Slc28a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc28a1
Entrez Gene ID	116642
Full Name	solute carrier family 28 member 1
Synonyms	Cnt1
General protein information	Preferred Names solute carrier family 28 member 1 Names sodium/nucleoside cotransporter 1 CNT 1 Na(+)/nucleoside cotransporter 1 concentrative nucleoside transporter 1 sodium-coupled nucleoside transporter 1 solute carrier family 28 (concentrative nucleoside transporter), member 1 solute carrier family 28 (sodium-coupled nucleoside transporter), member 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q31
Summary	sodium dependent nucleoside transporter [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053863.1	NP_446315.1	sodium/nucleoside cotransporter 1
XM_006229423.3	XP_006229485.1	sodium/nucleoside cotransporter 1 isoform X1
XM_006229422.3	XP_006229484.1	sodium/nucleoside cotransporter 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC28A1	NP_004204.3
Canis lupus familiaris (dog)	SLC28A1	XP_005618786.1
Pan troglodytes (chimpanzee)	SLC28A1	XP_003952772.1
	SLC28A1	XP_002804972.1
Mus musculus (house mouse)	Slc28a1	NP_001004184.2
Rattus norvegicus (Norway rat)	Slc28a1	NP_446315.1
Bos taurus (cattle)	SLC28A1	NP_001032535.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of concentrative nucleoside transporters SLC28 (CNT1, CNT2, and CNT3) along the rat nephron: effect of diabetes.
Rodr?guez-Mulero S, Errasti-Murugarren E, Ballar?n J, Felipe A, Doucet A, Casado FJ, Pastor-Anglada M
Kidney international68(2)665-72(2005 Aug)

Concentrative nucleoside transporter (rCNT1) is targeted to the apical membrane through the hepatic transcytotic pathway.
Duflot S, Calvo M, Casado FJ, Enrich C, Pastor-Anglada M
Experimental cell research281(1)77-85(2002 Nov)

Cell-cycle-dependent regulation of CNT1, a concentrative nucleoside transporter involved in the uptake of cell-cycle-dependent nucleoside-derived anticancer drugs.
Vald?s R, Casado FJ, Pastor-Anglada M
Biochemical and biophysical research communications296(3)575-9(2002 Aug)

Na+-dependent nucleoside transport in liver: two different isoforms from the same gene family are expressed in liver cells.
Felipe A, Valdes R, Santo B, Lloberas J, Casado J, Pastor-Anglada M
The Biochemical journal330 ( Pt 2)997-1001(1998 Mar)

Stable expression of a recombinant sodium-dependent, pyrimidine-selective nucleoside transporter (CNT1) in a transport-deficient mouse leukemia cell line.
Crawford CR, Cass CE, Young JD, Belt JA
Biochemistry and cell biology = Biochimie et biologie cellulaire76(5)843-51(1998)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Diabetes increased CNT1 and CNT3 expression in the glomerulus and insulin treatment decreased it
Title: Expression of concentrative nucleoside transporters SLC28 (CNT1, CNT2, and CNT3) along the rat nephron: effect of diabetes.

Cell-cycle-dependent regulation of CNT1 protein levels in a rat hepatoma cell line.
Title: Cell-cycle-dependent regulation of CNT1, a concentrative nucleoside transporter involved in the uptake of cell-cycle-dependent nucleoside-derived anticancer drugs.

The following Slc28a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc28a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10887	NM_053863.1 Latest version!	Rattus norvegicus solute carrier family 28 member 1 (Slc28a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$384.30 $549.00
ORa22931	XM_006229422.3 Latest version!	Rattus norvegicus solute carrier family 28 member 1 (Slc28a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa22931	XM_006229423.3 Latest version!	Rattus norvegicus solute carrier family 28 member 1 (Slc28a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc28a1 ( NM_053863.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446315.1 Rattus norvegicus solute carrier family 28 member 1 (Slc28a1), mRNA.

