Ncf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ncf1
Entrez Gene ID	114553
Full Name	neutrophil cytosolic factor 1
Synonyms	Ncf-1,p47phox
General protein information	Preferred Names neutrophil cytosolic factor 1 Names neutrophil cytosolic factor 1 neutrophil cytosol factor 1 phagocyte oxidase 47 kDa
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q12
Summary	involved in the regulation of arthritis severity in rats [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053734.2	NP_446186.2	neutrophil cytosolic factor 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NCF1	NP_000256.4
Canis lupus familiaris (dog)	NCF1	XP_849574.2
Mus musculus (house mouse)	Ncf1	NP_001272966.1
Rattus norvegicus (Norway rat)	Ncf1	NP_446186.2
Xenopus tropicalis (tropical clawed frog)	ncf1	NP_001106375.1
	NCF1	XP_001084055.1
Gallus gallus (chicken)	NCF1	NP_001025880.1
Pan troglodytes (chimpanzee)	NCF1	XP_001152639.1
Bos taurus (cattle)	NCF1	NP_776544.1
Danio rerio (zebrafish)	ncf1	NP_001025242.1

Gene Ontology
Bibliography

Related articles in PubMed

Activation of p47phox as a Mechanism of Bupivacaine-Induced Burst Production of Reactive Oxygen Species and Neural Toxicity.
Li YJ, Zhao W, Yu XJ, Li FX, Liu ZT, Li L, Xu SY
Oxidative medicine and cellular longevity20178539026(2017)

Exercise Training Attenuates Upregulation of p47(phox) and p67(phox) in Hearts of Diabetic Rats.
Sharma NM, Rabeler B, Zheng H, Raichlin E, Patel KP
Oxidative medicine and cellular longevity20165868913(2016)

Melatonin nephroprotective action in Zucker diabetic fatty rats involves its inhibitory effect on NADPH oxidase.
Winiarska K, Dzik JM, Labudda M, Focht D, Sierakowski B, Owczarek A, Komorowski L, Bielecki W
Journal of pineal research60(1)109-17(2016 Jan)

Cadmium induces matrix metalloproteinase-9 expression via ROS-dependent EGFR, NF-?B, and AP-1 pathways in human endothelial cells.
Lian S, Xia Y, Khoi PN, Ung TT, Yoon HJ, Kim NH, Kim KK, Jung YD
Toxicology338104-16(2015 Dec)

NADPH oxidase-derived production of reactive oxygen species is involved in learning and memory impairments in 16-month-old female rats.
Kan H, Hu W, Wang Y, Wu W, Yin Y, Liang Y, Wang C, Huang D, Li W
Molecular medicine reports12(3)4546-53(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Exercise Training attenuates the increased expression of p47(phox) and p67(phox) in the hearts of diabetic rats which could be an underlying mechanism for improving intracardiac matrix and thus cardiac function and prevent cardiac remodeling in diabetic cardiomyopathy.
Title: Exercise Training Attenuates Upregulation of p47(phox) and p67(phox) in Hearts of Diabetic Rats.

it is concluded that: (i) melatonin beneficial action against diabetic nephropathy involves attenuation of the excessive activity of Nox; and (ii) the mechanism of melatonin inhibitory effect on Nox is based on the mitigation of expression and membrane translocation of its regulatory subunits.
Title: Melatonin nephroprotective action in Zucker diabetic fatty rats involves its inhibitory effect on NADPH oxidase.

NOX2 and p47phox-derived production of reactive oxygen species is involved in learning and memory impairments in 16-month-old female rats.
Title: NADPH oxidase-derived production of reactive oxygen species is involved in learning and memory impairments in 16-month-old female rats.

The levels of cytosolic p67Phox, p47Phox, and p40Phox were increased in membrane fractions following traumatic brain injury.
Title: A time course of NADPH-oxidase up-regulation and endothelial nitric oxide synthase activation in the hippocampus following neurotrauma.

Exposure to dopamine (DA) substantially increased levels of astrocytic NADPH oxidase subunits and induced p47(phox) phosphorylation.
Title: Elevated dopamine induces minimal hepatic encephalopathy by activation of astrocytic NADPH oxidase and astrocytic protein tyrosine nitration.

