Tnc cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tnc
Entrez Gene ID	116640
Full Name	tenascin C
General protein information	Preferred Names tenascin C Names tenascin Tn-C AD1 tenascin-C AD1 domain
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q24
Summary	extracellular matrix glycoprotein; inhibits the attachment of fibroblasts to adhesive proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008763754.2	XP_008761976.1	tenascin isoform X1
XM_008763755.2	XP_008761977.1	tenascin isoform X2
NM_053861.1	NP_446313.1	tenascin precursor
XM_008763756.2	XP_008761978.1	tenascin isoform X3
XM_008763757.2	XP_008761979.1	tenascin isoform X4
XM_008763758.2	XP_008761980.1	tenascin isoform X5

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tnc	NP_035737.2
Gallus gallus (chicken)	TNC	NP_990787.2
Xenopus tropicalis (tropical clawed frog)	tnc	XP_004916768.1
Pan troglodytes (chimpanzee)	TNC	XP_001156720.1
Danio rerio (zebrafish)	LOC100149028	XP_005171797.1
Canis lupus familiaris (dog)	TNC	NP_001182078.1
Rattus norvegicus (Norway rat)	Tnc	NP_446313.1
Homo sapiens (human)	TNC	NP_002151.2

Gene Ontology
Bibliography

Related articles in PubMed

CD73-derived adenosine and tenascin-C control cytokine production by epicardium-derived cells formed after myocardial infarction.
Hesse J, Leberling S, Boden E, Friebe D, Schmidt T, Ding Z, Dieterich P, Deussen A, Roderigo C, Rose CR, Floss DM, Scheller J, Schrader J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(7)3040-3053(2017 Jul)

Tenascin-C expression and its associated pathway in BMSCs following co-culture with mechanically stretched ligament fibroblasts.
Zhao B, Hu M, Wu H, Ren C, Wang J, Cui S
Molecular medicine reports15(5)2465-2472(2017 May)

Early remodelling of the extracellular matrix proteins tenascin-C and phosphacan in retina and optic nerve of an experimental autoimmune glaucoma model.
Reinehr S, Reinhard J, Wiemann S, Stute G, Kuehn S, Woestmann J, Dick HB, Faissner A, Joachim SC
Journal of cellular and molecular medicine20(11)2122-2137(2016 11)

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Epicardium-derived cells (EPDCs) from post-myocardial infarction hearts highly expressed tenascin-C, which induces pro-IL-1beta transcription in EPDCs and thus contributes to inflammasome activation.
Title: CD73-derived adenosine and tenascin-C control cytokine production by epicardium-derived cells formed after myocardial infarction.

tenascin-C and phosphacan represent early indicators of neurodegeneration.
Title: Early remodelling of the extracellular matrix proteins tenascin-C and phosphacan in retina and optic nerve of an experimental autoimmune glaucoma model.

In a liver fibrosis cellular model, tenascin-C promotes migration of hepatic stellate cells and production of type I collagen via TGF-beta1 and alpha9beta1 integrin pathway.
Title: Tenascin-C promotes migration of hepatic stellate cells and production of type I collagen.

Co-localization of tenascin-C and fibronectin immunoreactivity was observed in many regions
Title: Immunohistochemical localization of tenascin-C in rat periodontal ligament with reference to alveolar bone remodeling.

the D/P Cr ratio was correlated with effluent levels of TN-C (rho = 0.57, p < 0.001) and MMP-2 (rho = 0.73, p < 0.001). We suggest that TN-C in the effluents may be a diagnostic marker for peritoneal deterioration with EMT-like change
Title: Effluent Tenascin-C Levels Reflect Peritoneal Deterioration in Peritoneal Dialysis: MAJOR IN PD Study.

The following Tnc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tnc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa42078	NM_053861.1 Latest version!	Rattus norvegicus tenascin C (Tnc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ORa44020	XM_008763754.2 Latest version!	Rattus norvegicus tenascin C (Tnc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
ORa44021	XM_008763755.2 Latest version!	Rattus norvegicus tenascin C (Tnc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
ORa44022	XM_008763756.2 Latest version!	Rattus norvegicus tenascin C (Tnc), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
ORa44023	XM_008763757.2 Latest version!	Rattus norvegicus tenascin C (Tnc), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
ORa44024	XM_008763758.2 Latest version!	Rattus norvegicus tenascin C (Tnc), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tnc ( NM_053861.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446313.1 Rattus norvegicus tenascin C (Tnc), mRNA.

Price & Availability↑

CloneID	ORa42078
Clone ID Related Accession (Same CDS sequence)	NM_053861.1
Accession Version	NM_053861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	tenascin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU596506.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU596506.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGGGCCG TGRCCTGGCT ATTGCCAGGC ATCTTCCTAG CTTTGTTTGC CCTCACTCCT 
GAAGGTGGGG TCCTCAAGAA AGTCATCAGG CACAAGCGAG AGAGTGGGCT GAACTTGACC 
TTGCCAGAGG AGAATCAGCC AGTGGTGTTC AACCATGTCT ACAACATCAA GTTGCCCATG 
GGTTCTCAGT GCTCAGTGGA TCTAGAGTCA ACGAGTGGAG AGAAAGACCT GAGCCCCACA 
CCAGAGTCCA GTGGAAGCTT CCAGGAGCAT ACAGTGGATG GGGAAAATCA GATTGTGTTC 
ACACACCGCA TCAACATCCC TCGTCGGGCC TGTGGCTGTG CCGCAGCTCC AGATGTGAAG 
GAGCTCCTGA GCAGACTGGA GGAACTGGAG ATGTTGGTAT CTTCTCTAAG GGAGCAGTGC 
ACCATGGGTA CAGGCTGTTG CCTCCAACCT GCAGAAGGCC GTCTGGACAG CAGGCCCTTC 
TGCAGCGGCA GGGGTAACTT CAGTGCTGAA GGTTGTGGCT GTGTCTGTGA ACCAGGCTGG 
AAAGGTCCCA ACTGCTCTGA GCCTGAATGC CCTGGAAACT GTAATCTCAG AGGCCAGTGC 
CTTGATGGAC AGTGTATCTG TGACCAGGGT TTCACTGGGG AAGACTGCAG CCAGCTAGCC 
TGTCCCAATG ACTGCAATGA CCAGGGCAAG TGTGTGAATG GGGTCTGTGT GTGCTTCGAA 
GGCTATGCCG GCCTTGACTG TGGCCTGGAA GTCTGCCCAG TGCCGTGCAG CGAGGAACAC 
GGGATGTGTG TGGATGGCAG GTGTGTGTGC AAAGATGGCT TTGCTGGTGA AGACTGCAAT 
GAGCCCCTTT GCTTCAACAA CTGCAACAAC CGTGGGCGGT GTGTGGAGAA CGAATGCGTC 
TGTGATGAGG GCTTCACGGG CGAAGACTGC AGCGAGCTCA TTTGCCCCAA TGACTGCTTC 
GACCGAGGTC GCTGCATCAA CGGCACCTGC TACTGTGAAG AAGGTTTCAC AGGTGAAGAC 
TGTGGTGAGC TCACCTGCCC CAACAACTGT CAGGGCCATG GCCAGTGTGA AGAGGGACAG 
TGTGTTTGCA ACGAGGGCTT TGCAGGGGCA GACTGCAGTG AAAAGCGGTG TCCCGCAGAC 
TGTCACCACC ATGGCCGCTG TCTCAACGGA CAGTGTGAGT GTGACGATGG GTTCACAGGG 
GCTGACTGTG GGGAACTCCA GTGCCCCAAT GGCTGCAGTG GGCATGGCCG CTGTGTCAAT 
GGGCAGTGTG TGTGTGACGA GGGCTACACC GGAGAAGACT GTAGCCAACG GCGATGCCCC 
AATGACTGCC ACAACCGGGG TCACTGTGTA CAGGGCAAAT GCATATGTGA GCAAGGCTTC 
AAAGGCTTTG ACTGTAGTGA GATGAGCTGT CCCAATGACT GCCACCAGCA TGGCCGCTGT 
GTGAATGGCA TGTGCATCTG TGATGATGAC TACACTGGGG AAGACTGCAG AGACCGCCGC 
TGTCCCCGGG ACTGTAGCCA GCGGGGGCGC TGTGTGGACG GACAGTGCAT TTGTGAGGAT 
GGTTTCACTG GTTCTGACTG TGCTGAGCTC TCCTGCCCTG GTGACTGCCA CGGCCATGGC 
CGCTGTGTGA ATGGCCAATG CATCTGCCAC GAGGGCTTCA CCGGCRAAGA CTGCAAAGAG 
CAAAGGTGCC CCAGTGACTG CCATGGCCAA GGCCGCTGTG AGGACGGCCA ATGTATCTGC 
CATGAGGGCT TCACAGGCCT GGACTGTGGG CAGCGCTCCT GTCCCAATGA CTGCAGCAAC 
CAGGGACAAT GTGTGGCAGG CCGCTGCATC TGCAATGAAG GCTACACAGG GGTAGACTGC 
TCTGAGGTGT CCCCTCCCAA AGACCTTATT GTGACAGAAG TAACAGAGGA GACTGTARAT 
CTGGCATGGG ACAATGAGAT GCGGGTCACT GAGTACCTCA TTATGTACAC ACCCACCCAT 
GCTGATGGCC TGGAGATGCA GTTCCGTGTG CCTGGGGACC AGACATCTAC CACCATCCGG 
GAGCTGGAGC CAGGAGTGGA GTACTTCATT CGTGTGTTCG CCATCTTGGA GAACAAGAGG 
AGCATCCCTG TCAGTGCCAG AGTTGCCACC TACTTGCCTG CACCTGAAGG CCTAAAATTC 
AAGTCTATCA AGGAGACATC TGTGGAAGTA GAGTGGGATC CTCTGGACAT CGCTTTTGAA 
ACGTGGGAGA TCATTTTCAG AAATATGAAC AAAGAAGATG AGGGAGAGAT CACAAAAAGC 
TTGAGGAGGC CAGAGACCTC CTACCGCCAA ACTGGCCTGG CTCCTGGCCA AGAATATGAA 
ATATCTCTTC ACATTGTGAA AAACAACACC CGAGGCCCTG GCTTGAAGAA AGTGACCACA 
ACTCGCCTGG ATGCCCCCAG CCAAATTGAG GTGAGAGACA TCACAGACAC TACAGCACTG 
ATCACCTGGT CCAAGCCCTT GGCTGAAATT GATAGCATTG AGCTCTCCTA TGGCATCAAG 
GATGTGCCTG GAGACCGGAC CACCATCGAC CTCACACATG AAGACAACCA GTACTCCATC 
GGGAACCTGA AACCTGACAC GGAGTATGAG GTGTCCCTCA TCTCCCGCAG AGTGGACATG 
GCAAGCAACC CTGCCAAGGA GACCTTCATC ACAGGCCTGG ATGCTCCCAG GAATCTCCGT 
CGCGTCTCAC AGACAGACAA CAGCATCACC TTGGAGTGGA GGAATGTCAA GGCAGACATT 
GATAGTTATA GAATTAAGTA TGCACCTATC TCTGGAGGTG ACCATGCTGA GATAGATGTT 
CCAAAGAGCC AGCAAGCCAC AACCAAAACC ACACTCACAG GTCTAAGGCC AGGAACTGAA 
TATGGAGTGG GCGTCTCTGC TGTTAAGGGA GACAAGGAGG GCGATCCAGC AACCATCAAT 
GCAGCCACAG AAATTGATGC ACCCAGGGAC TTACAGGTGT CTGAGACCAC ACAAGACAGT 
CTGACTTTAT TCTGGAAGAC ACCTCTGGCC AAGTTTGATC GTTACCGCCT CAACTACAGC 
CTCCCCACAG GCCAGTCAAT AGAGATTCAG CTGCCAAAGG ATGCCACCTC CCATGTCCTG 
ACAGACCTGG AGCCAGGGCA AGAATACACT GTTCTCCTCA CCGCTGAGAA GGGCAGACAT 
AAGAGCAAGC CTGCGCGTGT GAAGGCATCC ACAGAAGAAG TACCTTCTCT GGAAAATCTC 
ACCGTGACTG AGGCCGGCTG GGATGGCCTC AGACTCAACT GGACTGCAGA TGACCTGGCC 
TATGAGTACT TTGTTATTCA GGTACAGGAA GCCAACAAGG TGGAGACTGC TCACAACTTC 
ACAGTACTTG GTAACCTCCG GACTGCAGAC ATCCCGGGCC TCAAGGCTGC CACTCCTTAT 
AGAGTCTCCA TTTATGGGGT AGCTCGGGGC TATAAAACAC CCGTGCTCTC TGCTGAGACC 
TTCACAGGGA AAACTCCCAG TTTGGGAGAG GTGACTGTGG CTGAGGTGGG CTGGGATGCC 
CTCAAGCTCA ACTGGACTGC TCCAGAAGGA GCCTATACGA ACTTTTTCAT TCAGGTGCTA 
GAGGCTGACA TGACCCAGAC TGTCCAGAAC CTCACAGTCC CAGGGGGACT GAGGTCAGTG 
GACCTACCTG GGCTCAAAGC AGCCACCCGC TATTACATCA CCGTTCGAGG GGTCACCCAG 
GACTTCAGCA CAGCCCCTCT CTCTGTTGAG GTCTTGACAG AGGAGATCCC TCAACTGGGA 
GGCTTGTCCG TGACTGAGGT CAGCTGGGAC AGCCTTACAC TCAACTGGAC CACAGATGAT 
CTGGCCTATA AGCACTTTAT CATTCAGGTG CAGGAGGCCA ACAATGTAGA GGCTGCTCAG 
AACCTCACAG TATCTGGTAG CCTCAGAGTT GTGGACATCC CAGGCCTCAA GGCTGATACC 
CCTTATAGAG TCTCCATCTA TGGGGTGATC CAGGGCTATA GAACACCAAT GCTCTCTGCT 
GATGTCTCCA CAGCCAAAGA ACCTGAAATT GGAAACTTAA ATATTTCTGA TGTAACTCCT 
GAGAGCTTCA ATCTCTCCTG GACAGCTACC GACGGGATCT TCGACATGTT TACTATCGAA 
ATTATTGATT CTAATAGGTT GCTGCAGACA GCAGAACATA ATATATCTGG TGCTGAACGA 
ACTGCCCACA TCTCGGGCCT TCCACCTAGT ACTGATTTCA TTGTCTACCT CTCTGGAATT 
GCTCCCAGCA TCCGTACCAA AACCATCAGT ACCACAGCCA CCACAGAAGC TGAACCGGAA 
GTTGACAACC TTCTAGTTTC AGATGCTACT CCAGACGGTT TCTGTCTGTC CTGGACTGCT 
GATGAAGGGA TATTCGACAG TTTTGTTATC AGGATCAGAG ATACCAAAAA GCAATCTGAA 
CCACAAGAAA TAACCCTCCC TTCCCCCGAC CGTACCAGGG ACATAACAGG TCTCAGAGAG 
GCCACTGAGT ACGAAATTGA ACTCTATGGA ATAAGCCGTG GAAGGCGATC CCAGCCAGTC 
AGTGCCATAG CAACAACAGC CATGGGCTCT CCGAAGGAAA TCATGTTCTC AGACATCACT 
GAAAATGCAG CCACAGTCAG CTGGAGGGCA CCGACTGCTC AGGTGGAGAG TTTCCGGATT 
ACTTATGTGC CTGTAACAGG AGGTCCCCCA TCCATGGTGA CCGTGGATGG GACGGATACT 
GAGACCCGAC TGGTGAGGCT CACCCCTGGA GTGGAGTACC ACGTCAGTGT GATTGCCATG 
AAGGGATTTG AAGAAAGTGA TCCGGTCTCG GGGTCTCTAA TCACAGCTCT CGATGGTCCA 
TCTGGCCTTC TGACAGCCAA CATCACAGAC TCAGAAGCCT TGGCCATGTG GCAGCCGGCC 
ATTGCCACTG TGGACAGTTA TGTCATCTCC TACACAGGGG AGAGAGTGCC AGAAATTACC 
CGCACAGTGT CTGGGAATAC AGTGGAGTTT GAACTACATG ACCTGGAGCC TGCCACAGAG 
TACACACTGA GTGTCTTTGC AGAGAAAGGT CACCAGAAGA GTTCTACCAT CGCTACGAAA 
TTTACCACAG ACCTGGATTC CCCAAGAGAA TTGACTGCTA CTGAGGTTCA GTCAGAAACT 
GCCTTCCTCA CCTGGAGACC TCCCCGGGCA TCGGTCACTG GATACCTCCT GGTCTATGAG 
TCTGTGGATG GTACAGTCAA GGAAGTCATT GTGGGGCCTG ACACCACCTC CTACAGCCTG 
GCAGACCTGA GCCCATCCAC CCACTACACA GCAAGAATCC AGGCATTGAG TGGGTCCCTG 
AGGAGCAAAC TGATCCAAAC CATCTTCACC ACAATTGGAC TCCTATATCC ATTCCCTAGG 
GATTGCTCTC AAGCAATGTT GAATGGCGAC ACCACCTCTG GCCTCTATAC CATCTATATA 
AATGGTGACA AGACTCAAGC ACTGGAAGTC TACTGTGATA TGACCTCTGA TGGAGGTGGA 
TGGATCGTTT TCCTGAGACG CAAAAATGGA CGTGAGGACT TCTATCGAAA CTGGAAGGCC 
TATGCCACTG GGTTTGGAGA CCGCAGAGAA GAATTCTGGC TTGGACTGGA TAACCTGAGC 
AAAATCACAG CCCAAGGGCA ATATGAGCTC CGGGTGGACC TACAAGACCA TGGGGAGTCA 
GCCTATGCTG TGTATGACAG GTTCAGTGTT GGAGATGCCA AGAGTCGCTA CAAGCTGAAG 
GTAGAAGGAT ACAGTGGAAC GGCAGGTGAC TCCATGAACT ATCACAATGG TAGATCCTTC 
TCTACCTATG ACAAGGACAC AGACTCAGCC ATCACCAACT GTGCCCTGTC CTACAAAGGA 
GCTTTCTGGT ATAAGAACTG TCATCGTGTC AACCTGATGG GCAGATATGG GGACAATAAT 
CACAGTCAGG GTGTTAACTG GTTCCACTGG AAGGGCCATG AGTACTCAAT CCAGTTTGCG 
GAGATGAAAC TAAGACCCAG CAACTTCCGA AATCTGGAAG GCAGGCGTAA GCGGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446313.1
CDS	42..6101
Translation	MGAVnullWLLPGIFLALFALTPEGGVLKKVIRHKRESGLNLTLPEENQPVVFNHVYNIKLPMGSQCSVDLESTSGEKDLSPTPESSGSFQEHTVDGENQIVFTHRINIPRRACGCAAAPDVKELLSRLEELEMLVSSLREQCTMGTGCCLQPAEGRLDSRPFCSGRGNFSAEGCGCVCEPGWKGPNCSEPECPGNCNLRGQCLDGQCICDQGFTGEDCSQLACPNDCNDQGKCVNGVCVCFEGYAGLDCGLEVCPVPCSEEHGMCVDGRCVCKDGFAGEDCNEPLCFNNCNNRGRCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGELTCPNNCQGHGQCEEGQCVCNEGFAGADCSEKRCPADCHHHGRCLNGQCECDDGFTGADCGELQCPNGCSGHGRCVNGQCVCDEGYTGEDCSQRRCPNDCHNRGHCVQGKCICEQGFKGFDCSEMSCPNDCHQHGRCVNGMCICDDDYTGEDCRDRRCPRDCSQRGRCVDGQCICEDGFTGSDCAELSCPGDCHGHGRCVNGQCICHEGFTGnullDCKEQRCPSDCHGQGRCEDGQCICHEGFTGLDCGQRSCPNDCSNQGQCVAGRCICNEGYTGVDCSEVSPPKDLIVTEVTEETVnullLAWDNEMRVTEYLIMYTPTHADGLEMQFRVPGDQTSTTIRELEPGVEYFIRVFAILENKRSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEGEITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKKVTTTRLDAPSQIEVRDITDTTALITWSKPLAEIDSIELSYGIKDVPGDRTTIDLTHEDNQYSIGNLKPDTEYEVSLISRRVDMASNPAKETFITGLDAPRNLRRVSQTDNSITLEWRNVKADIDSYRIKYAPISGGDHAEIDVPKSQQATTKTTLTGLRPGTEYGVGVSAVKGDKEGDPATINAATEIDAPRDLQVSETTQDSLTLFWKTPLAKFDRYRLNYSLPTGQSIEIQLPKDATSHVLTDLEPGQEYTVLLTAEKGRHKSKPARVKASTEEVPSLENLTVTEAGWDGLRLNWTADDLAYEYFVIQVQEANKVETAHNFTVLGNLRTADIPGLKAATPYRVSIYGVARGYKTPVLSAETFTGKTPSLGEVTVAEVGWDALKLNWTAPEGAYTNFFIQVLEADMTQTVQNLTVPGGLRSVDLPGLKAATRYYITVRGVTQDFSTAPLSVEVLTEEIPQLGGLSVTEVSWDSLTLNWTTDDLAYKHFIIQVQEANNVEAAQNLTVSGSLRVVDIPGLKADTPYRVSIYGVIQGYRTPMLSADVSTAKEPEIGNLNISDVTPESFNLSWTATDGIFDMFTIEIIDSNRLLQTAEHNISGAERTAHISGLPPSTDFIVYLSGIAPSIRTKTISTTATTEAEPEVDNLLVSDATPDGFCLSWTADEGIFDSFVIRIRDTKKQSEPQEITLPSPDRTRDITGLREATEYEIELYGISRGRRSQPVSAIATTAMGSPKEIMFSDITENAATVSWRAPTAQVESFRITYVPVTGGPPSMVTVDGTDTETRLVRLTPGVEYHVSVIAMKGFEESDPVSGSLITALDGPSGLLTANITDSEALAMWQPAIATVDSYVISYTGERVPEITRTVSGNTVEFELHDLEPATEYTLSVFAEKGHQKSSTIATKFTTDLDSPRELTATEVQSETAFLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTARIQALSGSLRSKLIQTIFTTIGLLYPFPRDCSQAMLNGDTTSGLYTIYINGDKTQALEVYCDMTSDGGGWIVFLRRKNGREDFYRNWKAYATGFGDRREEFWLGLDNLSKITAQGQYELRVDLQDHGESAYAVYDRFSVGDAKSRYKLKVEGYSGTAGDSMNYHNGRSFSTYDKDTDSAITNCALSYKGAFWYKNCHRVNLMGRYGDNNHSQGVNWFHWKGHEYSIQFAEMKLRPSNFRNLEGRRKRA

Target ORF information:

RefSeq Version	NM_053861.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tenascin C (Tnc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053861.1

ORF Insert Sequence:

ATGGGGGCCG TGRCCTGGCT ATTGCCAGGC ATCTTCCTAG CTTTGTTTGC CCTCACTCCT 
GAAGGTGGGG TCCTCAAGAA AGTCATCAGG CACAAGCGAG AGAGTGGGCT GAACTTGACC 
TTGCCAGAGG AGAATCAGCC AGTGGTGTTC AACCATGTCT ACAACATCAA GTTGCCCATG 
GGTTCTCAGT GCTCAGTGGA TCTAGAGTCA ACGAGTGGAG AGAAAGACCT GAGCCCCACA 
CCAGAGTCCA GTGGAAGCTT CCAGGAGCAT ACAGTGGATG GGGAAAATCA GATTGTGTTC 
ACACACCGCA TCAACATCCC TCGTCGGGCC TGTGGCTGTG CCGCAGCTCC AGATGTGAAG 
GAGCTCCTGA GCAGACTGGA GGAACTGGAG ATGTTGGTAT CTTCTCTAAG GGAGCAGTGC 
ACCATGGGTA CAGGCTGTTG CCTCCAACCT GCAGAAGGCC GTCTGGACAG CAGGCCCTTC 
TGCAGCGGCA GGGGTAACTT CAGTGCTGAA GGTTGTGGCT GTGTCTGTGA ACCAGGCTGG 
AAAGGTCCCA ACTGCTCTGA GCCTGAATGC CCTGGAAACT GTAATCTCAG AGGCCAGTGC 
CTTGATGGAC AGTGTATCTG TGACCAGGGT TTCACTGGGG AAGACTGCAG CCAGCTAGCC 
TGTCCCAATG ACTGCAATGA CCAGGGCAAG TGTGTGAATG GGGTCTGTGT GTGCTTCGAA 
GGCTATGCCG GCCTTGACTG TGGCCTGGAA GTCTGCCCAG TGCCGTGCAG CGAGGAACAC 
GGGATGTGTG TGGATGGCAG GTGTGTGTGC AAAGATGGCT TTGCTGGTGA AGACTGCAAT 
GAGCCCCTTT GCTTCAACAA CTGCAACAAC CGTGGGCGGT GTGTGGAGAA CGAATGCGTC 
TGTGATGAGG GCTTCACGGG CGAAGACTGC AGCGAGCTCA TTTGCCCCAA TGACTGCTTC 
GACCGAGGTC GCTGCATCAA CGGCACCTGC TACTGTGAAG AAGGTTTCAC AGGTGAAGAC 
TGTGGTGAGC TCACCTGCCC CAACAACTGT CAGGGCCATG GCCAGTGTGA AGAGGGACAG 
TGTGTTTGCA ACGAGGGCTT TGCAGGGGCA GACTGCAGTG AAAAGCGGTG TCCCGCAGAC 
TGTCACCACC ATGGCCGCTG TCTCAACGGA CAGTGTGAGT GTGACGATGG GTTCACAGGG 
GCTGACTGTG GGGAACTCCA GTGCCCCAAT GGCTGCAGTG GGCATGGCCG CTGTGTCAAT 
GGGCAGTGTG TGTGTGACGA GGGCTACACC GGAGAAGACT GTAGCCAACG GCGATGCCCC 
AATGACTGCC ACAACCGGGG TCACTGTGTA CAGGGCAAAT GCATATGTGA GCAAGGCTTC 
AAAGGCTTTG ACTGTAGTGA GATGAGCTGT CCCAATGACT GCCACCAGCA TGGCCGCTGT 
GTGAATGGCA TGTGCATCTG TGATGATGAC TACACTGGGG AAGACTGCAG AGACCGCCGC 
TGTCCCCGGG ACTGTAGCCA GCGGGGGCGC TGTGTGGACG GACAGTGCAT TTGTGAGGAT 
GGTTTCACTG GTTCTGACTG TGCTGAGCTC TCCTGCCCTG GTGACTGCCA CGGCCATGGC 
CGCTGTGTGA ATGGCCAATG CATCTGCCAC GAGGGCTTCA CCGGCRAAGA CTGCAAAGAG 
CAAAGGTGCC CCAGTGACTG CCATGGCCAA GGCCGCTGTG AGGACGGCCA ATGTATCTGC 
CATGAGGGCT TCACAGGCCT GGACTGTGGG CAGCGCTCCT GTCCCAATGA CTGCAGCAAC 
CAGGGACAAT GTGTGGCAGG CCGCTGCATC TGCAATGAAG GCTACACAGG GGTAGACTGC 
TCTGAGGTGT CCCCTCCCAA AGACCTTATT GTGACAGAAG TAACAGAGGA GACTGTARAT 
CTGGCATGGG ACAATGAGAT GCGGGTCACT GAGTACCTCA TTATGTACAC ACCCACCCAT 
GCTGATGGCC TGGAGATGCA GTTCCGTGTG CCTGGGGACC AGACATCTAC CACCATCCGG 
GAGCTGGAGC CAGGAGTGGA GTACTTCATT CGTGTGTTCG CCATCTTGGA GAACAAGAGG 
AGCATCCCTG TCAGTGCCAG AGTTGCCACC TACTTGCCTG CACCTGAAGG CCTAAAATTC 
AAGTCTATCA AGGAGACATC TGTGGAAGTA GAGTGGGATC CTCTGGACAT CGCTTTTGAA 
ACGTGGGAGA TCATTTTCAG AAATATGAAC AAAGAAGATG AGGGAGAGAT CACAAAAAGC 
TTGAGGAGGC CAGAGACCTC CTACCGCCAA ACTGGCCTGG CTCCTGGCCA AGAATATGAA 
ATATCTCTTC ACATTGTGAA AAACAACACC CGAGGCCCTG GCTTGAAGAA AGTGACCACA 
ACTCGCCTGG ATGCCCCCAG CCAAATTGAG GTGAGAGACA TCACAGACAC TACAGCACTG 
ATCACCTGGT CCAAGCCCTT GGCTGAAATT GATAGCATTG AGCTCTCCTA TGGCATCAAG 
GATGTGCCTG GAGACCGGAC CACCATCGAC CTCACACATG AAGACAACCA GTACTCCATC 
GGGAACCTGA AACCTGACAC GGAGTATGAG GTGTCCCTCA TCTCCCGCAG AGTGGACATG 
GCAAGCAACC CTGCCAAGGA GACCTTCATC ACAGGCCTGG ATGCTCCCAG GAATCTCCGT 
CGCGTCTCAC AGACAGACAA CAGCATCACC TTGGAGTGGA GGAATGTCAA GGCAGACATT 
GATAGTTATA GAATTAAGTA TGCACCTATC TCTGGAGGTG ACCATGCTGA GATAGATGTT 
CCAAAGAGCC AGCAAGCCAC AACCAAAACC ACACTCACAG GTCTAAGGCC AGGAACTGAA 
TATGGAGTGG GCGTCTCTGC TGTTAAGGGA GACAAGGAGG GCGATCCAGC AACCATCAAT 
GCAGCCACAG AAATTGATGC ACCCAGGGAC TTACAGGTGT CTGAGACCAC ACAAGACAGT 
CTGACTTTAT TCTGGAAGAC ACCTCTGGCC AAGTTTGATC GTTACCGCCT CAACTACAGC 
CTCCCCACAG GCCAGTCAAT AGAGATTCAG CTGCCAAAGG ATGCCACCTC CCATGTCCTG 
ACAGACCTGG AGCCAGGGCA AGAATACACT GTTCTCCTCA CCGCTGAGAA GGGCAGACAT 
AAGAGCAAGC CTGCGCGTGT GAAGGCATCC ACAGAAGAAG TACCTTCTCT GGAAAATCTC 
ACCGTGACTG AGGCCGGCTG GGATGGCCTC AGACTCAACT GGACTGCAGA TGACCTGGCC 
TATGAGTACT TTGTTATTCA GGTACAGGAA GCCAACAAGG TGGAGACTGC TCACAACTTC 
ACAGTACTTG GTAACCTCCG GACTGCAGAC ATCCCGGGCC TCAAGGCTGC CACTCCTTAT 
AGAGTCTCCA TTTATGGGGT AGCTCGGGGC TATAAAACAC CCGTGCTCTC TGCTGAGACC 
TTCACAGGGA AAACTCCCAG TTTGGGAGAG GTGACTGTGG CTGAGGTGGG CTGGGATGCC 
CTCAAGCTCA ACTGGACTGC TCCAGAAGGA GCCTATACGA ACTTTTTCAT TCAGGTGCTA 
GAGGCTGACA TGACCCAGAC TGTCCAGAAC CTCACAGTCC CAGGGGGACT GAGGTCAGTG 
GACCTACCTG GGCTCAAAGC AGCCACCCGC TATTACATCA CCGTTCGAGG GGTCACCCAG 
GACTTCAGCA CAGCCCCTCT CTCTGTTGAG GTCTTGACAG AGGAGATCCC TCAACTGGGA 
GGCTTGTCCG TGACTGAGGT CAGCTGGGAC AGCCTTACAC TCAACTGGAC CACAGATGAT 
CTGGCCTATA AGCACTTTAT CATTCAGGTG CAGGAGGCCA ACAATGTAGA GGCTGCTCAG 
AACCTCACAG TATCTGGTAG CCTCAGAGTT GTGGACATCC CAGGCCTCAA GGCTGATACC 
CCTTATAGAG TCTCCATCTA TGGGGTGATC CAGGGCTATA GAACACCAAT GCTCTCTGCT 
GATGTCTCCA CAGCCAAAGA ACCTGAAATT GGAAACTTAA ATATTTCTGA TGTAACTCCT 
GAGAGCTTCA ATCTCTCCTG GACAGCTACC GACGGGATCT TCGACATGTT TACTATCGAA 
ATTATTGATT CTAATAGGTT GCTGCAGACA GCAGAACATA ATATATCTGG TGCTGAACGA 
ACTGCCCACA TCTCGGGCCT TCCACCTAGT ACTGATTTCA TTGTCTACCT CTCTGGAATT 
GCTCCCAGCA TCCGTACCAA AACCATCAGT ACCACAGCCA CCACAGAAGC TGAACCGGAA 
GTTGACAACC TTCTAGTTTC AGATGCTACT CCAGACGGTT TCTGTCTGTC CTGGACTGCT 
GATGAAGGGA TATTCGACAG TTTTGTTATC AGGATCAGAG ATACCAAAAA GCAATCTGAA 
CCACAAGAAA TAACCCTCCC TTCCCCCGAC CGTACCAGGG ACATAACAGG TCTCAGAGAG 
GCCACTGAGT ACGAAATTGA ACTCTATGGA ATAAGCCGTG GAAGGCGATC CCAGCCAGTC 
AGTGCCATAG CAACAACAGC CATGGGCTCT CCGAAGGAAA TCATGTTCTC AGACATCACT 
GAAAATGCAG CCACAGTCAG CTGGAGGGCA CCGACTGCTC AGGTGGAGAG TTTCCGGATT 
ACTTATGTGC CTGTAACAGG AGGTCCCCCA TCCATGGTGA CCGTGGATGG GACGGATACT 
GAGACCCGAC TGGTGAGGCT CACCCCTGGA GTGGAGTACC ACGTCAGTGT GATTGCCATG 
AAGGGATTTG AAGAAAGTGA TCCGGTCTCG GGGTCTCTAA TCACAGCTCT CGATGGTCCA 
TCTGGCCTTC TGACAGCCAA CATCACAGAC TCAGAAGCCT TGGCCATGTG GCAGCCGGCC 
ATTGCCACTG TGGACAGTTA TGTCATCTCC TACACAGGGG AGAGAGTGCC AGAAATTACC 
CGCACAGTGT CTGGGAATAC AGTGGAGTTT GAACTACATG ACCTGGAGCC TGCCACAGAG 
TACACACTGA GTGTCTTTGC AGAGAAAGGT CACCAGAAGA GTTCTACCAT CGCTACGAAA 
TTTACCACAG ACCTGGATTC CCCAAGAGAA TTGACTGCTA CTGAGGTTCA GTCAGAAACT 
GCCTTCCTCA CCTGGAGACC TCCCCGGGCA TCGGTCACTG GATACCTCCT GGTCTATGAG 
TCTGTGGATG GTACAGTCAA GGAAGTCATT GTGGGGCCTG ACACCACCTC CTACAGCCTG 
GCAGACCTGA GCCCATCCAC CCACTACACA GCAAGAATCC AGGCATTGAG TGGGTCCCTG 
AGGAGCAAAC TGATCCAAAC CATCTTCACC ACAATTGGAC TCCTATATCC ATTCCCTAGG 
GATTGCTCTC AAGCAATGTT GAATGGCGAC ACCACCTCTG GCCTCTATAC CATCTATATA 
AATGGTGACA AGACTCAAGC ACTGGAAGTC TACTGTGATA TGACCTCTGA TGGAGGTGGA 
TGGATCGTTT TCCTGAGACG CAAAAATGGA CGTGAGGACT TCTATCGAAA CTGGAAGGCC 
TATGCCACTG GGTTTGGAGA CCGCAGAGAA GAATTCTGGC TTGGACTGGA TAACCTGAGC 
AAAATCACAG CCCAAGGGCA ATATGAGCTC CGGGTGGACC TACAAGACCA TGGGGAGTCA 
GCCTATGCTG TGTATGACAG GTTCAGTGTT GGAGATGCCA AGAGTCGCTA CAAGCTGAAG 
GTAGAAGGAT ACAGTGGAAC GGCAGGTGAC TCCATGAACT ATCACAATGG TAGATCCTTC 
TCTACCTATG ACAAGGACAC AGACTCAGCC ATCACCAACT GTGCCCTGTC CTACAAAGGA 
GCTTTCTGGT ATAAGAACTG TCATCGTGTC AACCTGATGG GCAGATATGG GGACAATAAT 
CACAGTCAGG GTGTTAACTG GTTCCACTGG AAGGGCCATG AGTACTCAAT CCAGTTTGCG 
GAGATGAAAC TAAGACCCAG CAACTTCCGA AATCTGGAAG GCAGGCGTAA GCGGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnc ( XM_008763754.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761976.1 Rattus norvegicus tenascin C (Tnc), transcript variant X1, mRNA.

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CloneID	ORa44020
Clone ID Related Accession (Same CDS sequence)	XM_008763754.2
Accession Version	XM_008763754.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tenascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763754.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCCG TGACCTGGCT ATTGCCAGGC ATCTTCCTAG CTTTGTTTGC CCTCACTCCT 
GAAGGTGGGG TCCTCAAGAA AGTCATCAGG CACAAGCGAG AGAGTGGGCT GAACTTGACC 
TTGCCAGAGG AGAATCAGCC AGTGGTGTTC AACCATATCT ACAACATCAA GTTGCCCATG 
GGTTCTCAGT GCTCAGTGGA TCTAGAGTCA ACGAGTGGAG AGAAAGACCT GAGCCCCACA 
CCAGAGTCCA GTGGAAGCTT CCAGGAGCAT ACAGTGGATG GGGAAAATCA GATTGTGTTC 
ACACACCGCA TCAACATCCC TCGTCGGGCC TGTGGCTGTG CCGCAGCTCC AGATGTGAAG 
GAGCTCCTGA GCAGACTGGA GGAACTGGAG ATGTTGGTAT CTTCTCTAAG GGAGCAGTGC 
ACCATGGGTA CAGGCTGTTG CCTCCAACCT GCAGAAGGCC GTCTGGACAG CAGGCCCTTC 
TGCAGCGGCA GGGGTAACTT CAGTGCTGAA GGTTGTGGCT GTGTCTGTGA ACCAGGCTGG 
AAAGGTCCCA ACTGCTCTGA GCCTGAATGC CCTGGAAACT GTAATCTCAG AGGCCAGTGC 
CTTGATGGAC AGTGTATCTG TGACCAGGGT TTCACTGGGG AAGACTGCAG CCAGCTAGCC 
TGTCCCAATG ACTGCAATGA CCAGGGCAAG TGTGTGAATG GGGTCTGTGT GTGCTTCGAA 
GGCTATGCCG GCCTTGACTG TGGCCTGGAA GTCTGCCCAG TGCCGTGCAG CGAGGAACAC 
GGGATGTGTG TGGATGGCAG GTGTGTGTGC AAAGATGGCT TTGCTGGTGA AGACTGCAAT 
GAGCCCCTTT GCTTCAACAA CTGCAACAAC CGTGGGCGGT GTGTGGAGAA CGAATGCGTC 
TGTGATGAGG GCTTCACGGG CGAAGACTGC AGCGAGCTCA TTTGCCCCAA TGACTGCTTC 
GACCGAGGTC GCTGCATCAA CGGCACCTGC TACTGTGAAG AAGGTTTCAC AGGTGAAGAC 
TGTGGTGAGC TCACCTGCCC CAACAACTGT CAGGGCCATG GCCAGTGTGA AGAGGGACAG 
TGTGTTTGCA ACGAGGGCTT TGCAGGGGCA GACTGCAGTG AAAAGCGGTG TCCCGCAGAC 
TGTCACCACC ATGGCCGCTG TCTCAACGGA CAGTGTGAGT GTGACGATGG GTTCACAGGG 
GCTGACTGTG GGGAACTCCA GTGCCCCAAT GGCTGCAGTG GGCATGGCCG CTGTGTCAAT 
GGGCAGTGTG TGTGTGACGA GGGCTACACC GGAGAAGACT GTAGCCAACG GCGATGCCCC 
AATGACTGCC ACAACCGGGG TCACTGTGTA CAGGGCAAAT GCATATGTGA GCAAGGCTTC 
AAAGGCTTTG ACTGTAGTGA GATGAGCTGT CCCAATGACT GCCACCAGCA TGGCCGCTGT 
GTGAATGGCA TGTGCATCTG TGATGATGAC TACACTGGGG AAGACTGCAG AGACCGCCGC 
TGTCCCCGGG ACTGTAGCCA GAGGGGGCGC TGTGTGGACG GACAGTGCAT TTGTGAGGAT 
GGTTTCACTG GTTCTGACTG TGCTGAGCTC TCCTGCCCTG GTGACTGCCA CGGCCATGGC 
CGCTGTGTGA ATGGCCAATG CATCTGCCAC GAGGGCTTCA CCGGCAAAGA CTGCAAAGAG 
CAAAGGTGCC CCAGTGACTG CCACGGCCAA GGCCGCTGTG AGGACGGCCA ATGTATCTGT 
CATGAGGGCT TCACAGGCCT GGACTGTGGG CAGCGCTCCT GTCCCAATGA CTGCAGCAAC 
CAGGGACAAT GTGTGGCAGG CCGCTGCATC TGCAATGAAG GCTACACAGG GGTAGACTGC 
TCTGAGGTGT CCCCTCCCAA AGACCTTATT GTGACAGAAG TAACAGAGGA GACTGTAAAT 
CTGGCATGGG ACAATGAGAT GCGGGTCACT GAGTACCTCA TTATGTACAC ACCCACCCAT 
GCTGATGGCC TGGAGATGCA GTTCCGTGTG CCTGGGGACC AGACATCTAC CACCATCCGG 
GAGCTGGAGC CAGGAGTGGA GTACTTCATT CATGTGTTCG CCATCTTGGA GAACAAGAGG 
AGCATCCCTG TCAGTGCCAG AGTTGCCACC TACTTGCCTG CACCTGAAGG CCTAAAATTC 
AAGTCTATCA AGGAGACATC TGTGGAAGTA GAGTGGGATC CTCTGGACAT CGCTTTTGAA 
ACGTGGGAGA TCATTTTCAG AAATATGAAC AAAGAAGATG AGGGAGAGAT CACAAAAAGC 
TTGAGGAGGC CAGAGACCTC CTACCGCCAA ACTGGCCTGG CTCCTGGCCA AGAATATGAA 
ATATCTCTTC ACATTGTGAA AAACAACACC CGAGGCCCTG GCTTGAAGAA AGTGACCACA 
ACTCGCCTGG ATGCCCCCAG CCAAATTGAG GTGAGAGACA TCACAGACAC TACAGCACTG 
ATCACCTGGT CCAAGCCCTT GGCTGAAATT GATAGCATTG AGCTCTCCTA TGGCATCAAG 
GATGTGCCTG GAGACCGGAC CACCATCGAC CTCACACATG AAGACAACCA GTACTCCATC 
GGGAACCTGA AACCTGACAC GGAGTATGAG GTGTCCCTCA TCTCCCGCAG AGTGGACATG 
GCAAGCAACC CTGCCAAGGA GACCTTCATC ACAGGCCTGG ATGCTCCCAG GAATCTCCGT 
CGCGTCTCAC AGACAGACAA CAGCATCACC TTGGAGTGGA GGAATGTCAA GGCAGACATT 
GATAGTTATA GAATTAAGTA TGCACCTATC TCTGGAGGTG ACCATGCTGA GATAGATGTT 
CCAAAGAGCC AGCAAGCCAC AACCAAAACC ACACTCACAG GTCTAAGGCC AGGAACTGAA 
TATGGAGTGG GCGTCTCTGC TGTTAAGGGA GACAAGGAGG GCGATCCAGC AACCATCAAT 
GCAGCCACAG AAATTGATGC ACCCAGGGAC TTACAGGTGT CTGAGACCAC ACAAGACAGT 
CTGACTTTAT TCTGGAAGAC ACCTCTGGCC AAGTTTGATC GTTACCGCCT CAACTACAGC 
CTCCCCACAG GCCAGTCAAT AGAGATTCAG CTGCCAAAGG ATGCCACCTC CCATGTCCTG 
ACAGACCTGG AGCCAGGGCA AGAATACACT GTTCTCCTCA CCGCTGAGAA GGGCAGACAT 
AAGAGCAAGC CTGCGCGTGT GAAGGCATCC ACAGAAGAAG TACCTTCTCT GGAAAATCTC 
ACCGTGACCG AGGCCGGCTG GGATGGCCTC AGACTCAACT GGACTGCAGA TGACCTGGCC 
TATGAGTACT TTGTTATTCA GGTACAGGAA GCCAACAAGG TGGAGACTGC TCACAACTTC 
ACAGTACTTG GTAACCTCCG GACTGCAGAC ATCCCGGGCC TCAAGGCTGC CACTCCTTAT 
AGAGTCTCCA TTTATGGGGT AGCTCGGGGC TATAAAACAC CCGTGCTCTC TGCTGAGACC 
TTCACAGGGA AAACTCCCAG TTTGGGAGAG GTGACTGTGG CTGAGGTGGG CTGGGATGCC 
CTCAAGCTCA ACTGGACTGC TCCAGAAGGA GCCTATACGA ACTTTTTCAT TCAGGTGCTA 
GAGGCTGACA TGACCCAGAC TGTCCAGAAC CTCACAGTCC CAGGGGGACT GAGGTCAGTG 
GACCTACCTG GGCTCAAAGC AGCCACCCGC TATTACATCA CCGTTCGAGG GGTCACCCAG 
GACTTCAGCA CAGCCCCTCT CTCTGTTGAG GTCTTGACAG AGGAGATCCC TCAACTGGGA 
GGCTTGTCCG TGACTGAGGT CAGCTGGGAC AGCCTTACAC TCAACTGGAC CACAGATGAT 
CTGGCCTATA AACACTTTAT CATTCAGGTG CAGGAGGCCA ACAATGTAGA GGCTGCTCAG 
AACCTCACAG TATCTGGTAG CCTCAGAGTT GTGGACATCC CAGGCCTCAA GGCTGATACC 
CCTTATAGAG TCTCCATCTA TGGGGTGATC CAGGGCTATA GAACACCAAT GCTCTCTGCT 
GATGTCTCCA CAGCCAAAGA ACCTGAAATT GGAAACTTAA ATATTTCTGA TGTAACTCCT 
GAGAGCTTCA ATCTCTCCTG GACAGCTACC GACGGGATCT TCGACATGTT TACTATCGAA 
ATTATTGATT CTAATAGGTT GCTGCAGACA GCAGAACATA ATATATCTGG TGCTGAACGA 
ACTGCCCACA TCTCGGGCCT TCCACCTAGT ACTGATTTCA TTGTCTACCT CTCTGGAATT 
GCTCCCAGCA TCCGTACCAA AACCATCAGT ACCACAGCCA CCACAGAGCC AGAACCACAG 
TTGGGCATGC TAATCTTTAG CAATATTACT CCTGACAGCT TCCATGTGTC ATGGACCACA 
CAAGCTGGGC TTTTTGCAAA GATTGTTATC AATGTGAGGG ACGCTCATTC ACTGCACGAG 
TCTCGGCAAA TCACAGTCCC GGGAGATGCA CAGCAAGCTC ACATCACAGG CTTGGTAGAG 
AACACTGCCT ATGATGTCAG TGTGACAGGC ACCACCTGGG CTGGTGACCC CACCAGACCC 
CTCACTGCCT TTGTCATTAC AGAGGCCTTG CCCCTTCTGG AAAATTTAAC CATTTCCGAC 
ACTAATCCCT ACGGGTTCAC AGTTTCCTGG ACGGCATCGG AGAATGCCTT TGACAGCTTT 
CTAGTAACGG TGGTGGATTC TGGGAAGCTG CTGGACCCCC AGGAATTCAC ACTTTCAGGA 
ACCCAGAGGA AGCTGGAGCT TAGAGGCCTC ATAACTGGCA TTGGCTATGA GGTCCTGGTC 
TCCGGCTTCA CCCAAGGGCA CCAAACAAAG CCCTTGAGGG CTGAGACCAT TACAGAAGCT 
GAACCGGAAG TTGACAACCT TCTAGTTTCA GATGCTACTC CAGACGGTTT CCGTCTGTCC 
TGGACTGCTG ATGAAGGGAT ATTCGACAGT TTTGTTATCA GGATCAGAGA TACCAAAAAG 
CAATCTGAAC CACAAGAAAT AACCCTCCCT TCCCCCGACC GTACCAGGGA CATAACAGGT 
CTCAGAGAGG CCACTGAGTA CGAAATTGAA CTCTATGGAA TAAGCCGTGG AAGGCGATCC 
CAGCCAGTCA GTGCCATAGC AACAACAGCC ATGGGTTCTC CGAAGGAAAT CATGTTCTCA 
GACATCACTG AAAATGCAGC CACAGTCAGC TGGAGAGCAC CGACTGCTCA AGTGGAGAGT 
TTCCGGATTA CTTATGTGCC TGTAACAGGA GGTCCCCCAT CCATGGTGAC CGTGGATGGG 
ACGGATACTG AGACCCGACT GGTGAGGCTC ACCCCTGGAG TGGAGTACCA CGTCAGTGTG 
ATTGCCATGA AGGGATTTGA AGAAAGTGAT CCGGTCTCGG GGTCTCTAAT CACAGCTCTC 
GATGGTCCAT CTGGCCTTCT GACAGCCAAC ATCACAGACT CAGAAGCCTT GGCCATGTGG 
CAGCCAGCCA TTGCCACTGT AGACAGTTAT GTCATCTCCT ACACAGGGGA GAGAGTGCCA 
GAAATTACCC GCACAGTGTC TGGGAATACA GTGGAGTTTG AACTACATGA CCTGGAGCCT 
GCCACAGAGT ACACACTGAG TGTCTTTGCA GAGAAAGGTC ACCAGAAGAG TTCTACCATC 
GCTACGAAAT TTACCACAGA CCTGGATTCC CCAAGAGAAT TGACTGCTAC TGAGGTTCAG 
TCAGAAACTG CCCTCCTCAC CTGGAGACCT CCCCGGGCAT CGGTCACTGG ATACCTCCTG 
GTCTATGAGT CTGTGGATGG TACAGTCAAG GAAGTCATTG TGGGGCCTGA CACCACCTCC 
TACAGCCTGG CAGACCTGAG CCCATCCACC CACTACACAG CAAGAATCCA GGCATTGAGT 
GGGTCCCTGA GGAGCAAACT GATCCAAACC ATCTTCACCA CAATTGGACT CCTATATCCA 
TTCCCTAGGG ATTGCTCTCA AGCAATGTTG AATGGCGACA CCACCTCTGG CCTCTATACC 
ATCTATATAA ATGGTGACAA GACTCAAGCA CTGGAAGTCT ACTGTGATAT GACCTCTGAT 
GGAGGTGGAT GGATCGTTTT CCTGAGACGC AAAAATGGAC GTGAGGACTT CTATCGAAAC 
TGGAAGGCCT ATGCCGCTGG GTTTGGAGAC CGCAGAGAAG AATTCTGGCT TGGACTGGAT 
AACCTGAGCA AAATCACAGC CCAAGGGCAA TATGAGCTCC GGGTGGACCT ACAAGACCAT 
GGGGAGTCAG CCTATGCTGT GTATGACAGG TTCAGTGTTG GAGATGCCAA GAGTCGCTAC 
AAGCTGAAGG TAGAAGGATA CAGTGGAACG GCAGGTGACT CCATGAACTA TCACAATGGT 
AGATCCTTCT CTACCTATGA CAAGGACACA GACTCAGCCA TCACCAACTG TGCCCTGTCC 
TACAAAGGAG CTTTCTGGTA TAAGAACTGT CATCGTGTCA ACCTGATGGG CAGATATGGG 
GACAATAATC ACAGTCAGGG TGTTAACTGG TTCCACTGGA AGGGCCATGA GTACTCAATC 
CAGTTTGCGG AGATGAAACT AAGACCCAGC AACTTCCGAA ATCTGGAAGG CAGGCGTAAG 
CGGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761976.1
CDS	380..6988
Translation	MGAVTWLLPGIFLALFALTPEGGVLKKVIRHKRESGLNLTLPEENQPVVFNHIYNIKLPMGSQCSVDLESTSGEKDLSPTPESSGSFQEHTVDGENQIVFTHRINIPRRACGCAAAPDVKELLSRLEELEMLVSSLREQCTMGTGCCLQPAEGRLDSRPFCSGRGNFSAEGCGCVCEPGWKGPNCSEPECPGNCNLRGQCLDGQCICDQGFTGEDCSQLACPNDCNDQGKCVNGVCVCFEGYAGLDCGLEVCPVPCSEEHGMCVDGRCVCKDGFAGEDCNEPLCFNNCNNRGRCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGELTCPNNCQGHGQCEEGQCVCNEGFAGADCSEKRCPADCHHHGRCLNGQCECDDGFTGADCGELQCPNGCSGHGRCVNGQCVCDEGYTGEDCSQRRCPNDCHNRGHCVQGKCICEQGFKGFDCSEMSCPNDCHQHGRCVNGMCICDDDYTGEDCRDRRCPRDCSQRGRCVDGQCICEDGFTGSDCAELSCPGDCHGHGRCVNGQCICHEGFTGKDCKEQRCPSDCHGQGRCEDGQCICHEGFTGLDCGQRSCPNDCSNQGQCVAGRCICNEGYTGVDCSEVSPPKDLIVTEVTEETVNLAWDNEMRVTEYLIMYTPTHADGLEMQFRVPGDQTSTTIRELEPGVEYFIHVFAILENKRSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEGEITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKKVTTTRLDAPSQIEVRDITDTTALITWSKPLAEIDSIELSYGIKDVPGDRTTIDLTHEDNQYSIGNLKPDTEYEVSLISRRVDMASNPAKETFITGLDAPRNLRRVSQTDNSITLEWRNVKADIDSYRIKYAPISGGDHAEIDVPKSQQATTKTTLTGLRPGTEYGVGVSAVKGDKEGDPATINAATEIDAPRDLQVSETTQDSLTLFWKTPLAKFDRYRLNYSLPTGQSIEIQLPKDATSHVLTDLEPGQEYTVLLTAEKGRHKSKPARVKASTEEVPSLENLTVTEAGWDGLRLNWTADDLAYEYFVIQVQEANKVETAHNFTVLGNLRTADIPGLKAATPYRVSIYGVARGYKTPVLSAETFTGKTPSLGEVTVAEVGWDALKLNWTAPEGAYTNFFIQVLEADMTQTVQNLTVPGGLRSVDLPGLKAATRYYITVRGVTQDFSTAPLSVEVLTEEIPQLGGLSVTEVSWDSLTLNWTTDDLAYKHFIIQVQEANNVEAAQNLTVSGSLRVVDIPGLKADTPYRVSIYGVIQGYRTPMLSADVSTAKEPEIGNLNISDVTPESFNLSWTATDGIFDMFTIEIIDSNRLLQTAEHNISGAERTAHISGLPPSTDFIVYLSGIAPSIRTKTISTTATTEPEPQLGMLIFSNITPDSFHVSWTTQAGLFAKIVINVRDAHSLHESRQITVPGDAQQAHITGLVENTAYDVSVTGTTWAGDPTRPLTAFVITEALPLLENLTISDTNPYGFTVSWTASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKLELRGLITGIGYEVLVSGFTQGHQTKPLRAETITEAEPEVDNLLVSDATPDGFRLSWTADEGIFDSFVIRIRDTKKQSEPQEITLPSPDRTRDITGLREATEYEIELYGISRGRRSQPVSAIATTAMGSPKEIMFSDITENAATVSWRAPTAQVESFRITYVPVTGGPPSMVTVDGTDTETRLVRLTPGVEYHVSVIAMKGFEESDPVSGSLITALDGPSGLLTANITDSEALAMWQPAIATVDSYVISYTGERVPEITRTVSGNTVEFELHDLEPATEYTLSVFAEKGHQKSSTIATKFTTDLDSPRELTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTARIQALSGSLRSKLIQTIFTTIGLLYPFPRDCSQAMLNGDTTSGLYTIYINGDKTQALEVYCDMTSDGGGWIVFLRRKNGREDFYRNWKAYAAGFGDRREEFWLGLDNLSKITAQGQYELRVDLQDHGESAYAVYDRFSVGDAKSRYKLKVEGYSGTAGDSMNYHNGRSFSTYDKDTDSAITNCALSYKGAFWYKNCHRVNLMGRYGDNNHSQGVNWFHWKGHEYSIQFAEMKLRPSNFRNLEGRRKRA

