Tmf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmf1
Entrez Gene ID	114206
Full Name	TATA element modulatory factor 1
General protein information	Preferred Names TATA element modulatory factor 1 Names TATA element modulatory factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34
Summary	human homolog interacts with the androgen receptor and may act as a coactivator [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008763143.2	XP_008761365.1	TATA element modulatory factor isoform X1
XM_017592378.1	XP_017447867.1	TATA element modulatory factor isoform X1
NM_053671.2	NP_446123.2	TATA element modulatory factor

Pathways from BioSystems

Homologies

	TMF1	XP_001088711.2
Danio rerio (zebrafish)	tmf1	NP_001003522.1
Canis lupus familiaris (dog)	TMF1	XP_005632331.1
Homo sapiens (human)	TMF1	NP_009045.2
Bos taurus (cattle)	TMF1	NP_001193189.1
Pan troglodytes (chimpanzee)	TMF1	XP_526227.2
Mus musculus (house mouse)	Tmf1	NP_001074580.1
Rattus norvegicus (Norway rat)	Tmf1	NP_446123.2
Gallus gallus (chicken)	TMF1	XP_423749.2

Gene Ontology
Bibliography

Related articles in PubMed

Testosterone deficiency accompanied by testicular and epididymal abnormalities in TMF(-/-) mice.
Elkis Y, Bel S, Lerer-Goldstein T, Nyska A, Creasy DM, Shpungin S, Nir U
Molecular and cellular endocrinology365(1)52-63(2013 Jan)

Loss of TMF/ARA160 protein renders colonic mucus refractory to bacterial colonization and diminishes intestinal susceptibility to acute colitis.
Bel S, Elkis Y, Lerer-Goldstein T, Nyska A, Shpungin S, Nir U
The Journal of biological chemistry287(30)25631-9(2012 Jul)

CTC1 deletion results in defective telomere replication, leading to catastrophic telomere loss and stem cell exhaustion.
Gu P, Min JN, Wang Y, Huang C, Peng T, Chai W, Chang S
The EMBO journal31(10)2309-21(2012 May)

TMF/ARA160: A key regulator of sperm development.
Lerer-Goldshtein T, Bel S, Shpungin S, Pery E, Motro B, Goldstein RS, Bar-Sheshet SI, Breitbart H, Nir U
Developmental biology348(1)12-21(2010 Dec)

