Nkx2-5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nkx2-5
Entrez Gene ID	114109
Full Name	NK2 homeobox 5
Synonyms	Csx,Nkx2.5
General protein information	Preferred Names NK2 homeobox 5 Names homeobox protein Nkx-2.5 homeobox protein NK-2 homolog E rNKx-2.5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12
Summary	homeobox transcription factor important for cardiac development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053651.1	NP_446103.1	homeobox protein Nkx-2.5

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nkx2-5	NP_032726.1
Homo sapiens (human)	NKX2-5	NP_004378.1
Xenopus tropicalis (tropical clawed frog)	nkx2-5	NP_001116891.1
Bos taurus (cattle)	NKX2-5	NP_001039908.1
Gallus gallus (chicken)	NKX2-5	NP_990495.1
Danio rerio (zebrafish)	nkx2.5	NP_571496.1
Canis lupus familiaris (dog)	NKX2-5	NP_001010959.1
Rattus norvegicus (Norway rat)	Nkx2-5	NP_446103.1
Pan troglodytes (chimpanzee)	NKX2-5	XP_518104.2
	NKX2-5	XP_001096796.1

Gene Ontology
Bibliography

Related articles in PubMed

Variants of Transient Receptor Potential Melastatin Member 4 in Childhood Atrioventricular Block.
Syam N, Chatel S, Ozhathil LC, Sottas V, Rougier JS, Baruteau A, Baron E, Amarouch MY, Daumy X, Probst V, Schott JJ, Abriel H
Journal of the American Heart Association5(5)(2016 May)

Familial Atrial Septal Defect and Sudden Cardiac Death: Identification of a Novel NKX2-5 Mutation and a Review of the Literature.
Elles?e SG, Johansen MM, Bjerre JV, Hjortdal VE, Brunak S, Larsen LA
Congenital heart disease11(3)283-90(2016 May)

Intermolecular Interactions of Cardiac Transcription Factors NKX2.5 and TBX5.
Pradhan L, Gopal S, Li S, Ashur S, Suryanarayanan S, Kasahara H, Nam HJ
Biochemistry55(12)1702-10(2016 Mar)

NKX2-5 mutations in an inbred consanguineous population: genetic and phenotypic diversity.
Abou Hassan OK, Fahed AC, Batrawi M, Arabi M, Refaat MM, DePalma SR, Seidman JG, Seidman CE, Bitar FF, Nemer GM
Scientific reports58848(2015 Mar)

A rat homeobox gene, rNKx-2.5, is a homologue of the tinman gene in Drosophila and is mainly expressed during heart development.
Fu Y, Ruiz-Lozano P, Evans SM
Development genes and evolution207(5)352-358(1997 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data indicated that inhibition of Smad4 diminished both AdBMP2 induced and basal histone acetylation levels in the promoter regions of GATA4 and Nkx2.5.
Title: Smad4 mediated BMP2 signal is essential for the regulation of GATA4 and Nkx2.5 by affecting the histone H3 acetylation in H9c2 cells.

SIRT1 acts as a direct transcriptional target of Nkx2.5 that maintains cardiomyocyte homeostasis and survival.
Title: SIRT1 mediates the protective function of Nkx2.5 during stress in cardiomyocytes.

pacemaking cells originate from Nkx2.5 positive cardiac progenitor cells present in embryonic heart tubes
Title: Endothelin-induced differentiation of Nkx2.5? cardiac progenitor cells into pacemaking cells.

novel function for Nkx2.5 as a repressor of alpha-SMA gene transcription, which may be of homeostatic significance as a means of suppressing myofibroblast differentiation
Title: Nkx2.5/Csx represses myofibroblast differentiation.

GATA-4 and Nkx2. 5 gene expression increased gradually in the course of mesenchymal stem cells differentiation into cardiomyocytes.
Title: [The expression of GATA-4 and Nkx2.5 gene in the transformation of Rattus mesenchymal stem cells into cardiomyocytes].

