Ralb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ralb
Entrez Gene ID	116546
Full Name	RAS like proto-oncogene B
Synonyms	dRalb
General protein information	Preferred Names RAS like proto-oncogene B Names ras-related protein Ral-B GTP binding protein) RALB Ras like proto-oncogene B v-ral simian leukemia viral oncogene homolog B (ras related) v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q11
Summary	putative GTP binding protein [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017598636.1	XP_017454125.1	ras-related protein Ral-B isoform X1
NM_053821.2	NP_446273.1	ras-related protein Ral-B precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RALB	XP_541001.2
Mus musculus (house mouse)	Ralb	NP_071722.1
Danio rerio (zebrafish)	ralba	NP_001003649.1
Homo sapiens (human)	RALB	NP_002872.1
Pan troglodytes (chimpanzee)	RALB	XP_001172858.2
	RALB	XP_001084976.1
Gallus gallus (chicken)	RALB	XP_422085.1
Bos taurus (cattle)	RALB	NP_001091454.1
Rattus norvegicus (Norway rat)	Ralb	NP_446273.1

Gene Ontology
Bibliography

Related articles in PubMed

The deubiquitylase USP33 discriminates between RALB functions in autophagy and innate immune response.
Simicek M, Lievens S, Laga M, Guzenko D, Aushev VN, Kalev P, Baietti MF, Strelkov SV, Gevaert K, Tavernier J, Sablina AA
Nature cell biology15(10)1220-30(2013 Oct)

Phosphorylation by protein kinase C? regulates RalB small GTPase protein activation, subcellular localization, and effector utilization.
Martin TD, Mitin N, Cox AD, Yeh JJ, Der CJ
The Journal of biological chemistry287(18)14827-36(2012 Apr)

Solution structure and dynamics of the small GTPase RalB in its active conformation: significance for effector protein binding.
Fenwick RB, Prasannan S, Campbell LJ, Nietlispach D, Evetts KA, Camonis J, Mott HR, Owen D
Biochemistry48(10)2192-206(2009 Mar)

Distinct roles of RalA and RalB in the progression of cytokinesis are supported by distinct RalGEFs.
Cascone I, Selimoglu R, Ozdemir C, Del Nery E, Yeaman C, White M, Camonis J
The EMBO journal27(18)2375-87(2008 Sep)

Geranylgeranyltransferase I inhibitors target RalB to inhibit anchorage-dependent growth and induce apoptosis and RalA to inhibit anchorage-independent growth.
Falsetti SC, Wang DA, Peng H, Carrico D, Cox AD, Der CJ, Hamilton AD, Sebti SM
Molecular and cellular biology27(22)8003-14(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

phosphorylation by PKCalpha is critical for RalB-mediated vesicle trafficking and exocytosis.
Title: Phosphorylation by protein kinase C? regulates RalB small GTPase protein activation, subcellular localization, and effector utilization.

Our results indicate that endogenous RalA and RalB function specifically as GTP sensors for the GTP-dependent exocytosis of dense core vesicles.
Title: RalA and RalB function as the critical GTP sensors for GTP-dependent exocytosis.

RalB regulation of exocyst function is required for the coordinated delivery of secretory vesicles to the sites of dynamic plasma membrane expansion that specify directional movement.
Title: RalB mobilizes the exocyst to drive cell migration.

The two Ral isoforms, RalA and -B, promote branching through distinct pathways, involving the exocyst complex and phospholipase D, respectively.
Title: Ral GTPases regulate neurite branching through GAP-43 and the exocyst complex.

The following Ralb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ralb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10764	NM_053821.2 Latest version!	Rattus norvegicus RAS like proto-oncogene B (Ralb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa10764	XM_017598636.1 Latest version!	Rattus norvegicus RALB Ras like proto-oncogene B (Ralb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ralb ( NM_053821.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446273.1 Rattus norvegicus RAS like proto-oncogene B (Ralb), mRNA.