Price & Availability↑

CloneID	ORa10887
Clone ID Related Accession (Same CDS sequence)	NM_053863.1
Accession Version	NM_053863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	sodium/nucleoside cotransporter 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U10279.1. ##Evidence-Data-START## Transcript exon combination :: U10279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642767, SAMN02642775 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGACA ACACACAGAG GCAAAGAGAG TCCATTTCCC TCACGCCTAT GGCCCACGGC 
CTGGAGAACA TGGGGGCAGA ATTCCTGGAA AGCATGGAGG AAGGCCGACT CCCTCACAGT 
CACTCAAGCC TGCCGGAGGG TGAAGGTGGC CTGAACAAAG CAGAGCGGAA GGCCTTCTCC 
CGATGGAGGA GTCTGCAGCC GACTGTGCAA GCGAGAAGCT TCTGCAGGGA GCACCGGCAG 
CTGTTTGGAT GGATCTGCAA AGGCCTGCTC TCTACTGCAT GTCTTGGCTT CTTGATGGTC 
GCCTGCCTCC TGGACCTCCA GAGGGCCCTA GCACTGTTGA TCATCACCTG TGTGGTTCTC 
GTCTTTCTGG CCTATGATCT GCTAAAGAGG CTTCTGGGGT CCAAGCTGAG GAGGTGTGTG 
AAGTTTCAAG GCCATTCTTG CCTGAGCCTC TGGCTGAAAA GAGGTCTAGC CCTTGCTGCT 
GGTGTGGGCC TGATCTTGTG GCTATCTCTG GACACCGCCC AGCGGCCTGA ACAGCTGGTG 
TCCTTTGCAG GGATCTGTGT GTTCCTTGTC CTTCTCTTTG CTGGCTCAAA GCATCACCGT 
GCGGTGTCAT GGCGAGCTGT GTCCTGGGGC CTTGGGCTGC AGTTTGTGCT TGGGCTCTTC 
GTCATCAGAA CAGAACCAGG GTTCATTGCA TTCCAGTGGC TAGGGGATCA GATCCAGGTC 
TTCCTGAGTT ACACCGAGGC AGGCTCCAGC TTCGTCTTCG GAGAGGCTCT GGTGAAGGAT 
GTCTTTGCCT TTCAGGTTTT GCCCATCATC ATCTTCTTCA GCTGCGTCAT GTCTGTTCTG 
TACTATCTGG GCCTCATGCA GTGGGTGATC CTGAAGATTG CCTGGTTGAT GCAGGTCACC 
ATGGGCACCT CAGCCACCGA GACACTGAGT GTGGCGGGAA ACATCTTTGT GAGCCAGACT 
GAAGCTCCTC TGCTGATCCG GCCCTATCTG GCAGACATGA CACTCTCTGA AGTTCACGTT 
GTCATGACTG GAGGCTATGC TACCATTGCT GGCAGCCTCC TGGGCGCCTA CATCTCCTTT 
