Akap1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Akap1
Entrez Gene ID	114124
Full Name	A-kinase anchoring protein 1
Synonyms	Akap84
General protein information	Preferred Names A-kinase anchoring protein 1 Names A-kinase anchor protein 1, mitochondrial A kinase (PRKA) anchor protein 1 A-kinase anchor protein 121 kDa AKAP 121 D-AKAP-1 PRKA1 S-AKAP84 a kinase anchor protein 1, mitochondrial dual specificity A-kinase-anchoring protein 1 protein kinase A-anchoring protein 1 spermatid A-kinase anchor protein 84
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26
Summary	scaffolding protein that immobilizes and concentrates protein kinase A II (PKAII) isoforms at specific intracellular locations [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006247068.1	XP_006247130.1	A-kinase anchor protein 1, mitochondrial isoform X1
XM_006247067.3	XP_006247129.1	A-kinase anchor protein 1, mitochondrial isoform X1
NM_053665.1	NP_446117.1	A-kinase anchor protein 1, mitochondrial
XM_008767930.1	XP_008766152.1	A-kinase anchor protein 1, mitochondrial isoform X1

Pathways from BioSystems

Homologies

	AKAP1	XP_001105339.1
Bos taurus (cattle)	AKAP1	XP_005220006.1
Mus musculus (house mouse)	Akap1	NP_033778.2
Pan troglodytes (chimpanzee)	AKAP1	XP_001172339.1
Canis lupus familiaris (dog)	AKAP1	XP_866604.2
Homo sapiens (human)	AKAP1	NP_001229832.1
Gallus gallus (chicken)	AKAP1	XP_415913.1
Rattus norvegicus (Norway rat)	Akap1	NP_446117.1

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Mechanism of neuroprotective mitochondrial remodeling by PKA/AKAP1.
Merrill RA, Dagda RK, Dickey AS, Cribbs JT, Green SH, Usachev YM, Strack S
PLoS biology9(4)e1000612(2011 Apr)

AKAP121 downregulation impairs protective cAMP signals, promotes mitochondrial dysfunction, and increases oxidative stress.
Perrino C, Feliciello A, Schiattarella GG, Esposito G, Guerriero R, Zaccaro L, Del Gatto A, Saviano M, Garbi C, Carangi R, Di Lorenzo E, Donato G, Indolfi C, Avvedimento VE, Chiariello M
Cardiovascular research88(1)101-10(2010 Oct)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the scaffold protein A kinase anchoring protein 1 brings the cAMP-dependent protein kinase A to the outer mitochondrial membrane to protect neurons from injury
Title: Mechanism of neuroprotective mitochondrial remodeling by PKA/AKAP1.

AKAP121 regulates the response to stress in cardiomyocytes, and therefore AKAP121 downregulation might represent an important event contributing to the development of cardiac dysfunction.
Title: AKAP121 downregulation impairs protective cAMP signals, promotes mitochondrial dysfunction, and increases oxidative stress.

These results are the first to identify AKAP121 as a negative regulator of cardiomyocyte hypertrophy.
Title: The A-kinase anchor protein AKAP121 is a negative regulator of cardiomyocyte hypertrophy.

Akap1 signaling complex mediates Adra1a-induced cardiomyocyte hypertrophy.
Title: The A-kinase anchoring protein (AKAP)-Lbc-signaling complex mediates alpha1 adrenergic receptor-induced cardiomyocyte hypertrophy.

90% or more of mitochondrial C-PKA, R-PKA and AKAP121 is localized in the inner mitochondrial compartment, when prepared both from isolated mitochondria or cardiomyocyte cultures
Title: Occurrence of A-kinase anchor protein and associated cAMP-dependent protein kinase in the inner compartment of mammalian mitochondria.

The following Akap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Akap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12846	NM_053665.1 Latest version!	Rattus norvegicus A-kinase anchoring protein 1 (Akap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$503.30 $719.00
ORa33547	XM_008767930.1 Latest version!	Rattus norvegicus A-kinase anchoring protein 1 (Akap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa33547	XM_006247067.3 Latest version!	Rattus norvegicus A-kinase anchoring protein 1 (Akap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa33547	XM_006247068.1 Latest version!	Rattus norvegicus A-kinase anchoring protein 1 (Akap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Akap1 ( NM_053665.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446117.1 Rattus norvegicus A-kinase anchoring protein 1 (Akap1), mRNA.

