Bak1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bak1
Entrez Gene ID	116502
Full Name	BCL2-antagonist/killer 1
Synonyms	Bak
General protein information	Preferred Names BCL2-antagonist/killer 1 Names bcl-2 homologous antagonist/killer
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12

mRNA and Protein(s)

mRNA	Protein	Name
XM_006256101.3	XP_006256163.1	bcl-2 homologous antagonist/killer isoform X1
NM_053812.1	NP_446264.1	bcl-2 homologous antagonist/killer

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BAK1	XP_003950851.1
Bos taurus (cattle)	BAK1	NP_001071386.1
Canis lupus familiaris (dog)	LOC481744	NP_001018644.1
Rattus norvegicus (Norway rat)	Bak1	NP_446264.1
	BAK1	XP_002804035.1
Mus musculus (house mouse)	Bak1	NP_031549.2
Gallus gallus (chicken)	BAK1	NP_001026091.1
Homo sapiens (human)	BAK1	NP_001179.1

Gene Ontology
Bibliography

Related articles in PubMed

A carvedilol-responsive microRNA, miR-125b-5p protects the heart from acute myocardial infarction by repressing pro-apoptotic bak1 and klf13 in cardiomyocytes.
Bayoumi AS, Park KM, Wang Y, Teoh JP, Aonuma T, Tang Y, Su H, Weintraub NL, Kim IM
Journal of molecular and cellular cardiology11472-82(2017 Nov)

BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.
Llambi F, Wang YM, Victor B, Yang M, Schneider DM, Gingras S, Parsons MJ, Zheng JH, Brown SA, Pelletier S, Moldoveanu T, Chen T, Green DR
Cell165(2)421-33(2016 Apr)

Plasminogen kringle 5 induces endothelial cell apoptosis by triggering a voltage-dependent anion channel 1 (VDAC1) positive feedback loop.
Li L, Yao YC, Gu XQ, Che D, Ma CQ, Dai ZY, Li C, Zhou T, Cai WB, Yang ZH, Yang X, Gao GQ
The Journal of biological chemistry289(47)32628-38(2014 Nov)

BAK multimerization for apoptosis, but not bid binding, is inhibited by negatively charged residue in the BAK hydrophobic groove.
Azad A, Storey A
Molecular cancer1265(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

qPCR analysis performed at the early phase after ischemia revealed a time dependent decrease in Bax, Bak, and Bcl-x(L) and no alteration in Bcl-2 mRNA expression in response to retinal ischemia.
Title: Retinal ischemia-induced apoptosis is associated with alteration in Bax and Bcl-x(L) expression rather than modifications in Bak and Bcl-2.

mitochondrial ROS formation, particularly at respiratory chain complex III, is involved in BAK-induced monolayer hyperpermeability
Title: Mitochondrial complex III is involved in proapoptotic BAK-induced microvascular endothelial cell hyperpermeability.

Bcl-2, Bid, and Bad inhibit assembly sub-stoichiometrically, whereas peptides from Bak and Bax promote tubulin polymerization at near stoichiometric concentrations
Title: Direct interaction of Bcl-2 proteins with tubulin.

the Hexokinase-mitochondria interaction mediated by Akt has a role in inhibiting apoptosis but does not require Bax and Bak
Title: Hexokinase-mitochondria interaction mediated by Akt is required to inhibit apoptosis in the presence or absence of Bax and Bak.

The following Bak1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bak1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10725	NM_053812.1 Latest version!	Rattus norvegicus BCL2-antagonist/killer 1 (Bak1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa10725	XM_006256101.3 Latest version!	Rattus norvegicus BCL2-antagonist/killer 1 (Bak1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bak1 ( NM_053812.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446264.1 Rattus norvegicus BCL2-antagonist/killer 1 (Bak1), mRNA.