Target ORF information:

RefSeq Version	XM_008763754.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tenascin C (Tnc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763754.2

ORF Insert Sequence:

ATGGGGGCCG TGACCTGGCT ATTGCCAGGC ATCTTCCTAG CTTTGTTTGC CCTCACTCCT 
GAAGGTGGGG TCCTCAAGAA AGTCATCAGG CACAAGCGAG AGAGTGGGCT GAACTTGACC 
TTGCCAGAGG AGAATCAGCC AGTGGTGTTC AACCATATCT ACAACATCAA GTTGCCCATG 
GGTTCTCAGT GCTCAGTGGA TCTAGAGTCA ACGAGTGGAG AGAAAGACCT GAGCCCCACA 
CCAGAGTCCA GTGGAAGCTT CCAGGAGCAT ACAGTGGATG GGGAAAATCA GATTGTGTTC 
ACACACCGCA TCAACATCCC TCGTCGGGCC TGTGGCTGTG CCGCAGCTCC AGATGTGAAG 
GAGCTCCTGA GCAGACTGGA GGAACTGGAG ATGTTGGTAT CTTCTCTAAG GGAGCAGTGC 
ACCATGGGTA CAGGCTGTTG CCTCCAACCT GCAGAAGGCC GTCTGGACAG CAGGCCCTTC 
TGCAGCGGCA GGGGTAACTT CAGTGCTGAA GGTTGTGGCT GTGTCTGTGA ACCAGGCTGG 
AAAGGTCCCA ACTGCTCTGA GCCTGAATGC CCTGGAAACT GTAATCTCAG AGGCCAGTGC 
CTTGATGGAC AGTGTATCTG TGACCAGGGT TTCACTGGGG AAGACTGCAG CCAGCTAGCC 
TGTCCCAATG ACTGCAATGA CCAGGGCAAG TGTGTGAATG GGGTCTGTGT GTGCTTCGAA 
GGCTATGCCG GCCTTGACTG TGGCCTGGAA GTCTGCCCAG TGCCGTGCAG CGAGGAACAC 
GGGATGTGTG TGGATGGCAG GTGTGTGTGC AAAGATGGCT TTGCTGGTGA AGACTGCAAT 
GAGCCCCTTT GCTTCAACAA CTGCAACAAC CGTGGGCGGT GTGTGGAGAA CGAATGCGTC 
TGTGATGAGG GCTTCACGGG CGAAGACTGC AGCGAGCTCA TTTGCCCCAA TGACTGCTTC 
GACCGAGGTC GCTGCATCAA CGGCACCTGC TACTGTGAAG AAGGTTTCAC AGGTGAAGAC 
TGTGGTGAGC TCACCTGCCC CAACAACTGT CAGGGCCATG GCCAGTGTGA AGAGGGACAG 
TGTGTTTGCA ACGAGGGCTT TGCAGGGGCA GACTGCAGTG AAAAGCGGTG TCCCGCAGAC 
TGTCACCACC ATGGCCGCTG TCTCAACGGA CAGTGTGAGT GTGACGATGG GTTCACAGGG 
GCTGACTGTG GGGAACTCCA GTGCCCCAAT GGCTGCAGTG GGCATGGCCG CTGTGTCAAT 
GGGCAGTGTG TGTGTGACGA GGGCTACACC GGAGAAGACT GTAGCCAACG GCGATGCCCC 
AATGACTGCC ACAACCGGGG TCACTGTGTA CAGGGCAAAT GCATATGTGA GCAAGGCTTC 
AAAGGCTTTG ACTGTAGTGA GATGAGCTGT CCCAATGACT GCCACCAGCA TGGCCGCTGT 
GTGAATGGCA TGTGCATCTG TGATGATGAC TACACTGGGG AAGACTGCAG AGACCGCCGC 
TGTCCCCGGG ACTGTAGCCA GAGGGGGCGC TGTGTGGACG GACAGTGCAT TTGTGAGGAT 
GGTTTCACTG GTTCTGACTG TGCTGAGCTC TCCTGCCCTG GTGACTGCCA CGGCCATGGC 
CGCTGTGTGA ATGGCCAATG CATCTGCCAC GAGGGCTTCA CCGGCAAAGA CTGCAAAGAG 
CAAAGGTGCC CCAGTGACTG CCACGGCCAA GGCCGCTGTG AGGACGGCCA ATGTATCTGT 
CATGAGGGCT TCACAGGCCT GGACTGTGGG CAGCGCTCCT GTCCCAATGA CTGCAGCAAC 
CAGGGACAAT GTGTGGCAGG CCGCTGCATC TGCAATGAAG GCTACACAGG GGTAGACTGC 
TCTGAGGTGT CCCCTCCCAA AGACCTTATT GTGACAGAAG TAACAGAGGA GACTGTAAAT 
CTGGCATGGG ACAATGAGAT GCGGGTCACT GAGTACCTCA TTATGTACAC ACCCACCCAT 
GCTGATGGCC TGGAGATGCA GTTCCGTGTG CCTGGGGACC AGACATCTAC CACCATCCGG 
GAGCTGGAGC CAGGAGTGGA GTACTTCATT CATGTGTTCG CCATCTTGGA GAACAAGAGG 
AGCATCCCTG TCAGTGCCAG AGTTGCCACC TACTTGCCTG CACCTGAAGG CCTAAAATTC 
AAGTCTATCA AGGAGACATC TGTGGAAGTA GAGTGGGATC CTCTGGACAT CGCTTTTGAA 
ACGTGGGAGA TCATTTTCAG AAATATGAAC AAAGAAGATG AGGGAGAGAT CACAAAAAGC 
TTGAGGAGGC CAGAGACCTC CTACCGCCAA ACTGGCCTGG CTCCTGGCCA AGAATATGAA 
ATATCTCTTC ACATTGTGAA AAACAACACC CGAGGCCCTG GCTTGAAGAA AGTGACCACA 
ACTCGCCTGG ATGCCCCCAG CCAAATTGAG GTGAGAGACA TCACAGACAC TACAGCACTG 
ATCACCTGGT CCAAGCCCTT GGCTGAAATT GATAGCATTG AGCTCTCCTA TGGCATCAAG 
GATGTGCCTG GAGACCGGAC CACCATCGAC CTCACACATG AAGACAACCA GTACTCCATC 
GGGAACCTGA AACCTGACAC GGAGTATGAG GTGTCCCTCA TCTCCCGCAG AGTGGACATG 
GCAAGCAACC CTGCCAAGGA GACCTTCATC ACAGGCCTGG ATGCTCCCAG GAATCTCCGT 
CGCGTCTCAC AGACAGACAA CAGCATCACC TTGGAGTGGA GGAATGTCAA GGCAGACATT 
GATAGTTATA GAATTAAGTA TGCACCTATC TCTGGAGGTG ACCATGCTGA GATAGATGTT 
CCAAAGAGCC AGCAAGCCAC AACCAAAACC ACACTCACAG GTCTAAGGCC AGGAACTGAA 
TATGGAGTGG GCGTCTCTGC TGTTAAGGGA GACAAGGAGG GCGATCCAGC AACCATCAAT 
GCAGCCACAG AAATTGATGC ACCCAGGGAC TTACAGGTGT CTGAGACCAC ACAAGACAGT 
CTGACTTTAT TCTGGAAGAC ACCTCTGGCC AAGTTTGATC GTTACCGCCT CAACTACAGC 
CTCCCCACAG GCCAGTCAAT AGAGATTCAG CTGCCAAAGG ATGCCACCTC CCATGTCCTG 
ACAGACCTGG AGCCAGGGCA AGAATACACT GTTCTCCTCA CCGCTGAGAA GGGCAGACAT 
AAGAGCAAGC CTGCGCGTGT GAAGGCATCC ACAGAAGAAG TACCTTCTCT GGAAAATCTC 
ACCGTGACCG AGGCCGGCTG GGATGGCCTC AGACTCAACT GGACTGCAGA TGACCTGGCC 
TATGAGTACT TTGTTATTCA GGTACAGGAA GCCAACAAGG TGGAGACTGC TCACAACTTC 
ACAGTACTTG GTAACCTCCG GACTGCAGAC ATCCCGGGCC TCAAGGCTGC CACTCCTTAT 
AGAGTCTCCA TTTATGGGGT AGCTCGGGGC TATAAAACAC CCGTGCTCTC TGCTGAGACC 
TTCACAGGGA AAACTCCCAG TTTGGGAGAG GTGACTGTGG CTGAGGTGGG CTGGGATGCC 
CTCAAGCTCA ACTGGACTGC TCCAGAAGGA GCCTATACGA ACTTTTTCAT TCAGGTGCTA 
GAGGCTGACA TGACCCAGAC TGTCCAGAAC CTCACAGTCC CAGGGGGACT GAGGTCAGTG 
GACCTACCTG GGCTCAAAGC AGCCACCCGC TATTACATCA CCGTTCGAGG GGTCACCCAG 
GACTTCAGCA CAGCCCCTCT CTCTGTTGAG GTCTTGACAG AGGAGATCCC TCAACTGGGA 
GGCTTGTCCG TGACTGAGGT CAGCTGGGAC AGCCTTACAC TCAACTGGAC CACAGATGAT 
CTGGCCTATA AACACTTTAT CATTCAGGTG CAGGAGGCCA ACAATGTAGA GGCTGCTCAG 
AACCTCACAG TATCTGGTAG CCTCAGAGTT GTGGACATCC CAGGCCTCAA GGCTGATACC 
CCTTATAGAG TCTCCATCTA TGGGGTGATC CAGGGCTATA GAACACCAAT GCTCTCTGCT 
GATGTCTCCA CAGCCAAAGA ACCTGAAATT GGAAACTTAA ATATTTCTGA TGTAACTCCT 
GAGAGCTTCA ATCTCTCCTG GACAGCTACC GACGGGATCT TCGACATGTT TACTATCGAA 
ATTATTGATT CTAATAGGTT GCTGCAGACA GCAGAACATA ATATATCTGG TGCTGAACGA 
ACTGCCCACA TCTCGGGCCT TCCACCTAGT ACTGATTTCA TTGTCTACCT CTCTGGAATT 
GCTCCCAGCA TCCGTACCAA AACCATCAGT ACCACAGCCA CCACAGAGCC AGAACCACAG 
TTGGGCATGC TAATCTTTAG CAATATTACT CCTGACAGCT TCCATGTGTC ATGGACCACA 
CAAGCTGGGC TTTTTGCAAA GATTGTTATC AATGTGAGGG ACGCTCATTC ACTGCACGAG 
TCTCGGCAAA TCACAGTCCC GGGAGATGCA CAGCAAGCTC ACATCACAGG CTTGGTAGAG 
AACACTGCCT ATGATGTCAG TGTGACAGGC ACCACCTGGG CTGGTGACCC CACCAGACCC 
CTCACTGCCT TTGTCATTAC AGAGGCCTTG CCCCTTCTGG AAAATTTAAC CATTTCCGAC 
ACTAATCCCT ACGGGTTCAC AGTTTCCTGG ACGGCATCGG AGAATGCCTT TGACAGCTTT 
CTAGTAACGG TGGTGGATTC TGGGAAGCTG CTGGACCCCC AGGAATTCAC ACTTTCAGGA 
ACCCAGAGGA AGCTGGAGCT TAGAGGCCTC ATAACTGGCA TTGGCTATGA GGTCCTGGTC 
TCCGGCTTCA CCCAAGGGCA CCAAACAAAG CCCTTGAGGG CTGAGACCAT TACAGAAGCT 
GAACCGGAAG TTGACAACCT TCTAGTTTCA GATGCTACTC CAGACGGTTT CCGTCTGTCC 
TGGACTGCTG ATGAAGGGAT ATTCGACAGT TTTGTTATCA GGATCAGAGA TACCAAAAAG 
CAATCTGAAC CACAAGAAAT AACCCTCCCT TCCCCCGACC GTACCAGGGA CATAACAGGT 
CTCAGAGAGG CCACTGAGTA CGAAATTGAA CTCTATGGAA TAAGCCGTGG AAGGCGATCC 
CAGCCAGTCA GTGCCATAGC AACAACAGCC ATGGGTTCTC CGAAGGAAAT CATGTTCTCA 
GACATCACTG AAAATGCAGC CACAGTCAGC TGGAGAGCAC CGACTGCTCA AGTGGAGAGT 
TTCCGGATTA CTTATGTGCC TGTAACAGGA GGTCCCCCAT CCATGGTGAC CGTGGATGGG 
ACGGATACTG AGACCCGACT GGTGAGGCTC ACCCCTGGAG TGGAGTACCA CGTCAGTGTG 
ATTGCCATGA AGGGATTTGA AGAAAGTGAT CCGGTCTCGG GGTCTCTAAT CACAGCTCTC 
GATGGTCCAT CTGGCCTTCT GACAGCCAAC ATCACAGACT CAGAAGCCTT GGCCATGTGG 
CAGCCAGCCA TTGCCACTGT AGACAGTTAT GTCATCTCCT ACACAGGGGA GAGAGTGCCA 
GAAATTACCC GCACAGTGTC TGGGAATACA GTGGAGTTTG AACTACATGA CCTGGAGCCT 
GCCACAGAGT ACACACTGAG TGTCTTTGCA GAGAAAGGTC ACCAGAAGAG TTCTACCATC 
GCTACGAAAT TTACCACAGA CCTGGATTCC CCAAGAGAAT TGACTGCTAC TGAGGTTCAG 
TCAGAAACTG CCCTCCTCAC CTGGAGACCT CCCCGGGCAT CGGTCACTGG ATACCTCCTG 
GTCTATGAGT CTGTGGATGG TACAGTCAAG GAAGTCATTG TGGGGCCTGA CACCACCTCC 
TACAGCCTGG CAGACCTGAG CCCATCCACC CACTACACAG CAAGAATCCA GGCATTGAGT 
GGGTCCCTGA GGAGCAAACT GATCCAAACC ATCTTCACCA CAATTGGACT CCTATATCCA 
TTCCCTAGGG ATTGCTCTCA AGCAATGTTG AATGGCGACA CCACCTCTGG CCTCTATACC 
ATCTATATAA ATGGTGACAA GACTCAAGCA CTGGAAGTCT ACTGTGATAT GACCTCTGAT 
GGAGGTGGAT GGATCGTTTT CCTGAGACGC AAAAATGGAC GTGAGGACTT CTATCGAAAC 
TGGAAGGCCT ATGCCGCTGG GTTTGGAGAC CGCAGAGAAG AATTCTGGCT TGGACTGGAT 
AACCTGAGCA AAATCACAGC CCAAGGGCAA TATGAGCTCC GGGTGGACCT ACAAGACCAT 
GGGGAGTCAG CCTATGCTGT GTATGACAGG TTCAGTGTTG GAGATGCCAA GAGTCGCTAC 
AAGCTGAAGG TAGAAGGATA CAGTGGAACG GCAGGTGACT CCATGAACTA TCACAATGGT 
AGATCCTTCT CTACCTATGA CAAGGACACA GACTCAGCCA TCACCAACTG TGCCCTGTCC 
TACAAAGGAG CTTTCTGGTA TAAGAACTGT CATCGTGTCA ACCTGATGGG CAGATATGGG 
GACAATAATC ACAGTCAGGG TGTTAACTGG TTCCACTGGA AGGGCCATGA GTACTCAATC 
CAGTTTGCGG AGATGAAACT AAGACCCAGC AACTTCCGAA ATCTGGAAGG CAGGCGTAAG 
CGGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnc ( XM_008763755.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761977.1 Rattus norvegicus tenascin C (Tnc), transcript variant X2, mRNA.