Price & Availability↑

CloneID	ORa12846
Clone ID Related Accession (Same CDS sequence)	NM_053665.1
Accession Version	NM_053665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein 1, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF068202.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF068202.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCAATCC AGTTCCGCTC ACTCTTCCCC TTGGCCTTGC CTGGAATGCT AGCACTCCTT 
GGCTGGTGGT GGTTTTTCTC TCGTAAAAAA GATCGGCTCA GCAGCAATGG CAAGCAGGTG 
GGGACATTGA AGGTTGGCCC TGCCATCGAG GACCGACTGC CCACTGAAGA GGCCTGTCCT 
GGAGTCCTGT CTGTGACCCC CAGTGTCACA CAGCCTCCTG GAAAGGAAGA GCAGCGCTCT 
ATGGACAGGC CTCTTTCAGA TCCCCCAGCC TTGCCAAGGA CCCGTCAAGT TCGCCGAAGA 
TCAGAGTCCT CAGGCAACCT CCCCAGCATT GTAGACACAA GGTTGCAGGC AGGACAGTGC 
AGCGATGAGA ATTCAAAAGT GGTACTCTCC CTGATGGGGG ACGAAGCCAA ATCTATTCCT 
CTTGGACGTC CGCTTTTCCC AAAGGATCTG TCCTTCCCCT ATGAAGCAGT GGAAGGGTGT 
AAGCAAGAGT CTGCCCTGGG CAGAACTCCT GGGAGAGGCT GGCTAAGCCA GTGTGCAGCC 
TCTGGAGAGA ACGCGAGAGA GACGGGTGGG GCGGAAGGGA CTGGAGATGC TGTGTTGGGG 
GAAAGTGTAC TTGAGGAAGG CCTATTGCCC CAGGAGTGTG TTTCAGAAGT AGAGAAGAGT 
GAGTTTCCGA TCCTAGCTCC CGGGGGAGGC GGGGGAGAGA AGGTGAGAAG TGGCCCACCA 
CAGGTAGATG AGCTTTTAAA GAAGGAAGAA TATATTGTTG GGAAGTTGCC AAGTAGCTTT 
GTGGGGCCAG TTCACTCGGA GCTGGTTAAG GATGAGGGTG CGCTGGTACC CCAAGTCAAA 
GGTAGCCAGG ACAGAAGCCT GGCTAGAGAA CTGGACAAAG ACAAGACCTT GCCTGAAAAG 
GACCAGATTG AGCAGACTGC CTTCCAGATC ATCTCCCAGG TGATCTTGGA AGCGACTGAA 
GAGATACGGG CCACCACAGT GGGCAAGACT GTGGCACAAG TGCATCCAAC CCCGGGCACT 
CAGCCTCAGG GGCAGGAGGA GAGCTGTGTC CCAGCCAGCC AGGAAACTAG CTTGGGACAA 
GAAATCCCAG ATCCTGCTTC CACCAGAACA GGTGCCACTG CCAGCCCTTC AGCAGGAGCC 
CCACCACCAA AGACCTATGT GAGCTGTCTC AGCAGTCCTC TGTCAGGCCC CACCAAGGAC 
CAGAAGCCAA AGAACTCTGC ACACCACATC TCCCTGGCTC CCTGCCCACC GCCAGTTACC 
CCCCAGAGGC AGTCTCTGGA TGGGGCAAGT AACCCGAGAG GTGATGACAC CTTTGTCACC 
TGTACGTCCA ACAACAGCCA GAGTGTCCTT TCAGTTACCT CCTTGGGGCT GTGCTCAGAC 
CCTGTCAGTA CTTCGAGGCT TGAAGACTCT TGCACAGAGA CCATCTCAAG CTCCGGAGAC 
AAAGCTGTCA CCCCACCACT GCCAGACAGT ACTGAGCCCT TCAGCAATGG GGTGCTGAAG 
GAGGAGTTGT CAGACTTGGG GACCGAGGAT GGATGGACCA TGGATACAGA AGCAGATCAT 
TCAGGAGGTT CTGACGGGAA CAGTATGGAT TCAGTGGATA GCTGTTGTGG GCTTACGAAA 
CCTGATAGCC CCCAGACTGT CCAGGCAGGC TCCAACCCTA AGAAGGTTGA CCTCATCATC 
TGGGAGATCG AGGTGCCAAA GCATTTAGTT GGTCGACTGA TTGGCAAGCA GGGACGGTAC 
GTGAGTTTTC TGAAGCAGAC GTCTGGTGCC AAGATCTACA TCTCCACCCT GCCTTACACA 
CAGAACATCC AGATCTGCCA CATAGAAGGC TCTCAGCATC ATGTGGACAA AGCTCTGAAC 
TTGATTGGGA AGAAGTTTAA GGAGCTGAAC CTCACCAATA TCTATGCACC ACCGCTGCCT 
TCTCTGGCAC TGCCTTCCCT GCCGATGACA TCGTGGCTCA TGCTGCCTGA TGGTATCACT 
GTGGAAGTCA TCGTGGTCAA CCAGGTCAAT GCTGGGCACC TATTTGTACA GCAACACACA 
CACCCCACCT TCCACGCACT GCGCAGCCTG GACCAGCAGA TGTACCTCTG TTACTCTCAG 
CCTGGAATCC CCACCTTGCC CACCCCAGTG GAAATAACGG TTATCTGCGC TGCCCCTGGT 
GCGGACGGGG CCTGGTGGCG AGCCCAAGTA GTGGCCTCCT ATGAGGAGAC CAATGAGGTG 
GAGATTCGCT ACGTGGACTA TGGTGGATAT AAGAGGGTGA AAGTCGACGT GCTCCGGCAA 
ATTAGGTCTG ACTTTGTGAC CCTGCCATTC CAAGGAGCAG AAGTCCTTCT GGACAGTGTG 
GTTCCGCTGT CAGATGATGA TCACTTTTCA CCCGAGGCAG ACGCAGCCAT GAGTGAGATG 
ACAGGCAATA CAGCACTGCT GGCCCAGGTG ACAAGCTACA GTGCGACTGG CCTTCCTCTG 
ATTCAGCTAT GGAGTGTGGT TGGAGATGAA GTGGTGTTGA TAAACCGGTC GCTGGTGGAG 
CGGGGCCTGG CACAGTGGGT AGACAGCTGC TATGCCAGCC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446117.1
CDS	1..2565
Translation	MAIQFRSLFPLALPGMLALLGWWWFFSRKKDRLSSNGKQVGTLKVGPAIEDRLPTEEACPGVLSVTPSVTQPPGKEEQRSMDRPLSDPPALPRTRQVRRRSESSGNLPSIVDTRLQAGQCSDENSKVVLSLMGDEAKSIPLGRPLFPKDLSFPYEAVEGCKQESALGRTPGRGWLSQCAASGENARETGGAEGTGDAVLGESVLEEGLLPQECVSEVEKSEFPILAPGGGGGEKVRSGPPQVDELLKKEEYIVGKLPSSFVGPVHSELVKDEGALVPQVKGSQDRSLARELDKDKTLPEKDQIEQTAFQIISQVILEATEEIRATTVGKTVAQVHPTPGTQPQGQEESCVPASQETSLGQEIPDPASTRTGATASPSAGAPPPKTYVSCLSSPLSGPTKDQKPKNSAHHISLAPCPPPVTPQRQSLDGASNPRGDDTFVTCTSNNSQSVLSVTSLGLCSDPVSTSRLEDSCTETISSSGDKAVTPPLPDSTEPFSNGVLKEELSDLGTEDGWTMDTEADHSGGSDGNSMDSVDSCCGLTKPDSPQTVQAGSNPKKVDLIIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQNIQICHIEGSQHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADGAWWRAQVVASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVVPLSDDDHFSPEADAAMSEMTGNTALLAQVTSYSATGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSCYASL

Target ORF information:

RefSeq Version	NM_053665.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus A-kinase anchoring protein 1 (Akap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053665.1

ORF Insert Sequence:

ATGGCAATCC AGTTCCGCTC ACTCTTCCCC TTGGCCTTGC CTGGAATGCT AGCACTCCTT 
GGCTGGTGGT GGTTTTTCTC TCGTAAAAAA GATCGGCTCA GCAGCAATGG CAAGCAGGTG 
GGGACATTGA AGGTTGGCCC TGCCATCGAG GACCGACTGC CCACTGAAGA GGCCTGTCCT 
GGAGTCCTGT CTGTGACCCC CAGTGTCACA CAGCCTCCTG GAAAGGAAGA GCAGCGCTCT 
ATGGACAGGC CTCTTTCAGA TCCCCCAGCC TTGCCAAGGA CCCGTCAAGT TCGCCGAAGA 
TCAGAGTCCT CAGGCAACCT CCCCAGCATT GTAGACACAA GGTTGCAGGC AGGACAGTGC 
AGCGATGAGA ATTCAAAAGT GGTACTCTCC CTGATGGGGG ACGAAGCCAA ATCTATTCCT 
CTTGGACGTC CGCTTTTCCC AAAGGATCTG TCCTTCCCCT ATGAAGCAGT GGAAGGGTGT 
AAGCAAGAGT CTGCCCTGGG CAGAACTCCT GGGAGAGGCT GGCTAAGCCA GTGTGCAGCC 
TCTGGAGAGA ACGCGAGAGA GACGGGTGGG GCGGAAGGGA CTGGAGATGC TGTGTTGGGG 
GAAAGTGTAC TTGAGGAAGG CCTATTGCCC CAGGAGTGTG TTTCAGAAGT AGAGAAGAGT 
GAGTTTCCGA TCCTAGCTCC CGGGGGAGGC GGGGGAGAGA AGGTGAGAAG TGGCCCACCA 
CAGGTAGATG AGCTTTTAAA GAAGGAAGAA TATATTGTTG GGAAGTTGCC AAGTAGCTTT 
GTGGGGCCAG TTCACTCGGA GCTGGTTAAG GATGAGGGTG CGCTGGTACC CCAAGTCAAA 
GGTAGCCAGG ACAGAAGCCT GGCTAGAGAA CTGGACAAAG ACAAGACCTT GCCTGAAAAG 
GACCAGATTG AGCAGACTGC CTTCCAGATC ATCTCCCAGG TGATCTTGGA AGCGACTGAA 
GAGATACGGG CCACCACAGT GGGCAAGACT GTGGCACAAG TGCATCCAAC CCCGGGCACT 
CAGCCTCAGG GGCAGGAGGA GAGCTGTGTC CCAGCCAGCC AGGAAACTAG CTTGGGACAA 
GAAATCCCAG ATCCTGCTTC CACCAGAACA GGTGCCACTG CCAGCCCTTC AGCAGGAGCC 
CCACCACCAA AGACCTATGT GAGCTGTCTC AGCAGTCCTC TGTCAGGCCC CACCAAGGAC 
CAGAAGCCAA AGAACTCTGC ACACCACATC TCCCTGGCTC CCTGCCCACC GCCAGTTACC 
CCCCAGAGGC AGTCTCTGGA TGGGGCAAGT AACCCGAGAG GTGATGACAC CTTTGTCACC 
TGTACGTCCA ACAACAGCCA GAGTGTCCTT TCAGTTACCT CCTTGGGGCT GTGCTCAGAC 
CCTGTCAGTA CTTCGAGGCT TGAAGACTCT TGCACAGAGA CCATCTCAAG CTCCGGAGAC 
AAAGCTGTCA CCCCACCACT GCCAGACAGT ACTGAGCCCT TCAGCAATGG GGTGCTGAAG 
GAGGAGTTGT CAGACTTGGG GACCGAGGAT GGATGGACCA TGGATACAGA AGCAGATCAT 
TCAGGAGGTT CTGACGGGAA CAGTATGGAT TCAGTGGATA GCTGTTGTGG GCTTACGAAA 
CCTGATAGCC CCCAGACTGT CCAGGCAGGC TCCAACCCTA AGAAGGTTGA CCTCATCATC 
TGGGAGATCG AGGTGCCAAA GCATTTAGTT GGTCGACTGA TTGGCAAGCA GGGACGGTAC 
GTGAGTTTTC TGAAGCAGAC GTCTGGTGCC AAGATCTACA TCTCCACCCT GCCTTACACA 
CAGAACATCC AGATCTGCCA CATAGAAGGC TCTCAGCATC ATGTGGACAA AGCTCTGAAC 
TTGATTGGGA AGAAGTTTAA GGAGCTGAAC CTCACCAATA TCTATGCACC ACCGCTGCCT 
TCTCTGGCAC TGCCTTCCCT GCCGATGACA TCGTGGCTCA TGCTGCCTGA TGGTATCACT 
GTGGAAGTCA TCGTGGTCAA CCAGGTCAAT GCTGGGCACC TATTTGTACA GCAACACACA 
CACCCCACCT TCCACGCACT GCGCAGCCTG GACCAGCAGA TGTACCTCTG TTACTCTCAG 
CCTGGAATCC CCACCTTGCC CACCCCAGTG GAAATAACGG TTATCTGCGC TGCCCCTGGT 
GCGGACGGGG CCTGGTGGCG AGCCCAAGTA GTGGCCTCCT ATGAGGAGAC CAATGAGGTG 
GAGATTCGCT ACGTGGACTA TGGTGGATAT AAGAGGGTGA AAGTCGACGT GCTCCGGCAA 
ATTAGGTCTG ACTTTGTGAC CCTGCCATTC CAAGGAGCAG AAGTCCTTCT GGACAGTGTG 
GTTCCGCTGT CAGATGATGA TCACTTTTCA CCCGAGGCAG ACGCAGCCAT GAGTGAGATG 
ACAGGCAATA CAGCACTGCT GGCCCAGGTG ACAAGCTACA GTGCGACTGG CCTTCCTCTG 
ATTCAGCTAT GGAGTGTGGT TGGAGATGAA GTGGTGTTGA TAAACCGGTC GCTGGTGGAG 
CGGGGCCTGG CACAGTGGGT AGACAGCTGC TATGCCAGCC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akap1 ( XM_006247068.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247130.1 Rattus norvegicus A-kinase anchoring protein 1 (Akap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33547
Clone ID Related Accession (Same CDS sequence)	XM_006247068.1 , XM_006247067.3 , XM_008767930.1
Accession Version	XM_006247068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATCC AGTTCCGTTC ACTCTTCCCC TTGGCCTTGC CTGGAATGCT AGCACTCCTT 
GGCTGGTGGT GGTTTTTCTC TCGTAAAAAA GATCGGCTCA GCAGCAATGG CAAGCAGGTG 
GGGACATTGA AGGTTGGCCC TGCCATCGAG GACCGACTGC CCACTGAAGA GGCCTGTCCT 
GGAGTCCTGT CTGTGACCCC CAGTGTCACA CAGCCTCCTG GAAAGGAAGA GCAGCGCTCT 
ATGGACAGGC CTCTTACAGA TCCCCCAGCC TTGCCAAGGA CCCGTCAAGT TCGCCGAAGA 
TCAGAGTCCT CAGGCAACCT CCCCAGCATT GTAGACACAA GGTTGCAGGC AGGACAGTGC 
AGAGATGAGA ATTCAAAAGT GGTACTCTCC CTGATGGGGG ACGAAGCCAA ATCTATTCCT 
CTTGGACGTC CGCTTTTCCC AAAGGATCTG TCCTTCCCCT ATGAAGCAGT GGAAGGGTGT 
AAGCAAGAGT CTGCCCTGGG CAGAACTCCT GGGAGAGGCT GGCTAAGCCA GTGTGCAGCC 
TCTGGAGAGA ACGCGAGAGA GACGGGTGGG GCGGAAGGGA CTGGAGATGC TGTGTTGGGG 
GAAAGTGTAC TTGAGGAAGG CCTATTGCCC CAGGAGTGTG TTTCAGAAGT AGAGAAGAGT 
GAGTTTCCGA TCCTAGCTCC CGGGGGAGGC GGGGGAGAGA AGGTGAGAAG TGGCCCACCA 
CAGGTAGATG AGCTTTTAAA GAAGGAAGAA TATATTGTTG GGAAGTTGCC AAGTAGCTTT 
GTGGGGCCAG TTCACTCGGA GCTGGTTAAG GATGAGGGTG CGCTGGTACC CCAAGTCAAA 
GGTAGCCAGG ACAGAAGCCT GGCTAGAGAA CTGGACAAAG ACAAGACCTT GCCTGAAAAG 
GACCAGATTG AGCAGACTGC CTTCCAGATC ATCTCCCAGG TGATCTTGGA AGCGACTGAA 
GAGATACGGG CCACCACAGT GGGCAAGACT GTGGCACAAG TGCATCCAAC CCCGGGCACT 
CAGCCTCAGG GGCAGGAGGA GAGCTGTGTC CCAGCCAGCC AGGAAACTAG CTTGGGACAA 
GAAATCCCAG ATCCTGCTTC CACCAGAACA GGTGCCACTG CCAGCCCTTC AGCAGGAGCC 
CCACCACCAA AGACCTATGT GAGCTGTCTC AGCAGTCCTC TGTCAGGCCC CACCAAGGAC 
CAGAAGCCAA AGAACTCTGC ACACCACATC TCCCTGGCTC CCTGCCCACC GCCAGTTACC 
CCCCAGAGGC AGTCTCTGGA TGGGGCAAGT AACCCGAGAG GTGATGACAC CTTTGTCACC 
TGTACGTCCA ACAACAGCCA GAGTGTCCTT TCAGTTACCT CCTTGGGGCT GTGCTCAGAC 
CCTGTCAGTA CTTCGAGGCT TGAAGACTCT TGCACAGAGA CCATCTCAAG CTCCGGAGAC 
AAAGCTGTCA CCCCACCACT GCCAGACAGT ACTGAGCCCT TCAGCAATGG GGTGCTGAAG 
GAGGAGTTGT CAGACTTGGG GACCGAGGAT GGATGGACCA TGGATACAGA AGCAGATCAT 
TCAGGAGGTT CTGACGGGAA CAGTATGGAT TCAGTGGATA GCTGTTGTGG GCTTACGAAA 
CCTGATAGCC CCCAGACTGT CCAGGCAGGC TCCAACCCTA AGAAGGTTGA CCTCATCATC 
TGGGAGATCG AGGTGCCAAA GCATTTAGTT GGTCGACTGA TTGGCAAGCA GGGACGGTAC 
GTGAGTTTTC TGAAGCAGAC GTCTGGTGCC AAGATCTACA TCTCCACCCT GCCTTACACA 
CAGAACATCC AGATCTGCCA CATAGAAGGC TCTCAGCATC ATGTGGACAA AGCTCTGAAC 
TTGATTGGGA AGAAGTTTAA GGAGCTGAAC CTCACCAATA TCTATGCACC ACCGCTGCCT 
TCTCTGGCAC TGCCTTCCCT GCCGATGACA TCGTGGCTCA TGCTGCCTGA TGGTATCACT 
GTGGAAGTCA TCGTGGTCAA CCAGGTCAAT GCTGGGCACC TATTTGTACA GCAACACACA 
CACCCCACCT TCCACGCACT GCGCAGCCTG GACCAGCAGA TGTACCTCTG TTACTCTCAG 
CCTGGAATCC CCACCTTGCC CACCCCAGTG GAAATAACGG TTATCTGCGC TGCCCCTGGT 
GCGGACGGGG CCTGGTGGCG AGCCCAAGTA GTGGCCTCCT ATGAGGAGAC CAATGAGGTG 
GAGATTCGCT ACGTGGACTA TGGTGGATAT AAGAGGGTGA AAGTCGACGT GCTCCGGCAA 
ATTAGGTCTG ACTTTGTGAC CCTGCCATTC CAAGGAGCAG AAGTCCTTCT GGACAGTGTG 
GTTCCGCTGT CAGATGATGA TCACTTTTCA CCCGAGGCAG ACGCAGCCAT GAGTGAGATG 
ACAGGCAATA CAGCACTGCT GGCCCAGGTG ACAAGCTACA GTGCAACTGG CCTTCCTCTG 
ATTCAGCTAT GGAGTGTGGT TGGAGATGAA GTGGTGTTGA TAAACCGGTC GCTGGTGGAG 
CGGGGCCTGG CACAGTGGGT AGACAGCTGC TATGCCAGCC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247130.1
CDS	170..2734
Translation	MAIQFRSLFPLALPGMLALLGWWWFFSRKKDRLSSNGKQVGTLKVGPAIEDRLPTEEACPGVLSVTPSVTQPPGKEEQRSMDRPLTDPPALPRTRQVRRRSESSGNLPSIVDTRLQAGQCRDENSKVVLSLMGDEAKSIPLGRPLFPKDLSFPYEAVEGCKQESALGRTPGRGWLSQCAASGENARETGGAEGTGDAVLGESVLEEGLLPQECVSEVEKSEFPILAPGGGGGEKVRSGPPQVDELLKKEEYIVGKLPSSFVGPVHSELVKDEGALVPQVKGSQDRSLARELDKDKTLPEKDQIEQTAFQIISQVILEATEEIRATTVGKTVAQVHPTPGTQPQGQEESCVPASQETSLGQEIPDPASTRTGATASPSAGAPPPKTYVSCLSSPLSGPTKDQKPKNSAHHISLAPCPPPVTPQRQSLDGASNPRGDDTFVTCTSNNSQSVLSVTSLGLCSDPVSTSRLEDSCTETISSSGDKAVTPPLPDSTEPFSNGVLKEELSDLGTEDGWTMDTEADHSGGSDGNSMDSVDSCCGLTKPDSPQTVQAGSNPKKVDLIIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQNIQICHIEGSQHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADGAWWRAQVVASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVVPLSDDDHFSPEADAAMSEMTGNTALLAQVTSYSATGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSCYASL

Target ORF information:

RefSeq Version	XM_006247068.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus A-kinase anchoring protein 1 (Akap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247068.1

ORF Insert Sequence:

ATGGCAATCC AGTTCCGTTC ACTCTTCCCC TTGGCCTTGC CTGGAATGCT AGCACTCCTT 
GGCTGGTGGT GGTTTTTCTC TCGTAAAAAA GATCGGCTCA GCAGCAATGG CAAGCAGGTG 
GGGACATTGA AGGTTGGCCC TGCCATCGAG GACCGACTGC CCACTGAAGA GGCCTGTCCT 
GGAGTCCTGT CTGTGACCCC CAGTGTCACA CAGCCTCCTG GAAAGGAAGA GCAGCGCTCT 
ATGGACAGGC CTCTTACAGA TCCCCCAGCC TTGCCAAGGA CCCGTCAAGT TCGCCGAAGA 
TCAGAGTCCT CAGGCAACCT CCCCAGCATT GTAGACACAA GGTTGCAGGC AGGACAGTGC 
AGAGATGAGA ATTCAAAAGT GGTACTCTCC CTGATGGGGG ACGAAGCCAA ATCTATTCCT 
CTTGGACGTC CGCTTTTCCC AAAGGATCTG TCCTTCCCCT ATGAAGCAGT GGAAGGGTGT 
AAGCAAGAGT CTGCCCTGGG CAGAACTCCT GGGAGAGGCT GGCTAAGCCA GTGTGCAGCC 
TCTGGAGAGA ACGCGAGAGA GACGGGTGGG GCGGAAGGGA CTGGAGATGC TGTGTTGGGG 
GAAAGTGTAC TTGAGGAAGG CCTATTGCCC CAGGAGTGTG TTTCAGAAGT AGAGAAGAGT 
GAGTTTCCGA TCCTAGCTCC CGGGGGAGGC GGGGGAGAGA AGGTGAGAAG TGGCCCACCA 
CAGGTAGATG AGCTTTTAAA GAAGGAAGAA TATATTGTTG GGAAGTTGCC AAGTAGCTTT 
GTGGGGCCAG TTCACTCGGA GCTGGTTAAG GATGAGGGTG CGCTGGTACC CCAAGTCAAA 
GGTAGCCAGG ACAGAAGCCT GGCTAGAGAA CTGGACAAAG ACAAGACCTT GCCTGAAAAG 
GACCAGATTG AGCAGACTGC CTTCCAGATC ATCTCCCAGG TGATCTTGGA AGCGACTGAA 
GAGATACGGG CCACCACAGT GGGCAAGACT GTGGCACAAG TGCATCCAAC CCCGGGCACT 
CAGCCTCAGG GGCAGGAGGA GAGCTGTGTC CCAGCCAGCC AGGAAACTAG CTTGGGACAA 
GAAATCCCAG ATCCTGCTTC CACCAGAACA GGTGCCACTG CCAGCCCTTC AGCAGGAGCC 
CCACCACCAA AGACCTATGT GAGCTGTCTC AGCAGTCCTC TGTCAGGCCC CACCAAGGAC 
CAGAAGCCAA AGAACTCTGC ACACCACATC TCCCTGGCTC CCTGCCCACC GCCAGTTACC 
CCCCAGAGGC AGTCTCTGGA TGGGGCAAGT AACCCGAGAG GTGATGACAC CTTTGTCACC 
TGTACGTCCA ACAACAGCCA GAGTGTCCTT TCAGTTACCT CCTTGGGGCT GTGCTCAGAC 
CCTGTCAGTA CTTCGAGGCT TGAAGACTCT TGCACAGAGA CCATCTCAAG CTCCGGAGAC 
AAAGCTGTCA CCCCACCACT GCCAGACAGT ACTGAGCCCT TCAGCAATGG GGTGCTGAAG 
GAGGAGTTGT CAGACTTGGG GACCGAGGAT GGATGGACCA TGGATACAGA AGCAGATCAT 
TCAGGAGGTT CTGACGGGAA CAGTATGGAT TCAGTGGATA GCTGTTGTGG GCTTACGAAA 
CCTGATAGCC CCCAGACTGT CCAGGCAGGC TCCAACCCTA AGAAGGTTGA CCTCATCATC 
TGGGAGATCG AGGTGCCAAA GCATTTAGTT GGTCGACTGA TTGGCAAGCA GGGACGGTAC 
GTGAGTTTTC TGAAGCAGAC GTCTGGTGCC AAGATCTACA TCTCCACCCT GCCTTACACA 
CAGAACATCC AGATCTGCCA CATAGAAGGC TCTCAGCATC ATGTGGACAA AGCTCTGAAC 
TTGATTGGGA AGAAGTTTAA GGAGCTGAAC CTCACCAATA TCTATGCACC ACCGCTGCCT 
TCTCTGGCAC TGCCTTCCCT GCCGATGACA TCGTGGCTCA TGCTGCCTGA TGGTATCACT 
GTGGAAGTCA TCGTGGTCAA CCAGGTCAAT GCTGGGCACC TATTTGTACA GCAACACACA 
CACCCCACCT TCCACGCACT GCGCAGCCTG GACCAGCAGA TGTACCTCTG TTACTCTCAG 
CCTGGAATCC CCACCTTGCC CACCCCAGTG GAAATAACGG TTATCTGCGC TGCCCCTGGT 
GCGGACGGGG CCTGGTGGCG AGCCCAAGTA GTGGCCTCCT ATGAGGAGAC CAATGAGGTG 
GAGATTCGCT ACGTGGACTA TGGTGGATAT AAGAGGGTGA AAGTCGACGT GCTCCGGCAA 
ATTAGGTCTG ACTTTGTGAC CCTGCCATTC CAAGGAGCAG AAGTCCTTCT GGACAGTGTG 
GTTCCGCTGT CAGATGATGA TCACTTTTCA CCCGAGGCAG ACGCAGCCAT GAGTGAGATG 
ACAGGCAATA CAGCACTGCT GGCCCAGGTG ACAAGCTACA GTGCAACTGG CCTTCCTCTG 
ATTCAGCTAT GGAGTGTGGT TGGAGATGAA GTGGTGTTGA TAAACCGGTC GCTGGTGGAG 
CGGGGCCTGG CACAGTGGGT AGACAGCTGC TATGCCAGCC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akap1 ( XM_006247067.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247129.1 Rattus norvegicus A-kinase anchoring protein 1 (Akap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa33547
Clone ID Related Accession (Same CDS sequence)	XM_006247068.1 , XM_006247067.3 , XM_008767930.1
Accession Version	XM_006247067.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247067.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATCC AGTTCCGTTC ACTCTTCCCC TTGGCCTTGC CTGGAATGCT AGCACTCCTT 
GGCTGGTGGT GGTTTTTCTC TCGTAAAAAA GATCGGCTCA GCAGCAATGG CAAGCAGGTG 
