Ap2m1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ap2m1
Entrez Gene ID	116563
Full Name	adaptor-related protein complex 2, mu 1 subunit
Synonyms	Ap50,Clapm1,mu2
General protein information	Preferred Names adaptor-related protein complex 2, mu 1 subunit Names AP-2 complex subunit mu AP-2 mu chain adapter-related protein complex 2 mu subunit adapter-related protein complex 2 subunit mu adaptor protein complex AP-2 subunit mu adaptor-related protein complex 2 subunit mu clathrin assembly protein complex 2 medium chain clathrin assembly protein complex 2 mu medium chain clathrin coat assembly protein AP50 clathrin coat-associated protein AP50 coat assembly protein complex 50 kD mu2-adaptin plasma membrane adaptor AP-2 50 kDa protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q23
Summary	encodes the mu subunit of the heterotetrameric coat assembly protein complex 2 (AP2); associated with clathrin-coated vesicles and may be important for intracellular trafficking [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053837.1	NP_446289.1	AP-2 complex subunit mu

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_05484	XP_003710350.1
Gallus gallus (chicken)	AP2M1	NP_001072962.1
Schizosaccharomyces pombe (fission yeast)	apm4	NP_592921.1
Neurospora crassa	NCU09673	XP_960763.1
Pan troglodytes (chimpanzee)	AP2M1	XP_001144097.2
Canis lupus familiaris (dog)	AP2M1	XP_849091.1
Bos taurus (cattle)	AP2M1	NP_001029695.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007131	XP_308629.2
Oryza sativa (rice)	Os02g0690700	NP_001047793.1
Xenopus tropicalis (tropical clawed frog)	ap2m1	NP_988975.1
Mus musculus (house mouse)	Ap2m1	NP_033809.1
Danio rerio (zebrafish)	ap2m1a	NP_997742.1
Danio rerio (zebrafish)	ap2m1b	NP_957320.1
Caenorhabditis elegans (roundworm)	dpy-23	NP_741770.1
Homo sapiens (human)	AP2M1	NP_004059.2
	AP2M1	XP_002802940.1
Rattus norvegicus (Norway rat)	Ap2m1	NP_446289.1
Drosophila melanogaster (fruit fly)	AP-50	NP_001163686.1
Arabidopsis thaliana (thale cress)	AT5G46630	NP_199475.1

Gene Ontology
Bibliography

Related articles in PubMed

Adaptor Protein 2 (AP-2) complex is essential for functional axogenesis in hippocampal neurons.
Kyung JW, Cho IH, Lee S, Song WK, Ryan TA, Hoppa MB, Kim SH
Scientific reports741620(2017 Jan)

Selective integrin endocytosis is driven by interactions between the integrin ?-chain and AP2.
De Franceschi N, Arjonen A, Elkhatib N, Denessiouk K, Wrobel AG, Wilson TA, Pouwels J, Montagnac G, Owen DJ, Ivaska J
Nature structural & molecular biology23(2)172-9(2016 Feb)

CALM regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature.
Miller SE, Mathiasen S, Bright NA, Pierre F, Kelly BT, Kladt N, Schauss A, Merrifield CJ, Stamou D, H?ning S, Owen DJ
Developmental cell33(2)163-75(2015 Apr)

Clathrin adaptors. AP2 controls clathrin polymerization with a membrane-activated switch.
Kelly BT, Graham SC, Liska N, Dannhauser PN, H?ning S, Ungewickell EJ, Owen DJ
Science (New York, N.Y.)345(6195)459-63(2014 Jul)

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kr?hnert K, Sch?fer C, Rammner B, Koo SJ, Cla?en GA, Krauss M, Haucke V, Urlaub H, Rizzoli SO
Science (New York, N.Y.)344(6187)1023-8(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

-2 plays a role in cargo selection but no single aspect of mu2 function is critical, implying that a more distributed network of interactions supports AP-2 function in SV endocytosis
Title: A distributed set of interactions controls mu2 functionality in the role of AP-2 as a sorting adaptor in synaptic vesicle endocytosis.

identify a gamma2-subunit-specific Yxxvarphi-type-binding motif for the clathrin adaptor protein, AP2, which is located within a site for gamma2-subunit tyrosine phosphorylation.
Title: Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit.

