Dusp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dusp1
Entrez Gene ID	114856
Full Name	dual specificity phosphatase 1
Synonyms	3CH134,CL100,MKP-1,Mkp1,Ptpn16
General protein information	Preferred Names dual specificity phosphatase 1 Names dual specificity protein phosphatase 1 3CH134/CL100 PTPase MAP kinase phosphatase-1 mitogen-activated protein (MAP) kinase phosphatase-1 mitogen-activated protein kinase phosphatase 1 oxidative stress-inducible protein tyrosine phosphatase protein-tyrosine phosphatase CL100 protein-tyrosine phosphatase non-receptor type 16
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12
Summary	This gene encodes a protein that catalyzes the dephosphorylation and inactivation of MAP kinase, and may be involved in insulin mediated signalling. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053769.3	NP_446221.2	dual specificity protein phosphatase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DUSP1	NP_004408.1
Pan troglodytes (chimpanzee)	DUSP1	XP_527120.2
	DUSP1	XP_001096004.1
Gallus gallus (chicken)	DUSP1	NP_001078828.1
Danio rerio (zebrafish)	dusp1	NP_998232.1
Bos taurus (cattle)	DUSP1	NP_001039917.1
Mus musculus (house mouse)	Dusp1	NP_038670.1
Xenopus tropicalis (tropical clawed frog)	dusp1	NP_001005450.2
Canis lupus familiaris (dog)	DUSP1	XP_005619503.1
Rattus norvegicus (Norway rat)	Dusp1	NP_446221.2

Gene Ontology
Bibliography

Related articles in PubMed

Reactive oxygen species overproduction and MAP kinase phosphatase-1 degradation are associated with gastroparesis in a streptozotocin-induced male diabetic rat model.
Smiley R, Naik P, McCallum R, Showkat Ali M
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society(2017 Nov)

MicroRNA-101 in the ventrolateral orbital cortex (VLO) modulates depressive-like behaviors in rats and targets dual-specificity phosphatase 1 (DUSP1).
Zhao Y, Wang S, Chu Z, Dang Y, Zhu J, Su X
Brain research166955-62(2017 Aug)

The Role of MKP-1 in Insulin-Induced Cardioprotection.
Webster I, Smith A, Lochner A, Huisamen B
Cardiovascular drugs and therapy31(3)247-254(2017 Jun)

DUSP-1 gene expression is not regulated by promoter methylation in diabetes-associated cardiac hypertrophy.
Singh GB, Khanna S, Raut SK, Sharma S, Sharma R, Khullar M
Therapeutic advances in cardiovascular disease11(5-6)147-154(2017 May-Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Methylation-mediated silencing of the DUSP-1 promoter does not appear to be associated with reduced expression, indicating the involvement of other factors in specific suppression of DUSP-1 in diabetes-associated cardiac hypertrophy.
Title: DUSP-1 gene expression is not regulated by promoter methylation in diabetes-associated cardiac hypertrophy.

miR-200c plays a role in diabetes-associated cardiac hypertrophy by modulating expression of DUSP-1.
Title: MicroRNA-200c modulates DUSP-1 expression in diabetes-induced cardiac hypertrophy.

our data show that HuR is a novel target of 15d-PGJ2 and reveal HuR-mediated pre-mRNA processing and mature mRNA stabilization as important regulatory steps in the 15d-PGJ2-induced expression of MKP-1.
Title: MAP kinase phosphatase-1 expression is regulated by 15-deoxy-?12,14-prostaglandin J2 via a HuR-dependent post-transcriptional mechanism.

MKP-1 in the hippocampus might be involved in the etiology of depression, and DNA methylation of MKP-1 gene promoter in the hippocampus did not related with the depression.
Title: Role of hippocampus mitogen-activated protein kinase phosphatase-1 mRNA expression and DNA methylation in the depression of the rats with chronic unpredicted stress.

protein phosphatase MKP-1 carries a negative regulation of c-fos gene transcription by dephosphorylation of ERK kinases that are a key signal component under the action of such stress reagent as anisomycin on the E1A + ras-transformed cells
Title: [Protein phosphatase MKP-1 participates in c-fos gene derepression under the action of stress factors on fibroblasts transformed with E1A and cHA-ras oncogenes].