The following Nkx2-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nkx2-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10203	NM_053651.1 Latest version!	Rattus norvegicus NK2 homeobox 5 (Nkx2-5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nkx2-5 ( NM_053651.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446103.1 Rattus norvegicus NK2 homeobox 5 (Nkx2-5), mRNA.

Price & Availability↑

CloneID	ORa10203
Clone ID Related Accession (Same CDS sequence)	NM_053651.1
Accession Version	NM_053651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-06
Organism	Rattus norvegicus(Norway rat)
Product	homeobox protein Nkx-2.5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF006664.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF006664.1, CK359509.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689596, SAMEA2689600 [ECO:0000348] ##Evidence-Data-END##

ATGTTCCCCA GCCCTGCGCT CACACACACG CCCTTCTCAG TCAAAGACAT CCTGAACCTG 
GAGCAGCAGC AGCGCAGCCT GGCGGCTGGG GACCTGTCTG CGCGCCTCGA GGCCACCCTG 
GCGCCCGCCT CCTGCATGCT GGCCGCCTTC AAGCCGGACG GCTACTCAGG CCCCGAGGCG 
GCAGCGCCCG GCCTGGCAGA GCTGCGCGCG GAGCTGGGCC CCGCGCCTTC GCCCCCCAAG 
TGCTCTCCTG CTTTCCCAAC CGCCCCTACA TTTTATCCGC GAGCCTACGG TGACCCTGAC 
CCCGCCAAGG ACCCTCGGGC GGATAAGAAA GAGCTGTGCG CGCTGCAGAA GGCGGTGGAG 
CTGGACAAAG CCGAGACAGA CGGCGCCGAG AGGCGACGAC CACGGCGGCG ACGGAAGCCA 
CGCGTGCTCT TCTCGCAGGC GCAGGTCTAT GAGCTGGAGC GGCGCTTCAA GCAACAGCGG 
TACCTGTCGC CCGCTGAGCG CGACCAACTG GCCAGCGTGC TGAAGCTCAC GTCCACGCAG 
GTCAAGATCT GGTTCCAGAA CCGCCGCTAC AAGTGTAAGC GACAGCGGCA GGACCAGACT 
CTGGAGCTGC TGGGGCCGCC GCCGCCGCCC GCGCGCAGGA TCGCGGTGCC GGTGTTGGTG 
CGCGACGGGA AGCCCTGCCT GGGGGACTCG GCGGCCTACG CTCCCGCCTA CGGCCTGGGT 
CTCAACGCCT ACGGCTACAA CGCCTACCCC TATCCCGGCT ACGGTGGCGC GGCCTGCAGT 
CCCGCCTACA GCTGCGCCGC GTACCCCGCC GCGCCCCCCG CCGCGCACGC CCCAGCCGCC 
AGCGCCAACA GCAACTTCGT GAACTTTGGC GTCGGGGACT TGAACACGGT GCAGAGTCCC 
GGGATGCCAC AGGGCAATTC GGGCGTCTCC ACGCTGCACG GCATCCGAGC CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446103.1
CDS	94..1050
Translation	MFPSPALTHTPFSVKDILNLEQQQRSLAAGDLSARLEATLAPASCMLAAFKPDGYSGPEAAAPGLAELRAELGPAPSPPKCSPAFPTAPTFYPRAYGDPDPAKDPRADKKELCALQKAVELDKAETDGAERRRPRRRRKPRVLFSQAQVYELERRFKQQRYLSPAERDQLASVLKLTSTQVKIWFQNRRYKCKRQRQDQTLELLGPPPPPARRIAVPVLVRDGKPCLGDSAAYAPAYGLGLNAYGYNAYPYPGYGGAACSPAYSCAAYPAAPPAAHAPAASANSNFVNFGVGDLNTVQSPGMPQGNSGVSTLHGIRAW

Target ORF information:

RefSeq Version	NM_053651.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NK2 homeobox 5 (Nkx2-5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053651.1