The following Ncf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13248	NM_053734.2 Latest version!	Rattus norvegicus neutrophil cytosolic factor 1 (Ncf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ncf1 ( NM_053734.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446186.2 Rattus norvegicus neutrophil cytosolic factor 1 (Ncf1), mRNA.

Price & Availability↑

CloneID	ORa13248
Clone ID Related Accession (Same CDS sequence)	NM_053734.2
Accession Version	NM_053734.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	neutrophil cytosolic factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061810.1. On Dec 20, 2003 this sequence version replaced NM_053734.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061810.1, AF547392.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGACA CCTTCATTCG CCACATCGCC CTCCTGGGCT TCGAGAAACG CTTCGTCCCC 
AGCCAACACT ATGTGTACAT GTTCCTGGTT AAGTGGCAGG ACCTGTCGGA GAAGGTGGTC 
TACAGAAAAT TCACCGAGAT CTACGAGTTC CATAAAATGT TAAAGGAGAT GTTCCCCATT 
GAGGCCGGTG AGATCCACAC AGAAAACAGA GTCATCCCTC ACCTCCCAGC TCCCAGGTGG 
TATGATGGGC AGCGTGCAGC GGAGAGCCGC CAGGGAACGC TCACCGAGTA CTTCAACAGC 
CTCATGGGAC TGCCCGTGAA GATCTCCCGC TGCCCACACC TCTTGAACTT CTTCAAAGTG 
CGGCCCGATG ACCTGAAGCT GCCCAATGAC AGCCAGGTGA AGAAGCCAGA GACATACCTG 
ACGGCCAAAG ATGGCAAGAA TAATGTAGCT GACATCACGG GTCCCATCAT CCTTCAGACC 
TATCGGGCCA TCGCTGACTA CGAGAAGGGT TCCAAAACAG AGATGACCGT GGCGACGGGA 
GATGTGGTGG ATGTCGTAGA GAAAAGCGAG AGTGGCTGGT GGTTTTGCCA GATGAAGACA 
AAACGAGGTT GGGTCCCTGC ATCCTATTTG GAGCCCCTTG ACAGCCCTGA TGAGGCAGAG 
GACCCCGATC CCAACTACGC AGGTGAACCG TATGTAACCA TCAAAGCGTA CGCTGCTGTT 
GAAGAGGATG AGGTGTCCCT GTCTGAGGGT GAAGCCATCG AGGTCATTCA TAAGCTCCTA 
GATGGCTGGT GGGTGGTCAG GAAAGGGGAC ATCACCGGCT ACTTCCCATC CATGTATCTG 
CAGAAGGCTG GGGAGGAGAT AACCCAGGCC CAGCGACAGA TTAGAAGCCG CGGGGCACCA 
CCTCGCAGGT CGACCATCCG CAATGCACAG AGCATCCACC AGCGTTCTCG GAAGCGCCTC 
AGCCAGGACA CCTATCGCCG CAACAGCGTC CGATTCCTGC AGCAGCGCAG ACGCCCGGCG 
CGACCTGGGC CGCAGAGCCC TGACTCAAAG GACAATCCAT CGACTCCGCG CGCCAAACCA 
CAGCCTGCGG TGCCTCCGAG ACCCAGCTCG GACCTCATCC TGCACCGCTG CACAGAGAGC 
ACCAAGAGGA AACTGACGTC CGCCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446186.2
CDS	15..1184
Translation	MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRKFTEIYEFHKMLKEMFPIEAGEIHTENRVIPHLPAPRWYDGQRAAESRQGTLTEYFNSLMGLPVKISRCPHLLNFFKVRPDDLKLPNDSQVKKPETYLTAKDGKNNVADITGPIILQTYRAIADYEKGSKTEMTVATGDVVDVVEKSESGWWFCQMKTKRGWVPASYLEPLDSPDEAEDPDPNYAGEPYVTIKAYAAVEEDEVSLSEGEAIEVIHKLLDGWWVVRKGDITGYFPSMYLQKAGEEITQAQRQIRSRGAPPRRSTIRNAQSIHQRSRKRLSQDTYRRNSVRFLQQRRRPARPGPQSPDSKDNPSTPRAKPQPAVPPRPSSDLILHRCTESTKRKLTSAV

Target ORF information:

RefSeq Version	NM_053734.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neutrophil cytosolic factor 1 (Ncf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053734.2

ORF Insert Sequence:

ATGGGGGACA CCTTCATTCG CCACATCGCC CTCCTGGGCT TCGAGAAACG CTTCGTCCCC 
AGCCAACACT ATGTGTACAT GTTCCTGGTT AAGTGGCAGG ACCTGTCGGA GAAGGTGGTC 
TACAGAAAAT TCACCGAGAT CTACGAGTTC CATAAAATGT TAAAGGAGAT GTTCCCCATT 
GAGGCCGGTG AGATCCACAC AGAAAACAGA GTCATCCCTC ACCTCCCAGC TCCCAGGTGG 
TATGATGGGC AGCGTGCAGC GGAGAGCCGC CAGGGAACGC TCACCGAGTA CTTCAACAGC 
CTCATGGGAC TGCCCGTGAA GATCTCCCGC TGCCCACACC TCTTGAACTT CTTCAAAGTG 
CGGCCCGATG ACCTGAAGCT GCCCAATGAC AGCCAGGTGA AGAAGCCAGA GACATACCTG 
ACGGCCAAAG ATGGCAAGAA TAATGTAGCT GACATCACGG GTCCCATCAT CCTTCAGACC 
TATCGGGCCA TCGCTGACTA CGAGAAGGGT TCCAAAACAG AGATGACCGT GGCGACGGGA 
GATGTGGTGG ATGTCGTAGA GAAAAGCGAG AGTGGCTGGT GGTTTTGCCA GATGAAGACA 
AAACGAGGTT GGGTCCCTGC ATCCTATTTG GAGCCCCTTG ACAGCCCTGA TGAGGCAGAG 
GACCCCGATC CCAACTACGC AGGTGAACCG TATGTAACCA TCAAAGCGTA CGCTGCTGTT 
GAAGAGGATG AGGTGTCCCT GTCTGAGGGT GAAGCCATCG AGGTCATTCA TAAGCTCCTA 
GATGGCTGGT GGGTGGTCAG GAAAGGGGAC ATCACCGGCT ACTTCCCATC CATGTATCTG 
CAGAAGGCTG GGGAGGAGAT AACCCAGGCC CAGCGACAGA TTAGAAGCCG CGGGGCACCA 
CCTCGCAGGT CGACCATCCG CAATGCACAG AGCATCCACC AGCGTTCTCG GAAGCGCCTC 
AGCCAGGACA CCTATCGCCG CAACAGCGTC CGATTCCTGC AGCAGCGCAG ACGCCCGGCG 
CGACCTGGGC CGCAGAGCCC TGACTCAAAG GACAATCCAT CGACTCCGCG CGCCAAACCA 
CAGCCTGCGG TGCCTCCGAG ACCCAGCTCG GACCTCATCC TGCACCGCTG CACAGAGAGC 
ACCAAGAGGA AACTGACGTC CGCCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activation of p47phox as a Mechanism of Bupivacaine-Induced Burst Production of Reactive Oxygen Species and Neural Toxicity.
Oxidative medicine and cellular longevity20178539026(2017)
Li YJ,Zhao W,Yu XJ,Li FX,Liu ZT,Li L,Xu SY

Exercise Training Attenuates Upregulation of p47(phox) and p67(phox) in Hearts of Diabetic Rats.
Oxidative medicine and cellular longevity20165868913(2016)
Sharma NM,Rabeler B,Zheng H,Raichlin E,Patel KP

Melatonin nephroprotective action in Zucker diabetic fatty rats involves its inhibitory effect on NADPH oxidase.
Journal of pineal research60(1)109-17(2016 Jan)
Winiarska K,Dzik JM,Labudda M,Focht D,Sierakowski B,Owczarek A,Komorowski L,Bielecki W

Cadmium induces matrix metalloproteinase-9 expression via ROS-dependent EGFR, NF-?B, and AP-1 pathways in human endothelial cells.
Toxicology338104-16(2015 Dec)
Lian S,Xia Y,Khoi PN,Ung TT,Yoon HJ,Kim NH,Kim KK,Jung YD

NADPH oxidase-derived production of reactive oxygen species is involved in learning and memory impairments in 16-month-old female rats.
Molecular medicine reports12(3)4546-53(2015 Sep)
Kan H,Hu W,Wang Y,Wu W,Yin Y,Liang Y,Wang C,Huang D,Li W

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Ncf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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