GGGATCGACG CTGCTTCCTT AATCGCAGCC TCTGTCATGG CCGCCCCTTG TGCGTTGGCT 
CTCTCCAAGC TGGTCTACCC AGAGGTGGAG GAGTCCAAGT TCCGGAGTGA GAATGGCGTG 
AAGCTGACCT ATGGAGACGC TCAGAACCTC TTGGAAGCAG CCAGTGCTGG GGCTGCCATC 
TCAGTGAAGG TCGTTGCCAA CATTGCTGCC AATCTGATTG CCTTCCTGGC TGTACTAGCC 
TTCGTCAATG CTGCCCTCTC CTGGCTAGGG GACATGGTGG ACATCCAGGG ACTCAGCTTC 
CAGCTCATCT GCTCCTACGT CCTGCGGCCT GTGGCCTTCT TGATGGGTGT GGCCTGGGAG 
GACTGTCCGG TAGTGGCTGA GTTGCTGGGC ATCAAGTTCT TTCTGAATGA GTTTGTGGCC 
TATCAAGAGC TTTCCCAGTA CAAGCAACGA CGCCTGGCAG GGGCTGAGGA GTGGCTTGGT 
GACAAGAAAC AGTGGATCTC TGTCAGAGCA GAAATCCTGA CTACATACGC CCTCTGTGGA 
TTTGCCAACT TCAGCTCCAT CGGCATCATG TTGGGAGGCC TGACCTCCCT AGTCCCCCAG 
CGGAGGAGCG ACTTCTCCCA GATTGTACTC CGGGCACTGA TCACAGGGGC TTTCGTGTCC 
CTGCTAAACG CCTGTGTGGC AGGGATCCTC TATGTACCCA GGGGGGTCGA GGTGGACTGC 
GTGTCCCTTC TGAACCAAAC TGTCAGCAGC AGCAGCTTTG AGGTTTACCT GTGCTGCCGC 
CAAGTCTTCC AGAGCACTAG CTCGGAGTTC AGCCAAGTGG CACTGGACAA CTGCTGTCGA 
TTTTACAACC ACACAGTCTG CACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446315.1
CDS	157..2103
Translation	MADNTQRQRESISLTPMAHGLENMGAEFLESMEEGRLPHSHSSLPEGEGGLNKAERKAFSRWRSLQPTVQARSFCREHRQLFGWICKGLLSTACLGFLMVACLLDLQRALALLIITCVVLVFLAYDLLKRLLGSKLRRCVKFQGHSCLSLWLKRGLALAAGVGLILWLSLDTAQRPEQLVSFAGICVFLVLLFAGSKHHRAVSWRAVSWGLGLQFVLGLFVIRTEPGFIAFQWLGDQIQVFLSYTEAGSSFVFGEALVKDVFAFQVLPIIIFFSCVMSVLYYLGLMQWVILKIAWLMQVTMGTSATETLSVAGNIFVSQTEAPLLIRPYLADMTLSEVHVVMTGGYATIAGSLLGAYISFGIDAASLIAASVMAAPCALALSKLVYPEVEESKFRSENGVKLTYGDAQNLLEAASAGAAISVKVVANIAANLIAFLAVLAFVNAALSWLGDMVDIQGLSFQLICSYVLRPVAFLMGVAWEDCPVVAELLGIKFFLNEFVAYQELSQYKQRRLAGAEEWLGDKKQWISVRAEILTTYALCGFANFSSIGIMLGGLTSLVPQRRSDFSQIVLRALITGAFVSLLNACVAGILYVPRGVEVDCVSLLNQTVSSSSFEVYLCCRQVFQSTSSEFSQVALDNCCRFYNHTVCT

