Gng12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gng12
Entrez Gene ID	114120
Full Name	G protein subunit gamma 12
General protein information	Preferred Names G protein subunit gamma 12 Names guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 guanine nucleotide binding protein (G protein), gamma 12
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q31
Summary	gamma 12 subunit of heterotrimeric G-proteins, which exchange GDP for GTP and activate downstream effectors [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008762970.2	XP_008761192.1	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 isoform X1
XM_006236612.2	XP_006236674.1	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 isoform X1
XM_006236610.2	XP_006236672.1	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 isoform X1
XM_008762971.2	XP_008761193.1	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 isoform X1
XM_008762972.2	XP_008761194.1	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 isoform X1
NM_053661.1	NP_446113.1	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GNG12	NP_777210.1
Rattus norvegicus (Norway rat)	Gng12	NP_446113.1
Danio rerio (zebrafish)	LOC100331581	XP_005168802.1
Homo sapiens (human)	GNG12	NP_061329.3
Mus musculus (house mouse)	Gng12	NP_079554.1
Pan troglodytes (chimpanzee)	GNG12	XP_003339037.1
	GNG12	XP_002801655.1
Gallus gallus (chicken)	GNG12	XP_001234703.2
Canis lupus familiaris (dog)	GNG12	XP_005622119.1
Danio rerio (zebrafish)	gng12a	NP_998477.2
Xenopus tropicalis (tropical clawed frog)	gng12	NP_001096165.1

Gene Ontology
Bibliography

Related articles in PubMed

KIF14 negatively regulates Rap1a-Radil signaling during breast cancer progression.
Ahmed SM, Th?riault BL, Uppalapati M, Chiu CW, Gallie BL, Sidhu SS, Angers S
The Journal of cell biology199(6)951-67(2012 Dec)

Gng12 is a novel negative regulator of LPS-induced inflammation in the microglial cell line BV-2.
Larson KC, Lipko M, Dabrowski M, Draper MP
Inflammation research : official journal of the European Histamine Research Society ... [et al.]59(1)15-22(2010 Jan)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Distribution of heterotrimeric G-protein beta and gamma subunits in the rat brain.
Betty M, Harnish SW, Rhodes KJ, Cockett MI
Neuroscience85(2)475-86(1998 Jul)

GTP-binding protein gamma12 subunit phosphorylation by protein kinase C--identification of the phosphorylation site and factors involved in cultured cells and rat tissues in vivo.
Asano T, Morishita R, Ueda H, Asano M, Kato K
European journal of biochemistry251(1-2)314-9(1998 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gng12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gng12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10237	NM_053661.1 Latest version!	Rattus norvegicus G protein subunit gamma 12 (Gng12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa10237	XM_008762970.2 Latest version!	Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa10237	XM_006236610.2 Latest version!	Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa10237	XM_008762972.2 Latest version!	Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa10237	XM_006236612.2 Latest version!	Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa10237	XM_008762971.2 Latest version!	Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gng12 ( NM_053661.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446113.1 Rattus norvegicus G protein subunit gamma 12 (Gng12), mRNA.

Price & Availability↑

CloneID	ORa10237
Clone ID Related Accession (Same CDS sequence)	XM_008762970.2 , XM_006236612.2 , XM_006236610.2 , XM_008762971.2 , XM_008762972.2 , NM_053661.1
Accession Version	NM_053661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-30
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM031435.1 and BG378509.1. On or before May 12, 2012 this sequence version replaced XM_578287.3, XM_001067408.2. Sequence Note: This RefSeq record was created from transcripts of different strains because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: CF113050.1, BF554406.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGCA AGACAGCGAG CACCAACAGC ACAGCCCAAG CTAGGAGAAC TGTGCAGCAG 
CTGAGATTGG AAGCCTCCAT CGAAAGAATA AAGGTCTCCA AAGCATCTGC AGACCTGATG 
TCATACTGTG AGGAGCATGC CCGGAGCGAC CCTCTGCTGA TGGGCATACC GACCTCAGAA 
AACCCATTCA AGGATAAGAA GACCTGCATC ATCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446113.1
CDS	138..356
Translation	MSSKTASTNSTAQARRTVQQLRLEASIERIKVSKASADLMSYCEEHARSDPLLMGIPTSENPFKDKKTCIIL

Target ORF information:

RefSeq Version	NM_053661.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit gamma 12 (Gng12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053661.1

ORF Insert Sequence:

