Pitx1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pitx1
Entrez Gene ID	113983
Full Name	paired-like homeodomain 1
Synonyms	Bft
General protein information	Preferred Names paired-like homeodomain 1 Names pituitary homeobox 1 homeobox protein PITX1 paired-like homeodomain transcription factor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p14
Summary	paired-like homeobox transcription factor; transcription regulator of pituitary genes; genetic marker for nascent Rathke's pouch, precursor of anterior and intermediate lobes of pituitary gland [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053624.2	NP_446076.1	pituitary homeobox 1
XM_006253569.3	XP_006253631.1	pituitary homeobox 1 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pitx1	NP_446076.1
Xenopus tropicalis (tropical clawed frog)	pitx1	NP_001007500.1
Mus musculus (house mouse)	Pitx1	NP_035227.1
Homo sapiens (human)	PITX1	NP_002644.4
Canis lupus familiaris (dog)	PITX1	XP_005626613.1
Pan troglodytes (chimpanzee)	PITX1	XP_001168135.1
	PITX1	XP_002804564.1
Bos taurus (cattle)	PITX1	NP_001192229.1
Gallus gallus (chicken)	PITX1	NP_001161158.1
Danio rerio (zebrafish)	pitx1	NP_001035436.3

Gene Ontology
Bibliography

Related articles in PubMed

Functional characterization of a human POU1F1 mutation associated with isolated growth hormone deficiency: a novel etiology for IGHD.
Sobrier ML, Tsai YC, P?rez C, Leheup B, Bouceba T, Duquesnoy P, Copin B, Sizova D, Penzo A, Stanger BZ, Cooke NE, Liebhaber SA, Amselem S
Human molecular genetics25(3)472-83(2016 Feb)

The tumor suppressor HHEX inhibits axon growth when prematurely expressed in developing central nervous system neurons.
Simpson MT, Venkatesh I, Callif BL, Thiel LK, Coley DM, Winsor KN, Wang Z, Kramer AA, Lerch JK, Blackmore MG
Molecular and cellular neurosciences68272-83(2015 Sep)

In vivo oestrogenic modulation of Egr1 and Pitx1 gene expression in female rat pituitary gland.
Gajewska A, Herman AP, Woli?ska-Witort E, Kochman K, Zwierzchowski L
Journal of molecular endocrinology53(3)355-66(2014 Dec)

Pitx1 is necessary for normal initiation of hindlimb outgrowth through regulation of Tbx4 expression and shapes hindlimb morphologies via targeted growth control.
Duboc V, Logan MP
Development (Cambridge, England)138(24)5301-9(2011 Dec)

Identification of PITX1 as a TERT suppressor gene located on human chromosome 5.
Qi DL, Ohhira T, Fujisaki C, Inoue T, Ohta T, Osaki M, Ohshiro E, Seko T, Aoki S, Oshimura M, Kugoh H
Molecular and cellular biology31(8)1624-36(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study idnetifed that the PITX1 as inhibitors of neurite outgrowth.
Title: The tumor suppressor HHEX inhibits axon growth when prematurely expressed in developing central nervous system neurons.

Orchestrated Egr1 and Pitx1 activities depending on specific central and peripheral regulatory inputs could be responsible for physiologically variable Lhb gene promoter activation in vivo.
Title: In vivo oestrogenic modulation of Egr1 and Pitx1 gene expression in female rat pituitary gland.

identify a second functional Pitx1 region spanning positions -73 to -52 in the LHbeta gene promoter
Title: Pituitary homeobox 1 (Pitx1) stimulates rat LHbeta gene expression via two functional DNA-regulatory regions.

the paired-like homeobox transcription factors Pitx1 and Pitx2 are factors functionally activating the proximal human prolactin promoter (hPRL-164luc)
Title: Pitx factors are involved in basal and hormone-regulated activity of the human prolactin promoter.

Pituitary homeobox 1 activates the rat FSHbeta (rFSHbeta) gene through both direct and indirect interactions with the rFSHbeta gene promoter.
Title: Pituitary homeobox 1 activates the rat FSHbeta (rFSHbeta) gene through both direct and indirect interactions with the rFSHbeta gene promoter.

The following Pitx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pitx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10118	NM_053624.2 Latest version!	Rattus norvegicus paired-like homeodomain 1 (Pitx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa10118	XM_006253569.3 Latest version!	Rattus norvegicus paired-like homeodomain 1 (Pitx1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pitx1 ( NM_053624.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446076.1 Rattus norvegicus paired-like homeodomain 1 (Pitx1), mRNA.