Price & Availability↑

CloneID	ORa10764
Clone ID Related Accession (Same CDS sequence)	XM_017598636.1 , NM_053821.2
Accession Version	NM_053821.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	ras-related protein Ral-B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC072505.1. On Jun 13, 2004 this sequence version replaced NM_053821.1. ##Evidence-Data-START## Transcript exon combination :: BC072505.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCCA ACAAAGGCAA GAGCCAGGGC TCCCTGGTCC TTCACAAGGT CATCATGGTC 
GGCAGTGGGG GAGTCGGCAA GTCAGCCCTG ACGCTCCAGT TCATGTATGA CGAGTTTGTA 
GAAGACTATG AGCCCACCAA AGCCGACAGT TACAGAAAGA AGGTGGTTCT CGACGGAGAA 
GAGGTTCAGA TAGACATCCT GGACACAGCC GGCCAGGAGG ACTATGCGGC CATCCGTGAC 
AACTACTTCC GAAGCGGAGA AGGCTTTCTG CTGGTGTTCT CCATCACGGA GCACGAGTCT 
TTCACCGCCA CAGCTGAGTT CAGGGAACAG ATCCTCCGAG TCAAGGCCGA GGAGGACAAA 
ATCCCTCTGC TGGTCGTGGG CAACAAGTCT GACCTGGAGG AGCGGAGGCA GGTGCCCGTG 
GAGGAGGCCC GAGGCAAAGC GGAAGAGTGG GGTGTGCAGT ATGTGGAGAC ATCCGCAAAG 
ACCCGAGCCA ACGTGGACAA GGTGTTCTTT GATCTGATGA GAGAAATCCG GGCAAAAAAG 
ATGTCGGAAA ACAAGGACAA GAACGGCAGG AAAAGCGGCA AGAGCAAGAA GAGTTTCAAA 
GAGCGGTGCT GCTTGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446273.1
CDS	217..837
Translation	MAANKGKSQGSLVLHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLLVFSITEHESFTATAEFREQILRVKAEEDKIPLLVVGNKSDLEERRQVPVEEARGKAEEWGVQYVETSAKTRANVDKVFFDLMREIRAKKMSENKDKNGRKSGKSKKSFKERCCLL

Target ORF information:

RefSeq Version	NM_053821.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAS like proto-oncogene B (Ralb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053821.2

ORF Insert Sequence:

ATGGCTGCCA ACAAAGGCAA GAGCCAGGGC TCCCTGGTCC TTCACAAGGT CATCATGGTC 
GGCAGTGGGG GAGTCGGCAA GTCAGCCCTG ACGCTCCAGT TCATGTATGA CGAGTTTGTA 
GAAGACTATG AGCCCACCAA AGCCGACAGT TACAGAAAGA AGGTGGTTCT CGACGGAGAA 
GAGGTTCAGA TAGACATCCT GGACACAGCC GGCCAGGAGG ACTATGCGGC CATCCGTGAC 
AACTACTTCC GAAGCGGAGA AGGCTTTCTG CTGGTGTTCT CCATCACGGA GCACGAGTCT 
TTCACCGCCA CAGCTGAGTT CAGGGAACAG ATCCTCCGAG TCAAGGCCGA GGAGGACAAA 
ATCCCTCTGC TGGTCGTGGG CAACAAGTCT GACCTGGAGG AGCGGAGGCA GGTGCCCGTG 
GAGGAGGCCC GAGGCAAAGC GGAAGAGTGG GGTGTGCAGT ATGTGGAGAC ATCCGCAAAG 
ACCCGAGCCA ACGTGGACAA GGTGTTCTTT GATCTGATGA GAGAAATCCG GGCAAAAAAG 
ATGTCGGAAA ACAAGGACAA GAACGGCAGG AAAAGCGGCA AGAGCAAGAA GAGTTTCAAA 
GAGCGGTGCT GCTTGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ralb ( XM_017598636.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454125.1 Rattus norvegicus RALB Ras like proto-oncogene B (Ralb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa10764
Clone ID Related Accession (Same CDS sequence)	XM_017598636.1 , NM_053821.2
Accession Version	XM_017598636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ras-related protein Ral-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) and transcript sequence (BC072505.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 103 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCTGCCA ACAAAGGCAA GAGCCAGGGC TCCCTGGTCC TTCACAAGGT CATCATGGTC 
GGCAGTGGGG GAGTCGGCAA GTCAGCCCTG ACGCTCCAGT TCATGTATGA CGAGTTTGTA 
GAAGACTATG AGCCCACCAA AGCCGACAGT TACAGAAAGA AGGTGGTTCT CGACGGAGAA 
GAGGTTCAGA TAGACATCCT GGACACAGCC GGCCAGGAGG ACTATGCGGC CATCCGTGAC 
AACTACTTCC GAAGCGGAGA AGGCTTTCTG CTGGTGTTCT CCATCACGGA GCACGAGTCT 
TTCACCGCCA CAGCTGAGTT CAGGGAACAG ATCCTCCGAG TCAAGGCCGA GGAGGACAAA 
ATCCCTCTGC TGGTCGTGGG CAACAAGTCT GACCTGGAGG AGCGGAGGCA GGTGCCCGTG 
GAGGAGGCCC GAGGCAAAGC GGAAGAGTGG GGTGTGCAGT ATGTGGAGAC ATCCGCAAAG 
ACCCGAGCCA ACGTGGACAA GGTGTTCTTT GATCTGATGA GAGAAATCCG GGCAAAAAAG 
ATGTCGGAAA ACAAGGACAA GAACGGCAGG AAAAGCGGCA AGAGCAAGAA GAGTTTCAAA 
GAGCGGTGCT GCTTGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454125.1
CDS	235..855
Translation	MAANKGKSQGSLVLHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLLVFSITEHESFTATAEFREQILRVKAEEDKIPLLVVGNKSDLEERRQVPVEEARGKAEEWGVQYVETSAKTRANVDKVFFDLMREIRAKKMSENKDKNGRKSGKSKKSFKERCCLL