ATGTCCAGCA AGACAGCGAG CACCAACAGC ACAGCCCAAG CTAGGAGAAC TGTGCAGCAG 
CTGAGATTGG AAGCCTCCAT CGAAAGAATA AAGGTCTCCA AAGCATCTGC AGACCTGATG 
TCATACTGTG AGGAGCATGC CCGGAGCGAC CCTCTGCTGA TGGGCATACC GACCTCAGAA 
AACCCATTCA AGGATAAGAA GACCTGCATC ATCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gng12 ( XM_008762970.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761192.1 Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa10237
Clone ID Related Accession (Same CDS sequence)	XM_008762970.2 , XM_006236612.2 , XM_006236610.2 , XM_008762971.2 , XM_008762972.2 , NM_053661.1
Accession Version	XM_008762970.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762970.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AGACAGCGAG CACCAACAGC ACAGCCCAAG CTAGGAGAAC TGTGCAGCAG 
CTGAGATTGG AAGCCTCCAT CGAAAGAATA AAGGTCTCCA AAGCATCTGC AGACCTGATG 
TCATACTGTG AGGAGCATGC CCGGAGCGAC CCTCTGCTGA TGGGCATACC GACCTCAGAA 
AACCCATTCA AGGATAAGAA GACCTGCATC ATCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761192.1
CDS	571..789
Translation	MSSKTASTNSTAQARRTVQQLRLEASIERIKVSKASADLMSYCEEHARSDPLLMGIPTSENPFKDKKTCIIL

Target ORF information:

RefSeq Version	XM_008762970.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762970.2

ORF Insert Sequence:

ATGTCCAGCA AGACAGCGAG CACCAACAGC ACAGCCCAAG CTAGGAGAAC TGTGCAGCAG 
CTGAGATTGG AAGCCTCCAT CGAAAGAATA AAGGTCTCCA AAGCATCTGC AGACCTGATG 
TCATACTGTG AGGAGCATGC CCGGAGCGAC CCTCTGCTGA TGGGCATACC GACCTCAGAA 
AACCCATTCA AGGATAAGAA GACCTGCATC ATCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gng12 ( XM_006236612.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236674.1 Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa10237
Clone ID Related Accession (Same CDS sequence)	XM_008762970.2 , XM_006236612.2 , XM_006236610.2 , XM_008762971.2 , XM_008762972.2 , NM_053661.1
Accession Version	XM_006236612.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006236612.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AGACAGCGAG CACCAACAGC ACAGCCCAAG CTAGGAGAAC TGTGCAGCAG 
CTGAGATTGG AAGCCTCCAT CGAAAGAATA AAGGTCTCCA AAGCATCTGC AGACCTGATG 
TCATACTGTG AGGAGCATGC CCGGAGCGAC CCTCTGCTGA TGGGCATACC GACCTCAGAA 
AACCCATTCA AGGATAAGAA GACCTGCATC ATCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236674.1
CDS	103..321
Translation	MSSKTASTNSTAQARRTVQQLRLEASIERIKVSKASADLMSYCEEHARSDPLLMGIPTSENPFKDKKTCIIL

Target ORF information:

RefSeq Version	XM_006236612.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236612.2

ORF Insert Sequence:

ATGTCCAGCA AGACAGCGAG CACCAACAGC ACAGCCCAAG CTAGGAGAAC TGTGCAGCAG 
CTGAGATTGG AAGCCTCCAT CGAAAGAATA AAGGTCTCCA AAGCATCTGC AGACCTGATG 
TCATACTGTG AGGAGCATGC CCGGAGCGAC CCTCTGCTGA TGGGCATACC GACCTCAGAA 
AACCCATTCA AGGATAAGAA GACCTGCATC ATCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gng12 ( XM_006236610.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236672.1 Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa10237
Clone ID Related Accession (Same CDS sequence)	XM_008762970.2 , XM_006236612.2 , XM_006236610.2 , XM_008762971.2 , XM_008762972.2 , NM_053661.1
Accession Version	XM_006236610.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006236610.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AGACAGCGAG CACCAACAGC ACAGCCCAAG CTAGGAGAAC TGTGCAGCAG 
CTGAGATTGG AAGCCTCCAT CGAAAGAATA AAGGTCTCCA AAGCATCTGC AGACCTGATG 
TCATACTGTG AGGAGCATGC CCGGAGCGAC CCTCTGCTGA TGGGCATACC GACCTCAGAA 
AACCCATTCA AGGATAAGAA GACCTGCATC ATCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236672.1
CDS	702..920
Translation	MSSKTASTNSTAQARRTVQQLRLEASIERIKVSKASADLMSYCEEHARSDPLLMGIPTSENPFKDKKTCIIL

Target ORF information:

RefSeq Version	XM_006236610.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236610.2

ORF Insert Sequence:

ATGTCCAGCA AGACAGCGAG CACCAACAGC ACAGCCCAAG CTAGGAGAAC TGTGCAGCAG 
CTGAGATTGG AAGCCTCCAT CGAAAGAATA AAGGTCTCCA AAGCATCTGC AGACCTGATG 
TCATACTGTG AGGAGCATGC CCGGAGCGAC CCTCTGCTGA TGGGCATACC GACCTCAGAA 
AACCCATTCA AGGATAAGAA GACCTGCATC ATCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gng12 ( XM_008762971.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761193.1 Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa10237
Clone ID Related Accession (Same CDS sequence)	XM_008762970.2 , XM_006236612.2 , XM_006236610.2 , XM_008762971.2 , XM_008762972.2 , NM_053661.1
Accession Version	XM_008762971.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AGACAGCGAG CACCAACAGC ACAGCCCAAG CTAGGAGAAC TGTGCAGCAG 
CTGAGATTGG AAGCCTCCAT CGAAAGAATA AAGGTCTCCA AAGCATCTGC AGACCTGATG 
TCATACTGTG AGGAGCATGC CCGGAGCGAC CCTCTGCTGA TGGGCATACC GACCTCAGAA 
AACCCATTCA AGGATAAGAA GACCTGCATC ATCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761193.1
CDS	756..974
Translation	MSSKTASTNSTAQARRTVQQLRLEASIERIKVSKASADLMSYCEEHARSDPLLMGIPTSENPFKDKKTCIIL

Target ORF information:

RefSeq Version	XM_008762971.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762971.2

ORF Insert Sequence:

ATGTCCAGCA AGACAGCGAG CACCAACAGC ACAGCCCAAG CTAGGAGAAC TGTGCAGCAG 
CTGAGATTGG AAGCCTCCAT CGAAAGAATA AAGGTCTCCA AAGCATCTGC AGACCTGATG 
TCATACTGTG AGGAGCATGC CCGGAGCGAC CCTCTGCTGA TGGGCATACC GACCTCAGAA 
AACCCATTCA AGGATAAGAA GACCTGCATC ATCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gng12 ( XM_008762972.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761194.1 Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa10237
Clone ID Related Accession (Same CDS sequence)	XM_008762970.2 , XM_006236612.2 , XM_006236610.2 , XM_008762971.2 , XM_008762972.2 , NM_053661.1
Accession Version	XM_008762972.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AGACAGCGAG CACCAACAGC ACAGCCCAAG CTAGGAGAAC TGTGCAGCAG 
CTGAGATTGG AAGCCTCCAT CGAAAGAATA AAGGTCTCCA AAGCATCTGC AGACCTGATG 
TCATACTGTG AGGAGCATGC CCGGAGCGAC CCTCTGCTGA TGGGCATACC GACCTCAGAA 
AACCCATTCA AGGATAAGAA GACCTGCATC ATCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761194.1
CDS	602..820
Translation	MSSKTASTNSTAQARRTVQQLRLEASIERIKVSKASADLMSYCEEHARSDPLLMGIPTSENPFKDKKTCIIL

Target ORF information:

RefSeq Version	XM_008762972.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit gamma 12 (Gng12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762972.2

ORF Insert Sequence:

ATGTCCAGCA AGACAGCGAG CACCAACAGC ACAGCCCAAG CTAGGAGAAC TGTGCAGCAG 
CTGAGATTGG AAGCCTCCAT CGAAAGAATA AAGGTCTCCA AAGCATCTGC AGACCTGATG 
TCATACTGTG AGGAGCATGC CCGGAGCGAC CCTCTGCTGA TGGGCATACC GACCTCAGAA 
AACCCATTCA AGGATAAGAA GACCTGCATC ATCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

KIF14 negatively regulates Rap1a-Radil signaling during breast cancer progression.
The Journal of cell biology199(6)951-67(2012 Dec)
Ahmed SM,Th?riault BL,Uppalapati M,Chiu CW,Gallie BL,Sidhu SS,Angers S

Gng12 is a novel negative regulator of LPS-induced inflammation in the microglial cell line BV-2.
Inflammation research : official journal of the European Histamine Research Society ... [et al.]59(1)15-22(2010 Jan)
Larson KC,Lipko M,Dabrowski M,Draper MP

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Distribution of heterotrimeric G-protein beta and gamma subunits in the rat brain.
Neuroscience85(2)475-86(1998 Jul)
Betty M,Harnish SW,Rhodes KJ,Cockett MI

GTP-binding protein gamma12 subunit phosphorylation by protein kinase C--identification of the phosphorylation site and factors involved in cultured cells and rat tissues in vivo.
European journal of biochemistry251(1-2)314-9(1998 Jan)
Asano T,Morishita R,Ueda H,Asano M,Kato K

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Gng12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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