Mapk8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mapk8
Entrez Gene ID	116554
Full Name	mitogen-activated protein kinase 8
Synonyms	JNK
General protein information	Preferred Names mitogen-activated protein kinase 8 Names mitogen-activated protein kinase 8 MAP kinase 8 MAPK 8 SAPK gamma c-Jun N-terminal kinase 1 c-jun NH2-terminal kinase p54 gamma stress-activated protein kinase JNK1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p16
Summary	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. [provided by RefSeq, Jul 2012].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053829.2	NP_446281.2	mitogen-activated protein kinase 8

Pathways from BioSystems

Homologies

	MAPK8	XP_001108815.1
Canis lupus familiaris (dog)	MAPK8	XP_005637524.1
Rattus norvegicus (Norway rat)	Mapk8	NP_446281.1
Xenopus tropicalis (tropical clawed frog)	mapk8	NP_001123415.1
Homo sapiens (human)	MAPK8	NP_001265476.1
Bos taurus (cattle)	MAPK8	NP_001179903.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009461	XP_310236.3
Pan troglodytes (chimpanzee)	MAPK8	XP_001136927.1
Gallus gallus (chicken)	MAPK8	XP_001233169.1
Danio rerio (zebrafish)	mapk8a	NP_001103859.1
Drosophila melanogaster (fruit fly)	bsk	NP_001162930.1
Caenorhabditis elegans (roundworm)	jnk-1	NP_001021270.1
Mus musculus (house mouse)	Mapk8	NP_057909.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of the c-Jun N-terminal kinase signaling pathway in the activation of trypsinogen in rat pancreatic acinar cells.
Yang Z, Yang W, Lu M, Li Z, Qiao X, Sun B, Zhang W, Xue D
International journal of molecular medicine41(2)1119-1126(2018 Feb)

Capture of Dense Core Vesicles at Synapses by JNK-Dependent Phosphorylation of Synaptotagmin-4.
Bharat V, Siebrecht M, Burk K, Ahmed S, Reissner C, Kohansal-Nodehi M, Steubler V, Zweckstetter M, Ting JT, Dean C
Cell reports21(8)2118-2133(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest anti-inflammatory roles of CTGF-stimulated tendon stem cells that are likely associated with improved tendon healing.
Title: Tendon stem/progenitor cells regulate inflammation in tendon healing

this study suggests a link between melatonin, JNK, FoxO1 and IDO1 that acts as a potential balance regulator of tryptophan metabolism, and offers a new approach to treat diseases related to dysregulation of tryptophan metabolism
Title: Regulating the balance between the kynurenine and serotonin pathways of tryptophan metabolism.

JNK1 activation plays a role in the biological link between depression and NASH. Sitagliptin significantly decreased expression of hepatic JNK1 in rat model of NAFLD with mild chronic stress.
Title: Potential involvement of JNK1 repression in the hepatic effect of sitagliptin and metformin in rats subjected to high fat diet and chronic mild distress.

Ischemia/reperfusion-induced JNK phosphorylation was reduced by SP600125, indicating that JNK mediates the apoptosis pathways in rat skin.
Title: Inhibition of c-Jun N-terminal kinase signaling suppresses skin flap apoptosis in a rat ischemia and/or reperfusion model.

The activation of JNK and p38 MAPK pathways in OGD-treated PC12 cells was suppressed by exogenous Act A and enhanced by ActRIIA-Ab. Together, our results suggest that Act A/Smads signaling pathway negatively regulates OGD-induced autophagy via suppression of JNK and p38 MAPK pathways in neuronal PC12 cells.
Title: CHOP deficiency inhibits methylglyoxal-induced endothelial dysfunction.

The following Mapk8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mapk8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa44003	NM_053829.2 Latest version!	Rattus norvegicus mitogen-activated protein kinase 8 (Mapk8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mapk8 ( NM_053829.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446281.2 Rattus norvegicus mitogen-activated protein kinase 8 (Mapk8), mRNA.