Price & Availability↑

CloneID	ORa10118
Clone ID Related Accession (Same CDS sequence)	NM_053624.2 , XM_006253569.3
Accession Version	NM_053624.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	pituitary homeobox 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061966.1. On Dec 20, 2003 this sequence version replaced NM_053624.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061966.1, AB047298.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348, SAMN01906349 [ECO:0000348] ##Evidence-Data-END##

ATGGACGCCT TCAAGGGAGG CATGAGCCTG GAGAGGCTAC CGGAGGGGCT GCGGCCACCG 
CCACCGCCGC CACACGACAT GGGGCCCAGC TTCCACCTGG CCCGCACCGC CGACCCCCGG 
GAGCCGCTGG AGAACTCAGC CAGCGAGTCA TCCGACGCCG ATCTGCCAGA CAAGGAGCGC 
GGTGGAGAGG CCAAGGTGCC TGAGGATGGC GGCGCGGGCA GCGCTGGCTG CGGCAGCGCG 
GAAGACCCAG CCAAGAAGAA GAAACAGCGG CGTCAACGCA CTCACTTCAC AAGCCAGCAG 
TTGCAAGAGC TGGAGGCCAC GTTCCAAAGG AACCGCTACC CGGACATGAG CATGAGAGAG 
GAGATCGCGG TGTGGACCAA CCTCACTGAG CCGCGAGTGC GGGTCTGGTT CAAGAACCGG 
CGAGCCAAGT GGCGCAAGAG GGAGCGGAAC CAGCAGTTGG ACCTGTGCAA GGGCGGCTAC 
GTGCCGCAGT TCAGCGGCTT GGTGCAGCCC TACGAGGACG TGTACGCCGC CGCCGGCTAC 
TCCTACAACA ACTGGGCCGC CAAGAGCTTG GCACCGGCGC CGCTGTCCAC CAAGAGCTTT 
ACCTTCTTCA ACTCCATGAG TCCGCTATCC TCTCAGTCCA TGTTCTCGGC CCCCAGCTCC 
ATCTCTTCCA TGACCATGCC ATCCAGCATG GGCCCGGGCT CCGTGCCCGG CATGCCCAAC 
TCGGGCCTCA ACAACATCAA CAACCTCACG GGCTCCTCGC TCAACTCGGC CATGTCGCCG 
GGCGCCTGCC CCTACGGCAC CCCCGCCTCG CCCTACAGCG TCTACCGGGA CACGTGCAAC 
TCGAGCCTCG CCAGCCTGCG GCTCAAGTCC AAGCAGCACT CCTCGTTTGG CTACGGCGGT 
CTGCAGGGTC CAGCGTCGGG CCTCAACGCC TGCCAGTATA ACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446076.1
CDS	292..1239
Translation	MDAFKGGMSLERLPEGLRPPPPPPHDMGPSFHLARTADPREPLENSASESSDADLPDKERGGEAKVPEDGGAGSAGCGSAEDPAKKKKQRRQRTHFTSQQLQELEATFQRNRYPDMSMREEIAVWTNLTEPRVRVWFKNRRAKWRKRERNQQLDLCKGGYVPQFSGLVQPYEDVYAAAGYSYNNWAAKSLAPAPLSTKSFTFFNSMSPLSSQSMFSAPSSISSMTMPSSMGPGSVPGMPNSGLNNINNLTGSSLNSAMSPGACPYGTPASPYSVYRDTCNSSLASLRLKSKQHSSFGYGGLQGPASGLNACQYNS

Target ORF information:

RefSeq Version	NM_053624.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus paired-like homeodomain 1 (Pitx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053624.2

ORF Insert Sequence:

ATGGACGCCT TCAAGGGAGG CATGAGCCTG GAGAGGCTAC CGGAGGGGCT GCGGCCACCG 
CCACCGCCGC CACACGACAT GGGGCCCAGC TTCCACCTGG CCCGCACCGC CGACCCCCGG 
GAGCCGCTGG AGAACTCAGC CAGCGAGTCA TCCGACGCCG ATCTGCCAGA CAAGGAGCGC 
GGTGGAGAGG CCAAGGTGCC TGAGGATGGC GGCGCGGGCA GCGCTGGCTG CGGCAGCGCG 
GAAGACCCAG CCAAGAAGAA GAAACAGCGG CGTCAACGCA CTCACTTCAC AAGCCAGCAG 
TTGCAAGAGC TGGAGGCCAC GTTCCAAAGG AACCGCTACC CGGACATGAG CATGAGAGAG 
GAGATCGCGG TGTGGACCAA CCTCACTGAG CCGCGAGTGC GGGTCTGGTT CAAGAACCGG 
CGAGCCAAGT GGCGCAAGAG GGAGCGGAAC CAGCAGTTGG ACCTGTGCAA GGGCGGCTAC 
GTGCCGCAGT TCAGCGGCTT GGTGCAGCCC TACGAGGACG TGTACGCCGC CGCCGGCTAC 
TCCTACAACA ACTGGGCCGC CAAGAGCTTG GCACCGGCGC CGCTGTCCAC CAAGAGCTTT 
ACCTTCTTCA ACTCCATGAG TCCGCTATCC TCTCAGTCCA TGTTCTCGGC CCCCAGCTCC 
ATCTCTTCCA TGACCATGCC ATCCAGCATG GGCCCGGGCT CCGTGCCCGG CATGCCCAAC 
TCGGGCCTCA ACAACATCAA CAACCTCACG GGCTCCTCGC TCAACTCGGC CATGTCGCCG 
GGCGCCTGCC CCTACGGCAC CCCCGCCTCG CCCTACAGCG TCTACCGGGA CACGTGCAAC 
TCGAGCCTCG CCAGCCTGCG GCTCAAGTCC AAGCAGCACT CCTCGTTTGG CTACGGCGGT 
CTGCAGGGTC CAGCGTCGGG CCTCAACGCC TGCCAGTATA ACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pitx1 ( XM_006253569.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253631.1 Rattus norvegicus paired-like homeodomain 1 (Pitx1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa10118
Clone ID Related Accession (Same CDS sequence)	NM_053624.2 , XM_006253569.3
Accession Version	XM_006253569.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pituitary homeobox 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253569.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCCT TCAAGGGAGG CATGAGCCTG GAGAGGCTAC CGGAGGGGCT GCGGCCACCG 
CCACCGCCGC CACACGACAT GGGGCCCAGC TTCCACCTGG CCCGCACCGC CGACCCCCGG 
GAGCCGCTGG AGAACTCAGC CAGCGAGTCA TCCGACGCCG ATCTGCCAGA CAAGGAGCGC 
GGTGGAGAGG CCAAGGTGCC TGAGGATGGC GGCGCGGGCA GCGCTGGCTG CGGCAGCGCG 
GAAGACCCAG CCAAGAAGAA GAAACAGCGG CGTCAACGCA CTCACTTCAC AAGCCAGCAG 
TTGCAAGAGC TGGAGGCCAC GTTCCAAAGG AACCGCTACC CGGACATGAG CATGAGAGAG 
GAGATCGCGG TGTGGACCAA CCTCACTGAG CCGCGAGTGC GGGTCTGGTT CAAGAACCGG 
CGAGCCAAGT GGCGCAAGAG GGAGCGGAAC CAGCAGTTGG ACCTGTGCAA GGGCGGCTAC 
GTGCCGCAGT TCAGCGGCTT GGTGCAGCCC TACGAGGACG TGTACGCCGC CGCCGGCTAC 
TCCTACAACA ACTGGGCCGC CAAGAGCTTG GCACCGGCGC CGCTGTCCAC CAAGAGCTTT 
ACCTTCTTCA ACTCCATGAG TCCGCTATCC TCTCAGTCCA TGTTCTCGGC CCCCAGCTCC 
ATCTCTTCCA TGACCATGCC ATCCAGCATG GGCCCGGGCT CCGTGCCCGG CATGCCCAAC 
TCGGGCCTCA ACAACATCAA CAACCTCACG GGCTCCTCGC TCAACTCGGC CATGTCGCCG 
GGCGCCTGCC CCTACGGCAC CCCCGCCTCG CCCTACAGCG TCTACCGGGA CACGTGCAAC 
TCGAGCCTCG CCAGCCTGCG GCTCAAGTCC AAGCAGCACT CCTCGTTTGG CTACGGCGGT 
CTGCAGGGTC CAGCGTCGGG CCTCAACGCC TGCCAGTATA ACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253631.1
CDS	110..1057
Translation	MDAFKGGMSLERLPEGLRPPPPPPHDMGPSFHLARTADPREPLENSASESSDADLPDKERGGEAKVPEDGGAGSAGCGSAEDPAKKKKQRRQRTHFTSQQLQELEATFQRNRYPDMSMREEIAVWTNLTEPRVRVWFKNRRAKWRKRERNQQLDLCKGGYVPQFSGLVQPYEDVYAAAGYSYNNWAAKSLAPAPLSTKSFTFFNSMSPLSSQSMFSAPSSISSMTMPSSMGPGSVPGMPNSGLNNINNLTGSSLNSAMSPGACPYGTPASPYSVYRDTCNSSLASLRLKSKQHSSFGYGGLQGPASGLNACQYNS