Target ORF information:

RefSeq Version	NM_053863.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 28 member 1 (Slc28a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053863.1

ORF Insert Sequence:

ATGGCAGACA ACACACAGAG GCAAAGAGAG TCCATTTCCC TCACGCCTAT GGCCCACGGC 
CTGGAGAACA TGGGGGCAGA ATTCCTGGAA AGCATGGAGG AAGGCCGACT CCCTCACAGT 
CACTCAAGCC TGCCGGAGGG TGAAGGTGGC CTGAACAAAG CAGAGCGGAA GGCCTTCTCC 
CGATGGAGGA GTCTGCAGCC GACTGTGCAA GCGAGAAGCT TCTGCAGGGA GCACCGGCAG 
CTGTTTGGAT GGATCTGCAA AGGCCTGCTC TCTACTGCAT GTCTTGGCTT CTTGATGGTC 
GCCTGCCTCC TGGACCTCCA GAGGGCCCTA GCACTGTTGA TCATCACCTG TGTGGTTCTC 
GTCTTTCTGG CCTATGATCT GCTAAAGAGG CTTCTGGGGT CCAAGCTGAG GAGGTGTGTG 
AAGTTTCAAG GCCATTCTTG CCTGAGCCTC TGGCTGAAAA GAGGTCTAGC CCTTGCTGCT 
GGTGTGGGCC TGATCTTGTG GCTATCTCTG GACACCGCCC AGCGGCCTGA ACAGCTGGTG 
TCCTTTGCAG GGATCTGTGT GTTCCTTGTC CTTCTCTTTG CTGGCTCAAA GCATCACCGT 
GCGGTGTCAT GGCGAGCTGT GTCCTGGGGC CTTGGGCTGC AGTTTGTGCT TGGGCTCTTC 
GTCATCAGAA CAGAACCAGG GTTCATTGCA TTCCAGTGGC TAGGGGATCA GATCCAGGTC 
TTCCTGAGTT ACACCGAGGC AGGCTCCAGC TTCGTCTTCG GAGAGGCTCT GGTGAAGGAT 
GTCTTTGCCT TTCAGGTTTT GCCCATCATC ATCTTCTTCA GCTGCGTCAT GTCTGTTCTG 
TACTATCTGG GCCTCATGCA GTGGGTGATC CTGAAGATTG CCTGGTTGAT GCAGGTCACC 
ATGGGCACCT CAGCCACCGA GACACTGAGT GTGGCGGGAA ACATCTTTGT GAGCCAGACT 
GAAGCTCCTC TGCTGATCCG GCCCTATCTG GCAGACATGA CACTCTCTGA AGTTCACGTT 
GTCATGACTG GAGGCTATGC TACCATTGCT GGCAGCCTCC TGGGCGCCTA CATCTCCTTT 
GGGATCGACG CTGCTTCCTT AATCGCAGCC TCTGTCATGG CCGCCCCTTG TGCGTTGGCT 
CTCTCCAAGC TGGTCTACCC AGAGGTGGAG GAGTCCAAGT TCCGGAGTGA GAATGGCGTG 
AAGCTGACCT ATGGAGACGC TCAGAACCTC TTGGAAGCAG CCAGTGCTGG GGCTGCCATC 
TCAGTGAAGG TCGTTGCCAA CATTGCTGCC AATCTGATTG CCTTCCTGGC TGTACTAGCC 
TTCGTCAATG CTGCCCTCTC CTGGCTAGGG GACATGGTGG ACATCCAGGG ACTCAGCTTC 
CAGCTCATCT GCTCCTACGT CCTGCGGCCT GTGGCCTTCT TGATGGGTGT GGCCTGGGAG 
GACTGTCCGG TAGTGGCTGA GTTGCTGGGC ATCAAGTTCT TTCTGAATGA GTTTGTGGCC 
TATCAAGAGC TTTCCCAGTA CAAGCAACGA CGCCTGGCAG GGGCTGAGGA GTGGCTTGGT 
GACAAGAAAC AGTGGATCTC TGTCAGAGCA GAAATCCTGA CTACATACGC CCTCTGTGGA 
TTTGCCAACT TCAGCTCCAT CGGCATCATG TTGGGAGGCC TGACCTCCCT AGTCCCCCAG 
CGGAGGAGCG ACTTCTCCCA GATTGTACTC CGGGCACTGA TCACAGGGGC TTTCGTGTCC 
CTGCTAAACG CCTGTGTGGC AGGGATCCTC TATGTACCCA GGGGGGTCGA GGTGGACTGC 
GTGTCCCTTC TGAACCAAAC TGTCAGCAGC AGCAGCTTTG AGGTTTACCT GTGCTGCCGC 
CAAGTCTTCC AGAGCACTAG CTCGGAGTTC AGCCAAGTGG CACTGGACAA CTGCTGTCGA 
TTTTACAACC ACACAGTCTG CACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc28a1 ( XM_006229423.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229485.1 Rattus norvegicus solute carrier family 28 member 1 (Slc28a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa22931
Clone ID Related Accession (Same CDS sequence)	XM_006229423.3 , XM_006229422.3
Accession Version	XM_006229423.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium/nucleoside cotransporter 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229423.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACA ACACACAGAG GCAAAGAGAG TCCATTTCCC TCACGCCTAT GGCCCACGGC 