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CloneID	ORa44021
Clone ID Related Accession (Same CDS sequence)	XM_008763755.2
Accession Version	XM_008763755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tenascin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCCG TGACCTGGCT ATTGCCAGGC ATCTTCCTAG CTTTGTTTGC CCTCACTCCT 
GAAGGTGGGG TCCTCAAGAA AGTCATCAGG CACAAGCGAG AGAGTGGGCT GAACTTGACC 
TTGCCAGAGG AGAATCAGCC AGTGGTGTTC AACCATATCT ACAACATCAA GTTGCCCATG 
GGTTCTCAGT GCTCAGTGGA TCTAGAGTCA ACGAGTGGAG AGAAAGACCT GAGCCCCACA 
CCAGAGTCCA GTGGAAGCTT CCAGGAGCAT ACAGTGGATG GGGAAAATCA GATTGTGTTC 
ACACACCGCA TCAACATCCC TCGTCGGGCC TGTGGCTGTG CCGCAGCTCC AGATGTGAAG 
GAGCTCCTGA GCAGACTGGA GGAACTGGAG ATGTTGGTAT CTTCTCTAAG GGAGCAGTGC 
ACCATGGGTA CAGGCTGTTG CCTCCAACCT GCAGAAGGCC GTCTGGACAG CAGGCCCTTC 
TGCAGCGGCA GGGGTAACTT CAGTGCTGAA GGTTGTGGCT GTGTCTGTGA ACCAGGCTGG 
AAAGGTCCCA ACTGCTCTGA GCCTGAATGC CCTGGAAACT GTAATCTCAG AGGCCAGTGC 
CTTGATGGAC AGTGTATCTG TGACCAGGGT TTCACTGGGG AAGACTGCAG CCAGCTAGCC 
TGTCCCAATG ACTGCAATGA CCAGGGCAAG TGTGTGAATG GGGTCTGTGT GTGCTTCGAA 
GGCTATGCCG GCCTTGACTG TGGCCTGGAA GTCTGCCCAG TGCCGTGCAG CGAGGAACAC 
GGGATGTGTG TGGATGGCAG GTGTGTGTGC AAAGATGGCT TTGCTGGTGA AGACTGCAAT 
GAGCCCCTTT GCTTCAACAA CTGCAACAAC CGTGGGCGGT GTGTGGAGAA CGAATGCGTC 
TGTGATGAGG GCTTCACGGG CGAAGACTGC AGCGAGCTCA TTTGCCCCAA TGACTGCTTC 
GACCGAGGTC GCTGCATCAA CGGCACCTGC TACTGTGAAG AAGGTTTCAC AGGTGAAGAC 
TGTGGTGAGC TCACCTGCCC CAACAACTGT CAGGGCCATG GCCAGTGTGA AGAGGGACAG 
TGTGTTTGCA ACGAGGGCTT TGCAGGGGCA GACTGCAGTG AAAAGCGGTG TCCCGCAGAC 
TGTCACCACC ATGGCCGCTG TCTCAACGGA CAGTGTGAGT GTGACGATGG GTTCACAGGG 
GCTGACTGTG GGGAACTCCA GTGCCCCAAT GGCTGCAGTG GGCATGGCCG CTGTGTCAAT 
GGGCAGTGTG TGTGTGACGA GGGCTACACC GGAGAAGACT GTAGCCAACG GCGATGCCCC 
AATGACTGCC ACAACCGGGG TCACTGTGTA CAGGGCAAAT GCATATGTGA GCAAGGCTTC 
AAAGGCTTTG ACTGTAGTGA GATGAGCTGT CCCAATGACT GCCACCAGCA TGGCCGCTGT 
GTGAATGGCA TGTGCATCTG TGATGATGAC TACACTGGGG AAGACTGCAG AGACCGCCGC 
TGTCCCCGGG ACTGTAGCCA GAGGGGGCGC TGTGTGGACG GACAGTGCAT TTGTGAGGAT 
GGTTTCACTG GTTCTGACTG TGCTGAGCTC TCCTGCCCTG GTGACTGCCA CGGCCATGGC 
CGCTGTGTGA ATGGCCAATG CATCTGCCAC GAGGGCTTCA CCGGCAAAGA CTGCAAAGAG 
CAAAGGTGCC CCAGTGACTG CCACGGCCAA GGCCGCTGTG AGGACGGCCA ATGTATCTGT 
CATGAGGGCT TCACAGGCCT GGACTGTGGG CAGCGCTCCT GTCCCAATGA CTGCAGCAAC 
CAGGGACAAT GTGTGGCAGG CCGCTGCATC TGCAATGAAG GCTACACAGG GGTAGACTGC 
TCTGAGGTGT CCCCTCCCAA AGACCTTATT GTGACAGAAG TAACAGAGGA GACTGTAAAT 
CTGGCATGGG ACAATGAGAT GCGGGTCACT GAGTACCTCA TTATGTACAC ACCCACCCAT 
GCTGATGGCC TGGAGATGCA GTTCCGTGTG CCTGGGGACC AGACATCTAC CACCATCCGG 
GAGCTGGAGC CAGGAGTGGA GTACTTCATT CATGTGTTCG CCATCTTGGA GAACAAGAGG 
AGCATCCCTG TCAGTGCCAG AGTTGCCACC TACTTGCCTG CACCTGAAGG CCTAAAATTC 
AAGTCTATCA AGGAGACATC TGTGGAAGTA GAGTGGGATC CTCTGGACAT CGCTTTTGAA 
ACGTGGGAGA TCATTTTCAG AAATATGAAC AAAGAAGATG AGGGAGAGAT CACAAAAAGC 
TTGAGGAGGC CAGAGACCTC CTACCGCCAA ACTGGCCTGG CTCCTGGCCA AGAATATGAA 
ATATCTCTTC ACATTGTGAA AAACAACACC CGAGGCCCTG GCTTGAAGAA AGTGACCACA 
ACTCGCCTGG ATGCCCCCAG CCAAATTGAG GTGAGAGACA TCACAGACAC TACAGCACTG 
ATCACCTGGT CCAAGCCCTT GGCTGAAATT GATAGCATTG AGCTCTCCTA TGGCATCAAG 
GATGTGCCTG GAGACCGGAC CACCATCGAC CTCACACATG AAGACAACCA GTACTCCATC 
GGGAACCTGA AACCTGACAC GGAGTATGAG GTGTCCCTCA TCTCCCGCAG AGTGGACATG 
GCAAGCAACC CTGCCAAGGA GACCTTCATC ACAGGCCTGG ATGCTCCCAG GAATCTCCGT 
CGCGTCTCAC AGACAGACAA CAGCATCACC TTGGAGTGGA GGAATGTCAA GGCAGACATT 
GATAGTTATA GAATTAAGTA TGCACCTATC TCTGGAGGTG ACCATGCTGA GATAGATGTT 
CCAAAGAGCC AGCAAGCCAC AACCAAAACC ACACTCACAG GTCTAAGGCC AGGAACTGAA 
TATGGAGTGG GCGTCTCTGC TGTTAAGGGA GACAAGGAGG GCGATCCAGC AACCATCAAT 
GCAGCCACAG AAATTGATGC ACCCAGGGAC TTACAGGTGT CTGAGACCAC ACAAGACAGT 
CTGACTTTAT TCTGGAAGAC ACCTCTGGCC AAGTTTGATC GTTACCGCCT CAACTACAGC 
CTCCCCACAG GCCAGTCAAT AGAGATTCAG CTGCCAAAGG ATGCCACCTC CCATGTCCTG 
ACAGACCTGG AGCCAGGGCA AGAATACACT GTTCTCCTCA CCGCTGAGAA GGGCAGACAT 
AAGAGCAAGC CTGCGCGTGT GAAGGCATCC ACAGAAGAAG TACCTTCTCT GGAAAATCTC 
ACCGTGACCG AGGCCGGCTG GGATGGCCTC AGACTCAACT GGACTGCAGA TGACCTGGCC 
TATGAGTACT TTGTTATTCA GGTACAGGAA GCCAACAAGG TGGAGACTGC TCACAACTTC 
ACAGTACTTG GTAACCTCCG GACTGCAGAC ATCCCGGGCC TCAAGGCTGC CACTCCTTAT 
AGAGTCTCCA TTTATGGGGT AGCTCGGGGC TATAAAACAC CCGTGCTCTC TGCTGAGACC 
TTCACAGGGA AAACTCCCAG TTTGGGAGAG GTGACTGTGG CTGAGGTGGG CTGGGATGCC 
CTCAAGCTCA ACTGGACTGC TCCAGAAGGA GCCTATACGA ACTTTTTCAT TCAGGTGCTA 
GAGGCTGACA TGACCCAGAC TGTCCAGAAC CTCACAGTCC CAGGGGGACT GAGGTCAGTG 
GACCTACCTG GGCTCAAAGC AGCCACCCGC TATTACATCA CCGTTCGAGG GGTCACCCAG 
GACTTCAGCA CAGCCCCTCT CTCTGTTGAG GTCTTGACAG CCAAAGAACC TGAAATTGGA 
AACTTAAATA TTTCTGATGT AACTCCTGAG AGCTTCAATC TCTCCTGGAC AGCTACCGAC 
GGGATCTTCG ACATGTTTAC TATCGAAATT ATTGATTCTA ATAGGTTGCT GCAGACAGCA 
GAACATAATA TATCTGGTGC TGAACGAACT GCCCACATCT CGGGCCTTCC ACCTAGTACT 
GATTTCATTG TCTACCTCTC TGGAATTGCT CCCAGCATCC GTACCAAAAC CATCAGTACC 
ACAGCCACCA CAGAGCCAGA ACCACAGTTG GGCATGCTAA TCTTTAGCAA TATTACTCCT 
GACAGCTTCC ATGTGTCATG GACCACACAA GCTGGGCTTT TTGCAAAGAT TGTTATCAAT 
GTGAGGGACG CTCATTCACT GCACGAGTCT CGGCAAATCA CAGTCCCGGG AGATGCACAG 
CAAGCTCACA TCACAGGCTT GGTAGAGAAC ACTGCCTATG ATGTCAGTGT GACAGGCACC 
ACCTGGGCTG GTGACCCCAC CAGACCCCTC ACTGCCTTTG TCATTACAGA GGCCTTGCCC 
CTTCTGGAAA ATTTAACCAT TTCCGACACT AATCCCTACG GGTTCACAGT TTCCTGGACG 
GCATCGGAGA ATGCCTTTGA CAGCTTTCTA GTAACGGTGG TGGATTCTGG GAAGCTGCTG 
GACCCCCAGG AATTCACACT TTCAGGAACC CAGAGGAAGC TGGAGCTTAG AGGCCTCATA 
ACTGGCATTG GCTATGAGGT CCTGGTCTCC GGCTTCACCC AAGGGCACCA AACAAAGCCC 
TTGAGGGCTG AGACCATTAC AGAAGCTGAA CCGGAAGTTG ACAACCTTCT AGTTTCAGAT 
GCTACTCCAG ACGGTTTCCG TCTGTCCTGG ACTGCTGATG AAGGGATATT CGACAGTTTT 
GTTATCAGGA TCAGAGATAC CAAAAAGCAA TCTGAACCAC AAGAAATAAC CCTCCCTTCC 
CCCGACCGTA CCAGGGACAT AACAGGTCTC AGAGAGGCCA CTGAGTACGA AATTGAACTC 
TATGGAATAA GCCGTGGAAG GCGATCCCAG CCAGTCAGTG CCATAGCAAC AACAGCCATG 
GGTTCTCCGA AGGAAATCAT GTTCTCAGAC ATCACTGAAA ATGCAGCCAC AGTCAGCTGG 
AGAGCACCGA CTGCTCAAGT GGAGAGTTTC CGGATTACTT ATGTGCCTGT AACAGGAGGT 
CCCCCATCCA TGGTGACCGT GGATGGGACG GATACTGAGA CCCGACTGGT GAGGCTCACC 
CCTGGAGTGG AGTACCACGT CAGTGTGATT GCCATGAAGG GATTTGAAGA AAGTGATCCG 
GTCTCGGGGT CTCTAATCAC AGCTCTCGAT GGTCCATCTG GCCTTCTGAC AGCCAACATC 
ACAGACTCAG AAGCCTTGGC CATGTGGCAG CCAGCCATTG CCACTGTAGA CAGTTATGTC 
ATCTCCTACA CAGGGGAGAG AGTGCCAGAA ATTACCCGCA CAGTGTCTGG GAATACAGTG 
GAGTTTGAAC TACATGACCT GGAGCCTGCC ACAGAGTACA CACTGAGTGT CTTTGCAGAG 
AAAGGTCACC AGAAGAGTTC TACCATCGCT ACGAAATTTA CCACAGACCT GGATTCCCCA 
AGAGAATTGA CTGCTACTGA GGTTCAGTCA GAAACTGCCC TCCTCACCTG GAGACCTCCC 
CGGGCATCGG TCACTGGATA CCTCCTGGTC TATGAGTCTG TGGATGGTAC AGTCAAGGAA 
GTCATTGTGG GGCCTGACAC CACCTCCTAC AGCCTGGCAG ACCTGAGCCC ATCCACCCAC 
TACACAGCAA GAATCCAGGC ATTGAGTGGG TCCCTGAGGA GCAAACTGAT CCAAACCATC 
TTCACCACAA TTGGACTCCT ATATCCATTC CCTAGGGATT GCTCTCAAGC AATGTTGAAT 
GGCGACACCA CCTCTGGCCT CTATACCATC TATATAAATG GTGACAAGAC TCAAGCACTG 
GAAGTCTACT GTGATATGAC CTCTGATGGA GGTGGATGGA TCGTTTTCCT GAGACGCAAA 
AATGGACGTG AGGACTTCTA TCGAAACTGG AAGGCCTATG CCGCTGGGTT TGGAGACCGC 
AGAGAAGAAT TCTGGCTTGG ACTGGATAAC CTGAGCAAAA TCACAGCCCA AGGGCAATAT 
GAGCTCCGGG TGGACCTACA AGACCATGGG GAGTCAGCCT ATGCTGTGTA TGACAGGTTC 
AGTGTTGGAG ATGCCAAGAG TCGCTACAAG CTGAAGGTAG AAGGATACAG TGGAACGGCA 
GGTGACTCCA TGAACTATCA CAATGGTAGA TCCTTCTCTA CCTATGACAA GGACACAGAC 
TCAGCCATCA CCAACTGTGC CCTGTCCTAC AAAGGAGCTT TCTGGTATAA GAACTGTCAT 
CGTGTCAACC TGATGGGCAG ATATGGGGAC AATAATCACA GTCAGGGTGT TAACTGGTTC 
CACTGGAAGG GCCATGAGTA CTCAATCCAG TTTGCGGAGA TGAAACTAAG ACCCAGCAAC 
TTCCGAAATC TGGAAGGCAG GCGTAAGCGG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761977.1
CDS	380..6715
Translation	MGAVTWLLPGIFLALFALTPEGGVLKKVIRHKRESGLNLTLPEENQPVVFNHIYNIKLPMGSQCSVDLESTSGEKDLSPTPESSGSFQEHTVDGENQIVFTHRINIPRRACGCAAAPDVKELLSRLEELEMLVSSLREQCTMGTGCCLQPAEGRLDSRPFCSGRGNFSAEGCGCVCEPGWKGPNCSEPECPGNCNLRGQCLDGQCICDQGFTGEDCSQLACPNDCNDQGKCVNGVCVCFEGYAGLDCGLEVCPVPCSEEHGMCVDGRCVCKDGFAGEDCNEPLCFNNCNNRGRCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGELTCPNNCQGHGQCEEGQCVCNEGFAGADCSEKRCPADCHHHGRCLNGQCECDDGFTGADCGELQCPNGCSGHGRCVNGQCVCDEGYTGEDCSQRRCPNDCHNRGHCVQGKCICEQGFKGFDCSEMSCPNDCHQHGRCVNGMCICDDDYTGEDCRDRRCPRDCSQRGRCVDGQCICEDGFTGSDCAELSCPGDCHGHGRCVNGQCICHEGFTGKDCKEQRCPSDCHGQGRCEDGQCICHEGFTGLDCGQRSCPNDCSNQGQCVAGRCICNEGYTGVDCSEVSPPKDLIVTEVTEETVNLAWDNEMRVTEYLIMYTPTHADGLEMQFRVPGDQTSTTIRELEPGVEYFIHVFAILENKRSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEGEITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKKVTTTRLDAPSQIEVRDITDTTALITWSKPLAEIDSIELSYGIKDVPGDRTTIDLTHEDNQYSIGNLKPDTEYEVSLISRRVDMASNPAKETFITGLDAPRNLRRVSQTDNSITLEWRNVKADIDSYRIKYAPISGGDHAEIDVPKSQQATTKTTLTGLRPGTEYGVGVSAVKGDKEGDPATINAATEIDAPRDLQVSETTQDSLTLFWKTPLAKFDRYRLNYSLPTGQSIEIQLPKDATSHVLTDLEPGQEYTVLLTAEKGRHKSKPARVKASTEEVPSLENLTVTEAGWDGLRLNWTADDLAYEYFVIQVQEANKVETAHNFTVLGNLRTADIPGLKAATPYRVSIYGVARGYKTPVLSAETFTGKTPSLGEVTVAEVGWDALKLNWTAPEGAYTNFFIQVLEADMTQTVQNLTVPGGLRSVDLPGLKAATRYYITVRGVTQDFSTAPLSVEVLTAKEPEIGNLNISDVTPESFNLSWTATDGIFDMFTIEIIDSNRLLQTAEHNISGAERTAHISGLPPSTDFIVYLSGIAPSIRTKTISTTATTEPEPQLGMLIFSNITPDSFHVSWTTQAGLFAKIVINVRDAHSLHESRQITVPGDAQQAHITGLVENTAYDVSVTGTTWAGDPTRPLTAFVITEALPLLENLTISDTNPYGFTVSWTASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKLELRGLITGIGYEVLVSGFTQGHQTKPLRAETITEAEPEVDNLLVSDATPDGFRLSWTADEGIFDSFVIRIRDTKKQSEPQEITLPSPDRTRDITGLREATEYEIELYGISRGRRSQPVSAIATTAMGSPKEIMFSDITENAATVSWRAPTAQVESFRITYVPVTGGPPSMVTVDGTDTETRLVRLTPGVEYHVSVIAMKGFEESDPVSGSLITALDGPSGLLTANITDSEALAMWQPAIATVDSYVISYTGERVPEITRTVSGNTVEFELHDLEPATEYTLSVFAEKGHQKSSTIATKFTTDLDSPRELTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTARIQALSGSLRSKLIQTIFTTIGLLYPFPRDCSQAMLNGDTTSGLYTIYINGDKTQALEVYCDMTSDGGGWIVFLRRKNGREDFYRNWKAYAAGFGDRREEFWLGLDNLSKITAQGQYELRVDLQDHGESAYAVYDRFSVGDAKSRYKLKVEGYSGTAGDSMNYHNGRSFSTYDKDTDSAITNCALSYKGAFWYKNCHRVNLMGRYGDNNHSQGVNWFHWKGHEYSIQFAEMKLRPSNFRNLEGRRKRA

Target ORF information:

RefSeq Version	XM_008763755.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tenascin C (Tnc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763755.2

ORF Insert Sequence:

ATGGGGGCCG TGACCTGGCT ATTGCCAGGC ATCTTCCTAG CTTTGTTTGC CCTCACTCCT 
GAAGGTGGGG TCCTCAAGAA AGTCATCAGG CACAAGCGAG AGAGTGGGCT GAACTTGACC 
TTGCCAGAGG AGAATCAGCC AGTGGTGTTC AACCATATCT ACAACATCAA GTTGCCCATG 
GGTTCTCAGT GCTCAGTGGA TCTAGAGTCA ACGAGTGGAG AGAAAGACCT GAGCCCCACA 
CCAGAGTCCA GTGGAAGCTT CCAGGAGCAT ACAGTGGATG GGGAAAATCA GATTGTGTTC 
ACACACCGCA TCAACATCCC TCGTCGGGCC TGTGGCTGTG CCGCAGCTCC AGATGTGAAG 
GAGCTCCTGA GCAGACTGGA GGAACTGGAG ATGTTGGTAT CTTCTCTAAG GGAGCAGTGC 
ACCATGGGTA CAGGCTGTTG CCTCCAACCT GCAGAAGGCC GTCTGGACAG CAGGCCCTTC 
TGCAGCGGCA GGGGTAACTT CAGTGCTGAA GGTTGTGGCT GTGTCTGTGA ACCAGGCTGG 
AAAGGTCCCA ACTGCTCTGA GCCTGAATGC CCTGGAAACT GTAATCTCAG AGGCCAGTGC 
CTTGATGGAC AGTGTATCTG TGACCAGGGT TTCACTGGGG AAGACTGCAG CCAGCTAGCC 
TGTCCCAATG ACTGCAATGA CCAGGGCAAG TGTGTGAATG GGGTCTGTGT GTGCTTCGAA 
GGCTATGCCG GCCTTGACTG TGGCCTGGAA GTCTGCCCAG TGCCGTGCAG CGAGGAACAC 
GGGATGTGTG TGGATGGCAG GTGTGTGTGC AAAGATGGCT TTGCTGGTGA AGACTGCAAT 
GAGCCCCTTT GCTTCAACAA CTGCAACAAC CGTGGGCGGT GTGTGGAGAA CGAATGCGTC 
TGTGATGAGG GCTTCACGGG CGAAGACTGC AGCGAGCTCA TTTGCCCCAA TGACTGCTTC 
GACCGAGGTC GCTGCATCAA CGGCACCTGC TACTGTGAAG AAGGTTTCAC AGGTGAAGAC 
TGTGGTGAGC TCACCTGCCC CAACAACTGT CAGGGCCATG GCCAGTGTGA AGAGGGACAG 
TGTGTTTGCA ACGAGGGCTT TGCAGGGGCA GACTGCAGTG AAAAGCGGTG TCCCGCAGAC 
TGTCACCACC ATGGCCGCTG TCTCAACGGA CAGTGTGAGT GTGACGATGG GTTCACAGGG 
GCTGACTGTG GGGAACTCCA GTGCCCCAAT GGCTGCAGTG GGCATGGCCG CTGTGTCAAT 
GGGCAGTGTG TGTGTGACGA GGGCTACACC GGAGAAGACT GTAGCCAACG GCGATGCCCC 
AATGACTGCC ACAACCGGGG TCACTGTGTA CAGGGCAAAT GCATATGTGA GCAAGGCTTC 
AAAGGCTTTG ACTGTAGTGA GATGAGCTGT CCCAATGACT GCCACCAGCA TGGCCGCTGT 
GTGAATGGCA TGTGCATCTG TGATGATGAC TACACTGGGG AAGACTGCAG AGACCGCCGC 
TGTCCCCGGG ACTGTAGCCA GAGGGGGCGC TGTGTGGACG GACAGTGCAT TTGTGAGGAT 
GGTTTCACTG GTTCTGACTG TGCTGAGCTC TCCTGCCCTG GTGACTGCCA CGGCCATGGC 
CGCTGTGTGA ATGGCCAATG CATCTGCCAC GAGGGCTTCA CCGGCAAAGA CTGCAAAGAG 
CAAAGGTGCC CCAGTGACTG CCACGGCCAA GGCCGCTGTG AGGACGGCCA ATGTATCTGT 
CATGAGGGCT TCACAGGCCT GGACTGTGGG CAGCGCTCCT GTCCCAATGA CTGCAGCAAC 
CAGGGACAAT GTGTGGCAGG CCGCTGCATC TGCAATGAAG GCTACACAGG GGTAGACTGC 
TCTGAGGTGT CCCCTCCCAA AGACCTTATT GTGACAGAAG TAACAGAGGA GACTGTAAAT 
CTGGCATGGG ACAATGAGAT GCGGGTCACT GAGTACCTCA TTATGTACAC ACCCACCCAT 
GCTGATGGCC TGGAGATGCA GTTCCGTGTG CCTGGGGACC AGACATCTAC CACCATCCGG 
GAGCTGGAGC CAGGAGTGGA GTACTTCATT CATGTGTTCG CCATCTTGGA GAACAAGAGG 
AGCATCCCTG TCAGTGCCAG AGTTGCCACC TACTTGCCTG CACCTGAAGG CCTAAAATTC 
AAGTCTATCA AGGAGACATC TGTGGAAGTA GAGTGGGATC CTCTGGACAT CGCTTTTGAA 
ACGTGGGAGA TCATTTTCAG AAATATGAAC AAAGAAGATG AGGGAGAGAT CACAAAAAGC 
TTGAGGAGGC CAGAGACCTC CTACCGCCAA ACTGGCCTGG CTCCTGGCCA AGAATATGAA 
ATATCTCTTC ACATTGTGAA AAACAACACC CGAGGCCCTG GCTTGAAGAA AGTGACCACA 
ACTCGCCTGG ATGCCCCCAG CCAAATTGAG GTGAGAGACA TCACAGACAC TACAGCACTG 
ATCACCTGGT CCAAGCCCTT GGCTGAAATT GATAGCATTG AGCTCTCCTA TGGCATCAAG 
GATGTGCCTG GAGACCGGAC CACCATCGAC CTCACACATG AAGACAACCA GTACTCCATC 
GGGAACCTGA AACCTGACAC GGAGTATGAG GTGTCCCTCA TCTCCCGCAG AGTGGACATG 
GCAAGCAACC CTGCCAAGGA GACCTTCATC ACAGGCCTGG ATGCTCCCAG GAATCTCCGT 
CGCGTCTCAC AGACAGACAA CAGCATCACC TTGGAGTGGA GGAATGTCAA GGCAGACATT 
GATAGTTATA GAATTAAGTA TGCACCTATC TCTGGAGGTG ACCATGCTGA GATAGATGTT 
CCAAAGAGCC AGCAAGCCAC AACCAAAACC ACACTCACAG GTCTAAGGCC AGGAACTGAA 
TATGGAGTGG GCGTCTCTGC TGTTAAGGGA GACAAGGAGG GCGATCCAGC AACCATCAAT 
GCAGCCACAG AAATTGATGC ACCCAGGGAC TTACAGGTGT CTGAGACCAC ACAAGACAGT 
CTGACTTTAT TCTGGAAGAC ACCTCTGGCC AAGTTTGATC GTTACCGCCT CAACTACAGC 
CTCCCCACAG GCCAGTCAAT AGAGATTCAG CTGCCAAAGG ATGCCACCTC CCATGTCCTG 
ACAGACCTGG AGCCAGGGCA AGAATACACT GTTCTCCTCA CCGCTGAGAA GGGCAGACAT 
AAGAGCAAGC CTGCGCGTGT GAAGGCATCC ACAGAAGAAG TACCTTCTCT GGAAAATCTC 
ACCGTGACCG AGGCCGGCTG GGATGGCCTC AGACTCAACT GGACTGCAGA TGACCTGGCC 
TATGAGTACT TTGTTATTCA GGTACAGGAA GCCAACAAGG TGGAGACTGC TCACAACTTC 
ACAGTACTTG GTAACCTCCG GACTGCAGAC ATCCCGGGCC TCAAGGCTGC CACTCCTTAT 
AGAGTCTCCA TTTATGGGGT AGCTCGGGGC TATAAAACAC CCGTGCTCTC TGCTGAGACC 
TTCACAGGGA AAACTCCCAG TTTGGGAGAG GTGACTGTGG CTGAGGTGGG CTGGGATGCC 
CTCAAGCTCA ACTGGACTGC TCCAGAAGGA GCCTATACGA ACTTTTTCAT TCAGGTGCTA 
GAGGCTGACA TGACCCAGAC TGTCCAGAAC CTCACAGTCC CAGGGGGACT GAGGTCAGTG 
GACCTACCTG GGCTCAAAGC AGCCACCCGC TATTACATCA CCGTTCGAGG GGTCACCCAG 
GACTTCAGCA CAGCCCCTCT CTCTGTTGAG GTCTTGACAG CCAAAGAACC TGAAATTGGA 
AACTTAAATA TTTCTGATGT AACTCCTGAG AGCTTCAATC TCTCCTGGAC AGCTACCGAC 
GGGATCTTCG ACATGTTTAC TATCGAAATT ATTGATTCTA ATAGGTTGCT GCAGACAGCA 
GAACATAATA TATCTGGTGC TGAACGAACT GCCCACATCT CGGGCCTTCC ACCTAGTACT 
GATTTCATTG TCTACCTCTC TGGAATTGCT CCCAGCATCC GTACCAAAAC CATCAGTACC 
ACAGCCACCA CAGAGCCAGA ACCACAGTTG GGCATGCTAA TCTTTAGCAA TATTACTCCT 
GACAGCTTCC ATGTGTCATG GACCACACAA GCTGGGCTTT TTGCAAAGAT TGTTATCAAT 
GTGAGGGACG CTCATTCACT GCACGAGTCT CGGCAAATCA CAGTCCCGGG AGATGCACAG 
CAAGCTCACA TCACAGGCTT GGTAGAGAAC ACTGCCTATG ATGTCAGTGT GACAGGCACC 
ACCTGGGCTG GTGACCCCAC CAGACCCCTC ACTGCCTTTG TCATTACAGA GGCCTTGCCC 
CTTCTGGAAA ATTTAACCAT TTCCGACACT AATCCCTACG GGTTCACAGT TTCCTGGACG 
GCATCGGAGA ATGCCTTTGA CAGCTTTCTA GTAACGGTGG TGGATTCTGG GAAGCTGCTG 
GACCCCCAGG AATTCACACT TTCAGGAACC CAGAGGAAGC TGGAGCTTAG AGGCCTCATA 
ACTGGCATTG GCTATGAGGT CCTGGTCTCC GGCTTCACCC AAGGGCACCA AACAAAGCCC 
TTGAGGGCTG AGACCATTAC AGAAGCTGAA CCGGAAGTTG ACAACCTTCT AGTTTCAGAT 
GCTACTCCAG ACGGTTTCCG TCTGTCCTGG ACTGCTGATG AAGGGATATT CGACAGTTTT 
GTTATCAGGA TCAGAGATAC CAAAAAGCAA TCTGAACCAC AAGAAATAAC CCTCCCTTCC 
CCCGACCGTA CCAGGGACAT AACAGGTCTC AGAGAGGCCA CTGAGTACGA AATTGAACTC 
TATGGAATAA GCCGTGGAAG GCGATCCCAG CCAGTCAGTG CCATAGCAAC AACAGCCATG 
GGTTCTCCGA AGGAAATCAT GTTCTCAGAC ATCACTGAAA ATGCAGCCAC AGTCAGCTGG 
AGAGCACCGA CTGCTCAAGT GGAGAGTTTC CGGATTACTT ATGTGCCTGT AACAGGAGGT 
CCCCCATCCA TGGTGACCGT GGATGGGACG GATACTGAGA CCCGACTGGT GAGGCTCACC 
CCTGGAGTGG AGTACCACGT CAGTGTGATT GCCATGAAGG GATTTGAAGA AAGTGATCCG 
GTCTCGGGGT CTCTAATCAC AGCTCTCGAT GGTCCATCTG GCCTTCTGAC AGCCAACATC 
ACAGACTCAG AAGCCTTGGC CATGTGGCAG CCAGCCATTG CCACTGTAGA CAGTTATGTC 
ATCTCCTACA CAGGGGAGAG AGTGCCAGAA ATTACCCGCA CAGTGTCTGG GAATACAGTG 
GAGTTTGAAC TACATGACCT GGAGCCTGCC ACAGAGTACA CACTGAGTGT CTTTGCAGAG 
AAAGGTCACC AGAAGAGTTC TACCATCGCT ACGAAATTTA CCACAGACCT GGATTCCCCA 
AGAGAATTGA CTGCTACTGA GGTTCAGTCA GAAACTGCCC TCCTCACCTG GAGACCTCCC 
CGGGCATCGG TCACTGGATA CCTCCTGGTC TATGAGTCTG TGGATGGTAC AGTCAAGGAA 
GTCATTGTGG GGCCTGACAC CACCTCCTAC AGCCTGGCAG ACCTGAGCCC ATCCACCCAC 
TACACAGCAA GAATCCAGGC ATTGAGTGGG TCCCTGAGGA GCAAACTGAT CCAAACCATC 
TTCACCACAA TTGGACTCCT ATATCCATTC CCTAGGGATT GCTCTCAAGC AATGTTGAAT 
GGCGACACCA CCTCTGGCCT CTATACCATC TATATAAATG GTGACAAGAC TCAAGCACTG 
GAAGTCTACT GTGATATGAC CTCTGATGGA GGTGGATGGA TCGTTTTCCT GAGACGCAAA 
AATGGACGTG AGGACTTCTA TCGAAACTGG AAGGCCTATG CCGCTGGGTT TGGAGACCGC 
AGAGAAGAAT TCTGGCTTGG ACTGGATAAC CTGAGCAAAA TCACAGCCCA AGGGCAATAT 
GAGCTCCGGG TGGACCTACA AGACCATGGG GAGTCAGCCT ATGCTGTGTA TGACAGGTTC 
AGTGTTGGAG ATGCCAAGAG TCGCTACAAG CTGAAGGTAG AAGGATACAG TGGAACGGCA 
GGTGACTCCA TGAACTATCA CAATGGTAGA TCCTTCTCTA CCTATGACAA GGACACAGAC 
TCAGCCATCA CCAACTGTGC CCTGTCCTAC AAAGGAGCTT TCTGGTATAA GAACTGTCAT 
CGTGTCAACC TGATGGGCAG ATATGGGGAC AATAATCACA GTCAGGGTGT TAACTGGTTC 
CACTGGAAGG GCCATGAGTA CTCAATCCAG TTTGCGGAGA TGAAACTAAG ACCCAGCAAC 
TTCCGAAATC TGGAAGGCAG GCGTAAGCGG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnc ( XM_008763756.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761978.1 Rattus norvegicus tenascin C (Tnc), transcript variant X3, mRNA.