Target ORF information:

RefSeq Version	XM_006253569.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus paired-like homeodomain 1 (Pitx1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253569.3

ORF Insert Sequence:

ATGGACGCCT TCAAGGGAGG CATGAGCCTG GAGAGGCTAC CGGAGGGGCT GCGGCCACCG 
CCACCGCCGC CACACGACAT GGGGCCCAGC TTCCACCTGG CCCGCACCGC CGACCCCCGG 
GAGCCGCTGG AGAACTCAGC CAGCGAGTCA TCCGACGCCG ATCTGCCAGA CAAGGAGCGC 
GGTGGAGAGG CCAAGGTGCC TGAGGATGGC GGCGCGGGCA GCGCTGGCTG CGGCAGCGCG 
GAAGACCCAG CCAAGAAGAA GAAACAGCGG CGTCAACGCA CTCACTTCAC AAGCCAGCAG 
TTGCAAGAGC TGGAGGCCAC GTTCCAAAGG AACCGCTACC CGGACATGAG CATGAGAGAG 
GAGATCGCGG TGTGGACCAA CCTCACTGAG CCGCGAGTGC GGGTCTGGTT CAAGAACCGG 
CGAGCCAAGT GGCGCAAGAG GGAGCGGAAC CAGCAGTTGG ACCTGTGCAA GGGCGGCTAC 
GTGCCGCAGT TCAGCGGCTT GGTGCAGCCC TACGAGGACG TGTACGCCGC CGCCGGCTAC 
TCCTACAACA ACTGGGCCGC CAAGAGCTTG GCACCGGCGC CGCTGTCCAC CAAGAGCTTT 
ACCTTCTTCA ACTCCATGAG TCCGCTATCC TCTCAGTCCA TGTTCTCGGC CCCCAGCTCC 
ATCTCTTCCA TGACCATGCC ATCCAGCATG GGCCCGGGCT CCGTGCCCGG CATGCCCAAC 
TCGGGCCTCA ACAACATCAA CAACCTCACG GGCTCCTCGC TCAACTCGGC CATGTCGCCG 
GGCGCCTGCC CCTACGGCAC CCCCGCCTCG CCCTACAGCG TCTACCGGGA CACGTGCAAC 
TCGAGCCTCG CCAGCCTGCG GCTCAAGTCC AAGCAGCACT CCTCGTTTGG CTACGGCGGT 
CTGCAGGGTC CAGCGTCGGG CCTCAACGCC TGCCAGTATA ACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional characterization of a human POU1F1 mutation associated with isolated growth hormone deficiency: a novel etiology for IGHD.
Human molecular genetics25(3)472-83(2016 Feb)
Sobrier ML,Tsai YC,P?rez C,Leheup B,Bouceba T,Duquesnoy P,Copin B,Sizova D,Penzo A,Stanger BZ,Cooke NE,Liebhaber SA,Amselem S

The tumor suppressor HHEX inhibits axon growth when prematurely expressed in developing central nervous system neurons.
Molecular and cellular neurosciences68272-83(2015 Sep)
Simpson MT,Venkatesh I,Callif BL,Thiel LK,Coley DM,Winsor KN,Wang Z,Kramer AA,Lerch JK,Blackmore MG

In vivo oestrogenic modulation of Egr1 and Pitx1 gene expression in female rat pituitary gland.
Journal of molecular endocrinology53(3)355-66(2014 Dec)
Gajewska A,Herman AP,Woli?ska-Witort E,Kochman K,Zwierzchowski L

Pitx1 is necessary for normal initiation of hindlimb outgrowth through regulation of Tbx4 expression and shapes hindlimb morphologies via targeted growth control.
Development (Cambridge, England)138(24)5301-9(2011 Dec)
Duboc V,Logan MP

Identification of PITX1 as a TERT suppressor gene located on human chromosome 5.
Molecular and cellular biology31(8)1624-36(2011 Apr)
Qi DL,Ohhira T,Fujisaki C,Inoue T,Ohta T,Osaki M,Ohshiro E,Seko T,Aoki S,Oshimura M,Kugoh H

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Pitx1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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