trnp: A conserved mammalian gene encoding a nuclear protein that accelerates cell-cycle progression.
Volpe M, Shpungin S, Barbi C, Abrham G, Malovani H, Wides R, Nir U
DNA and cell biology25(6)331-9(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tmf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa43922	XM_017592378.1 Latest version!	Rattus norvegicus TATA element modulatory factor 1 (Tmf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa43922	XM_008763143.2 Latest version!	Rattus norvegicus TATA element modulatory factor 1 (Tmf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa10275	NM_053671.2 Latest version!	Rattus norvegicus TATA element modulatory factor 1 (Tmf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmf1 ( XM_008763143.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761365.1 Rattus norvegicus TATA element modulatory factor 1 (Tmf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43922
Clone ID Related Accession (Same CDS sequence)	XM_008763143.2 , XM_017592378.1
Accession Version	XM_008763143.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	TATA element modulatory factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGT TCAATGCCTC GCAGTTGTCC AGCTTCGCCA AGCAGGCCCT GTCCCAGGCC 
CAGAAGTCCA TCGACAGGGT CCTGGACATC CAGGAGGAAG AGCCGAGCGC CTGGGCCGAG 
GCCATCCCGT ACGGTGAGCC AGGAATAAGT CCCTCTGTGA GTGGAGGATG GGATACTTCA 
ACCTGGGGAT TGAACTCAAG CTCTGAACCT CAGAGTTCAC CAACAGCCTC TCAAGCAATT 
ACAAAGCCAG TTCGGAGGAC TGTAGTAGAT GAATCTGAAA ATTTCTTCAG TGCCTTTCTC 
TCTCCATCGG ATGTGCAGAC CATTCAGAAG AGTCCAGTGG TCTCAAAGCC TCCTGCTAAA 
TCACAGCGAC CAGAAGAAGA AGTGAAAAGC AGTTTACAAG AACCATCGTC TCCTGGCCAG 
TCCAGAACCT CTGAGACAGC GGAGTCTGAA GCGACAGACT CTGCCTGTGT TTCGGGAGAG 
ACTCCAGCCG TGGGCACTCC CTCACCTGTC CCTGAAGGCA AGCGTGAAGA AACTGCTAGT 
GAAGAGTCTG AGGGGAAGGT GCCCACCGTG AGAGCGAAGG CGTCTGAAAG CGTAGTTAAG 
GTAAACACAG CAGAAGATGT ATCTCCCACA TCCACTCAGT CTCTTACGAC CGAAACGAAG 
GATATGGCTT TGGAGCCTAA AGAACAAAAA CACGAAGACA GACAAAGCAA CACACCTTCT 
CCTCCTGTTA GCTCCTTCTC GTCAGGCACT TCCACCACCA GTGACATTGA AGTTTTAGAT 
CATGAAAGTG TAATAAGTGA GAGCTCAGCT AGTTCAAGGC AAGAGACTTC AGATGCAAAG 
TCAAGTCTTC ACCTCATGCA GACCTCATTT CAGCTTCTCT CTGCATCTGC TTGTCCTGAG 
TATGGCCGCT TAGATGACTT CCAAAAGCTC AGTGAGAGTT GCTGCTCTTC GGATGCTTTT 
GAGAGAATAG ACTCGTTTAG CGTGCAGTCT CTGGATAGCC GGAGTGTGAG TGAAATCAAC 
TCGGACGACG AACTGCCGGG TAGAGGCTAT GCTTTAGTAC CTATAGTCGT GAGTCCTTCA 
GCCCCGAAGA CAAAAGTAGT AGAATCTACT GAGGAAAACG TTGAAGAAGA GGAAGGAAAC 
GAGACATTAA TTGCACCTTC TGAGGAAGCA GAATTAGAGG AAAGTGGCCG AAGTGCTACT 
CCAGTTAACT GTGACCAGCC AGATACATTG GCTTCTCCTA CACCAGTAAA TGTTGGGCCC 
TCTGCCTCTG GACCGGCCGC TGAGGATGGT GAAGCTGCTG AAAACCACCC AAAAGCACCA 
CAGCTGGAGC AAGAAGATGT TGGCAAGACA GTTGAATTTC TGAATGAGAA GCTAGAAAAG 
AGGGAGACTC AATTATTGTC ACTTAGTAAG GAAAAGGCAC TTCTAGAGGA AGCTTATGAT 
AACCTGAAGG ATGAAATGTT CAGAGTAAAA GAAGAAAGTA GTAGCATTTC TTCCCTGAAA 
GATGAGTTTA CCCAGAGGAT CGCAGAAGCA GAAAGGAAAG CTCAACTCGC CTGCAAAGAG 
AGAGACACTG CGAAGAAGGA AATGAAAACC ATGAAGGAAG AACTTGCCTC TAGATTAAAT 
AGTAGTCAGA CTGCAGACCT TCTGAAGGAA AAGGATGAAC AGATTCAAGG CTTAATGGAA 
GAAGGAGAAA AACTTTCGAA GCAGCAGCTG CATAATTCCA ACATGATTAA GAAATTAAGA 
GCTAAGGATA AAGACAATGA AAGTGTTATT GCAAAGCTGA AGAAAAAAGC TAAGGAGCTG 
GAAGAGGAGC TACAGCATTT AAGACAGGTC CTTGATGGCA AAGAAGAGGT TGAGAAACAA 
CATAGAGAAA ACATTAAAAA ACTAAATTCT GTAGTAGAAC GCCAGGAAAA AGATCTTGGC 
CGACTTCAGG TAGACATGAA TGAACTTGAA GAAAAGAGCC GAAGTACTCA GGCTGCCCTA 
GACAGTGCCT ACAGAGAACT TACTGATCTT CACAAAGCCA ACGCTGCTAA GGACAGTGAG 
GTACAAGAAG CTGCTCTGAG GCGTGAGATG AAAGCTAAAG AAGAACTTTC TGGGGCACTG 
GAGAAGGCCC AGGAAGAAGC CCGTCAGCAG CAAGAGACTT TAGCACTTCA GGTTGGGGAC 
CTTAGGCTAG CGCTGCAACG GGCAGAGCAA GCAGCTGCCA GGAAAGAGGA TTACTTACGC 
CATGAGATCA GTGAACTTCA GCAGAGACTC CAAGAAGCAG AGAATCGAAA CCAGGAGCTG 
AGTCAAAGTG TTTCATCAAC AACAAGACCT TTGCTTCGAC AGATAGAAAA TTTACAAGCA 
ACTCTAGGAT CCCAGACATC GTCATGGGAG ATGCTAGAGA AGAATCTTTC TGATCGGCTC 
GGTGAATCAC AGACCTTGTT GGCTGCAGCA GTTGAAAGAG AACGTGCAGC TACGGAAGAA 
CTCCTAGCCA ACAAAATCCA GATGTCTTCA GTGGAGTCGC AGAATGCATT ACTACGACAG 
GAAAACAGTA GACTGCAGGC CCAGCTAGAG TCAGAGAAAA ATAAATTAAG AAAACTAGAG 
GATGAAAATA GCCGGTACCA GGTTGAATTA GAAAATCTAA AAGATGAATA TGTAAGAACA 
CTTGAAGAGG CAAGGAAAGA AAAGACACTT TTGAGCAGTC AGTTAGAAAT GGAAAGAATG 
AAAGTTGAAC AAGAAAGGAA GAAAACCATT TTCACTCAAG AAACACTAAA AGAAAAGTCT 
TACAGAGTTT TTTTCCCCCT AAGGAGCACA AGCCGTTTTC CGTTTCTAGT ACTCCCACCA 
TGTCACGATC CAGTTCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761365.1
CDS	1596..4436
Translation	MSWFNASQLSSFAKQALSQAQKSIDRVLDIQEEEPSAWAEAIPYGEPGISPSVSGGWDTSTWGLNSSSEPQSSPTASQAITKPVRRTVVDESENFFSAFLSPSDVQTIQKSPVVSKPPAKSQRPEEEVKSSLQEPSSPGQSRTSETAESEATDSACVSGETPAVGTPSPVPEGKREETASEESEGKVPTVRAKASESVVKVNTAEDVSPTSTQSLTTETKDMALEPKEQKHEDRQSNTPSPPVSSFSSGTSTTSDIEVLDHESVISESSASSRQETSDAKSSLHLMQTSFQLLSASACPEYGRLDDFQKLSESCCSSDAFERIDSFSVQSLDSRSVSEINSDDELPGRGYALVPIVVSPSAPKTKVVESTEENVEEEEGNETLIAPSEEAELEESGRSATPVNCDQPDTLASPTPVNVGPSASGPAAEDGEAAENHPKAPQLEQEDVGKTVEFLNEKLEKRETQLLSLSKEKALLEEAYDNLKDEMFRVKEESSSISSLKDEFTQRIAEAERKAQLACKERDTAKKEMKTMKEELASRLNSSQTADLLKEKDEQIQGLMEEGEKLSKQQLHNSNMIKKLRAKDKDNESVIAKLKKKAKELEEELQHLRQVLDGKEEVEKQHRENIKKLNSVVERQEKDLGRLQVDMNELEEKSRSTQAALDSAYRELTDLHKANAAKDSEVQEAALRREMKAKEELSGALEKAQEEARQQQETLALQVGDLRLALQRAEQAAARKEDYLRHEISELQQRLQEAENRNQELSQSVSSTTRPLLRQIENLQATLGSQTSSWEMLEKNLSDRLGESQTLLAAAVERERAATEELLANKIQMSSVESQNALLRQENSRLQAQLESEKNKLRKLEDENSRYQVELENLKDEYVRTLEEARKEKTLLSSQLEMERMKVEQERKKTIFTQETLKEKSYRVFFPLRSTSRFPFLVLPPCHDPVL

Target ORF information:

RefSeq Version	XM_008763143.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TATA element modulatory factor 1 (Tmf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763143.2

ORF Insert Sequence:

ATGAGCTGGT TCAATGCCTC GCAGTTGTCC AGCTTCGCCA AGCAGGCCCT GTCCCAGGCC 
CAGAAGTCCA TCGACAGGGT CCTGGACATC CAGGAGGAAG AGCCGAGCGC CTGGGCCGAG 
GCCATCCCGT ACGGTGAGCC AGGAATAAGT CCCTCTGTGA GTGGAGGATG GGATACTTCA 
ACCTGGGGAT TGAACTCAAG CTCTGAACCT CAGAGTTCAC CAACAGCCTC TCAAGCAATT 
ACAAAGCCAG TTCGGAGGAC TGTAGTAGAT GAATCTGAAA ATTTCTTCAG TGCCTTTCTC 
TCTCCATCGG ATGTGCAGAC CATTCAGAAG AGTCCAGTGG TCTCAAAGCC TCCTGCTAAA 
TCACAGCGAC CAGAAGAAGA AGTGAAAAGC AGTTTACAAG AACCATCGTC TCCTGGCCAG 
TCCAGAACCT CTGAGACAGC GGAGTCTGAA GCGACAGACT CTGCCTGTGT TTCGGGAGAG 
ACTCCAGCCG TGGGCACTCC CTCACCTGTC CCTGAAGGCA AGCGTGAAGA AACTGCTAGT 
GAAGAGTCTG AGGGGAAGGT GCCCACCGTG AGAGCGAAGG CGTCTGAAAG CGTAGTTAAG 
GTAAACACAG CAGAAGATGT ATCTCCCACA TCCACTCAGT CTCTTACGAC CGAAACGAAG 
GATATGGCTT TGGAGCCTAA AGAACAAAAA CACGAAGACA GACAAAGCAA CACACCTTCT 
CCTCCTGTTA GCTCCTTCTC GTCAGGCACT TCCACCACCA GTGACATTGA AGTTTTAGAT 
CATGAAAGTG TAATAAGTGA GAGCTCAGCT AGTTCAAGGC AAGAGACTTC AGATGCAAAG 
TCAAGTCTTC ACCTCATGCA GACCTCATTT CAGCTTCTCT CTGCATCTGC TTGTCCTGAG 
TATGGCCGCT TAGATGACTT CCAAAAGCTC AGTGAGAGTT GCTGCTCTTC GGATGCTTTT 
GAGAGAATAG ACTCGTTTAG CGTGCAGTCT CTGGATAGCC GGAGTGTGAG TGAAATCAAC 
TCGGACGACG AACTGCCGGG TAGAGGCTAT GCTTTAGTAC CTATAGTCGT GAGTCCTTCA 
GCCCCGAAGA CAAAAGTAGT AGAATCTACT GAGGAAAACG TTGAAGAAGA GGAAGGAAAC 
GAGACATTAA TTGCACCTTC TGAGGAAGCA GAATTAGAGG AAAGTGGCCG AAGTGCTACT 
CCAGTTAACT GTGACCAGCC AGATACATTG GCTTCTCCTA CACCAGTAAA TGTTGGGCCC 
TCTGCCTCTG GACCGGCCGC TGAGGATGGT GAAGCTGCTG AAAACCACCC AAAAGCACCA 
CAGCTGGAGC AAGAAGATGT TGGCAAGACA GTTGAATTTC TGAATGAGAA GCTAGAAAAG 
AGGGAGACTC AATTATTGTC ACTTAGTAAG GAAAAGGCAC TTCTAGAGGA AGCTTATGAT 
AACCTGAAGG ATGAAATGTT CAGAGTAAAA GAAGAAAGTA GTAGCATTTC TTCCCTGAAA 
GATGAGTTTA CCCAGAGGAT CGCAGAAGCA GAAAGGAAAG CTCAACTCGC CTGCAAAGAG 
AGAGACACTG CGAAGAAGGA AATGAAAACC ATGAAGGAAG AACTTGCCTC TAGATTAAAT 
AGTAGTCAGA CTGCAGACCT TCTGAAGGAA AAGGATGAAC AGATTCAAGG CTTAATGGAA 
GAAGGAGAAA AACTTTCGAA GCAGCAGCTG CATAATTCCA ACATGATTAA GAAATTAAGA 
GCTAAGGATA AAGACAATGA AAGTGTTATT GCAAAGCTGA AGAAAAAAGC TAAGGAGCTG 
GAAGAGGAGC TACAGCATTT AAGACAGGTC CTTGATGGCA AAGAAGAGGT TGAGAAACAA 
CATAGAGAAA ACATTAAAAA ACTAAATTCT GTAGTAGAAC GCCAGGAAAA AGATCTTGGC 
CGACTTCAGG TAGACATGAA TGAACTTGAA GAAAAGAGCC GAAGTACTCA GGCTGCCCTA 
GACAGTGCCT ACAGAGAACT TACTGATCTT CACAAAGCCA ACGCTGCTAA GGACAGTGAG 
GTACAAGAAG CTGCTCTGAG GCGTGAGATG AAAGCTAAAG AAGAACTTTC TGGGGCACTG 
GAGAAGGCCC AGGAAGAAGC CCGTCAGCAG CAAGAGACTT TAGCACTTCA GGTTGGGGAC 
CTTAGGCTAG CGCTGCAACG GGCAGAGCAA GCAGCTGCCA GGAAAGAGGA TTACTTACGC 
CATGAGATCA GTGAACTTCA GCAGAGACTC CAAGAAGCAG AGAATCGAAA CCAGGAGCTG 
AGTCAAAGTG TTTCATCAAC AACAAGACCT TTGCTTCGAC AGATAGAAAA TTTACAAGCA 
ACTCTAGGAT CCCAGACATC GTCATGGGAG ATGCTAGAGA AGAATCTTTC TGATCGGCTC 
GGTGAATCAC AGACCTTGTT GGCTGCAGCA GTTGAAAGAG AACGTGCAGC TACGGAAGAA 
CTCCTAGCCA ACAAAATCCA GATGTCTTCA GTGGAGTCGC AGAATGCATT ACTACGACAG 
GAAAACAGTA GACTGCAGGC CCAGCTAGAG TCAGAGAAAA ATAAATTAAG AAAACTAGAG 
GATGAAAATA GCCGGTACCA GGTTGAATTA GAAAATCTAA AAGATGAATA TGTAAGAACA 
CTTGAAGAGG CAAGGAAAGA AAAGACACTT TTGAGCAGTC AGTTAGAAAT GGAAAGAATG 
AAAGTTGAAC AAGAAAGGAA GAAAACCATT TTCACTCAAG AAACACTAAA AGAAAAGTCT 
TACAGAGTTT TTTTCCCCCT AAGGAGCACA AGCCGTTTTC CGTTTCTAGT ACTCCCACCA 
TGTCACGATC CAGTTCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmf1 ( XM_017592378.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447867.1 Rattus norvegicus TATA element modulatory factor 1 (Tmf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa43922
Clone ID Related Accession (Same CDS sequence)	XM_008763143.2 , XM_017592378.1
Accession Version	XM_017592378.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	TATA element modulatory factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGT TCAATGCCTC GCAGTTGTCC AGCTTCGCCA AGCAGGCCCT GTCCCAGGCC 
CAGAAGTCCA TCGACAGGGT CCTGGACATC CAGGAGGAAG AGCCGAGCGC CTGGGCCGAG 
GCCATCCCGT ACGGTGAGCC AGGAATAAGT CCCTCTGTGA GTGGAGGATG GGATACTTCA 
ACCTGGGGAT TGAACTCAAG CTCTGAACCT CAGAGTTCAC CAACAGCCTC TCAAGCAATT 