GGGACATTGA AGGTTGGCCC TGCCATCGAG GACCGACTGC CCACTGAAGA GGCCTGTCCT 
GGAGTCCTGT CTGTGACCCC CAGTGTCACA CAGCCTCCTG GAAAGGAAGA GCAGCGCTCT 
ATGGACAGGC CTCTTACAGA TCCCCCAGCC TTGCCAAGGA CCCGTCAAGT TCGCCGAAGA 
TCAGAGTCCT CAGGCAACCT CCCCAGCATT GTAGACACAA GGTTGCAGGC AGGACAGTGC 
AGAGATGAGA ATTCAAAAGT GGTACTCTCC CTGATGGGGG ACGAAGCCAA ATCTATTCCT 
CTTGGACGTC CGCTTTTCCC AAAGGATCTG TCCTTCCCCT ATGAAGCAGT GGAAGGGTGT 
AAGCAAGAGT CTGCCCTGGG CAGAACTCCT GGGAGAGGCT GGCTAAGCCA GTGTGCAGCC 
TCTGGAGAGA ACGCGAGAGA GACGGGTGGG GCGGAAGGGA CTGGAGATGC TGTGTTGGGG 
GAAAGTGTAC TTGAGGAAGG CCTATTGCCC CAGGAGTGTG TTTCAGAAGT AGAGAAGAGT 
GAGTTTCCGA TCCTAGCTCC CGGGGGAGGC GGGGGAGAGA AGGTGAGAAG TGGCCCACCA 
CAGGTAGATG AGCTTTTAAA GAAGGAAGAA TATATTGTTG GGAAGTTGCC AAGTAGCTTT 
GTGGGGCCAG TTCACTCGGA GCTGGTTAAG GATGAGGGTG CGCTGGTACC CCAAGTCAAA 
GGTAGCCAGG ACAGAAGCCT GGCTAGAGAA CTGGACAAAG ACAAGACCTT GCCTGAAAAG 
GACCAGATTG AGCAGACTGC CTTCCAGATC ATCTCCCAGG TGATCTTGGA AGCGACTGAA 
GAGATACGGG CCACCACAGT GGGCAAGACT GTGGCACAAG TGCATCCAAC CCCGGGCACT 
CAGCCTCAGG GGCAGGAGGA GAGCTGTGTC CCAGCCAGCC AGGAAACTAG CTTGGGACAA 
GAAATCCCAG ATCCTGCTTC CACCAGAACA GGTGCCACTG CCAGCCCTTC AGCAGGAGCC 
CCACCACCAA AGACCTATGT GAGCTGTCTC AGCAGTCCTC TGTCAGGCCC CACCAAGGAC 
CAGAAGCCAA AGAACTCTGC ACACCACATC TCCCTGGCTC CCTGCCCACC GCCAGTTACC 
CCCCAGAGGC AGTCTCTGGA TGGGGCAAGT AACCCGAGAG GTGATGACAC CTTTGTCACC 
TGTACGTCCA ACAACAGCCA GAGTGTCCTT TCAGTTACCT CCTTGGGGCT GTGCTCAGAC 
CCTGTCAGTA CTTCGAGGCT TGAAGACTCT TGCACAGAGA CCATCTCAAG CTCCGGAGAC 
AAAGCTGTCA CCCCACCACT GCCAGACAGT ACTGAGCCCT TCAGCAATGG GGTGCTGAAG 
GAGGAGTTGT CAGACTTGGG GACCGAGGAT GGATGGACCA TGGATACAGA AGCAGATCAT 
TCAGGAGGTT CTGACGGGAA CAGTATGGAT TCAGTGGATA GCTGTTGTGG GCTTACGAAA 
CCTGATAGCC CCCAGACTGT CCAGGCAGGC TCCAACCCTA AGAAGGTTGA CCTCATCATC 
TGGGAGATCG AGGTGCCAAA GCATTTAGTT GGTCGACTGA TTGGCAAGCA GGGACGGTAC 
GTGAGTTTTC TGAAGCAGAC GTCTGGTGCC AAGATCTACA TCTCCACCCT GCCTTACACA 
CAGAACATCC AGATCTGCCA CATAGAAGGC TCTCAGCATC ATGTGGACAA AGCTCTGAAC 
TTGATTGGGA AGAAGTTTAA GGAGCTGAAC CTCACCAATA TCTATGCACC ACCGCTGCCT 
TCTCTGGCAC TGCCTTCCCT GCCGATGACA TCGTGGCTCA TGCTGCCTGA TGGTATCACT 
GTGGAAGTCA TCGTGGTCAA CCAGGTCAAT GCTGGGCACC TATTTGTACA GCAACACACA 
CACCCCACCT TCCACGCACT GCGCAGCCTG GACCAGCAGA TGTACCTCTG TTACTCTCAG 
CCTGGAATCC CCACCTTGCC CACCCCAGTG GAAATAACGG TTATCTGCGC TGCCCCTGGT 
GCGGACGGGG CCTGGTGGCG AGCCCAAGTA GTGGCCTCCT ATGAGGAGAC CAATGAGGTG 
GAGATTCGCT ACGTGGACTA TGGTGGATAT AAGAGGGTGA AAGTCGACGT GCTCCGGCAA 
ATTAGGTCTG ACTTTGTGAC CCTGCCATTC CAAGGAGCAG AAGTCCTTCT GGACAGTGTG 
GTTCCGCTGT CAGATGATGA TCACTTTTCA CCCGAGGCAG ACGCAGCCAT GAGTGAGATG 
ACAGGCAATA CAGCACTGCT GGCCCAGGTG ACAAGCTACA GTGCAACTGG CCTTCCTCTG 
ATTCAGCTAT GGAGTGTGGT TGGAGATGAA GTGGTGTTGA TAAACCGGTC GCTGGTGGAG 
CGGGGCCTGG CACAGTGGGT AGACAGCTGC TATGCCAGCC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247129.1
CDS	168..2732
Translation	MAIQFRSLFPLALPGMLALLGWWWFFSRKKDRLSSNGKQVGTLKVGPAIEDRLPTEEACPGVLSVTPSVTQPPGKEEQRSMDRPLTDPPALPRTRQVRRRSESSGNLPSIVDTRLQAGQCRDENSKVVLSLMGDEAKSIPLGRPLFPKDLSFPYEAVEGCKQESALGRTPGRGWLSQCAASGENARETGGAEGTGDAVLGESVLEEGLLPQECVSEVEKSEFPILAPGGGGGEKVRSGPPQVDELLKKEEYIVGKLPSSFVGPVHSELVKDEGALVPQVKGSQDRSLARELDKDKTLPEKDQIEQTAFQIISQVILEATEEIRATTVGKTVAQVHPTPGTQPQGQEESCVPASQETSLGQEIPDPASTRTGATASPSAGAPPPKTYVSCLSSPLSGPTKDQKPKNSAHHISLAPCPPPVTPQRQSLDGASNPRGDDTFVTCTSNNSQSVLSVTSLGLCSDPVSTSRLEDSCTETISSSGDKAVTPPLPDSTEPFSNGVLKEELSDLGTEDGWTMDTEADHSGGSDGNSMDSVDSCCGLTKPDSPQTVQAGSNPKKVDLIIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQNIQICHIEGSQHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADGAWWRAQVVASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVVPLSDDDHFSPEADAAMSEMTGNTALLAQVTSYSATGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSCYASL

Target ORF information:

RefSeq Version	XM_006247067.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus A-kinase anchoring protein 1 (Akap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247067.3

ORF Insert Sequence:

ATGGCAATCC AGTTCCGTTC ACTCTTCCCC TTGGCCTTGC CTGGAATGCT AGCACTCCTT 
GGCTGGTGGT GGTTTTTCTC TCGTAAAAAA GATCGGCTCA GCAGCAATGG CAAGCAGGTG 
GGGACATTGA AGGTTGGCCC TGCCATCGAG GACCGACTGC CCACTGAAGA GGCCTGTCCT 
GGAGTCCTGT CTGTGACCCC CAGTGTCACA CAGCCTCCTG GAAAGGAAGA GCAGCGCTCT 
ATGGACAGGC CTCTTACAGA TCCCCCAGCC TTGCCAAGGA CCCGTCAAGT TCGCCGAAGA 
TCAGAGTCCT CAGGCAACCT CCCCAGCATT GTAGACACAA GGTTGCAGGC AGGACAGTGC 
AGAGATGAGA ATTCAAAAGT GGTACTCTCC CTGATGGGGG ACGAAGCCAA ATCTATTCCT 
CTTGGACGTC CGCTTTTCCC AAAGGATCTG TCCTTCCCCT ATGAAGCAGT GGAAGGGTGT 
AAGCAAGAGT CTGCCCTGGG CAGAACTCCT GGGAGAGGCT GGCTAAGCCA GTGTGCAGCC 
TCTGGAGAGA ACGCGAGAGA GACGGGTGGG GCGGAAGGGA CTGGAGATGC TGTGTTGGGG 
GAAAGTGTAC TTGAGGAAGG CCTATTGCCC CAGGAGTGTG TTTCAGAAGT AGAGAAGAGT 
GAGTTTCCGA TCCTAGCTCC CGGGGGAGGC GGGGGAGAGA AGGTGAGAAG TGGCCCACCA 
CAGGTAGATG AGCTTTTAAA GAAGGAAGAA TATATTGTTG GGAAGTTGCC AAGTAGCTTT 
GTGGGGCCAG TTCACTCGGA GCTGGTTAAG GATGAGGGTG CGCTGGTACC CCAAGTCAAA 
GGTAGCCAGG ACAGAAGCCT GGCTAGAGAA CTGGACAAAG ACAAGACCTT GCCTGAAAAG 
GACCAGATTG AGCAGACTGC CTTCCAGATC ATCTCCCAGG TGATCTTGGA AGCGACTGAA 
GAGATACGGG CCACCACAGT GGGCAAGACT GTGGCACAAG TGCATCCAAC CCCGGGCACT 
CAGCCTCAGG GGCAGGAGGA GAGCTGTGTC CCAGCCAGCC AGGAAACTAG CTTGGGACAA 
GAAATCCCAG ATCCTGCTTC CACCAGAACA GGTGCCACTG CCAGCCCTTC AGCAGGAGCC 
CCACCACCAA AGACCTATGT GAGCTGTCTC AGCAGTCCTC TGTCAGGCCC CACCAAGGAC 
CAGAAGCCAA AGAACTCTGC ACACCACATC TCCCTGGCTC CCTGCCCACC GCCAGTTACC 
CCCCAGAGGC AGTCTCTGGA TGGGGCAAGT AACCCGAGAG GTGATGACAC CTTTGTCACC 
TGTACGTCCA ACAACAGCCA GAGTGTCCTT TCAGTTACCT CCTTGGGGCT GTGCTCAGAC 
CCTGTCAGTA CTTCGAGGCT TGAAGACTCT TGCACAGAGA CCATCTCAAG CTCCGGAGAC 
AAAGCTGTCA CCCCACCACT GCCAGACAGT ACTGAGCCCT TCAGCAATGG GGTGCTGAAG 
GAGGAGTTGT CAGACTTGGG GACCGAGGAT GGATGGACCA TGGATACAGA AGCAGATCAT 
TCAGGAGGTT CTGACGGGAA CAGTATGGAT TCAGTGGATA GCTGTTGTGG GCTTACGAAA 
CCTGATAGCC CCCAGACTGT CCAGGCAGGC TCCAACCCTA AGAAGGTTGA CCTCATCATC 
TGGGAGATCG AGGTGCCAAA GCATTTAGTT GGTCGACTGA TTGGCAAGCA GGGACGGTAC 
GTGAGTTTTC TGAAGCAGAC GTCTGGTGCC AAGATCTACA TCTCCACCCT GCCTTACACA 
CAGAACATCC AGATCTGCCA CATAGAAGGC TCTCAGCATC ATGTGGACAA AGCTCTGAAC 
TTGATTGGGA AGAAGTTTAA GGAGCTGAAC CTCACCAATA TCTATGCACC ACCGCTGCCT 
TCTCTGGCAC TGCCTTCCCT GCCGATGACA TCGTGGCTCA TGCTGCCTGA TGGTATCACT 
GTGGAAGTCA TCGTGGTCAA CCAGGTCAAT GCTGGGCACC TATTTGTACA GCAACACACA 
CACCCCACCT TCCACGCACT GCGCAGCCTG GACCAGCAGA TGTACCTCTG TTACTCTCAG 
CCTGGAATCC CCACCTTGCC CACCCCAGTG GAAATAACGG TTATCTGCGC TGCCCCTGGT 
GCGGACGGGG CCTGGTGGCG AGCCCAAGTA GTGGCCTCCT ATGAGGAGAC CAATGAGGTG 
GAGATTCGCT ACGTGGACTA TGGTGGATAT AAGAGGGTGA AAGTCGACGT GCTCCGGCAA 
ATTAGGTCTG ACTTTGTGAC CCTGCCATTC CAAGGAGCAG AAGTCCTTCT GGACAGTGTG 
GTTCCGCTGT CAGATGATGA TCACTTTTCA CCCGAGGCAG ACGCAGCCAT GAGTGAGATG 
ACAGGCAATA CAGCACTGCT GGCCCAGGTG ACAAGCTACA GTGCAACTGG CCTTCCTCTG 
ATTCAGCTAT GGAGTGTGGT TGGAGATGAA GTGGTGTTGA TAAACCGGTC GCTGGTGGAG 
CGGGGCCTGG CACAGTGGGT AGACAGCTGC TATGCCAGCC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akap1 ( XM_008767930.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766152.1 Rattus norvegicus A-kinase anchoring protein 1 (Akap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa33547
Clone ID Related Accession (Same CDS sequence)	XM_006247068.1 , XM_006247067.3 , XM_008767930.1
Accession Version	XM_008767930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATCC AGTTCCGTTC ACTCTTCCCC TTGGCCTTGC CTGGAATGCT AGCACTCCTT 
GGCTGGTGGT GGTTTTTCTC TCGTAAAAAA GATCGGCTCA GCAGCAATGG CAAGCAGGTG 
