P3h1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	P3h1
Entrez Gene ID	114200
Full Name	prolyl 3-hydroxylase 1
Synonyms	Gros1,Lepre1
General protein information	Preferred Names prolyl 3-hydroxylase 1 Names prolyl 3-hydroxylase 1 growth supressor 1 leprecan-1 leucine proline-enriched proteoglycan (leprecan) 1 leucine- and proline-enriched proteoglycan 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	basement membrane-associated proteoglycan; may play a role in the secretory pathway of cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053667.1	NP_446119.1	prolyl 3-hydroxylase 1 precursor

Pathways from BioSystems

Homologies

	LEPRE1	XP_001088904.1
Mus musculus (house mouse)	Lepre1	NP_062757.2
Homo sapiens (human)	LEPRE1	NP_071751.3
Rattus norvegicus (Norway rat)	Lepre1	NP_446119.1
Gallus gallus (chicken)	LEPRE1	NP_001001529.1
Danio rerio (zebrafish)	lepre1	XP_002663865.1
Xenopus tropicalis (tropical clawed frog)	lepre1	NP_001090700.1
Pan troglodytes (chimpanzee)	LEPRE1	XP_003308086.1
Canis lupus familiaris (dog)	LEPRE1	XP_848570.2
Bos taurus (cattle)	LEPRE1	NP_001096761.1

Gene Ontology
Bibliography

Related articles in PubMed

A multilevel screening strategy defines a molecular fingerprint of proregenerative olfactory ensheathing cells and identifies SCARB2, a protein that improves regenerative sprouting of injured sensory spinal axons.
Roet KC, Franssen EH, de Bree FM, Essing AH, Zijlstra SJ, Fagoe ND, Eggink HM, Eggers R, Smit AB, van Kesteren RE, Verhaagen J
The Journal of neuroscience : the official journal of the Society for Neuroscience33(27)11116-35(2013 Jul)

A novel mutation in LEPRE1 that eliminates only the KDEL ER- retrieval sequence causes non-lethal osteogenesis imperfecta.
Takagi M, Ishii T, Barnes AM, Weis M, Amano N, Tanaka M, Fukuzawa R, Nishimura G, Eyre DR, Marini JC, Hasegawa T
PloS one7(5)e36809(2012)

Prolyl 3-hydroxylase 1 null mice display abnormalities in fibrillar collagen-rich tissues such as tendons, skin, and bones.
Vranka JA, Pokidysheva E, Hayashi L, Zientek K, Mizuno K, Ishikawa Y, Maddox K, Tufa S, Keene DR, Klein R, B?chinger HP
The Journal of biological chemistry285(22)17253-62(2010 May)

Lack of cyclophilin B in osteogenesis imperfecta with normal collagen folding.
Barnes AM, Carter EM, Cabral WA, Weis M, Chang W, Makareeva E, Leikin S, Rotimi CN, Eyre DR, Raggio CL, Marini JC
The New England journal of medicine362(6)521-8(2010 Feb)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Regulated expression of leprecan during glomerular development or hypertrophy coupled with its reported prolyl hydroxylase activity plays a role during basement membrane assembly.
Title: Leprecan distribution in the developing and adult kidney.

The following P3h1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P3h1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10264	NM_053667.1 Latest version!	Rattus norvegicus prolyl 3-hydroxylase 1 (P3h1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P3h1 ( NM_053667.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446119.1 Rattus norvegicus prolyl 3-hydroxylase 1 (P3h1), mRNA.