ACAAAGCCAG TTCGGAGGAC TGTAGTAGAT GAATCTGAAA ATTTCTTCAG TGCCTTTCTC 
TCTCCATCGG ATGTGCAGAC CATTCAGAAG AGTCCAGTGG TCTCAAAGCC TCCTGCTAAA 
TCACAGCGAC CAGAAGAAGA AGTGAAAAGC AGTTTACAAG AACCATCGTC TCCTGGCCAG 
TCCAGAACCT CTGAGACAGC GGAGTCTGAA GCGACAGACT CTGCCTGTGT TTCGGGAGAG 
ACTCCAGCCG TGGGCACTCC CTCACCTGTC CCTGAAGGCA AGCGTGAAGA AACTGCTAGT 
GAAGAGTCTG AGGGGAAGGT GCCCACCGTG AGAGCGAAGG CGTCTGAAAG CGTAGTTAAG 
GTAAACACAG CAGAAGATGT ATCTCCCACA TCCACTCAGT CTCTTACGAC CGAAACGAAG 
GATATGGCTT TGGAGCCTAA AGAACAAAAA CACGAAGACA GACAAAGCAA CACACCTTCT 
CCTCCTGTTA GCTCCTTCTC GTCAGGCACT TCCACCACCA GTGACATTGA AGTTTTAGAT 
CATGAAAGTG TAATAAGTGA GAGCTCAGCT AGTTCAAGGC AAGAGACTTC AGATGCAAAG 
TCAAGTCTTC ACCTCATGCA GACCTCATTT CAGCTTCTCT CTGCATCTGC TTGTCCTGAG 
TATGGCCGCT TAGATGACTT CCAAAAGCTC AGTGAGAGTT GCTGCTCTTC GGATGCTTTT 
GAGAGAATAG ACTCGTTTAG CGTGCAGTCT CTGGATAGCC GGAGTGTGAG TGAAATCAAC 
TCGGACGACG AACTGCCGGG TAGAGGCTAT GCTTTAGTAC CTATAGTCGT GAGTCCTTCA 
GCCCCGAAGA CAAAAGTAGT AGAATCTACT GAGGAAAACG TTGAAGAAGA GGAAGGAAAC 
GAGACATTAA TTGCACCTTC TGAGGAAGCA GAATTAGAGG AAAGTGGCCG AAGTGCTACT 
CCAGTTAACT GTGACCAGCC AGATACATTG GCTTCTCCTA CACCAGTAAA TGTTGGGCCC 
TCTGCCTCTG GACCGGCCGC TGAGGATGGT GAAGCTGCTG AAAACCACCC AAAAGCACCA 
CAGCTGGAGC AAGAAGATGT TGGCAAGACA GTTGAATTTC TGAATGAGAA GCTAGAAAAG 
AGGGAGACTC AATTATTGTC ACTTAGTAAG GAAAAGGCAC TTCTAGAGGA AGCTTATGAT 
AACCTGAAGG ATGAAATGTT CAGAGTAAAA GAAGAAAGTA GTAGCATTTC TTCCCTGAAA 
GATGAGTTTA CCCAGAGGAT CGCAGAAGCA GAAAGGAAAG CTCAACTCGC CTGCAAAGAG 
AGAGACACTG CGAAGAAGGA AATGAAAACC ATGAAGGAAG AACTTGCCTC TAGATTAAAT 
AGTAGTCAGA CTGCAGACCT TCTGAAGGAA AAGGATGAAC AGATTCAAGG CTTAATGGAA 
GAAGGAGAAA AACTTTCGAA GCAGCAGCTG CATAATTCCA ACATGATTAA GAAATTAAGA 
GCTAAGGATA AAGACAATGA AAGTGTTATT GCAAAGCTGA AGAAAAAAGC TAAGGAGCTG 
GAAGAGGAGC TACAGCATTT AAGACAGGTC CTTGATGGCA AAGAAGAGGT TGAGAAACAA 
CATAGAGAAA ACATTAAAAA ACTAAATTCT GTAGTAGAAC GCCAGGAAAA AGATCTTGGC 
CGACTTCAGG TAGACATGAA TGAACTTGAA GAAAAGAGCC GAAGTACTCA GGCTGCCCTA 
GACAGTGCCT ACAGAGAACT TACTGATCTT CACAAAGCCA ACGCTGCTAA GGACAGTGAG 
GTACAAGAAG CTGCTCTGAG GCGTGAGATG AAAGCTAAAG AAGAACTTTC TGGGGCACTG 
GAGAAGGCCC AGGAAGAAGC CCGTCAGCAG CAAGAGACTT TAGCACTTCA GGTTGGGGAC 
CTTAGGCTAG CGCTGCAACG GGCAGAGCAA GCAGCTGCCA GGAAAGAGGA TTACTTACGC 
CATGAGATCA GTGAACTTCA GCAGAGACTC CAAGAAGCAG AGAATCGAAA CCAGGAGCTG 
AGTCAAAGTG TTTCATCAAC AACAAGACCT TTGCTTCGAC AGATAGAAAA TTTACAAGCA 
ACTCTAGGAT CCCAGACATC GTCATGGGAG ATGCTAGAGA AGAATCTTTC TGATCGGCTC 
GGTGAATCAC AGACCTTGTT GGCTGCAGCA GTTGAAAGAG AACGTGCAGC TACGGAAGAA 
CTCCTAGCCA ACAAAATCCA GATGTCTTCA GTGGAGTCGC AGAATGCATT ACTACGACAG 
GAAAACAGTA GACTGCAGGC CCAGCTAGAG TCAGAGAAAA ATAAATTAAG AAAACTAGAG 
GATGAAAATA GCCGGTACCA GGTTGAATTA GAAAATCTAA AAGATGAATA TGTAAGAACA 
CTTGAAGAGG CAAGGAAAGA AAAGACACTT TTGAGCAGTC AGTTAGAAAT GGAAAGAATG 
AAAGTTGAAC AAGAAAGGAA GAAAACCATT TTCACTCAAG AAACACTAAA AGAAAAGTCT 
TACAGAGTTT TTTTCCCCCT AAGGAGCACA AGCCGTTTTC CGTTTCTAGT ACTCCCACCA 
TGTCACGATC CAGTTCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447867.1
CDS	1596..4436
Translation	MSWFNASQLSSFAKQALSQAQKSIDRVLDIQEEEPSAWAEAIPYGEPGISPSVSGGWDTSTWGLNSSSEPQSSPTASQAITKPVRRTVVDESENFFSAFLSPSDVQTIQKSPVVSKPPAKSQRPEEEVKSSLQEPSSPGQSRTSETAESEATDSACVSGETPAVGTPSPVPEGKREETASEESEGKVPTVRAKASESVVKVNTAEDVSPTSTQSLTTETKDMALEPKEQKHEDRQSNTPSPPVSSFSSGTSTTSDIEVLDHESVISESSASSRQETSDAKSSLHLMQTSFQLLSASACPEYGRLDDFQKLSESCCSSDAFERIDSFSVQSLDSRSVSEINSDDELPGRGYALVPIVVSPSAPKTKVVESTEENVEEEEGNETLIAPSEEAELEESGRSATPVNCDQPDTLASPTPVNVGPSASGPAAEDGEAAENHPKAPQLEQEDVGKTVEFLNEKLEKRETQLLSLSKEKALLEEAYDNLKDEMFRVKEESSSISSLKDEFTQRIAEAERKAQLACKERDTAKKEMKTMKEELASRLNSSQTADLLKEKDEQIQGLMEEGEKLSKQQLHNSNMIKKLRAKDKDNESVIAKLKKKAKELEEELQHLRQVLDGKEEVEKQHRENIKKLNSVVERQEKDLGRLQVDMNELEEKSRSTQAALDSAYRELTDLHKANAAKDSEVQEAALRREMKAKEELSGALEKAQEEARQQQETLALQVGDLRLALQRAEQAAARKEDYLRHEISELQQRLQEAENRNQELSQSVSSTTRPLLRQIENLQATLGSQTSSWEMLEKNLSDRLGESQTLLAAAVERERAATEELLANKIQMSSVESQNALLRQENSRLQAQLESEKNKLRKLEDENSRYQVELENLKDEYVRTLEEARKEKTLLSSQLEMERMKVEQERKKTIFTQETLKEKSYRVFFPLRSTSRFPFLVLPPCHDPVL