Price & Availability↑

CloneID	ORa10725
Clone ID Related Accession (Same CDS sequence)	XM_006256101.3 , NM_053812.1
Accession Version	NM_053812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Rattus norvegicus(Norway rat)
Product	bcl-2 homologous antagonist/killer
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF259504.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF259504.1, BC089784.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCCG GACAAGGACC AGGTCCTCCC AAGGGGGACT GCGATGAGGC CCTGTCTGCT 
TCTGAACAGC AAGTTGCCCA GGACACAGAG GAGGTCTTTC GAAGCTACGT TTTTTACCTC 
CACCAGCAGG AACAAGAGAC CCAGGGGGCA GCCGCCCCTG CTAACCCTGA GATGGACAAC 
TTGTCCCTAG AACCCAACAG TGTCTTGGGT CAGGTGGGTC GGCAGCTTGC TCTCATCGGA 
GACGATATTA ATCGGCGCTA CGACACGGAG TTCCAGAATT TACTGGAACA GCTTCAGCCC 
ACCGCTGGGA ATGCCTACGA ACTCTTCACC AAGATCGCCT CCAGCCTATT TAAGAGCGGC 
ATCAGCTGGG GCCGTGTGGT GGCTCTCCTG GGCTTTGGCT ACCGTCTGGC CCTGTATGTC 
TACCAGCGTG GTTTGACTGG CTTCCTGGGC CAGGTGACCT GCTTTTTGGC TGATATCATA 
CTACACCATT ACATTGCCAG ATGGATTGCA CAGAGAGGTG GTTGGGTGGC AGCCCTGAGT 
TTGCGTAGAG ACCCCATCCT GAGTGTAGTG GTAATTTTTG GTGTGGTTCT GTTGGGCCAA 
TTTGTGGTAC ACAGATTCTT CAGATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446264.1
CDS	246..875
Translation	MASGQGPGPPKGDCDEALSASEQQVAQDTEEVFRSYVFYLHQQEQETQGAAAPANPEMDNLSLEPNSVLGQVGRQLALIGDDINRRYDTEFQNLLEQLQPTAGNAYELFTKIASSLFKSGISWGRVVALLGFGYRLALYVYQRGLTGFLGQVTCFLADIILHHYIARWIAQRGGWVAALSLRRDPILSVVVIFGVVLLGQFVVHRFFRS

Target ORF information:

RefSeq Version	NM_053812.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BCL2-antagonist/killer 1 (Bak1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053812.1

ORF Insert Sequence:

ATGGCATCCG GACAAGGACC AGGTCCTCCC AAGGGGGACT GCGATGAGGC CCTGTCTGCT 
TCTGAACAGC AAGTTGCCCA GGACACAGAG GAGGTCTTTC GAAGCTACGT TTTTTACCTC 
CACCAGCAGG AACAAGAGAC CCAGGGGGCA GCCGCCCCTG CTAACCCTGA GATGGACAAC 
TTGTCCCTAG AACCCAACAG TGTCTTGGGT CAGGTGGGTC GGCAGCTTGC TCTCATCGGA 
GACGATATTA ATCGGCGCTA CGACACGGAG TTCCAGAATT TACTGGAACA GCTTCAGCCC 
ACCGCTGGGA ATGCCTACGA ACTCTTCACC AAGATCGCCT CCAGCCTATT TAAGAGCGGC 
ATCAGCTGGG GCCGTGTGGT GGCTCTCCTG GGCTTTGGCT ACCGTCTGGC CCTGTATGTC 
TACCAGCGTG GTTTGACTGG CTTCCTGGGC CAGGTGACCT GCTTTTTGGC TGATATCATA 
CTACACCATT ACATTGCCAG ATGGATTGCA CAGAGAGGTG GTTGGGTGGC AGCCCTGAGT 
TTGCGTAGAG ACCCCATCCT GAGTGTAGTG GTAATTTTTG GTGTGGTTCT GTTGGGCCAA 
TTTGTGGTAC ACAGATTCTT CAGATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bak1 ( XM_006256101.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256163.1 Rattus norvegicus BCL2-antagonist/killer 1 (Bak1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa10725
Clone ID Related Accession (Same CDS sequence)	XM_006256101.3 , NM_053812.1
Accession Version	XM_006256101.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bcl-2 homologous antagonist/killer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256101.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCCG GACAAGGACC AGGTCCTCCC AAGGGGGACT GCGATGAGGC CCTGTCTGCT 
TCTGAACAGC AAGTTGCCCA GGACACAGAG GAGGTCTTTC GAAGCTACGT TTTTTACCTC 
CACCAGCAGG AACAAGAGAC CCAGGGGGCA GCCGCCCCTG CTAACCCTGA GATGGACAAC 
TTGTCCCTAG AACCCAACAG TGTCTTGGGT CAGGTGGGTC GGCAGCTTGC TCTCATCGGA 
GACGATATTA ATCGGCGCTA CGACACGGAG TTCCAGAATT TACTGGAACA GCTTCAGCCC 
ACCGCTGGGA ATGCCTACGA ACTCTTCACC AAGATCGCCT CCAGCCTATT TAAGAGCGGC 
ATCAGCTGGG GCCGTGTGGT GGCTCTCCTG GGCTTTGGCT ACCGTCTGGC CCTGTATGTC 
TACCAGCGTG GTTTGACTGG CTTCCTGGGC CAGGTGACCT GCTTTTTGGC TGATATCATA 
CTACACCATT ACATTGCCAG ATGGATTGCA CAGAGAGGTG GTTGGGTGGC AGCCCTGAGT 
TTGCGTAGAG ACCCCATCCT GAGTGTAGTG GTAATTTTTG GTGTGGTTCT GTTGGGCCAA 
TTTGTGGTAC ACAGATTCTT CAGATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256163.1
CDS	881..1510
Translation	MASGQGPGPPKGDCDEALSASEQQVAQDTEEVFRSYVFYLHQQEQETQGAAAPANPEMDNLSLEPNSVLGQVGRQLALIGDDINRRYDTEFQNLLEQLQPTAGNAYELFTKIASSLFKSGISWGRVVALLGFGYRLALYVYQRGLTGFLGQVTCFLADIILHHYIARWIAQRGGWVAALSLRRDPILSVVVIFGVVLLGQFVVHRFFRS

