Hcn3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hcn3
Entrez Gene ID	114245
Full Name	hyperpolarization-activated cyclic nucleotide-gated potassium channel 3
General protein information	Preferred Names hyperpolarization-activated cyclic nucleotide-gated potassium channel 3 Names potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	may play a role in generation of neuronal pacemaker activity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053685.1	NP_446137.1	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HCN3	XP_513856.3
	HCN3	XP_001115891.1
Canis lupus familiaris (dog)	HCN3	XP_547548.2
Mus musculus (house mouse)	Hcn3	NP_032253.1
Homo sapiens (human)	HCN3	NP_065948.1
Rattus norvegicus (Norway rat)	Hcn3	NP_446137.1
Bos taurus (cattle)	HCN3	NP_001179207.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002150	XP_003436040.1

Gene Ontology
Bibliography

Related articles in PubMed

Hyperpolarization-activated cyclic nucleotide-gated channels in peripheral diaphragmatic lymphatics.
Negrini D, Marcozzi C, Solari E, Bossi E, Cinquetti R, Reguzzoni M, Moriondo A
American journal of physiology. Heart and circulatory physiology311(4)H892-H903(2016 Oct)

Expression and cellular localization of HCN channels in rat cerebellar granule neurons.
Z??iga R, Gonz?lez D, Valenzuela C, Brown N, Z??iga L
Biochemical and biophysical research communications478(3)1429-35(2016 09)

Age-associated expression of HCN channel isoforms in rat sinoatrial node.
Huang X, Yang P, Yang Z, Zhang H, Ma A
Experimental biology and medicine (Maywood, N.J.)241(3)331-9(2016 Feb)

Up-regulation of hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) by specific interaction with K+ channel tetramerization domain-containing protein 3 (KCTD3).
Cao-Ehlker X, Zong X, Hammelmann V, Gruner C, Fenske S, Michalakis S, Wahl-Schott C, Biel M
The Journal of biological chemistry288(11)7580-9(2013 Mar)

Investigation of hyperpolarization-activated cyclic nucleotide-gated channels in interstitial cells of Cajal of human bladder.
Xue L, Li Y, Han X, Yao L, Yuan J, Qin W, Liu F, Wang H
Urology80(1)224.e13-8(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that HCN channels might play an important role in affecting contraction frequency of lymphatic muscle cells.
Title: Hyperpolarization-activated cyclic nucleotide-gated channels in peripheral diaphragmatic lymphatics.

the expression and relative abundances of HCN channels in cerebellum and primary cultures of cerebellar granule neurons, is described.
Title: Expression and cellular localization of HCN channels in rat cerebellar granule neurons.

During the senescent process, the HCN2 and HCN4 protein levels declined.
Title: Age-associated expression of HCN channel isoforms in rat sinoatrial node.

The data of this study indicated a HCN subunit shift initiated in the first 24 hours after birth and implicate a prominent perinatal role of the phylogenetically older HCN3 and/or HCN4 subunits in the developing neocortex.
Title: Distinct perinatal features of the hyperpolarization-activated non-selective cation current I(h) in the rat cortical plate.

This study demonistrated that the majority of LHb neurons projecting to the monoaminergic brainstem nuclei express a similar pattern of HCN transcripts, with high levels of HCN2 to HCN4 mRNAs and low levels of HCN1 mRNA.
Title: Lateral habenular neurons projecting to reward-processing monoaminergic nuclei express hyperpolarization-activated cyclic nucleotid-gated cation channels.

The following Hcn3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hcn3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10312	NM_053685.1 Latest version!	Rattus norvegicus hyperpolarization-activated cyclic nucleotide-gated potassium channel 3 (Hcn3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$461.30 $659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hcn3 ( NM_053685.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446137.1 Rattus norvegicus hyperpolarization-activated cyclic nucleotide-gated potassium channel 3 (Hcn3), mRNA.