Target ORF information:

RefSeq Version	XM_017592378.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TATA element modulatory factor 1 (Tmf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592378.1

ORF Insert Sequence:

ATGAGCTGGT TCAATGCCTC GCAGTTGTCC AGCTTCGCCA AGCAGGCCCT GTCCCAGGCC 
CAGAAGTCCA TCGACAGGGT CCTGGACATC CAGGAGGAAG AGCCGAGCGC CTGGGCCGAG 
GCCATCCCGT ACGGTGAGCC AGGAATAAGT CCCTCTGTGA GTGGAGGATG GGATACTTCA 
ACCTGGGGAT TGAACTCAAG CTCTGAACCT CAGAGTTCAC CAACAGCCTC TCAAGCAATT 
ACAAAGCCAG TTCGGAGGAC TGTAGTAGAT GAATCTGAAA ATTTCTTCAG TGCCTTTCTC 
TCTCCATCGG ATGTGCAGAC CATTCAGAAG AGTCCAGTGG TCTCAAAGCC TCCTGCTAAA 
TCACAGCGAC CAGAAGAAGA AGTGAAAAGC AGTTTACAAG AACCATCGTC TCCTGGCCAG 
TCCAGAACCT CTGAGACAGC GGAGTCTGAA GCGACAGACT CTGCCTGTGT TTCGGGAGAG 
ACTCCAGCCG TGGGCACTCC CTCACCTGTC CCTGAAGGCA AGCGTGAAGA AACTGCTAGT 
GAAGAGTCTG AGGGGAAGGT GCCCACCGTG AGAGCGAAGG CGTCTGAAAG CGTAGTTAAG 
GTAAACACAG CAGAAGATGT ATCTCCCACA TCCACTCAGT CTCTTACGAC CGAAACGAAG 
GATATGGCTT TGGAGCCTAA AGAACAAAAA CACGAAGACA GACAAAGCAA CACACCTTCT 
CCTCCTGTTA GCTCCTTCTC GTCAGGCACT TCCACCACCA GTGACATTGA AGTTTTAGAT 
CATGAAAGTG TAATAAGTGA GAGCTCAGCT AGTTCAAGGC AAGAGACTTC AGATGCAAAG 
TCAAGTCTTC ACCTCATGCA GACCTCATTT CAGCTTCTCT CTGCATCTGC TTGTCCTGAG 
TATGGCCGCT TAGATGACTT CCAAAAGCTC AGTGAGAGTT GCTGCTCTTC GGATGCTTTT 
GAGAGAATAG ACTCGTTTAG CGTGCAGTCT CTGGATAGCC GGAGTGTGAG TGAAATCAAC 
TCGGACGACG AACTGCCGGG TAGAGGCTAT GCTTTAGTAC CTATAGTCGT GAGTCCTTCA 
GCCCCGAAGA CAAAAGTAGT AGAATCTACT GAGGAAAACG TTGAAGAAGA GGAAGGAAAC 
GAGACATTAA TTGCACCTTC TGAGGAAGCA GAATTAGAGG AAAGTGGCCG AAGTGCTACT 
CCAGTTAACT GTGACCAGCC AGATACATTG GCTTCTCCTA CACCAGTAAA TGTTGGGCCC 
TCTGCCTCTG GACCGGCCGC TGAGGATGGT GAAGCTGCTG AAAACCACCC AAAAGCACCA 
CAGCTGGAGC AAGAAGATGT TGGCAAGACA GTTGAATTTC TGAATGAGAA GCTAGAAAAG 
AGGGAGACTC AATTATTGTC ACTTAGTAAG GAAAAGGCAC TTCTAGAGGA AGCTTATGAT 
AACCTGAAGG ATGAAATGTT CAGAGTAAAA GAAGAAAGTA GTAGCATTTC TTCCCTGAAA 
GATGAGTTTA CCCAGAGGAT CGCAGAAGCA GAAAGGAAAG CTCAACTCGC CTGCAAAGAG 
AGAGACACTG CGAAGAAGGA AATGAAAACC ATGAAGGAAG AACTTGCCTC TAGATTAAAT 
AGTAGTCAGA CTGCAGACCT TCTGAAGGAA AAGGATGAAC AGATTCAAGG CTTAATGGAA 
GAAGGAGAAA AACTTTCGAA GCAGCAGCTG CATAATTCCA ACATGATTAA GAAATTAAGA 
GCTAAGGATA AAGACAATGA AAGTGTTATT GCAAAGCTGA AGAAAAAAGC TAAGGAGCTG 
GAAGAGGAGC TACAGCATTT AAGACAGGTC CTTGATGGCA AAGAAGAGGT TGAGAAACAA 
CATAGAGAAA ACATTAAAAA ACTAAATTCT GTAGTAGAAC GCCAGGAAAA AGATCTTGGC 
CGACTTCAGG TAGACATGAA TGAACTTGAA GAAAAGAGCC GAAGTACTCA GGCTGCCCTA 
GACAGTGCCT ACAGAGAACT TACTGATCTT CACAAAGCCA ACGCTGCTAA GGACAGTGAG 
GTACAAGAAG CTGCTCTGAG GCGTGAGATG AAAGCTAAAG AAGAACTTTC TGGGGCACTG 
GAGAAGGCCC AGGAAGAAGC CCGTCAGCAG CAAGAGACTT TAGCACTTCA GGTTGGGGAC 
CTTAGGCTAG CGCTGCAACG GGCAGAGCAA GCAGCTGCCA GGAAAGAGGA TTACTTACGC 
CATGAGATCA GTGAACTTCA GCAGAGACTC CAAGAAGCAG AGAATCGAAA CCAGGAGCTG 
AGTCAAAGTG TTTCATCAAC AACAAGACCT TTGCTTCGAC AGATAGAAAA TTTACAAGCA 
ACTCTAGGAT CCCAGACATC GTCATGGGAG ATGCTAGAGA AGAATCTTTC TGATCGGCTC 
GGTGAATCAC AGACCTTGTT GGCTGCAGCA GTTGAAAGAG AACGTGCAGC TACGGAAGAA 
CTCCTAGCCA ACAAAATCCA GATGTCTTCA GTGGAGTCGC AGAATGCATT ACTACGACAG 
GAAAACAGTA GACTGCAGGC CCAGCTAGAG TCAGAGAAAA ATAAATTAAG AAAACTAGAG 
GATGAAAATA GCCGGTACCA GGTTGAATTA GAAAATCTAA AAGATGAATA TGTAAGAACA 
CTTGAAGAGG CAAGGAAAGA AAAGACACTT TTGAGCAGTC AGTTAGAAAT GGAAAGAATG 
AAAGTTGAAC AAGAAAGGAA GAAAACCATT TTCACTCAAG AAACACTAAA AGAAAAGTCT 
TACAGAGTTT TTTTCCCCCT AAGGAGCACA AGCCGTTTTC CGTTTCTAGT ACTCCCACCA 
TGTCACGATC CAGTTCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmf1 ( NM_053671.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446123.2 Rattus norvegicus TATA element modulatory factor 1 (Tmf1), mRNA.