Target ORF information:

RefSeq Version	XM_006256101.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BCL2-antagonist/killer 1 (Bak1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256101.3

ORF Insert Sequence:

ATGGCATCCG GACAAGGACC AGGTCCTCCC AAGGGGGACT GCGATGAGGC CCTGTCTGCT 
TCTGAACAGC AAGTTGCCCA GGACACAGAG GAGGTCTTTC GAAGCTACGT TTTTTACCTC 
CACCAGCAGG AACAAGAGAC CCAGGGGGCA GCCGCCCCTG CTAACCCTGA GATGGACAAC 
TTGTCCCTAG AACCCAACAG TGTCTTGGGT CAGGTGGGTC GGCAGCTTGC TCTCATCGGA 
GACGATATTA ATCGGCGCTA CGACACGGAG TTCCAGAATT TACTGGAACA GCTTCAGCCC 
ACCGCTGGGA ATGCCTACGA ACTCTTCACC AAGATCGCCT CCAGCCTATT TAAGAGCGGC 
ATCAGCTGGG GCCGTGTGGT GGCTCTCCTG GGCTTTGGCT ACCGTCTGGC CCTGTATGTC 
TACCAGCGTG GTTTGACTGG CTTCCTGGGC CAGGTGACCT GCTTTTTGGC TGATATCATA 
CTACACCATT ACATTGCCAG ATGGATTGCA CAGAGAGGTG GTTGGGTGGC AGCCCTGAGT 
TTGCGTAGAG ACCCCATCCT GAGTGTAGTG GTAATTTTTG GTGTGGTTCT GTTGGGCCAA 
TTTGTGGTAC ACAGATTCTT CAGATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A carvedilol-responsive microRNA, miR-125b-5p protects the heart from acute myocardial infarction by repressing pro-apoptotic bak1 and klf13 in cardiomyocytes.
Journal of molecular and cellular cardiology11472-82(2017 Nov)
Bayoumi AS,Park KM,Wang Y,Teoh JP,Aonuma T,Tang Y,Su H,Weintraub NL,Kim IM

BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.
Cell165(2)421-33(2016 Apr)
Llambi F,Wang YM,Victor B,Yang M,Schneider DM,Gingras S,Parsons MJ,Zheng JH,Brown SA,Pelletier S,Moldoveanu T,Chen T,Green DR

Plasminogen kringle 5 induces endothelial cell apoptosis by triggering a voltage-dependent anion channel 1 (VDAC1) positive feedback loop.
The Journal of biological chemistry289(47)32628-38(2014 Nov)
Li L,Yao YC,Gu XQ,Che D,Ma CQ,Dai ZY,Li C,Zhou T,Cai WB,Yang ZH,Yang X,Gao GQ

BAK multimerization for apoptosis, but not bid binding, is inhibited by negatively charged residue in the BAK hydrophobic groove.
Molecular cancer1265(2013 Jun)
Azad A,Storey A

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Bak1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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