GGGACATTGA AGGTTGGCCC TGCCATCGAG GACCGACTGC CCACTGAAGA GGCCTGTCCT 
GGAGTCCTGT CTGTGACCCC CAGTGTCACA CAGCCTCCTG GAAAGGAAGA GCAGCGCTCT 
ATGGACAGGC CTCTTACAGA TCCCCCAGCC TTGCCAAGGA CCCGTCAAGT TCGCCGAAGA 
TCAGAGTCCT CAGGCAACCT CCCCAGCATT GTAGACACAA GGTTGCAGGC AGGACAGTGC 
AGAGATGAGA ATTCAAAAGT GGTACTCTCC CTGATGGGGG ACGAAGCCAA ATCTATTCCT 
CTTGGACGTC CGCTTTTCCC AAAGGATCTG TCCTTCCCCT ATGAAGCAGT GGAAGGGTGT 
AAGCAAGAGT CTGCCCTGGG CAGAACTCCT GGGAGAGGCT GGCTAAGCCA GTGTGCAGCC 
TCTGGAGAGA ACGCGAGAGA GACGGGTGGG GCGGAAGGGA CTGGAGATGC TGTGTTGGGG 
GAAAGTGTAC TTGAGGAAGG CCTATTGCCC CAGGAGTGTG TTTCAGAAGT AGAGAAGAGT 
GAGTTTCCGA TCCTAGCTCC CGGGGGAGGC GGGGGAGAGA AGGTGAGAAG TGGCCCACCA 
CAGGTAGATG AGCTTTTAAA GAAGGAAGAA TATATTGTTG GGAAGTTGCC AAGTAGCTTT 
GTGGGGCCAG TTCACTCGGA GCTGGTTAAG GATGAGGGTG CGCTGGTACC CCAAGTCAAA 
GGTAGCCAGG ACAGAAGCCT GGCTAGAGAA CTGGACAAAG ACAAGACCTT GCCTGAAAAG 
GACCAGATTG AGCAGACTGC CTTCCAGATC ATCTCCCAGG TGATCTTGGA AGCGACTGAA 
GAGATACGGG CCACCACAGT GGGCAAGACT GTGGCACAAG TGCATCCAAC CCCGGGCACT 
CAGCCTCAGG GGCAGGAGGA GAGCTGTGTC CCAGCCAGCC AGGAAACTAG CTTGGGACAA 
GAAATCCCAG ATCCTGCTTC CACCAGAACA GGTGCCACTG CCAGCCCTTC AGCAGGAGCC 
CCACCACCAA AGACCTATGT GAGCTGTCTC AGCAGTCCTC TGTCAGGCCC CACCAAGGAC 
CAGAAGCCAA AGAACTCTGC ACACCACATC TCCCTGGCTC CCTGCCCACC GCCAGTTACC 
CCCCAGAGGC AGTCTCTGGA TGGGGCAAGT AACCCGAGAG GTGATGACAC CTTTGTCACC 
TGTACGTCCA ACAACAGCCA GAGTGTCCTT TCAGTTACCT CCTTGGGGCT GTGCTCAGAC 
CCTGTCAGTA CTTCGAGGCT TGAAGACTCT TGCACAGAGA CCATCTCAAG CTCCGGAGAC 
AAAGCTGTCA CCCCACCACT GCCAGACAGT ACTGAGCCCT TCAGCAATGG GGTGCTGAAG 
GAGGAGTTGT CAGACTTGGG GACCGAGGAT GGATGGACCA TGGATACAGA AGCAGATCAT 
TCAGGAGGTT CTGACGGGAA CAGTATGGAT TCAGTGGATA GCTGTTGTGG GCTTACGAAA 
CCTGATAGCC CCCAGACTGT CCAGGCAGGC TCCAACCCTA AGAAGGTTGA CCTCATCATC 
TGGGAGATCG AGGTGCCAAA GCATTTAGTT GGTCGACTGA TTGGCAAGCA GGGACGGTAC 
GTGAGTTTTC TGAAGCAGAC GTCTGGTGCC AAGATCTACA TCTCCACCCT GCCTTACACA 
CAGAACATCC AGATCTGCCA CATAGAAGGC TCTCAGCATC ATGTGGACAA AGCTCTGAAC 
TTGATTGGGA AGAAGTTTAA GGAGCTGAAC CTCACCAATA TCTATGCACC ACCGCTGCCT 
TCTCTGGCAC TGCCTTCCCT GCCGATGACA TCGTGGCTCA TGCTGCCTGA TGGTATCACT 
GTGGAAGTCA TCGTGGTCAA CCAGGTCAAT GCTGGGCACC TATTTGTACA GCAACACACA 
CACCCCACCT TCCACGCACT GCGCAGCCTG GACCAGCAGA TGTACCTCTG TTACTCTCAG 
CCTGGAATCC CCACCTTGCC CACCCCAGTG GAAATAACGG TTATCTGCGC TGCCCCTGGT 
GCGGACGGGG CCTGGTGGCG AGCCCAAGTA GTGGCCTCCT ATGAGGAGAC CAATGAGGTG 
GAGATTCGCT ACGTGGACTA TGGTGGATAT AAGAGGGTGA AAGTCGACGT GCTCCGGCAA 
ATTAGGTCTG ACTTTGTGAC CCTGCCATTC CAAGGAGCAG AAGTCCTTCT GGACAGTGTG 
GTTCCGCTGT CAGATGATGA TCACTTTTCA CCCGAGGCAG ACGCAGCCAT GAGTGAGATG 
ACAGGCAATA CAGCACTGCT GGCCCAGGTG ACAAGCTACA GTGCAACTGG CCTTCCTCTG 
ATTCAGCTAT GGAGTGTGGT TGGAGATGAA GTGGTGTTGA TAAACCGGTC GCTGGTGGAG 
CGGGGCCTGG CACAGTGGGT AGACAGCTGC TATGCCAGCC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766152.1
CDS	229..2793
Translation	MAIQFRSLFPLALPGMLALLGWWWFFSRKKDRLSSNGKQVGTLKVGPAIEDRLPTEEACPGVLSVTPSVTQPPGKEEQRSMDRPLTDPPALPRTRQVRRRSESSGNLPSIVDTRLQAGQCRDENSKVVLSLMGDEAKSIPLGRPLFPKDLSFPYEAVEGCKQESALGRTPGRGWLSQCAASGENARETGGAEGTGDAVLGESVLEEGLLPQECVSEVEKSEFPILAPGGGGGEKVRSGPPQVDELLKKEEYIVGKLPSSFVGPVHSELVKDEGALVPQVKGSQDRSLARELDKDKTLPEKDQIEQTAFQIISQVILEATEEIRATTVGKTVAQVHPTPGTQPQGQEESCVPASQETSLGQEIPDPASTRTGATASPSAGAPPPKTYVSCLSSPLSGPTKDQKPKNSAHHISLAPCPPPVTPQRQSLDGASNPRGDDTFVTCTSNNSQSVLSVTSLGLCSDPVSTSRLEDSCTETISSSGDKAVTPPLPDSTEPFSNGVLKEELSDLGTEDGWTMDTEADHSGGSDGNSMDSVDSCCGLTKPDSPQTVQAGSNPKKVDLIIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQNIQICHIEGSQHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADGAWWRAQVVASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVVPLSDDDHFSPEADAAMSEMTGNTALLAQVTSYSATGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSCYASL

Target ORF information:

RefSeq Version	XM_008767930.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus A-kinase anchoring protein 1 (Akap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767930.1

ORF Insert Sequence:

ATGGCAATCC AGTTCCGTTC ACTCTTCCCC TTGGCCTTGC CTGGAATGCT AGCACTCCTT 
GGCTGGTGGT GGTTTTTCTC TCGTAAAAAA GATCGGCTCA GCAGCAATGG CAAGCAGGTG 
GGGACATTGA AGGTTGGCCC TGCCATCGAG GACCGACTGC CCACTGAAGA GGCCTGTCCT 
GGAGTCCTGT CTGTGACCCC CAGTGTCACA CAGCCTCCTG GAAAGGAAGA GCAGCGCTCT 
ATGGACAGGC CTCTTACAGA TCCCCCAGCC TTGCCAAGGA CCCGTCAAGT TCGCCGAAGA 
TCAGAGTCCT CAGGCAACCT CCCCAGCATT GTAGACACAA GGTTGCAGGC AGGACAGTGC 
AGAGATGAGA ATTCAAAAGT GGTACTCTCC CTGATGGGGG ACGAAGCCAA ATCTATTCCT 
CTTGGACGTC CGCTTTTCCC AAAGGATCTG TCCTTCCCCT ATGAAGCAGT GGAAGGGTGT 
AAGCAAGAGT CTGCCCTGGG CAGAACTCCT GGGAGAGGCT GGCTAAGCCA GTGTGCAGCC 
TCTGGAGAGA ACGCGAGAGA GACGGGTGGG GCGGAAGGGA CTGGAGATGC TGTGTTGGGG 
GAAAGTGTAC TTGAGGAAGG CCTATTGCCC CAGGAGTGTG TTTCAGAAGT AGAGAAGAGT 
GAGTTTCCGA TCCTAGCTCC CGGGGGAGGC GGGGGAGAGA AGGTGAGAAG TGGCCCACCA 
CAGGTAGATG AGCTTTTAAA GAAGGAAGAA TATATTGTTG GGAAGTTGCC AAGTAGCTTT 
GTGGGGCCAG TTCACTCGGA GCTGGTTAAG GATGAGGGTG CGCTGGTACC CCAAGTCAAA 
GGTAGCCAGG ACAGAAGCCT GGCTAGAGAA CTGGACAAAG ACAAGACCTT GCCTGAAAAG 
GACCAGATTG AGCAGACTGC CTTCCAGATC ATCTCCCAGG TGATCTTGGA AGCGACTGAA 
GAGATACGGG CCACCACAGT GGGCAAGACT GTGGCACAAG TGCATCCAAC CCCGGGCACT 
CAGCCTCAGG GGCAGGAGGA GAGCTGTGTC CCAGCCAGCC AGGAAACTAG CTTGGGACAA 
GAAATCCCAG ATCCTGCTTC CACCAGAACA GGTGCCACTG CCAGCCCTTC AGCAGGAGCC 
CCACCACCAA AGACCTATGT GAGCTGTCTC AGCAGTCCTC TGTCAGGCCC CACCAAGGAC 
CAGAAGCCAA AGAACTCTGC ACACCACATC TCCCTGGCTC CCTGCCCACC GCCAGTTACC 
CCCCAGAGGC AGTCTCTGGA TGGGGCAAGT AACCCGAGAG GTGATGACAC CTTTGTCACC 
TGTACGTCCA ACAACAGCCA GAGTGTCCTT TCAGTTACCT CCTTGGGGCT GTGCTCAGAC 
CCTGTCAGTA CTTCGAGGCT TGAAGACTCT TGCACAGAGA CCATCTCAAG CTCCGGAGAC 
AAAGCTGTCA CCCCACCACT GCCAGACAGT ACTGAGCCCT TCAGCAATGG GGTGCTGAAG 
GAGGAGTTGT CAGACTTGGG GACCGAGGAT GGATGGACCA TGGATACAGA AGCAGATCAT 
TCAGGAGGTT CTGACGGGAA CAGTATGGAT TCAGTGGATA GCTGTTGTGG GCTTACGAAA 
CCTGATAGCC CCCAGACTGT CCAGGCAGGC TCCAACCCTA AGAAGGTTGA CCTCATCATC 
TGGGAGATCG AGGTGCCAAA GCATTTAGTT GGTCGACTGA TTGGCAAGCA GGGACGGTAC 
GTGAGTTTTC TGAAGCAGAC GTCTGGTGCC AAGATCTACA TCTCCACCCT GCCTTACACA 
CAGAACATCC AGATCTGCCA CATAGAAGGC TCTCAGCATC ATGTGGACAA AGCTCTGAAC 
TTGATTGGGA AGAAGTTTAA GGAGCTGAAC CTCACCAATA TCTATGCACC ACCGCTGCCT 
TCTCTGGCAC TGCCTTCCCT GCCGATGACA TCGTGGCTCA TGCTGCCTGA TGGTATCACT 
GTGGAAGTCA TCGTGGTCAA CCAGGTCAAT GCTGGGCACC TATTTGTACA GCAACACACA 
CACCCCACCT TCCACGCACT GCGCAGCCTG GACCAGCAGA TGTACCTCTG TTACTCTCAG 
CCTGGAATCC CCACCTTGCC CACCCCAGTG GAAATAACGG TTATCTGCGC TGCCCCTGGT 
GCGGACGGGG CCTGGTGGCG AGCCCAAGTA GTGGCCTCCT ATGAGGAGAC CAATGAGGTG 
GAGATTCGCT ACGTGGACTA TGGTGGATAT AAGAGGGTGA AAGTCGACGT GCTCCGGCAA 
ATTAGGTCTG ACTTTGTGAC CCTGCCATTC CAAGGAGCAG AAGTCCTTCT GGACAGTGTG 
GTTCCGCTGT CAGATGATGA TCACTTTTCA CCCGAGGCAG ACGCAGCCAT GAGTGAGATG 
ACAGGCAATA CAGCACTGCT GGCCCAGGTG ACAAGCTACA GTGCAACTGG CCTTCCTCTG 
ATTCAGCTAT GGAGTGTGGT TGGAGATGAA GTGGTGTTGA TAAACCGGTC GCTGGTGGAG 
CGGGGCCTGG CACAGTGGGT AGACAGCTGC TATGCCAGCC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Mechanism of neuroprotective mitochondrial remodeling by PKA/AKAP1.
PLoS biology9(4)e1000612(2011 Apr)
Merrill RA,Dagda RK,Dickey AS,Cribbs JT,Green SH,Usachev YM,Strack S

AKAP121 downregulation impairs protective cAMP signals, promotes mitochondrial dysfunction, and increases oxidative stress.
Cardiovascular research88(1)101-10(2010 Oct)
Perrino C,Feliciello A,Schiattarella GG,Esposito G,Guerriero R,Zaccaro L,Del Gatto A,Saviano M,Garbi C,Carangi R,Di Lorenzo E,Donato G,Indolfi C,Avvedimento VE,Chiariello M

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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Akap1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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