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CloneID	ORa44022
Clone ID Related Accession (Same CDS sequence)	XM_008763756.2
Accession Version	XM_008763756.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tenascin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCCG TGACCTGGCT ATTGCCAGGC ATCTTCCTAG CTTTGTTTGC CCTCACTCCT 
GAAGGTGGGG TCCTCAAGAA AGTCATCAGG CACAAGCGAG AGAGTGGGCT GAACTTGACC 
TTGCCAGAGG AGAATCAGCC AGTGGTGTTC AACCATATCT ACAACATCAA GTTGCCCATG 
GGTTCTCAGT GCTCAGTGGA TCTAGAGTCA ACGAGTGGAG AGAAAGACCT GAGCCCCACA 
CCAGAGTCCA GTGGAAGCTT CCAGGAGCAT ACAGTGGATG GGGAAAATCA GATTGTGTTC 
ACACACCGCA TCAACATCCC TCGTCGGGCC TGTGGCTGTG CCGCAGCTCC AGATGTGAAG 
GAGCTCCTGA GCAGACTGGA GGAACTGGAG ATGTTGGTAT CTTCTCTAAG GGAGCAGTGC 
ACCATGGGTA CAGGCTGTTG CCTCCAACCT GCAGAAGGCC GTCTGGACAG CAGGCCCTTC 
TGCAGCGGCA GGGGTAACTT CAGTGCTGAA GGTTGTGGCT GTGTCTGTGA ACCAGGCTGG 
AAAGGTCCCA ACTGCTCTGA GCCTGAATGC CCTGGAAACT GTAATCTCAG AGGCCAGTGC 
CTTGATGGAC AGTGTATCTG TGACCAGGGT TTCACTGGGG AAGACTGCAG CCAGCTAGCC 
TGTCCCAATG ACTGCAATGA CCAGGGCAAG TGTGTGAATG GGGTCTGTGT GTGCTTCGAA 
GGCTATGCCG GCCTTGACTG TGGCCTGGAA GTCTGCCCAG TGCCGTGCAG CGAGGAACAC 
GGGATGTGTG TGGATGGCAG GTGTGTGTGC AAAGATGGCT TTGCTGGTGA AGACTGCAAT 
GAGCCCCTTT GCTTCAACAA CTGCAACAAC CGTGGGCGGT GTGTGGAGAA CGAATGCGTC 
TGTGATGAGG GCTTCACGGG CGAAGACTGC AGCGAGCTCA TTTGCCCCAA TGACTGCTTC 
GACCGAGGTC GCTGCATCAA CGGCACCTGC TACTGTGAAG AAGGTTTCAC AGGTGAAGAC 
TGTGGTGAGC TCACCTGCCC CAACAACTGT CAGGGCCATG GCCAGTGTGA AGAGGGACAG 
TGTGTTTGCA ACGAGGGCTT TGCAGGGGCA GACTGCAGTG AAAAGCGGTG TCCCGCAGAC 
TGTCACCACC ATGGCCGCTG TCTCAACGGA CAGTGTGAGT GTGACGATGG GTTCACAGGG 
GCTGACTGTG GGGAACTCCA GTGCCCCAAT GGCTGCAGTG GGCATGGCCG CTGTGTCAAT 
GGGCAGTGTG TGTGTGACGA GGGCTACACC GGAGAAGACT GTAGCCAACG GCGATGCCCC 
AATGACTGCC ACAACCGGGG TCACTGTGTA CAGGGCAAAT GCATATGTGA GCAAGGCTTC 
AAAGGCTTTG ACTGTAGTGA GATGAGCTGT CCCAATGACT GCCACCAGCA TGGCCGCTGT 
GTGAATGGCA TGTGCATCTG TGATGATGAC TACACTGGGG AAGACTGCAG AGACCGCCGC 
TGTCCCCGGG ACTGTAGCCA GAGGGGGCGC TGTGTGGACG GACAGTGCAT TTGTGAGGAT 
GGTTTCACTG GTTCTGACTG TGCTGAGCTC TCCTGCCCTG GTGACTGCCA CGGCCATGGC 
CGCTGTGTGA ATGGCCAATG CATCTGCCAC GAGGGCTTCA CCGGCAAAGA CTGCAAAGAG 
CAAAGGTGCC CCAGTGACTG CCACGGCCAA GGCCGCTGTG AGGACGGCCA ATGTATCTGT 
CATGAGGGCT TCACAGGCCT GGACTGTGGG CAGCGCTCCT GTCCCAATGA CTGCAGCAAC 
CAGGGACAAT GTGTGGCAGG CCGCTGCATC TGCAATGAAG GCTACACAGG GGTAGACTGC 
TCTGAGGTGT CCCCTCCCAA AGACCTTATT GTGACAGAAG TAACAGAGGA GACTGTAAAT 
CTGGCATGGG ACAATGAGAT GCGGGTCACT GAGTACCTCA TTATGTACAC ACCCACCCAT 
GCTGATGGCC TGGAGATGCA GTTCCGTGTG CCTGGGGACC AGACATCTAC CACCATCCGG 
GAGCTGGAGC CAGGAGTGGA GTACTTCATT CATGTGTTCG CCATCTTGGA GAACAAGAGG 
AGCATCCCTG TCAGTGCCAG AGTTGCCACC TACTTGCCTG CACCTGAAGG CCTAAAATTC 
AAGTCTATCA AGGAGACATC TGTGGAAGTA GAGTGGGATC CTCTGGACAT CGCTTTTGAA 
ACGTGGGAGA TCATTTTCAG AAATATGAAC AAAGAAGATG AGGGAGAGAT CACAAAAAGC 
TTGAGGAGGC CAGAGACCTC CTACCGCCAA ACTGGCCTGG CTCCTGGCCA AGAATATGAA 
ATATCTCTTC ACATTGTGAA AAACAACACC CGAGGCCCTG GCTTGAAGAA AGTGACCACA 
ACTCGCCTGG ATGCCCCCAG CCAAATTGAG GTGAGAGACA TCACAGACAC TACAGCACTG 
ATCACCTGGT CCAAGCCCTT GGCTGAAATT GATAGCATTG AGCTCTCCTA TGGCATCAAG 
GATGTGCCTG GAGACCGGAC CACCATCGAC CTCACACATG AAGACAACCA GTACTCCATC 
GGGAACCTGA AACCTGACAC GGAGTATGAG GTGTCCCTCA TCTCCCGCAG AGTGGACATG 
GCAAGCAACC CTGCCAAGGA GACCTTCATC ACAGGCCTGG ATGCTCCCAG GAATCTCCGT 
CGCGTCTCAC AGACAGACAA CAGCATCACC TTGGAGTGGA GGAATGTCAA GGCAGACATT 
GATAGTTATA GAATTAAGTA TGCACCTATC TCTGGAGGTG ACCATGCTGA GATAGATGTT 
CCAAAGAGCC AGCAAGCCAC AACCAAAACC ACACTCACAG GTCTAAGGCC AGGAACTGAA 
TATGGAGTGG GCGTCTCTGC TGTTAAGGGA GACAAGGAGG GCGATCCAGC AACCATCAAT 
GCAGCCACAG AAATTGATGC ACCCAGGGAC TTACAGGTGT CTGAGACCAC ACAAGACAGT 
CTGACTTTAT TCTGGAAGAC ACCTCTGGCC AAGTTTGATC GTTACCGCCT CAACTACAGC 
CTCCCCACAG GCCAGTCAAT AGAGATTCAG CTGCCAAAGG ATGCCACCTC CCATGTCCTG 
ACAGACCTGG AGCCAGGGCA AGAATACACT GTTCTCCTCA CCGCTGAGAA GGGCAGACAT 
AAGAGCAAGC CTGCGCGTGT GAAGGCATCC ACAGAAGAAG TACCTTCTCT GGAAAATCTC 
ACCGTGACCG AGGCCGGCTG GGATGGCCTC AGACTCAACT GGACTGCAGA TGACCTGGCC 
TATGAGTACT TTGTTATTCA GGTACAGGAA GCCAACAAGG TGGAGACTGC TCACAACTTC 
ACAGTACTTG GTAACCTCCG GACTGCAGAC ATCCCGGGCC TCAAGGCTGC CACTCCTTAT 
AGAGTCTCCA TTTATGGGGT AGCTCGGGGC TATAAAACAC CCGTGCTCTC TGCTGAGACC 
TTCACAGGGA AAACTCCCAG TTTGGGAGAG GTGACTGTGG CTGAGGTGGG CTGGGATGCC 
CTCAAGCTCA ACTGGACTGC TCCAGAAGGA GCCTATACGA ACTTTTTCAT TCAGGTGCTA 
GAGGCTGACA TGACCCAGAC TGTCCAGAAC CTCACAGTCC CAGGGGGACT GAGGTCAGTG 
GACCTACCTG GGCTCAAAGC AGCCACCCGC TATTACATCA CCGTTCGAGG GGTCACCCAG 
GACTTCAGCA CAGCCCCTCT CTCTGTTGAG GTCTTGACAG AGGAGATCCC TCAACTGGGA 
GGCTTGTCCG TGACTGAGGT CAGCTGGGAC AGCCTTACAC TCAACTGGAC CACAGATGAT 
CTGGCCTATA AACACTTTAT CATTCAGGTG CAGGAGGCCA ACAATGTAGA GGCTGCTCAG 
AACCTCACAG TATCTGGTAG CCTCAGAGTT GTGGACATCC CAGGCCTCAA GGCTGATACC 
CCTTATAGAG TCTCCATCTA TGGGGTGATC CAGGGCTATA GAACACCAAT GCTCTCTGCT 
GATGTCTCCA CAGCCAAAGA ACCTGAAATT GGAAACTTAA ATATTTCTGA TGTAACTCCT 
GAGAGCTTCA ATCTCTCCTG GACAGCTACC GACGGGATCT TCGACATGTT TACTATCGAA 
ATTATTGATT CTAATAGGTT GCTGCAGACA GCAGAACATA ATATATCTGG TGCTGAACGA 
ACTGCCCACA TCTCGGGCCT TCCACCTAGT ACTGATTTCA TTGTCTACCT CTCTGGAATT 
GCTCCCAGCA TCCGTACCAA AACCATCAGT ACCACAGCCA CCACAGAGGC CTTGCCCCTT 
CTGGAAAATT TAACCATTTC CGACACTAAT CCCTACGGGT TCACAGTTTC CTGGACGGCA 
TCGGAGAATG CCTTTGACAG CTTTCTAGTA ACGGTGGTGG ATTCTGGGAA GCTGCTGGAC 
CCCCAGGAAT TCACACTTTC AGGAACCCAG AGGAAGCTGG AGCTTAGAGG CCTCATAACT 
GGCATTGGCT ATGAGGTCCT GGTCTCCGGC TTCACCCAAG GGCACCAAAC AAAGCCCTTG 
AGGGCTGAGA CCATTACAGA AGCTGAACCG GAAGTTGACA ACCTTCTAGT TTCAGATGCT 
ACTCCAGACG GTTTCCGTCT GTCCTGGACT GCTGATGAAG GGATATTCGA CAGTTTTGTT 
ATCAGGATCA GAGATACCAA AAAGCAATCT GAACCACAAG AAATAACCCT CCCTTCCCCC 
GACCGTACCA GGGACATAAC AGGTCTCAGA GAGGCCACTG AGTACGAAAT TGAACTCTAT 
GGAATAAGCC GTGGAAGGCG ATCCCAGCCA GTCAGTGCCA TAGCAACAAC AGCCATGGGT 
TCTCCGAAGG AAATCATGTT CTCAGACATC ACTGAAAATG CAGCCACAGT CAGCTGGAGA 
GCACCGACTG CTCAAGTGGA GAGTTTCCGG ATTACTTATG TGCCTGTAAC AGGAGGTCCC 
CCATCCATGG TGACCGTGGA TGGGACGGAT ACTGAGACCC GACTGGTGAG GCTCACCCCT 
GGAGTGGAGT ACCACGTCAG TGTGATTGCC ATGAAGGGAT TTGAAGAAAG TGATCCGGTC 
TCGGGGTCTC TAATCACAGC TCTCGATGGT CCATCTGGCC TTCTGACAGC CAACATCACA 
GACTCAGAAG CCTTGGCCAT GTGGCAGCCA GCCATTGCCA CTGTAGACAG TTATGTCATC 
TCCTACACAG GGGAGAGAGT GCCAGAAATT ACCCGCACAG TGTCTGGGAA TACAGTGGAG 
TTTGAACTAC ATGACCTGGA GCCTGCCACA GAGTACACAC TGAGTGTCTT TGCAGAGAAA 
GGTCACCAGA AGAGTTCTAC CATCGCTACG AAATTTACCA CAGACCTGGA TTCCCCAAGA 
GAATTGACTG CTACTGAGGT TCAGTCAGAA ACTGCCCTCC TCACCTGGAG ACCTCCCCGG 
GCATCGGTCA CTGGATACCT CCTGGTCTAT GAGTCTGTGG ATGGTACAGT CAAGGAAGTC 
ATTGTGGGGC CTGACACCAC CTCCTACAGC CTGGCAGACC TGAGCCCATC CACCCACTAC 
ACAGCAAGAA TCCAGGCATT GAGTGGGTCC CTGAGGAGCA AACTGATCCA AACCATCTTC 
ACCACAATTG GACTCCTATA TCCATTCCCT AGGGATTGCT CTCAAGCAAT GTTGAATGGC 
GACACCACCT CTGGCCTCTA TACCATCTAT ATAAATGGTG ACAAGACTCA AGCACTGGAA 
GTCTACTGTG ATATGACCTC TGATGGAGGT GGATGGATCG TTTTCCTGAG ACGCAAAAAT 
GGACGTGAGG ACTTCTATCG AAACTGGAAG GCCTATGCCG CTGGGTTTGG AGACCGCAGA 
GAAGAATTCT GGCTTGGACT GGATAACCTG AGCAAAATCA CAGCCCAAGG GCAATATGAG 
CTCCGGGTGG ACCTACAAGA CCATGGGGAG TCAGCCTATG CTGTGTATGA CAGGTTCAGT 
GTTGGAGATG CCAAGAGTCG CTACAAGCTG AAGGTAGAAG GATACAGTGG AACGGCAGGT 
GACTCCATGA ACTATCACAA TGGTAGATCC TTCTCTACCT ATGACAAGGA CACAGACTCA 
GCCATCACCA ACTGTGCCCT GTCCTACAAA GGAGCTTTCT GGTATAAGAA CTGTCATCGT 
GTCAACCTGA TGGGCAGATA TGGGGACAAT AATCACAGTC AGGGTGTTAA CTGGTTCCAC 
TGGAAGGGCC ATGAGTACTC AATCCAGTTT GCGGAGATGA AACTAAGACC CAGCAACTTC 
CGAAATCTGG AAGGCAGGCG TAAGCGGGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761978.1
CDS	379..6711
Translation	MGAVTWLLPGIFLALFALTPEGGVLKKVIRHKRESGLNLTLPEENQPVVFNHIYNIKLPMGSQCSVDLESTSGEKDLSPTPESSGSFQEHTVDGENQIVFTHRINIPRRACGCAAAPDVKELLSRLEELEMLVSSLREQCTMGTGCCLQPAEGRLDSRPFCSGRGNFSAEGCGCVCEPGWKGPNCSEPECPGNCNLRGQCLDGQCICDQGFTGEDCSQLACPNDCNDQGKCVNGVCVCFEGYAGLDCGLEVCPVPCSEEHGMCVDGRCVCKDGFAGEDCNEPLCFNNCNNRGRCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGELTCPNNCQGHGQCEEGQCVCNEGFAGADCSEKRCPADCHHHGRCLNGQCECDDGFTGADCGELQCPNGCSGHGRCVNGQCVCDEGYTGEDCSQRRCPNDCHNRGHCVQGKCICEQGFKGFDCSEMSCPNDCHQHGRCVNGMCICDDDYTGEDCRDRRCPRDCSQRGRCVDGQCICEDGFTGSDCAELSCPGDCHGHGRCVNGQCICHEGFTGKDCKEQRCPSDCHGQGRCEDGQCICHEGFTGLDCGQRSCPNDCSNQGQCVAGRCICNEGYTGVDCSEVSPPKDLIVTEVTEETVNLAWDNEMRVTEYLIMYTPTHADGLEMQFRVPGDQTSTTIRELEPGVEYFIHVFAILENKRSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEGEITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKKVTTTRLDAPSQIEVRDITDTTALITWSKPLAEIDSIELSYGIKDVPGDRTTIDLTHEDNQYSIGNLKPDTEYEVSLISRRVDMASNPAKETFITGLDAPRNLRRVSQTDNSITLEWRNVKADIDSYRIKYAPISGGDHAEIDVPKSQQATTKTTLTGLRPGTEYGVGVSAVKGDKEGDPATINAATEIDAPRDLQVSETTQDSLTLFWKTPLAKFDRYRLNYSLPTGQSIEIQLPKDATSHVLTDLEPGQEYTVLLTAEKGRHKSKPARVKASTEEVPSLENLTVTEAGWDGLRLNWTADDLAYEYFVIQVQEANKVETAHNFTVLGNLRTADIPGLKAATPYRVSIYGVARGYKTPVLSAETFTGKTPSLGEVTVAEVGWDALKLNWTAPEGAYTNFFIQVLEADMTQTVQNLTVPGGLRSVDLPGLKAATRYYITVRGVTQDFSTAPLSVEVLTEEIPQLGGLSVTEVSWDSLTLNWTTDDLAYKHFIIQVQEANNVEAAQNLTVSGSLRVVDIPGLKADTPYRVSIYGVIQGYRTPMLSADVSTAKEPEIGNLNISDVTPESFNLSWTATDGIFDMFTIEIIDSNRLLQTAEHNISGAERTAHISGLPPSTDFIVYLSGIAPSIRTKTISTTATTEALPLLENLTISDTNPYGFTVSWTASENAFDSFLVTVVDSGKLLDPQEFTLSGTQRKLELRGLITGIGYEVLVSGFTQGHQTKPLRAETITEAEPEVDNLLVSDATPDGFRLSWTADEGIFDSFVIRIRDTKKQSEPQEITLPSPDRTRDITGLREATEYEIELYGISRGRRSQPVSAIATTAMGSPKEIMFSDITENAATVSWRAPTAQVESFRITYVPVTGGPPSMVTVDGTDTETRLVRLTPGVEYHVSVIAMKGFEESDPVSGSLITALDGPSGLLTANITDSEALAMWQPAIATVDSYVISYTGERVPEITRTVSGNTVEFELHDLEPATEYTLSVFAEKGHQKSSTIATKFTTDLDSPRELTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTARIQALSGSLRSKLIQTIFTTIGLLYPFPRDCSQAMLNGDTTSGLYTIYINGDKTQALEVYCDMTSDGGGWIVFLRRKNGREDFYRNWKAYAAGFGDRREEFWLGLDNLSKITAQGQYELRVDLQDHGESAYAVYDRFSVGDAKSRYKLKVEGYSGTAGDSMNYHNGRSFSTYDKDTDSAITNCALSYKGAFWYKNCHRVNLMGRYGDNNHSQGVNWFHWKGHEYSIQFAEMKLRPSNFRNLEGRRKRA