Price & Availability↑

CloneID	ORa10312
Clone ID Related Accession (Same CDS sequence)	NM_053685.1
Accession Version	NM_053685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF247452.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF247452.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGAGG AGGCGCGGCC GGCGGTGGGG GACGGGGAAG CGGCGACTCC TGCACGCGAG 
ACGCCTCCTG CGGCTCCCGC CCAGGCCCGC GCGGCCTCAG GTGGGGTGCC AGAGTCTGCG 
CCCGAGCCGA AGAGGCGGCA GCTCGGGACG CTGCTGCAGC CGACCGTCAA CAAGTTCTCT 
CTCCGGGTCT TCGGCAGCCA CAAAGCGGTG GAAATCGAGC AGGAGAGGGT GAAGTCCGCC 
GGGGCCTGGA TCATCCACCC CTACAGCGAC TTCCGGTTTT ACTGGGACCT GATCATGCTG 
CTGCTGATGG TGGGGAACCT CATAGTACTG CCCGTGGGCA TCACTTTCTT CAAGGAGGAG 
AACTCCCCAC CCTGGATCGT CTTCAATGTC CTCTCGGACA CTTTCTTCCT GCTGGATCTG 
GTGCTCAACT TCCGAACTGG CATCGTGGTG GAGGAAGGTG CGGAGATCCT GTTGGCGCCC 
AGGGCTATCC GCACGCGTTA CCTGCGCACC TGGTTCCTGG TGGACCTGAT TTCCTCCATC 
CCTGTGGATT ACATCTTCCT AGTGGTAGAG CTGGAGCCAC GACTAGACGC TGAGGTCTAC 
AAAACGGCAC GGGCCCTGCG CATCGTTAGA TTCACCAAGA TCCTTAGCCT GCTGCGGCTG 
CTCCGCCTCT CCCGCCTCAT CCGATACATG CACCAGTGGG AGGAGATCTT TCACATGACC 
TACGACCTGG CCAGTGCAGT GGTTCGCATC TTCAACCTCA TTGGAATGAT GTTGCTGCTG 
TGTCACTGGG ATGGCTGTCT GCAGTTCCTG GTCCCTATGC TGCAGGACTT CCCTTCCGAC 
TGCTGGGTCT CCATGAACCG CATGGTGAAC CACTCGTGGG GCCGCCAGTA CTCCCACGCC 
CTGTTCAAGG CCATGAGTCA CATGCTGTGC ATTGGCTACG GGCAGCAGGC ACCAGTAGGC 
ATGCCTGACG TCTGGCTCAC CATGCTCAGC ATGATTGTGG GCGCCACCTG CTATGCCATG 
TTCATCGGCC ACGCCACCGC CCTCATCCAG TCCCTGGACT CGTCCCGGCG CCAGTACCAG 
GAGAAGTACA AGCAGGTGGA GCAGTACATG TCCTTCCACA AGCTGCCAGC CGACACACGG 
CAGCGCATCC ACGAGTACTA CGAGCATCGG TACCAGGGCA AGATGTTCGA TGAAGAGAGC 
ATCCTGGGGG AGCTGAGCGA GCCGCTTCGG GAGGAGATTA TTAACTTCAC CTGCCGGGGC 
CTGGTGGCCC ACATGCCGCT GTTTGCTCAT GCTGACCCCA GTTTCGTCAC CGCAGTACTC 
ACCAAGCTCC GTTTTGAGGT CTTCCAACCT GGGGATCTGG TGGTGCGTGA GGGCTCCGTG 
GGCAGGAAGA TGTACTTCAT CCAGCATGGG CTGCTCAGTG TGTTGGCACG GGGCGCCCGG 