The following Ap2m1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ap2m1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10816	NM_053837.1 Latest version!	Rattus norvegicus adaptor related protein complex 2 subunit mu 1 (Ap2m1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ap2m1 ( NM_053837.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446289.1 Rattus norvegicus adaptor related protein complex 2 subunit mu 1 (Ap2m1), mRNA.

Price & Availability↑

CloneID	ORa10816
Clone ID Related Accession (Same CDS sequence)	NM_053837.1
Accession Version	NM_053837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	AP-2 complex subunit mu
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M23674.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M23674.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347 [ECO:0000348] ##Evidence-Data-END##

ATGATCGGAG GCTTATTCAT CTATAATCAC AAGGGGGAGG TGCTTATCTC CCGAGTCTAC 
AGAGATGACA TCGGGAGGAA TGCAGTGGAT GCCTTTCGCG TCAATGTCAT TCACGCACGG 
CAGCAGGTGC GCAGCCCTGT CACAAACATC GCTCGCACCA GCTTCTTCCA TGTTAAGCGG 
TCCAACATCT GGCTGGCAGC AGTCACCAAG CAGAATGTCA ATGCCGCCAT GGTCTTCGAA 
TTCCTCTACA AGATGTGTGA TGTAATGGCT GCTTACTTTG GCAAGATCAG CGAGGAGAAC 
ATCAAGAACA ATTTCGTGCT CATATACGAG CTGCTGGACG AGATTCTGGA CTTTGGCTAC 
CCACAGAACT CAGAGACAGG TGCACTGAAA ACCTTCATCA CCCAACAGGG TATCAAGAGT 
CAGCATCAGA CAAAAGAAGA GCAGTCCCAG ATCACCAGCC AGGTGACCGG GCAAATTGGC 
TGGAGGCGGG AAGGCATCAA GTATCGTCGG AATGAACTCT TCCTAGATGT TCTGGAGAGT 
GTGAACCTGC TTATGTCCCC ACAGGGGCAG GTGCTGAGTG CCCATGTGTC GGGCCGGGTG 
GTGATGAAGA GTTACCTGAG CGGCATGCCT GAGTGCAAGT TTGGAATGAA TGACAAGATT 
GTCATAGAAA AGCAGGGCAA AGGCACAGCC GATGAAACAA GCAAGAGTGG TAAGCAGTCG 
ATCGCCATTG ATGACTGCAC CTTTCACCAG TGTGTGCGAC TCAGCAAGTT TGACTCTGAG 
CGGAGCATCA GCTTTATCCC TCCTGATGGA GAGTTTGAAC TCATGAGATA CCGTACTACC 
AAGGACATCA TCCTGCCTTT CCGGGTGATC CCATTGGTGC GGGAGGTGGG ACGCACCAAA 
CTGGAGGTCA AGGTGGTCAT CAAGTCCAAC TTCAAGCCCT CACTTCTGGC CCAGAAGATT 
GAGGTGAGGA TCCCAACCCC ATTGAACACA AGCGGGGTGC AAGTGATCTG CATGAAGGGG 
AAGGCCAAGT ACAAGGCCAG CGAGAACGCC ATTGTATGGA AGATCAAGCG CATGGCAGGC 
ATGAAGGAAT CACAGATCAG CGCAGAGATT GAGCTCCTGC CCACCAATGA TAAGAAGAAA 
TGGGCTCGGC CCCCCATTTC CATGAACTTT GAGGTGCCAT TTGCGCCTTC TGGCCTCAAG 
GTGCGCTACT TGAAGGTATT TGAGCCGAAG CTGAACTACA GCGACCACGA TGTCATCAAA 
TGGGTGCGCT ACATTGGCCG AAGTGGCATT TACGAAACCC GCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446289.1
CDS	63..1370
Translation	MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC

Target ORF information:

RefSeq Version	NM_053837.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adaptor related protein complex 2 subunit mu 1 (Ap2m1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053837.1

ORF Insert Sequence:

ATGATCGGAG GCTTATTCAT CTATAATCAC AAGGGGGAGG TGCTTATCTC CCGAGTCTAC 
AGAGATGACA TCGGGAGGAA TGCAGTGGAT GCCTTTCGCG TCAATGTCAT TCACGCACGG 
CAGCAGGTGC GCAGCCCTGT CACAAACATC GCTCGCACCA GCTTCTTCCA TGTTAAGCGG 
TCCAACATCT GGCTGGCAGC AGTCACCAAG CAGAATGTCA ATGCCGCCAT GGTCTTCGAA 
TTCCTCTACA AGATGTGTGA TGTAATGGCT GCTTACTTTG GCAAGATCAG CGAGGAGAAC 
ATCAAGAACA ATTTCGTGCT CATATACGAG CTGCTGGACG AGATTCTGGA CTTTGGCTAC 
CCACAGAACT CAGAGACAGG TGCACTGAAA ACCTTCATCA CCCAACAGGG TATCAAGAGT 
CAGCATCAGA CAAAAGAAGA GCAGTCCCAG ATCACCAGCC AGGTGACCGG GCAAATTGGC 
TGGAGGCGGG AAGGCATCAA GTATCGTCGG AATGAACTCT TCCTAGATGT TCTGGAGAGT 
GTGAACCTGC TTATGTCCCC ACAGGGGCAG GTGCTGAGTG CCCATGTGTC GGGCCGGGTG 
GTGATGAAGA GTTACCTGAG CGGCATGCCT GAGTGCAAGT TTGGAATGAA TGACAAGATT 
GTCATAGAAA AGCAGGGCAA AGGCACAGCC GATGAAACAA GCAAGAGTGG TAAGCAGTCG 
ATCGCCATTG ATGACTGCAC CTTTCACCAG TGTGTGCGAC TCAGCAAGTT TGACTCTGAG 
CGGAGCATCA GCTTTATCCC TCCTGATGGA GAGTTTGAAC TCATGAGATA CCGTACTACC 
AAGGACATCA TCCTGCCTTT CCGGGTGATC CCATTGGTGC GGGAGGTGGG ACGCACCAAA 
CTGGAGGTCA AGGTGGTCAT CAAGTCCAAC TTCAAGCCCT CACTTCTGGC CCAGAAGATT 
GAGGTGAGGA TCCCAACCCC ATTGAACACA AGCGGGGTGC AAGTGATCTG CATGAAGGGG 
AAGGCCAAGT ACAAGGCCAG CGAGAACGCC ATTGTATGGA AGATCAAGCG CATGGCAGGC 
ATGAAGGAAT CACAGATCAG CGCAGAGATT GAGCTCCTGC CCACCAATGA TAAGAAGAAA 
TGGGCTCGGC CCCCCATTTC CATGAACTTT GAGGTGCCAT TTGCGCCTTC TGGCCTCAAG 
GTGCGCTACT TGAAGGTATT TGAGCCGAAG CTGAACTACA GCGACCACGA TGTCATCAAA 
TGGGTGCGCT ACATTGGCCG AAGTGGCATT TACGAAACCC GCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Adaptor Protein 2 (AP-2) complex is essential for functional axogenesis in hippocampal neurons.
Scientific reports741620(2017 Jan)
Kyung JW,Cho IH,Lee S,Song WK,Ryan TA,Hoppa MB,Kim SH

Selective integrin endocytosis is driven by interactions between the integrin ?-chain and AP2.
Nature structural & molecular biology23(2)172-9(2016 Feb)
De Franceschi N,Arjonen A,Elkhatib N,Denessiouk K,Wrobel AG,Wilson TA,Pouwels J,Montagnac G,Owen DJ,Ivaska J

CALM regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature.
Developmental cell33(2)163-75(2015 Apr)
Miller SE,Mathiasen S,Bright NA,Pierre F,Kelly BT,Kladt N,Schauss A,Merrifield CJ,Stamou D,H?ning S,Owen DJ

Clathrin adaptors. AP2 controls clathrin polymerization with a membrane-activated switch.
Science (New York, N.Y.)345(6195)459-63(2014 Jul)
Kelly BT,Graham SC,Liska N,Dannhauser PN,H?ning S,Ungewickell EJ,Owen DJ

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Science (New York, N.Y.)344(6187)1023-8(2014 May)
Wilhelm BG,Mandad S,Truckenbrodt S,Kr?hnert K,Sch?fer C,Rammner B,Koo SJ,Cla?en GA,Krauss M,Haucke V,Urlaub H,Rizzoli SO

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Ap2m1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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