Gne cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gne
Entrez Gene ID	114711
Full Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Synonyms	Uae1
General protein information	Preferred Names glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase Names bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase UDP-GlcNAc-2-epimerase/ManAc kinase UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q22
Summary	bifunctional enzyme, catalyzes the first two steps of biosynthesis of Neu5Ac (UDP-GlcNAc 2-epimerase and ManNAc kinase), precursor of sialic acids [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053765.2	NP_446217.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
XM_006238008.1	XP_006238070.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X3
XM_006238006.3	XP_006238068.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X1
XM_017593117.1	XP_017448606.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2
XM_006238009.3	XP_006238071.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	GNE	NP_001121699.1
Mus musculus (house mouse)	Gne	NP_056643.3
Rattus norvegicus (Norway rat)	Gne	NP_446217.1
	GNE	XP_001082113.2
Gallus gallus (chicken)	GNE	NP_001026603.2
Xenopus tropicalis (tropical clawed frog)	gne	NP_001072728.1
Canis lupus familiaris (dog)	GNE	XP_003431623.1
Danio rerio (zebrafish)	gne	NP_957177.1
Pan troglodytes (chimpanzee)	GNE	XP_003312121.1
Bos taurus (cattle)	GNE	NP_001178072.2

Gene Ontology
Bibliography

Related articles in PubMed

Biochemical characterization of the M712T-mutation of the UDP-N-acetylglucosamine 2-epimerase/N-acetyl-mannosaminekinase in hereditary inclusion body myopathy.
Weidemann W, Reinhardt A, Thate A, Horstkorte R
Neuromuscular disorders : NMD21(12)824-31(2011 Dec)

The key enzyme of sialic acid biosynthesis (GNE) promotes neurite outgrowth of PC12 cells.
Kontou M, Weidemann W, Bauer C, Reutter W, Horstkorte R
Neuroreport19(12)1239-42(2008 Aug)

Evidence for dynamic interplay of different oligomeric states of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase by biophysical methods.
Ghaderi D, Strauss HM, Reinke S, Cirak S, Reutter W, Lucka L, Hinderlich S
Journal of molecular biology369(3)746-58(2007 Jun)

The collapsin response mediator protein 1 (CRMP-1) and the promyelocytic leukemia zinc finger protein (PLZF) bind to UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE), the key enzyme of sialic acid biosynthesis.
Weidemann W, Stelzl U, Lisewski U, Bork K, Wanker EE, Hinderlich S, Horstkorte R
FEBS letters580(28-29)6649-54(2006 Dec)

Characterization of ligand binding to the bifunctional key enzyme in the sialic acid biosynthesis by NMR: I. Investigation of the UDP-GlcNAc 2-epimerase functionality.
Blume A, Benie AJ, Stolz F, Schmidt RR, Reutter W, Hinderlich S, Peters T
The Journal of biological chemistry279(53)55715-21(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

M712T mutation of GNE gene does not affect GNE oligomerization but affects the solubility and posttranslational modification of the GNE protein.
Title: Biochemical characterization of the M712T-mutation of the UDP-N-acetylglucosamine 2-epimerase/N-acetyl-mannosaminekinase in hereditary inclusion body myopathy.

Data show that overexpression of GNE induces neurite outgrowth in PC12 cells, but does not lead to an increased biosynthesis of sialic acid.
Title: The key enzyme of sialic acid biosynthesis (GNE) promotes neurite outgrowth of PC12 cells.

analysis of oligomeric states of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase by biophysical methods
Title: Evidence for dynamic interplay of different oligomeric states of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase by biophysical methods.

The collapsin response mediator protein 1 and the promyelocytic leukemia zinc finger protein interact with UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE).
Title: The collapsin response mediator protein 1 (CRMP-1) and the promyelocytic leukemia zinc finger protein (PLZF) bind to UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE), the key enzyme of sialic acid biosynthesis.

analysis of ligand binding to UDP-GlcNAc 2-epimerase by NMR
Title: Characterization of ligand binding to the bifunctional key enzyme in the sialic acid biosynthesis by NMR: I. Investigation of the UDP-GlcNAc 2-epimerase functionality.

The following Gne gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gne cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10557	NM_053765.2 Latest version!	Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa28108	XM_006238006.3 Latest version!	Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa28110	XM_006238008.1 Latest version!	Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa28110	XM_006238009.3 Latest version!	Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa61087	XM_017593117.1 Latest version!	Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gne ( NM_053765.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446217.1 Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), mRNA.

Price & Availability↑

CloneID	ORa10557
Clone ID Related Accession (Same CDS sequence)	NM_053765.2
Accession Version	NM_053765.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Rattus norvegicus(Norway rat)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC062011.1. On Dec 20, 2003 this sequence version replaced NM_053765.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062011.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAAGA ACGGGAATAA CCGGAAGCTT CGGGTTTGCG TTGCCACCTG CAACCGAGCC 
GATTACTCCA AACTGGCCCC CATCATGTTC GGCATTAAGA CGGAGCCTGC GTTCTTCGAG 
CTCGACGTGG TGGTGCTGGG CTCTCACCTG ATCGACGACT ACGGAAACAC ATACCGCATG 
ATTGAGCAGG ACGACTTTGA CATCAACACC AGGCTACACA CGATTGTTAG AGGGGAAGAC 
GAAGCAGCCA TGGTAGAGTC AGTGGGCCTA GCGCTAGTGA AGCTACCGGA TGTCCTCAAC 
CGCCTGAAGC CTGACATCAT GATTGTTCAC GGAGACCGAT TTGACGCCCT CGCTCTGGCT 
ACATCTGCTG CCCTGATGAA CATCCGCATC CTTCACATTG AAGGAGGAGA GGTCAGCGGG 
ACTATTGATG ACTCTATCAG ACACGCCATA ACAAAACTGG CTCACTACCA CGTGTGCTGC 
ACCAGGAGTG CAGAGCAACA CCTGATCTCC ATGTGTGAGG ACCACGACCG CATCCTTTTG 
GCTGGCTGCC CTTCCTATGA CAAACTGCTC TCAGCCAAGA ATAAAGACTA TATGAGCATC 
ATTCGGATGT GGCTAGGTGA TGATGTAAAA TGTAAAGATT ACATTGTTGC CCTGCAACAC 
CCGGTGACCA CCGACATTAA GCATTCCATA AAGATGTTCG AACTGACACT GGATGCTCTT 
ATCTCATTTA ACAAGAGGAC CCTAGTTCTG TTTCCAAATA TCGATGCAGG CAGCAAGGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATC GAGCATCACC CCAATTTCCG CGCAGTCAAG 
CACGTCCCGT TTGACCAGTT CATTCAGCTG GTCGCCCACG CTGGCTGCAT GATTGGGAAT 
AGCAGCTGTG GAGTGCGTGA GGTTGGCGCC TTTGGAACCC CTGTGATCAA CCTGGGCACG 
CGGCAGATAG GAAGAGAAAC GGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TACAAGCACT ACACCTCCAG TTCGGTAAAC AGTACCCTTG CTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTAAAGTTTC TCAAATCCAT CGACCTTCAA 
GAGCCACTAC AGAAGAAATT CTGCTTCCCT CCCGTGAAGG AGAACATCTC TCAGGATATT 
GACCATATCC TCGAAACTCT GAGTGCCTTG GCTGTTGATC TCGGGGGGAC GAATCTGAGA 
GTGGCGATAG TTAGCATGAA GGGTGAAATA GTTAAGAAGT ACACCCAGTT CAATCCTAAA 
ACCTATGAGG AAAGGATTAG TCTAATCCTG CAGATGTGTG TGGAAGCGGC AGCAGAAGCC 
GTGAAGCTCA ATTGCAGAAT TCTGGGAGTA GGCATCTCCA CAGGTGGCCG TGTGAATCCC 
CAGGAAGGAG TTGTGCTGCA CTCGACCAAG CTGATACAGG AGTGGAACTC TGTGGACCTC 
AGGACACCAC TCTCCGACAC CCTGCATCTC CCCGTGTGGG TGGACAACGA CGGCAACTGC 
GCTGCCATGG CGGAGAGGAA GTTTGGCCAA GGAAAAGGAC AGGAGAACTT TGTGACGCTC 
ATCACAGGGA CAGGGATCGG TGGGGGAATC ATCCACCAGC ACGAGCTGAT CCACGGCAGC 
TCCTTCTGTG CGGCAGAGCT TGGCCACCTC GTGGTGTCTC TGGATGGTCC TGACTGCTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCCTAC GCCTCTGGAA TGGCCTTGCA GAGGGAAGCA 
AAGAAGCTCC ACGACGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGACGAA 
GCTGTGGGCG CCCTCCATCT CATCCAAGCC GCCAAGCTGG GCAACGTGAA GGCCCAGAGC 
ATCTTACGGA CAGCTGGAAC TGCTTTGGGA CTCGGAGTTG TGAATATCCT CCACACTATG 
AATCCTTCCC TGGTGATCCT GTCTGGAGTC CTGGCTAGTC ACTACATCCA CATTGTGAGG 
GACGTCATCC GCCAGCAAGC CCTGTCCTCC GTGCAGGATG TGGATGTAGT GGTTTCAGAC 
TTGGTTGACC CGGCCCTGCT TGGTGCGGCC AGCATGGTTC TGGACTACAC GACCCGCAGG 
ATCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446217.1
CDS	340..2508
Translation	MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPAFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKCKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERISLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPQEGVVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAAMAERKFGQGKGQENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVRDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIH

Target ORF information:

RefSeq Version	NM_053765.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053765.2

ORF Insert Sequence:

ATGGAGAAGA ACGGGAATAA CCGGAAGCTT CGGGTTTGCG TTGCCACCTG CAACCGAGCC 
GATTACTCCA AACTGGCCCC CATCATGTTC GGCATTAAGA CGGAGCCTGC GTTCTTCGAG 
CTCGACGTGG TGGTGCTGGG CTCTCACCTG ATCGACGACT ACGGAAACAC ATACCGCATG 
ATTGAGCAGG ACGACTTTGA CATCAACACC AGGCTACACA CGATTGTTAG AGGGGAAGAC 
GAAGCAGCCA TGGTAGAGTC AGTGGGCCTA GCGCTAGTGA AGCTACCGGA TGTCCTCAAC 
CGCCTGAAGC CTGACATCAT GATTGTTCAC GGAGACCGAT TTGACGCCCT CGCTCTGGCT 
ACATCTGCTG CCCTGATGAA CATCCGCATC CTTCACATTG AAGGAGGAGA GGTCAGCGGG 
ACTATTGATG ACTCTATCAG ACACGCCATA ACAAAACTGG CTCACTACCA CGTGTGCTGC 
ACCAGGAGTG CAGAGCAACA CCTGATCTCC ATGTGTGAGG ACCACGACCG CATCCTTTTG 
GCTGGCTGCC CTTCCTATGA CAAACTGCTC TCAGCCAAGA ATAAAGACTA TATGAGCATC 
ATTCGGATGT GGCTAGGTGA TGATGTAAAA TGTAAAGATT ACATTGTTGC CCTGCAACAC 
CCGGTGACCA CCGACATTAA GCATTCCATA AAGATGTTCG AACTGACACT GGATGCTCTT 
ATCTCATTTA ACAAGAGGAC CCTAGTTCTG TTTCCAAATA TCGATGCAGG CAGCAAGGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATC GAGCATCACC CCAATTTCCG CGCAGTCAAG 
CACGTCCCGT TTGACCAGTT CATTCAGCTG GTCGCCCACG CTGGCTGCAT GATTGGGAAT 
AGCAGCTGTG GAGTGCGTGA GGTTGGCGCC TTTGGAACCC CTGTGATCAA CCTGGGCACG 
CGGCAGATAG GAAGAGAAAC GGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TACAAGCACT ACACCTCCAG TTCGGTAAAC AGTACCCTTG CTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTAAAGTTTC TCAAATCCAT CGACCTTCAA 
GAGCCACTAC AGAAGAAATT CTGCTTCCCT CCCGTGAAGG AGAACATCTC TCAGGATATT 
GACCATATCC TCGAAACTCT GAGTGCCTTG GCTGTTGATC TCGGGGGGAC GAATCTGAGA 
GTGGCGATAG TTAGCATGAA GGGTGAAATA GTTAAGAAGT ACACCCAGTT CAATCCTAAA 
ACCTATGAGG AAAGGATTAG TCTAATCCTG CAGATGTGTG TGGAAGCGGC AGCAGAAGCC 
GTGAAGCTCA ATTGCAGAAT TCTGGGAGTA GGCATCTCCA CAGGTGGCCG TGTGAATCCC 
CAGGAAGGAG TTGTGCTGCA CTCGACCAAG CTGATACAGG AGTGGAACTC TGTGGACCTC 
AGGACACCAC TCTCCGACAC CCTGCATCTC CCCGTGTGGG TGGACAACGA CGGCAACTGC 
GCTGCCATGG CGGAGAGGAA GTTTGGCCAA GGAAAAGGAC AGGAGAACTT TGTGACGCTC 
ATCACAGGGA CAGGGATCGG TGGGGGAATC ATCCACCAGC ACGAGCTGAT CCACGGCAGC 
TCCTTCTGTG CGGCAGAGCT TGGCCACCTC GTGGTGTCTC TGGATGGTCC TGACTGCTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCCTAC GCCTCTGGAA TGGCCTTGCA GAGGGAAGCA 
AAGAAGCTCC ACGACGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGACGAA 
GCTGTGGGCG CCCTCCATCT CATCCAAGCC GCCAAGCTGG GCAACGTGAA GGCCCAGAGC 
ATCTTACGGA CAGCTGGAAC TGCTTTGGGA CTCGGAGTTG TGAATATCCT CCACACTATG 
AATCCTTCCC TGGTGATCCT GTCTGGAGTC CTGGCTAGTC ACTACATCCA CATTGTGAGG 
GACGTCATCC GCCAGCAAGC CCTGTCCTCC GTGCAGGATG TGGATGTAGT GGTTTCAGAC 
TTGGTTGACC CGGCCCTGCT TGGTGCGGCC AGCATGGTTC TGGACTACAC GACCCGCAGG 
ATCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gne ( XM_006238006.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006238068.1 Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa28108
Clone ID Related Accession (Same CDS sequence)	XM_006238006.3
Accession Version	XM_006238006.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC ACATGCCTCT CCACAGGGAG CAGAGCCACG CAGGACCTCA TGAACTCTAT 
TTTAAAAAAC TCTCAAGTAA AAAAAAGCAA GTCATGGAGA AGAACGGGAA TAACCGGAAG 