Target ORF information:

RefSeq Version	XM_008763756.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tenascin C (Tnc), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763756.2

ORF Insert Sequence:

ATGGGGGCCG TGACCTGGCT ATTGCCAGGC ATCTTCCTAG CTTTGTTTGC CCTCACTCCT 
GAAGGTGGGG TCCTCAAGAA AGTCATCAGG CACAAGCGAG AGAGTGGGCT GAACTTGACC 
TTGCCAGAGG AGAATCAGCC AGTGGTGTTC AACCATATCT ACAACATCAA GTTGCCCATG 
GGTTCTCAGT GCTCAGTGGA TCTAGAGTCA ACGAGTGGAG AGAAAGACCT GAGCCCCACA 
CCAGAGTCCA GTGGAAGCTT CCAGGAGCAT ACAGTGGATG GGGAAAATCA GATTGTGTTC 
ACACACCGCA TCAACATCCC TCGTCGGGCC TGTGGCTGTG CCGCAGCTCC AGATGTGAAG 
GAGCTCCTGA GCAGACTGGA GGAACTGGAG ATGTTGGTAT CTTCTCTAAG GGAGCAGTGC 
ACCATGGGTA CAGGCTGTTG CCTCCAACCT GCAGAAGGCC GTCTGGACAG CAGGCCCTTC 
TGCAGCGGCA GGGGTAACTT CAGTGCTGAA GGTTGTGGCT GTGTCTGTGA ACCAGGCTGG 
AAAGGTCCCA ACTGCTCTGA GCCTGAATGC CCTGGAAACT GTAATCTCAG AGGCCAGTGC 
CTTGATGGAC AGTGTATCTG TGACCAGGGT TTCACTGGGG AAGACTGCAG CCAGCTAGCC 
TGTCCCAATG ACTGCAATGA CCAGGGCAAG TGTGTGAATG GGGTCTGTGT GTGCTTCGAA 
GGCTATGCCG GCCTTGACTG TGGCCTGGAA GTCTGCCCAG TGCCGTGCAG CGAGGAACAC 
GGGATGTGTG TGGATGGCAG GTGTGTGTGC AAAGATGGCT TTGCTGGTGA AGACTGCAAT 
GAGCCCCTTT GCTTCAACAA CTGCAACAAC CGTGGGCGGT GTGTGGAGAA CGAATGCGTC 
TGTGATGAGG GCTTCACGGG CGAAGACTGC AGCGAGCTCA TTTGCCCCAA TGACTGCTTC 
GACCGAGGTC GCTGCATCAA CGGCACCTGC TACTGTGAAG AAGGTTTCAC AGGTGAAGAC 
TGTGGTGAGC TCACCTGCCC CAACAACTGT CAGGGCCATG GCCAGTGTGA AGAGGGACAG 
TGTGTTTGCA ACGAGGGCTT TGCAGGGGCA GACTGCAGTG AAAAGCGGTG TCCCGCAGAC 
TGTCACCACC ATGGCCGCTG TCTCAACGGA CAGTGTGAGT GTGACGATGG GTTCACAGGG 
GCTGACTGTG GGGAACTCCA GTGCCCCAAT GGCTGCAGTG GGCATGGCCG CTGTGTCAAT 
GGGCAGTGTG TGTGTGACGA GGGCTACACC GGAGAAGACT GTAGCCAACG GCGATGCCCC 
AATGACTGCC ACAACCGGGG TCACTGTGTA CAGGGCAAAT GCATATGTGA GCAAGGCTTC 
AAAGGCTTTG ACTGTAGTGA GATGAGCTGT CCCAATGACT GCCACCAGCA TGGCCGCTGT 
GTGAATGGCA TGTGCATCTG TGATGATGAC TACACTGGGG AAGACTGCAG AGACCGCCGC 
TGTCCCCGGG ACTGTAGCCA GAGGGGGCGC TGTGTGGACG GACAGTGCAT TTGTGAGGAT 
GGTTTCACTG GTTCTGACTG TGCTGAGCTC TCCTGCCCTG GTGACTGCCA CGGCCATGGC 
CGCTGTGTGA ATGGCCAATG CATCTGCCAC GAGGGCTTCA CCGGCAAAGA CTGCAAAGAG 
CAAAGGTGCC CCAGTGACTG CCACGGCCAA GGCCGCTGTG AGGACGGCCA ATGTATCTGT 
CATGAGGGCT TCACAGGCCT GGACTGTGGG CAGCGCTCCT GTCCCAATGA CTGCAGCAAC 
CAGGGACAAT GTGTGGCAGG CCGCTGCATC TGCAATGAAG GCTACACAGG GGTAGACTGC 
TCTGAGGTGT CCCCTCCCAA AGACCTTATT GTGACAGAAG TAACAGAGGA GACTGTAAAT 
CTGGCATGGG ACAATGAGAT GCGGGTCACT GAGTACCTCA TTATGTACAC ACCCACCCAT 
GCTGATGGCC TGGAGATGCA GTTCCGTGTG CCTGGGGACC AGACATCTAC CACCATCCGG 
GAGCTGGAGC CAGGAGTGGA GTACTTCATT CATGTGTTCG CCATCTTGGA GAACAAGAGG 
AGCATCCCTG TCAGTGCCAG AGTTGCCACC TACTTGCCTG CACCTGAAGG CCTAAAATTC 
AAGTCTATCA AGGAGACATC TGTGGAAGTA GAGTGGGATC CTCTGGACAT CGCTTTTGAA 
ACGTGGGAGA TCATTTTCAG AAATATGAAC AAAGAAGATG AGGGAGAGAT CACAAAAAGC 
TTGAGGAGGC CAGAGACCTC CTACCGCCAA ACTGGCCTGG CTCCTGGCCA AGAATATGAA 
ATATCTCTTC ACATTGTGAA AAACAACACC CGAGGCCCTG GCTTGAAGAA AGTGACCACA 
ACTCGCCTGG ATGCCCCCAG CCAAATTGAG GTGAGAGACA TCACAGACAC TACAGCACTG 
ATCACCTGGT CCAAGCCCTT GGCTGAAATT GATAGCATTG AGCTCTCCTA TGGCATCAAG 
GATGTGCCTG GAGACCGGAC CACCATCGAC CTCACACATG AAGACAACCA GTACTCCATC 
GGGAACCTGA AACCTGACAC GGAGTATGAG GTGTCCCTCA TCTCCCGCAG AGTGGACATG 
GCAAGCAACC CTGCCAAGGA GACCTTCATC ACAGGCCTGG ATGCTCCCAG GAATCTCCGT 
CGCGTCTCAC AGACAGACAA CAGCATCACC TTGGAGTGGA GGAATGTCAA GGCAGACATT 
GATAGTTATA GAATTAAGTA TGCACCTATC TCTGGAGGTG ACCATGCTGA GATAGATGTT 
CCAAAGAGCC AGCAAGCCAC AACCAAAACC ACACTCACAG GTCTAAGGCC AGGAACTGAA 
TATGGAGTGG GCGTCTCTGC TGTTAAGGGA GACAAGGAGG GCGATCCAGC AACCATCAAT 
GCAGCCACAG AAATTGATGC ACCCAGGGAC TTACAGGTGT CTGAGACCAC ACAAGACAGT 
CTGACTTTAT TCTGGAAGAC ACCTCTGGCC AAGTTTGATC GTTACCGCCT CAACTACAGC 
CTCCCCACAG GCCAGTCAAT AGAGATTCAG CTGCCAAAGG ATGCCACCTC CCATGTCCTG 
ACAGACCTGG AGCCAGGGCA AGAATACACT GTTCTCCTCA CCGCTGAGAA GGGCAGACAT 
AAGAGCAAGC CTGCGCGTGT GAAGGCATCC ACAGAAGAAG TACCTTCTCT GGAAAATCTC 
ACCGTGACCG AGGCCGGCTG GGATGGCCTC AGACTCAACT GGACTGCAGA TGACCTGGCC 
TATGAGTACT TTGTTATTCA GGTACAGGAA GCCAACAAGG TGGAGACTGC TCACAACTTC 
ACAGTACTTG GTAACCTCCG GACTGCAGAC ATCCCGGGCC TCAAGGCTGC CACTCCTTAT 
AGAGTCTCCA TTTATGGGGT AGCTCGGGGC TATAAAACAC CCGTGCTCTC TGCTGAGACC 
TTCACAGGGA AAACTCCCAG TTTGGGAGAG GTGACTGTGG CTGAGGTGGG CTGGGATGCC 
CTCAAGCTCA ACTGGACTGC TCCAGAAGGA GCCTATACGA ACTTTTTCAT TCAGGTGCTA 
GAGGCTGACA TGACCCAGAC TGTCCAGAAC CTCACAGTCC CAGGGGGACT GAGGTCAGTG 
GACCTACCTG GGCTCAAAGC AGCCACCCGC TATTACATCA CCGTTCGAGG GGTCACCCAG 
GACTTCAGCA CAGCCCCTCT CTCTGTTGAG GTCTTGACAG AGGAGATCCC TCAACTGGGA 
GGCTTGTCCG TGACTGAGGT CAGCTGGGAC AGCCTTACAC TCAACTGGAC CACAGATGAT 
CTGGCCTATA AACACTTTAT CATTCAGGTG CAGGAGGCCA ACAATGTAGA GGCTGCTCAG 
AACCTCACAG TATCTGGTAG CCTCAGAGTT GTGGACATCC CAGGCCTCAA GGCTGATACC 
CCTTATAGAG TCTCCATCTA TGGGGTGATC CAGGGCTATA GAACACCAAT GCTCTCTGCT 
GATGTCTCCA CAGCCAAAGA ACCTGAAATT GGAAACTTAA ATATTTCTGA TGTAACTCCT 
GAGAGCTTCA ATCTCTCCTG GACAGCTACC GACGGGATCT TCGACATGTT TACTATCGAA 
ATTATTGATT CTAATAGGTT GCTGCAGACA GCAGAACATA ATATATCTGG TGCTGAACGA 
ACTGCCCACA TCTCGGGCCT TCCACCTAGT ACTGATTTCA TTGTCTACCT CTCTGGAATT 
GCTCCCAGCA TCCGTACCAA AACCATCAGT ACCACAGCCA CCACAGAGGC CTTGCCCCTT 
CTGGAAAATT TAACCATTTC CGACACTAAT CCCTACGGGT TCACAGTTTC CTGGACGGCA 
TCGGAGAATG CCTTTGACAG CTTTCTAGTA ACGGTGGTGG ATTCTGGGAA GCTGCTGGAC 
CCCCAGGAAT TCACACTTTC AGGAACCCAG AGGAAGCTGG AGCTTAGAGG CCTCATAACT 
GGCATTGGCT ATGAGGTCCT GGTCTCCGGC TTCACCCAAG GGCACCAAAC AAAGCCCTTG 
AGGGCTGAGA CCATTACAGA AGCTGAACCG GAAGTTGACA ACCTTCTAGT TTCAGATGCT 
ACTCCAGACG GTTTCCGTCT GTCCTGGACT GCTGATGAAG GGATATTCGA CAGTTTTGTT 
ATCAGGATCA GAGATACCAA AAAGCAATCT GAACCACAAG AAATAACCCT CCCTTCCCCC 
GACCGTACCA GGGACATAAC AGGTCTCAGA GAGGCCACTG AGTACGAAAT TGAACTCTAT 
GGAATAAGCC GTGGAAGGCG ATCCCAGCCA GTCAGTGCCA TAGCAACAAC AGCCATGGGT 
TCTCCGAAGG AAATCATGTT CTCAGACATC ACTGAAAATG CAGCCACAGT CAGCTGGAGA 
GCACCGACTG CTCAAGTGGA GAGTTTCCGG ATTACTTATG TGCCTGTAAC AGGAGGTCCC 
CCATCCATGG TGACCGTGGA TGGGACGGAT ACTGAGACCC GACTGGTGAG GCTCACCCCT 
GGAGTGGAGT ACCACGTCAG TGTGATTGCC ATGAAGGGAT TTGAAGAAAG TGATCCGGTC 
TCGGGGTCTC TAATCACAGC TCTCGATGGT CCATCTGGCC TTCTGACAGC CAACATCACA 
GACTCAGAAG CCTTGGCCAT GTGGCAGCCA GCCATTGCCA CTGTAGACAG TTATGTCATC 
TCCTACACAG GGGAGAGAGT GCCAGAAATT ACCCGCACAG TGTCTGGGAA TACAGTGGAG 
TTTGAACTAC ATGACCTGGA GCCTGCCACA GAGTACACAC TGAGTGTCTT TGCAGAGAAA 
GGTCACCAGA AGAGTTCTAC CATCGCTACG AAATTTACCA CAGACCTGGA TTCCCCAAGA 
GAATTGACTG CTACTGAGGT TCAGTCAGAA ACTGCCCTCC TCACCTGGAG ACCTCCCCGG 
GCATCGGTCA CTGGATACCT CCTGGTCTAT GAGTCTGTGG ATGGTACAGT CAAGGAAGTC 
ATTGTGGGGC CTGACACCAC CTCCTACAGC CTGGCAGACC TGAGCCCATC CACCCACTAC 
ACAGCAAGAA TCCAGGCATT GAGTGGGTCC CTGAGGAGCA AACTGATCCA AACCATCTTC 
ACCACAATTG GACTCCTATA TCCATTCCCT AGGGATTGCT CTCAAGCAAT GTTGAATGGC 
GACACCACCT CTGGCCTCTA TACCATCTAT ATAAATGGTG ACAAGACTCA AGCACTGGAA 
GTCTACTGTG ATATGACCTC TGATGGAGGT GGATGGATCG TTTTCCTGAG ACGCAAAAAT 
GGACGTGAGG ACTTCTATCG AAACTGGAAG GCCTATGCCG CTGGGTTTGG AGACCGCAGA 
GAAGAATTCT GGCTTGGACT GGATAACCTG AGCAAAATCA CAGCCCAAGG GCAATATGAG 
CTCCGGGTGG ACCTACAAGA CCATGGGGAG TCAGCCTATG CTGTGTATGA CAGGTTCAGT 
GTTGGAGATG CCAAGAGTCG CTACAAGCTG AAGGTAGAAG GATACAGTGG AACGGCAGGT 
GACTCCATGA ACTATCACAA TGGTAGATCC TTCTCTACCT ATGACAAGGA CACAGACTCA 
GCCATCACCA ACTGTGCCCT GTCCTACAAA GGAGCTTTCT GGTATAAGAA CTGTCATCGT 
GTCAACCTGA TGGGCAGATA TGGGGACAAT AATCACAGTC AGGGTGTTAA CTGGTTCCAC 
TGGAAGGGCC ATGAGTACTC AATCCAGTTT GCGGAGATGA AACTAAGACC CAGCAACTTC 
CGAAATCTGG AAGGCAGGCG TAAGCGGGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnc ( XM_008763757.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761979.1 Rattus norvegicus tenascin C (Tnc), transcript variant X4, mRNA.

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CloneID	ORa44023
Clone ID Related Accession (Same CDS sequence)	XM_008763757.2
Accession Version	XM_008763757.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tenascin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763757.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCCG TGACCTGGCT ATTGCCAGGC ATCTTCCTAG CTTTGTTTGC CCTCACTCCT 
GAAGGTGGGG TCCTCAAGAA AGTCATCAGG CACAAGCGAG AGAGTGGGCT GAACTTGACC 
TTGCCAGAGG AGAATCAGCC AGTGGTGTTC AACCATATCT ACAACATCAA GTTGCCCATG 
GGTTCTCAGT GCTCAGTGGA TCTAGAGTCA ACGAGTGGAG AGAAAGACCT GAGCCCCACA 
CCAGAGTCCA GTGGAAGCTT CCAGGAGCAT ACAGTGGATG GGGAAAATCA GATTGTGTTC 
ACACACCGCA TCAACATCCC TCGTCGGGCC TGTGGCTGTG CCGCAGCTCC AGATGTGAAG 
GAGCTCCTGA GCAGACTGGA GGAACTGGAG ATGTTGGTAT CTTCTCTAAG GGAGCAGTGC 
ACCATGGGTA CAGGCTGTTG CCTCCAACCT GCAGAAGGCC GTCTGGACAG CAGGCCCTTC 
TGCAGCGGCA GGGGTAACTT CAGTGCTGAA GGTTGTGGCT GTGTCTGTGA ACCAGGCTGG 
AAAGGTCCCA ACTGCTCTGA GCCTGAATGC CCTGGAAACT GTAATCTCAG AGGCCAGTGC 
CTTGATGGAC AGTGTATCTG TGACCAGGGT TTCACTGGGG AAGACTGCAG CCAGCTAGCC 
TGTCCCAATG ACTGCAATGA CCAGGGCAAG TGTGTGAATG GGGTCTGTGT GTGCTTCGAA 
GGCTATGCCG GCCTTGACTG TGGCCTGGAA GTCTGCCCAG TGCCGTGCAG CGAGGAACAC 
GGGATGTGTG TGGATGGCAG GTGTGTGTGC AAAGATGGCT TTGCTGGTGA AGACTGCAAT 
GAGCCCCTTT GCTTCAACAA CTGCAACAAC CGTGGGCGGT GTGTGGAGAA CGAATGCGTC 
TGTGATGAGG GCTTCACGGG CGAAGACTGC AGCGAGCTCA TTTGCCCCAA TGACTGCTTC 
GACCGAGGTC GCTGCATCAA CGGCACCTGC TACTGTGAAG AAGGTTTCAC AGGTGAAGAC 
TGTGGTGAGC TCACCTGCCC CAACAACTGT CAGGGCCATG GCCAGTGTGA AGAGGGACAG 
TGTGTTTGCA ACGAGGGCTT TGCAGGGGCA GACTGCAGTG AAAAGCGGTG TCCCGCAGAC 
TGTCACCACC ATGGCCGCTG TCTCAACGGA CAGTGTGAGT GTGACGATGG GTTCACAGGG 
GCTGACTGTG GGGAACTCCA GTGCCCCAAT GGCTGCAGTG GGCATGGCCG CTGTGTCAAT 
GGGCAGTGTG TGTGTGACGA GGGCTACACC GGAGAAGACT GTAGCCAACG GCGATGCCCC 
AATGACTGCC ACAACCGGGG TCACTGTGTA CAGGGCAAAT GCATATGTGA GCAAGGCTTC 
AAAGGCTTTG ACTGTAGTGA GATGAGCTGT CCCAATGACT GCCACCAGCA TGGCCGCTGT 
GTGAATGGCA TGTGCATCTG TGATGATGAC TACACTGGGG AAGACTGCAG AGACCGCCGC 
TGTCCCCGGG ACTGTAGCCA GAGGGGGCGC TGTGTGGACG GACAGTGCAT TTGTGAGGAT 
GGTTTCACTG GTTCTGACTG TGCTGAGCTC TCCTGCCCTG GTGACTGCCA CGGCCATGGC 
CGCTGTGTGA ATGGCCAATG CATCTGCCAC GAGGGCTTCA CCGGCAAAGA CTGCAAAGAG 
CAAAGGTGCC CCAGTGACTG CCACGGCCAA GGCCGCTGTG AGGACGGCCA ATGTATCTGT 
CATGAGGGCT TCACAGGCCT GGACTGTGGG CAGCGCTCCT GTCCCAATGA CTGCAGCAAC 
CAGGGACAAT GTGTGGCAGG CCGCTGCATC TGCAATGAAG GCTACACAGG GGTAGACTGC 
TCTGAGGTGT CCCCTCCCAA AGACCTTATT GTGACAGAAG TAACAGAGGA GACTGTAAAT 
CTGGCATGGG ACAATGAGAT GCGGGTCACT GAGTACCTCA TTATGTACAC ACCCACCCAT 
GCTGATGGCC TGGAGATGCA GTTCCGTGTG CCTGGGGACC AGACATCTAC CACCATCCGG 
GAGCTGGAGC CAGGAGTGGA GTACTTCATT CATGTGTTCG CCATCTTGGA GAACAAGAGG 
AGCATCCCTG TCAGTGCCAG AGTTGCCACC TACTTGCCTG CACCTGAAGG CCTAAAATTC 
AAGTCTATCA AGGAGACATC TGTGGAAGTA GAGTGGGATC CTCTGGACAT CGCTTTTGAA 
ACGTGGGAGA TCATTTTCAG AAATATGAAC AAAGAAGATG AGGGAGAGAT CACAAAAAGC 
TTGAGGAGGC CAGAGACCTC CTACCGCCAA ACTGGCCTGG CTCCTGGCCA AGAATATGAA 
ATATCTCTTC ACATTGTGAA AAACAACACC CGAGGCCCTG GCTTGAAGAA AGTGACCACA 
ACTCGCCTGG ATGCCCCCAG CCAAATTGAG GTGAGAGACA TCACAGACAC TACAGCACTG 
ATCACCTGGT CCAAGCCCTT GGCTGAAATT GATAGCATTG AGCTCTCCTA TGGCATCAAG 
GATGTGCCTG GAGACCGGAC CACCATCGAC CTCACACATG AAGACAACCA GTACTCCATC 
GGGAACCTGA AACCTGACAC GGAGTATGAG GTGTCCCTCA TCTCCCGCAG AGTGGACATG 
GCAAGCAACC CTGCCAAGGA GACCTTCATC ACAGGCCTGG ATGCTCCCAG GAATCTCCGT 
CGCGTCTCAC AGACAGACAA CAGCATCACC TTGGAGTGGA GGAATGTCAA GGCAGACATT 
GATAGTTATA GAATTAAGTA TGCACCTATC TCTGGAGGTG ACCATGCTGA GATAGATGTT 
CCAAAGAGCC AGCAAGCCAC AACCAAAACC ACACTCACAG GTCTAAGGCC AGGAACTGAA 
TATGGAGTGG GCGTCTCTGC TGTTAAGGGA GACAAGGAGG GCGATCCAGC AACCATCAAT 
GCAGCCACAG AAATTGATGC ACCCAGGGAC TTACAGGTGT CTGAGACCAC ACAAGACAGT 
CTGACTTTAT TCTGGAAGAC ACCTCTGGCC AAGTTTGATC GTTACCGCCT CAACTACAGC 
CTCCCCACAG GCCAGTCAAT AGAGATTCAG CTGCCAAAGG ATGCCACCTC CCATGTCCTG 
ACAGACCTGG AGCCAGGGCA AGAATACACT GTTCTCCTCA CCGCTGAGAA GGGCAGACAT 
AAGAGCAAGC CTGCGCGTGT GAAGGCATCC ACAGAAGAAG TACCTTCTCT GGAAAATCTC 
ACCGTGACCG AGGCCGGCTG GGATGGCCTC AGACTCAACT GGACTGCAGA TGACCTGGCC 
TATGAGTACT TTGTTATTCA GGTACAGGAA GCCAACAAGG TGGAGACTGC TCACAACTTC 
ACAGTACTTG GTAACCTCCG GACTGCAGAC ATCCCGGGCC TCAAGGCTGC CACTCCTTAT 
AGAGTCTCCA TTTATGGGGT AGCTCGGGGC TATAAAACAC CCGTGCTCTC TGCTGAGACC 
TTCACAGGGA AAACTCCCAG TTTGGGAGAG GTGACTGTGG CTGAGGTGGG CTGGGATGCC 
CTCAAGCTCA ACTGGACTGC TCCAGAAGGA GCCTATACGA ACTTTTTCAT TCAGGTGCTA 
GAGGCTGACA TGACCCAGAC TGTCCAGAAC CTCACAGTCC CAGGGGGACT GAGGTCAGTG 
GACCTACCTG GGCTCAAAGC AGCCACCCGC TATTACATCA CCGTTCGAGG GGTCACCCAG 
GACTTCAGCA CAGCCCCTCT CTCTGTTGAG GTCTTGACAG AGGAGATCCC TCAACTGGGA 
GGCTTGTCCG TGACTGAGGT CAGCTGGGAC AGCCTTACAC TCAACTGGAC CACAGATGAT 
CTGGCCTATA AACACTTTAT CATTCAGGTG CAGGAGGCCA ACAATGTAGA GGCTGCTCAG 
AACCTCACAG TATCTGGTAG CCTCAGAGTT GTGGACATCC CAGGCCTCAA GGCTGATACC 
CCTTATAGAG TCTCCATCTA TGGGGTGATC CAGGGCTATA GAACACCAAT GCTCTCTGCT 
GATGTCTCCA CAGCCAAAGA ACCTGAAATT GGAAACTTAA ATATTTCTGA TGTAACTCCT 
GAGAGCTTCA ATCTCTCCTG GACAGCTACC GACGGGATCT TCGACATGTT TACTATCGAA 
ATTATTGATT CTAATAGGTT GCTGCAGACA GCAGAACATA ATATATCTGG TGCTGAACGA 
ACTGCCCACA TCTCGGGCCT TCCACCTAGT ACTGATTTCA TTGTCTACCT CTCTGGAATT 
GCTCCCAGCA TCCGTACCAA AACCATCAGT ACCACAGCCA CCACAGAAGC TGAACCGGAA 
GTTGACAACC TTCTAGTTTC AGATGCTACT CCAGACGGTT TCCGTCTGTC CTGGACTGCT 
GATGAAGGGA TATTCGACAG TTTTGTTATC AGGATCAGAG ATACCAAAAA GCAATCTGAA 
CCACAAGAAA TAACCCTCCC TTCCCCCGAC CGTACCAGGG ACATAACAGG TCTCAGAGAG 
GCCACTGAGT ACGAAATTGA ACTCTATGGA ATAAGCCGTG GAAGGCGATC CCAGCCAGTC 
AGTGCCATAG CAACAACAGC CATGGGTTCT CCGAAGGAAA TCATGTTCTC AGACATCACT 
GAAAATGCAG CCACAGTCAG CTGGAGAGCA CCGACTGCTC AAGTGGAGAG TTTCCGGATT 
ACTTATGTGC CTGTAACAGG AGGTCCCCCA TCCATGGTGA CCGTGGATGG GACGGATACT 
GAGACCCGAC TGGTGAGGCT CACCCCTGGA GTGGAGTACC ACGTCAGTGT GATTGCCATG 
AAGGGATTTG AAGAAAGTGA TCCGGTCTCG GGGTCTCTAA TCACAGCTCT CGATGGTCCA 
TCTGGCCTTC TGACAGCCAA CATCACAGAC TCAGAAGCCT TGGCCATGTG GCAGCCAGCC 
ATTGCCACTG TAGACAGTTA TGTCATCTCC TACACAGGGG AGAGAGTGCC AGAAATTACC 
CGCACAGTGT CTGGGAATAC AGTGGAGTTT GAACTACATG ACCTGGAGCC TGCCACAGAG 
TACACACTGA GTGTCTTTGC AGAGAAAGGT CACCAGAAGA GTTCTACCAT CGCTACGAAA 
TTTACCACAG ACCTGGATTC CCCAAGAGAA TTGACTGCTA CTGAGGTTCA GTCAGAAACT 
GCCCTCCTCA CCTGGAGACC TCCCCGGGCA TCGGTCACTG GATACCTCCT GGTCTATGAG 
TCTGTGGATG GTACAGTCAA GGAAGTCATT GTGGGGCCTG ACACCACCTC CTACAGCCTG 
GCAGACCTGA GCCCATCCAC CCACTACACA GCAAGAATCC AGGCATTGAG TGGGTCCCTG 
AGGAGCAAAC TGATCCAAAC CATCTTCACC ACAATTGGAC TCCTATATCC ATTCCCTAGG 
GATTGCTCTC AAGCAATGTT GAATGGCGAC ACCACCTCTG GCCTCTATAC CATCTATATA 
AATGGTGACA AGACTCAAGC ACTGGAAGTC TACTGTGATA TGACCTCTGA TGGAGGTGGA 
TGGATCGTTT TCCTGAGACG CAAAAATGGA CGTGAGGACT TCTATCGAAA CTGGAAGGCC 
TATGCCGCTG GGTTTGGAGA CCGCAGAGAA GAATTCTGGC TTGGACTGGA TAACCTGAGC 
AAAATCACAG CCCAAGGGCA ATATGAGCTC CGGGTGGACC TACAAGACCA TGGGGAGTCA 
GCCTATGCTG TGTATGACAG GTTCAGTGTT GGAGATGCCA AGAGTCGCTA CAAGCTGAAG 
GTAGAAGGAT ACAGTGGAAC GGCAGGTGAC TCCATGAACT ATCACAATGG TAGATCCTTC 
TCTACCTATG ACAAGGACAC AGACTCAGCC ATCACCAACT GTGCCCTGTC CTACAAAGGA 
GCTTTCTGGT ATAAGAACTG TCATCGTGTC AACCTGATGG GCAGATATGG GGACAATAAT 
CACAGTCAGG GTGTTAACTG GTTCCACTGG AAGGGCCATG AGTACTCAAT CCAGTTTGCG 
GAGATGAAAC TAAGACCCAG CAACTTCCGA AATCTGGAAG GCAGGCGTAA GCGGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761979.1
CDS	378..6437
Translation	MGAVTWLLPGIFLALFALTPEGGVLKKVIRHKRESGLNLTLPEENQPVVFNHIYNIKLPMGSQCSVDLESTSGEKDLSPTPESSGSFQEHTVDGENQIVFTHRINIPRRACGCAAAPDVKELLSRLEELEMLVSSLREQCTMGTGCCLQPAEGRLDSRPFCSGRGNFSAEGCGCVCEPGWKGPNCSEPECPGNCNLRGQCLDGQCICDQGFTGEDCSQLACPNDCNDQGKCVNGVCVCFEGYAGLDCGLEVCPVPCSEEHGMCVDGRCVCKDGFAGEDCNEPLCFNNCNNRGRCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGELTCPNNCQGHGQCEEGQCVCNEGFAGADCSEKRCPADCHHHGRCLNGQCECDDGFTGADCGELQCPNGCSGHGRCVNGQCVCDEGYTGEDCSQRRCPNDCHNRGHCVQGKCICEQGFKGFDCSEMSCPNDCHQHGRCVNGMCICDDDYTGEDCRDRRCPRDCSQRGRCVDGQCICEDGFTGSDCAELSCPGDCHGHGRCVNGQCICHEGFTGKDCKEQRCPSDCHGQGRCEDGQCICHEGFTGLDCGQRSCPNDCSNQGQCVAGRCICNEGYTGVDCSEVSPPKDLIVTEVTEETVNLAWDNEMRVTEYLIMYTPTHADGLEMQFRVPGDQTSTTIRELEPGVEYFIHVFAILENKRSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEGEITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKKVTTTRLDAPSQIEVRDITDTTALITWSKPLAEIDSIELSYGIKDVPGDRTTIDLTHEDNQYSIGNLKPDTEYEVSLISRRVDMASNPAKETFITGLDAPRNLRRVSQTDNSITLEWRNVKADIDSYRIKYAPISGGDHAEIDVPKSQQATTKTTLTGLRPGTEYGVGVSAVKGDKEGDPATINAATEIDAPRDLQVSETTQDSLTLFWKTPLAKFDRYRLNYSLPTGQSIEIQLPKDATSHVLTDLEPGQEYTVLLTAEKGRHKSKPARVKASTEEVPSLENLTVTEAGWDGLRLNWTADDLAYEYFVIQVQEANKVETAHNFTVLGNLRTADIPGLKAATPYRVSIYGVARGYKTPVLSAETFTGKTPSLGEVTVAEVGWDALKLNWTAPEGAYTNFFIQVLEADMTQTVQNLTVPGGLRSVDLPGLKAATRYYITVRGVTQDFSTAPLSVEVLTEEIPQLGGLSVTEVSWDSLTLNWTTDDLAYKHFIIQVQEANNVEAAQNLTVSGSLRVVDIPGLKADTPYRVSIYGVIQGYRTPMLSADVSTAKEPEIGNLNISDVTPESFNLSWTATDGIFDMFTIEIIDSNRLLQTAEHNISGAERTAHISGLPPSTDFIVYLSGIAPSIRTKTISTTATTEAEPEVDNLLVSDATPDGFRLSWTADEGIFDSFVIRIRDTKKQSEPQEITLPSPDRTRDITGLREATEYEIELYGISRGRRSQPVSAIATTAMGSPKEIMFSDITENAATVSWRAPTAQVESFRITYVPVTGGPPSMVTVDGTDTETRLVRLTPGVEYHVSVIAMKGFEESDPVSGSLITALDGPSGLLTANITDSEALAMWQPAIATVDSYVISYTGERVPEITRTVSGNTVEFELHDLEPATEYTLSVFAEKGHQKSSTIATKFTTDLDSPRELTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTARIQALSGSLRSKLIQTIFTTIGLLYPFPRDCSQAMLNGDTTSGLYTIYINGDKTQALEVYCDMTSDGGGWIVFLRRKNGREDFYRNWKAYAAGFGDRREEFWLGLDNLSKITAQGQYELRVDLQDHGESAYAVYDRFSVGDAKSRYKLKVEGYSGTAGDSMNYHNGRSFSTYDKDTDSAITNCALSYKGAFWYKNCHRVNLMGRYGDNNHSQGVNWFHWKGHEYSIQFAEMKLRPSNFRNLEGRRKRA