CTGGAGAACA TGGGGGCAGA ATTCCTGGAA AGCATGGAGG AAGGCCGACT CCCTCACAGT 
CACTCAAGCC TGCCGGAGGG TGAAGGTGGC CTGAACAAAG CAGAGCGGAA GGCCTTCTCC 
CGATGGAGGA GTCTGCAGCC GACTGTGCAA GCGAGAAGCT TCTGCAGGGA GCACCGGCAG 
CTGTTTGGAT GGATCTGCAA AGGCCTGCTC TCTACTGCAT GTCTTGGCTT CTTGATGGTC 
GCCTGCCTCC TGGACCTCCA GAGGGCCCTA GCACTGTTGA TCATCACCTG TGTGGTTCTC 
GTCTTTCTGG CCTATGATCT GCTAAAGAGG CTTCTGGGGT CCAAGCTGAG GAGGTGTGTG 
AAGTTTCAAG GCCATTCTTG CCTGAGCCTC TGGCTGAAAA GAGGTCTAGC CCTTGCTGCT 
GGTGTGGGCC TGATCTTGTG GCTATCTCTG GACACCGCCC AGCGGCCTGA ACAGCTGGTG 
TCCTTTGCAG GGATCTGTGT GTTCCTTGTC CTTCTCTTTG CTGGCTCAAA GCATCACCGT 
GCGGTGTCAT GGCGAGCTGT GTCCTGGGGC CTTGGGCTGC AGTTTGTGCT TGGGCTCTTC 
GTCATCAGAA CAGAACCAGG GTTCATTGCA TTCCAGTGGC TAGGGGATCA GATCCAGGTC 
TTCCTGAGTT ACACCGAGGC AGGCTCCAGC TTCGTCTTCG GAGAGGCTCT GGTGAAGGAT 
GTCTTTGCCT TTCAGGTTTT GCCCATCATC ATCTTCTTCA GCTGCGTCAT GTCTGTTCTG 
TACTATCTGG GCCTCATGCA GTGGGTGATC CTGAAGATTG CCTGGTTGAT GCAGGTCACC 
ATGGGCACCT CAGCCACCGA GACACTGAGT GTGGCGGGAA ACATCTTTGT GAGCCAGACT 
GAAGCTCCTC TGCTGATCCG GCCCTATCTG GCAGACATGA CACTCTCTGA AGTTCACGTT 
GTCATGACTG GAGGCTATGC TACCATTGCT GGCAGCCTCC TGGGCGCCTA CATCTCCTTT 
GGGATCGACG CTGCTTCCTT AATCGCAGCC TCTGTCATGG CCGCCCCTTG TGCGTTGGCT 
CTCTCCAAGC TGGTCTACCC AGAGGTGGAG GAGTCCAAGT TCCGGAGTGA GAATGGCGTG 
AAGCTGACCT ATGGAGACGC TCAGAACCTC TTGGAAGCAG CCAGTGCTGG GGCTGCCATC 
TCAGTGAAGG TCGTTGCCAA CATTGCTGCC AATCTGATTG CCTTCCTGGC TGTACTAGCC 
TTCGTCAATG CTGCCCTCTC CTGGCTAGGG GACATGGTGG ACATCCAGGG ACTCAGCTTC 
CAGCTCATCT GCTCCTACGT CCTGCGGCCT GTGGCCTTCT TGATGGGTGT GGCCTGGGAG 
GACTGTCCGG TAGTGGCTGA GTTGCTGGGC ATCAAGTTCT TTCTGAATGA GTTTGTGGCC 
TATCAAGAGC TTTCCCAGTA CAAGCAACGA CGCCTGGCAG GGGCTGAGGA GTGGCTTGGT 
GACAAGAAAC AGTGGATCTC TGTCAGAGCA GAAATCCTGA CTACATACGC CCTCTGTGGA 
TTTGCCAACT TCAGCTCCAT TGGCATCATG TTGGGAGGCC TGACCTCCCT AGTCCCCCAG 
CGGAGGAGCG ACTTCTCCCA GATTGTACTC CGGGCACTGA TCACAGGGGC TTTCGTGTCC 
CTGCTAAACG CCTGTGTGGC AGGGATCCTC TATGTACCCA GGGGGGTCGA GATGGACTGC 
GTGTCCCTTC TGAACCAAAC TGTCAGCAGC AGCAGCTTTG AGGTTTACCT GTGCTGCCGC 
CAAGTCTTCC AGAGCACTAG CTCGGAGTTC AGCCAAGTGG CACTGGACAA CTGCTGTCGA 
TTTTACAACC ACACAGTCTG CACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229485.1
CDS	877..2823
Translation	MADNTQRQRESISLTPMAHGLENMGAEFLESMEEGRLPHSHSSLPEGEGGLNKAERKAFSRWRSLQPTVQARSFCREHRQLFGWICKGLLSTACLGFLMVACLLDLQRALALLIITCVVLVFLAYDLLKRLLGSKLRRCVKFQGHSCLSLWLKRGLALAAGVGLILWLSLDTAQRPEQLVSFAGICVFLVLLFAGSKHHRAVSWRAVSWGLGLQFVLGLFVIRTEPGFIAFQWLGDQIQVFLSYTEAGSSFVFGEALVKDVFAFQVLPIIIFFSCVMSVLYYLGLMQWVILKIAWLMQVTMGTSATETLSVAGNIFVSQTEAPLLIRPYLADMTLSEVHVVMTGGYATIAGSLLGAYISFGIDAASLIAASVMAAPCALALSKLVYPEVEESKFRSENGVKLTYGDAQNLLEAASAGAAISVKVVANIAANLIAFLAVLAFVNAALSWLGDMVDIQGLSFQLICSYVLRPVAFLMGVAWEDCPVVAELLGIKFFLNEFVAYQELSQYKQRRLAGAEEWLGDKKQWISVRAEILTTYALCGFANFSSIGIMLGGLTSLVPQRRSDFSQIVLRALITGAFVSLLNACVAGILYVPRGVEMDCVSLLNQTVSSSSFEVYLCCRQVFQSTSSEFSQVALDNCCRFYNHTVCT