CTTCGGGTTT GCGTTGCCAC CTGCAACCGA GCCGATTACT CCAAACTGGC CCCCATCATG 
TTCGGCATTA AGACGGAGCC TGCGTTCTTC GAGCTCGACG TGGTGGTGCT GGGCTCTCAC 
CTGATCGACG ACTACGGAAA CACATACCGC ATGATTGAGC AGGACGACTT TGACATCAAC 
ACCAGGCTAC ACACGATTGT TAGAGGGGAA GACGAAGCAG CCATGGTAGA GTCAGTGGGC 
CTAGCGCTAG TGAAGCTACC GGATGTCCTC AACCGCCTGA AGCCTGACAT CATGATTGTT 
CACGGAGACC GATTTGACGC CCTCGCTCTG GCTACATCTG CTGCCCTGAT GAACATCCGC 
ATCCTTCACA TTGAAGGAGG AGAGGTCAGT GGGACTATTG ATGACTCTAT CAGACACGCC 
ATAACAAAAC TGGCTCACTA CCACGTGTGC TGCACCAGGA GTGCAGAGCA ACACCTGATC 
TCCATGTGTG AGGACCACGA CCGCATCCTT TTGGCCGGCT GCCCTTCCTA TGACAAACTG 
CTCTCAGCCA AGAATAAAGA CTATATGAGC ATCATTCGGA TGTGGCTAGG TGATGATGTA 
AAATGTAAAG ATTACATTGT TGCCCTGCAA CACCCGGTGA CCACCGACAT TAAGCATTCC 
ATAAAGATGT TCGAACTGAC ACTGGATGCT CTTATCTCAT TTAACAAGAG GACCCTAGTT 
CTGTTTCCAA ATATCGATGC AGGCAGCAAG GAGATGGTTC GAGTGATGCG GAAGAAGGGC 
ATCGAGCATC ACCCCAATTT CCGCGCAGTC AAGCACGTCC CGTTTGACCA GTTCATTCAG 
CTGGTCGCCC ACGCTGGCTG CATGATTGGG AATAGCAGCT GTGGAGTGCG TGAGGTTGGC 
GCCTTTGGAA CCCCTGTGAT CAACCTGGGC ACGCGGCAGA TAGGAAGAGA AACGGGGGAG 
AATGTTCTTC ATGTCCGGGA TGCTGACACC CAAGACAAAA TATTACAAGC ACTACACCTC 
CAGTTCGGTA AACAGTACCC TTGCTCAAAG ATATATGGGG ATGGAAATGC TGTTCCAAGG 
ATTTTAAAGT TTCTCAAATC CATCGACCTT CAAGAGCCAC TACAGAAGAA ATTCTGCTTC 
CCTCCCGTGA AGGAGAACAT CTCTCAGGAT ATTGACCATA TCCTCGAAAC TCTGAGTGCC 
TTGGCTGTTG ATCTCGGGGG GACGAATCTG AGAGTGGCGA TAGTTAGCAT GAAGGGTGAA 
ATAGTTAAGA AGTACACCCA GTTCAATCCT AAAACCTATG AGGAAAGGAT TAGTCTAATC 
CTGCAGATGT GTGTGGAAGC GGCAGCAGAA GCCGTGAAGC TCAATTGCAG AATTCTGGGA 
GTAGGCATCT CCACAGGTGG CCGTGTGAAT CCCCAGGAAG GAGTTGTGCT GCACTCGACC 
AAGCTGATAC AGGAGTGGAA CTCTGTGGAC CTCAGGACAC CACTCTCCGA CACCCTGCAT 
CTCCCCGTGT GGGTGGACAA CGACGGCAAC TGCGCTGCCA TGGCGGAGAG GAAGTTTGGC 
CAAGGAAAAG GACAGGAGAA CTTTGTGACG CTCATCACAG GGACAGGGAT CGGTGGGGGA 
ATCATCCACC AGCACGAGCT GATCCACGGC AGCTCCTTCT GTGCGGCAGA GCTTGGCCAC 
CTCGTGGTGT CTCTGGATGG TCCTGACTGC TCCTGTGGAA GCCATGGGTG CATTGAAGCC 
TACGCCTCTG GAATGGCCTT GCAGAGGGAA GCAAAGAAGC TCCACGACGA GGACCTGCTC 
TTGGTGGAAG GGATGTCAGT GCCAAAAGAC GAAGCTGTGG GCGCCCTCCA TCTCATCCAA 
GCCGCCAAGC TGGGCAACGT GAAGGCCCAG AGCATCTTAC GGACAGCTGG AACTGCTTTG 
GGACTCGGAG TTGTGAATAT CCTCCACACT ATGAATCCTT CCCTGGTGAT CCTGTCTGGA 
GTCCTGGCTA GTCACTACAT CCACATTGTG AGGGACGTCA TCCGCCAGCA AGCCCTGTCC 
TCCGTGCAGG ATGTGGATGT AGTGGTTTCA GACTTGGTTG ACCCGGCCCT GCTTGGTGCG 
GCCAGCATGG TTCTGGACTA CACGACCCGC AGGATCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006238068.1
CDS	63..2324
Translation	METHMPLHREQSHAGPHELYFKKLSSKKKQVMEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPAFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKCKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERISLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPQEGVVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAAMAERKFGQGKGQENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVRDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIH

Target ORF information:

RefSeq Version	XM_006238006.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238006.3

ORF Insert Sequence:

ATGGAAACAC ACATGCCTCT CCACAGGGAG CAGAGCCACG CAGGACCTCA TGAACTCTAT 
TTTAAAAAAC TCTCAAGTAA AAAAAAGCAA GTCATGGAGA AGAACGGGAA TAACCGGAAG 
CTTCGGGTTT GCGTTGCCAC CTGCAACCGA GCCGATTACT CCAAACTGGC CCCCATCATG 
TTCGGCATTA AGACGGAGCC TGCGTTCTTC GAGCTCGACG TGGTGGTGCT GGGCTCTCAC 
CTGATCGACG ACTACGGAAA CACATACCGC ATGATTGAGC AGGACGACTT TGACATCAAC 
ACCAGGCTAC ACACGATTGT TAGAGGGGAA GACGAAGCAG CCATGGTAGA GTCAGTGGGC 
CTAGCGCTAG TGAAGCTACC GGATGTCCTC AACCGCCTGA AGCCTGACAT CATGATTGTT 
CACGGAGACC GATTTGACGC CCTCGCTCTG GCTACATCTG CTGCCCTGAT GAACATCCGC 
ATCCTTCACA TTGAAGGAGG AGAGGTCAGT GGGACTATTG ATGACTCTAT CAGACACGCC 
ATAACAAAAC TGGCTCACTA CCACGTGTGC TGCACCAGGA GTGCAGAGCA ACACCTGATC 
TCCATGTGTG AGGACCACGA CCGCATCCTT TTGGCCGGCT GCCCTTCCTA TGACAAACTG 
CTCTCAGCCA AGAATAAAGA CTATATGAGC ATCATTCGGA TGTGGCTAGG TGATGATGTA 
AAATGTAAAG ATTACATTGT TGCCCTGCAA CACCCGGTGA CCACCGACAT TAAGCATTCC 
ATAAAGATGT TCGAACTGAC ACTGGATGCT CTTATCTCAT TTAACAAGAG GACCCTAGTT 
CTGTTTCCAA ATATCGATGC AGGCAGCAAG GAGATGGTTC GAGTGATGCG GAAGAAGGGC 
ATCGAGCATC ACCCCAATTT CCGCGCAGTC AAGCACGTCC CGTTTGACCA GTTCATTCAG 
CTGGTCGCCC ACGCTGGCTG CATGATTGGG AATAGCAGCT GTGGAGTGCG TGAGGTTGGC 
GCCTTTGGAA CCCCTGTGAT CAACCTGGGC ACGCGGCAGA TAGGAAGAGA AACGGGGGAG 
AATGTTCTTC ATGTCCGGGA TGCTGACACC CAAGACAAAA TATTACAAGC ACTACACCTC 
CAGTTCGGTA AACAGTACCC TTGCTCAAAG ATATATGGGG ATGGAAATGC TGTTCCAAGG 
ATTTTAAAGT TTCTCAAATC CATCGACCTT CAAGAGCCAC TACAGAAGAA ATTCTGCTTC 
CCTCCCGTGA AGGAGAACAT CTCTCAGGAT ATTGACCATA TCCTCGAAAC TCTGAGTGCC 
TTGGCTGTTG ATCTCGGGGG GACGAATCTG AGAGTGGCGA TAGTTAGCAT GAAGGGTGAA 
ATAGTTAAGA AGTACACCCA GTTCAATCCT AAAACCTATG AGGAAAGGAT TAGTCTAATC 
CTGCAGATGT GTGTGGAAGC GGCAGCAGAA GCCGTGAAGC TCAATTGCAG AATTCTGGGA 
GTAGGCATCT CCACAGGTGG CCGTGTGAAT CCCCAGGAAG GAGTTGTGCT GCACTCGACC 
AAGCTGATAC AGGAGTGGAA CTCTGTGGAC CTCAGGACAC CACTCTCCGA CACCCTGCAT 
CTCCCCGTGT GGGTGGACAA CGACGGCAAC TGCGCTGCCA TGGCGGAGAG GAAGTTTGGC 
CAAGGAAAAG GACAGGAGAA CTTTGTGACG CTCATCACAG GGACAGGGAT CGGTGGGGGA 
ATCATCCACC AGCACGAGCT GATCCACGGC AGCTCCTTCT GTGCGGCAGA GCTTGGCCAC 
CTCGTGGTGT CTCTGGATGG TCCTGACTGC TCCTGTGGAA GCCATGGGTG CATTGAAGCC 
TACGCCTCTG GAATGGCCTT GCAGAGGGAA GCAAAGAAGC TCCACGACGA GGACCTGCTC 
TTGGTGGAAG GGATGTCAGT GCCAAAAGAC GAAGCTGTGG GCGCCCTCCA TCTCATCCAA 
GCCGCCAAGC TGGGCAACGT GAAGGCCCAG AGCATCTTAC GGACAGCTGG AACTGCTTTG 
GGACTCGGAG TTGTGAATAT CCTCCACACT ATGAATCCTT CCCTGGTGAT CCTGTCTGGA 
GTCCTGGCTA GTCACTACAT CCACATTGTG AGGGACGTCA TCCGCCAGCA AGCCCTGTCC 
TCCGTGCAGG ATGTGGATGT AGTGGTTTCA GACTTGGTTG ACCCGGCCCT GCTTGGTGCG 
GCCAGCATGG TTCTGGACTA CACGACCCGC AGGATCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gne ( XM_006238008.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006238070.1 Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa28110
Clone ID Related Accession (Same CDS sequence)	XM_006238008.1 , XM_006238009.3
Accession Version	XM_006238008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA ACGGGAATAA CCGGAAGCTT CGGGTTTGCG TTGCCACCTG CAACCGAGCC 
GATTACTCCA AACTGGCCCC CATCATGTTC GGCATTAAGA CGGAGCCTGC GTTCTTCGAG 
CTCGACGTGG TGGTGCTGGG CTCTCACCTG ATCGACGACT ACGGAAACAC ATACCGCATG 
ATTGAGCAGG ACGACTTTGA CATCAACACC AGGCTACACA CGATTGTTAG AGGGGAAGAC 
GAAGCAGCCA TGGTAGAGTC AGTGGGCCTA GCGCTAGTGA AGCTACCGGA TGTCCTCAAC 
CGCCTGAAGC CTGACATCAT GATTGTTCAC GGAGACCGAT TTGACGCCCT CGCTCTGGCT 
ACATCTGCTG CCCTGATGAA CATCCGCATC CTTCACATTG AAGGAGGAGA GGTCAGTGGG 
ACTATTGATG ACTCTATCAG ACACGCCATA ACAAAACTGG CTCACTACCA CGTGTGCTGC 
ACCAGGAGTG CAGAGCAACA CCTGATCTCC ATGTGTGAGG ACCACGACCG CATCCTTTTG 
GCCGGCTGCC CTTCCTATGA CAAACTGCTC TCAGCCAAGA ATAAAGACTA TATGAGCATC 
ATTCGGATGT GGCTAGGTGA TGATGTAAAA TGTAAAGATT ACATTGTTGC CCTGCAACAC 
CCGGTGACCA CCGACATTAA GCATTCCATA AAGATGTTCG AACTGACACT GGATGCTCTT 
ATCTCATTTA ACAAGAGGAC CCTAGTTCTG TTTCCAAATA TCGATGCAGG CAGCAAGGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATC GAGCATCACC CCAATTTCCG CGCAGTCAAG 
CACGTCCCGT TTGACCAGTT CATTCAGCTG GTCGCCCACG CTGGCTGCAT GATTGGGAAT 
AGCAGCTGTG GAGTGCGTGA GGTTGGCGCC TTTGGAACCC CTGTGATCAA CCTGGGCACG 
CGGCAGATAG GAAGAGAAAC GGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TACAAGCACT ACACCTCCAG TTCGGTAAAC AGTACCCTTG CTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTAAAGTTTC TCAAATCCAT CGACCTTCAA 
GAGCCACTAC AGAAGAAATT CTGCTTCCCT CCCGTGAAGG AGAACATCTC TCAGGATATT 
GACCATATCC TCGAAACTCT GAGTGCCTTG GCTGTTGATC TCGGGGGGAC GAATCTGAGA 
GTGGCGATAG TTAGCATGAA GGGTGAAATA GTTAAGAAGT ACACCCAGTT CAATCCTAAA 
ACCTATGAGG AAAGGATTAG TCTAATCCTG CAGATGTGTG TGGAAGCGGC AGCAGAAGCC 
GTGAAGCTCA ATTGCAGAAT TCTGGGAGTA GGCATCTCCA CAGGTGGCCG TGTGAATCCC 
CAGGAAGGAG TTGTGCTGCA CTCGACCAAG CTGATACAGG AGTGGAACTC TGTGGACCTC 
AGGACACCAC TCTCCGACAC CCTGCATCTC CCCGTGTGGG TGGACAACGA CGGCAACTGC 
GCTGCCATGG CGGAGAGGAA GTTTGGCCAA GGAAAAGGAC AGGAGAACTT TGTGACGCTC 
ATCACAGGGA CAGGGATCGG TGGGGGAATC ATCCACCAGC ACGAGCTGAT CCACGGCAGC 
TCCTTCTGTG CGGCAGAGCT TGGCCACCTC GTGGTGTCTC TGGATGGTCC TGACTGCTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCCTAC GCCTCTGGAA TGGCCTTGCA GAGGGAAGCA 
AAGAAGCTCC ACGACGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGACGAA 
GCTGTGGGCG CCCTCCATCT CATCCAAGCC GCCAAGCTGG GCAACGTGAA GGCCCAGAGC 
ATCTTACGGA CAGCTGGAAC TGCTTTGGGA CTCGGAGTTG TGAATATCCT CCACACTATG 
AATCCTTCCC TGGTGATCCT GTCTGGAGTC CTGGCTAGTC ACTACATCCA CATTGTGAGG 
GACGTCATCC GCCAGCAAGC CCTGTCCTCC GTGCAGGATG TGGATGTAGT GGTTTCAGAC 
TTGGTTGACC CGGCCCTGCT TGGTGCGGCC AGCATGGTTC TGGACTACAC GACCCGCAGG 
ATCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006238070.1
CDS	124..2292
Translation	MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPAFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKCKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERISLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPQEGVVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAAMAERKFGQGKGQENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVRDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIH

Target ORF information:

RefSeq Version	XM_006238008.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238008.1

ORF Insert Sequence:

ATGGAGAAGA ACGGGAATAA CCGGAAGCTT CGGGTTTGCG TTGCCACCTG CAACCGAGCC 
GATTACTCCA AACTGGCCCC CATCATGTTC GGCATTAAGA CGGAGCCTGC GTTCTTCGAG 
CTCGACGTGG TGGTGCTGGG CTCTCACCTG ATCGACGACT ACGGAAACAC ATACCGCATG 
ATTGAGCAGG ACGACTTTGA CATCAACACC AGGCTACACA CGATTGTTAG AGGGGAAGAC 
GAAGCAGCCA TGGTAGAGTC AGTGGGCCTA GCGCTAGTGA AGCTACCGGA TGTCCTCAAC 
CGCCTGAAGC CTGACATCAT GATTGTTCAC GGAGACCGAT TTGACGCCCT CGCTCTGGCT 
ACATCTGCTG CCCTGATGAA CATCCGCATC CTTCACATTG AAGGAGGAGA GGTCAGTGGG 
ACTATTGATG ACTCTATCAG ACACGCCATA ACAAAACTGG CTCACTACCA CGTGTGCTGC 
ACCAGGAGTG CAGAGCAACA CCTGATCTCC ATGTGTGAGG ACCACGACCG CATCCTTTTG 
GCCGGCTGCC CTTCCTATGA CAAACTGCTC TCAGCCAAGA ATAAAGACTA TATGAGCATC 
ATTCGGATGT GGCTAGGTGA TGATGTAAAA TGTAAAGATT ACATTGTTGC CCTGCAACAC 
CCGGTGACCA CCGACATTAA GCATTCCATA AAGATGTTCG AACTGACACT GGATGCTCTT 
ATCTCATTTA ACAAGAGGAC CCTAGTTCTG TTTCCAAATA TCGATGCAGG CAGCAAGGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATC GAGCATCACC CCAATTTCCG CGCAGTCAAG 
CACGTCCCGT TTGACCAGTT CATTCAGCTG GTCGCCCACG CTGGCTGCAT GATTGGGAAT 
AGCAGCTGTG GAGTGCGTGA GGTTGGCGCC TTTGGAACCC CTGTGATCAA CCTGGGCACG 
CGGCAGATAG GAAGAGAAAC GGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TACAAGCACT ACACCTCCAG TTCGGTAAAC AGTACCCTTG CTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTAAAGTTTC TCAAATCCAT CGACCTTCAA 
GAGCCACTAC AGAAGAAATT CTGCTTCCCT CCCGTGAAGG AGAACATCTC TCAGGATATT 
GACCATATCC TCGAAACTCT GAGTGCCTTG GCTGTTGATC TCGGGGGGAC GAATCTGAGA 
GTGGCGATAG TTAGCATGAA GGGTGAAATA GTTAAGAAGT ACACCCAGTT CAATCCTAAA 
ACCTATGAGG AAAGGATTAG TCTAATCCTG CAGATGTGTG TGGAAGCGGC AGCAGAAGCC 
GTGAAGCTCA ATTGCAGAAT TCTGGGAGTA GGCATCTCCA CAGGTGGCCG TGTGAATCCC 
CAGGAAGGAG TTGTGCTGCA CTCGACCAAG CTGATACAGG AGTGGAACTC TGTGGACCTC 
AGGACACCAC TCTCCGACAC CCTGCATCTC CCCGTGTGGG TGGACAACGA CGGCAACTGC 
GCTGCCATGG CGGAGAGGAA GTTTGGCCAA GGAAAAGGAC AGGAGAACTT TGTGACGCTC 
ATCACAGGGA CAGGGATCGG TGGGGGAATC ATCCACCAGC ACGAGCTGAT CCACGGCAGC 
TCCTTCTGTG CGGCAGAGCT TGGCCACCTC GTGGTGTCTC TGGATGGTCC TGACTGCTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCCTAC GCCTCTGGAA TGGCCTTGCA GAGGGAAGCA 
AAGAAGCTCC ACGACGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGACGAA 
GCTGTGGGCG CCCTCCATCT CATCCAAGCC GCCAAGCTGG GCAACGTGAA GGCCCAGAGC 
ATCTTACGGA CAGCTGGAAC TGCTTTGGGA CTCGGAGTTG TGAATATCCT CCACACTATG 
AATCCTTCCC TGGTGATCCT GTCTGGAGTC CTGGCTAGTC ACTACATCCA CATTGTGAGG 
GACGTCATCC GCCAGCAAGC CCTGTCCTCC GTGCAGGATG TGGATGTAGT GGTTTCAGAC 
TTGGTTGACC CGGCCCTGCT TGGTGCGGCC AGCATGGTTC TGGACTACAC GACCCGCAGG 
ATCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gne ( XM_006238009.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006238071.1 Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa28110
Clone ID Related Accession (Same CDS sequence)	XM_006238008.1 , XM_006238009.3
Accession Version	XM_006238009.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA ACGGGAATAA CCGGAAGCTT CGGGTTTGCG TTGCCACCTG CAACCGAGCC 
GATTACTCCA AACTGGCCCC CATCATGTTC GGCATTAAGA CGGAGCCTGC GTTCTTCGAG 
CTCGACGTGG TGGTGCTGGG CTCTCACCTG ATCGACGACT ACGGAAACAC ATACCGCATG 
ATTGAGCAGG ACGACTTTGA CATCAACACC AGGCTACACA CGATTGTTAG AGGGGAAGAC 
GAAGCAGCCA TGGTAGAGTC AGTGGGCCTA GCGCTAGTGA AGCTACCGGA TGTCCTCAAC 
CGCCTGAAGC CTGACATCAT GATTGTTCAC GGAGACCGAT TTGACGCCCT CGCTCTGGCT 
ACATCTGCTG CCCTGATGAA CATCCGCATC CTTCACATTG AAGGAGGAGA GGTCAGTGGG 
ACTATTGATG ACTCTATCAG ACACGCCATA ACAAAACTGG CTCACTACCA CGTGTGCTGC 
ACCAGGAGTG CAGAGCAACA CCTGATCTCC ATGTGTGAGG ACCACGACCG CATCCTTTTG 
GCCGGCTGCC CTTCCTATGA CAAACTGCTC TCAGCCAAGA ATAAAGACTA TATGAGCATC 
ATTCGGATGT GGCTAGGTGA TGATGTAAAA TGTAAAGATT ACATTGTTGC CCTGCAACAC 
CCGGTGACCA CCGACATTAA GCATTCCATA AAGATGTTCG AACTGACACT GGATGCTCTT 
ATCTCATTTA ACAAGAGGAC CCTAGTTCTG TTTCCAAATA TCGATGCAGG CAGCAAGGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATC GAGCATCACC CCAATTTCCG CGCAGTCAAG 
CACGTCCCGT TTGACCAGTT CATTCAGCTG GTCGCCCACG CTGGCTGCAT GATTGGGAAT 
AGCAGCTGTG GAGTGCGTGA GGTTGGCGCC TTTGGAACCC CTGTGATCAA CCTGGGCACG 
CGGCAGATAG GAAGAGAAAC GGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TACAAGCACT ACACCTCCAG TTCGGTAAAC AGTACCCTTG CTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTAAAGTTTC TCAAATCCAT CGACCTTCAA 
GAGCCACTAC AGAAGAAATT CTGCTTCCCT CCCGTGAAGG AGAACATCTC TCAGGATATT 
GACCATATCC TCGAAACTCT GAGTGCCTTG GCTGTTGATC TCGGGGGGAC GAATCTGAGA 
GTGGCGATAG TTAGCATGAA GGGTGAAATA GTTAAGAAGT ACACCCAGTT CAATCCTAAA 
ACCTATGAGG AAAGGATTAG TCTAATCCTG CAGATGTGTG TGGAAGCGGC AGCAGAAGCC 
GTGAAGCTCA ATTGCAGAAT TCTGGGAGTA GGCATCTCCA CAGGTGGCCG TGTGAATCCC 
CAGGAAGGAG TTGTGCTGCA CTCGACCAAG CTGATACAGG AGTGGAACTC TGTGGACCTC 
AGGACACCAC TCTCCGACAC CCTGCATCTC CCCGTGTGGG TGGACAACGA CGGCAACTGC 
GCTGCCATGG CGGAGAGGAA GTTTGGCCAA GGAAAAGGAC AGGAGAACTT TGTGACGCTC 
ATCACAGGGA CAGGGATCGG TGGGGGAATC ATCCACCAGC ACGAGCTGAT CCACGGCAGC 
TCCTTCTGTG CGGCAGAGCT TGGCCACCTC GTGGTGTCTC TGGATGGTCC TGACTGCTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCCTAC GCCTCTGGAA TGGCCTTGCA GAGGGAAGCA 
AAGAAGCTCC ACGACGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGACGAA 
GCTGTGGGCG CCCTCCATCT CATCCAAGCC GCCAAGCTGG GCAACGTGAA GGCCCAGAGC 
ATCTTACGGA CAGCTGGAAC TGCTTTGGGA CTCGGAGTTG TGAATATCCT CCACACTATG 
AATCCTTCCC TGGTGATCCT GTCTGGAGTC CTGGCTAGTC ACTACATCCA CATTGTGAGG 
GACGTCATCC GCCAGCAAGC CCTGTCCTCC GTGCAGGATG TGGATGTAGT GGTTTCAGAC 
TTGGTTGACC CGGCCCTGCT TGGTGCGGCC AGCATGGTTC TGGACTACAC GACCCGCAGG 
ATCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006238071.1
CDS	501..2669
Translation	MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPAFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKCKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERISLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPQEGVVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAAMAERKFGQGKGQENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVRDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIH

Target ORF information:

RefSeq Version	XM_006238009.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238009.3

ORF Insert Sequence:

ATGGAGAAGA ACGGGAATAA CCGGAAGCTT CGGGTTTGCG TTGCCACCTG CAACCGAGCC 
GATTACTCCA AACTGGCCCC CATCATGTTC GGCATTAAGA CGGAGCCTGC GTTCTTCGAG 
CTCGACGTGG TGGTGCTGGG CTCTCACCTG ATCGACGACT ACGGAAACAC ATACCGCATG 
ATTGAGCAGG ACGACTTTGA CATCAACACC AGGCTACACA CGATTGTTAG AGGGGAAGAC 
GAAGCAGCCA TGGTAGAGTC AGTGGGCCTA GCGCTAGTGA AGCTACCGGA TGTCCTCAAC 
CGCCTGAAGC CTGACATCAT GATTGTTCAC GGAGACCGAT TTGACGCCCT CGCTCTGGCT 
ACATCTGCTG CCCTGATGAA CATCCGCATC CTTCACATTG AAGGAGGAGA GGTCAGTGGG 
ACTATTGATG ACTCTATCAG ACACGCCATA ACAAAACTGG CTCACTACCA CGTGTGCTGC 
ACCAGGAGTG CAGAGCAACA CCTGATCTCC ATGTGTGAGG ACCACGACCG CATCCTTTTG 
GCCGGCTGCC CTTCCTATGA CAAACTGCTC TCAGCCAAGA ATAAAGACTA TATGAGCATC 
ATTCGGATGT GGCTAGGTGA TGATGTAAAA TGTAAAGATT ACATTGTTGC CCTGCAACAC 
CCGGTGACCA CCGACATTAA GCATTCCATA AAGATGTTCG AACTGACACT GGATGCTCTT 
ATCTCATTTA ACAAGAGGAC CCTAGTTCTG TTTCCAAATA TCGATGCAGG CAGCAAGGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATC GAGCATCACC CCAATTTCCG CGCAGTCAAG 
CACGTCCCGT TTGACCAGTT CATTCAGCTG GTCGCCCACG CTGGCTGCAT GATTGGGAAT 
AGCAGCTGTG GAGTGCGTGA GGTTGGCGCC TTTGGAACCC CTGTGATCAA CCTGGGCACG 
CGGCAGATAG GAAGAGAAAC GGGGGAGAAT GTTCTTCATG TCCGGGATGC TGACACCCAA 
GACAAAATAT TACAAGCACT ACACCTCCAG TTCGGTAAAC AGTACCCTTG CTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTAAAGTTTC TCAAATCCAT CGACCTTCAA 
GAGCCACTAC AGAAGAAATT CTGCTTCCCT CCCGTGAAGG AGAACATCTC TCAGGATATT 
GACCATATCC TCGAAACTCT GAGTGCCTTG GCTGTTGATC TCGGGGGGAC GAATCTGAGA 
GTGGCGATAG TTAGCATGAA GGGTGAAATA GTTAAGAAGT ACACCCAGTT CAATCCTAAA 
ACCTATGAGG AAAGGATTAG TCTAATCCTG CAGATGTGTG TGGAAGCGGC AGCAGAAGCC 
GTGAAGCTCA ATTGCAGAAT TCTGGGAGTA GGCATCTCCA CAGGTGGCCG TGTGAATCCC 
CAGGAAGGAG TTGTGCTGCA CTCGACCAAG CTGATACAGG AGTGGAACTC TGTGGACCTC 
AGGACACCAC TCTCCGACAC CCTGCATCTC CCCGTGTGGG TGGACAACGA CGGCAACTGC 
GCTGCCATGG CGGAGAGGAA GTTTGGCCAA GGAAAAGGAC AGGAGAACTT TGTGACGCTC 
ATCACAGGGA CAGGGATCGG TGGGGGAATC ATCCACCAGC ACGAGCTGAT CCACGGCAGC 
TCCTTCTGTG CGGCAGAGCT TGGCCACCTC GTGGTGTCTC TGGATGGTCC TGACTGCTCC 
TGTGGAAGCC ATGGGTGCAT TGAAGCCTAC GCCTCTGGAA TGGCCTTGCA GAGGGAAGCA 
AAGAAGCTCC ACGACGAGGA CCTGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGACGAA 
GCTGTGGGCG CCCTCCATCT CATCCAAGCC GCCAAGCTGG GCAACGTGAA GGCCCAGAGC 
ATCTTACGGA CAGCTGGAAC TGCTTTGGGA CTCGGAGTTG TGAATATCCT CCACACTATG 
AATCCTTCCC TGGTGATCCT GTCTGGAGTC CTGGCTAGTC ACTACATCCA CATTGTGAGG 
GACGTCATCC GCCAGCAAGC CCTGTCCTCC GTGCAGGATG TGGATGTAGT GGTTTCAGAC 
TTGGTTGACC CGGCCCTGCT TGGTGCGGCC AGCATGGTTC TGGACTACAC GACCCGCAGG 
ATCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gne ( XM_017593117.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448606.1 Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa61087
Clone ID Related Accession (Same CDS sequence)	XM_017593117.1
Accession Version	XM_017593117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAATCC TTCCCAGAAG AGGTGACACT TGGCTGACTT CTGAAGCCTG GGAACTCTAT 