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CloneID	ORa10275
Clone ID Related Accession (Same CDS sequence)	NM_053671.2
Accession Version	NM_053671.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	TATA element modulatory factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BF415257.1, CK470592.1, AF107843.1, CK473511.1, DY564782.1, CK602135.1, BQ780433.1 and BM390672.1. On Oct 12, 2007 this sequence version replaced NM_053671.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGCTGGT TCAATGCCTC GCAGTTGTCC AGCTTCGCCA AGCAGGCCCT GTCCCAGGCC 
CAGAAGTCCA TCGACAGGGT CCTGGACATC CAGGAGGAAG AGCCGAGCGC CTGGGCCGAG 
GCCATCCCGT ACGGTGAGCC AGGAATAAGT CCCTCTGTGA GTGGAGGATG GGATACTTCA 
ACCTGGGGAT TGAACTCAAG CTCTGAACCT CAGAGTTCAC CAACAGCCTC TCAAGCAATT 
ACAAAGCCAG TTCGGAGGAC TGTAGTAGAT GAATCTGAAA ATTTCTTCAG TGCCTTTCTC 
TCTCCATCGG ATGTGCAGAC CATTCAGAAG AGTCCAGTGG TCTCAAAGCC TCCTGCTAAA 
TCACAGCGAC CAGAAGAAGA AGTGAAAAGC AGTTTACAAG AACCATCGTC TCCTGGCCAG 
TCCAGAACCT CTGAGACAGC GGAGTCTGAA GCGACAGACT CTGCCTGTGT TTCGGGAGAG 
ACTCCAGCCG TGGGCACTCC CTCACCTGTC CCTGAAGGCA AGCGTGAAGA AACTGCTAGT 
GAAGAGTCTG AGGGGAAGGT GCCCACCGTG AGAGCGAAGG CGTCTGAAAG CGTAGTTAAG 
GTAAACACAG CAGAAGATGT ATCTCCCACA TCCACTCAGT CTCTTACGAC CGAAACGAAG 
GATATGGCTT TGGAGCCTAA AGAACAAAAA CACGAAGACA GACAAAGCAA CACACCTTCT 
CCTCCTGTTA GCTCCTTCTC GTCAGGCACT TCCACCACCA GTGACATTGA AGTTTTAGAT 
CATGAAAGTG TAATAAGTGA GAGCTCAGCT AGTTCAAGGC AAGAGACTTC AGATGCAAAG 
TCAAGTCTTC ACCTCATGCA GACCTCATTT CAGCTTCTCT CTGCATCTGC TTGTCCTGAG 
TATGGCCGCT TAGATGACTT CCAAAAGCTC AGTGAGAGTT GCTGCTCTTC GGATGCTTTT 
GAGAGAATAG ACTCGTTTAG CGTGCAGTCT CTGGATAGCC GGAGTGTGAG TGAAATCAAC 
TCGGACGACG AACTGCCGGG TAGAGGCTAT GCTTTAGTAC CTATAGTCGT GAGTCCTTCA 
GCCCCGAAGA CAAAAGTAGT AGAATCTACT GAGGAAAACG TTGAAGAAGA GGAAGGAAAC 
GAGACATTAA TTGCACCTTC TGAGGAAGCA GAATTAGAGG AAAGTGGCCG AAGTGCTACT 
CCAGTTAACT GTGACCAGCC AGATACATTG GCTTCTCCTA CACCAGTAAA TGTTGGGCCC 
TCTGCCTCTG GACCGGCCGC TGAGGATGGT GAAGCTGCTG AAAACCACCC AAAAGCACCA 
CAGCTGGAGC AAGAAGATGT TGGCAAGACA GTTGAATTTC TGAATGAGAA GCTAGAAAAG 
AGGGAGACTC AATTATTGTC ACTTAGTAAG GAAAAGGCAC TTCTAGAGGA AGCTTATGAT 
AACCTGAAGG ATGAAATGTT CAGAGTAAAA GAAGAAAGTA GTAGCATTTC TTCCCTGAAA 
GATGAGTTTA CCCAGAGGAT CGCAGAAGCA GAAAGGAAAG CTCAACTCGC CTGCAAAGAG 
AGAGACACTG CGAAGAAGGA AATGAAAACC ATGAAGGAAG AACTTGCCTC TAGATTAAAT 
AGTAGTCAGA CTGCAGACCT TCTGAAGGAA AAGGATGAAC AGATTCAAGG CTTAATGGAA 
GAAGGAGAAA AACTTTCGAA GCAGCAGCTG CATAATTCCA ACATGATTAA GAAATTAAGA 
GCTAAGGATA AAGACAATGA AAGTGTTATT GCAAAGCTGA AGAAAAAAGC TAAGGAGCTG 
GAAGAGGAGC TACAGCATTT AAGACAGGTC CTTGATGGCA AAGAAGAGGT TGAGAAACAA 
CATAGAGAAA ACATTAAAAA ACTAAATTCT GTAGTAGAAC GCCAGGAAAA AGATCTTGGC 