Target ORF information:

RefSeq Version	XM_006229423.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 28 member 1 (Slc28a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229423.3

ORF Insert Sequence:

ATGGCAGACA ACACACAGAG GCAAAGAGAG TCCATTTCCC TCACGCCTAT GGCCCACGGC 
CTGGAGAACA TGGGGGCAGA ATTCCTGGAA AGCATGGAGG AAGGCCGACT CCCTCACAGT 
CACTCAAGCC TGCCGGAGGG TGAAGGTGGC CTGAACAAAG CAGAGCGGAA GGCCTTCTCC 
CGATGGAGGA GTCTGCAGCC GACTGTGCAA GCGAGAAGCT TCTGCAGGGA GCACCGGCAG 
CTGTTTGGAT GGATCTGCAA AGGCCTGCTC TCTACTGCAT GTCTTGGCTT CTTGATGGTC 
GCCTGCCTCC TGGACCTCCA GAGGGCCCTA GCACTGTTGA TCATCACCTG TGTGGTTCTC 
GTCTTTCTGG CCTATGATCT GCTAAAGAGG CTTCTGGGGT CCAAGCTGAG GAGGTGTGTG 
AAGTTTCAAG GCCATTCTTG CCTGAGCCTC TGGCTGAAAA GAGGTCTAGC CCTTGCTGCT 
GGTGTGGGCC TGATCTTGTG GCTATCTCTG GACACCGCCC AGCGGCCTGA ACAGCTGGTG 
TCCTTTGCAG GGATCTGTGT GTTCCTTGTC CTTCTCTTTG CTGGCTCAAA GCATCACCGT 
GCGGTGTCAT GGCGAGCTGT GTCCTGGGGC CTTGGGCTGC AGTTTGTGCT TGGGCTCTTC 
GTCATCAGAA CAGAACCAGG GTTCATTGCA TTCCAGTGGC TAGGGGATCA GATCCAGGTC 
TTCCTGAGTT ACACCGAGGC AGGCTCCAGC TTCGTCTTCG GAGAGGCTCT GGTGAAGGAT 
GTCTTTGCCT TTCAGGTTTT GCCCATCATC ATCTTCTTCA GCTGCGTCAT GTCTGTTCTG 
TACTATCTGG GCCTCATGCA GTGGGTGATC CTGAAGATTG CCTGGTTGAT GCAGGTCACC 
ATGGGCACCT CAGCCACCGA GACACTGAGT GTGGCGGGAA ACATCTTTGT GAGCCAGACT 
GAAGCTCCTC TGCTGATCCG GCCCTATCTG GCAGACATGA CACTCTCTGA AGTTCACGTT 
GTCATGACTG GAGGCTATGC TACCATTGCT GGCAGCCTCC TGGGCGCCTA CATCTCCTTT 
GGGATCGACG CTGCTTCCTT AATCGCAGCC TCTGTCATGG CCGCCCCTTG TGCGTTGGCT 
CTCTCCAAGC TGGTCTACCC AGAGGTGGAG GAGTCCAAGT TCCGGAGTGA GAATGGCGTG 
AAGCTGACCT ATGGAGACGC TCAGAACCTC TTGGAAGCAG CCAGTGCTGG GGCTGCCATC 
TCAGTGAAGG TCGTTGCCAA CATTGCTGCC AATCTGATTG CCTTCCTGGC TGTACTAGCC 
TTCGTCAATG CTGCCCTCTC CTGGCTAGGG GACATGGTGG ACATCCAGGG ACTCAGCTTC 
CAGCTCATCT GCTCCTACGT CCTGCGGCCT GTGGCCTTCT TGATGGGTGT GGCCTGGGAG 
GACTGTCCGG TAGTGGCTGA GTTGCTGGGC ATCAAGTTCT TTCTGAATGA GTTTGTGGCC 
TATCAAGAGC TTTCCCAGTA CAAGCAACGA CGCCTGGCAG GGGCTGAGGA GTGGCTTGGT 
GACAAGAAAC AGTGGATCTC TGTCAGAGCA GAAATCCTGA CTACATACGC CCTCTGTGGA 
TTTGCCAACT TCAGCTCCAT TGGCATCATG TTGGGAGGCC TGACCTCCCT AGTCCCCCAG 
CGGAGGAGCG ACTTCTCCCA GATTGTACTC CGGGCACTGA TCACAGGGGC TTTCGTGTCC 
CTGCTAAACG CCTGTGTGGC AGGGATCCTC TATGTACCCA GGGGGGTCGA GATGGACTGC 
GTGTCCCTTC TGAACCAAAC TGTCAGCAGC AGCAGCTTTG AGGTTTACCT GTGCTGCCGC 
CAAGTCTTCC AGAGCACTAG CTCGGAGTTC AGCCAAGTGG CACTGGACAA CTGCTGTCGA 
TTTTACAACC ACACAGTCTG CACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc28a1 ( XM_006229422.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229484.1 Rattus norvegicus solute carrier family 28 member 1 (Slc28a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22931
Clone ID Related Accession (Same CDS sequence)	XM_006229423.3 , XM_006229422.3
Accession Version	XM_006229422.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium/nucleoside cotransporter 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229422.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACA ACACACAGAG GCAAAGAGAG TCCATTTCCC TCACGCCTAT GGCCCACGGC 