Target ORF information:

RefSeq Version	XM_017598636.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RALB Ras like proto-oncogene B (Ralb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598636.1

ORF Insert Sequence:

ATGGCTGCCA ACAAAGGCAA GAGCCAGGGC TCCCTGGTCC TTCACAAGGT CATCATGGTC 
GGCAGTGGGG GAGTCGGCAA GTCAGCCCTG ACGCTCCAGT TCATGTATGA CGAGTTTGTA 
GAAGACTATG AGCCCACCAA AGCCGACAGT TACAGAAAGA AGGTGGTTCT CGACGGAGAA 
GAGGTTCAGA TAGACATCCT GGACACAGCC GGCCAGGAGG ACTATGCGGC CATCCGTGAC 
AACTACTTCC GAAGCGGAGA AGGCTTTCTG CTGGTGTTCT CCATCACGGA GCACGAGTCT 
TTCACCGCCA CAGCTGAGTT CAGGGAACAG ATCCTCCGAG TCAAGGCCGA GGAGGACAAA 
ATCCCTCTGC TGGTCGTGGG CAACAAGTCT GACCTGGAGG AGCGGAGGCA GGTGCCCGTG 
GAGGAGGCCC GAGGCAAAGC GGAAGAGTGG GGTGTGCAGT ATGTGGAGAC ATCCGCAAAG 
ACCCGAGCCA ACGTGGACAA GGTGTTCTTT GATCTGATGA GAGAAATCCG GGCAAAAAAG 
ATGTCGGAAA ACAAGGACAA GAACGGCAGG AAAAGCGGCA AGAGCAAGAA GAGTTTCAAA 
GAGCGGTGCT GCTTGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The deubiquitylase USP33 discriminates between RALB functions in autophagy and innate immune response.
Nature cell biology15(10)1220-30(2013 Oct)
Simicek M,Lievens S,Laga M,Guzenko D,Aushev VN,Kalev P,Baietti MF,Strelkov SV,Gevaert K,Tavernier J,Sablina AA

Phosphorylation by protein kinase C? regulates RalB small GTPase protein activation, subcellular localization, and effector utilization.
The Journal of biological chemistry287(18)14827-36(2012 Apr)
Martin TD,Mitin N,Cox AD,Yeh JJ,Der CJ

Solution structure and dynamics of the small GTPase RalB in its active conformation: significance for effector protein binding.
Biochemistry48(10)2192-206(2009 Mar)
Fenwick RB,Prasannan S,Campbell LJ,Nietlispach D,Evetts KA,Camonis J,Mott HR,Owen D

Distinct roles of RalA and RalB in the progression of cytokinesis are supported by distinct RalGEFs.
The EMBO journal27(18)2375-87(2008 Sep)
Cascone I,Selimoglu R,Ozdemir C,Del Nery E,Yeaman C,White M,Camonis J

Geranylgeranyltransferase I inhibitors target RalB to inhibit anchorage-dependent growth and induce apoptosis and RalA to inhibit anchorage-independent growth.
Molecular and cellular biology27(22)8003-14(2007 Nov)
Falsetti SC,Wang DA,Peng H,Carrico D,Cox AD,Der CJ,Hamilton AD,Sebti SM

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Ralb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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