The following Dusp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dusp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12821	NM_053769.3 Latest version!	Rattus norvegicus dual specificity phosphatase 1 (Dusp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dusp1 ( NM_053769.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446221.2 Rattus norvegicus dual specificity phosphatase 1 (Dusp1), mRNA.

Price & Availability↑

CloneID	ORa12821
Clone ID Related Accession (Same CDS sequence)	NM_053769.3
Accession Version	NM_053769.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Rattus norvegicus(Norway rat)
Product	dual specificity protein phosphatase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X84004.1, U02553.1 and DY314639.1. On Mar 27, 2007 this sequence version replaced NM_053769.2. Sequence Note: This RefSeq record was created from transcript sequence data from multiple strains because no single transcript from the same strain was available for the full length of the gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X84004.1, U02553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGATGG AGGTGGGCAT CCTGGACGCC GGGGGGCTGC GCGCGCTGCT GCGAGAGCGC 
GCCGCTCAGT GCCTGCTTCT GGATTGTCGC TCCTTCTTCG CCTTCAACGC CGGCCACATC 
GTGGGCTCAG TGAACGTGCG CTTCAGCACC ATCGTGCGGC GCCGCGCCAA GGGCGCCATG 
GGCCTGGAGC ATATCGTGCC GAACACCGAA CTGCGCGGCC GCCTGCTGGC CGGAGCCTAT 
CACGCCGTAG TGCTGCTGGA CGAACGCAGC GCCGCCCTGG ACGGCGCCAA GCGCGACGGC 
ACCCTGGCCC TGGCCGCGGG CGCGCTCTGC CGAGAAGCGC GCTCCACTCA AGTCTTCTTC 
CTCCAAGGAG GATATGAAGC GTTTTCGGCT TCCTGCCCTG AGCTGTGCAG CAAACAGTCC 
ACCCCCATGG GGCTCAGCCT CCCGCTGAGT ACTAGTGTGC CTGACAGTGC AGAATCCGGA 
TGCAGCTCCT GTAGCACCCC TCTCTACGAC CAGGGGGGCC CAGTGGAGAT CCTGTCCTTC 
CTGTACCTGG GCAGTGCTTA CCATGCTTCC CGGAAAGATA TGCTCGACGC CTTGGGTATC 
ACTGCTTTGA TCAACGTCTC GGCCAATTGT CCTAACCACT TTGAGGGTCA CTACCAGTAC 
AAGAGCATCC CTGTGGAGGA CAACCACAAG GCAGACATTA GCTCCTGGTT CAACGAGGCG 
ATTGACTTTA TAGACTCCAT CAAGGATGCT GGAGGAAGGG TGTTTGTGCA CTGCCAGGCC 
GGCATCTCCA GGTCAGCCAC CATCTGCCTT GCTTACCTCA TGAGGACTAA CCGAGTGAAG 
CTGGACGAGG CCTTTGAGTT CGTGAAGCAG AGGCGGAGTA TTATCTCCCC CAACTTCAGC 
TTCATGGGCC AGCTGCTGCA ATTTGAGTCC CAAGTACTGG CCCCTCACTG TTCTGCAGAA 
GCTGGGAGCC CGGCCATGGC TGTCCTTGAC CGGGGCACCT CTACTACAAC GGTCTTCAAC 
TTCCCTGTCT CCATCCCTGT TCACCCCACG AACAGTGCCC TGAACTACCT TCAAAGCCCC 
ATCACAACCT CTCCGAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446221.2
CDS	141..1244
Translation	MVMEVGILDAGGLRALLRERAAQCLLLDCRSFFAFNAGHIVGSVNVRFSTIVRRRAKGAMGLEHIVPNTELRGRLLAGAYHAVVLLDERSAALDGAKRDGTLALAAGALCREARSTQVFFLQGGYEAFSASCPELCSKQSTPMGLSLPLSTSVPDSAESGCSSCSTPLYDQGGPVEILSFLYLGSAYHASRKDMLDALGITALINVSANCPNHFEGHYQYKSIPVEDNHKADISSWFNEAIDFIDSIKDAGGRVFVHCQAGISRSATICLAYLMRTNRVKLDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHPTNSALNYLQSPITTSPSC

Target ORF information:

RefSeq Version	NM_053769.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dual specificity phosphatase 1 (Dusp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053769.3

ORF Insert Sequence:

ATGGTGATGG AGGTGGGCAT CCTGGACGCC GGGGGGCTGC GCGCGCTGCT GCGAGAGCGC 
GCCGCTCAGT GCCTGCTTCT GGATTGTCGC TCCTTCTTCG CCTTCAACGC CGGCCACATC 
GTGGGCTCAG TGAACGTGCG CTTCAGCACC ATCGTGCGGC GCCGCGCCAA GGGCGCCATG 
GGCCTGGAGC ATATCGTGCC GAACACCGAA CTGCGCGGCC GCCTGCTGGC CGGAGCCTAT 
CACGCCGTAG TGCTGCTGGA CGAACGCAGC GCCGCCCTGG ACGGCGCCAA GCGCGACGGC 
ACCCTGGCCC TGGCCGCGGG CGCGCTCTGC CGAGAAGCGC GCTCCACTCA AGTCTTCTTC 
CTCCAAGGAG GATATGAAGC GTTTTCGGCT TCCTGCCCTG AGCTGTGCAG CAAACAGTCC 
ACCCCCATGG GGCTCAGCCT CCCGCTGAGT ACTAGTGTGC CTGACAGTGC AGAATCCGGA 
TGCAGCTCCT GTAGCACCCC TCTCTACGAC CAGGGGGGCC CAGTGGAGAT CCTGTCCTTC 
CTGTACCTGG GCAGTGCTTA CCATGCTTCC CGGAAAGATA TGCTCGACGC CTTGGGTATC 
ACTGCTTTGA TCAACGTCTC GGCCAATTGT CCTAACCACT TTGAGGGTCA CTACCAGTAC 
AAGAGCATCC CTGTGGAGGA CAACCACAAG GCAGACATTA GCTCCTGGTT CAACGAGGCG 
ATTGACTTTA TAGACTCCAT CAAGGATGCT GGAGGAAGGG TGTTTGTGCA CTGCCAGGCC 
GGCATCTCCA GGTCAGCCAC CATCTGCCTT GCTTACCTCA TGAGGACTAA CCGAGTGAAG 
CTGGACGAGG CCTTTGAGTT CGTGAAGCAG AGGCGGAGTA TTATCTCCCC CAACTTCAGC 
TTCATGGGCC AGCTGCTGCA ATTTGAGTCC CAAGTACTGG CCCCTCACTG TTCTGCAGAA 
GCTGGGAGCC CGGCCATGGC TGTCCTTGAC CGGGGCACCT CTACTACAAC GGTCTTCAAC 
TTCCCTGTCT CCATCCCTGT TCACCCCACG AACAGTGCCC TGAACTACCT TCAAAGCCCC 
ATCACAACCT CTCCGAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reactive oxygen species overproduction and MAP kinase phosphatase-1 degradation are associated with gastroparesis in a streptozotocin-induced male diabetic rat model.
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society(2017 Nov)
Smiley R,Naik P,McCallum R,Showkat Ali M

MicroRNA-101 in the ventrolateral orbital cortex (VLO) modulates depressive-like behaviors in rats and targets dual-specificity phosphatase 1 (DUSP1).
Brain research166955-62(2017 Aug)
Zhao Y,Wang S,Chu Z,Dang Y,Zhu J,Su X

The Role of MKP-1 in Insulin-Induced Cardioprotection.
Cardiovascular drugs and therapy31(3)247-254(2017 Jun)
Webster I,Smith A,Lochner A,Huisamen B

DUSP-1 gene expression is not regulated by promoter methylation in diabetes-associated cardiac hypertrophy.
Therapeutic advances in cardiovascular disease11(5-6)147-154(2017 May-Jun)
Singh GB,Khanna S,Raut SK,Sharma S,Sharma R,Khullar M

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Dusp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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