GACACTCGCC TCACTGACGG ATCCTACTTT GGGGAGATCT GCCTGCTGAC TCGAGGTCGG 
AGAACAGCCA GTGTAAGGGC TGACACCTAC TGTCGCCTCT ACTCACTCAG CGTGGACCAC 
TTCAATGCAG TGCTTGAGGA GTTCCCGATG ATGCGCAGGG CTTTTGAGAC TGTGGCCATG 
GACCGGCTTC GGCGCATCGG CAAAAAGAAT TCGATATTGC AGCGGAAACG CTCTGAGCCG 
AGTCCAGGCA GCAGCAGTGG TGGCGTCATG GAGCAGCATT TGGTACAACA CGACAGAGAC 
ATGGCTCGTG GTATTCGGGG TCTGGCTCCG GGCACAGGAG CTCGCCTCAG TGGAAAGCCA 
GTGCTGTGGG AACCACTGGT ACACGCACCT CTGCAGGCAG CTGCTGTGAC CTCCAACGTG 
GCCATAGCCT TGACTCATCA GCGAGGCCCT CTGCCCCTCT CCCCTGATTC TCCAGCCACC 
CTCCTGGCTC GATCTGCTAG ACGCTCAGCA GGCTCCCCAG CCTCCCCACT GGTGCCTGTC 
CGAGCAGGTC CTCTGCTGGC CCGGGGACCC TGGGCGTCCA CTTCTCGCCT GCCTGCTCCA 
CCTGCCCGAA CCCTCCATGC CAGCCTATCC CGGACAGGGC GTTCCCAGGT GTCTCTGTTG 
GGCCCTCCCC CAGGAGGAGG TGGTCGGAGG CTAGGACCTC GGGGCCGCCC ACTCTCTGCC 
TCTCAACCCT CTCTGCCTCA GCGAGCCACG GGGGATGGCT CTCCTAGGCG CAAAGGCTCT 
GGAAGTGAGC GTCTGCCCCC CTCGGGGCTC CTGGCCAAGC CTCCAGGGAC TGTCCAGCCA 
TCCAGGTCAT CAGTGCCTGA GCCAGTTACC CCCAGAGGTC CCCAAATTTC TGCCAACATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446137.1
CDS	1..2343
Translation	MEEEARPAVGDGEAATPARETPPAAPAQARAASGGVPESAPEPKRRQLGTLLQPTVNKFSLRVFGSHKAVEIEQERVKSAGAWIIHPYSDFRFYWDLIMLLLMVGNLIVLPVGITFFKEENSPPWIVFNVLSDTFFLLDLVLNFRTGIVVEEGAEILLAPRAIRTRYLRTWFLVDLISSIPVDYIFLVVELEPRLDAEVYKTARALRIVRFTKILSLLRLLRLSRLIRYMHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPMLQDFPSDCWVSMNRMVNHSWGRQYSHALFKAMSHMLCIGYGQQAPVGMPDVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADTRQRIHEYYEHRYQGKMFDEESILGELSEPLREEIINFTCRGLVAHMPLFAHADPSFVTAVLTKLRFEVFQPGDLVVREGSVGRKMYFIQHGLLSVLARGARDTRLTDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDHFNAVLEEFPMMRRAFETVAMDRLRRIGKKNSILQRKRSEPSPGSSSGGVMEQHLVQHDRDMARGIRGLAPGTGARLSGKPVLWEPLVHAPLQAAAVTSNVAIALTHQRGPLPLSPDSPATLLARSARRSAGSPASPLVPVRAGPLLARGPWASTSRLPAPPARTLHASLSRTGRSQVSLLGPPPGGGGRRLGPRGRPLSASQPSLPQRATGDGSPRRKGSGSERLPPSGLLAKPPGTVQPSRSSVPEPVTPRGPQISANM

