Pi4k2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pi4k2a
Entrez Gene ID	114554
Full Name	phosphatidylinositol 4-kinase type 2 alpha
Synonyms	Pi4KII
General protein information	Preferred Names phosphatidylinositol 4-kinase type 2 alpha Names phosphatidylinositol 4-kinase type 2-alpha 55 kDa type II phosphatidylinositol 4-kinase phosphatidylinositol 4-kinase type II phosphatidylinositol 4-kinase type II-alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q54
Summary	catalyzes the conversion of PtdIns to PtdIns 4-phosphate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053735.1	NP_446187.1	phosphatidylinositol 4-kinase type 2-alpha

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PI4K2A	XP_543953.2
Rattus norvegicus (Norway rat)	Pi4k2a	NP_446187.1
Caenorhabditis elegans (roundworm)	ZC8.6	NP_508849.3
Drosophila melanogaster (fruit fly)	Pi4KIIalpha	NP_996154.1
Xenopus tropicalis (tropical clawed frog)	pi4k2a	NP_001263612.1
Homo sapiens (human)	PI4K2A	NP_060895.1
Pan troglodytes (chimpanzee)	PI4K2A	XP_507965.2
Bos taurus (cattle)	PI4K2A	NP_001093786.1
Mus musculus (house mouse)	Pi4k2a	NP_663476.1
Gallus gallus (chicken)	PI4K2A	XP_423069.1
Danio rerio (zebrafish)	pi4k2a	NP_998523.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003121	XP_562889.4

Gene Ontology
Bibliography

Related articles in PubMed

The crystal structure of the phosphatidylinositol 4-kinase II?.
Baumlova A, Chalupska D, R??ycki B, Jovic M, Wisniewski E, Klima M, Dubankova A, Kloer DP, Nencka R, Balla T, Boura E
EMBO reports15(10)1085-92(2014 Oct)

Molecular insights into the membrane-associated phosphatidylinositol 4-kinase II?.
Zhou Q, Li J, Yu H, Zhai Y, Gao Z, Liu Y, Pang X, Zhang L, Schulten K, Sun F, Chen C
Nature communications53552(2014 Mar)

The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse.
Larimore J, Tornieri K, Ryder PV, Gokhale A, Zlatic SA, Craige B, Lee JD, Talbot K, Pare JF, Smith Y, Faundez V
Molecular biology of the cell22(24)4854-67(2011 Dec)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Phosphatidylinositol-4-kinase type II alpha contains an AP-3-sorting motif and a kinase domain that are both required for endosome traffic.
Craige B, Salazar G, Faundez V
Molecular biology of the cell19(4)1415-26(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PI4KIIalpha plays a role in the exocytotic process downstream of Ca(2+) signaling in antigen-mediated mast cell activation.
Title: The role of phosphatidylinositol 4-kinase type IIalpha in degranulation of RBL-2H3 cells.

PI4KIIalpha is present on adaptor protein (AP) -3 organelles where it regulates AP-3 function
Title: Phosphatidylinositol-4-kinase type II alpha is a component of adaptor protein-3-derived vesicles.

This enzyme is a downstream target of neuronal calcium sensor-1 in enhancing exocytosis in neuroendocrine cells
Title: Phosphatidylinositol 4-OH kinase is a downstream target of neuronal calcium sensor-1 in enhancing exocytosis in neuroendocrine cells.

defined the minimal catalytic domain and identified conserved amino acid residues required for catalysis
Title: Analysis of the catalytic domain of phosphatidylinositol 4-kinase type II.

The following Pi4k2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pi4k2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42544	NM_053735.1 Latest version!	Rattus norvegicus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pi4k2a ( NM_053735.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446187.1 Rattus norvegicus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), mRNA.

Price & Availability↑

CloneID	ORa42544
Clone ID Related Accession (Same CDS sequence)	NM_053735.1
Accession Version	NM_053735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylinositol 4-kinase type 2-alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY029199.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY029199.1 [ECO:0000331] ##Evidence-Data-END##