Price & Availability↑

CloneID	ORa10264
Clone ID Related Accession (Same CDS sequence)	NM_053667.1
Accession Version	NM_053667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	prolyl 3-hydroxylase 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF087433.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF087433.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTAGCGG TCGCCGCAGC CGCCGCCTCG CGGGCTACCG CGGAGTCTGA GCCGGAATGG 
AACGTGGCAG CCCCTGACCT ACTTTACGCA GAGGGGACGG CGGCCTACGC GCGCGGGGAC 
TGGCCCGGGG TGGTACTGAA CATGGAGCGG GCTCTGCGCT CGCGGGCCGC TCTGCGCGCC 
CTCCGCCTGC GCTGCCGCAC ACGCTGCGCC ACCGAGCTGC CGTGGGCGCC GGACCTCGAT 
CTCGGTCCGG CCTCGAGTCT GAACCACGAC CCGGGCGCTG CCGCCTTGCA CGACCTTCGC 
TTCTTCGGAG CCCTGCTGCG CCGTGCCGCC TGCCTGCGTC GCTGCCTCGG GCCGCCTTCT 
GCCCACTTGT TGAGTGAAGA GCTGGACCTG GAGTTCAACA AGCGGAGCCC GTACAACTAC 
CTGCAGGTCG CCTATTTCAA GATAAACAAG CTGGAAAAAG CCGTGGCAGC AGCACACACC 
TTCTTTGTGG GCAATCCTGA GCACATGGAA ATGCGGCAGA ACCTCGACTA CTACCAAACC 
ATGTCTGGGG TAAAGGAGGA AGACTTCAAG GACCTCGAGG CCAAGCCCCA CATGCATGAG 
TTTCGGCTAG GGGTGCGACT CTACTCTGAG GAGAAGCCAC TGGAAGCTGT GCCCCACCTG 
GAGGCGGCGT TGCAAGAGTA CTTTGTGGCA GATGAGGAGT GCCGTGCCCT CTGCGAAGGG 
CCCTATGACT ACGACGGCTA CAACTACCTA GACTACAGCG CTGACCTCTT CCAGGCCATC 
ACAGATCATT ACGTCCAGGT CCTCAGCTGT AAGCAGAACT GTGTCACCGA GCTGGCTTCC 
CACCCAAGTC GAGAAAAGCC TTTTGAAGAC TTCCTCCCCT CACACTATAA TTACCTACAG 
TTTGCCTACT ATAACATTGG GAACTATACA CAAGCTATTG AATGTGCCAA GACCTACCTC 
CTCTTCTTCC CCAATGATGA GGTGATGAGT CAGAACCTGG CTTACTATAC AGCCGTGCTT 
GGAGAAGAGG AGGCCAGCTC CATCAGTCCC CGGGAGAATG CCCAGGAATA CCGACATCGA 
AGCCTGCTGG AGAAAGAACT GCTTTTCTTC GCTTATGACA TTTTCGGAAT TCCCTTTGTG 
GATCCGGACT CATGGACACC AGAAGAGGTG ATTCCCAAGA GACTGCAAGA GAAACAAAAG 
TCAGAACGCG AAACCGCAGT CCGCATCTCC CAGGAGATAG GCAACCTCAT GAAGGAGATC 
GAGACCCTCG TGGAGGAGAA GACGAAGGAG TCGCTGGATG TGAGCAGACT GACACGGGAA 