Target ORF information:

RefSeq Version	NM_053685.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hyperpolarization-activated cyclic nucleotide-gated potassium channel 3 (Hcn3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053685.1

ORF Insert Sequence:

ATGGAGGAGG AGGCGCGGCC GGCGGTGGGG GACGGGGAAG CGGCGACTCC TGCACGCGAG 
ACGCCTCCTG CGGCTCCCGC CCAGGCCCGC GCGGCCTCAG GTGGGGTGCC AGAGTCTGCG 
CCCGAGCCGA AGAGGCGGCA GCTCGGGACG CTGCTGCAGC CGACCGTCAA CAAGTTCTCT 
CTCCGGGTCT TCGGCAGCCA CAAAGCGGTG GAAATCGAGC AGGAGAGGGT GAAGTCCGCC 
GGGGCCTGGA TCATCCACCC CTACAGCGAC TTCCGGTTTT ACTGGGACCT GATCATGCTG 
CTGCTGATGG TGGGGAACCT CATAGTACTG CCCGTGGGCA TCACTTTCTT CAAGGAGGAG 
AACTCCCCAC CCTGGATCGT CTTCAATGTC CTCTCGGACA CTTTCTTCCT GCTGGATCTG 
GTGCTCAACT TCCGAACTGG CATCGTGGTG GAGGAAGGTG CGGAGATCCT GTTGGCGCCC 
AGGGCTATCC GCACGCGTTA CCTGCGCACC TGGTTCCTGG TGGACCTGAT TTCCTCCATC 
CCTGTGGATT ACATCTTCCT AGTGGTAGAG CTGGAGCCAC GACTAGACGC TGAGGTCTAC 
AAAACGGCAC GGGCCCTGCG CATCGTTAGA TTCACCAAGA TCCTTAGCCT GCTGCGGCTG 
CTCCGCCTCT CCCGCCTCAT CCGATACATG CACCAGTGGG AGGAGATCTT TCACATGACC 
TACGACCTGG CCAGTGCAGT GGTTCGCATC TTCAACCTCA TTGGAATGAT GTTGCTGCTG 
TGTCACTGGG ATGGCTGTCT GCAGTTCCTG GTCCCTATGC TGCAGGACTT CCCTTCCGAC 
TGCTGGGTCT CCATGAACCG CATGGTGAAC CACTCGTGGG GCCGCCAGTA CTCCCACGCC 
CTGTTCAAGG CCATGAGTCA CATGCTGTGC ATTGGCTACG GGCAGCAGGC ACCAGTAGGC 
ATGCCTGACG TCTGGCTCAC CATGCTCAGC ATGATTGTGG GCGCCACCTG CTATGCCATG 
TTCATCGGCC ACGCCACCGC CCTCATCCAG TCCCTGGACT CGTCCCGGCG CCAGTACCAG 
GAGAAGTACA AGCAGGTGGA GCAGTACATG TCCTTCCACA AGCTGCCAGC CGACACACGG 
CAGCGCATCC ACGAGTACTA CGAGCATCGG TACCAGGGCA AGATGTTCGA TGAAGAGAGC 
ATCCTGGGGG AGCTGAGCGA GCCGCTTCGG GAGGAGATTA TTAACTTCAC CTGCCGGGGC 
CTGGTGGCCC ACATGCCGCT GTTTGCTCAT GCTGACCCCA GTTTCGTCAC CGCAGTACTC 
ACCAAGCTCC GTTTTGAGGT CTTCCAACCT GGGGATCTGG TGGTGCGTGA GGGCTCCGTG 
GGCAGGAAGA TGTACTTCAT CCAGCATGGG CTGCTCAGTG TGTTGGCACG GGGCGCCCGG 
GACACTCGCC TCACTGACGG ATCCTACTTT GGGGAGATCT GCCTGCTGAC TCGAGGTCGG 
AGAACAGCCA GTGTAAGGGC TGACACCTAC TGTCGCCTCT ACTCACTCAG CGTGGACCAC 
TTCAATGCAG TGCTTGAGGA GTTCCCGATG ATGCGCAGGG CTTTTGAGAC TGTGGCCATG 
GACCGGCTTC GGCGCATCGG CAAAAAGAAT TCGATATTGC AGCGGAAACG CTCTGAGCCG 
AGTCCAGGCA GCAGCAGTGG TGGCGTCATG GAGCAGCATT TGGTACAACA CGACAGAGAC 
ATGGCTCGTG GTATTCGGGG TCTGGCTCCG GGCACAGGAG CTCGCCTCAG TGGAAAGCCA 
GTGCTGTGGG AACCACTGGT ACACGCACCT CTGCAGGCAG CTGCTGTGAC CTCCAACGTG 
GCCATAGCCT TGACTCATCA GCGAGGCCCT CTGCCCCTCT CCCCTGATTC TCCAGCCACC 
CTCCTGGCTC GATCTGCTAG ACGCTCAGCA GGCTCCCCAG CCTCCCCACT GGTGCCTGTC 
CGAGCAGGTC CTCTGCTGGC CCGGGGACCC TGGGCGTCCA CTTCTCGCCT GCCTGCTCCA 
CCTGCCCGAA CCCTCCATGC CAGCCTATCC CGGACAGGGC GTTCCCAGGT GTCTCTGTTG 
GGCCCTCCCC CAGGAGGAGG TGGTCGGAGG CTAGGACCTC GGGGCCGCCC ACTCTCTGCC 
TCTCAACCCT CTCTGCCTCA GCGAGCCACG GGGGATGGCT CTCCTAGGCG CAAAGGCTCT 
GGAAGTGAGC GTCTGCCCCC CTCGGGGCTC CTGGCCAAGC CTCCAGGGAC TGTCCAGCCA 
TCCAGGTCAT CAGTGCCTGA GCCAGTTACC CCCAGAGGTC CCCAAATTTC TGCCAACATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hyperpolarization-activated cyclic nucleotide-gated channels in peripheral diaphragmatic lymphatics.
American journal of physiology. Heart and circulatory physiology311(4)H892-H903(2016 Oct)
Negrini D,Marcozzi C,Solari E,Bossi E,Cinquetti R,Reguzzoni M,Moriondo A

Expression and cellular localization of HCN channels in rat cerebellar granule neurons.
Biochemical and biophysical research communications478(3)1429-35(2016 09)
Z??iga R,Gonz?lez D,Valenzuela C,Brown N,Z??iga L

Age-associated expression of HCN channel isoforms in rat sinoatrial node.
Experimental biology and medicine (Maywood, N.J.)241(3)331-9(2016 Feb)
Huang X,Yang P,Yang Z,Zhang H,Ma A

Up-regulation of hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) by specific interaction with K+ channel tetramerization domain-containing protein 3 (KCTD3).
The Journal of biological chemistry288(11)7580-9(2013 Mar)
Cao-Ehlker X,Zong X,Hammelmann V,Gruner C,Fenske S,Michalakis S,Wahl-Schott C,Biel M

Investigation of hyperpolarization-activated cyclic nucleotide-gated channels in interstitial cells of Cajal of human bladder.
Urology80(1)224.e13-8(2012 Jul)
Xue L,Li Y,Han X,Yao L,Yuan J,Qin W,Liu F,Wang H

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Hcn3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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