CGACTTCAGG TAGACATGAA TGAACTTGAA GAAAAGAGCC GAAGTACTCA GGCTGCCCTA 
GACAGTGCCT ACAGAGAACT TACTGATCTT CACAAAGCCA ACGCTGCTAA GGACAGTGAG 
GTACAAGAAG CTGCTCTGAG GCGTGAGATG AAAGCTAAAG AAGAACTTTC TGGGGCACTG 
GAGAAGGCCC AGGAAGAAGC CCGTCAGCAG CAAGAGACTT TAGCACTTCA GGTTGGGGAC 
CTTAGGCTAG CGCTGCAACG GGCAGAGCAA GCAGCTGCCA GGAAAGAGGA TTACTTACGC 
CATGAGATCA GTGAACTTCA GCAGAGACTC CAAGAAGCAG AGAATCGAAA CCAGGAGCTG 
AGTCAAAGTG TTTCATCAAC AACAAGACCT TTGCTTCGAC AGATAGAAAA TTTACAAGCA 
ACTCTAGGAT CCCAGACATC GTCATGGGAG ATGCTAGAGA AGAATCTTTC TGATCGGCTC 
GGTGAATCAC AGACCTTGTT GGCTGCAGCA GTTGAAAGAG AACGTGCAGC TACGGAAGAA 
CTCCTAGCCA ACAAAATCCA GATGTCTTCA GTGGAGTCGC AGAATGCATT ACTACGACAG 
GAAAACAGTA GACTGCAGGC CCAGCTAGAG TCAGAGAAAA ATAAATTAAG AAAACTAGAG 
GATGAAAATA GCCGGTACCA GGTTGAATTA GAAAATCTAA AAGATGAATA TGTAAGAACA 
CTTGAAGAGG CAAGGAAAGA AAAGACACTT TTGAGCAGTC AGTTAGAAAT GGAAAGAATG 
AAAGTTGAAC AAGAAAGGAA GAAAACCATT TTCACTCAAG AAACACTAAA AGAAAAGGAG 
CACAAGCCGT TTTCCGTTTC TAGTACTCCC ACCATGTCAC GATCCAGTTC TCTAAGTGGA 
GTAGATATGG CAGGGCTGCA GACATCCTTC CTATCTCAGG ATGAGTCTCA CGACCATTCA 
TTTGGGCCAA TGTCTACGTC AGCCAGTGGA AGCAATCTTT ATGAAGCTGT AAGGATGGGA 
GCCGGATCAA GCATTATCGA AAATCTTCAG TCTCAGCTAA AGCTGAGGGA AGGAGAAATT 
AGTCATTTAC AGCTAGAAAT TAGTAACCTA GAAAAAACTC GATCCATAAT GTCCGAAGAG 
CTAGTTAAAT TAACAAATCA GAATGATGAA CTTGAGGAGA AAGTGAAAGA GATACCCAAG 
CTTCGAGTTC AGCTGCGAGA TTTGGATCAA AGATACAACA CTATTCTGCA GATGTATGGA 
GAAAAAGCAG AAGAAGCAGA GGAACTTCGC CTGGATCTTG AAGATGTAAA AAATATGTAT 
AAAACTCAAA TAGATGAACT TCTAAGACAA AGACTCAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446123.2
CDS	78..3359
Translation	MSWFNASQLSSFAKQALSQAQKSIDRVLDIQEEEPSAWAEAIPYGEPGISPSVSGGWDTSTWGLNSSSEPQSSPTASQAITKPVRRTVVDESENFFSAFLSPSDVQTIQKSPVVSKPPAKSQRPEEEVKSSLQEPSSPGQSRTSETAESEATDSACVSGETPAVGTPSPVPEGKREETASEESEGKVPTVRAKASESVVKVNTAEDVSPTSTQSLTTETKDMALEPKEQKHEDRQSNTPSPPVSSFSSGTSTTSDIEVLDHESVISESSASSRQETSDAKSSLHLMQTSFQLLSASACPEYGRLDDFQKLSESCCSSDAFERIDSFSVQSLDSRSVSEINSDDELPGRGYALVPIVVSPSAPKTKVVESTEENVEEEEGNETLIAPSEEAELEESGRSATPVNCDQPDTLASPTPVNVGPSASGPAAEDGEAAENHPKAPQLEQEDVGKTVEFLNEKLEKRETQLLSLSKEKALLEEAYDNLKDEMFRVKEESSSISSLKDEFTQRIAEAERKAQLACKERDTAKKEMKTMKEELASRLNSSQTADLLKEKDEQIQGLMEEGEKLSKQQLHNSNMIKKLRAKDKDNESVIAKLKKKAKELEEELQHLRQVLDGKEEVEKQHRENIKKLNSVVERQEKDLGRLQVDMNELEEKSRSTQAALDSAYRELTDLHKANAAKDSEVQEAALRREMKAKEELSGALEKAQEEARQQQETLALQVGDLRLALQRAEQAAARKEDYLRHEISELQQRLQEAENRNQELSQSVSSTTRPLLRQIENLQATLGSQTSSWEMLEKNLSDRLGESQTLLAAAVERERAATEELLANKIQMSSVESQNALLRQENSRLQAQLESEKNKLRKLEDENSRYQVELENLKDEYVRTLEEARKEKTLLSSQLEMERMKVEQERKKTIFTQETLKEKEHKPFSVSSTPTMSRSSSLSGVDMAGLQTSFLSQDESHDHSFGPMSTSASGSNLYEAVRMGAGSSIIENLQSQLKLREGEISHLQLEISNLEKTRSIMSEELVKLTNQNDELEEKVKEIPKLRVQLRDLDQRYNTILQMYGEKAEEAEELRLDLEDVKNMYKTQIDELLRQRLS