GGTGGTCCCT TACTGTATGA AGGCATCAAT CTCACTATGA ACTCCAAAGT CTTGAACGGC 
TCCCAGCGAG TGGTGATGGA TGGTGTGATC TCTGATGACG AGTGCCAGGA ACTGCAGAGA 
CTGACCAACG CAGCAGCAAC TTCAGGAGAT GGCTATCGAG GTCAGACCTC CCCACACACC 
CCAAATGAAA AGTTCTATGG TGTCACTGTC CTCAAAGCTC TCAAGCTTGG GCAGGAAGGG 
AAAGTTCCTC TGCAGAGCGC CCACATGTAC TACAACGTGA CAGAGAAGGT TCGGCGTGTC 
ATGGAGTCCT ACTTCCGCCT GGACACACCC CTCTACTTCT CCTACTCCCA CCTAGTGTGC 
CGCACTGCAA TAGAAGAGTC ACAGGCTGAG AGGAAGGACA GTAGCCACCC TGTCCATGTG 
GATAACTGCA TTCTGAATGC CGAATCCCTC GTGTGTATCA AGGAGCCCCC AGCCTATACG 
TTCCGGGACT ACAGCGCCAT CCTCTACCTC AATGGGGACT TCGATGGAGG AAACTTCTAC 
TTCACAGAAC TAGATGCCAA GACTGTGACG GCAGAGGTGC AGCCCCAGTG TGGAAGGGCT 
GTGGGATTCT CTTCCGGCAC CGAGAACCCA CATGGAGTGA AGGCTGTCAC CAGGGGGCAG 
CGCTGCGCCA TCGCCCTGTG GTTCACGCTG GATCCTCGGC ACAGTGAGAG AGACAGGGTA 
CAGGCAGATG ACCTGGTGAA GATGCTCTTC AGCCCAGAAG AGGTGGACCT CCCCCAGGAG 
CAGCCCCTGC CTGACCAACA GGGCTCCCCG AAACCTGGAG AAGAGTCTCT GTCCGACAGA 
GAGTCACAGC CCAAGGATGA GCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446119.1
CDS	14..2200
Translation	MVAVAAAAASRATAESEPEWNVAAPDLLYAEGTAAYARGDWPGVVLNMERALRSRAALRALRLRCRTRCATELPWAPDLDLGPASSLNHDPGAAALHDLRFFGALLRRAACLRRCLGPPSAHLLSEELDLEFNKRSPYNYLQVAYFKINKLEKAVAAAHTFFVGNPEHMEMRQNLDYYQTMSGVKEEDFKDLEAKPHMHEFRLGVRLYSEEKPLEAVPHLEAALQEYFVADEECRALCEGPYDYDGYNYLDYSADLFQAITDHYVQVLSCKQNCVTELASHPSREKPFEDFLPSHYNYLQFAYYNIGNYTQAIECAKTYLLFFPNDEVMSQNLAYYTAVLGEEEASSISPRENAQEYRHRSLLEKELLFFAYDIFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVRISQEIGNLMKEIETLVEEKTKESLDVSRLTREGGPLLYEGINLTMNSKVLNGSQRVVMDGVISDDECQELQRLTNAAATSGDGYRGQTSPHTPNEKFYGVTVLKALKLGQEGKVPLQSAHMYYNVTEKVRRVMESYFRLDTPLYFSYSHLVCRTAIEESQAERKDSSHPVHVDNCILNAESLVCIKEPPAYTFRDYSAILYLNGDFDGGNFYFTELDAKTVTAEVQPQCGRAVGFSSGTENPHGVKAVTRGQRCAIALWFTLDPRHSERDRVQADDLVKMLFSPEEVDLPQEQPLPDQQGSPKPGEESLSDRESQPKDEL