TTTAAAAAAC TCTCAAGTAA AAAAAAGCAA GTCATGGAGA AGAACGGGAA TAACCGGAAG 
CTTCGGGTTT GCGTTGCCAC CTGCAACCGA GCCGATTACT CCAAACTGGC CCCCATCATG 
TTCGGCATTA AGACGGAGCC TGCGTTCTTC GAGCTCGACG TGGTGGTGCT GGGCTCTCAC 
CTGATCGACG ACTACGGAAA CACATACCGC ATGATTGAGC AGGACGACTT TGACATCAAC 
ACCAGGCTAC ACACGATTGT TAGAGGGGAA GACGAAGCAG CCATGGTAGA GTCAGTGGGC 
CTAGCGCTAG TGAAGCTACC GGATGTCCTC AACCGCCTGA AGCCTGACAT CATGATTGTT 
CACGGAGACC GATTTGACGC CCTCGCTCTG GCTACATCTG CTGCCCTGAT GAACATCCGC 
ATCCTTCACA TTGAAGGAGG AGAGGTCAGT GGGACTATTG ATGACTCTAT CAGACACGCC 
ATAACAAAAC TGGCTCACTA CCACGTGTGC TGCACCAGGA GTGCAGAGCA ACACCTGATC 
TCCATGTGTG AGGACCACGA CCGCATCCTT TTGGCCGGCT GCCCTTCCTA TGACAAACTG 
CTCTCAGCCA AGAATAAAGA CTATATGAGC ATCATTCGGA TGTGGCTAGG TGATGATGTA 
AAATGTAAAG ATTACATTGT TGCCCTGCAA CACCCGGTGA CCACCGACAT TAAGCATTCC 
ATAAAGATGT TCGAACTGAC ACTGGATGCT CTTATCTCAT TTAACAAGAG GACCCTAGTT 
CTGTTTCCAA ATATCGATGC AGGCAGCAAG GAGATGGTTC GAGTGATGCG GAAGAAGGGC 
ATCGAGCATC ACCCCAATTT CCGCGCAGTC AAGCACGTCC CGTTTGACCA GTTCATTCAG 
CTGGTCGCCC ACGCTGGCTG CATGATTGGG AATAGCAGCT GTGGAGTGCG TGAGGTTGGC 
GCCTTTGGAA CCCCTGTGAT CAACCTGGGC ACGCGGCAGA TAGGAAGAGA AACGGGGGAG 
AATGTTCTTC ATGTCCGGGA TGCTGACACC CAAGACAAAA TATTACAAGC ACTACACCTC 
CAGTTCGGTA AACAGTACCC TTGCTCAAAG ATATATGGGG ATGGAAATGC TGTTCCAAGG 
ATTTTAAAGT TTCTCAAATC CATCGACCTT CAAGAGCCAC TACAGAAGAA ATTCTGCTTC 
CCTCCCGTGA AGGAGAACAT CTCTCAGGAT ATTGACCATA TCCTCGAAAC TCTGAGTGCC 
TTGGCTGTTG ATCTCGGGGG GACGAATCTG AGAGTGGCGA TAGTTAGCAT GAAGGGTGAA 
ATAGTTAAGA AGTACACCCA GTTCAATCCT AAAACCTATG AGGAAAGGAT TAGTCTAATC 
CTGCAGATGT GTGTGGAAGC GGCAGCAGAA GCCGTGAAGC TCAATTGCAG AATTCTGGGA 
GTAGGCATCT CCACAGGTGG CCGTGTGAAT CCCCAGGAAG GAGTTGTGCT GCACTCGACC 
AAGCTGATAC AGGAGTGGAA CTCTGTGGAC CTCAGGACAC CACTCTCCGA CACCCTGCAT 
CTCCCCGTGT GGGTGGACAA CGACGGCAAC TGCGCTGCCA TGGCGGAGAG GAAGTTTGGC 
CAAGGAAAAG GACAGGAGAA CTTTGTGACG CTCATCACAG GGACAGGGAT CGGTGGGGGA 
ATCATCCACC AGCACGAGCT GATCCACGGC AGCTCCTTCT GTGCGGCAGA GCTTGGCCAC 
CTCGTGGTGT CTCTGGATGG TCCTGACTGC TCCTGTGGAA GCCATGGGTG CATTGAAGCC 
TACGCCTCTG GAATGGCCTT GCAGAGGGAA GCAAAGAAGC TCCACGACGA GGACCTGCTC 
TTGGTGGAAG GGATGTCAGT GCCAAAAGAC GAAGCTGTGG GCGCCCTCCA TCTCATCCAA 
GCCGCCAAGC TGGGCAACGT GAAGGCCCAG AGCATCTTAC GGACAGCTGG AACTGCTTTG 
GGACTCGGAG TTGTGAATAT CCTCCACACT ATGAATCCTT CCCTGGTGAT CCTGTCTGGA 
GTCCTGGCTA GTCACTACAT CCACATTGTG AGGGACGTCA TCCGCCAGCA AGCCCTGTCC 
TCCGTGCAGG ATGTGGATGT AGTGGTTTCA GACTTGGTTG ACCCGGCCCT GCTTGGTGCG 
GCCAGCATGG TTCTGGACTA CACGACCCGC AGGATCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448606.1
CDS	1107..3368
Translation	MGILPRRGDTWLTSEAWELYFKKLSSKKKQVMEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPAFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKCKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERISLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPQEGVVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAAMAERKFGQGKGQENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVRDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIH

Target ORF information:

RefSeq Version	XM_017593117.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593117.1

ORF Insert Sequence:

ATGGGAATCC TTCCCAGAAG AGGTGACACT TGGCTGACTT CTGAAGCCTG GGAACTCTAT 
TTTAAAAAAC TCTCAAGTAA AAAAAAGCAA GTCATGGAGA AGAACGGGAA TAACCGGAAG 
CTTCGGGTTT GCGTTGCCAC CTGCAACCGA GCCGATTACT CCAAACTGGC CCCCATCATG 
TTCGGCATTA AGACGGAGCC TGCGTTCTTC GAGCTCGACG TGGTGGTGCT GGGCTCTCAC 
CTGATCGACG ACTACGGAAA CACATACCGC ATGATTGAGC AGGACGACTT TGACATCAAC 
ACCAGGCTAC ACACGATTGT TAGAGGGGAA GACGAAGCAG CCATGGTAGA GTCAGTGGGC 
CTAGCGCTAG TGAAGCTACC GGATGTCCTC AACCGCCTGA AGCCTGACAT CATGATTGTT 
CACGGAGACC GATTTGACGC CCTCGCTCTG GCTACATCTG CTGCCCTGAT GAACATCCGC 
ATCCTTCACA TTGAAGGAGG AGAGGTCAGT GGGACTATTG ATGACTCTAT CAGACACGCC 
ATAACAAAAC TGGCTCACTA CCACGTGTGC TGCACCAGGA GTGCAGAGCA ACACCTGATC 
TCCATGTGTG AGGACCACGA CCGCATCCTT TTGGCCGGCT GCCCTTCCTA TGACAAACTG 
CTCTCAGCCA AGAATAAAGA CTATATGAGC ATCATTCGGA TGTGGCTAGG TGATGATGTA 
AAATGTAAAG ATTACATTGT TGCCCTGCAA CACCCGGTGA CCACCGACAT TAAGCATTCC 
ATAAAGATGT TCGAACTGAC ACTGGATGCT CTTATCTCAT TTAACAAGAG GACCCTAGTT 
CTGTTTCCAA ATATCGATGC AGGCAGCAAG GAGATGGTTC GAGTGATGCG GAAGAAGGGC 
ATCGAGCATC ACCCCAATTT CCGCGCAGTC AAGCACGTCC CGTTTGACCA GTTCATTCAG 
CTGGTCGCCC ACGCTGGCTG CATGATTGGG AATAGCAGCT GTGGAGTGCG TGAGGTTGGC 
GCCTTTGGAA CCCCTGTGAT CAACCTGGGC ACGCGGCAGA TAGGAAGAGA AACGGGGGAG 
AATGTTCTTC ATGTCCGGGA TGCTGACACC CAAGACAAAA TATTACAAGC ACTACACCTC 
CAGTTCGGTA AACAGTACCC TTGCTCAAAG ATATATGGGG ATGGAAATGC TGTTCCAAGG 
ATTTTAAAGT TTCTCAAATC CATCGACCTT CAAGAGCCAC TACAGAAGAA ATTCTGCTTC 
CCTCCCGTGA AGGAGAACAT CTCTCAGGAT ATTGACCATA TCCTCGAAAC TCTGAGTGCC 
TTGGCTGTTG ATCTCGGGGG GACGAATCTG AGAGTGGCGA TAGTTAGCAT GAAGGGTGAA 
ATAGTTAAGA AGTACACCCA GTTCAATCCT AAAACCTATG AGGAAAGGAT TAGTCTAATC 
CTGCAGATGT GTGTGGAAGC GGCAGCAGAA GCCGTGAAGC TCAATTGCAG AATTCTGGGA 
GTAGGCATCT CCACAGGTGG CCGTGTGAAT CCCCAGGAAG GAGTTGTGCT GCACTCGACC 
AAGCTGATAC AGGAGTGGAA CTCTGTGGAC CTCAGGACAC CACTCTCCGA CACCCTGCAT 
CTCCCCGTGT GGGTGGACAA CGACGGCAAC TGCGCTGCCA TGGCGGAGAG GAAGTTTGGC 
CAAGGAAAAG GACAGGAGAA CTTTGTGACG CTCATCACAG GGACAGGGAT CGGTGGGGGA 
ATCATCCACC AGCACGAGCT GATCCACGGC AGCTCCTTCT GTGCGGCAGA GCTTGGCCAC 
CTCGTGGTGT CTCTGGATGG TCCTGACTGC TCCTGTGGAA GCCATGGGTG CATTGAAGCC 
TACGCCTCTG GAATGGCCTT GCAGAGGGAA GCAAAGAAGC TCCACGACGA GGACCTGCTC 
TTGGTGGAAG GGATGTCAGT GCCAAAAGAC GAAGCTGTGG GCGCCCTCCA TCTCATCCAA 
GCCGCCAAGC TGGGCAACGT GAAGGCCCAG AGCATCTTAC GGACAGCTGG AACTGCTTTG 
GGACTCGGAG TTGTGAATAT CCTCCACACT ATGAATCCTT CCCTGGTGAT CCTGTCTGGA 
GTCCTGGCTA GTCACTACAT CCACATTGTG AGGGACGTCA TCCGCCAGCA AGCCCTGTCC 
TCCGTGCAGG ATGTGGATGT AGTGGTTTCA GACTTGGTTG ACCCGGCCCT GCTTGGTGCG 
GCCAGCATGG TTCTGGACTA CACGACCCGC AGGATCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biochemical characterization of the M712T-mutation of the UDP-N-acetylglucosamine 2-epimerase/N-acetyl-mannosaminekinase in hereditary inclusion body myopathy.
Neuromuscular disorders : NMD21(12)824-31(2011 Dec)
Weidemann W,Reinhardt A,Thate A,Horstkorte R

The key enzyme of sialic acid biosynthesis (GNE) promotes neurite outgrowth of PC12 cells.
Neuroreport19(12)1239-42(2008 Aug)
Kontou M,Weidemann W,Bauer C,Reutter W,Horstkorte R

Evidence for dynamic interplay of different oligomeric states of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase by biophysical methods.
Journal of molecular biology369(3)746-58(2007 Jun)
Ghaderi D,Strauss HM,Reinke S,Cirak S,Reutter W,Lucka L,Hinderlich S

The collapsin response mediator protein 1 (CRMP-1) and the promyelocytic leukemia zinc finger protein (PLZF) bind to UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE), the key enzyme of sialic acid biosynthesis.
FEBS letters580(28-29)6649-54(2006 Dec)
Weidemann W,Stelzl U,Lisewski U,Bork K,Wanker EE,Hinderlich S,Horstkorte R

Characterization of ligand binding to the bifunctional key enzyme in the sialic acid biosynthesis by NMR: I. Investigation of the UDP-GlcNAc 2-epimerase functionality.
The Journal of biological chemistry279(53)55715-21(2004 Dec)
Blume A,Benie AJ,Stolz F,Schmidt RR,Reutter W,Hinderlich S,Peters T

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Gne cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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