Target ORF information:

RefSeq Version	XM_008763757.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tenascin C (Tnc), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763757.2

ORF Insert Sequence:

ATGGGGGCCG TGACCTGGCT ATTGCCAGGC ATCTTCCTAG CTTTGTTTGC CCTCACTCCT 
GAAGGTGGGG TCCTCAAGAA AGTCATCAGG CACAAGCGAG AGAGTGGGCT GAACTTGACC 
TTGCCAGAGG AGAATCAGCC AGTGGTGTTC AACCATATCT ACAACATCAA GTTGCCCATG 
GGTTCTCAGT GCTCAGTGGA TCTAGAGTCA ACGAGTGGAG AGAAAGACCT GAGCCCCACA 
CCAGAGTCCA GTGGAAGCTT CCAGGAGCAT ACAGTGGATG GGGAAAATCA GATTGTGTTC 
ACACACCGCA TCAACATCCC TCGTCGGGCC TGTGGCTGTG CCGCAGCTCC AGATGTGAAG 
GAGCTCCTGA GCAGACTGGA GGAACTGGAG ATGTTGGTAT CTTCTCTAAG GGAGCAGTGC 
ACCATGGGTA CAGGCTGTTG CCTCCAACCT GCAGAAGGCC GTCTGGACAG CAGGCCCTTC 
TGCAGCGGCA GGGGTAACTT CAGTGCTGAA GGTTGTGGCT GTGTCTGTGA ACCAGGCTGG 
AAAGGTCCCA ACTGCTCTGA GCCTGAATGC CCTGGAAACT GTAATCTCAG AGGCCAGTGC 
CTTGATGGAC AGTGTATCTG TGACCAGGGT TTCACTGGGG AAGACTGCAG CCAGCTAGCC 
TGTCCCAATG ACTGCAATGA CCAGGGCAAG TGTGTGAATG GGGTCTGTGT GTGCTTCGAA 
GGCTATGCCG GCCTTGACTG TGGCCTGGAA GTCTGCCCAG TGCCGTGCAG CGAGGAACAC 
GGGATGTGTG TGGATGGCAG GTGTGTGTGC AAAGATGGCT TTGCTGGTGA AGACTGCAAT 
GAGCCCCTTT GCTTCAACAA CTGCAACAAC CGTGGGCGGT GTGTGGAGAA CGAATGCGTC 
TGTGATGAGG GCTTCACGGG CGAAGACTGC AGCGAGCTCA TTTGCCCCAA TGACTGCTTC 
GACCGAGGTC GCTGCATCAA CGGCACCTGC TACTGTGAAG AAGGTTTCAC AGGTGAAGAC 
TGTGGTGAGC TCACCTGCCC CAACAACTGT CAGGGCCATG GCCAGTGTGA AGAGGGACAG 
TGTGTTTGCA ACGAGGGCTT TGCAGGGGCA GACTGCAGTG AAAAGCGGTG TCCCGCAGAC 
TGTCACCACC ATGGCCGCTG TCTCAACGGA CAGTGTGAGT GTGACGATGG GTTCACAGGG 
GCTGACTGTG GGGAACTCCA GTGCCCCAAT GGCTGCAGTG GGCATGGCCG CTGTGTCAAT 
GGGCAGTGTG TGTGTGACGA GGGCTACACC GGAGAAGACT GTAGCCAACG GCGATGCCCC 
AATGACTGCC ACAACCGGGG TCACTGTGTA CAGGGCAAAT GCATATGTGA GCAAGGCTTC 
AAAGGCTTTG ACTGTAGTGA GATGAGCTGT CCCAATGACT GCCACCAGCA TGGCCGCTGT 
GTGAATGGCA TGTGCATCTG TGATGATGAC TACACTGGGG AAGACTGCAG AGACCGCCGC 
TGTCCCCGGG ACTGTAGCCA GAGGGGGCGC TGTGTGGACG GACAGTGCAT TTGTGAGGAT 
GGTTTCACTG GTTCTGACTG TGCTGAGCTC TCCTGCCCTG GTGACTGCCA CGGCCATGGC 
CGCTGTGTGA ATGGCCAATG CATCTGCCAC GAGGGCTTCA CCGGCAAAGA CTGCAAAGAG 
CAAAGGTGCC CCAGTGACTG CCACGGCCAA GGCCGCTGTG AGGACGGCCA ATGTATCTGT 
CATGAGGGCT TCACAGGCCT GGACTGTGGG CAGCGCTCCT GTCCCAATGA CTGCAGCAAC 
CAGGGACAAT GTGTGGCAGG CCGCTGCATC TGCAATGAAG GCTACACAGG GGTAGACTGC 
TCTGAGGTGT CCCCTCCCAA AGACCTTATT GTGACAGAAG TAACAGAGGA GACTGTAAAT 
CTGGCATGGG ACAATGAGAT GCGGGTCACT GAGTACCTCA TTATGTACAC ACCCACCCAT 
GCTGATGGCC TGGAGATGCA GTTCCGTGTG CCTGGGGACC AGACATCTAC CACCATCCGG 
GAGCTGGAGC CAGGAGTGGA GTACTTCATT CATGTGTTCG CCATCTTGGA GAACAAGAGG 
AGCATCCCTG TCAGTGCCAG AGTTGCCACC TACTTGCCTG CACCTGAAGG CCTAAAATTC 
AAGTCTATCA AGGAGACATC TGTGGAAGTA GAGTGGGATC CTCTGGACAT CGCTTTTGAA 
ACGTGGGAGA TCATTTTCAG AAATATGAAC AAAGAAGATG AGGGAGAGAT CACAAAAAGC 
TTGAGGAGGC CAGAGACCTC CTACCGCCAA ACTGGCCTGG CTCCTGGCCA AGAATATGAA 
ATATCTCTTC ACATTGTGAA AAACAACACC CGAGGCCCTG GCTTGAAGAA AGTGACCACA 
ACTCGCCTGG ATGCCCCCAG CCAAATTGAG GTGAGAGACA TCACAGACAC TACAGCACTG 
ATCACCTGGT CCAAGCCCTT GGCTGAAATT GATAGCATTG AGCTCTCCTA TGGCATCAAG 
GATGTGCCTG GAGACCGGAC CACCATCGAC CTCACACATG AAGACAACCA GTACTCCATC 
GGGAACCTGA AACCTGACAC GGAGTATGAG GTGTCCCTCA TCTCCCGCAG AGTGGACATG 
GCAAGCAACC CTGCCAAGGA GACCTTCATC ACAGGCCTGG ATGCTCCCAG GAATCTCCGT 
CGCGTCTCAC AGACAGACAA CAGCATCACC TTGGAGTGGA GGAATGTCAA GGCAGACATT 
GATAGTTATA GAATTAAGTA TGCACCTATC TCTGGAGGTG ACCATGCTGA GATAGATGTT 
CCAAAGAGCC AGCAAGCCAC AACCAAAACC ACACTCACAG GTCTAAGGCC AGGAACTGAA 
TATGGAGTGG GCGTCTCTGC TGTTAAGGGA GACAAGGAGG GCGATCCAGC AACCATCAAT 
GCAGCCACAG AAATTGATGC ACCCAGGGAC TTACAGGTGT CTGAGACCAC ACAAGACAGT 
CTGACTTTAT TCTGGAAGAC ACCTCTGGCC AAGTTTGATC GTTACCGCCT CAACTACAGC 
CTCCCCACAG GCCAGTCAAT AGAGATTCAG CTGCCAAAGG ATGCCACCTC CCATGTCCTG 
ACAGACCTGG AGCCAGGGCA AGAATACACT GTTCTCCTCA CCGCTGAGAA GGGCAGACAT 
AAGAGCAAGC CTGCGCGTGT GAAGGCATCC ACAGAAGAAG TACCTTCTCT GGAAAATCTC 
ACCGTGACCG AGGCCGGCTG GGATGGCCTC AGACTCAACT GGACTGCAGA TGACCTGGCC 
TATGAGTACT TTGTTATTCA GGTACAGGAA GCCAACAAGG TGGAGACTGC TCACAACTTC 
ACAGTACTTG GTAACCTCCG GACTGCAGAC ATCCCGGGCC TCAAGGCTGC CACTCCTTAT 
AGAGTCTCCA TTTATGGGGT AGCTCGGGGC TATAAAACAC CCGTGCTCTC TGCTGAGACC 
TTCACAGGGA AAACTCCCAG TTTGGGAGAG GTGACTGTGG CTGAGGTGGG CTGGGATGCC 
CTCAAGCTCA ACTGGACTGC TCCAGAAGGA GCCTATACGA ACTTTTTCAT TCAGGTGCTA 
GAGGCTGACA TGACCCAGAC TGTCCAGAAC CTCACAGTCC CAGGGGGACT GAGGTCAGTG 
GACCTACCTG GGCTCAAAGC AGCCACCCGC TATTACATCA CCGTTCGAGG GGTCACCCAG 
GACTTCAGCA CAGCCCCTCT CTCTGTTGAG GTCTTGACAG AGGAGATCCC TCAACTGGGA 
GGCTTGTCCG TGACTGAGGT CAGCTGGGAC AGCCTTACAC TCAACTGGAC CACAGATGAT 
CTGGCCTATA AACACTTTAT CATTCAGGTG CAGGAGGCCA ACAATGTAGA GGCTGCTCAG 
AACCTCACAG TATCTGGTAG CCTCAGAGTT GTGGACATCC CAGGCCTCAA GGCTGATACC 
CCTTATAGAG TCTCCATCTA TGGGGTGATC CAGGGCTATA GAACACCAAT GCTCTCTGCT 
GATGTCTCCA CAGCCAAAGA ACCTGAAATT GGAAACTTAA ATATTTCTGA TGTAACTCCT 
GAGAGCTTCA ATCTCTCCTG GACAGCTACC GACGGGATCT TCGACATGTT TACTATCGAA 
ATTATTGATT CTAATAGGTT GCTGCAGACA GCAGAACATA ATATATCTGG TGCTGAACGA 
ACTGCCCACA TCTCGGGCCT TCCACCTAGT ACTGATTTCA TTGTCTACCT CTCTGGAATT 
GCTCCCAGCA TCCGTACCAA AACCATCAGT ACCACAGCCA CCACAGAAGC TGAACCGGAA 
GTTGACAACC TTCTAGTTTC AGATGCTACT CCAGACGGTT TCCGTCTGTC CTGGACTGCT 
GATGAAGGGA TATTCGACAG TTTTGTTATC AGGATCAGAG ATACCAAAAA GCAATCTGAA 
CCACAAGAAA TAACCCTCCC TTCCCCCGAC CGTACCAGGG ACATAACAGG TCTCAGAGAG 
GCCACTGAGT ACGAAATTGA ACTCTATGGA ATAAGCCGTG GAAGGCGATC CCAGCCAGTC 
AGTGCCATAG CAACAACAGC CATGGGTTCT CCGAAGGAAA TCATGTTCTC AGACATCACT 
GAAAATGCAG CCACAGTCAG CTGGAGAGCA CCGACTGCTC AAGTGGAGAG TTTCCGGATT 
ACTTATGTGC CTGTAACAGG AGGTCCCCCA TCCATGGTGA CCGTGGATGG GACGGATACT 
GAGACCCGAC TGGTGAGGCT CACCCCTGGA GTGGAGTACC ACGTCAGTGT GATTGCCATG 
AAGGGATTTG AAGAAAGTGA TCCGGTCTCG GGGTCTCTAA TCACAGCTCT CGATGGTCCA 
TCTGGCCTTC TGACAGCCAA CATCACAGAC TCAGAAGCCT TGGCCATGTG GCAGCCAGCC 
ATTGCCACTG TAGACAGTTA TGTCATCTCC TACACAGGGG AGAGAGTGCC AGAAATTACC 
CGCACAGTGT CTGGGAATAC AGTGGAGTTT GAACTACATG ACCTGGAGCC TGCCACAGAG 
TACACACTGA GTGTCTTTGC AGAGAAAGGT CACCAGAAGA GTTCTACCAT CGCTACGAAA 
TTTACCACAG ACCTGGATTC CCCAAGAGAA TTGACTGCTA CTGAGGTTCA GTCAGAAACT 
GCCCTCCTCA CCTGGAGACC TCCCCGGGCA TCGGTCACTG GATACCTCCT GGTCTATGAG 
TCTGTGGATG GTACAGTCAA GGAAGTCATT GTGGGGCCTG ACACCACCTC CTACAGCCTG 
GCAGACCTGA GCCCATCCAC CCACTACACA GCAAGAATCC AGGCATTGAG TGGGTCCCTG 
AGGAGCAAAC TGATCCAAAC CATCTTCACC ACAATTGGAC TCCTATATCC ATTCCCTAGG 
GATTGCTCTC AAGCAATGTT GAATGGCGAC ACCACCTCTG GCCTCTATAC CATCTATATA 
AATGGTGACA AGACTCAAGC ACTGGAAGTC TACTGTGATA TGACCTCTGA TGGAGGTGGA 
TGGATCGTTT TCCTGAGACG CAAAAATGGA CGTGAGGACT TCTATCGAAA CTGGAAGGCC 
TATGCCGCTG GGTTTGGAGA CCGCAGAGAA GAATTCTGGC TTGGACTGGA TAACCTGAGC 
AAAATCACAG CCCAAGGGCA ATATGAGCTC CGGGTGGACC TACAAGACCA TGGGGAGTCA 
GCCTATGCTG TGTATGACAG GTTCAGTGTT GGAGATGCCA AGAGTCGCTA CAAGCTGAAG 
GTAGAAGGAT ACAGTGGAAC GGCAGGTGAC TCCATGAACT ATCACAATGG TAGATCCTTC 
TCTACCTATG ACAAGGACAC AGACTCAGCC ATCACCAACT GTGCCCTGTC CTACAAAGGA 
GCTTTCTGGT ATAAGAACTG TCATCGTGTC AACCTGATGG GCAGATATGG GGACAATAAT 
CACAGTCAGG GTGTTAACTG GTTCCACTGG AAGGGCCATG AGTACTCAAT CCAGTTTGCG 
GAGATGAAAC TAAGACCCAG CAACTTCCGA AATCTGGAAG GCAGGCGTAA GCGGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnc ( XM_008763758.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761980.1 Rattus norvegicus tenascin C (Tnc), transcript variant X5, mRNA.