Target ORF information:

RefSeq Version	NM_053667.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prolyl 3-hydroxylase 1 (P3h1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053667.1

ORF Insert Sequence:

ATGGTAGCGG TCGCCGCAGC CGCCGCCTCG CGGGCTACCG CGGAGTCTGA GCCGGAATGG 
AACGTGGCAG CCCCTGACCT ACTTTACGCA GAGGGGACGG CGGCCTACGC GCGCGGGGAC 
TGGCCCGGGG TGGTACTGAA CATGGAGCGG GCTCTGCGCT CGCGGGCCGC TCTGCGCGCC 
CTCCGCCTGC GCTGCCGCAC ACGCTGCGCC ACCGAGCTGC CGTGGGCGCC GGACCTCGAT 
CTCGGTCCGG CCTCGAGTCT GAACCACGAC CCGGGCGCTG CCGCCTTGCA CGACCTTCGC 
TTCTTCGGAG CCCTGCTGCG CCGTGCCGCC TGCCTGCGTC GCTGCCTCGG GCCGCCTTCT 
GCCCACTTGT TGAGTGAAGA GCTGGACCTG GAGTTCAACA AGCGGAGCCC GTACAACTAC 
CTGCAGGTCG CCTATTTCAA GATAAACAAG CTGGAAAAAG CCGTGGCAGC AGCACACACC 
TTCTTTGTGG GCAATCCTGA GCACATGGAA ATGCGGCAGA ACCTCGACTA CTACCAAACC 
ATGTCTGGGG TAAAGGAGGA AGACTTCAAG GACCTCGAGG CCAAGCCCCA CATGCATGAG 
TTTCGGCTAG GGGTGCGACT CTACTCTGAG GAGAAGCCAC TGGAAGCTGT GCCCCACCTG 
GAGGCGGCGT TGCAAGAGTA CTTTGTGGCA GATGAGGAGT GCCGTGCCCT CTGCGAAGGG 
CCCTATGACT ACGACGGCTA CAACTACCTA GACTACAGCG CTGACCTCTT CCAGGCCATC 
ACAGATCATT ACGTCCAGGT CCTCAGCTGT AAGCAGAACT GTGTCACCGA GCTGGCTTCC 
CACCCAAGTC GAGAAAAGCC TTTTGAAGAC TTCCTCCCCT CACACTATAA TTACCTACAG 
TTTGCCTACT ATAACATTGG GAACTATACA CAAGCTATTG AATGTGCCAA GACCTACCTC 
CTCTTCTTCC CCAATGATGA GGTGATGAGT CAGAACCTGG CTTACTATAC AGCCGTGCTT 
GGAGAAGAGG AGGCCAGCTC CATCAGTCCC CGGGAGAATG CCCAGGAATA CCGACATCGA 
AGCCTGCTGG AGAAAGAACT GCTTTTCTTC GCTTATGACA TTTTCGGAAT TCCCTTTGTG 
GATCCGGACT CATGGACACC AGAAGAGGTG ATTCCCAAGA GACTGCAAGA GAAACAAAAG 
TCAGAACGCG AAACCGCAGT CCGCATCTCC CAGGAGATAG GCAACCTCAT GAAGGAGATC 
GAGACCCTCG TGGAGGAGAA GACGAAGGAG TCGCTGGATG TGAGCAGACT GACACGGGAA 
GGTGGTCCCT TACTGTATGA AGGCATCAAT CTCACTATGA ACTCCAAAGT CTTGAACGGC 
TCCCAGCGAG TGGTGATGGA TGGTGTGATC TCTGATGACG AGTGCCAGGA ACTGCAGAGA 
CTGACCAACG CAGCAGCAAC TTCAGGAGAT GGCTATCGAG GTCAGACCTC CCCACACACC 
CCAAATGAAA AGTTCTATGG TGTCACTGTC CTCAAAGCTC TCAAGCTTGG GCAGGAAGGG 
AAAGTTCCTC TGCAGAGCGC CCACATGTAC TACAACGTGA CAGAGAAGGT TCGGCGTGTC 
ATGGAGTCCT ACTTCCGCCT GGACACACCC CTCTACTTCT CCTACTCCCA CCTAGTGTGC 
CGCACTGCAA TAGAAGAGTC ACAGGCTGAG AGGAAGGACA GTAGCCACCC TGTCCATGTG 
GATAACTGCA TTCTGAATGC CGAATCCCTC GTGTGTATCA AGGAGCCCCC AGCCTATACG 
TTCCGGGACT ACAGCGCCAT CCTCTACCTC AATGGGGACT TCGATGGAGG AAACTTCTAC 
TTCACAGAAC TAGATGCCAA GACTGTGACG GCAGAGGTGC AGCCCCAGTG TGGAAGGGCT 
GTGGGATTCT CTTCCGGCAC CGAGAACCCA CATGGAGTGA AGGCTGTCAC CAGGGGGCAG 
CGCTGCGCCA TCGCCCTGTG GTTCACGCTG GATCCTCGGC ACAGTGAGAG AGACAGGGTA 
CAGGCAGATG ACCTGGTGAA GATGCTCTTC AGCCCAGAAG AGGTGGACCT CCCCCAGGAG 
CAGCCCCTGC CTGACCAACA GGGCTCCCCG AAACCTGGAG AAGAGTCTCT GTCCGACAGA 
GAGTCACAGC CCAAGGATGA GCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A multilevel screening strategy defines a molecular fingerprint of proregenerative olfactory ensheathing cells and identifies SCARB2, a protein that improves regenerative sprouting of injured sensory spinal axons.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(27)11116-35(2013 Jul)
Roet KC,Franssen EH,de Bree FM,Essing AH,Zijlstra SJ,Fagoe ND,Eggink HM,Eggers R,Smit AB,van Kesteren RE,Verhaagen J

A novel mutation in LEPRE1 that eliminates only the KDEL ER- retrieval sequence causes non-lethal osteogenesis imperfecta.
PloS one7(5)e36809(2012)
Takagi M,Ishii T,Barnes AM,Weis M,Amano N,Tanaka M,Fukuzawa R,Nishimura G,Eyre DR,Marini JC,Hasegawa T

Prolyl 3-hydroxylase 1 null mice display abnormalities in fibrillar collagen-rich tissues such as tendons, skin, and bones.
The Journal of biological chemistry285(22)17253-62(2010 May)
Vranka JA,Pokidysheva E,Hayashi L,Zientek K,Mizuno K,Ishikawa Y,Maddox K,Tufa S,Keene DR,Klein R,B?chinger HP

Lack of cyclophilin B in osteogenesis imperfecta with normal collagen folding.
The New England journal of medicine362(6)521-8(2010 Feb)
Barnes AM,Carter EM,Cabral WA,Weis M,Chang W,Makareeva E,Leikin S,Rotimi CN,Eyre DR,Raggio CL,Marini JC

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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P3h1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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