Target ORF information:

RefSeq Version	NM_053671.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TATA element modulatory factor 1 (Tmf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053671.2

ORF Insert Sequence:

ATGAGCTGGT TCAATGCCTC GCAGTTGTCC AGCTTCGCCA AGCAGGCCCT GTCCCAGGCC 
CAGAAGTCCA TCGACAGGGT CCTGGACATC CAGGAGGAAG AGCCGAGCGC CTGGGCCGAG 
GCCATCCCGT ACGGTGAGCC AGGAATAAGT CCCTCTGTGA GTGGAGGATG GGATACTTCA 
ACCTGGGGAT TGAACTCAAG CTCTGAACCT CAGAGTTCAC CAACAGCCTC TCAAGCAATT 
ACAAAGCCAG TTCGGAGGAC TGTAGTAGAT GAATCTGAAA ATTTCTTCAG TGCCTTTCTC 
TCTCCATCGG ATGTGCAGAC CATTCAGAAG AGTCCAGTGG TCTCAAAGCC TCCTGCTAAA 
TCACAGCGAC CAGAAGAAGA AGTGAAAAGC AGTTTACAAG AACCATCGTC TCCTGGCCAG 
TCCAGAACCT CTGAGACAGC GGAGTCTGAA GCGACAGACT CTGCCTGTGT TTCGGGAGAG 
ACTCCAGCCG TGGGCACTCC CTCACCTGTC CCTGAAGGCA AGCGTGAAGA AACTGCTAGT 
GAAGAGTCTG AGGGGAAGGT GCCCACCGTG AGAGCGAAGG CGTCTGAAAG CGTAGTTAAG 
GTAAACACAG CAGAAGATGT ATCTCCCACA TCCACTCAGT CTCTTACGAC CGAAACGAAG 
GATATGGCTT TGGAGCCTAA AGAACAAAAA CACGAAGACA GACAAAGCAA CACACCTTCT 
CCTCCTGTTA GCTCCTTCTC GTCAGGCACT TCCACCACCA GTGACATTGA AGTTTTAGAT 
CATGAAAGTG TAATAAGTGA GAGCTCAGCT AGTTCAAGGC AAGAGACTTC AGATGCAAAG 
TCAAGTCTTC ACCTCATGCA GACCTCATTT CAGCTTCTCT CTGCATCTGC TTGTCCTGAG 
TATGGCCGCT TAGATGACTT CCAAAAGCTC AGTGAGAGTT GCTGCTCTTC GGATGCTTTT 
GAGAGAATAG ACTCGTTTAG CGTGCAGTCT CTGGATAGCC GGAGTGTGAG TGAAATCAAC 
TCGGACGACG AACTGCCGGG TAGAGGCTAT GCTTTAGTAC CTATAGTCGT GAGTCCTTCA 
GCCCCGAAGA CAAAAGTAGT AGAATCTACT GAGGAAAACG TTGAAGAAGA GGAAGGAAAC 
GAGACATTAA TTGCACCTTC TGAGGAAGCA GAATTAGAGG AAAGTGGCCG AAGTGCTACT 
CCAGTTAACT GTGACCAGCC AGATACATTG GCTTCTCCTA CACCAGTAAA TGTTGGGCCC 
TCTGCCTCTG GACCGGCCGC TGAGGATGGT GAAGCTGCTG AAAACCACCC AAAAGCACCA 
CAGCTGGAGC AAGAAGATGT TGGCAAGACA GTTGAATTTC TGAATGAGAA GCTAGAAAAG 
AGGGAGACTC AATTATTGTC ACTTAGTAAG GAAAAGGCAC TTCTAGAGGA AGCTTATGAT 
AACCTGAAGG ATGAAATGTT CAGAGTAAAA GAAGAAAGTA GTAGCATTTC TTCCCTGAAA 
GATGAGTTTA CCCAGAGGAT CGCAGAAGCA GAAAGGAAAG CTCAACTCGC CTGCAAAGAG 
AGAGACACTG CGAAGAAGGA AATGAAAACC ATGAAGGAAG AACTTGCCTC TAGATTAAAT 
AGTAGTCAGA CTGCAGACCT TCTGAAGGAA AAGGATGAAC AGATTCAAGG CTTAATGGAA 
GAAGGAGAAA AACTTTCGAA GCAGCAGCTG CATAATTCCA ACATGATTAA GAAATTAAGA 
GCTAAGGATA AAGACAATGA AAGTGTTATT GCAAAGCTGA AGAAAAAAGC TAAGGAGCTG 
GAAGAGGAGC TACAGCATTT AAGACAGGTC CTTGATGGCA AAGAAGAGGT TGAGAAACAA 
CATAGAGAAA ACATTAAAAA ACTAAATTCT GTAGTAGAAC GCCAGGAAAA AGATCTTGGC 
CGACTTCAGG TAGACATGAA TGAACTTGAA GAAAAGAGCC GAAGTACTCA GGCTGCCCTA 
GACAGTGCCT ACAGAGAACT TACTGATCTT CACAAAGCCA ACGCTGCTAA GGACAGTGAG 
GTACAAGAAG CTGCTCTGAG GCGTGAGATG AAAGCTAAAG AAGAACTTTC TGGGGCACTG 
GAGAAGGCCC AGGAAGAAGC CCGTCAGCAG CAAGAGACTT TAGCACTTCA GGTTGGGGAC 
CTTAGGCTAG CGCTGCAACG GGCAGAGCAA GCAGCTGCCA GGAAAGAGGA TTACTTACGC 
CATGAGATCA GTGAACTTCA GCAGAGACTC CAAGAAGCAG AGAATCGAAA CCAGGAGCTG 
AGTCAAAGTG TTTCATCAAC AACAAGACCT TTGCTTCGAC AGATAGAAAA TTTACAAGCA 
ACTCTAGGAT CCCAGACATC GTCATGGGAG ATGCTAGAGA AGAATCTTTC TGATCGGCTC 
GGTGAATCAC AGACCTTGTT GGCTGCAGCA GTTGAAAGAG AACGTGCAGC TACGGAAGAA 
CTCCTAGCCA ACAAAATCCA GATGTCTTCA GTGGAGTCGC AGAATGCATT ACTACGACAG 
GAAAACAGTA GACTGCAGGC CCAGCTAGAG TCAGAGAAAA ATAAATTAAG AAAACTAGAG 
GATGAAAATA GCCGGTACCA GGTTGAATTA GAAAATCTAA AAGATGAATA TGTAAGAACA 
CTTGAAGAGG CAAGGAAAGA AAAGACACTT TTGAGCAGTC AGTTAGAAAT GGAAAGAATG 
AAAGTTGAAC AAGAAAGGAA GAAAACCATT TTCACTCAAG AAACACTAAA AGAAAAGGAG 
CACAAGCCGT TTTCCGTTTC TAGTACTCCC ACCATGTCAC GATCCAGTTC TCTAAGTGGA 
GTAGATATGG CAGGGCTGCA GACATCCTTC CTATCTCAGG ATGAGTCTCA CGACCATTCA 
TTTGGGCCAA TGTCTACGTC AGCCAGTGGA AGCAATCTTT ATGAAGCTGT AAGGATGGGA 
GCCGGATCAA GCATTATCGA AAATCTTCAG TCTCAGCTAA AGCTGAGGGA AGGAGAAATT 
AGTCATTTAC AGCTAGAAAT TAGTAACCTA GAAAAAACTC GATCCATAAT GTCCGAAGAG 
CTAGTTAAAT TAACAAATCA GAATGATGAA CTTGAGGAGA AAGTGAAAGA GATACCCAAG 
CTTCGAGTTC AGCTGCGAGA TTTGGATCAA AGATACAACA CTATTCTGCA GATGTATGGA 
GAAAAAGCAG AAGAAGCAGA GGAACTTCGC CTGGATCTTG AAGATGTAAA AAATATGTAT 
AAAACTCAAA TAGATGAACT TCTAAGACAA AGACTCAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Testosterone deficiency accompanied by testicular and epididymal abnormalities in TMF(-/-) mice.
Molecular and cellular endocrinology365(1)52-63(2013 Jan)
Elkis Y,Bel S,Lerer-Goldstein T,Nyska A,Creasy DM,Shpungin S,Nir U

Loss of TMF/ARA160 protein renders colonic mucus refractory to bacterial colonization and diminishes intestinal susceptibility to acute colitis.
The Journal of biological chemistry287(30)25631-9(2012 Jul)
Bel S,Elkis Y,Lerer-Goldstein T,Nyska A,Shpungin S,Nir U

CTC1 deletion results in defective telomere replication, leading to catastrophic telomere loss and stem cell exhaustion.
The EMBO journal31(10)2309-21(2012 May)
Gu P,Min JN,Wang Y,Huang C,Peng T,Chai W,Chang S

TMF/ARA160: A key regulator of sperm development.
Developmental biology348(1)12-21(2010 Dec)
Lerer-Goldshtein T,Bel S,Shpungin S,Pery E,Motro B,Goldstein RS,Bar-Sheshet SI,Breitbart H,Nir U

trnp: A conserved mammalian gene encoding a nuclear protein that accelerates cell-cycle progression.
DNA and cell biology25(6)331-9(2006 Jun)
Volpe M,Shpungin S,Barbi C,Abrham G,Malovani H,Wides R,Nir U

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Tmf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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