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CloneID	ORa44024
Clone ID Related Accession (Same CDS sequence)	XM_008763758.2
Accession Version	XM_008763758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tenascin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCCG TGACCTGGCT ATTGCCAGGC ATCTTCCTAG CTTTGTTTGC CCTCACTCCT 
GAAGGTGGGG TCCTCAAGAA AGTCATCAGG CACAAGCGAG AGAGTGGGCT GAACTTGACC 
TTGCCAGAGG AGAATCAGCC AGTGGTGTTC AACCATATCT ACAACATCAA GTTGCCCATG 
GGTTCTCAGT GCTCAGTGGA TCTAGAGTCA ACGAGTGGAG AGAAAGACCT GAGCCCCACA 
CCAGAGTCCA GTGGAAGCTT CCAGGAGCAT ACAGTGGATG GGGAAAATCA GATTGTGTTC 
ACACACCGCA TCAACATCCC TCGTCGGGCC TGTGGCTGTG CCGCAGCTCC AGATGTGAAG 
GAGCTCCTGA GCAGACTGGA GGAACTGGAG ATGTTGGTAT CTTCTCTAAG GGAGCAGTGC 
ACCATGGGTA CAGGCTGTTG CCTCCAACCT GCAGAAGGCC GTCTGGACAG CAGGCCCTTC 
TGCAGCGGCA GGGGTAACTT CAGTGCTGAA GGTTGTGGCT GTGTCTGTGA ACCAGGCTGG 
AAAGGTCCCA ACTGCTCTGA GCCTGAATGC CCTGGAAACT GTAATCTCAG AGGCCAGTGC 
CTTGATGGAC AGTGTATCTG TGACCAGGGT TTCACTGGGG AAGACTGCAG CCAGCTAGCC 
TGTCCCAATG ACTGCAATGA CCAGGGCAAG TGTGTGAATG GGGTCTGTGT GTGCTTCGAA 
GGCTATGCCG GCCTTGACTG TGGCCTGGAA GTCTGCCCAG TGCCGTGCAG CGAGGAACAC 
GGGATGTGTG TGGATGGCAG GTGTGTGTGC AAAGATGGCT TTGCTGGTGA AGACTGCAAT 
GAGCCCCTTT GCTTCAACAA CTGCAACAAC CGTGGGCGGT GTGTGGAGAA CGAATGCGTC 
TGTGATGAGG GCTTCACGGG CGAAGACTGC AGCGAGCTCA TTTGCCCCAA TGACTGCTTC 
GACCGAGGTC GCTGCATCAA CGGCACCTGC TACTGTGAAG AAGGTTTCAC AGGTGAAGAC 
TGTGGTGAGC TCACCTGCCC CAACAACTGT CAGGGCCATG GCCAGTGTGA AGAGGGACAG 
TGTGTTTGCA ACGAGGGCTT TGCAGGGGCA GACTGCAGTG AAAAGCGGTG TCCCGCAGAC 
TGTCACCACC ATGGCCGCTG TCTCAACGGA CAGTGTGAGT GTGACGATGG GTTCACAGGG 
GCTGACTGTG GGGAACTCCA GTGCCCCAAT GGCTGCAGTG GGCATGGCCG CTGTGTCAAT 
GGGCAGTGTG TGTGTGACGA GGGCTACACC GGAGAAGACT GTAGCCAACG GCGATGCCCC 
AATGACTGCC ACAACCGGGG TCACTGTGTA CAGGGCAAAT GCATATGTGA GCAAGGCTTC 
AAAGGCTTTG ACTGTAGTGA GATGAGCTGT CCCAATGACT GCCACCAGCA TGGCCGCTGT 
GTGAATGGCA TGTGCATCTG TGATGATGAC TACACTGGGG AAGACTGCAG AGACCGCCGC 
TGTCCCCGGG ACTGTAGCCA GAGGGGGCGC TGTGTGGACG GACAGTGCAT TTGTGAGGAT 
GGTTTCACTG GTTCTGACTG TGCTGAGCTC TCCTGCCCTG GTGACTGCCA CGGCCATGGC 
CGCTGTGTGA ATGGCCAATG CATCTGCCAC GAGGGCTTCA CCGGCAAAGA CTGCAAAGAG 
CAAAGGTGCC CCAGTGACTG CCACGGCCAA GGCCGCTGTG AGGACGGCCA ATGTATCTGT 
CATGAGGGCT TCACAGGCCT GGACTGTGGG CAGCGCTCCT GTCCCAATGA CTGCAGCAAC 
CAGGGACAAT GTGTGGCAGG CCGCTGCATC TGCAATGAAG GCTACACAGG GGTAGACTGC 
TCTGAGGTGT CCCCTCCCAA AGACCTTATT GTGACAGAAG TAACAGAGGA GACTGTAAAT 
CTGGCATGGG ACAATGAGAT GCGGGTCACT GAGTACCTCA TTATGTACAC ACCCACCCAT 
GCTGATGGCC TGGAGATGCA GTTCCGTGTG CCTGGGGACC AGACATCTAC CACCATCCGG 
GAGCTGGAGC CAGGAGTGGA GTACTTCATT CATGTGTTCG CCATCTTGGA GAACAAGAGG 
AGCATCCCTG TCAGTGCCAG AGTTGCCACC TACTTGCCTG CACCTGAAGG CCTAAAATTC 
AAGTCTATCA AGGAGACATC TGTGGAAGTA GAGTGGGATC CTCTGGACAT CGCTTTTGAA 
ACGTGGGAGA TCATTTTCAG AAATATGAAC AAAGAAGATG AGGGAGAGAT CACAAAAAGC 
TTGAGGAGGC CAGAGACCTC CTACCGCCAA ACTGGCCTGG CTCCTGGCCA AGAATATGAA 
ATATCTCTTC ACATTGTGAA AAACAACACC CGAGGCCCTG GCTTGAAGAA AGTGACCACA 
ACTCGCCTGG ATGCCCCCAG CCAAATTGAG GTGAGAGACA TCACAGACAC TACAGCACTG 
ATCACCTGGT CCAAGCCCTT GGCTGAAATT GATAGCATTG AGCTCTCCTA TGGCATCAAG 
GATGTGCCTG GAGACCGGAC CACCATCGAC CTCACACATG AAGACAACCA GTACTCCATC 
GGGAACCTGA AACCTGACAC GGAGTATGAG GTGTCCCTCA TCTCCCGCAG AGTGGACATG 
GCAAGCAACC CTGCCAAGGA GACCTTCATC ACAGGCCTGG ATGCTCCCAG GAATCTCCGT 
CGCGTCTCAC AGACAGACAA CAGCATCACC TTGGAGTGGA GGAATGTCAA GGCAGACATT 
GATAGTTATA GAATTAAGTA TGCACCTATC TCTGGAGGTG ACCATGCTGA GATAGATGTT 
CCAAAGAGCC AGCAAGCCAC AACCAAAACC ACACTCACAG GTCTAAGGCC AGGAACTGAA 
TATGGAGTGG GCGTCTCTGC TGTTAAGGGA GACAAGGAGG GCGATCCAGC AACCATCAAT 
GCAGCCACAG AAATTGATGC ACCCAGGGAC TTACAGGTGT CTGAGACCAC ACAAGACAGT 
CTGACTTTAT TCTGGAAGAC ACCTCTGGCC AAGTTTGATC GTTACCGCCT CAACTACAGC 
CTCCCCACAG GCCAGTCAAT AGAGATTCAG CTGCCAAAGG ATGCCACCTC CCATGTCCTG 
ACAGACCTGG AGCCAGGGCA AGAATACACT GTTCTCCTCA CCGCTGAGAA GGGCAGACAT 
AAGAGCAAGC CTGCGCGTGT GAAGGCATCC ACAGAAGCTG AACCGGAAGT TGACAACCTT 
CTAGTTTCAG ATGCTACTCC AGACGGTTTC CGTCTGTCCT GGACTGCTGA TGAAGGGATA 
TTCGACAGTT TTGTTATCAG GATCAGAGAT ACCAAAAAGC AATCTGAACC ACAAGAAATA 
ACCCTCCCTT CCCCCGACCG TACCAGGGAC ATAACAGGTC TCAGAGAGGC CACTGAGTAC 
GAAATTGAAC TCTATGGAAT AAGCCGTGGA AGGCGATCCC AGCCAGTCAG TGCCATAGCA 
ACAACAGCCA TGGGTTCTCC GAAGGAAATC ATGTTCTCAG ACATCACTGA AAATGCAGCC 
ACAGTCAGCT GGAGAGCACC GACTGCTCAA GTGGAGAGTT TCCGGATTAC TTATGTGCCT 
GTAACAGGAG GTCCCCCATC CATGGTGACC GTGGATGGGA CGGATACTGA GACCCGACTG 
GTGAGGCTCA CCCCTGGAGT GGAGTACCAC GTCAGTGTGA TTGCCATGAA GGGATTTGAA 
GAAAGTGATC CGGTCTCGGG GTCTCTAATC ACAGCTCTCG ATGGTCCATC TGGCCTTCTG 
ACAGCCAACA TCACAGACTC AGAAGCCTTG GCCATGTGGC AGCCAGCCAT TGCCACTGTA 
GACAGTTATG TCATCTCCTA CACAGGGGAG AGAGTGCCAG AAATTACCCG CACAGTGTCT 
GGGAATACAG TGGAGTTTGA ACTACATGAC CTGGAGCCTG CCACAGAGTA CACACTGAGT 
GTCTTTGCAG AGAAAGGTCA CCAGAAGAGT TCTACCATCG CTACGAAATT TACCACAGAC 
CTGGATTCCC CAAGAGAATT GACTGCTACT GAGGTTCAGT CAGAAACTGC CCTCCTCACC 
TGGAGACCTC CCCGGGCATC GGTCACTGGA TACCTCCTGG TCTATGAGTC TGTGGATGGT 
ACAGTCAAGG AAGTCATTGT GGGGCCTGAC ACCACCTCCT ACAGCCTGGC AGACCTGAGC 
CCATCCACCC ACTACACAGC AAGAATCCAG GCATTGAGTG GGTCCCTGAG GAGCAAACTG 
ATCCAAACCA TCTTCACCAC AATTGGACTC CTATATCCAT TCCCTAGGGA TTGCTCTCAA 
GCAATGTTGA ATGGCGACAC CACCTCTGGC CTCTATACCA TCTATATAAA TGGTGACAAG 
ACTCAAGCAC TGGAAGTCTA CTGTGATATG ACCTCTGATG GAGGTGGATG GATCGTTTTC 
CTGAGACGCA AAAATGGACG TGAGGACTTC TATCGAAACT GGAAGGCCTA TGCCGCTGGG 
TTTGGAGACC GCAGAGAAGA ATTCTGGCTT GGACTGGATA ACCTGAGCAA AATCACAGCC 
CAAGGGCAAT ATGAGCTCCG GGTGGACCTA CAAGACCATG GGGAGTCAGC CTATGCTGTG 
TATGACAGGT TCAGTGTTGG AGATGCCAAG AGTCGCTACA AGCTGAAGGT AGAAGGATAC 
AGTGGAACGG CAGGTGACTC CATGAACTAT CACAATGGTA GATCCTTCTC TACCTATGAC 
AAGGACACAG ACTCAGCCAT CACCAACTGT GCCCTGTCCT ACAAAGGAGC TTTCTGGTAT 
AAGAACTGTC ATCGTGTCAA CCTGATGGGC AGATATGGGG ACAATAATCA CAGTCAGGGT 
GTTAACTGGT TCCACTGGAA GGGCCATGAG TACTCAATCC AGTTTGCGGA GATGAAACTA 
AGACCCAGCA ACTTCCGAAA TCTGGAAGGC AGGCGTAAGC GGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761980.1
CDS	374..5341
Translation	MGAVTWLLPGIFLALFALTPEGGVLKKVIRHKRESGLNLTLPEENQPVVFNHIYNIKLPMGSQCSVDLESTSGEKDLSPTPESSGSFQEHTVDGENQIVFTHRINIPRRACGCAAAPDVKELLSRLEELEMLVSSLREQCTMGTGCCLQPAEGRLDSRPFCSGRGNFSAEGCGCVCEPGWKGPNCSEPECPGNCNLRGQCLDGQCICDQGFTGEDCSQLACPNDCNDQGKCVNGVCVCFEGYAGLDCGLEVCPVPCSEEHGMCVDGRCVCKDGFAGEDCNEPLCFNNCNNRGRCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGELTCPNNCQGHGQCEEGQCVCNEGFAGADCSEKRCPADCHHHGRCLNGQCECDDGFTGADCGELQCPNGCSGHGRCVNGQCVCDEGYTGEDCSQRRCPNDCHNRGHCVQGKCICEQGFKGFDCSEMSCPNDCHQHGRCVNGMCICDDDYTGEDCRDRRCPRDCSQRGRCVDGQCICEDGFTGSDCAELSCPGDCHGHGRCVNGQCICHEGFTGKDCKEQRCPSDCHGQGRCEDGQCICHEGFTGLDCGQRSCPNDCSNQGQCVAGRCICNEGYTGVDCSEVSPPKDLIVTEVTEETVNLAWDNEMRVTEYLIMYTPTHADGLEMQFRVPGDQTSTTIRELEPGVEYFIHVFAILENKRSIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEGEITKSLRRPETSYRQTGLAPGQEYEISLHIVKNNTRGPGLKKVTTTRLDAPSQIEVRDITDTTALITWSKPLAEIDSIELSYGIKDVPGDRTTIDLTHEDNQYSIGNLKPDTEYEVSLISRRVDMASNPAKETFITGLDAPRNLRRVSQTDNSITLEWRNVKADIDSYRIKYAPISGGDHAEIDVPKSQQATTKTTLTGLRPGTEYGVGVSAVKGDKEGDPATINAATEIDAPRDLQVSETTQDSLTLFWKTPLAKFDRYRLNYSLPTGQSIEIQLPKDATSHVLTDLEPGQEYTVLLTAEKGRHKSKPARVKASTEAEPEVDNLLVSDATPDGFRLSWTADEGIFDSFVIRIRDTKKQSEPQEITLPSPDRTRDITGLREATEYEIELYGISRGRRSQPVSAIATTAMGSPKEIMFSDITENAATVSWRAPTAQVESFRITYVPVTGGPPSMVTVDGTDTETRLVRLTPGVEYHVSVIAMKGFEESDPVSGSLITALDGPSGLLTANITDSEALAMWQPAIATVDSYVISYTGERVPEITRTVSGNTVEFELHDLEPATEYTLSVFAEKGHQKSSTIATKFTTDLDSPRELTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTARIQALSGSLRSKLIQTIFTTIGLLYPFPRDCSQAMLNGDTTSGLYTIYINGDKTQALEVYCDMTSDGGGWIVFLRRKNGREDFYRNWKAYAAGFGDRREEFWLGLDNLSKITAQGQYELRVDLQDHGESAYAVYDRFSVGDAKSRYKLKVEGYSGTAGDSMNYHNGRSFSTYDKDTDSAITNCALSYKGAFWYKNCHRVNLMGRYGDNNHSQGVNWFHWKGHEYSIQFAEMKLRPSNFRNLEGRRKRA

Target ORF information:

RefSeq Version	XM_008763758.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tenascin C (Tnc), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763758.2

ORF Insert Sequence:

ATGGGGGCCG TGACCTGGCT ATTGCCAGGC ATCTTCCTAG CTTTGTTTGC CCTCACTCCT 
GAAGGTGGGG TCCTCAAGAA AGTCATCAGG CACAAGCGAG AGAGTGGGCT GAACTTGACC 
TTGCCAGAGG AGAATCAGCC AGTGGTGTTC AACCATATCT ACAACATCAA GTTGCCCATG 
GGTTCTCAGT GCTCAGTGGA TCTAGAGTCA ACGAGTGGAG AGAAAGACCT GAGCCCCACA 
CCAGAGTCCA GTGGAAGCTT CCAGGAGCAT ACAGTGGATG GGGAAAATCA GATTGTGTTC 
ACACACCGCA TCAACATCCC TCGTCGGGCC TGTGGCTGTG CCGCAGCTCC AGATGTGAAG 
GAGCTCCTGA GCAGACTGGA GGAACTGGAG ATGTTGGTAT CTTCTCTAAG GGAGCAGTGC 
ACCATGGGTA CAGGCTGTTG CCTCCAACCT GCAGAAGGCC GTCTGGACAG CAGGCCCTTC 
TGCAGCGGCA GGGGTAACTT CAGTGCTGAA GGTTGTGGCT GTGTCTGTGA ACCAGGCTGG 
AAAGGTCCCA ACTGCTCTGA GCCTGAATGC CCTGGAAACT GTAATCTCAG AGGCCAGTGC 
CTTGATGGAC AGTGTATCTG TGACCAGGGT TTCACTGGGG AAGACTGCAG CCAGCTAGCC 
TGTCCCAATG ACTGCAATGA CCAGGGCAAG TGTGTGAATG GGGTCTGTGT GTGCTTCGAA 
GGCTATGCCG GCCTTGACTG TGGCCTGGAA GTCTGCCCAG TGCCGTGCAG CGAGGAACAC 
GGGATGTGTG TGGATGGCAG GTGTGTGTGC AAAGATGGCT TTGCTGGTGA AGACTGCAAT 
GAGCCCCTTT GCTTCAACAA CTGCAACAAC CGTGGGCGGT GTGTGGAGAA CGAATGCGTC 
TGTGATGAGG GCTTCACGGG CGAAGACTGC AGCGAGCTCA TTTGCCCCAA TGACTGCTTC 
GACCGAGGTC GCTGCATCAA CGGCACCTGC TACTGTGAAG AAGGTTTCAC AGGTGAAGAC 
TGTGGTGAGC TCACCTGCCC CAACAACTGT CAGGGCCATG GCCAGTGTGA AGAGGGACAG 
TGTGTTTGCA ACGAGGGCTT TGCAGGGGCA GACTGCAGTG AAAAGCGGTG TCCCGCAGAC 
TGTCACCACC ATGGCCGCTG TCTCAACGGA CAGTGTGAGT GTGACGATGG GTTCACAGGG 
GCTGACTGTG GGGAACTCCA GTGCCCCAAT GGCTGCAGTG GGCATGGCCG CTGTGTCAAT 
GGGCAGTGTG TGTGTGACGA GGGCTACACC GGAGAAGACT GTAGCCAACG GCGATGCCCC 
AATGACTGCC ACAACCGGGG TCACTGTGTA CAGGGCAAAT GCATATGTGA GCAAGGCTTC 
AAAGGCTTTG ACTGTAGTGA GATGAGCTGT CCCAATGACT GCCACCAGCA TGGCCGCTGT 
GTGAATGGCA TGTGCATCTG TGATGATGAC TACACTGGGG AAGACTGCAG AGACCGCCGC 
TGTCCCCGGG ACTGTAGCCA GAGGGGGCGC TGTGTGGACG GACAGTGCAT TTGTGAGGAT 
GGTTTCACTG GTTCTGACTG TGCTGAGCTC TCCTGCCCTG GTGACTGCCA CGGCCATGGC 
CGCTGTGTGA ATGGCCAATG CATCTGCCAC GAGGGCTTCA CCGGCAAAGA CTGCAAAGAG 
CAAAGGTGCC CCAGTGACTG CCACGGCCAA GGCCGCTGTG AGGACGGCCA ATGTATCTGT 
CATGAGGGCT TCACAGGCCT GGACTGTGGG CAGCGCTCCT GTCCCAATGA CTGCAGCAAC 
CAGGGACAAT GTGTGGCAGG CCGCTGCATC TGCAATGAAG GCTACACAGG GGTAGACTGC 
TCTGAGGTGT CCCCTCCCAA AGACCTTATT GTGACAGAAG TAACAGAGGA GACTGTAAAT 
CTGGCATGGG ACAATGAGAT GCGGGTCACT GAGTACCTCA TTATGTACAC ACCCACCCAT 
GCTGATGGCC TGGAGATGCA GTTCCGTGTG CCTGGGGACC AGACATCTAC CACCATCCGG 
GAGCTGGAGC CAGGAGTGGA GTACTTCATT CATGTGTTCG CCATCTTGGA GAACAAGAGG 
AGCATCCCTG TCAGTGCCAG AGTTGCCACC TACTTGCCTG CACCTGAAGG CCTAAAATTC 
AAGTCTATCA AGGAGACATC TGTGGAAGTA GAGTGGGATC CTCTGGACAT CGCTTTTGAA 
ACGTGGGAGA TCATTTTCAG AAATATGAAC AAAGAAGATG AGGGAGAGAT CACAAAAAGC 
TTGAGGAGGC CAGAGACCTC CTACCGCCAA ACTGGCCTGG CTCCTGGCCA AGAATATGAA 
ATATCTCTTC ACATTGTGAA AAACAACACC CGAGGCCCTG GCTTGAAGAA AGTGACCACA 
ACTCGCCTGG ATGCCCCCAG CCAAATTGAG GTGAGAGACA TCACAGACAC TACAGCACTG 
ATCACCTGGT CCAAGCCCTT GGCTGAAATT GATAGCATTG AGCTCTCCTA TGGCATCAAG 
GATGTGCCTG GAGACCGGAC CACCATCGAC CTCACACATG AAGACAACCA GTACTCCATC 
GGGAACCTGA AACCTGACAC GGAGTATGAG GTGTCCCTCA TCTCCCGCAG AGTGGACATG 
GCAAGCAACC CTGCCAAGGA GACCTTCATC ACAGGCCTGG ATGCTCCCAG GAATCTCCGT 
CGCGTCTCAC AGACAGACAA CAGCATCACC TTGGAGTGGA GGAATGTCAA GGCAGACATT 
GATAGTTATA GAATTAAGTA TGCACCTATC TCTGGAGGTG ACCATGCTGA GATAGATGTT 
CCAAAGAGCC AGCAAGCCAC AACCAAAACC ACACTCACAG GTCTAAGGCC AGGAACTGAA 
TATGGAGTGG GCGTCTCTGC TGTTAAGGGA GACAAGGAGG GCGATCCAGC AACCATCAAT 
GCAGCCACAG AAATTGATGC ACCCAGGGAC TTACAGGTGT CTGAGACCAC ACAAGACAGT 
CTGACTTTAT TCTGGAAGAC ACCTCTGGCC AAGTTTGATC GTTACCGCCT CAACTACAGC 
CTCCCCACAG GCCAGTCAAT AGAGATTCAG CTGCCAAAGG ATGCCACCTC CCATGTCCTG 
ACAGACCTGG AGCCAGGGCA AGAATACACT GTTCTCCTCA CCGCTGAGAA GGGCAGACAT 
AAGAGCAAGC CTGCGCGTGT GAAGGCATCC ACAGAAGCTG AACCGGAAGT TGACAACCTT 
CTAGTTTCAG ATGCTACTCC AGACGGTTTC CGTCTGTCCT GGACTGCTGA TGAAGGGATA 
TTCGACAGTT TTGTTATCAG GATCAGAGAT ACCAAAAAGC AATCTGAACC ACAAGAAATA 
ACCCTCCCTT CCCCCGACCG TACCAGGGAC ATAACAGGTC TCAGAGAGGC CACTGAGTAC 
GAAATTGAAC TCTATGGAAT AAGCCGTGGA AGGCGATCCC AGCCAGTCAG TGCCATAGCA 
ACAACAGCCA TGGGTTCTCC GAAGGAAATC ATGTTCTCAG ACATCACTGA AAATGCAGCC 
ACAGTCAGCT GGAGAGCACC GACTGCTCAA GTGGAGAGTT TCCGGATTAC TTATGTGCCT 
GTAACAGGAG GTCCCCCATC CATGGTGACC GTGGATGGGA CGGATACTGA GACCCGACTG 
GTGAGGCTCA CCCCTGGAGT GGAGTACCAC GTCAGTGTGA TTGCCATGAA GGGATTTGAA 
GAAAGTGATC CGGTCTCGGG GTCTCTAATC ACAGCTCTCG ATGGTCCATC TGGCCTTCTG 
ACAGCCAACA TCACAGACTC AGAAGCCTTG GCCATGTGGC AGCCAGCCAT TGCCACTGTA 
GACAGTTATG TCATCTCCTA CACAGGGGAG AGAGTGCCAG AAATTACCCG CACAGTGTCT 
GGGAATACAG TGGAGTTTGA ACTACATGAC CTGGAGCCTG CCACAGAGTA CACACTGAGT 
GTCTTTGCAG AGAAAGGTCA CCAGAAGAGT TCTACCATCG CTACGAAATT TACCACAGAC 
CTGGATTCCC CAAGAGAATT GACTGCTACT GAGGTTCAGT CAGAAACTGC CCTCCTCACC 
TGGAGACCTC CCCGGGCATC GGTCACTGGA TACCTCCTGG TCTATGAGTC TGTGGATGGT 
ACAGTCAAGG AAGTCATTGT GGGGCCTGAC ACCACCTCCT ACAGCCTGGC AGACCTGAGC 
CCATCCACCC ACTACACAGC AAGAATCCAG GCATTGAGTG GGTCCCTGAG GAGCAAACTG 
ATCCAAACCA TCTTCACCAC AATTGGACTC CTATATCCAT TCCCTAGGGA TTGCTCTCAA 
GCAATGTTGA ATGGCGACAC CACCTCTGGC CTCTATACCA TCTATATAAA TGGTGACAAG 
ACTCAAGCAC TGGAAGTCTA CTGTGATATG ACCTCTGATG GAGGTGGATG GATCGTTTTC 
CTGAGACGCA AAAATGGACG TGAGGACTTC TATCGAAACT GGAAGGCCTA TGCCGCTGGG 
TTTGGAGACC GCAGAGAAGA ATTCTGGCTT GGACTGGATA ACCTGAGCAA AATCACAGCC 
CAAGGGCAAT ATGAGCTCCG GGTGGACCTA CAAGACCATG GGGAGTCAGC CTATGCTGTG 
TATGACAGGT TCAGTGTTGG AGATGCCAAG AGTCGCTACA AGCTGAAGGT AGAAGGATAC 
AGTGGAACGG CAGGTGACTC CATGAACTAT CACAATGGTA GATCCTTCTC TACCTATGAC 
AAGGACACAG ACTCAGCCAT CACCAACTGT GCCCTGTCCT ACAAAGGAGC TTTCTGGTAT 
AAGAACTGTC ATCGTGTCAA CCTGATGGGC AGATATGGGG ACAATAATCA CAGTCAGGGT 
GTTAACTGGT TCCACTGGAA GGGCCATGAG TACTCAATCC AGTTTGCGGA GATGAAACTA 
AGACCCAGCA ACTTCCGAAA TCTGGAAGGC AGGCGTAAGC GGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CD73-derived adenosine and tenascin-C control cytokine production by epicardium-derived cells formed after myocardial infarction.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(7)3040-3053(2017 Jul)
Hesse J,Leberling S,Boden E,Friebe D,Schmidt T,Ding Z,Dieterich P,Deussen A,Roderigo C,Rose CR,Floss DM,Scheller J,Schrader J

Tenascin-C expression and its associated pathway in BMSCs following co-culture with mechanically stretched ligament fibroblasts.
Molecular medicine reports15(5)2465-2472(2017 May)
Zhao B,Hu M,Wu H,Ren C,Wang J,Cui S

Early remodelling of the extracellular matrix proteins tenascin-C and phosphacan in retina and optic nerve of an experimental autoimmune glaucoma model.
Journal of cellular and molecular medicine20(11)2122-2137(2016 11)
Reinehr S,Reinhard J,Wiemann S,Stute G,Kuehn S,Woestmann J,Dick HB,Faissner A,Joachim SC

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

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Tnc cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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