CTGGAGAACA TGGGGGCAGA ATTCCTGGAA AGCATGGAGG AAGGCCGACT CCCTCACAGT 
CACTCAAGCC TGCCGGAGGG TGAAGGTGGC CTGAACAAAG CAGAGCGGAA GGCCTTCTCC 
CGATGGAGGA GTCTGCAGCC GACTGTGCAA GCGAGAAGCT TCTGCAGGGA GCACCGGCAG 
CTGTTTGGAT GGATCTGCAA AGGCCTGCTC TCTACTGCAT GTCTTGGCTT CTTGATGGTC 
GCCTGCCTCC TGGACCTCCA GAGGGCCCTA GCACTGTTGA TCATCACCTG TGTGGTTCTC 
GTCTTTCTGG CCTATGATCT GCTAAAGAGG CTTCTGGGGT CCAAGCTGAG GAGGTGTGTG 
AAGTTTCAAG GCCATTCTTG CCTGAGCCTC TGGCTGAAAA GAGGTCTAGC CCTTGCTGCT 
GGTGTGGGCC TGATCTTGTG GCTATCTCTG GACACCGCCC AGCGGCCTGA ACAGCTGGTG 
TCCTTTGCAG GGATCTGTGT GTTCCTTGTC CTTCTCTTTG CTGGCTCAAA GCATCACCGT 
GCGGTGTCAT GGCGAGCTGT GTCCTGGGGC CTTGGGCTGC AGTTTGTGCT TGGGCTCTTC 
GTCATCAGAA CAGAACCAGG GTTCATTGCA TTCCAGTGGC TAGGGGATCA GATCCAGGTC 
TTCCTGAGTT ACACCGAGGC AGGCTCCAGC TTCGTCTTCG GAGAGGCTCT GGTGAAGGAT 
GTCTTTGCCT TTCAGGTTTT GCCCATCATC ATCTTCTTCA GCTGCGTCAT GTCTGTTCTG 
TACTATCTGG GCCTCATGCA GTGGGTGATC CTGAAGATTG CCTGGTTGAT GCAGGTCACC 
ATGGGCACCT CAGCCACCGA GACACTGAGT GTGGCGGGAA ACATCTTTGT GAGCCAGACT 
GAAGCTCCTC TGCTGATCCG GCCCTATCTG GCAGACATGA CACTCTCTGA AGTTCACGTT 
GTCATGACTG GAGGCTATGC TACCATTGCT GGCAGCCTCC TGGGCGCCTA CATCTCCTTT 
GGGATCGACG CTGCTTCCTT AATCGCAGCC TCTGTCATGG CCGCCCCTTG TGCGTTGGCT 
CTCTCCAAGC TGGTCTACCC AGAGGTGGAG GAGTCCAAGT TCCGGAGTGA GAATGGCGTG 
AAGCTGACCT ATGGAGACGC TCAGAACCTC TTGGAAGCAG CCAGTGCTGG GGCTGCCATC 
TCAGTGAAGG TCGTTGCCAA CATTGCTGCC AATCTGATTG CCTTCCTGGC TGTACTAGCC 
TTCGTCAATG CTGCCCTCTC CTGGCTAGGG GACATGGTGG ACATCCAGGG ACTCAGCTTC 
CAGCTCATCT GCTCCTACGT CCTGCGGCCT GTGGCCTTCT TGATGGGTGT GGCCTGGGAG 
GACTGTCCGG TAGTGGCTGA GTTGCTGGGC ATCAAGTTCT TTCTGAATGA GTTTGTGGCC 
TATCAAGAGC TTTCCCAGTA CAAGCAACGA CGCCTGGCAG GGGCTGAGGA GTGGCTTGGT 
GACAAGAAAC AGTGGATCTC TGTCAGAGCA GAAATCCTGA CTACATACGC CCTCTGTGGA 
TTTGCCAACT TCAGCTCCAT TGGCATCATG TTGGGAGGCC TGACCTCCCT AGTCCCCCAG 
CGGAGGAGCG ACTTCTCCCA GATTGTACTC CGGGCACTGA TCACAGGGGC TTTCGTGTCC 
CTGCTAAACG CCTGTGTGGC AGGGATCCTC TATGTACCCA GGGGGGTCGA GATGGACTGC 
GTGTCCCTTC TGAACCAAAC TGTCAGCAGC AGCAGCTTTG AGGTTTACCT GTGCTGCCGC 
CAAGTCTTCC AGAGCACTAG CTCGGAGTTC AGCCAAGTGG CACTGGACAA CTGCTGTCGA 
TTTTACAACC ACACAGTCTG CACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229484.1
CDS	764..2710
Translation	MADNTQRQRESISLTPMAHGLENMGAEFLESMEEGRLPHSHSSLPEGEGGLNKAERKAFSRWRSLQPTVQARSFCREHRQLFGWICKGLLSTACLGFLMVACLLDLQRALALLIITCVVLVFLAYDLLKRLLGSKLRRCVKFQGHSCLSLWLKRGLALAAGVGLILWLSLDTAQRPEQLVSFAGICVFLVLLFAGSKHHRAVSWRAVSWGLGLQFVLGLFVIRTEPGFIAFQWLGDQIQVFLSYTEAGSSFVFGEALVKDVFAFQVLPIIIFFSCVMSVLYYLGLMQWVILKIAWLMQVTMGTSATETLSVAGNIFVSQTEAPLLIRPYLADMTLSEVHVVMTGGYATIAGSLLGAYISFGIDAASLIAASVMAAPCALALSKLVYPEVEESKFRSENGVKLTYGDAQNLLEAASAGAAISVKVVANIAANLIAFLAVLAFVNAALSWLGDMVDIQGLSFQLICSYVLRPVAFLMGVAWEDCPVVAELLGIKFFLNEFVAYQELSQYKQRRLAGAEEWLGDKKQWISVRAEILTTYALCGFANFSSIGIMLGGLTSLVPQRRSDFSQIVLRALITGAFVSLLNACVAGILYVPRGVEMDCVSLLNQTVSSSSFEVYLCCRQVFQSTSSEFSQVALDNCCRFYNHTVCT