ATGGACGAGA CGAGCCCGCT AGTGTCCCCC GAGCGGGCCC AACCCCCTGA GTACACCTTC 
CCGTCGGTCT CGGGAGCTCA CTTTCCGCAG GTACCGGGGG GCGCGGTCCG CGTAGCGGCG 
GCCGGCTCCG GCCCGTCCCC GCCGTGCTCT CCGGGCCACG ACCGGGAGCG GCAGCCCCTG 
CTGGACCGGG CCCGGGGCGC GGCGGCGCAG GGTCAGACCC ACACGGTGGC GGCGCAGGCC 
CAGGCCCTGG CCGCCCAGGC GGCCGTGGCG GTGCACGCCG TTCAGACCCA CCGCGAGCGA 
AACGACTTCC CGGAGGACCC CGAGTTCGAG GTGGTGGTGC GGCAGGCCGA GATAGCCATC 
GAGTGCAGCA TCTATCCCGA GCGCATCTAC CAGGGCTCCA GTGGAAGCTA CTTCGTCAAG 
GACTCTCAGG GGAGGATCAT TGCTGTCTTC AAACCCAAGA ATGAAGAGCC ATACGGGAAC 
CTTAACCCTA AGTGGACCAA ATGGCTTCAG AAGCTGTGCT GCCCCTGCTG CTTTGGCCGA 
GATTGCCTTG TTCTCAACCA GGGCTATCTC TCAGAAGCAG GGGCTAGCCT GGTGGACCAA 
AAACTGGAAC TCAACATTGT ACCCCGTACA AAGGTAGTAT ACCTGGCCAG TGAAACCTTC 
AACTATAGTG CCATTGACCG AGTAAAGTCC AGGGGCAAGC GACTTGCACT AGAGAAAGTG 
CCAAAAGTTG GGCAGCGGTT TAACCGCATT GGGCTACCAC CAAAGGTCGG GTCATTCCAG 
CTCTTTGTTG AAGGCTACAA AGATGCAGAC TATTGGCTAC GGCGTTTTGA AGCAGAACCT 
CTTCCCGAGA ACACGAACCG ACAGCTACTA TTGCAGTTTG AGCGGTTGGT GGTCCTGGAC 
TACATCATCC GCAACACTGA CCGAGGCAAT GACAACTGGT TGATCAAATA TGACTATCCG 
ATGGATAATC CTAACTGTCG GGACACAGAC TGGGTGATGG TGAGGGAGCC TGTTATCAAG 
GTGGCCGCCA TAGACAATGG ACTGGCTTTC CCACTGAAGC ACCCTGACTC CTGGAGGGCA 
TATCCTTTTT ACTGGGCCTG GTTGCCACAG GCTAAAGTCC CATTCTCTCA GGAGATCAAA 
GATTTGATTC TTCCAAAGAT TTCAGACCCT AACTTCGTCA AGGACTTGGA GGAGGACCTA 
TATGAACTCT TCAAGAAAGA TCCTGGTTTC GACAGGGGCC AGTTCCATAA GCAGATTGCT 
GTCATGAGAG GTCAGATCCT AAATTTGACC CAGGCCCTAA AAGACAATAA GAGTCCCCTG 
CACCTCGTCC AGATGCCACC CGTGATTGTT GAGACGGCCC GTTCTCATCA GCGGTCTTCA 
AGTGAATCCT ACACACAGAG CTTCCAGAGT CGGAAGCCCT TCTTTTCATG GTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446187.1
CDS	58..1494
Translation	MDETSPLVSPERAQPPEYTFPSVSGAHFPQVPGGAVRVAAAGSGPSPPCSPGHDRERQPLLDRARGAAAQGQTHTVAAQAQALAAQAAVAVHAVQTHRERNDFPEDPEFEVVVRQAEIAIECSIYPERIYQGSSGSYFVKDSQGRIIAVFKPKNEEPYGNLNPKWTKWLQKLCCPCCFGRDCLVLNQGYLSEAGASLVDQKLELNIVPRTKVVYLASETFNYSAIDRVKSRGKRLALEKVPKVGQRFNRIGLPPKVGSFQLFVEGYKDADYWLRRFEAEPLPENTNRQLLLQFERLVVLDYIIRNTDRGNDNWLIKYDYPMDNPNCRDTDWVMVREPVIKVAAIDNGLAFPLKHPDSWRAYPFYWAWLPQAKVPFSQEIKDLILPKISDPNFVKDLEEDLYELFKKDPGFDRGQFHKQIAVMRGQILNLTQALKDNKSPLHLVQMPPVIVETARSHQRSSSESYTQSFQSRKPFFSWW

Target ORF information:

RefSeq Version	NM_053735.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphatidylinositol 4-kinase type 2 alpha (Pi4k2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053735.1

ORF Insert Sequence:

ATGGACGAGA CGAGCCCGCT AGTGTCCCCC GAGCGGGCCC AACCCCCTGA GTACACCTTC 
CCGTCGGTCT CGGGAGCTCA CTTTCCGCAG GTACCGGGGG GCGCGGTCCG CGTAGCGGCG 
GCCGGCTCCG GCCCGTCCCC GCCGTGCTCT CCGGGCCACG ACCGGGAGCG GCAGCCCCTG 
CTGGACCGGG CCCGGGGCGC GGCGGCGCAG GGTCAGACCC ACACGGTGGC GGCGCAGGCC 
CAGGCCCTGG CCGCCCAGGC GGCCGTGGCG GTGCACGCCG TTCAGACCCA CCGCGAGCGA 
AACGACTTCC CGGAGGACCC CGAGTTCGAG GTGGTGGTGC GGCAGGCCGA GATAGCCATC 
GAGTGCAGCA TCTATCCCGA GCGCATCTAC CAGGGCTCCA GTGGAAGCTA CTTCGTCAAG 
GACTCTCAGG GGAGGATCAT TGCTGTCTTC AAACCCAAGA ATGAAGAGCC ATACGGGAAC 
CTTAACCCTA AGTGGACCAA ATGGCTTCAG AAGCTGTGCT GCCCCTGCTG CTTTGGCCGA 
GATTGCCTTG TTCTCAACCA GGGCTATCTC TCAGAAGCAG GGGCTAGCCT GGTGGACCAA 
AAACTGGAAC TCAACATTGT ACCCCGTACA AAGGTAGTAT ACCTGGCCAG TGAAACCTTC 
AACTATAGTG CCATTGACCG AGTAAAGTCC AGGGGCAAGC GACTTGCACT AGAGAAAGTG 
CCAAAAGTTG GGCAGCGGTT TAACCGCATT GGGCTACCAC CAAAGGTCGG GTCATTCCAG 
CTCTTTGTTG AAGGCTACAA AGATGCAGAC TATTGGCTAC GGCGTTTTGA AGCAGAACCT 
CTTCCCGAGA ACACGAACCG ACAGCTACTA TTGCAGTTTG AGCGGTTGGT GGTCCTGGAC 
TACATCATCC GCAACACTGA CCGAGGCAAT GACAACTGGT TGATCAAATA TGACTATCCG 
ATGGATAATC CTAACTGTCG GGACACAGAC TGGGTGATGG TGAGGGAGCC TGTTATCAAG 
GTGGCCGCCA TAGACAATGG ACTGGCTTTC CCACTGAAGC ACCCTGACTC CTGGAGGGCA 
TATCCTTTTT ACTGGGCCTG GTTGCCACAG GCTAAAGTCC CATTCTCTCA GGAGATCAAA 
GATTTGATTC TTCCAAAGAT TTCAGACCCT AACTTCGTCA AGGACTTGGA GGAGGACCTA 
TATGAACTCT TCAAGAAAGA TCCTGGTTTC GACAGGGGCC AGTTCCATAA GCAGATTGCT 
GTCATGAGAG GTCAGATCCT AAATTTGACC CAGGCCCTAA AAGACAATAA GAGTCCCCTG 
CACCTCGTCC AGATGCCACC CGTGATTGTT GAGACGGCCC GTTCTCATCA GCGGTCTTCA 
AGTGAATCCT ACACACAGAG CTTCCAGAGT CGGAAGCCCT TCTTTTCATG GTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The crystal structure of the phosphatidylinositol 4-kinase II?.
EMBO reports15(10)1085-92(2014 Oct)
Baumlova A,Chalupska D,R??ycki B,Jovic M,Wisniewski E,Klima M,Dubankova A,Kloer DP,Nencka R,Balla T,Boura E

Molecular insights into the membrane-associated phosphatidylinositol 4-kinase II?.
Nature communications53552(2014 Mar)
Zhou Q,Li J,Yu H,Zhai Y,Gao Z,Liu Y,Pang X,Zhang L,Schulten K,Sun F,Chen C

The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse.
Molecular biology of the cell22(24)4854-67(2011 Dec)
Larimore J,Tornieri K,Ryder PV,Gokhale A,Zlatic SA,Craige B,Lee JD,Talbot K,Pare JF,Smith Y,Faundez V

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Phosphatidylinositol-4-kinase type II alpha contains an AP-3-sorting motif and a kinase domain that are both required for endosome traffic.
Molecular biology of the cell19(4)1415-26(2008 Apr)
Craige B,Salazar G,Faundez V

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Pi4k2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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