAGG AACCTGCATG ACAACCTGTC GGATCTTCGT 
GCCCAGGTGG CAGCTAACCA GAAAGGCATC CAGCTGGTGG GAGAGCTGAT CGGACAGTAT 
GGCTTAGATG TGGTGCAGGC CTATATGGGC CATATTCAGG CGAATGCTGA GCTAGCAGTG 
AGAGACATGC TCCGGGCTTT TGGAACTTCC CGGCAGGCCA GGGGCCTGCC CCTGGAGGTG 
TCTGCAGAGG ATCACATGGA TGATGGCTCT CCCATCTGTC TGCGTGTTCA GATCAACCTG 
AGTCAGGGCA GTGCGGTATT TGACTTCACT GGTTCCGGGT CTGAGGTGTT TGGCAATCTC 
AATGCCCCGA GAGCCATAAC ACTGTCTGCT CTCATCTATT GCTTACGCTG TCTAGTGGGC 
CGTGACATCC CACTTAACCA GGGTTGCCTG GCTCCTGTGC GTGTCATAAT TCCCAAAGGC 
TCCATATTGG ATCCATCCCC AGAGGCAGCA GTGGTCGGCG GCAACGTGCT CACATCTCAG 
CGAGTAGTGG ATGTCATTCT GGGGGCTTTT GGGGCCTGTT CAGCCTCCCA GGGCTGCATG 
AACAATGTGA CCCTGGGCAA TGCCCGTATG GGCTACTATG AGACAGTGGC TGGTGGTGCC 
GGTGCGGGCC CTGGCTGGCA TGGGCGCAGT GGTGTACACA GTCACATGAC CAACACACGC 
ATTACGGATC CAGAGATTCT GGAGAGTCGG TATCCAGTTA TCCTGCGCCG CTTTGAGCTG 
AGGCCAGGCT CCGGGGGCCG AGGTCGCTTC CGGGGAGGTG ATGGCGTAGT CCGAGAGCTG 
GTCTTTCGGG AAGAGGCGCT GTTGTCTGTG CTCACCGAGC GCCGGGCCTT CCAGCCTTAC 
GGCCTCCACG GGGGAGAGCC TGGTGCGCGT GGCTTAAACC TCCTGATCAG AAAAGATGGG 
CGCACAGTGA ATTTGGGCGG CAAGACATCT GTGACCGTGT ACCCCGGGGA CGTGTTCTGC 
CTCCACACGC CTGGGGGTGG GGGCTACGGA GACCCGGAGG ATCCAGCGCC ACCACCAGGC 
TCGCCCCCGC TATTTCCAGC CTTCCCCGAG CGCGGCAGTG TATTCGAGTA CCGCCGCGCC 
CAGGAAGCCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amino acid sequence of rat kidney 5-oxo-L-prolinase determined by cDNA cloning.
The Journal of biological chemistry271(50)32293-300(1996 Dec)
Ye GJ,Breslow EB,Meister A,Guo-jie GE

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Oplah cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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