Target ORF information:

RefSeq Version	XM_006229422.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 28 member 1 (Slc28a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229422.3

ORF Insert Sequence:

ATGGCAGACA ACACACAGAG GCAAAGAGAG TCCATTTCCC TCACGCCTAT GGCCCACGGC 
CTGGAGAACA TGGGGGCAGA ATTCCTGGAA AGCATGGAGG AAGGCCGACT CCCTCACAGT 
CACTCAAGCC TGCCGGAGGG TGAAGGTGGC CTGAACAAAG CAGAGCGGAA GGCCTTCTCC 
CGATGGAGGA GTCTGCAGCC GACTGTGCAA GCGAGAAGCT TCTGCAGGGA GCACCGGCAG 
CTGTTTGGAT GGATCTGCAA AGGCCTGCTC TCTACTGCAT GTCTTGGCTT CTTGATGGTC 
GCCTGCCTCC TGGACCTCCA GAGGGCCCTA GCACTGTTGA TCATCACCTG TGTGGTTCTC 
GTCTTTCTGG CCTATGATCT GCTAAAGAGG CTTCTGGGGT CCAAGCTGAG GAGGTGTGTG 
AAGTTTCAAG GCCATTCTTG CCTGAGCCTC TGGCTGAAAA GAGGTCTAGC CCTTGCTGCT 
GGTGTGGGCC TGATCTTGTG GCTATCTCTG GACACCGCCC AGCGGCCTGA ACAGCTGGTG 
TCCTTTGCAG GGATCTGTGT GTTCCTTGTC CTTCTCTTTG CTGGCTCAAA GCATCACCGT 
GCGGTGTCAT GGCGAGCTGT GTCCTGGGGC CTTGGGCTGC AGTTTGTGCT TGGGCTCTTC 
GTCATCAGAA CAGAACCAGG GTTCATTGCA TTCCAGTGGC TAGGGGATCA GATCCAGGTC 
TTCCTGAGTT ACACCGAGGC AGGCTCCAGC TTCGTCTTCG GAGAGGCTCT GGTGAAGGAT 
GTCTTTGCCT TTCAGGTTTT GCCCATCATC ATCTTCTTCA GCTGCGTCAT GTCTGTTCTG 
TACTATCTGG GCCTCATGCA GTGGGTGATC CTGAAGATTG CCTGGTTGAT GCAGGTCACC 
ATGGGCACCT CAGCCACCGA GACACTGAGT GTGGCGGGAA ACATCTTTGT GAGCCAGACT 
GAAGCTCCTC TGCTGATCCG GCCCTATCTG GCAGACATGA CACTCTCTGA AGTTCACGTT 
GTCATGACTG GAGGCTATGC TACCATTGCT GGCAGCCTCC TGGGCGCCTA CATCTCCTTT 
GGGATCGACG CTGCTTCCTT AATCGCAGCC TCTGTCATGG CCGCCCCTTG TGCGTTGGCT 
CTCTCCAAGC TGGTCTACCC AGAGGTGGAG GAGTCCAAGT TCCGGAGTGA GAATGGCGTG 
AAGCTGACCT ATGGAGACGC TCAGAACCTC TTGGAAGCAG CCAGTGCTGG GGCTGCCATC 
TCAGTGAAGG TCGTTGCCAA CATTGCTGCC AATCTGATTG CCTTCCTGGC TGTACTAGCC 
TTCGTCAATG CTGCCCTCTC CTGGCTAGGG GACATGGTGG ACATCCAGGG ACTCAGCTTC 
CAGCTCATCT GCTCCTACGT CCTGCGGCCT GTGGCCTTCT TGATGGGTGT GGCCTGGGAG 
GACTGTCCGG TAGTGGCTGA GTTGCTGGGC ATCAAGTTCT TTCTGAATGA GTTTGTGGCC 
TATCAAGAGC TTTCCCAGTA CAAGCAACGA CGCCTGGCAG GGGCTGAGGA GTGGCTTGGT 
GACAAGAAAC AGTGGATCTC TGTCAGAGCA GAAATCCTGA CTACATACGC CCTCTGTGGA 
TTTGCCAACT TCAGCTCCAT TGGCATCATG TTGGGAGGCC TGACCTCCCT AGTCCCCCAG 
CGGAGGAGCG ACTTCTCCCA GATTGTACTC CGGGCACTGA TCACAGGGGC TTTCGTGTCC 
CTGCTAAACG CCTGTGTGGC AGGGATCCTC TATGTACCCA GGGGGGTCGA GATGGACTGC 
GTGTCCCTTC TGAACCAAAC TGTCAGCAGC AGCAGCTTTG AGGTTTACCT GTGCTGCCGC 
CAAGTCTTCC AGAGCACTAG CTCGGAGTTC AGCCAAGTGG CACTGGACAA CTGCTGTCGA 
TTTTACAACC ACACAGTCTG CACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of concentrative nucleoside transporters SLC28 (CNT1, CNT2, and CNT3) along the rat nephron: effect of diabetes.
Kidney international68(2)665-72(2005 Aug)
Rodr?guez-Mulero S,Errasti-Murugarren E,Ballar?n J,Felipe A,Doucet A,Casado FJ,Pastor-Anglada M

Concentrative nucleoside transporter (rCNT1) is targeted to the apical membrane through the hepatic transcytotic pathway.
Experimental cell research281(1)77-85(2002 Nov)
Duflot S,Calvo M,Casado FJ,Enrich C,Pastor-Anglada M

Cell-cycle-dependent regulation of CNT1, a concentrative nucleoside transporter involved in the uptake of cell-cycle-dependent nucleoside-derived anticancer drugs.
Biochemical and biophysical research communications296(3)575-9(2002 Aug)
Vald?s R,Casado FJ,Pastor-Anglada M

Na+-dependent nucleoside transport in liver: two different isoforms from the same gene family are expressed in liver cells.
The Biochemical journal330 ( Pt 2)997-1001(1998 Mar)
Felipe A,Valdes R,Santo B,Lloberas J,Casado J,Pastor-Anglada M

Stable expression of a recombinant sodium-dependent, pyrimidine-selective nucleoside transporter (CNT1) in a transport-deficient mouse leukemia cell line.
Biochemistry and cell biology = Biochimie et biologie cellulaire76(5)843-51(1998)
Crawford CR,Cass CE,Young JD,Belt JA

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Slc28a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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