ORF Insert Sequence:

ATGTTCCCCA GCCCTGCGCT CACACACACG CCCTTCTCAG TCAAAGACAT CCTGAACCTG 
GAGCAGCAGC AGCGCAGCCT GGCGGCTGGG GACCTGTCTG CGCGCCTCGA GGCCACCCTG 
GCGCCCGCCT CCTGCATGCT GGCCGCCTTC AAGCCGGACG GCTACTCAGG CCCCGAGGCG 
GCAGCGCCCG GCCTGGCAGA GCTGCGCGCG GAGCTGGGCC CCGCGCCTTC GCCCCCCAAG 
TGCTCTCCTG CTTTCCCAAC CGCCCCTACA TTTTATCCGC GAGCCTACGG TGACCCTGAC 
CCCGCCAAGG ACCCTCGGGC GGATAAGAAA GAGCTGTGCG CGCTGCAGAA GGCGGTGGAG 
CTGGACAAAG CCGAGACAGA CGGCGCCGAG AGGCGACGAC CACGGCGGCG ACGGAAGCCA 
CGCGTGCTCT TCTCGCAGGC GCAGGTCTAT GAGCTGGAGC GGCGCTTCAA GCAACAGCGG 
TACCTGTCGC CCGCTGAGCG CGACCAACTG GCCAGCGTGC TGAAGCTCAC GTCCACGCAG 
GTCAAGATCT GGTTCCAGAA CCGCCGCTAC AAGTGTAAGC GACAGCGGCA GGACCAGACT 
CTGGAGCTGC TGGGGCCGCC GCCGCCGCCC GCGCGCAGGA TCGCGGTGCC GGTGTTGGTG 
CGCGACGGGA AGCCCTGCCT GGGGGACTCG GCGGCCTACG CTCCCGCCTA CGGCCTGGGT 
CTCAACGCCT ACGGCTACAA CGCCTACCCC TATCCCGGCT ACGGTGGCGC GGCCTGCAGT 
CCCGCCTACA GCTGCGCCGC GTACCCCGCC GCGCCCCCCG CCGCGCACGC CCCAGCCGCC 
AGCGCCAACA GCAACTTCGT GAACTTTGGC GTCGGGGACT TGAACACGGT GCAGAGTCCC 
GGGATGCCAC AGGGCAATTC GGGCGTCTCC ACGCTGCACG GCATCCGAGC CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Variants of Transient Receptor Potential Melastatin Member 4 in Childhood Atrioventricular Block.
Journal of the American Heart Association5(5)(2016 May)
Syam N,Chatel S,Ozhathil LC,Sottas V,Rougier JS,Baruteau A,Baron E,Amarouch MY,Daumy X,Probst V,Schott JJ,Abriel H

Familial Atrial Septal Defect and Sudden Cardiac Death: Identification of a Novel NKX2-5 Mutation and a Review of the Literature.
Congenital heart disease11(3)283-90(2016 May)
Elles?e SG,Johansen MM,Bjerre JV,Hjortdal VE,Brunak S,Larsen LA

Intermolecular Interactions of Cardiac Transcription Factors NKX2.5 and TBX5.
Biochemistry55(12)1702-10(2016 Mar)
Pradhan L,Gopal S,Li S,Ashur S,Suryanarayanan S,Kasahara H,Nam HJ

NKX2-5 mutations in an inbred consanguineous population: genetic and phenotypic diversity.
Scientific reports58848(2015 Mar)
Abou Hassan OK,Fahed AC,Batrawi M,Arabi M,Refaat MM,DePalma SR,Seidman JG,Seidman CE,Bitar FF,Nemer GM

A rat homeobox gene, rNKx-2.5, is a homologue of the tinman gene in Drosophila and is mainly expressed during heart development.
Development genes and evolution207(5)352-358(1997 Nov)
Fu Y,Ruiz-Lozano P,Evans SM

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Nkx2-5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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