Price & Availability↑

CloneID	ORa44003
Clone ID Related Accession (Same CDS sequence)	NM_053829.2
Accession Version	NM_053829.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Rattus norvegicus(Norway rat)
Product	mitogen-activated protein kinase 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07024627.1, CB782828.1, L27129.1, AABR07024624.1, FM076271.1, DV724511.1 and AA963835.1. On Oct 7, 2014 this sequence version replaced NM_053829.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L27129.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCAGAA GTAAACGTGA CAACAATTTT TATAGTGTAG AGATCGGAGA TTCTACATTC 
ACAGTCCTAA AACGATACCA GAACTTAAAG CCTATAGGCT CAGGAGCTCA AGGAATAGTG 
TGTGCAGCTT ATGATGCTAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT CAGCCGGCCA 
TTTCAGAATC AGACCCATGC TAAGCGAGCC TACCGAGAAC TAGTTCTTAT GAAGTGTGTT 
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA 
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG 
ATGGAGTTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AAATGCTGTG TGGAATCAAG 
CACCTTCACT CTGCTGGAAT TATTCATCGG GACTTAAAGC CTAGTAATAT AGTAGTCAAA 
TCAGACTGCA CTTTGAAGAT TCTTGATTTT GGACTGGCAA GGACTGCAGG AACGAGTTTT 
ATGATGACGC CTTACGTGGT AACTCGTTAC TACAGAGCAC CAGAGGTCAT TCTCGGCATG 
GGCTACAAGG AGAACGTGGA TTTATGGTCT GTGGGGTGCA TTATGGGAGA AATGGTTTGC 
CTCAAAATCC TCTTTCCAGG AAGGGACTAT ATTGATCAGT GGAATAAAGT TATTGAACAG 
CTCGGAACAC CTTGTCCTGA ATTCATGAAG AAACTACAAC CAACAGTAAG GACTTACGTT 
GAAAACAGAC CTAAGTACGC TGGCTATAGC TTTGAGAAAC TGTTTCCTGA TGTGCTTTTC 
CCAGCTGACT CAGAACATAA CAAACTTAAA GCCAGTCAGG CGAGAGATTT GTTATCTAAA 
ATGCTGGTGA TAGATGCGTC CAAAAGGATC TCCGTAGACG AAGCTCTCCA GCACCCGTAC 
ATCAACGTCT GGTATGATCC TTCAGAAGCA GAGGCCCCAC CACCAAAGAT CCCTGACAAG 
CAGTTAGATG AAAGGGAGCA CACAATAGAG GAGTGGAAAG AACTGATATA CAAGGAGGTC 
ATGGATTTGG AGGAGCGAAC TAAGAATGGC GTCATAAGAG GGCAGCCGTC TCCTTTAGGT 
GCAGCAGTGA TCAATGGCTC TCAGCATCCG GTCTCTTCGC CGTCTGTCAA TGACATGTCT 
TCAATGTCCA CAGATCCGAC TCTGGCCTCG GATACAGACA GCAGTCTAGA AGCATCAGCT 
GGACCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446281.2
CDS	176..1459
Translation	MSRSKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCLKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDLEERTKNGVIRGQPSPLGAAVINGSQHPVSSPSVNDMSSMSTDPTLASDTDSSLEASAGPLGCCR

Target ORF information:

RefSeq Version	NM_053829.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen-activated protein kinase 8 (Mapk8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053829.2

ORF Insert Sequence:

ATGAGCAGAA GTAAACGTGA CAACAATTTT TATAGTGTAG AGATCGGAGA TTCTACATTC 
ACAGTCCTAA AACGATACCA GAACTTAAAG CCTATAGGCT CAGGAGCTCA AGGAATAGTG 
TGTGCAGCTT ATGATGCTAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT CAGCCGGCCA 
TTTCAGAATC AGACCCATGC TAAGCGAGCC TACCGAGAAC TAGTTCTTAT GAAGTGTGTT 
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA 
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG 
ATGGAGTTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AAATGCTGTG TGGAATCAAG 
CACCTTCACT CTGCTGGAAT TATTCATCGG GACTTAAAGC CTAGTAATAT AGTAGTCAAA 
TCAGACTGCA CTTTGAAGAT TCTTGATTTT GGACTGGCAA GGACTGCAGG AACGAGTTTT 
ATGATGACGC CTTACGTGGT AACTCGTTAC TACAGAGCAC CAGAGGTCAT TCTCGGCATG 
GGCTACAAGG AGAACGTGGA TTTATGGTCT GTGGGGTGCA TTATGGGAGA AATGGTTTGC 
CTCAAAATCC TCTTTCCAGG AAGGGACTAT ATTGATCAGT GGAATAAAGT TATTGAACAG 
CTCGGAACAC CTTGTCCTGA ATTCATGAAG AAACTACAAC CAACAGTAAG GACTTACGTT 
GAAAACAGAC CTAAGTACGC TGGCTATAGC TTTGAGAAAC TGTTTCCTGA TGTGCTTTTC 
CCAGCTGACT CAGAACATAA CAAACTTAAA GCCAGTCAGG CGAGAGATTT GTTATCTAAA 
ATGCTGGTGA TAGATGCGTC CAAAAGGATC TCCGTAGACG AAGCTCTCCA GCACCCGTAC 
ATCAACGTCT GGTATGATCC TTCAGAAGCA GAGGCCCCAC CACCAAAGAT CCCTGACAAG 
CAGTTAGATG AAAGGGAGCA CACAATAGAG GAGTGGAAAG AACTGATATA CAAGGAGGTC 
ATGGATTTGG AGGAGCGAAC TAAGAATGGC GTCATAAGAG GGCAGCCGTC TCCTTTAGGT 
GCAGCAGTGA TCAATGGCTC TCAGCATCCG GTCTCTTCGC CGTCTGTCAA TGACATGTCT 
TCAATGTCCA CAGATCCGAC TCTGGCCTCG GATACAGACA GCAGTCTAGA AGCATCAGCT 
GGACCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of the c-Jun N-terminal kinase signaling pathway in the activation of trypsinogen in rat pancreatic acinar cells.
International journal of molecular medicine41(2)1119-1126(2018 Feb)
Yang Z,Yang W,Lu M,Li Z,Qiao X,Sun B,Zhang W,Xue D

Capture of Dense Core Vesicles at Synapses by JNK-Dependent Phosphorylation of Synaptotagmin-4.
Cell reports21(8)2118-2133(2017 Nov)
Bharat V,Siebrecht M,Burk K,Ahmed S,Reissner C,Kohansal-Nodehi M,Steubler V,Zweckstetter M,Ting JT,Dean C

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Mapk8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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